AlfalfaGEDB | MS.gene038469

M. sativa cultivar XinJiangDaYe / MS.gene038469

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038469.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038469.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038469.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene038469	MS.gene66225	PPI
MS.gene035719	MS.gene038469	PPI
MS.gene038469	MS.gene035720	PPI
MS.gene038469	MS.gene70955	PPI
MS.gene038469	MS.gene61017	PPI
MS.gene58854	MS.gene038469	PPI
MS.gene038469	MS.gene09871	PPI
MS.gene038469	MS.gene012390	PPI
MS.gene038469	MS.gene08121	PPI
MS.gene30920	MS.gene038469	PPI
MS.gene038469	MS.gene064557	PPI
MS.gene038469	MS.gene049705	PPI
MS.gene061872	MS.gene038469	PPI
MS.gene038469	MS.gene04434	PPI
MS.gene038469	MS.gene070907	PPI
MS.gene038469	MS.gene04392	PPI
MS.gene88680	MS.gene038469	PPI
MS.gene038469	MS.gene010219	PPI
MS.gene070241	MS.gene038469	PPI
MS.gene056215	MS.gene038469	PPI
MS.gene038469	MS.gene040383	PPI
MS.gene038469	MS.gene68545	PPI
MS.gene038469	MS.gene070242	PPI
MS.gene038469	MS.gene016624	PPI
MS.gene061876	MS.gene038469	PPI
MS.gene012391	MS.gene038469	PPI
MS.gene038469	MS.gene048746	PPI
MS.gene060463	MS.gene038469	PPI
MS.gene038469	MS.gene043967	PPI
MS.gene038469	MS.gene08053	PPI
MS.gene038469	MS.gene035897	PPI
MS.gene038469	MS.gene05608	PPI
MS.gene038469	MS.gene84536	PPI
MS.gene038469	MS.gene035896	PPI
MS.gene038469	MS.gene82546	PPI
MS.gene88681	MS.gene038469	PPI
MS.gene070244	MS.gene038469	PPI
MS.gene038469	MS.gene60093	PPI
MS.gene038469	MS.gene08122	PPI
MS.gene038469	MS.gene09873	PPI
MS.gene038469	MS.gene022932	PPI
MS.gene009027	MS.gene038469	PPI
MS.gene31196	MS.gene038469	PPI
MS.gene038469	MS.gene010220	PPI
MS.gene038469	MS.gene04972	PPI
MS.gene038469	MS.gene004121	PPI
MS.gene060465	MS.gene038469	PPI
MS.gene038469	MS.gene62520	PPI
MS.gene038469	MS.gene018578	PPI
MS.gene038469	MS.gene035721	PPI
MS.gene038469	MS.gene04064	PPI
MS.gene028629	MS.gene038469	PPI
MS.gene038469	MS.gene018580	PPI
MS.gene038469	MS.gene004124	PPI
MS.gene038469	MS.gene072493	PPI
MS.gene71215	MS.gene038469	PPI
MS.gene038469	MS.gene04052	PPI
MS.gene061870	MS.gene038469	PPI
MS.gene038469	MS.gene061866	PPI
MS.gene038469	MS.gene64650	PPI
MS.gene038469	MS.gene79195	PPI
MS.gene038469	MS.gene048705	PPI
MS.gene035718	MS.gene038469	PPI
MS.gene038469	MS.gene061874	PPI
MS.gene038469	MS.gene04576	PPI
MS.gene038469	MS.gene061869	PPI
MS.gene071136	MS.gene038469	PPI
MS.gene038469	MS.gene64920	PPI
MS.gene78578	MS.gene038469	PPI
MS.gene038469	MS.gene04452	PPI
MS.gene038469	MS.gene061867	PPI
MS.gene038469	MS.gene008276	PPI
MS.gene04433	MS.gene038469	PPI
MS.gene038469	MS.gene05619	PPI
MS.gene038469	MS.gene73136	PPI
MS.gene038469	MS.gene049821	PPI
MS.gene035787	MS.gene038469	PPI
MS.gene04067	MS.gene038469	PPI
MS.gene038469	MS.gene04068	PPI
MS.gene038469	MS.gene022946	PPI
MS.gene038469	MS.gene035719	PPI
MS.gene038469	MS.gene05623	PPI
MS.gene038469	MS.gene89488	PPI
MS.gene89487	MS.gene038469	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038469.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene038469.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene038469.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene038469.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene038469.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene038469.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene038469.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene038469.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene038469.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene038469.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene038469.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene038469.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene038469.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene038469.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene038469.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene038469.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038469.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene038469.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene038469.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene038469.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene038469.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene038469.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene038469.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene038469.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene038469.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene038469.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene038469.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 25 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCCTGCGATTCGTCGTT+TGG	0.336158	8.4:-36209267	MS.gene038469:CDS
GGGAGAACTCTTTACGGTTT+CGG	0.376970	8.4:-36209077	MS.gene038469:CDS
TCGCAGGCTTTGTTATTCCT+TGG	0.407291	8.4:+36209279	None:intergenic
GGAAAAGGAGGAAAAGGTTT+AGG	0.411601	8.4:-36209347	MS.gene038469:CDS
AAAATGTCAGGTCGCGGAAA+AGG	0.432832	8.4:-36209362	MS.gene038469:CDS
AGACAGGGGAGAACTCTTTA+CGG	0.442868	8.4:-36209083	MS.gene038469:CDS
TGTTTATGCTTTGAAGAGAC+AGG	0.461410	8.4:-36209099	MS.gene038469:CDS
GGAGGAAAAGGTTTAGGAAA+GGG	0.485908	8.4:-36209341	MS.gene038469:CDS
CGTCGTTTGGCTCGAAGAGG+AGG	0.516708	8.4:-36209254	MS.gene038469:CDS
TTACTTACACTGAGCATGCT+AGG	0.518049	8.4:-36209148	MS.gene038469:CDS
AGGAGGAAAAGGTTTAGGAA+AGG	0.527814	8.4:-36209342	MS.gene038469:CDS
GGTCGCGGAAAAGGAGGAAA+AGG	0.538751	8.4:-36209353	MS.gene038469:CDS
CGAGCCAAACGACGAATCGC+AGG	0.562444	8.4:+36209263	None:intergenic
AGGAGCAAAGAGGCACAGAA+AGG	0.564264	8.4:-36209318	MS.gene038469:CDS
GGAAAAGGTTTAGGAAAGGG+AGG	0.575812	8.4:-36209338	MS.gene038469:CDS
ATGTCAGGTCGCGGAAAAGG+AGG	0.578066	8.4:-36209359	MS.gene038469:CDS
GTTTATGCTTTGAAGAGACA+GGG	0.590468	8.4:-36209098	MS.gene038469:CDS
GGAGGTGTGAAGAGAATCAG+TGG	0.594114	8.4:-36209236	MS.gene038469:CDS
AGAACTCTTTACGGTTTCGG+TGG	0.599890	8.4:-36209074	MS.gene038469:CDS
ATTCGTCGTTTGGCTCGAAG+AGG	0.614299	8.4:-36209257	MS.gene038469:CDS
GTTCTTCGTGACAACATCCA+AGG	0.625870	8.4:-36209296	MS.gene038469:CDS
TTTATGCTTTGAAGAGACAG+GGG	0.664122	8.4:-36209097	MS.gene038469:CDS
TAGGAAAGGGAGGAGCAAAG+AGG	0.677223	8.4:-36209328	MS.gene038469:CDS
AGCATAAACAACATCCATAG+CGG	0.701201	8.4:+36209112	None:intergenic
CTTATCTATGAAGAAACACG+CGG	0.709082	8.4:-36209212	MS.gene038469:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGCATAAACAACATCCATAG+CGG	+	chr8.4:36209320-36209339	None:intergenic	35.0%
	CTTATCTATGAAGAAACACG+CGG	-	chr8.4:36209217-36209236	MS.gene038469:CDS	35.0%
!!	GTTTATGCTTTGAAGAGACA+GGG	-	chr8.4:36209331-36209350	MS.gene038469:CDS	35.0%
!!	TGTTTATGCTTTGAAGAGAC+AGG	-	chr8.4:36209330-36209349	MS.gene038469:CDS	35.0%
!!	TTTATGCTTTGAAGAGACAG+GGG	-	chr8.4:36209332-36209351	MS.gene038469:CDS	35.0%
	AGGAGGAAAAGGTTTAGGAA+AGG	-	chr8.4:36209087-36209106	MS.gene038469:CDS	40.0%
	GAGAAAAACTGTTACCGCTA+TGG	-	chr8.4:36209303-36209322	MS.gene038469:CDS	40.0%
	GGAAAAGGAGGAAAAGGTTT+AGG	-	chr8.4:36209082-36209101	MS.gene038469:CDS	40.0%
	GGAGGAAAAGGTTTAGGAAA+GGG	-	chr8.4:36209088-36209107	MS.gene038469:CDS	40.0%
	TTACTTACACTGAGCATGCT+AGG	-	chr8.4:36209281-36209300	MS.gene038469:CDS	40.0%
	AGAACTCTTTACGGTTTCGG+TGG	-	chr8.4:36209355-36209374	MS.gene038469:CDS	45.0%
	AGACAGGGGAGAACTCTTTA+CGG	-	chr8.4:36209346-36209365	MS.gene038469:CDS	45.0%
	GGAAAAGGTTTAGGAAAGGG+AGG	-	chr8.4:36209091-36209110	MS.gene038469:CDS	45.0%
	GGGAGAACTCTTTACGGTTT+CGG	-	chr8.4:36209352-36209371	MS.gene038469:CDS	45.0%
	GTTCTTCGTGACAACATCCA+AGG	-	chr8.4:36209133-36209152	MS.gene038469:CDS	45.0%
!!	TCGCAGGCTTTGTTATTCCT+TGG	+	chr8.4:36209153-36209172	None:intergenic	45.0%
	AAAGCCTGCGATTCGTCGTT+TGG	-	chr8.4:36209162-36209181	MS.gene038469:CDS	50.0%
	TAGGAAAGGGAGGAGCAAAG+AGG	-	chr8.4:36209101-36209120	MS.gene038469:CDS	50.0%
!	AGGAGCAAAGAGGCACAGAA+AGG	-	chr8.4:36209111-36209130	MS.gene038469:CDS	50.0%
!	ATTCGTCGTTTGGCTCGAAG+AGG	-	chr8.4:36209172-36209191	MS.gene038469:CDS	50.0%
!	GGAGGTGTGAAGAGAATCAG+TGG	-	chr8.4:36209193-36209212	MS.gene038469:CDS	50.0%
	ATGTCAGGTCGCGGAAAAGG+AGG	-	chr8.4:36209070-36209089	MS.gene038469:CDS	55.0%
	GGTCGCGGAAAAGGAGGAAA+AGG	-	chr8.4:36209076-36209095	MS.gene038469:CDS	55.0%
	CGAGCCAAACGACGAATCGC+AGG	+	chr8.4:36209169-36209188	None:intergenic	60.0%
!	CGTCGTTTGGCTCGAAGAGG+AGG	-	chr8.4:36209175-36209194	MS.gene038469:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	36209070	36209381	36209070	ID=MS.gene038469
chr8.4	mRNA	36209070	36209381	36209070	ID=MS.gene038469.t1;Parent=MS.gene038469
chr8.4	exon	36209070	36209381	36209070	ID=MS.gene038469.t1.exon1;Parent=MS.gene038469.t1
chr8.4	CDS	36209070	36209381	36209070	ID=cds.MS.gene038469.t1;Parent=MS.gene038469.t1

Gene Sequence	>MS.gene038469 ATGTCAGGTCGCGGAAAAGGAGGAAAAGGTTTAGGAAAGGGAGGAGCAAAGAGGCACAGAAAGGTTCTTCGTGACAACATCCAAGGAATAACAAAGCCTGCGATTCGTCGTTTGGCTCGAAGAGGAGGTGTGAAGAGAATCAGTGGTCTTATCTATGAAGAAACACGCGGTGTTCTTAAGATCTTTCTTGAGAACGTTATTCGTGATGCTGTTACTTACACTGAGCATGCTAGGAGAAAAACTGTTACCGCTATGGATGTTGTTTATGCTTTGAAGAGACAGGGGAGAACTCTTTACGGTTTCGGTGGTTGA
Protein sequence	>MS.gene038469.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG