AlfalfaGEDB | MS.gene035718

M. sativa cultivar XinJiangDaYe / MS.gene035718

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035718.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035718.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035718.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene035718	MS.gene05608	PPI
MS.gene035718	MS.gene68545	PPI
MS.gene035718	MS.gene070242	PPI
MS.gene035718	MS.gene88681	PPI
MS.gene035718	MS.gene82546	PPI
MS.gene035718	MS.gene048746	PPI
MS.gene035718	MS.gene60093	PPI
MS.gene035718	MS.gene08122	PPI
MS.gene035718	MS.gene09873	PPI
MS.gene035718	MS.gene028629	PPI
MS.gene035718	MS.gene022932	PPI
MS.gene035718	MS.gene043967	PPI
MS.gene035718	MS.gene010220	PPI
MS.gene009027	MS.gene035718	PPI
MS.gene035718	MS.gene62520	PPI
MS.gene035718	MS.gene060465	PPI
MS.gene035718	MS.gene08053	PPI
MS.gene035718	MS.gene018578	PPI
MS.gene78578	MS.gene035718	PPI
MS.gene035718	MS.gene035721	PPI
MS.gene035718	MS.gene061870	PPI
MS.gene035718	MS.gene018580	PPI
MS.gene035718	MS.gene004124	PPI
MS.gene035718	MS.gene88680	PPI
MS.gene028629	MS.gene035718	PPI
MS.gene035718	MS.gene072493	PPI
MS.gene035718	MS.gene04052	PPI
MS.gene035718	MS.gene84536	PPI
MS.gene035718	MS.gene035896	PPI
MS.gene035718	MS.gene061866	PPI
MS.gene04067	MS.gene035718	PPI
MS.gene71215	MS.gene035718	PPI
MS.gene035718	MS.gene64650	PPI
MS.gene061870	MS.gene035718	PPI
MS.gene035718	MS.gene048705	PPI
MS.gene035718	MS.gene061874	PPI
MS.gene035718	MS.gene061869	PPI
MS.gene071136	MS.gene035718	PPI
MS.gene035718	MS.gene04972	PPI
MS.gene035718	MS.gene070244	PPI
MS.gene035718	MS.gene004121	PPI
MS.gene035718	MS.gene009027	PPI
MS.gene035718	MS.gene061867	PPI
MS.gene035718	MS.gene04064	PPI
MS.gene035718	MS.gene008276	PPI
MS.gene056215	MS.gene035718	PPI
MS.gene035718	MS.gene30920	PPI
MS.gene035718	MS.gene05619	PPI
MS.gene035718	MS.gene04068	PPI
MS.gene035718	MS.gene035719	PPI
MS.gene035718	MS.gene061872	PPI
MS.gene035718	MS.gene89488	PPI
MS.gene035787	MS.gene035718	PPI
MS.gene035718	MS.gene66225	PPI
MS.gene035718	MS.gene70955	PPI
MS.gene035718	MS.gene79195	PPI
MS.gene035718	MS.gene61017	PPI
MS.gene035718	MS.gene04576	PPI
MS.gene035718	MS.gene09871	PPI
MS.gene035718	MS.gene012390	PPI
MS.gene035718	MS.gene08121	PPI
MS.gene035718	MS.gene64920	PPI
MS.gene035718	MS.gene064557	PPI
MS.gene035718	MS.gene04434	PPI
MS.gene035718	MS.gene04452	PPI
MS.gene035718	MS.gene04392	PPI
MS.gene035718	MS.gene89487	PPI
MS.gene30920	MS.gene035718	PPI
MS.gene061872	MS.gene035718	PPI
MS.gene035718	MS.gene010219	PPI
MS.gene035718	MS.gene04433	PPI
MS.gene035718	MS.gene040383	PPI
MS.gene035718	MS.gene038469	PPI
MS.gene88680	MS.gene035718	PPI
MS.gene070241	MS.gene035718	PPI
MS.gene035718	MS.gene016624	PPI
MS.gene035718	MS.gene73136	PPI
MS.gene035718	MS.gene049821	PPI
MS.gene061876	MS.gene035718	PPI
MS.gene035718	MS.gene022946	PPI
MS.gene035718	MS.gene05623	PPI
MS.gene012391	MS.gene035718	PPI
MS.gene070244	MS.gene035718	PPI
MS.gene035718	MS.gene58854	PPI
MS.gene035718	MS.gene035720	PPI
MS.gene060463	MS.gene035718	PPI
MS.gene035718	MS.gene31196	PPI
MS.gene31196	MS.gene035718	PPI
MS.gene035718	MS.gene035897	PPI
MS.gene035718	MS.gene049705	PPI
MS.gene035718	MS.gene070907	PPI
MS.gene060465	MS.gene035718	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035718.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035718.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035718.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035718.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035718.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035718.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035718.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035718.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035718.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035718.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035718.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035718.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035718.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035718.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035718.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene035718.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035718.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035718.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035718.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035718.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035718.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035718.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035718.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035718.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035718.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035718.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035718.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 30 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTCTCCGCGACAACATTC+AGG	0.329692	8.4:-6156829	MS.gene035718:CDS
GGCAAAGGAGGAAAGGGTTT+AGG	0.384869	8.4:-6156879	MS.gene035718:CDS
GGAGGAAAGGGTTTAGGAAA+GGG	0.429069	8.4:-6156873	MS.gene035718:CDS
GGAAGGACTCTTTACGGATT+CGG	0.436444	8.4:-6156609	MS.gene035718:CDS
AGGCAGGGAAGGACTCTTTA+CGG	0.443617	8.4:-6156615	MS.gene035718:CDS
TCAACTTTCGCAGCTATGTC+AGG	0.463992	8.4:-6156906	None:intergenic
TGTTTACGCTCTCAAGAGGC+AGG	0.493747	8.4:-6156631	MS.gene035718:CDS
TGTTGTCGCGGAGAACCTTA+CGG	0.502023	8.4:+6156835	None:intergenic
AGGAAGAGGCAAAGGAGGAA+AGG	0.518412	8.4:-6156886	MS.gene035718:CDS
GGAAGAGGCAAAGGAGGAAA+GGG	0.527098	8.4:-6156885	MS.gene035718:CDS
AGGAGGAAAGGGTTTAGGAA+AGG	0.528235	8.4:-6156874	MS.gene035718:CDS
GGAAAGGGTTTAGGAAAGGG+AGG	0.536831	8.4:-6156870	MS.gene035718:CDS
CCATCGCAGTAACAGTCTTA+CGG	0.545551	8.4:+6156658	None:intergenic
GTTCTCCGCGACAACATTCA+GGG	0.554706	8.4:-6156828	MS.gene035718:CDS
CCGTAAGACTGTTACTGCGA+TGG	0.561095	8.4:-6156658	MS.gene035718:CDS
TTCGCAGCTATGTCAGGAAG+AGG	0.562303	8.4:-6156900	None:intergenic
GCTATGTCAGGAAGAGGCAA+AGG	0.571124	8.4:-6156894	MS.gene035718:CDS
AGGAGCAAAACGACACCGTA+AGG	0.575994	8.4:-6156850	MS.gene035718:CDS
ACGCTCTCAAGAGGCAGGGA+AGG	0.583359	8.4:-6156626	MS.gene035718:CDS
CTTGCAAGACGACGAATCGC+AGG	0.588436	8.4:+6156795	None:intergenic
ACGGCGAGCATGCTCTGTGT+AGG	0.590446	8.4:+6156677	None:intergenic
ATGTCAGGAAGAGGCAAAGG+AGG	0.592343	8.4:-6156891	MS.gene035718:CDS
AGGACTCTTTACGGATTCGG+TGG	0.600535	8.4:-6156606	MS.gene035718:CDS
ATGTTGTTTACGCTCTCAAG+AGG	0.616421	8.4:-6156635	MS.gene035718:CDS
GTTTACGCTCTCAAGAGGCA+GGG	0.624823	8.4:-6156630	MS.gene035718:CDS
CGTCGTCTTGCAAGACGTGG+TGG	0.633046	8.4:-6156786	MS.gene035718:CDS
TTAATCTATGAAGAAACTCG+TGG	0.634992	8.4:-6156744	MS.gene035718:CDS
TAATTCCCTGAATGTTGTCG+CGG	0.640736	8.4:+6156823	None:intergenic
ATTCGTCGTCTTGCAAGACG+TGG	0.646636	8.4:-6156789	MS.gene035718:CDS
GGTGGTGTGAAACGTATCAG+TGG	0.722771	8.4:-6156768	MS.gene035718:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTAATCTATGAAGAAACTCG+TGG	-	chr8.4:6156749-6156768	MS.gene035718:CDS	30.0%
	TAATTCCCTGAATGTTGTCG+CGG	+	chr8.4:6156673-6156692	None:intergenic	40.0%
!	ATGTTGTTTACGCTCTCAAG+AGG	-	chr8.4:6156858-6156877	MS.gene035718:CDS	40.0%
	AGGAGGAAAGGGTTTAGGAA+AGG	-	chr8.4:6156619-6156638	MS.gene035718:CDS	45.0%
	CCATCGCAGTAACAGTCTTA+CGG	+	chr8.4:6156838-6156857	None:intergenic	45.0%
	GGAAGGACTCTTTACGGATT+CGG	-	chr8.4:6156884-6156903	MS.gene035718:CDS	45.0%
	GGAGGAAAGGGTTTAGGAAA+GGG	-	chr8.4:6156620-6156639	MS.gene035718:CDS	45.0%
	AGGAAGAGGCAAAGGAGGAA+AGG	-	chr8.4:6156607-6156626	MS.gene035718:CDS	50.0%
	AGGACTCTTTACGGATTCGG+TGG	-	chr8.4:6156887-6156906	MS.gene035718:CDS	50.0%
	AGGAGCAAAACGACACCGTA+AGG	-	chr8.4:6156643-6156662	MS.gene035718:CDS	50.0%
	AGGCAGGGAAGGACTCTTTA+CGG	-	chr8.4:6156878-6156897	MS.gene035718:CDS	50.0%
	ATGTCAGGAAGAGGCAAAGG+AGG	-	chr8.4:6156602-6156621	MS.gene035718:CDS	50.0%
	ATTCGTCGTCTTGCAAGACG+TGG	-	chr8.4:6156704-6156723	MS.gene035718:CDS	50.0%
	CCGTAAGACTGTTACTGCGA+TGG	-	chr8.4:6156835-6156854	MS.gene035718:CDS	50.0%
	GGAAAGGGTTTAGGAAAGGG+AGG	-	chr8.4:6156623-6156642	MS.gene035718:CDS	50.0%
	GGAAGAGGCAAAGGAGGAAA+GGG	-	chr8.4:6156608-6156627	MS.gene035718:CDS	50.0%
	GGCAAAGGAGGAAAGGGTTT+AGG	-	chr8.4:6156614-6156633	MS.gene035718:CDS	50.0%
	GGTGGTGTGAAACGTATCAG+TGG	-	chr8.4:6156725-6156744	MS.gene035718:CDS	50.0%
	GTTCTCCGCGACAACATTCA+GGG	-	chr8.4:6156665-6156684	MS.gene035718:CDS	50.0%
	GTTTACGCTCTCAAGAGGCA+GGG	-	chr8.4:6156863-6156882	MS.gene035718:CDS	50.0%
	TGTTGTCGCGGAGAACCTTA+CGG	+	chr8.4:6156661-6156680	None:intergenic	50.0%
	TGTTTACGCTCTCAAGAGGC+AGG	-	chr8.4:6156862-6156881	MS.gene035718:CDS	50.0%
	CTTGCAAGACGACGAATCGC+AGG	+	chr8.4:6156701-6156720	None:intergenic	55.0%
	GGTTCTCCGCGACAACATTC+AGG	-	chr8.4:6156664-6156683	MS.gene035718:CDS	55.0%
	ACGCTCTCAAGAGGCAGGGA+AGG	-	chr8.4:6156867-6156886	MS.gene035718:CDS	60.0%
	ACGGCGAGCATGCTCTGTGT+AGG	+	chr8.4:6156819-6156838	None:intergenic	60.0%
	CGTCGTCTTGCAAGACGTGG+TGG	-	chr8.4:6156707-6156726	MS.gene035718:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	6156602	6156913	6156602	ID=MS.gene035718
chr8.4	mRNA	6156602	6156913	6156602	ID=MS.gene035718.t1;Parent=MS.gene035718
chr8.4	exon	6156602	6156913	6156602	ID=MS.gene035718.t1.exon1;Parent=MS.gene035718.t1
chr8.4	CDS	6156602	6156913	6156602	ID=cds.MS.gene035718.t1;Parent=MS.gene035718.t1

Gene Sequence	>MS.gene035718 ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTTTAGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTCCGCGACAACATTCAGGGAATTACAAAGCCTGCGATTCGTCGTCTTGCAAGACGTGGTGGTGTGAAACGTATCAGTGGCTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAATGTGATTCGTGATGCTGTCACCTACACAGAGCATGCTCGCCGTAAGACTGTTACTGCGATGGATGTTGTTTACGCTCTCAAGAGGCAGGGAAGGACTCTTTACGGATTCGGTGGTTAG
Protein sequence	>MS.gene035718.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG