AlfalfaGEDB | MS.gene009027

M. sativa cultivar XinJiangDaYe / MS.gene009027

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009027.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009027.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009027.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene009027	MS.gene035721	PPI
MS.gene009027	MS.gene061870	PPI
MS.gene30920	MS.gene009027	PPI
MS.gene009027	MS.gene072493	PPI
MS.gene009027	MS.gene84536	PPI
MS.gene009027	MS.gene035896	PPI
MS.gene009027	MS.gene88681	PPI
MS.gene009027	MS.gene82546	PPI
MS.gene009027	MS.gene60093	PPI
MS.gene009027	MS.gene08122	PPI
MS.gene009027	MS.gene061869	PPI
MS.gene009027	MS.gene035718	PPI
MS.gene009027	MS.gene022932	PPI
MS.gene012391	MS.gene009027	PPI
MS.gene009027	MS.gene010220	PPI
MS.gene070244	MS.gene009027	PPI
MS.gene009027	MS.gene04972	PPI
MS.gene009027	MS.gene004121	PPI
MS.gene009027	MS.gene62520	PPI
MS.gene009027	MS.gene018578	PPI
MS.gene009027	MS.gene061867	PPI
MS.gene009027	MS.gene04064	PPI
MS.gene31196	MS.gene009027	PPI
MS.gene009027	MS.gene018580	PPI
MS.gene009027	MS.gene004124	PPI
MS.gene009027	MS.gene88680	PPI
MS.gene009027	MS.gene30920	PPI
MS.gene060465	MS.gene009027	PPI
MS.gene009027	MS.gene05619	PPI
MS.gene009027	MS.gene04052	PPI
MS.gene009027	MS.gene061872	PPI
MS.gene009027	MS.gene061866	PPI
MS.gene009027	MS.gene64650	PPI
MS.gene009027	MS.gene79195	PPI
MS.gene009027	MS.gene048705	PPI
MS.gene009027	MS.gene061874	PPI
MS.gene009027	MS.gene04576	PPI
MS.gene71215	MS.gene009027	PPI
MS.gene061870	MS.gene009027	PPI
MS.gene009027	MS.gene64920	PPI
MS.gene035718	MS.gene009027	PPI
MS.gene009027	MS.gene04452	PPI
MS.gene009027	MS.gene070244	PPI
MS.gene009027	MS.gene89487	PPI
MS.gene071136	MS.gene009027	PPI
MS.gene009027	MS.gene038469	PPI
MS.gene009027	MS.gene008276	PPI
MS.gene78578	MS.gene009027	PPI
MS.gene009027	MS.gene73136	PPI
MS.gene009027	MS.gene049821	PPI
MS.gene009027	MS.gene04068	PPI
MS.gene009027	MS.gene022946	PPI
MS.gene009027	MS.gene035719	PPI
MS.gene009027	MS.gene05623	PPI
MS.gene009027	MS.gene89488	PPI
MS.gene028629	MS.gene009027	PPI
MS.gene009027	MS.gene58854	PPI
MS.gene009027	MS.gene66225	PPI
MS.gene009027	MS.gene035720	PPI
MS.gene009027	MS.gene70955	PPI
MS.gene009027	MS.gene61017	PPI
MS.gene009027	MS.gene09871	PPI
MS.gene035787	MS.gene009027	PPI
MS.gene04067	MS.gene009027	PPI
MS.gene009027	MS.gene012390	PPI
MS.gene009027	MS.gene08121	PPI
MS.gene009027	MS.gene064557	PPI
MS.gene009027	MS.gene049705	PPI
MS.gene009027	MS.gene04434	PPI
MS.gene009027	MS.gene070907	PPI
MS.gene009027	MS.gene04392	PPI
MS.gene009027	MS.gene010219	PPI
MS.gene009027	MS.gene04433	PPI
MS.gene009027	MS.gene05608	PPI
MS.gene009027	MS.gene040383	PPI
MS.gene009027	MS.gene68545	PPI
MS.gene061872	MS.gene009027	PPI
MS.gene009027	MS.gene070242	PPI
MS.gene009027	MS.gene016624	PPI
MS.gene88680	MS.gene009027	PPI
MS.gene070241	MS.gene009027	PPI
MS.gene056215	MS.gene009027	PPI
MS.gene009027	MS.gene048746	PPI
MS.gene009027	MS.gene09873	PPI
MS.gene009027	MS.gene028629	PPI
MS.gene061876	MS.gene009027	PPI
MS.gene009027	MS.gene043967	PPI
MS.gene009027	MS.gene31196	PPI
MS.gene009027	MS.gene060465	PPI
MS.gene009027	MS.gene08053	PPI
MS.gene060463	MS.gene009027	PPI
MS.gene009027	MS.gene035897	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009027.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene009027.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene009027.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene009027.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene009027.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene009027.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene009027.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene009027.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene009027.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene009027.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene009027.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene009027.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene009027.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene009027.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene009027.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene009027.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009027.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene009027.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene009027.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene009027.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene009027.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene009027.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene009027.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene009027.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene009027.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene009027.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene009027.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 29 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGAGAACTCTTTACGGTTT+CGG	0.376970	8.2:-36176802	MS.gene009027:CDS
TCGCAGGCTTTGTTATTCCT+TGG	0.407291	8.2:+36177004	None:intergenic
AAAATGTCAGGTCGCGGAAA+AGG	0.432832	8.2:-36177087	MS.gene009027:CDS
GGAGGAAAGGGATTGGGAAA+AGG	0.436575	8.2:-36177066	MS.gene009027:CDS
AGACAGGGGAGAACTCTTTA+CGG	0.442868	8.2:-36176808	MS.gene009027:CDS
TGTTTATGCTTTGAAGAGAC+AGG	0.461410	8.2:-36176824	MS.gene009027:CDS
GAGGAAAACCGTTACGGCTA+TGG	0.462806	8.2:-36176851	MS.gene009027:CDS
GGAAAAGGAGGAAAGGGATT+GGG	0.479340	8.2:-36177072	MS.gene009027:CDS
CGGAAAAGGAGGAAAGGGAT+TGG	0.487643	8.2:-36177073	MS.gene009027:CDS
CGAGCTAAACGACGAATCGC+AGG	0.510292	8.2:+36176988	None:intergenic
TTACTTACACTGAGCATGCT+AGG	0.518049	8.2:-36176873	MS.gene009027:CDS
GGAAAGGGATTGGGAAAAGG+AGG	0.526255	8.2:-36177063	MS.gene009027:CDS
AGGAGCAAAGAGGCATAGAA+AGG	0.536855	8.2:-36177043	MS.gene009027:CDS
TGCTAGGAGGAAAACCGTTA+CGG	0.546899	8.2:-36176857	MS.gene009027:CDS
GGTCGCGGAAAAGGAGGAAA+GGG	0.574670	8.2:-36177078	MS.gene009027:CDS
ATGTCAGGTCGCGGAAAAGG+AGG	0.578066	8.2:-36177084	MS.gene009027:CDS
CGTCGTTTAGCTCGAAGAGG+AGG	0.578607	8.2:-36176979	MS.gene009027:CDS
GTTTATGCTTTGAAGAGACA+GGG	0.590468	8.2:-36176823	MS.gene009027:CDS
GGAGGTGTGAAGAGAATCAG+TGG	0.594114	8.2:-36176961	MS.gene009027:CDS
AGGTCGCGGAAAAGGAGGAA+AGG	0.594714	8.2:-36177079	MS.gene009027:CDS
AACAACATCCATAGCCGTAA+CGG	0.596499	8.2:+36176843	None:intergenic
AGAACTCTTTACGGTTTCGG+TGG	0.599890	8.2:-36176799	MS.gene009027:CDS
GTTCTTCGTGACAACATCCA+AGG	0.625870	8.2:-36177021	MS.gene009027:CDS
ATTCGTCGTTTAGCTCGAAG+AGG	0.657397	8.2:-36176982	MS.gene009027:CDS
TTTATGCTTTGAAGAGACAG+GGG	0.664122	8.2:-36176822	MS.gene009027:CDS
CTTATCTATGAAGAAACTCG+CGG	0.683519	8.2:-36176937	MS.gene009027:CDS
CATCACGAATAACGTTCTCG+AGG	0.697048	8.2:+36176899	None:intergenic
CTTACACTGAGCATGCTAGG+AGG	0.700541	8.2:-36176870	MS.gene009027:CDS
TGGGAAAAGGAGGAGCAAAG+AGG	0.707785	8.2:-36177053	MS.gene009027:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTTATCTATGAAGAAACTCG+CGG	-	chr8.2:36176942-36176961	MS.gene009027:CDS	35.0%
!!	GTTTATGCTTTGAAGAGACA+GGG	-	chr8.2:36177056-36177075	MS.gene009027:CDS	35.0%
!!	TGTTTATGCTTTGAAGAGAC+AGG	-	chr8.2:36177055-36177074	MS.gene009027:CDS	35.0%
!!	TTTATGCTTTGAAGAGACAG+GGG	-	chr8.2:36177057-36177076	MS.gene009027:CDS	35.0%
	AACAACATCCATAGCCGTAA+CGG	+	chr8.2:36177039-36177058	None:intergenic	40.0%
	TTACTTACACTGAGCATGCT+AGG	-	chr8.2:36177006-36177025	MS.gene009027:CDS	40.0%
	AGAACTCTTTACGGTTTCGG+TGG	-	chr8.2:36177080-36177099	MS.gene009027:CDS	45.0%
	AGACAGGGGAGAACTCTTTA+CGG	-	chr8.2:36177071-36177090	MS.gene009027:CDS	45.0%
	CATCACGAATAACGTTCTCG+AGG	+	chr8.2:36176983-36177002	None:intergenic	45.0%
	GGAAAAGGAGGAAAGGGATT+GGG	-	chr8.2:36176807-36176826	MS.gene009027:CDS	45.0%
	GGGAGAACTCTTTACGGTTT+CGG	-	chr8.2:36177077-36177096	MS.gene009027:CDS	45.0%
	GTTCTTCGTGACAACATCCA+AGG	-	chr8.2:36176858-36176877	MS.gene009027:CDS	45.0%
	TGCTAGGAGGAAAACCGTTA+CGG	-	chr8.2:36177022-36177041	MS.gene009027:CDS	45.0%
!	AGGAGCAAAGAGGCATAGAA+AGG	-	chr8.2:36176836-36176855	MS.gene009027:CDS	45.0%
!	ATTCGTCGTTTAGCTCGAAG+AGG	-	chr8.2:36176897-36176916	MS.gene009027:CDS	45.0%
!!	TCGCAGGCTTTGTTATTCCT+TGG	+	chr8.2:36176878-36176897	None:intergenic	45.0%
	CGGAAAAGGAGGAAAGGGAT+TGG	-	chr8.2:36176806-36176825	MS.gene009027:CDS	50.0%
	CTTACACTGAGCATGCTAGG+AGG	-	chr8.2:36177009-36177028	MS.gene009027:CDS	50.0%
	GAGGAAAACCGTTACGGCTA+TGG	-	chr8.2:36177028-36177047	MS.gene009027:CDS	50.0%
	GGAAAGGGATTGGGAAAAGG+AGG	-	chr8.2:36176816-36176835	MS.gene009027:CDS	50.0%
	GGAGGAAAGGGATTGGGAAA+AGG	-	chr8.2:36176813-36176832	MS.gene009027:CDS	50.0%
	TGGGAAAAGGAGGAGCAAAG+AGG	-	chr8.2:36176826-36176845	MS.gene009027:CDS	50.0%
!	GGAGGTGTGAAGAGAATCAG+TGG	-	chr8.2:36176918-36176937	MS.gene009027:CDS	50.0%
	AGGTCGCGGAAAAGGAGGAA+AGG	-	chr8.2:36176800-36176819	MS.gene009027:CDS	55.0%
	ATGTCAGGTCGCGGAAAAGG+AGG	-	chr8.2:36176795-36176814	MS.gene009027:CDS	55.0%
	CGAGCTAAACGACGAATCGC+AGG	+	chr8.2:36176894-36176913	None:intergenic	55.0%
	GGTCGCGGAAAAGGAGGAAA+GGG	-	chr8.2:36176801-36176820	MS.gene009027:CDS	55.0%
!	CGTCGTTTAGCTCGAAGAGG+AGG	-	chr8.2:36176900-36176919	MS.gene009027:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	36176795	36177106	36176795	ID=MS.gene009027
chr8.2	mRNA	36176795	36177106	36176795	ID=MS.gene009027.t1;Parent=MS.gene009027
chr8.2	exon	36176795	36177106	36176795	ID=MS.gene009027.t1.exon1;Parent=MS.gene009027.t1
chr8.2	CDS	36176795	36177106	36176795	ID=cds.MS.gene009027.t1;Parent=MS.gene009027.t1

Gene Sequence	>MS.gene009027 ATGTCAGGTCGCGGAAAAGGAGGAAAGGGATTGGGAAAAGGAGGAGCAAAGAGGCATAGAAAGGTTCTTCGTGACAACATCCAAGGAATAACAAAGCCTGCGATTCGTCGTTTAGCTCGAAGAGGAGGTGTGAAGAGAATCAGTGGTCTTATCTATGAAGAAACTCGCGGTGTTCTCAAGATCTTCCTCGAGAACGTTATTCGTGATGCTGTTACTTACACTGAGCATGCTAGGAGGAAAACCGTTACGGCTATGGATGTTGTTTATGCTTTGAAGAGACAGGGGAGAACTCTTTACGGTTTCGGTGGTTGA
Protein sequence	>MS.gene009027.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG