AlfalfaGEDB | MS.gene061870

M. sativa cultivar XinJiangDaYe / MS.gene061870

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061870.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061870.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061870.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene061870	MS.gene016624	PPI
MS.gene009027	MS.gene061870	PPI
MS.gene061870	MS.gene82546	PPI
MS.gene31196	MS.gene061870	PPI
MS.gene061870	MS.gene048746	PPI
MS.gene060465	MS.gene061870	PPI
MS.gene061870	MS.gene09873	PPI
MS.gene061870	MS.gene028629	PPI
MS.gene028629	MS.gene061870	PPI
MS.gene061870	MS.gene043967	PPI
MS.gene061870	MS.gene060465	PPI
MS.gene061870	MS.gene08053	PPI
MS.gene04067	MS.gene061870	PPI
MS.gene71215	MS.gene061870	PPI
MS.gene061870	MS.gene035897	PPI
MS.gene061870	MS.gene035721	PPI
MS.gene035718	MS.gene061870	PPI
MS.gene071136	MS.gene061870	PPI
MS.gene061870	MS.gene072493	PPI
MS.gene061870	MS.gene84536	PPI
MS.gene061870	MS.gene035896	PPI
MS.gene061870	MS.gene88681	PPI
MS.gene78578	MS.gene061870	PPI
MS.gene061870	MS.gene64650	PPI
MS.gene061870	MS.gene60093	PPI
MS.gene061870	MS.gene08122	PPI
MS.gene061870	MS.gene061869	PPI
MS.gene061870	MS.gene035718	PPI
MS.gene061870	MS.gene022932	PPI
MS.gene061870	MS.gene010220	PPI
MS.gene061870	MS.gene04972	PPI
MS.gene061870	MS.gene004121	PPI
MS.gene061870	MS.gene62520	PPI
MS.gene035787	MS.gene061870	PPI
MS.gene061870	MS.gene018578	PPI
MS.gene061870	MS.gene009027	PPI
MS.gene061870	MS.gene061867	PPI
MS.gene061870	MS.gene04064	PPI
MS.gene061870	MS.gene018580	PPI
MS.gene061870	MS.gene004124	PPI
MS.gene061870	MS.gene88680	PPI
MS.gene061870	MS.gene30920	PPI
MS.gene061870	MS.gene05619	PPI
MS.gene30920	MS.gene061870	PPI
MS.gene061870	MS.gene04052	PPI
MS.gene061870	MS.gene061872	PPI
MS.gene061870	MS.gene061866	PPI
MS.gene061872	MS.gene061870	PPI
MS.gene061870	MS.gene79195	PPI
MS.gene061870	MS.gene048705	PPI
MS.gene88680	MS.gene061870	PPI
MS.gene061870	MS.gene061874	PPI
MS.gene061870	MS.gene04576	PPI
MS.gene070241	MS.gene061870	PPI
MS.gene056215	MS.gene061870	PPI
MS.gene061870	MS.gene64920	PPI
MS.gene061876	MS.gene061870	PPI
MS.gene061870	MS.gene04452	PPI
MS.gene061870	MS.gene070244	PPI
MS.gene061870	MS.gene89487	PPI
MS.gene012391	MS.gene061870	PPI
MS.gene061870	MS.gene038469	PPI
MS.gene060463	MS.gene061870	PPI
MS.gene061870	MS.gene008276	PPI
MS.gene061870	MS.gene73136	PPI
MS.gene061870	MS.gene049821	PPI
MS.gene061870	MS.gene04068	PPI
MS.gene061870	MS.gene022946	PPI
MS.gene061870	MS.gene035719	PPI
MS.gene061870	MS.gene05623	PPI
MS.gene061870	MS.gene89488	PPI
MS.gene061870	MS.gene58854	PPI
MS.gene061870	MS.gene66225	PPI
MS.gene061870	MS.gene035720	PPI
MS.gene061870	MS.gene70955	PPI
MS.gene061870	MS.gene61017	PPI
MS.gene061870	MS.gene09871	PPI
MS.gene061870	MS.gene31196	PPI
MS.gene061870	MS.gene012390	PPI
MS.gene061870	MS.gene08121	PPI
MS.gene061870	MS.gene064557	PPI
MS.gene061870	MS.gene049705	PPI
MS.gene061870	MS.gene04434	PPI
MS.gene061870	MS.gene070907	PPI
MS.gene061870	MS.gene04392	PPI
MS.gene061870	MS.gene010219	PPI
MS.gene070244	MS.gene061870	PPI
MS.gene061870	MS.gene04433	PPI
MS.gene061870	MS.gene05608	PPI
MS.gene061870	MS.gene040383	PPI
MS.gene061870	MS.gene68545	PPI
MS.gene061870	MS.gene070242	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061870.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061870.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061870.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061870.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061870.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061870.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061870.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061870.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061870.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061870.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061870.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061870.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061870.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061870.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061870.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene061870.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061870.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061870.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061870.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061870.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061870.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061870.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061870.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061870.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061870.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061870.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061870.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTGTTCTCAAGATCTTCT+TGG	0.324000	7.4:+10728110	MS.gene061870:CDS
GGAAAGGGATTGGGAAAGGG+AGG	0.412951	7.4:+10727964	MS.gene061870:CDS
TCGCAGGCTTTGTTATGCCT+TGG	0.436279	7.4:-10728023	None:intergenic
AAAATGTCTGGCCGCGGAAA+AGG	0.446222	7.4:+10727940	None:intergenic
TGTTTATGCTTTGAAGAGAC+AGG	0.447929	7.4:+10728203	MS.gene061870:CDS
GGAGGAAAGGGATTGGGAAA+GGG	0.462159	7.4:+10727961	MS.gene061870:CDS
GGAAGGACTCTCTACGGTTT+CGG	0.462635	7.4:+10728225	MS.gene061870:CDS
AGGAGGAAAGGGATTGGGAA+AGG	0.466050	7.4:+10727960	MS.gene061870:CDS
GGAAAAGGAGGAAAGGGATT+GGG	0.479340	7.4:+10727955	MS.gene061870:CDS
CGGAAAAGGAGGAAAGGGAT+TGG	0.482086	7.4:+10727954	MS.gene061870:CDS
CTGCGATTCGTCGTCTTGCA+AGG	0.502831	7.4:+10728040	MS.gene061870:CDS
GGCCGCGGAAAAGGAGGAAA+GGG	0.550993	7.4:+10727949	MS.gene061870:CDS
CTTGCAAGACGACGAATCGC+AGG	0.552192	7.4:-10728039	None:intergenic
AGGAGCAAAGAGGCACAGAA+AGG	0.564264	7.4:+10727984	MS.gene061870:CDS
TTACTTACACTGAGCACGCT+AGG	0.572434	7.4:+10728154	MS.gene061870:CDS
ATGTCTGGCCGCGGAAAAGG+AGG	0.578066	7.4:+10727943	MS.gene061870:CDS
GAGGAAGACTGTTACCGCCA+TGG	0.588179	7.4:+10728176	MS.gene061870:CDS
TGGCCGCGGAAAAGGAGGAA+AGG	0.594714	7.4:+10727948	MS.gene061870:CDS
AGACAGGGAAGGACTCTCTA+CGG	0.604561	7.4:+10728219	MS.gene061870:CDS
AGGACTCTCTACGGTTTCGG+TGG	0.607233	7.4:+10728228	MS.gene061870:CDS
CAAAGCATAAACAACATCCA+TGG	0.612707	7.4:-10728193	None:intergenic
CGTCGTCTTGCAAGGCGTGG+TGG	0.615268	7.4:+10728048	MS.gene061870:CDS
GTTTATGCTTTGAAGAGACA+GGG	0.618996	7.4:+10728204	MS.gene061870:CDS
ATGCTTTGAAGAGACAGGGA+AGG	0.619457	7.4:+10728208	MS.gene061870:CDS
ATTCGTCGTCTTGCAAGGCG+TGG	0.619919	7.4:+10728045	MS.gene061870:CDS
GTTCTTCGCGATAACATCCA+AGG	0.633409	7.4:+10728006	MS.gene061870:CDS
AGCGTGCTCAGTGTAAGTAA+CGG	0.650649	7.4:-10728151	None:intergenic
CTTACACTGAGCACGCTAGG+AGG	0.678435	7.4:+10728157	MS.gene061870:CDS
TGGGAAAGGGAGGAGCAAAG+AGG	0.685544	7.4:+10727974	MS.gene061870:CDS
GGTGGTGTGAAGAGAATCAG+TGG	0.694412	7.4:+10728066	MS.gene061870:CDS
CTCATCTATGAAGAGACTCG+CGG	0.697662	7.4:+10728090	MS.gene061870:CDS
AGCATAAACAACATCCATGG+CGG	0.725411	7.4:-10728190	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CAAAGCATAAACAACATCCA+TGG	-	chr7.4:10728196-10728215	None:intergenic	35.0%
!!	GTTTATGCTTTGAAGAGACA+GGG	+	chr7.4:10728204-10728223	MS.gene061870:CDS	35.0%
!!	TGTTTATGCTTTGAAGAGAC+AGG	+	chr7.4:10728203-10728222	MS.gene061870:CDS	35.0%
	AGCATAAACAACATCCATGG+CGG	-	chr7.4:10728193-10728212	None:intergenic	40.0%
	AGCGTGCTCAGTGTAAGTAA+CGG	-	chr7.4:10728154-10728173	None:intergenic	45.0%
	CGGTGTTCTCAAGATCTTCT+TGG	+	chr7.4:10728110-10728129	MS.gene061870:CDS	45.0%
	CTCATCTATGAAGAGACTCG+CGG	+	chr7.4:10728090-10728109	MS.gene061870:CDS	45.0%
	GGAAAAGGAGGAAAGGGATT+GGG	+	chr7.4:10727955-10727974	MS.gene061870:CDS	45.0%
	GTTCTTCGCGATAACATCCA+AGG	+	chr7.4:10728006-10728025	MS.gene061870:CDS	45.0%
	TTACTTACACTGAGCACGCT+AGG	+	chr7.4:10728154-10728173	MS.gene061870:CDS	45.0%
!!	ATGCTTTGAAGAGACAGGGA+AGG	+	chr7.4:10728208-10728227	MS.gene061870:CDS	45.0%
	AGACAGGGAAGGACTCTCTA+CGG	+	chr7.4:10728219-10728238	MS.gene061870:CDS	50.0%
	AGGAGGAAAGGGATTGGGAA+AGG	+	chr7.4:10727960-10727979	MS.gene061870:CDS	50.0%
	CGGAAAAGGAGGAAAGGGAT+TGG	+	chr7.4:10727954-10727973	MS.gene061870:CDS	50.0%
	GGAAGGACTCTCTACGGTTT+CGG	+	chr7.4:10728225-10728244	MS.gene061870:CDS	50.0%
	GGAGGAAAGGGATTGGGAAA+GGG	+	chr7.4:10727961-10727980	MS.gene061870:CDS	50.0%
	GGTGGTGTGAAGAGAATCAG+TGG	+	chr7.4:10728066-10728085	MS.gene061870:CDS	50.0%
!	AGGAGCAAAGAGGCACAGAA+AGG	+	chr7.4:10727984-10728003	MS.gene061870:CDS	50.0%
!	ATCCCTTTCCTCCTTTTCCG+CGG	-	chr7.4:10727954-10727973	None:intergenic	50.0%
!!	TCGCAGGCTTTGTTATGCCT+TGG	-	chr7.4:10728026-10728045	None:intergenic	50.0%
	AGGACTCTCTACGGTTTCGG+TGG	+	chr7.4:10728228-10728247	MS.gene061870:CDS	55.0%
	ATTCGTCGTCTTGCAAGGCG+TGG	+	chr7.4:10728045-10728064	MS.gene061870:CDS	55.0%
	CTGCGATTCGTCGTCTTGCA+AGG	+	chr7.4:10728040-10728059	MS.gene061870:CDS	55.0%
	CTTACACTGAGCACGCTAGG+AGG	+	chr7.4:10728157-10728176	MS.gene061870:CDS	55.0%
	CTTGCAAGACGACGAATCGC+AGG	-	chr7.4:10728042-10728061	None:intergenic	55.0%
	GAGGAAGACTGTTACCGCCA+TGG	+	chr7.4:10728176-10728195	MS.gene061870:CDS	55.0%
	GGAAAGGGATTGGGAAAGGG+AGG	+	chr7.4:10727964-10727983	MS.gene061870:CDS	55.0%
	TGGGAAAGGGAGGAGCAAAG+AGG	+	chr7.4:10727974-10727993	MS.gene061870:CDS	55.0%
	ATGTCTGGCCGCGGAAAAGG+AGG	+	chr7.4:10727943-10727962	MS.gene061870:CDS	60.0%
	GGCCGCGGAAAAGGAGGAAA+GGG	+	chr7.4:10727949-10727968	MS.gene061870:CDS	60.0%
	TGGCCGCGGAAAAGGAGGAA+AGG	+	chr7.4:10727948-10727967	MS.gene061870:CDS	60.0%
	CGTCGTCTTGCAAGGCGTGG+TGG	+	chr7.4:10728048-10728067	MS.gene061870:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	10727943	10728254	10727943	ID=MS.gene061870
chr7.4	mRNA	10727943	10728254	10727943	ID=MS.gene061870.t1;Parent=MS.gene061870
chr7.4	exon	10727943	10728254	10727943	ID=MS.gene061870.t1.exon1;Parent=MS.gene061870.t1
chr7.4	CDS	10727943	10728254	10727943	ID=cds.MS.gene061870.t1;Parent=MS.gene061870.t1

Gene Sequence	>MS.gene061870 ATGTCTGGCCGCGGAAAAGGAGGAAAGGGATTGGGAAAGGGAGGAGCAAAGAGGCACAGAAAGGTTCTTCGCGATAACATCCAAGGCATAACAAAGCCTGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTGAAGAGAATCAGTGGTCTCATCTATGAAGAGACTCGCGGTGTTCTCAAGATCTTCTTGGAGAACGTGATTCGTGATGCCGTTACTTACACTGAGCACGCTAGGAGGAAGACTGTTACCGCCATGGATGTTGTTTATGCTTTGAAGAGACAGGGAAGGACTCTCTACGGTTTCGGTGGTTAA
Protein sequence	>MS.gene061870.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG