AlfalfaGEDB | MS.gene68545

M. sativa cultivar XinJiangDaYe / MS.gene68545

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68545.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68545.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68545.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene035718	MS.gene68545	PPI
MS.gene66225	MS.gene68545	PPI
MS.gene071136	MS.gene68545	PPI
MS.gene012390	MS.gene68545	PPI
MS.gene09873	MS.gene68545	PPI
MS.gene78578	MS.gene68545	PPI
MS.gene79195	MS.gene68545	PPI
MS.gene035721	MS.gene68545	PPI
MS.gene048705	MS.gene68545	PPI
MS.gene064557	MS.gene68545	PPI
MS.gene038469	MS.gene68545	PPI
MS.gene04972	MS.gene68545	PPI
MS.gene82546	MS.gene68545	PPI
MS.gene61017	MS.gene68545	PPI
MS.gene04433	MS.gene68545	PPI
MS.gene04452	MS.gene68545	PPI
MS.gene64650	MS.gene68545	PPI
MS.gene035787	MS.gene68545	PPI
MS.gene04067	MS.gene68545	PPI
MS.gene061874	MS.gene68545	PPI
MS.gene89487	MS.gene68545	PPI
MS.gene035719	MS.gene68545	PPI
MS.gene58854	MS.gene68545	PPI
MS.gene016624	MS.gene68545	PPI
MS.gene70955	MS.gene68545	PPI
MS.gene68545	MS.gene049705	PPI
MS.gene040383	MS.gene68545	PPI
MS.gene070242	MS.gene68545	PPI
MS.gene072493	MS.gene68545	PPI
MS.gene035720	MS.gene68545	PPI
MS.gene30920	MS.gene68545	PPI
MS.gene048746	MS.gene68545	PPI
MS.gene010220	MS.gene68545	PPI
MS.gene08053	MS.gene68545	PPI
MS.gene061872	MS.gene68545	PPI
MS.gene88680	MS.gene68545	PPI
MS.gene070241	MS.gene68545	PPI
MS.gene056215	MS.gene68545	PPI
MS.gene061869	MS.gene68545	PPI
MS.gene049821	MS.gene68545	PPI
MS.gene022946	MS.gene68545	PPI
MS.gene68545	MS.gene016624	PPI
MS.gene62520	MS.gene68545	PPI
MS.gene018580	MS.gene68545	PPI
MS.gene04064	MS.gene68545	PPI
MS.gene061876	MS.gene68545	PPI
MS.gene05619	MS.gene68545	PPI
MS.gene05608	MS.gene68545	PPI
MS.gene012391	MS.gene68545	PPI
MS.gene049705	MS.gene68545	PPI
MS.gene09871	MS.gene68545	PPI
MS.gene035897	MS.gene68545	PPI
MS.gene04576	MS.gene68545	PPI
MS.gene060463	MS.gene68545	PPI
MS.gene89488	MS.gene68545	PPI
MS.gene04392	MS.gene68545	PPI
MS.gene04068	MS.gene68545	PPI
MS.gene04434	MS.gene68545	PPI
MS.gene08121	MS.gene68545	PPI
MS.gene68545	MS.gene64650	PPI
MS.gene022932	MS.gene68545	PPI
MS.gene68545	MS.gene60093	PPI
MS.gene88681	MS.gene68545	PPI
MS.gene070244	MS.gene68545	PPI
MS.gene035896	MS.gene68545	PPI
MS.gene73136	MS.gene68545	PPI
MS.gene64920	MS.gene68545	PPI
MS.gene008276	MS.gene68545	PPI
MS.gene070907	MS.gene68545	PPI
MS.gene60093	MS.gene68545	PPI
MS.gene004124	MS.gene68545	PPI
MS.gene009027	MS.gene68545	PPI
MS.gene68545	MS.gene04972	PPI
MS.gene018578	MS.gene68545	PPI
MS.gene68545	MS.gene004121	PPI
MS.gene31196	MS.gene68545	PPI
MS.gene010219	MS.gene68545	PPI
MS.gene68545	MS.gene018578	PPI
MS.gene04052	MS.gene68545	PPI
MS.gene08122	MS.gene68545	PPI
MS.gene060465	MS.gene68545	PPI
MS.gene05623	MS.gene68545	PPI
MS.gene043967	MS.gene68545	PPI
MS.gene68545	MS.gene018580	PPI
MS.gene68545	MS.gene004124	PPI
MS.gene061867	MS.gene68545	PPI
MS.gene84536	MS.gene68545	PPI
MS.gene028629	MS.gene68545	PPI
MS.gene68545	MS.gene89488	PPI
MS.gene71215	MS.gene68545	PPI
MS.gene061866	MS.gene68545	PPI
MS.gene061870	MS.gene68545	PPI
MS.gene004121	MS.gene68545	PPI
MS.gene68545	MS.gene061874	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68545.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene68545.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene68545.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene68545.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene68545.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene68545.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene68545.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene68545.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene68545.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene68545.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene68545.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene68545.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene68545.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene68545.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene68545.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene68545.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68545.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene68545.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene68545.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene68545.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene68545.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene68545.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene68545.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene68545.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene68545.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene68545.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene68545.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 40 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGCCAATCGACGAATTGC+AGG	0.295956	3.4:+54445996	None:intergenic
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	3.4:-54445925	MS.gene68545:CDS
GGAAGAACCCTCTATGGTTT+CGG	0.433408	3.4:-54445810	MS.gene68545:CDS
AGGCAGGGAAGAACCCTCTA+TGG	0.440512	3.4:-54445816	MS.gene68545:CDS
GGGAGGTAAGGGACTTGGAA+AGG	0.449159	3.4:-54446075	MS.gene68545:CDS
GGAGGTAAGGGACTTGGAAA+GGG	0.467307	3.4:-54446074	MS.gene68545:CDS
GGAAAGGGAGGTAAGGGACT+TGG	0.470633	3.4:-54446080	MS.gene68545:CDS
TTCAACCTCCGAAACCATAG+AGG	0.481091	3.4:+54445802	None:intergenic
ATTTCCTCTTGGAAAATGTC+AGG	0.482279	3.4:-54446107	None:intergenic
CAATCGACGAATTGCAGGCT+TGG	0.486859	3.4:+54446001	None:intergenic
AGGCCGTGGAAAGGGAGGTA+AGG	0.492819	3.4:-54446087	MS.gene68545:CDS
GGTTCTCCGTGACAACATCC+AGG	0.506274	3.4:-54446030	MS.gene68545:CDS
CAAGCCTGCAATTCGTCGAT+TGG	0.509017	3.4:-54446000	MS.gene68545:CDS
GAAGATCTTCAAGACACCTC+TGG	0.510902	3.4:+54445929	None:intergenic
TGTCTATGCACTCAAAAGGC+AGG	0.525428	3.4:-54445832	MS.gene68545:CDS
AGGTGCAAAGAGGCATAGAA+AGG	0.530485	3.4:-54446051	MS.gene68545:CDS
AAAATGTCAGGCCGTGGAAA+GGG	0.531763	3.4:-54446095	MS.gene68545:CDS
ATGTTGTCTATGCACTCAAA+AGG	0.538317	3.4:-54445836	MS.gene68545:CDS
TGACATACACTGAGCACGCT+AGG	0.543435	3.4:-54445881	MS.gene68545:CDS
TCTTGGAAAATGTCAGGCCG+TGG	0.545322	3.4:-54446101	None:intergenic
GAAAATGTCAGGCCGTGGAA+AGG	0.550606	3.4:-54446096	None:intergenic
GGCCGTGGAAAGGGAGGTAA+GGG	0.552535	3.4:-54446086	MS.gene68545:CDS
GTCCCTTACCTCCCTTTCCA+CGG	0.555891	3.4:+54446084	None:intergenic
GGTAAGGGACTTGGAAAGGG+AGG	0.560743	3.4:-54446071	MS.gene68545:CDS
GGTGGTGTGAAGAGAATCAG+TGG	0.582471	3.4:-54445969	MS.gene68545:CDS
GAGGAAGACTGTGACCGCCA+TGG	0.585556	3.4:-54445859	MS.gene68545:CDS
CGTCGATTGGCAAGAAGAGG+TGG	0.589209	3.4:-54445987	MS.gene68545:CDS
GTCTATGCACTCAAAAGGCA+GGG	0.590557	3.4:-54445831	MS.gene68545:CDS
GAGTGCATAGACAACATCCA+TGG	0.612836	3.4:+54445842	None:intergenic
TTGCAGGCTTGGTGATACCC+TGG	0.613296	3.4:+54446012	None:intergenic
ATTCGTCGATTGGCAAGAAG+AGG	0.624691	3.4:-54445990	MS.gene68545:CDS
AGAACCCTCTATGGTTTCGG+AGG	0.628880	3.4:-54445807	MS.gene68545:CDS
ATGTCAGGCCGTGGAAAGGG+AGG	0.634999	3.4:-54446092	MS.gene68545:CDS
TGATACCCTGGATGTTGTCA+CGG	0.654870	3.4:+54446024	None:intergenic
TCAACCTCCGAAACCATAGA+GGG	0.663751	3.4:+54445803	None:intergenic
GTTCTCCGTGACAACATCCA+GGG	0.666413	3.4:-54446029	MS.gene68545:CDS
CATACACTGAGCACGCTAGG+AGG	0.675420	3.4:-54445878	MS.gene68545:CDS
TTGGAAAGGGAGGTGCAAAG+AGG	0.683014	3.4:-54446061	MS.gene68545:CDS
TTGATATATGAAGAAACCAG+AGG	0.683025	3.4:-54445945	MS.gene68545:CDS
TGCATAGACAACATCCATGG+CGG	0.733443	3.4:+54445845	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATATATGAAGAAACCAG+AGG	-	chr3.4:54445950-54445969	MS.gene68545:CDS	30.0%
	ATGTTGTCTATGCACTCAAA+AGG	-	chr3.4:54446059-54446078	MS.gene68545:CDS	35.0%
	AGGTGTCTTGAAGATCTTCT+TGG	-	chr3.4:54445970-54445989	MS.gene68545:CDS	40.0%
	GAAGATCTTCAAGACACCTC+TGG	+	chr3.4:54445969-54445988	None:intergenic	45.0%
	GAGTGCATAGACAACATCCA+TGG	+	chr3.4:54446056-54446075	None:intergenic	45.0%
	GGAAGAACCCTCTATGGTTT+CGG	-	chr3.4:54446085-54446104	MS.gene68545:CDS	45.0%
	GTCTATGCACTCAAAAGGCA+GGG	-	chr3.4:54446064-54446083	MS.gene68545:CDS	45.0%
	TCAACCTCCGAAACCATAGA+GGG	+	chr3.4:54446095-54446114	None:intergenic	45.0%
	TGATACCCTGGATGTTGTCA+CGG	+	chr3.4:54445874-54445893	None:intergenic	45.0%
	TGCATAGACAACATCCATGG+CGG	+	chr3.4:54446053-54446072	None:intergenic	45.0%
	TGTCTATGCACTCAAAAGGC+AGG	-	chr3.4:54446063-54446082	MS.gene68545:CDS	45.0%
!	AGGTGCAAAGAGGCATAGAA+AGG	-	chr3.4:54445844-54445863	MS.gene68545:CDS	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	-	chr3.4:54445905-54445924	MS.gene68545:CDS	45.0%
	AGAACCCTCTATGGTTTCGG+AGG	-	chr3.4:54446088-54446107	MS.gene68545:CDS	50.0%
	CAAGCCTGCAATTCGTCGAT+TGG	-	chr3.4:54445895-54445914	MS.gene68545:CDS	50.0%
	CAATCGACGAATTGCAGGCT+TGG	+	chr3.4:54445897-54445916	None:intergenic	50.0%
	CTTGCCAATCGACGAATTGC+AGG	+	chr3.4:54445902-54445921	None:intergenic	50.0%
	GGAGGTAAGGGACTTGGAAA+GGG	-	chr3.4:54445821-54445840	MS.gene68545:CDS	50.0%
	GGTGGTGTGAAGAGAATCAG+TGG	-	chr3.4:54445926-54445945	MS.gene68545:CDS	50.0%
	GTTCTCCGTGACAACATCCA+GGG	-	chr3.4:54445866-54445885	MS.gene68545:CDS	50.0%
	TGACATACACTGAGCACGCT+AGG	-	chr3.4:54446014-54446033	MS.gene68545:CDS	50.0%
!	TTGGAAAGGGAGGTGCAAAG+AGG	-	chr3.4:54445834-54445853	MS.gene68545:CDS	50.0%
	AGGCAGGGAAGAACCCTCTA+TGG	-	chr3.4:54446079-54446098	MS.gene68545:CDS	55.0%
	CATACACTGAGCACGCTAGG+AGG	-	chr3.4:54446017-54446036	MS.gene68545:CDS	55.0%
	GGAAAGGGAGGTAAGGGACT+TGG	-	chr3.4:54445815-54445834	MS.gene68545:CDS	55.0%
	GGGAGGTAAGGGACTTGGAA+AGG	-	chr3.4:54445820-54445839	MS.gene68545:CDS	55.0%
	GGTAAGGGACTTGGAAAGGG+AGG	-	chr3.4:54445824-54445843	MS.gene68545:CDS	55.0%
	GGTTCTCCGTGACAACATCC+AGG	-	chr3.4:54445865-54445884	MS.gene68545:CDS	55.0%
	GTCCCTTACCTCCCTTTCCA+CGG	+	chr3.4:54445814-54445833	None:intergenic	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	-	chr3.4:54445908-54445927	MS.gene68545:CDS	55.0%
!!	TTGCAGGCTTGGTGATACCC+TGG	+	chr3.4:54445886-54445905	None:intergenic	55.0%
	AGGCCGTGGAAAGGGAGGTA+AGG	-	chr3.4:54445808-54445827	MS.gene68545:CDS	60.0%
	ATGTCAGGCCGTGGAAAGGG+AGG	-	chr3.4:54445803-54445822	MS.gene68545:CDS	60.0%
	GAGGAAGACTGTGACCGCCA+TGG	-	chr3.4:54446036-54446055	MS.gene68545:CDS	60.0%
	GGCCGTGGAAAGGGAGGTAA+GGG	-	chr3.4:54445809-54445828	MS.gene68545:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	54445803	54446114	54445803	ID=MS.gene68545
chr3.4	mRNA	54445803	54446114	54445803	ID=MS.gene68545.t1;Parent=MS.gene68545
chr3.4	exon	54445803	54446114	54445803	ID=MS.gene68545.t1.exon1;Parent=MS.gene68545.t1
chr3.4	CDS	54445803	54446114	54445803	ID=cds.MS.gene68545.t1;Parent=MS.gene68545.t1

Gene Sequence	>MS.gene68545 ATGTCAGGCCGTGGAAAGGGAGGTAAGGGACTTGGAAAGGGAGGTGCAAAGAGGCATAGAAAGGTTCTCCGTGACAACATCCAGGGTATCACCAAGCCTGCAATTCGTCGATTGGCAAGAAGAGGTGGTGTGAAGAGAATCAGTGGTTTGATATATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAATGTCATTCGTGATGCTGTGACATACACTGAGCACGCTAGGAGGAAGACTGTGACCGCCATGGATGTTGTCTATGCACTCAAAAGGCAGGGAAGAACCCTCTATGGTTTCGGAGGTTGA
Protein sequence	>MS.gene68545.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG