AlfalfaGEDB | MS.gene035897

M. sativa cultivar XinJiangDaYe / MS.gene035897

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035897.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035897.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035897.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene78578	MS.gene035897	PPI
MS.gene060465	MS.gene035897	PPI
MS.gene035897	MS.gene04052	PPI
MS.gene035897	MS.gene84536	PPI
MS.gene05623	MS.gene035897	PPI
MS.gene043967	MS.gene035897	PPI
MS.gene035897	MS.gene061866	PPI
MS.gene035897	MS.gene64650	PPI
MS.gene028629	MS.gene035897	PPI
MS.gene035897	MS.gene79195	PPI
MS.gene035897	MS.gene048705	PPI
MS.gene035897	MS.gene061874	PPI
MS.gene035897	MS.gene061869	PPI
MS.gene04067	MS.gene035897	PPI
MS.gene71215	MS.gene035897	PPI
MS.gene061870	MS.gene035897	PPI
MS.gene035897	MS.gene004121	PPI
MS.gene035897	MS.gene061867	PPI
MS.gene66225	MS.gene035897	PPI
MS.gene071136	MS.gene035897	PPI
MS.gene035897	MS.gene04064	PPI
MS.gene070242	MS.gene035897	PPI
MS.gene035897	MS.gene008276	PPI
MS.gene035897	MS.gene05619	PPI
MS.gene035897	MS.gene73136	PPI
MS.gene79195	MS.gene035897	PPI
MS.gene035721	MS.gene035897	PPI
MS.gene035897	MS.gene049821	PPI
MS.gene035897	MS.gene04068	PPI
MS.gene035897	MS.gene05623	PPI
MS.gene056215	MS.gene035897	PPI
MS.gene035897	MS.gene89488	PPI
MS.gene064557	MS.gene035897	PPI
MS.gene038469	MS.gene035897	PPI
MS.gene035897	MS.gene61017	PPI
MS.gene035897	MS.gene04576	PPI
MS.gene04433	MS.gene035897	PPI
MS.gene04452	MS.gene035897	PPI
MS.gene035897	MS.gene09871	PPI
MS.gene035897	MS.gene012390	PPI
MS.gene035787	MS.gene035897	PPI
MS.gene035897	MS.gene04434	PPI
MS.gene89487	MS.gene035897	PPI
MS.gene035897	MS.gene04392	PPI
MS.gene035719	MS.gene035897	PPI
MS.gene035897	MS.gene010219	PPI
MS.gene58854	MS.gene035897	PPI
MS.gene035897	MS.gene68545	PPI
MS.gene70955	MS.gene035897	PPI
MS.gene040383	MS.gene035897	PPI
MS.gene04392	MS.gene035897	PPI
MS.gene035897	MS.gene016624	PPI
MS.gene072493	MS.gene035897	PPI
MS.gene30920	MS.gene035897	PPI
MS.gene048746	MS.gene035897	PPI
MS.gene010220	MS.gene035897	PPI
MS.gene08053	MS.gene035897	PPI
MS.gene061872	MS.gene035897	PPI
MS.gene035897	MS.gene048746	PPI
MS.gene88680	MS.gene035897	PPI
MS.gene070241	MS.gene035897	PPI
MS.gene022946	MS.gene035897	PPI
MS.gene035897	MS.gene035720	PPI
MS.gene061876	MS.gene035897	PPI
MS.gene012391	MS.gene035897	PPI
MS.gene070244	MS.gene035897	PPI
MS.gene09871	MS.gene035897	PPI
MS.gene035897	MS.gene049705	PPI
MS.gene64920	MS.gene035897	PPI
MS.gene060463	MS.gene035897	PPI
MS.gene035897	MS.gene070907	PPI
MS.gene035897	MS.gene05608	PPI
MS.gene31196	MS.gene035897	PPI
MS.gene035897	MS.gene82546	PPI
MS.gene04068	MS.gene035897	PPI
MS.gene035897	MS.gene60093	PPI
MS.gene04434	MS.gene035897	PPI
MS.gene035897	MS.gene08122	PPI
MS.gene035897	MS.gene09873	PPI
MS.gene08121	MS.gene035897	PPI
MS.gene022932	MS.gene035897	PPI
MS.gene035897	MS.gene04972	PPI
MS.gene88681	MS.gene035897	PPI
MS.gene035897	MS.gene62520	PPI
MS.gene035718	MS.gene035897	PPI
MS.gene035896	MS.gene035897	PPI
MS.gene035897	MS.gene018578	PPI
MS.gene035897	MS.gene018580	PPI
MS.gene035897	MS.gene004124	PPI
MS.gene009027	MS.gene035897	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035897.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035897.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035897.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035897.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035897.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035897.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035897.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035897.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035897.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035897.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035897.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035897.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035897.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035897.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035897.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene035897.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035897.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035897.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035897.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035897.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035897.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035897.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035897.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035897.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035897.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035897.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035897.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 29 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAAGGACCCTTTATGGTTT+TGG	0.243182	5.2:-53272881	MS.gene035897:CDS
AGACAAGGAAGGACCCTTTA+TGG	0.291085	5.2:-53272887	MS.gene035897:CDS
GGAAAAGGAGGAAAGGGTTT+AGG	0.350988	5.2:-53273151	MS.gene035897:CDS
AGTTCTTCGAGATAACATTC+AGG	0.362931	5.2:-53273101	MS.gene035897:CDS
GAGACGACGAATCGCGGGTT+TGG	0.448204	5.2:+53273072	None:intergenic
GGAGGAAAGGGTTTAGGAAA+GGG	0.452389	5.2:-53273145	MS.gene035897:CDS
GGAAGAGGAAAAGGAGGAAA+GGG	0.454700	5.2:-53273157	MS.gene035897:CDS
TCGCAAAACGGTGACTGCTA+TGG	0.458269	5.2:-53272930	MS.gene035897:CDS
ATCATGTCAGGAAGAGGAAA+AGG	0.468345	5.2:-53273166	MS.gene035897:CDS
TGTTATCTCGAAGAACTTTG+CGG	0.480041	5.2:+53273107	None:intergenic
AGGAAGAGGAAAAGGAGGAA+AGG	0.488546	5.2:-53273158	MS.gene035897:CDS
GTTCTTCGAGATAACATTCA+GGG	0.506869	5.2:-53273100	MS.gene035897:CDS
ATCTTGCAATCAATCATGTC+AGG	0.507815	5.2:-53273178	None:intergenic
CAATCAATCATGTCAGGAAG+AGG	0.521728	5.2:-53273172	None:intergenic
GCGACGAGCGTGTTCCGTGT+AGG	0.529617	5.2:+53272949	None:intergenic
CCGCGATTCGTCGTCTCGCA+AGG	0.536114	5.2:-53273066	MS.gene035897:CDS
AGGAGGAAAGGGTTTAGGAA+AGG	0.547494	5.2:-53273146	MS.gene035897:CDS
CTTGCGAGACGACGAATCGC+GGG	0.552203	5.2:+53273067	None:intergenic
GGAACACGCTCGTCGCAAAA+CGG	0.562995	5.2:-53272942	MS.gene035897:CDS
GGAAAGGGTTTAGGAAAGGG+TGG	0.573172	5.2:-53273142	MS.gene035897:CDS
CCTTGCGAGACGACGAATCG+CGG	0.599403	5.2:+53273066	None:intergenic
ATTCGTCGTCTCGCAAGGCG+TGG	0.610829	5.2:-53273061	MS.gene035897:CDS
ATGTCAGGAAGAGGAAAAGG+AGG	0.630961	5.2:-53273163	MS.gene035897:CDS
CGTCGTCTCGCAAGGCGTGG+TGG	0.632134	5.2:-53273058	MS.gene035897:CDS
GGTGGTGTTAAGCGTATCAG+TGG	0.637620	5.2:-53273040	MS.gene035897:CDS
GTTTATGCACTCAAGAGACA+AGG	0.649593	5.2:-53272902	MS.gene035897:CDS
TCGTGATGCTGTAACCTACA+CGG	0.656428	5.2:-53272963	MS.gene035897:CDS
CTCATCTATGAAGAAACTCG+TGG	0.682677	5.2:-53273016	MS.gene035897:CDS
ATGCACTCAAGAGACAAGGA+AGG	0.704360	5.2:-53272898	MS.gene035897:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGTTCTTCGAGATAACATTC+AGG	-	chr5.2:53272936-53272955	MS.gene035897:CDS	35.0%
	GTTCTTCGAGATAACATTCA+GGG	-	chr5.2:53272937-53272956	MS.gene035897:CDS	35.0%
	TGTTATCTCGAAGAACTTTG+CGG	+	chr5.2:53272933-53272952	None:intergenic	35.0%
	CTAACCCCCAAAACCATAAA+GGG	+	chr5.2:53273166-53273185	None:intergenic	40.0%
	CTCATCTATGAAGAAACTCG+TGG	-	chr5.2:53273021-53273040	MS.gene035897:CDS	40.0%
	GTTTATGCACTCAAGAGACA+AGG	-	chr5.2:53273135-53273154	MS.gene035897:CDS	40.0%
!	GAAGGACCCTTTATGGTTTT+GGG	-	chr5.2:53273157-53273176	MS.gene035897:CDS	40.0%
!!	AAGGACCCTTTATGGTTTTG+GGG	-	chr5.2:53273158-53273177	MS.gene035897:CDS	40.0%
	AGACAAGGAAGGACCCTTTA+TGG	-	chr5.2:53273150-53273169	MS.gene035897:CDS	45.0%
	AGGAAGAGGAAAAGGAGGAA+AGG	-	chr5.2:53272879-53272898	MS.gene035897:CDS	45.0%
	AGGAGGAAAGGGTTTAGGAA+AGG	-	chr5.2:53272891-53272910	MS.gene035897:CDS	45.0%
	ATGCACTCAAGAGACAAGGA+AGG	-	chr5.2:53273139-53273158	MS.gene035897:CDS	45.0%
	ATGTCAGGAAGAGGAAAAGG+AGG	-	chr5.2:53272874-53272893	MS.gene035897:CDS	45.0%
	GGAAAAGGAGGAAAGGGTTT+AGG	-	chr5.2:53272886-53272905	MS.gene035897:CDS	45.0%
	GGAAGAGGAAAAGGAGGAAA+GGG	-	chr5.2:53272880-53272899	MS.gene035897:CDS	45.0%
	GGAGGAAAGGGTTTAGGAAA+GGG	-	chr5.2:53272892-53272911	MS.gene035897:CDS	45.0%
	TCGTGATGCTGTAACCTACA+CGG	-	chr5.2:53273074-53273093	MS.gene035897:CDS	45.0%
!	GGAAGGACCCTTTATGGTTT+TGG	-	chr5.2:53273156-53273175	MS.gene035897:CDS	45.0%
!!!	AGGACCCTTTATGGTTTTGG+GGG	-	chr5.2:53273159-53273178	MS.gene035897:CDS	45.0%
	GGAAAGGGTTTAGGAAAGGG+TGG	-	chr5.2:53272895-53272914	MS.gene035897:CDS	50.0%
	TCGCAAAACGGTGACTGCTA+TGG	-	chr5.2:53273107-53273126	MS.gene035897:CDS	50.0%
!	GGTGGTGTTAAGCGTATCAG+TGG	-	chr5.2:53272997-53273016	MS.gene035897:CDS	50.0%
	GGAACACGCTCGTCGCAAAA+CGG	-	chr5.2:53273095-53273114	MS.gene035897:CDS	55.0%
	ATTCGTCGTCTCGCAAGGCG+TGG	-	chr5.2:53272976-53272995	MS.gene035897:CDS	60.0%
	CCTTGCGAGACGACGAATCG+CGG	+	chr5.2:53272974-53272993	None:intergenic	60.0%
	CTTGCGAGACGACGAATCGC+GGG	+	chr5.2:53272973-53272992	None:intergenic	60.0%
	GAGACGACGAATCGCGGGTT+TGG	+	chr5.2:53272968-53272987	None:intergenic	60.0%
	CCGCGATTCGTCGTCTCGCA+AGG	-	chr5.2:53272971-53272990	MS.gene035897:CDS	65.0%
	GCGACGAGCGTGTTCCGTGT+AGG	+	chr5.2:53273091-53273110	None:intergenic	65.0%
	CGTCGTCTCGCAAGGCGTGG+TGG	-	chr5.2:53272979-53272998	MS.gene035897:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	53272874	53273185	53272874	ID=MS.gene035897
chr5.2	mRNA	53272874	53273185	53272874	ID=MS.gene035897.t1;Parent=MS.gene035897
chr5.2	exon	53272874	53273185	53272874	ID=MS.gene035897.t1.exon1;Parent=MS.gene035897.t1
chr5.2	CDS	53272874	53273185	53272874	ID=cds.MS.gene035897.t1;Parent=MS.gene035897.t1

Gene Sequence	>MS.gene035897 ATGTCAGGAAGAGGAAAAGGAGGAAAGGGTTTAGGAAAGGGTGGTGCAAAGCGACACCGCAAAGTTCTTCGAGATAACATTCAGGGAATTACCAAACCCGCGATTCGTCGTCTCGCAAGGCGTGGTGGTGTTAAGCGTATCAGTGGTCTCATCTATGAAGAAACTCGTGGTGTTCTCAAGATATTTCTCGAGAATGTGATTCGTGATGCTGTAACCTACACGGAACACGCTCGTCGCAAAACGGTGACTGCTATGGATGTTGTTTATGCACTCAAGAGACAAGGAAGGACCCTTTATGGTTTTGGGGGTTAG
Protein sequence	>MS.gene035897.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG