AlfalfaGEDB | MS.gene60093

M. sativa cultivar XinJiangDaYe / MS.gene60093

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60093.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60093.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60093.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene60093	0.817139	3.85E-52	-1.69E-46
MS.gene049411	MS.gene60093	0.806805	6.88E-50	-1.69E-46
MS.gene049821	MS.gene60093	0.870608	1.28E-66	-1.69E-46
MS.gene050277	MS.gene60093	0.810774	9.75E-51	-1.69E-46
MS.gene050689	MS.gene60093	0.81351	2.47E-51	-1.69E-46
MS.gene050752	MS.gene60093	0.837446	5.17E-57	-1.69E-46
MS.gene050838	MS.gene60093	0.865089	7.60E-65	-1.69E-46
MS.gene050901	MS.gene60093	0.810413	1.17E-50	-1.69E-46
MS.gene050955	MS.gene60093	0.820432	6.88E-53	-1.69E-46
MS.gene051003	MS.gene60093	0.831896	1.29E-55	-1.69E-46
MS.gene051233	MS.gene60093	0.817678	2.91E-52	-1.69E-46
MS.gene051234	MS.gene60093	0.829609	4.68E-55	-1.69E-46
MS.gene051328	MS.gene60093	0.826341	2.86E-54	-1.69E-46
MS.gene051330	MS.gene60093	0.817098	3.93E-52	-1.69E-46
MS.gene051343	MS.gene60093	0.849532	3.04E-60	-1.69E-46
MS.gene05134	MS.gene60093	0.828393	9.22E-55	-1.69E-46
MS.gene051676	MS.gene60093	0.848241	6.95E-60	-1.69E-46
MS.gene051700	MS.gene60093	0.820791	5.69E-53	-1.69E-46
MS.gene05188	MS.gene60093	0.832592	8.66E-56	-1.69E-46
MS.gene051902	MS.gene60093	0.852011	6.11E-61	-1.69E-46
MS.gene051903	MS.gene60093	0.827522	1.49E-54	-1.69E-46
MS.gene051975	MS.gene60093	0.846037	2.79E-59	-1.69E-46
MS.gene052133	MS.gene60093	0.816065	6.70E-52	-1.69E-46
MS.gene052335	MS.gene60093	0.810298	1.24E-50	-1.69E-46
MS.gene052479	MS.gene60093	0.808409	3.14E-50	-1.69E-46
MS.gene052947	MS.gene60093	0.875607	2.70E-68	-1.69E-46
MS.gene053058	MS.gene60093	0.826415	2.75E-54	-1.69E-46
MS.gene053563	MS.gene60093	0.801777	7.67E-49	-1.69E-46
MS.gene053564	MS.gene60093	0.841911	3.56E-58	-1.69E-46
MS.gene053581	MS.gene60093	0.824362	8.41E-54	-1.69E-46
MS.gene05364	MS.gene60093	0.819448	1.16E-52	-1.69E-46
MS.gene053952	MS.gene60093	0.813623	2.33E-51	-1.69E-46
MS.gene054403	MS.gene60093	0.839376	1.64E-57	-1.69E-46
MS.gene05469	MS.gene60093	0.828699	7.78E-55	-1.69E-46
MS.gene05517	MS.gene60093	0.808512	2.99E-50	-1.69E-46
MS.gene055311	MS.gene60093	0.820695	5.99E-53	-1.69E-46
MS.gene055313	MS.gene60093	0.815955	7.09E-52	-1.69E-46
MS.gene055314	MS.gene60093	0.80541	1.35E-49	-1.69E-46
MS.gene055468	MS.gene60093	0.810367	1.19E-50	-1.69E-46
MS.gene055513	MS.gene60093	0.804553	2.04E-49	-1.69E-46
MS.gene055555	MS.gene60093	0.819147	1.35E-52	-1.69E-46
MS.gene05608	MS.gene60093	0.809876	1.52E-50	-1.69E-46
MS.gene056215	MS.gene60093	0.858113	1.03E-62	-1.69E-46
MS.gene056580	MS.gene60093	0.814678	1.36E-51	-1.69E-46
MS.gene056631	MS.gene60093	0.827487	1.52E-54	-1.69E-46
MS.gene05689	MS.gene60093	0.802177	6.35E-49	-1.69E-46
MS.gene056986	MS.gene60093	0.831138	1.98E-55	-1.69E-46
MS.gene057094	MS.gene60093	0.803039	4.22E-49	-1.69E-46
MS.gene057102	MS.gene60093	0.804952	1.69E-49	-1.69E-46
MS.gene057694	MS.gene60093	0.849797	2.57E-60	-1.69E-46
MS.gene057705	MS.gene60093	0.863191	2.97E-64	-1.69E-46
MS.gene05781	MS.gene60093	0.808681	2.75E-50	-1.69E-46
MS.gene057890	MS.gene60093	-0.806636	7.47E-50	-1.69E-46
MS.gene058074	MS.gene60093	0.801429	9.04E-49	-1.69E-46
MS.gene058238	MS.gene60093	0.807395	5.16E-50	-1.69E-46
MS.gene058261	MS.gene60093	0.812144	4.91E-51	-1.69E-46
MS.gene058852	MS.gene60093	0.814207	1.73E-51	-1.69E-46
MS.gene059146	MS.gene60093	0.838206	3.30E-57	-1.69E-46
MS.gene059171	MS.gene60093	0.801241	9.88E-49	-1.69E-46
MS.gene059227	MS.gene60093	0.837072	6.45E-57	-1.69E-46
MS.gene06001	MS.gene60093	0.837018	6.66E-57	-1.69E-46
MS.gene06011	MS.gene60093	0.812416	4.29E-51	-1.69E-46
MS.gene060365	MS.gene60093	0.831298	1.81E-55	-1.69E-46
MS.gene060429	MS.gene60093	0.845003	5.32E-59	-1.69E-46
MS.gene060917	MS.gene60093	0.808251	3.39E-50	-1.69E-46
MS.gene061100	MS.gene60093	0.816516	5.31E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene04434	MS.gene60093	PPI
MS.gene60093	MS.gene018580	PPI
MS.gene60093	MS.gene004124	PPI
MS.gene08121	MS.gene60093	PPI
MS.gene022932	MS.gene60093	PPI
MS.gene88681	MS.gene60093	PPI
MS.gene035718	MS.gene60093	PPI
MS.gene035896	MS.gene60093	PPI
MS.gene73136	MS.gene60093	PPI
MS.gene008276	MS.gene60093	PPI
MS.gene60093	MS.gene64650	PPI
MS.gene070907	MS.gene60093	PPI
MS.gene60093	MS.gene061874	PPI
MS.gene004124	MS.gene60093	PPI
MS.gene009027	MS.gene60093	PPI
MS.gene018578	MS.gene60093	PPI
MS.gene010219	MS.gene60093	PPI
MS.gene04052	MS.gene60093	PPI
MS.gene08122	MS.gene60093	PPI
MS.gene060465	MS.gene60093	PPI
MS.gene048705	MS.gene60093	PPI
MS.gene05623	MS.gene60093	PPI
MS.gene043967	MS.gene60093	PPI
MS.gene60093	MS.gene04972	PPI
MS.gene60093	MS.gene004121	PPI
MS.gene84536	MS.gene60093	PPI
MS.gene04972	MS.gene60093	PPI
MS.gene028629	MS.gene60093	PPI
MS.gene04067	MS.gene60093	PPI
MS.gene71215	MS.gene60093	PPI
MS.gene061866	MS.gene60093	PPI
MS.gene061870	MS.gene60093	PPI
MS.gene004121	MS.gene60093	PPI
MS.gene60093	MS.gene89488	PPI
MS.gene66225	MS.gene60093	PPI
MS.gene071136	MS.gene60093	PPI
MS.gene012390	MS.gene60093	PPI
MS.gene09873	MS.gene60093	PPI
MS.gene78578	MS.gene60093	PPI
MS.gene79195	MS.gene60093	PPI
MS.gene035721	MS.gene60093	PPI
MS.gene056215	MS.gene60093	PPI
MS.gene064557	MS.gene60093	PPI
MS.gene038469	MS.gene60093	PPI
MS.gene82546	MS.gene60093	PPI
MS.gene61017	MS.gene60093	PPI
MS.gene04433	MS.gene60093	PPI
MS.gene04452	MS.gene60093	PPI
MS.gene60093	MS.gene016624	PPI
MS.gene64650	MS.gene60093	PPI
MS.gene035787	MS.gene60093	PPI
MS.gene061874	MS.gene60093	PPI
MS.gene89487	MS.gene60093	PPI
MS.gene035719	MS.gene60093	PPI
MS.gene58854	MS.gene60093	PPI
MS.gene016624	MS.gene60093	PPI
MS.gene70955	MS.gene60093	PPI
MS.gene040383	MS.gene60093	PPI
MS.gene070242	MS.gene60093	PPI
MS.gene04392	MS.gene60093	PPI
MS.gene072493	MS.gene60093	PPI
MS.gene035720	MS.gene60093	PPI
MS.gene30920	MS.gene60093	PPI
MS.gene048746	MS.gene60093	PPI
MS.gene010220	MS.gene60093	PPI
MS.gene08053	MS.gene60093	PPI
MS.gene061872	MS.gene60093	PPI
MS.gene60093	MS.gene049705	PPI
MS.gene88680	MS.gene60093	PPI
MS.gene68545	MS.gene60093	PPI
MS.gene070241	MS.gene60093	PPI
MS.gene061869	MS.gene60093	PPI
MS.gene049821	MS.gene60093	PPI
MS.gene022946	MS.gene60093	PPI
MS.gene62520	MS.gene60093	PPI
MS.gene018580	MS.gene60093	PPI
MS.gene04064	MS.gene60093	PPI
MS.gene60093	MS.gene68545	PPI
MS.gene061876	MS.gene60093	PPI
MS.gene05619	MS.gene60093	PPI
MS.gene05608	MS.gene60093	PPI
MS.gene012391	MS.gene60093	PPI
MS.gene049705	MS.gene60093	PPI
MS.gene070244	MS.gene60093	PPI
MS.gene09871	MS.gene60093	PPI
MS.gene035897	MS.gene60093	PPI
MS.gene64920	MS.gene60093	PPI
MS.gene04576	MS.gene60093	PPI
MS.gene060463	MS.gene60093	PPI
MS.gene31196	MS.gene60093	PPI
MS.gene89488	MS.gene60093	PPI
MS.gene04068	MS.gene60093	PPI
MS.gene061867	MS.gene60093	PPI
MS.gene60093	MS.gene018578	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60093.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene60093.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene60093.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene60093.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene60093.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene60093.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene60093.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene60093.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene60093.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene60093.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene60093.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene60093.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene60093.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene60093.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene60093.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene60093.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60093.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene60093.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene60093.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene60093.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene60093.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene60093.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene60093.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene60093.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene60093.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene60093.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene60093.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 28 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAAGGACTTTGTATGGTTT+CGG	0.343848	8.4:+59702915	MS.gene60093:CDS
TCGCTGGCTTTGTGATTCCT+TGG	0.395127	8.4:-59702713	None:intergenic
GGGAGGTAAGGGACTAGGAA+AGG	0.428277	8.4:+59702650	MS.gene60093:CDS
AGACAAGGAAGGACTTTGTA+TGG	0.433181	8.4:+59702909	MS.gene60093:CDS
CTCGCTAATCGACGAATCGC+TGG	0.450047	8.4:-59702729	None:intergenic
GGAGGTAAGGGACTAGGAAA+GGG	0.452874	8.4:+59702651	MS.gene60093:CDS
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	8.4:+59702638	MS.gene60093:CDS
GATTCTCCATTGAAAATGTC+AGG	0.496148	8.4:+59702618	None:intergenic
GAGGAAGACGGTGACTGCTA+TGG	0.517708	8.4:+59702866	MS.gene60093:CDS
GGAAAGGGAGGTAAGGGACT+AGG	0.521051	8.4:+59702645	MS.gene60093:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.538992	8.4:+59702630	None:intergenic
CCATTGAAAATGTCAGGTCG+TGG	0.548627	8.4:+59702624	None:intergenic
GGTAAGGGACTAGGAAAGGG+AGG	0.563878	8.4:+59702654	MS.gene60093:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	8.4:+59702639	MS.gene60093:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	8.4:+59702629	None:intergenic
CGTCGATTAGCGAGAAGAGG+AGG	0.585804	8.4:+59702738	MS.gene60093:CDS
AGGACTTTGTATGGTTTCGG+AGG	0.599906	8.4:+59702918	MS.gene60093:CDS
ATGCTTTGAAGAGACAAGGA+AGG	0.603293	8.4:+59702898	MS.gene60093:CDS
ATTCGTCGATTAGCGAGAAG+AGG	0.605618	8.4:+59702735	MS.gene60093:CDS
GTTCTTCGCGACAACATCCA+AGG	0.612538	8.4:+59702696	MS.gene60093:CDS
GGAGGTGTGAAGAGAATCAG+CGG	0.613587	8.4:+59702756	MS.gene60093:CDS
TGAACATGCGAGGAGGAAGA+CGG	0.616728	8.4:+59702854	MS.gene60093:CDS
GTTTATGCTTTGAAGAGACA+AGG	0.627217	8.4:+59702894	MS.gene60093:CDS
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	8.4:+59702633	MS.gene60093:CDS
GAGGTGTGAAGAGAATCAGC+GGG	0.664332	8.4:+59702757	MS.gene60093:CDS
CGTATACTGAACATGCGAGG+AGG	0.708048	8.4:+59702847	MS.gene60093:CDS
TTGATTTACGAAGAAACTCG+CGG	0.712840	8.4:+59702780	MS.gene60093:CDS
TTACGTATACTGAACATGCG+AGG	0.714123	8.4:+59702844	MS.gene60093:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATTTACGAAGAAACTCG+CGG	+	chr8.4:59702780-59702799	MS.gene60093:CDS	35.0%
!!	GTTTATGCTTTGAAGAGACA+AGG	+	chr8.4:59702894-59702913	MS.gene60093:CDS	35.0%
	AGACAAGGAAGGACTTTGTA+TGG	+	chr8.4:59702909-59702928	MS.gene60093:CDS	40.0%
	GGAAGGACTTTGTATGGTTT+CGG	+	chr8.4:59702915-59702934	MS.gene60093:CDS	40.0%
	TTACGTATACTGAACATGCG+AGG	+	chr8.4:59702844-59702863	MS.gene60093:CDS	40.0%
!	CGGTGTGTTGAAGATTTTCT+TGG	+	chr8.4:59702800-59702819	MS.gene60093:CDS	40.0%
!!	ATGCTTTGAAGAGACAAGGA+AGG	+	chr8.4:59702898-59702917	MS.gene60093:CDS	40.0%
	AGGACTTTGTATGGTTTCGG+AGG	+	chr8.4:59702918-59702937	MS.gene60093:CDS	45.0%
!	ATTCGTCGATTAGCGAGAAG+AGG	+	chr8.4:59702735-59702754	MS.gene60093:CDS	45.0%
	CGTATACTGAACATGCGAGG+AGG	+	chr8.4:59702847-59702866	MS.gene60093:CDS	50.0%
	GAGGTGTGAAGAGAATCAGC+GGG	+	chr8.4:59702757-59702776	MS.gene60093:CDS	50.0%
	GGAGGTAAGGGACTAGGAAA+GGG	+	chr8.4:59702651-59702670	MS.gene60093:CDS	50.0%
	GTTCTTCGCGACAACATCCA+AGG	+	chr8.4:59702696-59702715	MS.gene60093:CDS	50.0%
	TGAACATGCGAGGAGGAAGA+CGG	+	chr8.4:59702854-59702873	MS.gene60093:CDS	50.0%
!	GGAGGTGTGAAGAGAATCAG+CGG	+	chr8.4:59702756-59702775	MS.gene60093:CDS	50.0%
!!	TCGCTGGCTTTGTGATTCCT+TGG	-	chr8.4:59702716-59702735	None:intergenic	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	+	chr8.4:59702638-59702657	MS.gene60093:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	+	chr8.4:59702633-59702652	MS.gene60093:CDS	55.0%
	CTCGCTAATCGACGAATCGC+TGG	-	chr8.4:59702732-59702751	None:intergenic	55.0%
	GAGGAAGACGGTGACTGCTA+TGG	+	chr8.4:59702866-59702885	MS.gene60093:CDS	55.0%
	GGAAAGGGAGGTAAGGGACT+AGG	+	chr8.4:59702645-59702664	MS.gene60093:CDS	55.0%
	GGGAGGTAAGGGACTAGGAA+AGG	+	chr8.4:59702650-59702669	MS.gene60093:CDS	55.0%
	GGTAAGGGACTAGGAAAGGG+AGG	+	chr8.4:59702654-59702673	MS.gene60093:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	+	chr8.4:59702639-59702658	MS.gene60093:CDS	55.0%
!	CGTCGATTAGCGAGAAGAGG+AGG	+	chr8.4:59702738-59702757	MS.gene60093:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	59702633	59702944	59702633	ID=MS.gene60093
chr8.4	mRNA	59702633	59702944	59702633	ID=MS.gene60093.t1;Parent=MS.gene60093
chr8.4	exon	59702633	59702944	59702633	ID=MS.gene60093.t1.exon1;Parent=MS.gene60093.t1
chr8.4	CDS	59702633	59702944	59702633	ID=cds.MS.gene60093.t1;Parent=MS.gene60093.t1

Gene Sequence	>MS.gene60093 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTAGGAAAGGGAGGTGCAAAGAGACATCGCAAAGTTCTTCGCGACAACATCCAAGGAATCACAAAGCCAGCGATTCGTCGATTAGCGAGAAGAGGAGGTGTGAAGAGAATCAGCGGGTTGATTTACGAAGAAACTCGCGGTGTGTTGAAGATTTTCTTGGAGAATGTGATAAGAGATGCTGTTACGTATACTGAACATGCGAGGAGGAAGACGGTGACTGCTATGGATGTTGTTTATGCTTTGAAGAGACAAGGAAGGACTTTGTATGGTTTCGGAGGTTGA
Protein sequence	>MS.gene60093.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG