AlfalfaGEDB | MS.gene30920

M. sativa cultivar XinJiangDaYe / MS.gene30920

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30920.t1	PPD70927.1	95.5	112	4	1	1	111	1	112	2.40E-49	205.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30920.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	200.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30920.t1	A0A287GP14	93.0	114	8	0	1	114	20	133	4.9e-49	203.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene30920	0.832249	1.05E-55	-1.69E-46
MS.gene049159	MS.gene30920	0.822483	2.31E-53	-1.69E-46
MS.gene049254	MS.gene30920	0.840934	6.45E-58	-1.69E-46
MS.gene049343	MS.gene30920	0.816739	4.73E-52	-1.69E-46
MS.gene049404	MS.gene30920	0.813113	3.02E-51	-1.69E-46
MS.gene049501	MS.gene30920	0.826565	2.53E-54	-1.69E-46
MS.gene049821	MS.gene30920	0.864881	8.83E-65	-1.69E-46
MS.gene049825	MS.gene30920	0.81528	1.00E-51	-1.69E-46
MS.gene049850	MS.gene30920	0.819137	1.36E-52	-1.69E-46
MS.gene049873	MS.gene30920	0.839714	1.34E-57	-1.69E-46
MS.gene050156	MS.gene30920	0.811659	6.27E-51	-1.69E-46
MS.gene050201	MS.gene30920	0.815906	7.27E-52	-1.69E-46
MS.gene050303	MS.gene30920	0.812606	3.89E-51	-1.69E-46
MS.gene050355	MS.gene30920	0.806475	8.08E-50	-1.69E-46
MS.gene05045	MS.gene30920	0.817215	3.70E-52	-1.69E-46
MS.gene050719	MS.gene30920	0.81556	8.68E-52	-1.69E-46
MS.gene050721	MS.gene30920	0.8146	1.42E-51	-1.69E-46
MS.gene050724	MS.gene30920	0.802166	6.38E-49	-1.69E-46
MS.gene050750	MS.gene30920	0.800396	1.47E-48	-1.69E-46
MS.gene050807	MS.gene30920	0.812934	3.30E-51	-1.69E-46
MS.gene050815	MS.gene30920	0.809635	1.72E-50	-1.69E-46
MS.gene050844	MS.gene30920	0.801769	7.70E-49	-1.69E-46
MS.gene050901	MS.gene30920	0.848389	6.32E-60	-1.69E-46
MS.gene050953	MS.gene30920	0.801369	9.30E-49	-1.69E-46
MS.gene051247	MS.gene30920	0.861565	9.38E-64	-1.69E-46
MS.gene051328	MS.gene30920	0.832868	7.40E-56	-1.69E-46
MS.gene051594	MS.gene30920	0.810414	1.17E-50	-1.69E-46
MS.gene051700	MS.gene30920	0.82924	5.75E-55	-1.69E-46
MS.gene051719	MS.gene30920	0.85287	3.47E-61	-1.69E-46
MS.gene051720	MS.gene30920	0.840892	6.61E-58	-1.69E-46
MS.gene051744	MS.gene30920	0.847586	1.05E-59	-1.69E-46
MS.gene051957	MS.gene30920	0.827489	1.52E-54	-1.69E-46
MS.gene051975	MS.gene30920	0.81009	1.37E-50	-1.69E-46
MS.gene052012	MS.gene30920	0.846162	2.58E-59	-1.69E-46
MS.gene052133	MS.gene30920	0.803162	3.98E-49	-1.69E-46
MS.gene052516	MS.gene30920	0.819574	1.08E-52	-1.69E-46
MS.gene052519	MS.gene30920	0.864868	8.92E-65	-1.69E-46
MS.gene052582	MS.gene30920	0.817493	3.21E-52	-1.69E-46
MS.gene052584	MS.gene30920	0.837331	5.54E-57	-1.69E-46
MS.gene052773	MS.gene30920	0.843601	1.27E-58	-1.69E-46
MS.gene052863	MS.gene30920	0.816899	4.36E-52	-1.69E-46
MS.gene052867	MS.gene30920	0.817219	3.69E-52	-1.69E-46
MS.gene052896	MS.gene30920	0.803911	2.78E-49	-1.69E-46
MS.gene052947	MS.gene30920	0.80841	3.14E-50	-1.69E-46
MS.gene053006	MS.gene30920	0.807947	3.94E-50	-1.69E-46
MS.gene053008	MS.gene30920	0.857987	1.12E-62	-1.69E-46
MS.gene053054	MS.gene30920	-0.828696	7.79E-55	-1.69E-46
MS.gene053145	MS.gene30920	0.80223	6.19E-49	-1.69E-46
MS.gene053226	MS.gene30920	0.869702	2.54E-66	-1.69E-46
MS.gene053599	MS.gene30920	0.801082	1.07E-48	-1.69E-46
MS.gene05364	MS.gene30920	0.819461	1.15E-52	-1.69E-46
MS.gene053883	MS.gene30920	0.815747	7.89E-52	-1.69E-46
MS.gene05414	MS.gene30920	0.805297	1.43E-49	-1.69E-46
MS.gene05415	MS.gene30920	0.830093	3.57E-55	-1.69E-46
MS.gene054236	MS.gene30920	0.817976	2.49E-52	-1.69E-46
MS.gene05423	MS.gene30920	0.809769	1.61E-50	-1.69E-46
MS.gene05458	MS.gene30920	0.803889	2.81E-49	-1.69E-46
MS.gene054651	MS.gene30920	0.84141	4.83E-58	-1.69E-46
MS.gene054657	MS.gene30920	0.818863	1.57E-52	-1.69E-46
MS.gene054775	MS.gene30920	0.843266	1.56E-58	-1.69E-46
MS.gene05517	MS.gene30920	0.809251	2.07E-50	-1.69E-46
MS.gene055466	MS.gene30920	0.832884	7.33E-56	-1.69E-46
MS.gene055468	MS.gene30920	0.821322	4.29E-53	-1.69E-46
MS.gene055512	MS.gene30920	0.80705	6.11E-50	-1.69E-46
MS.gene055513	MS.gene30920	0.815587	8.57E-52	-1.69E-46
MS.gene055586	MS.gene30920	0.808016	3.81E-50	-1.69E-46
MS.gene055589	MS.gene30920	0.830065	3.62E-55	-1.69E-46
MS.gene055623	MS.gene30920	0.822411	2.40E-53	-1.69E-46
MS.gene055694	MS.gene30920	0.801727	7.86E-49	-1.69E-46
MS.gene055778	MS.gene30920	0.830665	2.58E-55	-1.69E-46
MS.gene055808	MS.gene30920	0.838989	2.07E-57	-1.69E-46
MS.gene055818	MS.gene30920	0.870622	1.27E-66	-1.69E-46
MS.gene055841	MS.gene30920	0.824733	6.88E-54	-1.69E-46
MS.gene055926	MS.gene30920	0.801186	1.01E-48	-1.69E-46
MS.gene05604	MS.gene30920	0.828357	9.40E-55	-1.69E-46
MS.gene056091	MS.gene30920	0.801177	1.02E-48	-1.69E-46
MS.gene056162	MS.gene30920	0.80255	5.32E-49	-1.69E-46
MS.gene056174	MS.gene30920	0.800094	1.69E-48	-1.69E-46
MS.gene056215	MS.gene30920	0.871585	6.11E-67	-1.69E-46
MS.gene056308	MS.gene30920	0.819345	1.22E-52	-1.69E-46
MS.gene056366	MS.gene30920	0.863913	1.77E-64	-1.69E-46
MS.gene056498	MS.gene30920	0.814197	1.74E-51	-1.69E-46
MS.gene056595	MS.gene30920	0.836056	1.17E-56	-1.69E-46
MS.gene056631	MS.gene30920	0.800668	1.29E-48	-1.69E-46
MS.gene056776	MS.gene30920	0.831726	1.42E-55	-1.69E-46
MS.gene05690	MS.gene30920	0.812441	4.23E-51	-1.69E-46
MS.gene056986	MS.gene30920	0.815483	9.03E-52	-1.69E-46
MS.gene057026	MS.gene30920	0.824799	6.63E-54	-1.69E-46
MS.gene057034	MS.gene30920	0.813323	2.71E-51	-1.69E-46
MS.gene057060	MS.gene30920	0.84102	6.12E-58	-1.69E-46
MS.gene057208	MS.gene30920	0.821198	4.59E-53	-1.69E-46
MS.gene057414	MS.gene30920	0.811161	8.04E-51	-1.69E-46
MS.gene057444	MS.gene30920	0.859134	5.10E-63	-1.69E-46
MS.gene057598	MS.gene30920	0.804191	2.43E-49	-1.69E-46
MS.gene057693	MS.gene30920	0.835962	1.24E-56	-1.69E-46
MS.gene057705	MS.gene30920	0.80293	4.44E-49	-1.69E-46
MS.gene058037	MS.gene30920	0.820092	8.23E-53	-1.69E-46
MS.gene058084	MS.gene30920	0.808827	2.56E-50	-1.69E-46
MS.gene05847	MS.gene30920	0.819308	1.24E-52	-1.69E-46
MS.gene058548	MS.gene30920	0.813235	2.84E-51	-1.69E-46
MS.gene058549	MS.gene30920	0.800206	1.61E-48	-1.69E-46
MS.gene058625	MS.gene30920	0.845727	3.39E-59	-1.69E-46
MS.gene058636	MS.gene30920	0.802482	5.49E-49	-1.69E-46
MS.gene058783	MS.gene30920	0.811259	7.66E-51	-1.69E-46
MS.gene058829	MS.gene30920	0.807421	5.10E-50	-1.69E-46
MS.gene058979	MS.gene30920	0.829514	4.93E-55	-1.69E-46
MS.gene059084	MS.gene30920	0.8086	2.86E-50	-1.69E-46
MS.gene059151	MS.gene30920	0.811549	6.62E-51	-1.69E-46
MS.gene05950	MS.gene30920	0.808233	3.43E-50	-1.69E-46
MS.gene05954	MS.gene30920	0.831979	1.23E-55	-1.69E-46
MS.gene059625	MS.gene30920	0.841905	3.58E-58	-1.69E-46
MS.gene05979	MS.gene30920	0.816504	5.34E-52	-1.69E-46
MS.gene059842	MS.gene30920	0.819949	8.88E-53	-1.69E-46
MS.gene059935	MS.gene30920	0.812677	3.76E-51	-1.69E-46
MS.gene060322	MS.gene30920	0.809705	1.66E-50	-1.69E-46
MS.gene060365	MS.gene30920	0.832716	8.07E-56	-1.69E-46
MS.gene060429	MS.gene30920	0.869848	2.28E-66	-1.69E-46
MS.gene060521	MS.gene30920	0.813617	2.34E-51	-1.69E-46
MS.gene060572	MS.gene30920	0.863049	3.29E-64	-1.69E-46
MS.gene060587	MS.gene30920	0.813762	2.17E-51	-1.69E-46
MS.gene060634	MS.gene30920	-0.801251	9.83E-49	-1.69E-46
MS.gene060803	MS.gene30920	0.825103	5.63E-54	-1.69E-46
MS.gene060907	MS.gene30920	0.827884	1.22E-54	-1.69E-46
MS.gene060917	MS.gene30920	0.807848	4.14E-50	-1.69E-46
MS.gene061040	MS.gene30920	-0.800197	1.61E-48	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene30920	MS.gene04452	PPI
MS.gene30920	MS.gene070244	PPI
MS.gene30920	MS.gene004121	PPI
MS.gene060463	MS.gene30920	PPI
MS.gene30920	MS.gene009027	PPI
MS.gene30920	MS.gene038469	PPI
MS.gene30920	MS.gene061867	PPI
MS.gene30920	MS.gene04064	PPI
MS.gene30920	MS.gene008276	PPI
MS.gene30920	MS.gene05619	PPI
MS.gene30920	MS.gene73136	PPI
MS.gene061872	MS.gene30920	PPI
MS.gene30920	MS.gene049821	PPI
MS.gene30920	MS.gene04068	PPI
MS.gene30920	MS.gene022946	PPI
MS.gene30920	MS.gene035719	PPI
MS.gene30920	MS.gene05623	PPI
MS.gene30920	MS.gene061872	PPI
MS.gene30920	MS.gene89488	PPI
MS.gene070241	MS.gene30920	PPI
MS.gene30920	MS.gene66225	PPI
MS.gene30920	MS.gene035720	PPI
MS.gene30920	MS.gene70955	PPI
MS.gene30920	MS.gene61017	PPI
MS.gene30920	MS.gene04576	PPI
MS.gene30920	MS.gene09871	PPI
MS.gene061876	MS.gene30920	PPI
MS.gene30920	MS.gene012390	PPI
MS.gene30920	MS.gene08121	PPI
MS.gene30920	MS.gene64920	PPI
MS.gene012391	MS.gene30920	PPI
MS.gene30920	MS.gene064557	PPI
MS.gene070244	MS.gene30920	PPI
MS.gene30920	MS.gene04434	PPI
MS.gene88680	MS.gene30920	PPI
MS.gene30920	MS.gene04392	PPI
MS.gene30920	MS.gene89487	PPI
MS.gene30920	MS.gene010219	PPI
MS.gene30920	MS.gene04433	PPI
MS.gene30920	MS.gene040383	PPI
MS.gene009027	MS.gene30920	PPI
MS.gene30920	MS.gene68545	PPI
MS.gene31196	MS.gene30920	PPI
MS.gene30920	MS.gene070242	PPI
MS.gene30920	MS.gene016624	PPI
MS.gene060465	MS.gene30920	PPI
MS.gene30920	MS.gene048746	PPI
MS.gene30920	MS.gene028629	PPI
MS.gene30920	MS.gene58854	PPI
MS.gene30920	MS.gene043967	PPI
MS.gene30920	MS.gene31196	PPI
MS.gene061870	MS.gene30920	PPI
MS.gene30920	MS.gene060465	PPI
MS.gene30920	MS.gene035897	PPI
MS.gene30920	MS.gene049705	PPI
MS.gene30920	MS.gene061870	PPI
MS.gene035718	MS.gene30920	PPI
MS.gene30920	MS.gene070907	PPI
MS.gene30920	MS.gene05608	PPI
MS.gene78578	MS.gene30920	PPI
MS.gene30920	MS.gene88681	PPI
MS.gene30920	MS.gene82546	PPI
MS.gene30920	MS.gene60093	PPI
MS.gene028629	MS.gene30920	PPI
MS.gene30920	MS.gene08122	PPI
MS.gene30920	MS.gene09873	PPI
MS.gene30920	MS.gene035718	PPI
MS.gene30920	MS.gene022932	PPI
MS.gene035787	MS.gene30920	PPI
MS.gene04067	MS.gene30920	PPI
MS.gene71215	MS.gene30920	PPI
MS.gene30920	MS.gene010220	PPI
MS.gene30920	MS.gene04972	PPI
MS.gene30920	MS.gene62520	PPI
MS.gene30920	MS.gene08053	PPI
MS.gene30920	MS.gene018578	PPI
MS.gene30920	MS.gene035721	PPI
MS.gene30920	MS.gene018580	PPI
MS.gene30920	MS.gene004124	PPI
MS.gene071136	MS.gene30920	PPI
MS.gene30920	MS.gene88680	PPI
MS.gene30920	MS.gene072493	PPI
MS.gene30920	MS.gene04052	PPI
MS.gene30920	MS.gene84536	PPI
MS.gene30920	MS.gene035896	PPI
MS.gene30920	MS.gene061866	PPI
MS.gene056215	MS.gene30920	PPI
MS.gene30920	MS.gene64650	PPI
MS.gene30920	MS.gene79195	PPI
MS.gene30920	MS.gene048705	PPI
MS.gene30920	MS.gene061874	PPI
MS.gene30920	MS.gene061869	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30920.t1	MTR_7g029510	100.000	103	0	1	103	1	103	6.13e-69	204
MS.gene30920.t1	MTR_7g099610	100.000	103	0	1	103	1	103	6.13e-69	204
MS.gene30920.t1	MTR_5g063620	100.000	103	0	1	103	1	103	6.13e-69	204
MS.gene30920.t1	MTR_4g061827	100.000	103	0	1	103	1	103	6.13e-69	204
MS.gene30920.t1	MTR_4g061920	100.000	103	0	1	103	1	103	6.13e-69	204
MS.gene30920.t1	MTR_3g462400	100.000	103	0	1	103	1	103	6.13e-69	204
MS.gene30920.t1	MTR_3g054380	100.000	103	0	1	103	1	103	6.13e-69	204
MS.gene30920.t1	MTR_3g462730	100.000	103	0	1	103	1	103	6.13e-69	204
MS.gene30920.t1	MTR_3g462510	100.000	103	0	1	103	1	103	6.13e-69	204
MS.gene30920.t1	MTR_3g070920	100.000	103	0	1	103	1	103	6.13e-69	204
MS.gene30920.t1	MTR_3g061440	100.000	103	0	1	103	1	103	6.13e-69	204
MS.gene30920.t1	MTR_3g462410	100.000	103	0	1	103	1	103	6.13e-69	204
MS.gene30920.t1	MTR_2g096100	100.000	103	0	1	103	1	103	6.13e-69	204
MS.gene30920.t1	MTR_8g038460	100.000	103	0	1	103	1	103	6.13e-69	204
MS.gene30920.t1	MTR_4g128150	100.000	102	0	1	102	1	102	5.01e-66	207
MS.gene30920.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.09e-20	87.4

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30920.t1	AT5G59970	100.000	103	0	1	103	1	103	6.42e-69	204
MS.gene30920.t1	AT5G59690	100.000	103	0	1	103	1	103	6.42e-69	204
MS.gene30920.t1	AT5G59970	100.000	103	0	1	103	1	103	6.42e-69	204
MS.gene30920.t1	AT3G46320	100.000	103	0	1	103	1	103	6.42e-69	204
MS.gene30920.t1	AT3G45930	100.000	103	0	1	103	1	103	6.42e-69	204
MS.gene30920.t1	AT3G53730	100.000	103	0	1	103	1	103	6.42e-69	204
MS.gene30920.t1	AT2G28740	100.000	103	0	1	103	1	103	6.42e-69	204
MS.gene30920.t1	AT1G07660	100.000	103	0	1	103	1	103	6.42e-69	204
MS.gene30920.t1	AT1G07820	100.000	103	0	1	103	1	103	6.42e-69	204
MS.gene30920.t1	AT1G07820	100.000	103	0	1	103	1	103	6.42e-69	204
MS.gene30920.t1	AT1G07660	83.495	103	1	1	103	1	86	1.59e-51	160

Find 45 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGACAAGGAAGGACTCTTTA+TGG	0.217305	2.2:-5829142	MS.gene30920:CDS
TTGCGATGGTATTACTTATT+GGG	0.261353	2.2:-5828146	MS.gene30920:CDS
GGAAGGACTCTTTATGGATT+TGG	0.266178	2.2:-5829136	MS.gene30920:CDS
GTTGCGATGGTATTACTTAT+TGG	0.321655	2.2:-5828147	MS.gene30920:CDS
GAAGGACTCTTTATGGATTT+GGG	0.322097	2.2:-5829135	MS.gene30920:CDS
TGGAAAAGGTGGAAAGGGTT+TGG	0.322287	2.2:-5829407	MS.gene30920:CDS
GGAAAAGGTGGAAAGGGTTT+GGG	0.363863	2.2:-5829406	MS.gene30920:CDS
GAAGCCTGCGATTCGTCGTT+TGG	0.391757	2.2:-5829326	MS.gene30920:CDS
GGAAAGGGTTTGGGAAAGGG+AGG	0.393661	2.2:-5829397	MS.gene30920:CDS
TTGTTGCGAATCAAGATGTC+TGG	0.418777	2.2:-5829433	None:intergenic
TTGTTCAGCTATAAATTTCA+CGG	0.423417	2.2:-5827843	MS.gene30920:CDS
TTTGCGAAGGTTGATCGAAT+AGG	0.428662	2.2:+5827779	None:intergenic
GGTGGAAAGGGTTTGGGAAA+GGG	0.444952	2.2:-5829400	MS.gene30920:CDS
TGGTATTACTTATTGGGGTA+TGG	0.468441	2.2:-5828140	MS.gene30920:CDS
AGGTGGAAAGGGTTTGGGAA+AGG	0.475381	2.2:-5829401	MS.gene30920:CDS
AAGATGTCTGGACGTGGAAA+AGG	0.490201	2.2:-5829421	MS.gene30920:CDS
ATGCAGTAGTGTGGTACTCT+TGG	0.490797	2.2:-5828104	MS.gene30920:CDS
GTGCTTCGTGATAACATTCA+AGG	0.499787	2.2:-5829355	MS.gene30920:CDS
AATTGAATGTACGATAAGAA+AGG	0.506331	2.2:+5827756	None:intergenic
GAGAAAGACTGTTACTGCTA+TGG	0.515154	2.2:-5829185	MS.gene30920:CDS
TGTTATCACGAAGCACCTTA+CGG	0.542642	2.2:+5829362	None:intergenic
CGAATCAAGATGTCTGGACG+TGG	0.544605	2.2:-5829427	None:intergenic
TGCGATGGTATTACTTATTG+GGG	0.547304	2.2:-5828145	MS.gene30920:CDS
TTACTTACACAGAGCACGCT+AGG	0.553235	2.2:-5829207	MS.gene30920:CDS
CGTCGTTTGGCACGTAGAGG+AGG	0.553789	2.2:-5829313	MS.gene30920:CDS
TCTCAACTTACAGAAAGAGT+AGG	0.556129	2.2:+5828052	None:intergenic
GGACGTGGAAAAGGTGGAAA+GGG	0.568194	2.2:-5829412	MS.gene30920:CDS
AGGAGCAAAACGACACCGTA+AGG	0.575994	2.2:-5829377	MS.gene30920:CDS
AGAGTACCACACTACTGCAT+TGG	0.579336	2.2:+5828107	None:intergenic
CGTGCCAAACGACGAATCGC+AGG	0.582056	2.2:+5829322	None:intergenic
GGAGAATGTTATTCGTGATG+CGG	0.582811	2.2:-5829230	MS.gene30920:CDS
ATGTCTGGACGTGGAAAAGG+TGG	0.583297	2.2:-5829418	MS.gene30920:CDS
TGGACGTGGAAAAGGTGGAA+AGG	0.597841	2.2:-5829413	MS.gene30920:CDS
ACGATAAGAAAGGTTTGCGA+AGG	0.601081	2.2:+5827766	None:intergenic
AAGGACTCTTTATGGATTTG+GGG	0.603730	2.2:-5829134	MS.gene30920:CDS
ATTCGTCGTTTGGCACGTAG+AGG	0.619364	2.2:-5829316	MS.gene30920:CDS
GTGGATGCAGGATGTTGCGA+TGG	0.626932	2.2:-5828160	MS.gene30920:intron
GTTTATGCATTGAAGAGACA+AGG	0.629886	2.2:-5829157	MS.gene30920:CDS
GGAGGAGTGAAGCGTATAAG+CGG	0.630598	2.2:-5829295	MS.gene30920:CDS
ATGCATTGAAGAGACAAGGA+AGG	0.634793	2.2:-5829153	MS.gene30920:CDS
ACTCTTGGCTTGTGAAACAC+AGG	0.642689	2.2:-5828089	MS.gene30920:CDS
AGGACTCTTTATGGATTTGG+GGG	0.645439	2.2:-5829133	MS.gene30920:intron
AAAATTGAATATGCTAACAG+AGG	0.648063	2.2:+5827820	None:intergenic
TAAAGACCAATGCAGTAGTG+TGG	0.659395	2.2:-5828113	MS.gene30920:CDS
TTGATCTACGAAGAGACACG+TGG	0.703849	2.2:-5829271	MS.gene30920:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTATTATTATAAGTGATTA+TGG	-	chr2.2:5828100-5828119	MS.gene30920:CDS	10.0%
!!	TTATTATTATAAGTGATTAT+GGG	-	chr2.2:5828101-5828120	MS.gene30920:CDS	10.0%
!!	AATTTAATATTGAATAATGC+TGG	-	chr2.2:5828915-5828934	MS.gene30920:intron	15.0%
!!	AAAAATTGGAAATATTGCAT+TGG	+	chr2.2:5828646-5828665	None:intergenic	20.0%
!!	AACATTTCAATACTAAAGAT+TGG	+	chr2.2:5828540-5828559	None:intergenic	20.0%
!!!	ATTCAATATCATTTTGTCTA+TGG	-	chr2.2:5828295-5828314	MS.gene30920:intron	20.0%
!!!	TTTTTTTAGGGTAAGAATTT+AGG	-	chr2.2:5828184-5828203	MS.gene30920:intron	20.0%
!	AAAATTGAATATGCTAACAG+AGG	+	chr2.2:5829352-5829371	None:intergenic	25.0%
!	AAACTAAAACCTAAAACATC+AGG	+	chr2.2:5828582-5828601	None:intergenic	25.0%
!	AATACTCACAACAATTTGAT+AGG	+	chr2.2:5828453-5828472	None:intergenic	25.0%
!	AATTGAATGTACGATAAGAA+AGG	+	chr2.2:5829416-5829435	None:intergenic	25.0%
!	ACATCTTAAAATTCAATTGC+AGG	+	chr2.2:5828357-5828376	None:intergenic	25.0%
!	ATTATAAGTGATTATGGGTT+GGG	-	chr2.2:5828106-5828125	MS.gene30920:CDS	25.0%
!	ATTCCATATATGACAAATTG+AGG	+	chr2.2:5828501-5828520	None:intergenic	25.0%
!	TATTATAAGTGATTATGGGT+TGG	-	chr2.2:5828105-5828124	MS.gene30920:CDS	25.0%
!	TGCAGATTTCTTAGAAAAAT+TGG	+	chr2.2:5828660-5828679	None:intergenic	25.0%
!	TTCCATATATGACAAATTGA+GGG	+	chr2.2:5828500-5828519	None:intergenic	25.0%
!	TTGTTCAGCTATAAATTTCA+CGG	-	chr2.2:5829326-5829345	MS.gene30920:CDS	25.0%
!!	ATTTTCAAAGTCGTTGTTAA+CGG	-	chr2.2:5828381-5828400	MS.gene30920:intron	25.0%
!!	GAGTAATTATCTTTTGTGAT+AGG	-	chr2.2:5828206-5828225	MS.gene30920:intron	25.0%
!!!	AATTTAGGTGGTGTTTTTTT+AGG	-	chr2.2:5828171-5828190	MS.gene30920:intron	25.0%
!!!	ATTTAGGTGGTGTTTTTTTA+GGG	-	chr2.2:5828172-5828191	MS.gene30920:intron	25.0%
	AACCCTCAATTTGTCATATA+TGG	-	chr2.2:5828495-5828514	MS.gene30920:intron	30.0%
	AAGTTGAGAAAAGTGGAATT+TGG	-	chr2.2:5829131-5829150	MS.gene30920:intron	30.0%
	ACAATTTGATAGGCGATATT+AGG	+	chr2.2:5828443-5828462	None:intergenic	30.0%
	AGATTTCTAAATTCTCCAGA+TGG	+	chr2.2:5828840-5828859	None:intergenic	30.0%
	ATAGGTAGTTAACTGTTTCA+TGG	-	chr2.2:5828224-5828243	MS.gene30920:intron	30.0%
	ATCTGGAGAATTTAGAAATC+TGG	-	chr2.2:5828839-5828858	MS.gene30920:intron	30.0%
	ATTGATGAGGATGTTATTGT+GGG	-	chr2.2:5828709-5828728	MS.gene30920:intron	30.0%
	CAAATACTATGTGATTGATG+AGG	-	chr2.2:5828696-5828715	MS.gene30920:intron	30.0%
	TCACAACTGAAGATGTTTAA+AGG	+	chr2.2:5828737-5828756	None:intergenic	30.0%
	TCTGGAGAATTTAGAAATCT+GGG	-	chr2.2:5828840-5828859	MS.gene30920:intron	30.0%
	TTAAAATTCAATTGCAGGCT+TGG	+	chr2.2:5828352-5828371	None:intergenic	30.0%
	TTGCGATGGTATTACTTATT+GGG	-	chr2.2:5829023-5829042	MS.gene30920:intron	30.0%
!	ACAAATTTTCTGTGTAAGAG+AGG	+	chr2.2:5829310-5829329	None:intergenic	30.0%
!	AGTTATGACCAACTAGATTT+TGG	-	chr2.2:5828762-5828781	MS.gene30920:intron	30.0%
!	ATTGGAAATATTGCATTGGT+TGG	+	chr2.2:5828642-5828661	None:intergenic	30.0%
!	GTTATGACCAACTAGATTTT+GGG	-	chr2.2:5828763-5828782	MS.gene30920:intron	30.0%
!	TGTTTGGAATTATGTCTTTG+TGG	-	chr2.2:5828990-5829009	MS.gene30920:intron	30.0%
!	TTAAGTTGTCTTCTTGAAGT+AGG	-	chr2.2:5828863-5828882	MS.gene30920:intron	30.0%
!	TTTCTGTAAGTTGAGAAAAG+TGG	-	chr2.2:5829124-5829143	MS.gene30920:intron	30.0%
	AAGGACTCTTTATGGATTTG+GGG	-	chr2.2:5828035-5828054	MS.gene30920:intron	35.0%
	AATTATGTCTTTGTGGATGC+AGG	-	chr2.2:5828997-5829016	MS.gene30920:intron	35.0%
	ACATAATTCCAAACAGACAG+GGG	+	chr2.2:5828985-5829004	None:intergenic	35.0%
	AGAATATGATGAGGATACTG+AGG	+	chr2.2:5829282-5829301	None:intergenic	35.0%
	AGACATAATTCCAAACAGAC+AGG	+	chr2.2:5828987-5829006	None:intergenic	35.0%
	CAAGGTTTGTGGTTGAATTT+AGG	-	chr2.2:5828156-5828175	MS.gene30920:intron	35.0%
	CTGCAGTAAATTGATCCAAT+GGG	+	chr2.2:5828949-5828968	None:intergenic	35.0%
	GAAGGACTCTTTATGGATTT+GGG	-	chr2.2:5828034-5828053	MS.gene30920:intron	35.0%
	GACATAATTCCAAACAGACA+GGG	+	chr2.2:5828986-5829005	None:intergenic	35.0%
	GATTGATGAGGATGTTATTG+TGG	-	chr2.2:5828708-5828727	MS.gene30920:intron	35.0%
	GTTGCGATGGTATTACTTAT+TGG	-	chr2.2:5829022-5829041	MS.gene30920:intron	35.0%
	GTTTATGCATTGAAGAGACA+AGG	-	chr2.2:5828012-5828031	MS.gene30920:intron	35.0%
	TCTCAACTTACAGAAAGAGT+AGG	+	chr2.2:5829120-5829139	None:intergenic	35.0%
	TCTCCAGATGGAAAAACATA+CGG	+	chr2.2:5828828-5828847	None:intergenic	35.0%
	TGCGATGGTATTACTTATTG+GGG	-	chr2.2:5829024-5829043	MS.gene30920:intron	35.0%
	TGGTATTACTTATTGGGGTA+TGG	-	chr2.2:5829029-5829048	MS.gene30920:intron	35.0%
!	AACGTGTGAGTTTTTCTACT+AGG	-	chr2.2:5829209-5829228	MS.gene30920:CDS	35.0%
!!	AGAAATTTTGGCAAGGTTTG+TGG	-	chr2.2:5828145-5828164	MS.gene30920:CDS	35.0%
!!	GTCTTGCTGTCAAGAAATTT+TGG	-	chr2.2:5828133-5828152	MS.gene30920:CDS	35.0%
	ACGATAAGAAAGGTTTGCGA+AGG	+	chr2.2:5829406-5829425	None:intergenic	40.0%
	AGACAAGGAAGGACTCTTTA+TGG	-	chr2.2:5828027-5828046	MS.gene30920:intron	40.0%
	AGGACTCTTTATGGATTTGG+GGG	-	chr2.2:5828036-5828055	MS.gene30920:intron	40.0%
	AGTTGTCTTCTTGAAGTAGG+TGG	-	chr2.2:5828866-5828885	MS.gene30920:intron	40.0%
	ATGCATTGAAGAGACAAGGA+AGG	-	chr2.2:5828016-5828035	MS.gene30920:intron	40.0%
	ATGCTGGAAAACTATCCCAT+TGG	-	chr2.2:5828931-5828950	MS.gene30920:intron	40.0%
	CTCCAGATGGAAAAACATAC+GGG	+	chr2.2:5828827-5828846	None:intergenic	40.0%
	CTTTATGGATTTGGGGGTTA+GGG	-	chr2.2:5828042-5828061	MS.gene30920:intron	40.0%
	GAGAAAGACTGTTACTGCTA+TGG	-	chr2.2:5827984-5828003	MS.gene30920:intron	40.0%
	GCTGCAGTAAATTGATCCAA+TGG	+	chr2.2:5828950-5828969	None:intergenic	40.0%
	GGAAGGACTCTTTATGGATT+TGG	-	chr2.2:5828033-5828052	MS.gene30920:intron	40.0%
	GGAGAATGTTATTCGTGATG+CGG	-	chr2.2:5827939-5827958	MS.gene30920:intron	40.0%
	GGTTTGTGGTTGAATTTAGG+TGG	-	chr2.2:5828159-5828178	MS.gene30920:intron	40.0%
	GTGCTTCGTGATAACATTCA+AGG	-	chr2.2:5827814-5827833	MS.gene30920:CDS	40.0%
	TAAAGACCAATGCAGTAGTG+TGG	-	chr2.2:5829056-5829075	MS.gene30920:intron	40.0%
	TCTTTATGGATTTGGGGGTT+AGG	-	chr2.2:5828041-5828060	MS.gene30920:intron	40.0%
	TTGATAGGCGATATTAGGCT+TGG	+	chr2.2:5828438-5828457	None:intergenic	40.0%
!	ATCCCGTATGTTTTTCCATC+TGG	-	chr2.2:5828822-5828841	MS.gene30920:intron	40.0%
!	ATTTTCTGTGTAAGAGAGGC+AGG	+	chr2.2:5829306-5829325	None:intergenic	40.0%
!	CTGTCAACTCCTGATGTTTT+AGG	-	chr2.2:5828570-5828589	MS.gene30920:intron	40.0%
!	TGGCGTGTTGAAGATCTTTT+TGG	-	chr2.2:5827918-5827937	MS.gene30920:intron	40.0%
!	TGTTATCACGAAGCACCTTA+CGG	+	chr2.2:5827810-5827829	None:intergenic	40.0%
!	TTTGCGAAGGTTGATCGAAT+AGG	+	chr2.2:5829393-5829412	None:intergenic	40.0%
!!	AAGAGCACCCAAAATCTAGT+TGG	+	chr2.2:5828773-5828792	None:intergenic	40.0%
!!	GCTGTCAAGAAATTTTGGCA+AGG	-	chr2.2:5828138-5828157	MS.gene30920:CDS	40.0%
	ATGCAGTAGTGTGGTACTCT+TGG	-	chr2.2:5829065-5829084	MS.gene30920:intron	45.0%
	GGAAAAGGTGGAAAGGGTTT+GGG	-	chr2.2:5827763-5827782	MS.gene30920:CDS	45.0%
	GGAATTTGGAGAAATGCTGC+AGG	-	chr2.2:5829145-5829164	MS.gene30920:CDS	45.0%
	TGGAAAAGGTGGAAAGGGTT+TGG	-	chr2.2:5827762-5827781	MS.gene30920:CDS	45.0%
	TTACTTACACAGAGCACGCT+AGG	-	chr2.2:5827962-5827981	MS.gene30920:intron	45.0%
	TTGATCTACGAAGAGACACG+TGG	-	chr2.2:5827898-5827917	MS.gene30920:intron	45.0%
!	AGAGTACCACACTACTGCAT+TGG	+	chr2.2:5829065-5829084	None:intergenic	45.0%
!!	ACTCTTGGCTTGTGAAACAC+AGG	-	chr2.2:5829080-5829099	MS.gene30920:intron	45.0%
	AGGAGCAAAACGACACCGTA+AGG	-	chr2.2:5827792-5827811	MS.gene30920:CDS	50.0%
	AGGTGGAAAGGGTTTGGGAA+AGG	-	chr2.2:5827768-5827787	MS.gene30920:CDS	50.0%
	ATGTCTGGACGTGGAAAAGG+TGG	-	chr2.2:5827751-5827770	MS.gene30920:CDS	50.0%
	ATTCGTCGTTTGGCACGTAG+AGG	-	chr2.2:5827853-5827872	MS.gene30920:intron	50.0%
	GAGGCAGGCAGAATATGATG+AGG	+	chr2.2:5829291-5829310	None:intergenic	50.0%
	GGACGTGGAAAAGGTGGAAA+GGG	-	chr2.2:5827757-5827776	MS.gene30920:CDS	50.0%
	GGAGGAGTGAAGCGTATAAG+CGG	-	chr2.2:5827874-5827893	MS.gene30920:intron	50.0%
	GGTGGAAAGGGTTTGGGAAA+GGG	-	chr2.2:5827769-5827788	MS.gene30920:CDS	50.0%
	GTCGTTGTTAACGGCTTCTC+TGG	-	chr2.2:5828390-5828409	MS.gene30920:intron	50.0%
	TCCAAACAGACAGGGGTTTC+TGG	+	chr2.2:5828978-5828997	None:intergenic	50.0%
	TCCAGAAACCCCTGTCTGTT+TGG	-	chr2.2:5828974-5828993	MS.gene30920:intron	50.0%
	TGGACGTGGAAAAGGTGGAA+AGG	-	chr2.2:5827756-5827775	MS.gene30920:CDS	50.0%
	GAAGCCTGCGATTCGTCGTT+TGG	-	chr2.2:5827843-5827862	MS.gene30920:CDS	55.0%
	GGAAAGGGTTTGGGAAAGGG+AGG	-	chr2.2:5827772-5827791	MS.gene30920:CDS	55.0%
	GTGGATGCAGGATGTTGCGA+TGG	-	chr2.2:5829009-5829028	MS.gene30920:intron	55.0%
	CGTCGTTTGGCACGTAGAGG+AGG	-	chr2.2:5827856-5827875	MS.gene30920:intron	60.0%
	CGTGCCAAACGACGAATCGC+AGG	+	chr2.2:5827850-5827869	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	5827751	5829440	5827751	ID=MS.gene30920
chr2.2	mRNA	5827751	5829440	5827751	ID=MS.gene30920.t1;Parent=MS.gene30920
chr2.2	exon	5829134	5829440	5829134	ID=MS.gene30920.t1.exon1;Parent=MS.gene30920.t1
chr2.2	CDS	5829134	5829440	5829134	ID=cds.MS.gene30920.t1;Parent=MS.gene30920.t1
chr2.2	exon	5828063	5828172	5828063	ID=MS.gene30920.t1.exon2;Parent=MS.gene30920.t1
chr2.2	CDS	5828063	5828172	5828063	ID=cds.MS.gene30920.t1;Parent=MS.gene30920.t1
chr2.2	exon	5827751	5827870	5827751	ID=MS.gene30920.t1.exon3;Parent=MS.gene30920.t1
chr2.2	CDS	5827751	5827870	5827751	ID=cds.MS.gene30920.t1;Parent=MS.gene30920.t1

Gene Sequence

>MS.gene30920

ATGTCTGGACGTGGAAAAGGTGGAAAGGGTTTGGGAAAGGGAGGAGCAAAACGACACCGTAAGGTGCTTCGTGATAACATTCAAGGAATAACGAAGCCTGCGATTCGTCGTTTGGCACGTAGAGGAGGAGTGAAGCGTATAAGCGGTTTGATCTACGAAGAGACACGTGGCGTGTTGAAGATCTTTTTGGAGAATGTTATTCGTGATGCGGTTACTTACACAGAGCACGCTAGGAGAAAGACTGTTACTGCTATGGATGTTGTTTATGCATTGAAGAGACAAGGAAGGACTCTTTATGGATTTGGGGGTTAGGGTTTGTGATTTTATAATGTTTCGTAGTTGTTTTTTTATTATTATTATAAGTGATTATGGGTTGGGAAAAGTCTTGCTGTCAAGAAATTTTGGCAAGGTTTGTGGTTGAATTTAGGTGGTGTTTTTTTAGGGTAAGAATTTAGGAGTAATTATCTTTTGTGATAGGTAGTTAACTGTTTCATGGTTTGAATCTGTTATGTAACTACTAGTTTTAATGAACAAGTGTTTCTGCATTCAATATCATTTTGTCTATGGTCTATTTAGTTTCTTATACTGATCTAATATTCCAAGCCTGCAATTGAATTTTAAGATGTTGAAATTTTCAAAGTCGTTGTTAACGGCTTCTCTGGTTTCTTATACTGATCTAATATTCCAAGCCTAATATCGCCTATCAAATTGTTGTGAGTATTTGATTAGAAGAATCTTACTGAAAACCCTCAATTTGTCATATATGGAATTGAATCTATACTGTAACCAATCTTTAGTATTGAAATGTTGTGTTGAACTCTGTCAACTCCTGATGTTTTAGGTTTTAGTTTTTGATTTGAAACTGTTCGTGTATTACAATTTCACTGTCCAACCAATGCAATATTTCCAATTTTTCTAAGAAATCTGCAATATAATTTTGAATAACAAATACTATGTGATTGATGAGGATGTTATTGTGGGTGCCTTTAAACATCTTCAGTTGTGAAATTGAGTTATGACCAACTAGATTTTGGGTGCTCTTGTACTTCAATCTGTACTCGTGTAATGTGTATCCCGTATGTTTTTCCATCTGGAGAATTTAGAAATCTGGGTTAAGTTGTCTTCTTGAAGTAGGTGGTGAAGAAACTTGAGATTTCGTTTAAGAATTTAATATTGAATAATGCTGGAAAACTATCCCATTGGATCAATTTACTGCAGCATGATCCAGAAACCCCTGTCTGTTTGGAATTATGTCTTTGTGGATGCAGGATGTTGCGATGGTATTACTTATTGGGGTATGGTCATTAAAGACCAATGCAGTAGTGTGGTACTCTTGGCTTGTGAAACACAGGAAATTTGTGTTGATCCTACTCTTTCTGTAAGTTGAGAAAAGTGGAATTTGGAGAAATGCTGCAGGTATATTTTATAGAACTTGCAATACTTCACTTCGTTGCACTTAACGTGTGAGTTTTTCTACTAGGTTCTTTAGAAATAAAAATAATTATATAGTCTTGCATATAGTTCATAGCCTCAGTATCCTCATCATATTCTGCCTGCCTCTCTTACACAGAAAATTTGTTCAGCTATAAATTTCACGGCCTCTGTTAGCATATTCAATTTTGTCAATAACAAAAATATTCCTATTCGATCAACCTTCGCAAACCTTTCTTATCGTACATTCAATTTGTAA

Protein sequence

>MS.gene30920.t1

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGGCCDGITYWGMVIKDQCSSVVLLACETQEICVDPTLSKICSAINFTASVSIFNFVNNKNIPIRSTFANLSYRTFNL