AlfalfaGEDB | MS.gene012390

M. sativa cultivar XinJiangDaYe / MS.gene012390

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012390.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012390.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012390.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene79195	MS.gene012390	PPI
MS.gene88680	MS.gene012390	PPI
MS.gene056215	MS.gene012390	PPI
MS.gene064557	MS.gene012390	PPI
MS.gene049821	MS.gene012390	PPI
MS.gene62520	MS.gene012390	PPI
MS.gene012390	MS.gene010219	PPI
MS.gene038469	MS.gene012390	PPI
MS.gene012390	MS.gene68545	PPI
MS.gene61017	MS.gene012390	PPI
MS.gene04452	MS.gene012390	PPI
MS.gene012390	MS.gene016624	PPI
MS.gene035787	MS.gene012390	PPI
MS.gene09871	MS.gene012390	PPI
MS.gene04576	MS.gene012390	PPI
MS.gene89487	MS.gene012390	PPI
MS.gene04392	MS.gene012390	PPI
MS.gene30920	MS.gene012390	PPI
MS.gene08053	MS.gene012390	PPI
MS.gene061872	MS.gene012390	PPI
MS.gene04068	MS.gene012390	PPI
MS.gene012390	MS.gene049705	PPI
MS.gene070241	MS.gene012390	PPI
MS.gene04434	MS.gene012390	PPI
MS.gene022946	MS.gene012390	PPI
MS.gene012390	MS.gene05608	PPI
MS.gene04064	MS.gene012390	PPI
MS.gene061876	MS.gene012390	PPI
MS.gene05608	MS.gene012390	PPI
MS.gene022932	MS.gene012390	PPI
MS.gene012390	MS.gene84536	PPI
MS.gene012391	MS.gene012390	PPI
MS.gene88681	MS.gene012390	PPI
MS.gene070244	MS.gene012390	PPI
MS.gene035897	MS.gene012390	PPI
MS.gene64920	MS.gene012390	PPI
MS.gene060463	MS.gene012390	PPI
MS.gene012390	MS.gene60093	PPI
MS.gene070907	MS.gene012390	PPI
MS.gene012390	MS.gene09873	PPI
MS.gene31196	MS.gene012390	PPI
MS.gene010219	MS.gene012390	PPI
MS.gene060465	MS.gene012390	PPI
MS.gene012390	MS.gene04972	PPI
MS.gene012390	MS.gene004121	PPI
MS.gene012390	MS.gene62520	PPI
MS.gene012390	MS.gene018578	PPI
MS.gene012390	MS.gene018580	PPI
MS.gene012390	MS.gene004124	PPI
MS.gene08121	MS.gene012390	PPI
MS.gene012390	MS.gene04052	PPI
MS.gene012390	MS.gene061866	PPI
MS.gene035718	MS.gene012390	PPI
MS.gene035896	MS.gene012390	PPI
MS.gene73136	MS.gene012390	PPI
MS.gene012390	MS.gene64650	PPI
MS.gene008276	MS.gene012390	PPI
MS.gene012390	MS.gene048705	PPI
MS.gene012390	MS.gene061874	PPI
MS.gene012390	MS.gene04576	PPI
MS.gene009027	MS.gene012390	PPI
MS.gene012390	MS.gene061869	PPI
MS.gene09873	MS.gene012390	PPI
MS.gene78578	MS.gene012390	PPI
MS.gene035721	MS.gene012390	PPI
MS.gene04052	MS.gene012390	PPI
MS.gene08122	MS.gene012390	PPI
MS.gene048705	MS.gene012390	PPI
MS.gene05623	MS.gene012390	PPI
MS.gene043967	MS.gene012390	PPI
MS.gene84536	MS.gene012390	PPI
MS.gene028629	MS.gene012390	PPI
MS.gene82546	MS.gene012390	PPI
MS.gene012390	MS.gene061867	PPI
MS.gene04433	MS.gene012390	PPI
MS.gene012390	MS.gene008276	PPI
MS.gene012390	MS.gene05619	PPI
MS.gene012390	MS.gene73136	PPI
MS.gene04067	MS.gene012390	PPI
MS.gene71215	MS.gene012390	PPI
MS.gene061866	MS.gene012390	PPI
MS.gene061870	MS.gene012390	PPI
MS.gene012390	MS.gene89488	PPI
MS.gene035719	MS.gene012390	PPI
MS.gene58854	MS.gene012390	PPI
MS.gene012390	MS.gene035720	PPI
MS.gene012390	MS.gene61017	PPI
MS.gene66225	MS.gene012390	PPI
MS.gene70955	MS.gene012390	PPI
MS.gene071136	MS.gene012390	PPI
MS.gene040383	MS.gene012390	PPI
MS.gene070242	MS.gene012390	PPI
MS.gene072493	MS.gene012390	PPI
MS.gene035720	MS.gene012390	PPI
MS.gene048746	MS.gene012390	PPI
MS.gene010220	MS.gene012390	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012390.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012390.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012390.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012390.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012390.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012390.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012390.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012390.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012390.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012390.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012390.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012390.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012390.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012390.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene012390.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene012390.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012390.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012390.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012390.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012390.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012390.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012390.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012390.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012390.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012390.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012390.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene012390.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 30 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGCAGGGAAGGACTCTTTA+TGG	0.247348	8.3:-3059264	MS.gene012390:CDS
GGAAGGACTCTTTATGGATT+TGG	0.297351	8.3:-3059258	MS.gene012390:CDS
GGCAAAGGAGGAAAGGGTTT+AGG	0.384869	8.3:-3059528	MS.gene012390:CDS
GGAGGAAAGGGTTTAGGAAA+GGG	0.429069	8.3:-3059522	MS.gene012390:CDS
TCTTTATGGATTTGGCGGTT+AGG	0.439485	8.3:-3059250	None:intergenic
AGAAATTTCACAGCAATGTC+AGG	0.466987	8.3:-3059555	None:intergenic
TCGCAGGCTTTGTAATTCCC+TGG	0.486609	8.3:+3059460	None:intergenic
CGTTTACGCTCTCAAGAGGC+AGG	0.488613	8.3:-3059280	MS.gene012390:CDS
AGGACTCTTTATGGATTTGG+CGG	0.509079	8.3:-3059255	MS.gene012390:CDS
AGGAAGAGGCAAAGGAGGAA+AGG	0.518412	8.3:-3059535	MS.gene012390:CDS
GGTTCTTCGTGATAACATCC+AGG	0.522451	8.3:-3059478	MS.gene012390:CDS
GGAAGAGGCAAAGGAGGAAA+GGG	0.527098	8.3:-3059534	MS.gene012390:CDS
AGGAGGAAAGGGTTTAGGAA+AGG	0.528235	8.3:-3059523	MS.gene012390:CDS
GGAAAGGGTTTAGGAAAGGG+AGG	0.536831	8.3:-3059519	MS.gene012390:CDS
CCGTAAAACTGTTACTGCGA+TGG	0.549023	8.3:-3059307	MS.gene012390:CDS
ACGGCGAGCATGCTCTGTGT+AGG	0.562056	8.3:+3059326	None:intergenic
GCAATGTCAGGAAGAGGCAA+AGG	0.564054	8.3:-3059543	MS.gene012390:CDS
TGTTATCACGAAGAACCTTA+CGG	0.569497	8.3:+3059484	None:intergenic
TTCACAGCAATGTCAGGAAG+AGG	0.572997	8.3:-3059549	None:intergenic
AGGAGCAAAACGACACCGTA+AGG	0.575994	8.3:-3059499	MS.gene012390:CDS
ATGTCAGGAAGAGGCAAAGG+AGG	0.581057	8.3:-3059540	MS.gene012390:CDS
ACGCTCTCAAGAGGCAGGGA+AGG	0.583359	8.3:-3059275	MS.gene012390:CDS
CTTGCAAGACGACGAATCGC+AGG	0.588436	8.3:+3059444	None:intergenic
GTTCTTCGTGATAACATCCA+GGG	0.626731	8.3:-3059477	MS.gene012390:CDS
CGTCGTCTTGCAAGACGTGG+TGG	0.633046	8.3:-3059435	MS.gene012390:CDS
TTAATCTATGAAGAAACTCG+TGG	0.634992	8.3:-3059393	MS.gene012390:CDS
ATGTCGTTTACGCTCTCAAG+AGG	0.636714	8.3:-3059284	MS.gene012390:CDS
ATTCGTCGTCTTGCAAGACG+TGG	0.646636	8.3:-3059438	MS.gene012390:CDS
GTTTACGCTCTCAAGAGGCA+GGG	0.663401	8.3:-3059279	MS.gene012390:CDS
GGTGGTGTGAAGCGTATCAG+TGG	0.669421	8.3:-3059417	MS.gene012390:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTAATCTATGAAGAAACTCG+TGG	-	chr8.3:3059398-3059417	MS.gene012390:CDS	30.0%
	TGTTATCACGAAGAACCTTA+CGG	+	chr8.3:3059310-3059329	None:intergenic	35.0%
	AGGACTCTTTATGGATTTGG+CGG	-	chr8.3:3059536-3059555	MS.gene012390:CDS	40.0%
	GGAAGGACTCTTTATGGATT+TGG	-	chr8.3:3059533-3059552	MS.gene012390:CDS	40.0%
	GTTCTTCGTGATAACATCCA+GGG	-	chr8.3:3059314-3059333	MS.gene012390:CDS	40.0%
!	CCATCGCAGTAACAGTTTTA+CGG	+	chr8.3:3059487-3059506	None:intergenic	40.0%
	AGGAGGAAAGGGTTTAGGAA+AGG	-	chr8.3:3059268-3059287	MS.gene012390:CDS	45.0%
	CCGTAAAACTGTTACTGCGA+TGG	-	chr8.3:3059484-3059503	MS.gene012390:CDS	45.0%
	GGAGGAAAGGGTTTAGGAAA+GGG	-	chr8.3:3059269-3059288	MS.gene012390:CDS	45.0%
	GGTTCTTCGTGATAACATCC+AGG	-	chr8.3:3059313-3059332	MS.gene012390:CDS	45.0%
!	ATGTCGTTTACGCTCTCAAG+AGG	-	chr8.3:3059507-3059526	MS.gene012390:CDS	45.0%
	AGGAAGAGGCAAAGGAGGAA+AGG	-	chr8.3:3059256-3059275	MS.gene012390:CDS	50.0%
	AGGAGCAAAACGACACCGTA+AGG	-	chr8.3:3059292-3059311	MS.gene012390:CDS	50.0%
	AGGCAGGGAAGGACTCTTTA+TGG	-	chr8.3:3059527-3059546	MS.gene012390:CDS	50.0%
	ATGTCAGGAAGAGGCAAAGG+AGG	-	chr8.3:3059251-3059270	MS.gene012390:CDS	50.0%
	ATTCGTCGTCTTGCAAGACG+TGG	-	chr8.3:3059353-3059372	MS.gene012390:CDS	50.0%
	GGAAAGGGTTTAGGAAAGGG+AGG	-	chr8.3:3059272-3059291	MS.gene012390:CDS	50.0%
	GGAAGAGGCAAAGGAGGAAA+GGG	-	chr8.3:3059257-3059276	MS.gene012390:CDS	50.0%
	GGCAAAGGAGGAAAGGGTTT+AGG	-	chr8.3:3059263-3059282	MS.gene012390:CDS	50.0%
	GTTTACGCTCTCAAGAGGCA+GGG	-	chr8.3:3059512-3059531	MS.gene012390:CDS	50.0%
!	TCGCAGGCTTTGTAATTCCC+TGG	+	chr8.3:3059334-3059353	None:intergenic	50.0%
	CGTTTACGCTCTCAAGAGGC+AGG	-	chr8.3:3059511-3059530	MS.gene012390:CDS	55.0%
	CTTGCAAGACGACGAATCGC+AGG	+	chr8.3:3059350-3059369	None:intergenic	55.0%
	GGTGGTGTGAAGCGTATCAG+TGG	-	chr8.3:3059374-3059393	MS.gene012390:CDS	55.0%
	ACGCTCTCAAGAGGCAGGGA+AGG	-	chr8.3:3059516-3059535	MS.gene012390:CDS	60.0%
	ACGGCGAGCATGCTCTGTGT+AGG	+	chr8.3:3059468-3059487	None:intergenic	60.0%
	CGTCGTCTTGCAAGACGTGG+TGG	-	chr8.3:3059356-3059375	MS.gene012390:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	3059251	3059562	3059251	ID=MS.gene012390
chr8.3	mRNA	3059251	3059562	3059251	ID=MS.gene012390.t1;Parent=MS.gene012390
chr8.3	exon	3059251	3059562	3059251	ID=MS.gene012390.t1.exon1;Parent=MS.gene012390.t1
chr8.3	CDS	3059251	3059562	3059251	ID=cds.MS.gene012390.t1;Parent=MS.gene012390.t1

Gene Sequence	>MS.gene012390 ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTTTAGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTTCGTGATAACATCCAGGGAATTACAAAGCCTGCGATTCGTCGTCTTGCAAGACGTGGTGGTGTGAAGCGTATCAGTGGCTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAATGTGATTCGTGATGCTGTCACCTACACAGAGCATGCTCGCCGTAAAACTGTTACTGCGATGGATGTCGTTTACGCTCTCAAGAGGCAGGGAAGGACTCTTTATGGATTTGGCGGTTAG
Protein sequence	>MS.gene012390.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG