AlfalfaGEDB | MS.gene66225

M. sativa cultivar XinJiangDaYe / MS.gene66225

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66225.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66225.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66225.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene064557	MS.gene66225	PPI
MS.gene66225	MS.gene04392	PPI
MS.gene038469	MS.gene66225	PPI
MS.gene66225	MS.gene010219	PPI
MS.gene66225	MS.gene68545	PPI
MS.gene04452	MS.gene66225	PPI
MS.gene66225	MS.gene016624	PPI
MS.gene035787	MS.gene66225	PPI
MS.gene89487	MS.gene66225	PPI
MS.gene66225	MS.gene048746	PPI
MS.gene66225	MS.gene022946	PPI
MS.gene66225	MS.gene035720	PPI
MS.gene30920	MS.gene66225	PPI
MS.gene66225	MS.gene043967	PPI
MS.gene010220	MS.gene66225	PPI
MS.gene08053	MS.gene66225	PPI
MS.gene061872	MS.gene66225	PPI
MS.gene66225	MS.gene035897	PPI
MS.gene66225	MS.gene049705	PPI
MS.gene070241	MS.gene66225	PPI
MS.gene66225	MS.gene070907	PPI
MS.gene66225	MS.gene05608	PPI
MS.gene061876	MS.gene66225	PPI
MS.gene022932	MS.gene66225	PPI
MS.gene66225	MS.gene070242	PPI
MS.gene012391	MS.gene66225	PPI
MS.gene88681	MS.gene66225	PPI
MS.gene070244	MS.gene66225	PPI
MS.gene66225	MS.gene82546	PPI
MS.gene64920	MS.gene66225	PPI
MS.gene060463	MS.gene66225	PPI
MS.gene66225	MS.gene60093	PPI
MS.gene66225	MS.gene08122	PPI
MS.gene66225	MS.gene09873	PPI
MS.gene31196	MS.gene66225	PPI
MS.gene66225	MS.gene022932	PPI
MS.gene060465	MS.gene66225	PPI
MS.gene66225	MS.gene010220	PPI
MS.gene66225	MS.gene04972	PPI
MS.gene66225	MS.gene62520	PPI
MS.gene66225	MS.gene018578	PPI
MS.gene66225	MS.gene035721	PPI
MS.gene66225	MS.gene018580	PPI
MS.gene66225	MS.gene004124	PPI
MS.gene08121	MS.gene66225	PPI
MS.gene66225	MS.gene072493	PPI
MS.gene66225	MS.gene04052	PPI
MS.gene66225	MS.gene84536	PPI
MS.gene66225	MS.gene035896	PPI
MS.gene66225	MS.gene061866	PPI
MS.gene035718	MS.gene66225	PPI
MS.gene035896	MS.gene66225	PPI
MS.gene66225	MS.gene64650	PPI
MS.gene66225	MS.gene79195	PPI
MS.gene66225	MS.gene048705	PPI
MS.gene66225	MS.gene061874	PPI
MS.gene009027	MS.gene66225	PPI
MS.gene66225	MS.gene061869	PPI
MS.gene78578	MS.gene66225	PPI
MS.gene035721	MS.gene66225	PPI
MS.gene043967	MS.gene66225	PPI
MS.gene66225	MS.gene004121	PPI
MS.gene028629	MS.gene66225	PPI
MS.gene66225	MS.gene73136	PPI
MS.gene04433	MS.gene66225	PPI
MS.gene66225	MS.gene061867	PPI
MS.gene66225	MS.gene04064	PPI
MS.gene66225	MS.gene008276	PPI
MS.gene04067	MS.gene66225	PPI
MS.gene71215	MS.gene66225	PPI
MS.gene66225	MS.gene05619	PPI
MS.gene061870	MS.gene66225	PPI
MS.gene66225	MS.gene049821	PPI
MS.gene66225	MS.gene04068	PPI
MS.gene66225	MS.gene05623	PPI
MS.gene035719	MS.gene66225	PPI
MS.gene66225	MS.gene89488	PPI
MS.gene58854	MS.gene66225	PPI
MS.gene70955	MS.gene66225	PPI
MS.gene071136	MS.gene66225	PPI
MS.gene040383	MS.gene66225	PPI
MS.gene66225	MS.gene61017	PPI
MS.gene070242	MS.gene66225	PPI
MS.gene66225	MS.gene04576	PPI
MS.gene66225	MS.gene09871	PPI
MS.gene66225	MS.gene012390	PPI
MS.gene88680	MS.gene66225	PPI
MS.gene66225	MS.gene04434	PPI
MS.gene056215	MS.gene66225	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66225.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene66225.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene66225.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene66225.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene66225.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene66225.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene66225.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene66225.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene66225.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene66225.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene66225.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene66225.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene66225.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene66225.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene66225.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene66225.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66225.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene66225.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene66225.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene66225.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene66225.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene66225.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene66225.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene66225.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene66225.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene66225.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene66225.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 40 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGCCAATCGACGAATTGC+AGG	0.295956	3.3:-47033275	None:intergenic
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	3.3:+47033346	MS.gene66225:CDS
GGAAGAACCCTCTATGGTTT+CGG	0.433408	3.3:+47033461	MS.gene66225:CDS
AGGCAGGGAAGAACCCTCTA+TGG	0.440512	3.3:+47033455	MS.gene66225:CDS
GGGAGGTAAGGGACTTGGAA+AGG	0.449159	3.3:+47033196	MS.gene66225:CDS
GGAGGTAAGGGACTTGGAAA+GGG	0.467307	3.3:+47033197	MS.gene66225:CDS
GGAAAGGGAGGTAAGGGACT+TGG	0.470633	3.3:+47033191	MS.gene66225:CDS
TTCAACCTCCGAAACCATAG+AGG	0.481091	3.3:-47033469	None:intergenic
ATTTCCTCTTGGAAAATGTC+AGG	0.482279	3.3:+47033164	None:intergenic
CAATCGACGAATTGCAGGCT+TGG	0.486859	3.3:-47033270	None:intergenic
AGGCCGTGGAAAGGGAGGTA+AGG	0.492819	3.3:+47033184	MS.gene66225:CDS
GGTTCTCCGTGACAACATCC+AGG	0.506274	3.3:+47033241	MS.gene66225:CDS
CAAGCCTGCAATTCGTCGAT+TGG	0.509017	3.3:+47033271	MS.gene66225:CDS
GAAGATCTTCAAGACACCTC+TGG	0.510902	3.3:-47033342	None:intergenic
TGTCTATGCACTCAAAAGGC+AGG	0.525428	3.3:+47033439	MS.gene66225:CDS
AGGTGCAAAGAGGCATAGAA+AGG	0.530485	3.3:+47033220	MS.gene66225:CDS
AAAATGTCAGGCCGTGGAAA+GGG	0.531763	3.3:+47033176	None:intergenic
ATGTTGTCTATGCACTCAAA+AGG	0.538317	3.3:+47033435	MS.gene66225:CDS
TGACATACACTGAGCACGCT+AGG	0.543435	3.3:+47033390	MS.gene66225:CDS
TCTTGGAAAATGTCAGGCCG+TGG	0.545322	3.3:+47033170	None:intergenic
GAAAATGTCAGGCCGTGGAA+AGG	0.550606	3.3:+47033175	None:intergenic
GGCCGTGGAAAGGGAGGTAA+GGG	0.552535	3.3:+47033185	MS.gene66225:CDS
GTCCCTTACCTCCCTTTCCA+CGG	0.555891	3.3:-47033187	None:intergenic
GGTAAGGGACTTGGAAAGGG+AGG	0.560743	3.3:+47033200	MS.gene66225:CDS
GGTGGTGTGAAGAGAATCAG+TGG	0.582471	3.3:+47033302	MS.gene66225:CDS
GAGGAAGACTGTGACCGCCA+TGG	0.585556	3.3:+47033412	MS.gene66225:CDS
CGTCGATTGGCAAGAAGAGG+TGG	0.589209	3.3:+47033284	MS.gene66225:CDS
GTCTATGCACTCAAAAGGCA+GGG	0.590557	3.3:+47033440	MS.gene66225:CDS
GAGTGCATAGACAACATCCA+TGG	0.612836	3.3:-47033429	None:intergenic
TTGCAGGCTTGGTGATACCC+TGG	0.613296	3.3:-47033259	None:intergenic
ATTCGTCGATTGGCAAGAAG+AGG	0.624691	3.3:+47033281	MS.gene66225:CDS
AGAACCCTCTATGGTTTCGG+AGG	0.628880	3.3:+47033464	MS.gene66225:CDS
ATGTCAGGCCGTGGAAAGGG+AGG	0.634999	3.3:+47033179	MS.gene66225:CDS
TGATACCCTGGATGTTGTCA+CGG	0.654870	3.3:-47033247	None:intergenic
TCAACCTCCGAAACCATAGA+GGG	0.663751	3.3:-47033468	None:intergenic
GTTCTCCGTGACAACATCCA+GGG	0.666413	3.3:+47033242	MS.gene66225:CDS
CATACACTGAGCACGCTAGG+AGG	0.675420	3.3:+47033393	MS.gene66225:CDS
TTGGAAAGGGAGGTGCAAAG+AGG	0.683014	3.3:+47033210	MS.gene66225:CDS
TTGATATATGAAGAAACCAG+AGG	0.683025	3.3:+47033326	MS.gene66225:CDS
TGCATAGACAACATCCATGG+CGG	0.733443	3.3:-47033426	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATATATGAAGAAACCAG+AGG	+	chr3.3:47033326-47033345	MS.gene66225:CDS	30.0%
	ATGTTGTCTATGCACTCAAA+AGG	+	chr3.3:47033435-47033454	MS.gene66225:CDS	35.0%
	AGGTGTCTTGAAGATCTTCT+TGG	+	chr3.3:47033346-47033365	MS.gene66225:CDS	40.0%
	GAAGATCTTCAAGACACCTC+TGG	-	chr3.3:47033345-47033364	None:intergenic	45.0%
	GAGTGCATAGACAACATCCA+TGG	-	chr3.3:47033432-47033451	None:intergenic	45.0%
	GGAAGAACCCTCTATGGTTT+CGG	+	chr3.3:47033461-47033480	MS.gene66225:CDS	45.0%
	GTCTATGCACTCAAAAGGCA+GGG	+	chr3.3:47033440-47033459	MS.gene66225:CDS	45.0%
	TCAACCTCCGAAACCATAGA+GGG	-	chr3.3:47033471-47033490	None:intergenic	45.0%
	TGATACCCTGGATGTTGTCA+CGG	-	chr3.3:47033250-47033269	None:intergenic	45.0%
	TGCATAGACAACATCCATGG+CGG	-	chr3.3:47033429-47033448	None:intergenic	45.0%
	TGTCTATGCACTCAAAAGGC+AGG	+	chr3.3:47033439-47033458	MS.gene66225:CDS	45.0%
!	AGGTGCAAAGAGGCATAGAA+AGG	+	chr3.3:47033220-47033239	MS.gene66225:CDS	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	+	chr3.3:47033281-47033300	MS.gene66225:CDS	45.0%
	AGAACCCTCTATGGTTTCGG+AGG	+	chr3.3:47033464-47033483	MS.gene66225:CDS	50.0%
	CAAGCCTGCAATTCGTCGAT+TGG	+	chr3.3:47033271-47033290	MS.gene66225:CDS	50.0%
	CAATCGACGAATTGCAGGCT+TGG	-	chr3.3:47033273-47033292	None:intergenic	50.0%
	CTTGCCAATCGACGAATTGC+AGG	-	chr3.3:47033278-47033297	None:intergenic	50.0%
	GGAGGTAAGGGACTTGGAAA+GGG	+	chr3.3:47033197-47033216	MS.gene66225:CDS	50.0%
	GGTGGTGTGAAGAGAATCAG+TGG	+	chr3.3:47033302-47033321	MS.gene66225:CDS	50.0%
	GTTCTCCGTGACAACATCCA+GGG	+	chr3.3:47033242-47033261	MS.gene66225:CDS	50.0%
	TGACATACACTGAGCACGCT+AGG	+	chr3.3:47033390-47033409	MS.gene66225:CDS	50.0%
!	TTGGAAAGGGAGGTGCAAAG+AGG	+	chr3.3:47033210-47033229	MS.gene66225:CDS	50.0%
	AGGCAGGGAAGAACCCTCTA+TGG	+	chr3.3:47033455-47033474	MS.gene66225:CDS	55.0%
	CATACACTGAGCACGCTAGG+AGG	+	chr3.3:47033393-47033412	MS.gene66225:CDS	55.0%
	GGAAAGGGAGGTAAGGGACT+TGG	+	chr3.3:47033191-47033210	MS.gene66225:CDS	55.0%
	GGGAGGTAAGGGACTTGGAA+AGG	+	chr3.3:47033196-47033215	MS.gene66225:CDS	55.0%
	GGTAAGGGACTTGGAAAGGG+AGG	+	chr3.3:47033200-47033219	MS.gene66225:CDS	55.0%
	GGTTCTCCGTGACAACATCC+AGG	+	chr3.3:47033241-47033260	MS.gene66225:CDS	55.0%
	GTCCCTTACCTCCCTTTCCA+CGG	-	chr3.3:47033190-47033209	None:intergenic	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	+	chr3.3:47033284-47033303	MS.gene66225:CDS	55.0%
!!	TTGCAGGCTTGGTGATACCC+TGG	-	chr3.3:47033262-47033281	None:intergenic	55.0%
	AGGCCGTGGAAAGGGAGGTA+AGG	+	chr3.3:47033184-47033203	MS.gene66225:CDS	60.0%
	ATGTCAGGCCGTGGAAAGGG+AGG	+	chr3.3:47033179-47033198	MS.gene66225:CDS	60.0%
	GAGGAAGACTGTGACCGCCA+TGG	+	chr3.3:47033412-47033431	MS.gene66225:CDS	60.0%
	GGCCGTGGAAAGGGAGGTAA+GGG	+	chr3.3:47033185-47033204	MS.gene66225:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	47033179	47033490	47033179	ID=MS.gene66225
chr3.3	mRNA	47033179	47033490	47033179	ID=MS.gene66225.t1;Parent=MS.gene66225
chr3.3	exon	47033179	47033490	47033179	ID=MS.gene66225.t1.exon1;Parent=MS.gene66225.t1
chr3.3	CDS	47033179	47033490	47033179	ID=cds.MS.gene66225.t1;Parent=MS.gene66225.t1

Gene Sequence	>MS.gene66225 ATGTCAGGCCGTGGAAAGGGAGGTAAGGGACTTGGAAAGGGAGGTGCAAAGAGGCATAGAAAGGTTCTCCGTGACAACATCCAGGGTATCACCAAGCCTGCAATTCGTCGATTGGCAAGAAGAGGTGGTGTGAAGAGAATCAGTGGTTTGATATATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAATGTCATTCGTGATGCTGTGACATACACTGAGCACGCTAGGAGGAAGACTGTGACCGCCATGGATGTTGTCTATGCACTCAAAAGGCAGGGAAGAACCCTCTATGGTTTCGGAGGTTGA
Protein sequence	>MS.gene66225.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG