AlfalfaGEDB | MS.gene89487

M. sativa cultivar XinJiangDaYe / MS.gene89487

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89487.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89487.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89487.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene89487	0.820319	7.30E-53	-1.69E-46
MS.gene049501	MS.gene89487	0.812584	3.94E-51	-1.69E-46
MS.gene049821	MS.gene89487	0.848973	4.36E-60	-1.69E-46
MS.gene050020	MS.gene89487	0.832146	1.12E-55	-1.69E-46
MS.gene050807	MS.gene89487	0.839828	1.25E-57	-1.69E-46
MS.gene050901	MS.gene89487	0.848789	4.90E-60	-1.69E-46
MS.gene051198	MS.gene89487	0.815462	9.13E-52	-1.69E-46
MS.gene051247	MS.gene89487	0.833226	6.02E-56	-1.69E-46
MS.gene051279	MS.gene89487	0.804994	1.65E-49	-1.69E-46
MS.gene051719	MS.gene89487	0.835095	2.05E-56	-1.69E-46
MS.gene051720	MS.gene89487	0.835897	1.28E-56	-1.69E-46
MS.gene052133	MS.gene89487	0.80377	2.98E-49	-1.69E-46
MS.gene052516	MS.gene89487	0.818661	1.74E-52	-1.69E-46
MS.gene052665	MS.gene89487	0.811917	5.51E-51	-1.69E-46
MS.gene053226	MS.gene89487	0.805554	1.26E-49	-1.69E-46
MS.gene053581	MS.gene89487	0.821592	3.72E-53	-1.69E-46
MS.gene05364	MS.gene89487	0.815924	7.20E-52	-1.69E-46
MS.gene054651	MS.gene89487	0.806508	7.95E-50	-1.69E-46
MS.gene055465	MS.gene89487	0.834754	2.50E-56	-1.69E-46
MS.gene055466	MS.gene89487	0.845054	5.16E-59	-1.69E-46
MS.gene055468	MS.gene89487	0.824443	8.05E-54	-1.69E-46
MS.gene055469	MS.gene89487	0.808982	2.37E-50	-1.69E-46
MS.gene055818	MS.gene89487	0.807553	4.78E-50	-1.69E-46
MS.gene055926	MS.gene89487	0.808893	2.48E-50	-1.69E-46
MS.gene056215	MS.gene89487	0.814475	1.51E-51	-1.69E-46
MS.gene056308	MS.gene89487	0.836909	7.10E-57	-1.69E-46
MS.gene056366	MS.gene89487	0.816461	5.47E-52	-1.69E-46
MS.gene056507	MS.gene89487	0.803588	3.24E-49	-1.69E-46
MS.gene056553	MS.gene89487	0.811036	8.56E-51	-1.69E-46
MS.gene056595	MS.gene89487	0.811933	5.46E-51	-1.69E-46
MS.gene057444	MS.gene89487	0.811032	8.57E-51	-1.69E-46
MS.gene058625	MS.gene89487	0.811707	6.12E-51	-1.69E-46
MS.gene060587	MS.gene89487	0.840455	8.61E-58	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene89487	MS.gene061867	PPI
MS.gene071136	MS.gene89487	PPI
MS.gene89487	MS.gene008276	PPI
MS.gene89487	MS.gene05619	PPI
MS.gene89487	MS.gene73136	PPI
MS.gene89487	MS.gene049821	PPI
MS.gene89487	MS.gene04068	PPI
MS.gene88680	MS.gene89487	PPI
MS.gene89487	MS.gene022946	PPI
MS.gene89487	MS.gene035719	PPI
MS.gene89487	MS.gene05623	PPI
MS.gene89487	MS.gene89488	PPI
MS.gene056215	MS.gene89487	PPI
MS.gene89487	MS.gene58854	PPI
MS.gene89487	MS.gene66225	PPI
MS.gene89487	MS.gene035720	PPI
MS.gene89487	MS.gene70955	PPI
MS.gene89487	MS.gene61017	PPI
MS.gene89487	MS.gene09871	PPI
MS.gene89487	MS.gene012390	PPI
MS.gene89487	MS.gene08121	PPI
MS.gene89487	MS.gene064557	PPI
MS.gene89487	MS.gene049705	PPI
MS.gene89487	MS.gene04434	PPI
MS.gene89487	MS.gene070907	PPI
MS.gene060463	MS.gene89487	PPI
MS.gene89487	MS.gene04392	PPI
MS.gene89487	MS.gene010219	PPI
MS.gene89487	MS.gene04433	PPI
MS.gene89487	MS.gene05608	PPI
MS.gene89487	MS.gene040383	PPI
MS.gene89487	MS.gene68545	PPI
MS.gene89487	MS.gene070242	PPI
MS.gene89487	MS.gene016624	PPI
MS.gene30920	MS.gene89487	PPI
MS.gene061872	MS.gene89487	PPI
MS.gene89487	MS.gene048746	PPI
MS.gene070241	MS.gene89487	PPI
MS.gene89487	MS.gene09873	PPI
MS.gene89487	MS.gene043967	PPI
MS.gene061876	MS.gene89487	PPI
MS.gene012391	MS.gene89487	PPI
MS.gene88681	MS.gene89487	PPI
MS.gene89487	MS.gene08053	PPI
MS.gene070244	MS.gene89487	PPI
MS.gene89487	MS.gene035897	PPI
MS.gene89487	MS.gene035721	PPI
MS.gene009027	MS.gene89487	PPI
MS.gene31196	MS.gene89487	PPI
MS.gene89487	MS.gene072493	PPI
MS.gene060465	MS.gene89487	PPI
MS.gene89487	MS.gene84536	PPI
MS.gene89487	MS.gene035896	PPI
MS.gene89487	MS.gene88681	PPI
MS.gene89487	MS.gene82546	PPI
MS.gene89487	MS.gene60093	PPI
MS.gene89487	MS.gene08122	PPI
MS.gene89487	MS.gene022932	PPI
MS.gene061870	MS.gene89487	PPI
MS.gene89487	MS.gene010220	PPI
MS.gene89487	MS.gene04972	PPI
MS.gene89487	MS.gene004121	PPI
MS.gene89487	MS.gene62520	PPI
MS.gene035718	MS.gene89487	PPI
MS.gene89487	MS.gene018578	PPI
MS.gene89487	MS.gene04064	PPI
MS.gene89487	MS.gene018580	PPI
MS.gene89487	MS.gene004124	PPI
MS.gene78578	MS.gene89487	PPI
MS.gene89487	MS.gene04052	PPI
MS.gene89487	MS.gene061866	PPI
MS.gene89487	MS.gene64650	PPI
MS.gene028629	MS.gene89487	PPI
MS.gene89487	MS.gene79195	PPI
MS.gene89487	MS.gene048705	PPI
MS.gene89487	MS.gene061874	PPI
MS.gene89487	MS.gene04576	PPI
MS.gene04433	MS.gene89487	PPI
MS.gene89487	MS.gene061869	PPI
MS.gene89487	MS.gene64920	PPI
MS.gene035787	MS.gene89487	PPI
MS.gene04067	MS.gene89487	PPI
MS.gene71215	MS.gene89487	PPI
MS.gene89487	MS.gene04452	PPI
MS.gene58854	MS.gene89487	PPI
MS.gene89487	MS.gene038469	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89487.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89487.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89487.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89487.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89487.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89487.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89487.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89487.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89487.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89487.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89487.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89487.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89487.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89487.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89487.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene89487.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89487.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89487.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89487.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89487.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89487.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89487.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89487.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89487.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89487.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89487.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89487.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 31 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGCAAGGAAGGACCCTTTA+TGG	0.289623	5.4:-49496317	MS.gene89487:CDS
GGAAGGACCCTTTATGGTTT+CGG	0.347466	5.4:-49496311	MS.gene89487:CDS
CCTAACCACCGAAACCATAA+AGG	0.360537	5.4:+49496303	None:intergenic
CCTTTATGGTTTCGGTGGTT+AGG	0.360991	5.4:-49496303	None:intergenic
GGCAAAGGAGGAAAGGGTTT+AGG	0.384869	5.4:-49496581	MS.gene89487:CDS
GTTCTTAGAGATAACATTCA+AGG	0.421661	5.4:-49496530	MS.gene89487:CDS
TCGCAAAACGGTGACTGCTA+TGG	0.458269	5.4:-49496360	MS.gene89487:CDS
ATCTTGCAATCAACAATGTC+AGG	0.473641	5.4:-49496608	None:intergenic
CTAACCACCGAAACCATAAA+GGG	0.505014	5.4:+49496304	None:intergenic
GGAGGAAAGGGTTTAGGAAA+GGG	0.517349	5.4:-49496575	MS.gene89487:CDS
AGGAAGAGGCAAAGGAGGAA+AGG	0.518412	5.4:-49496588	MS.gene89487:CDS
CCGCGATTCGTCGTCTTGCA+AGG	0.525617	5.4:-49496496	MS.gene89487:CDS
GGAAGAGGCAAAGGAGGAAA+GGG	0.527098	5.4:-49496587	MS.gene89487:CDS
CTTGCAAGACGACGAATCGC+GGG	0.554223	5.4:+49496497	None:intergenic
TGAACATGCTCGTCGCAAAA+CGG	0.556730	5.4:-49496372	MS.gene89487:CDS
CCTTGCAAGACGACGAATCG+CGG	0.571292	5.4:+49496496	None:intergenic
CAATCAACAATGTCAGGAAG+AGG	0.577531	5.4:-49496602	None:intergenic
ATGTGGTTTACGCGCTCAAG+AGG	0.592981	5.4:-49496337	MS.gene89487:CDS
ACGCGCTCAAGAGGCAAGGA+AGG	0.594884	5.4:-49496328	MS.gene89487:CDS
AGGACCCTTTATGGTTTCGG+TGG	0.597859	5.4:-49496308	MS.gene89487:CDS
AACGGTGACTGCTATGGATG+TGG	0.598005	5.4:-49496354	MS.gene89487:CDS
ACAATGTCAGGAAGAGGCAA+AGG	0.598135	5.4:-49496596	MS.gene89487:CDS
ATGTCAGGAAGAGGCAAAGG+AGG	0.604597	5.4:-49496593	MS.gene89487:CDS
CGTCGTCTTGCAAGGCGTGG+TGG	0.615268	5.4:-49496488	MS.gene89487:CDS
AGGAGGAAAGGGTTTAGGAA+AGG	0.616515	5.4:-49496576	MS.gene89487:CDS
ATTCGTCGTCTTGCAAGGCG+TGG	0.619919	5.4:-49496491	MS.gene89487:CDS
GTTTACGCGCTCAAGAGGCA+AGG	0.623143	5.4:-49496332	MS.gene89487:CDS
TATCTCTAAGAACTTTGCGA+TGG	0.632919	5.4:+49496540	None:intergenic
CTTATCTATGAAGAAACTCG+TGG	0.644041	5.4:-49496446	MS.gene89487:CDS
GGTGGTGTGAAGCGTATCAG+TGG	0.661880	5.4:-49496470	MS.gene89487:CDS
GGAAAGGGTTTAGGAAAGGG+CGG	0.700891	5.4:-49496572	MS.gene89487:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GTTCTTAGAGATAACATTCA+AGG	-	chr5.4:49496367-49496386	MS.gene89487:CDS	30.0%
	CTTATCTATGAAGAAACTCG+TGG	-	chr5.4:49496451-49496470	MS.gene89487:CDS	35.0%
!	TATCTCTAAGAACTTTGCGA+TGG	+	chr5.4:49496360-49496379	None:intergenic	35.0%
	CTAACCACCGAAACCATAAA+GGG	+	chr5.4:49496596-49496615	None:intergenic	40.0%
	AGGAGGAAAGGGTTTAGGAA+AGG	-	chr5.4:49496321-49496340	MS.gene89487:CDS	45.0%
	GGAAGGACCCTTTATGGTTT+CGG	-	chr5.4:49496586-49496605	MS.gene89487:CDS	45.0%
	GGAGGAAAGGGTTTAGGAAA+GGG	-	chr5.4:49496322-49496341	MS.gene89487:CDS	45.0%
	TGAACATGCTCGTCGCAAAA+CGG	-	chr5.4:49496525-49496544	MS.gene89487:CDS	45.0%
	AACGGTGACTGCTATGGATG+TGG	-	chr5.4:49496543-49496562	MS.gene89487:CDS	50.0%
	AGGAAGAGGCAAAGGAGGAA+AGG	-	chr5.4:49496309-49496328	MS.gene89487:CDS	50.0%
	AGGACCCTTTATGGTTTCGG+TGG	-	chr5.4:49496589-49496608	MS.gene89487:CDS	50.0%
	AGGCAAGGAAGGACCCTTTA+TGG	-	chr5.4:49496580-49496599	MS.gene89487:CDS	50.0%
	ATGTCAGGAAGAGGCAAAGG+AGG	-	chr5.4:49496304-49496323	MS.gene89487:CDS	50.0%
	ATGTGGTTTACGCGCTCAAG+AGG	-	chr5.4:49496560-49496579	MS.gene89487:CDS	50.0%
	GGAAAGGGTTTAGGAAAGGG+CGG	-	chr5.4:49496325-49496344	MS.gene89487:CDS	50.0%
	GGAAGAGGCAAAGGAGGAAA+GGG	-	chr5.4:49496310-49496329	MS.gene89487:CDS	50.0%
	GGCAAAGGAGGAAAGGGTTT+AGG	-	chr5.4:49496316-49496335	MS.gene89487:CDS	50.0%
	TCGCAAAACGGTGACTGCTA+TGG	-	chr5.4:49496537-49496556	MS.gene89487:CDS	50.0%
	ATTCGTCGTCTTGCAAGGCG+TGG	-	chr5.4:49496406-49496425	MS.gene89487:CDS	55.0%
	CCTTGCAAGACGACGAATCG+CGG	+	chr5.4:49496404-49496423	None:intergenic	55.0%
	CTTGCAAGACGACGAATCGC+GGG	+	chr5.4:49496403-49496422	None:intergenic	55.0%
	GGTGGTGTGAAGCGTATCAG+TGG	-	chr5.4:49496427-49496446	MS.gene89487:CDS	55.0%
	GTTTACGCGCTCAAGAGGCA+AGG	-	chr5.4:49496565-49496584	MS.gene89487:CDS	55.0%
	ACGCGCTCAAGAGGCAAGGA+AGG	-	chr5.4:49496569-49496588	MS.gene89487:CDS	60.0%
	CCGCGATTCGTCGTCTTGCA+AGG	-	chr5.4:49496401-49496420	MS.gene89487:CDS	60.0%
	CGTCGTCTTGCAAGGCGTGG+TGG	-	chr5.4:49496409-49496428	MS.gene89487:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	49496304	49496615	49496304	ID=MS.gene89487
chr5.4	mRNA	49496304	49496615	49496304	ID=MS.gene89487.t1;Parent=MS.gene89487
chr5.4	exon	49496304	49496615	49496304	ID=MS.gene89487.t1.exon1;Parent=MS.gene89487.t1
chr5.4	CDS	49496304	49496615	49496304	ID=cds.MS.gene89487.t1;Parent=MS.gene89487.t1

Gene Sequence	>MS.gene89487 ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTTTAGGAAAGGGCGGTGCAAAGCGCCATCGCAAAGTTCTTAGAGATAACATTCAAGGAATAACGAAACCCGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTGAAGCGTATCAGTGGTCTTATCTATGAAGAAACTCGTGGTGTTCTTAAGATATTTCTTGAGAATGTTATTCGCGATGCTGTAACTTACACTGAACATGCTCGTCGCAAAACGGTGACTGCTATGGATGTGGTTTACGCGCTCAAGAGGCAAGGAAGGACCCTTTATGGTTTCGGTGGTTAG
Protein sequence	>MS.gene89487.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG