AlfalfaGEDB | MS.gene04576

M. sativa cultivar XinJiangDaYe / MS.gene04576

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04576.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04576.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04576.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene88680	MS.gene04576	PPI
MS.gene04576	MS.gene008276	PPI
MS.gene070241	MS.gene04576	PPI
MS.gene056215	MS.gene04576	PPI
MS.gene049821	MS.gene04576	PPI
MS.gene62520	MS.gene04576	PPI
MS.gene04576	MS.gene05619	PPI
MS.gene061876	MS.gene04576	PPI
MS.gene04576	MS.gene89488	PPI
MS.gene05608	MS.gene04576	PPI
MS.gene09871	MS.gene04576	PPI
MS.gene04576	MS.gene61017	PPI
MS.gene060463	MS.gene04576	PPI
MS.gene04576	MS.gene012390	PPI
MS.gene04392	MS.gene04576	PPI
MS.gene30920	MS.gene04576	PPI
MS.gene04068	MS.gene04576	PPI
MS.gene04576	MS.gene016624	PPI
MS.gene04434	MS.gene04576	PPI
MS.gene022946	MS.gene04576	PPI
MS.gene04576	MS.gene73136	PPI
MS.gene04064	MS.gene04576	PPI
MS.gene08121	MS.gene04576	PPI
MS.gene022932	MS.gene04576	PPI
MS.gene012391	MS.gene04576	PPI
MS.gene88681	MS.gene04576	PPI
MS.gene070244	MS.gene04576	PPI
MS.gene035897	MS.gene04576	PPI
MS.gene04576	MS.gene035720	PPI
MS.gene035896	MS.gene04576	PPI
MS.gene73136	MS.gene04576	PPI
MS.gene64920	MS.gene04576	PPI
MS.gene070907	MS.gene04576	PPI
MS.gene009027	MS.gene04576	PPI
MS.gene04576	MS.gene049705	PPI
MS.gene31196	MS.gene04576	PPI
MS.gene010219	MS.gene04576	PPI
MS.gene04052	MS.gene04576	PPI
MS.gene060465	MS.gene04576	PPI
MS.gene04576	MS.gene010219	PPI
MS.gene05623	MS.gene04576	PPI
MS.gene043967	MS.gene04576	PPI
MS.gene04576	MS.gene05608	PPI
MS.gene04576	MS.gene68545	PPI
MS.gene71215	MS.gene04576	PPI
MS.gene061870	MS.gene04576	PPI
MS.gene04576	MS.gene09873	PPI
MS.gene035718	MS.gene04576	PPI
MS.gene008276	MS.gene04576	PPI
MS.gene071136	MS.gene04576	PPI
MS.gene04576	MS.gene62520	PPI
MS.gene012390	MS.gene04576	PPI
MS.gene09873	MS.gene04576	PPI
MS.gene78578	MS.gene04576	PPI
MS.gene79195	MS.gene04576	PPI
MS.gene035721	MS.gene04576	PPI
MS.gene08122	MS.gene04576	PPI
MS.gene04576	MS.gene004124	PPI
MS.gene048705	MS.gene04576	PPI
MS.gene064557	MS.gene04576	PPI
MS.gene038469	MS.gene04576	PPI
MS.gene84536	MS.gene04576	PPI
MS.gene028629	MS.gene04576	PPI
MS.gene82546	MS.gene04576	PPI
MS.gene04576	MS.gene04052	PPI
MS.gene61017	MS.gene04576	PPI
MS.gene04576	MS.gene84536	PPI
MS.gene04433	MS.gene04576	PPI
MS.gene04452	MS.gene04576	PPI
MS.gene04576	MS.gene061866	PPI
MS.gene04576	MS.gene64650	PPI
MS.gene035787	MS.gene04576	PPI
MS.gene04067	MS.gene04576	PPI
MS.gene04576	MS.gene048705	PPI
MS.gene061866	MS.gene04576	PPI
MS.gene04576	MS.gene60093	PPI
MS.gene04576	MS.gene061874	PPI
MS.gene89487	MS.gene04576	PPI
MS.gene04576	MS.gene061869	PPI
MS.gene035719	MS.gene04576	PPI
MS.gene58854	MS.gene04576	PPI
MS.gene66225	MS.gene04576	PPI
MS.gene70955	MS.gene04576	PPI
MS.gene04576	MS.gene04972	PPI
MS.gene040383	MS.gene04576	PPI
MS.gene04576	MS.gene004121	PPI
MS.gene070242	MS.gene04576	PPI
MS.gene072493	MS.gene04576	PPI
MS.gene035720	MS.gene04576	PPI
MS.gene048746	MS.gene04576	PPI
MS.gene010220	MS.gene04576	PPI
MS.gene08053	MS.gene04576	PPI
MS.gene061872	MS.gene04576	PPI
MS.gene04576	MS.gene018578	PPI
MS.gene04576	MS.gene061867	PPI
MS.gene04576	MS.gene018580	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04576.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04576.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04576.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04576.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04576.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04576.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04576.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04576.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04576.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04576.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04576.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04576.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04576.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04576.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04576.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene04576.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04576.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04576.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04576.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04576.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04576.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04576.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04576.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04576.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04576.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04576.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04576.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 36 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	3.1:+46818553	MS.gene04576:CDS
GGAAGAACCCTCTATGGTTT+CGG	0.433408	3.1:+46818668	MS.gene04576:CDS
AGGCAGGGAAGAACCCTCTA+TGG	0.440512	3.1:+46818662	MS.gene04576:CDS
CTTGCCAATCGACGAATGGC+AGG	0.473855	3.1:-46818482	None:intergenic
CAATCGACGAATGGCAGGCT+TGG	0.478331	3.1:-46818477	None:intergenic
ATCAACCTCCGAAACCATAG+AGG	0.481091	3.1:-46818676	None:intergenic
CTGTTGAAAATGTCAGGTCG+TGG	0.487154	3.1:+46818377	None:intergenic
GGTAAGGGACTCGGAAAGGG+AGG	0.488695	3.1:+46818407	MS.gene04576:CDS
CAAGCCTGCCATTCGTCGAT+TGG	0.489516	3.1:+46818478	MS.gene04576:CDS
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.1:+46818391	MS.gene04576:CDS
GGAGGTAAGGGACTCGGAAA+GGG	0.499726	3.1:+46818404	MS.gene04576:CDS
TGTCTATGCTTTGAAAAGGC+AGG	0.502640	3.1:+46818646	MS.gene04576:CDS
GGGAGGTAAGGGACTCGGAA+AGG	0.518226	3.1:+46818403	MS.gene04576:CDS
AGTTCTTCGTGATAACATCC+AGG	0.522451	3.1:+46818448	MS.gene04576:CDS
GTCTATGCTTTGAAAAGGCA+GGG	0.530460	3.1:+46818647	MS.gene04576:CDS
GGAAAGGGAGGTAAGGGACT+CGG	0.535187	3.1:+46818398	MS.gene04576:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.548959	3.1:+46818383	None:intergenic
GGTGGTGTGAAGAGAATCAG+TGG	0.556654	3.1:+46818509	MS.gene04576:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	3.1:+46818392	MS.gene04576:CDS
AGGGAGGTGCAAAGAGACAT+AGG	0.568952	3.1:+46818423	MS.gene04576:CDS
ATGTTGTCTATGCTTTGAAA+AGG	0.580713	3.1:+46818642	MS.gene04576:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.1:+46818382	None:intergenic
TCTTCTTGCCAATCGACGAA+TGG	0.586064	3.1:-46818486	None:intergenic
TGACATACACTGAGCACGCA+AGG	0.587222	3.1:+46818597	MS.gene04576:CDS
GAGGAAGACTGTGACAGCCA+TGG	0.588212	3.1:+46818619	MS.gene04576:CDS
CGTCGATTGGCAAGAAGAGG+TGG	0.589209	3.1:+46818491	MS.gene04576:CDS
CAAAGCATAGACAACATCCA+TGG	0.589691	3.1:-46818636	None:intergenic
AATCAGTGGATTGATCTATG+AGG	0.599604	3.1:+46818523	MS.gene04576:CDS
TGGCAGGCTTGGTGATACCC+TGG	0.613296	3.1:-46818466	None:intergenic
ATTCGTCGATTGGCAAGAAG+AGG	0.624691	3.1:+46818488	MS.gene04576:CDS
AGAACCCTCTATGGTTTCGG+AGG	0.628880	3.1:+46818671	MS.gene04576:CDS
GTTCTTCGTGATAACATCCA+GGG	0.641996	3.1:+46818449	MS.gene04576:CDS
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.1:+46818386	MS.gene04576:CDS
TTGATCTATGAGGAAACAAG+AGG	0.647520	3.1:+46818533	MS.gene04576:CDS
TCAACCTCCGAAACCATAGA+GGG	0.666233	3.1:-46818675	None:intergenic
CATACACTGAGCACGCAAGG+AGG	0.730442	3.1:+46818600	MS.gene04576:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATGTTGTCTATGCTTTGAAA+AGG	+	chr3.1:46818642-46818661	MS.gene04576:CDS	30.0%
	TTGATCTATGAGGAAACAAG+AGG	+	chr3.1:46818533-46818552	MS.gene04576:CDS	35.0%
!!	AATCAGTGGATTGATCTATG+AGG	+	chr3.1:46818523-46818542	MS.gene04576:CDS	35.0%
	AGGTGTCTTGAAGATCTTCT+TGG	+	chr3.1:46818553-46818572	MS.gene04576:CDS	40.0%
	AGTTCTTCGTGATAACATCC+AGG	+	chr3.1:46818448-46818467	MS.gene04576:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	-	chr3.1:46818639-46818658	None:intergenic	40.0%
	GTTCTTCGTGATAACATCCA+GGG	+	chr3.1:46818449-46818468	MS.gene04576:CDS	40.0%
!!	GTCTATGCTTTGAAAAGGCA+GGG	+	chr3.1:46818647-46818666	MS.gene04576:CDS	40.0%
!!	TGTCTATGCTTTGAAAAGGC+AGG	+	chr3.1:46818646-46818665	MS.gene04576:CDS	40.0%
	GGAAGAACCCTCTATGGTTT+CGG	+	chr3.1:46818668-46818687	MS.gene04576:CDS	45.0%
	TCAACCTCCGAAACCATAGA+GGG	-	chr3.1:46818678-46818697	None:intergenic	45.0%
	TCTTCTTGCCAATCGACGAA+TGG	-	chr3.1:46818489-46818508	None:intergenic	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	+	chr3.1:46818488-46818507	MS.gene04576:CDS	45.0%
	AGAACCCTCTATGGTTTCGG+AGG	+	chr3.1:46818671-46818690	MS.gene04576:CDS	50.0%
	GGTGGTGTGAAGAGAATCAG+TGG	+	chr3.1:46818509-46818528	MS.gene04576:CDS	50.0%
	TGACATACACTGAGCACGCA+AGG	+	chr3.1:46818597-46818616	MS.gene04576:CDS	50.0%
!	AGGGAGGTGCAAAGAGACAT+AGG	+	chr3.1:46818423-46818442	MS.gene04576:CDS	50.0%
	AGGCAGGGAAGAACCCTCTA+TGG	+	chr3.1:46818662-46818681	MS.gene04576:CDS	55.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	+	chr3.1:46818391-46818410	MS.gene04576:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	+	chr3.1:46818386-46818405	MS.gene04576:CDS	55.0%
	CAAGCCTGCCATTCGTCGAT+TGG	+	chr3.1:46818478-46818497	MS.gene04576:CDS	55.0%
	CAATCGACGAATGGCAGGCT+TGG	-	chr3.1:46818480-46818499	None:intergenic	55.0%
	CATACACTGAGCACGCAAGG+AGG	+	chr3.1:46818600-46818619	MS.gene04576:CDS	55.0%
	CTTGCCAATCGACGAATGGC+AGG	-	chr3.1:46818485-46818504	None:intergenic	55.0%
	GAGGAAGACTGTGACAGCCA+TGG	+	chr3.1:46818619-46818638	MS.gene04576:CDS	55.0%
	GGAAAGGGAGGTAAGGGACT+CGG	+	chr3.1:46818398-46818417	MS.gene04576:CDS	55.0%
	GGAGGTAAGGGACTCGGAAA+GGG	+	chr3.1:46818404-46818423	MS.gene04576:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	+	chr3.1:46818392-46818411	MS.gene04576:CDS	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	+	chr3.1:46818491-46818510	MS.gene04576:CDS	55.0%
	GGGAGGTAAGGGACTCGGAA+AGG	+	chr3.1:46818403-46818422	MS.gene04576:CDS	60.0%
	GGTAAGGGACTCGGAAAGGG+AGG	+	chr3.1:46818407-46818426	MS.gene04576:CDS	60.0%
!!	TGGCAGGCTTGGTGATACCC+TGG	-	chr3.1:46818469-46818488	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	46818386	46818697	46818386	ID=MS.gene04576
chr3.1	mRNA	46818386	46818697	46818386	ID=MS.gene04576.t1;Parent=MS.gene04576
chr3.1	exon	46818386	46818697	46818386	ID=MS.gene04576.t1.exon1;Parent=MS.gene04576.t1
chr3.1	CDS	46818386	46818697	46818386	ID=cds.MS.gene04576.t1;Parent=MS.gene04576.t1

Gene Sequence	>MS.gene04576 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTCGGAAAGGGAGGTGCAAAGAGACATAGGAAAGTTCTTCGTGATAACATCCAGGGTATCACCAAGCCTGCCATTCGTCGATTGGCAAGAAGAGGTGGTGTGAAGAGAATCAGTGGATTGATCTATGAGGAAACAAGAGGTGTCTTGAAGATCTTCTTGGAGAATGTCATTCGTGACGCTGTGACATACACTGAGCACGCAAGGAGGAAGACTGTGACAGCCATGGATGTTGTCTATGCTTTGAAAAGGCAGGGAAGAACCCTCTATGGTTTCGGAGGTTGA
Protein sequence	>MS.gene04576.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG