AlfalfaGEDB | MS.gene62520

M. sativa cultivar XinJiangDaYe / MS.gene62520

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62520.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62520.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62520.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene31196	MS.gene62520	PPI
MS.gene62520	MS.gene048705	PPI
MS.gene62520	MS.gene61017	PPI
MS.gene010219	MS.gene62520	PPI
MS.gene62520	MS.gene061874	PPI
MS.gene62520	MS.gene04576	PPI
MS.gene060465	MS.gene62520	PPI
MS.gene62520	MS.gene012390	PPI
MS.gene08121	MS.gene62520	PPI
MS.gene62520	MS.gene016624	PPI
MS.gene62520	MS.gene008276	PPI
MS.gene62520	MS.gene73136	PPI
MS.gene035718	MS.gene62520	PPI
MS.gene035896	MS.gene62520	PPI
MS.gene73136	MS.gene62520	PPI
MS.gene008276	MS.gene62520	PPI
MS.gene009027	MS.gene62520	PPI
MS.gene09873	MS.gene62520	PPI
MS.gene62520	MS.gene035720	PPI
MS.gene78578	MS.gene62520	PPI
MS.gene035721	MS.gene62520	PPI
MS.gene04052	MS.gene62520	PPI
MS.gene08122	MS.gene62520	PPI
MS.gene048705	MS.gene62520	PPI
MS.gene05623	MS.gene62520	PPI
MS.gene043967	MS.gene62520	PPI
MS.gene62520	MS.gene049705	PPI
MS.gene84536	MS.gene62520	PPI
MS.gene028629	MS.gene62520	PPI
MS.gene82546	MS.gene62520	PPI
MS.gene04433	MS.gene62520	PPI
MS.gene62520	MS.gene010219	PPI
MS.gene62520	MS.gene05608	PPI
MS.gene04067	MS.gene62520	PPI
MS.gene71215	MS.gene62520	PPI
MS.gene62520	MS.gene68545	PPI
MS.gene061866	MS.gene62520	PPI
MS.gene061870	MS.gene62520	PPI
MS.gene035719	MS.gene62520	PPI
MS.gene58854	MS.gene62520	PPI
MS.gene66225	MS.gene62520	PPI
MS.gene70955	MS.gene62520	PPI
MS.gene071136	MS.gene62520	PPI
MS.gene040383	MS.gene62520	PPI
MS.gene070242	MS.gene62520	PPI
MS.gene072493	MS.gene62520	PPI
MS.gene62520	MS.gene09873	PPI
MS.gene035720	MS.gene62520	PPI
MS.gene012390	MS.gene62520	PPI
MS.gene048746	MS.gene62520	PPI
MS.gene010220	MS.gene62520	PPI
MS.gene79195	MS.gene62520	PPI
MS.gene88680	MS.gene62520	PPI
MS.gene056215	MS.gene62520	PPI
MS.gene064557	MS.gene62520	PPI
MS.gene049821	MS.gene62520	PPI
MS.gene038469	MS.gene62520	PPI
MS.gene61017	MS.gene62520	PPI
MS.gene04452	MS.gene62520	PPI
MS.gene035787	MS.gene62520	PPI
MS.gene09871	MS.gene62520	PPI
MS.gene04576	MS.gene62520	PPI
MS.gene89487	MS.gene62520	PPI
MS.gene62520	MS.gene84536	PPI
MS.gene62520	MS.gene061866	PPI
MS.gene62520	MS.gene64650	PPI
MS.gene62520	MS.gene60093	PPI
MS.gene04392	MS.gene62520	PPI
MS.gene30920	MS.gene62520	PPI
MS.gene62520	MS.gene061869	PPI
MS.gene08053	MS.gene62520	PPI
MS.gene061872	MS.gene62520	PPI
MS.gene04068	MS.gene62520	PPI
MS.gene070241	MS.gene62520	PPI
MS.gene04434	MS.gene62520	PPI
MS.gene62520	MS.gene04972	PPI
MS.gene62520	MS.gene004121	PPI
MS.gene022946	MS.gene62520	PPI
MS.gene62520	MS.gene018578	PPI
MS.gene04064	MS.gene62520	PPI
MS.gene62520	MS.gene061867	PPI
MS.gene061876	MS.gene62520	PPI
MS.gene62520	MS.gene018580	PPI
MS.gene05608	MS.gene62520	PPI
MS.gene62520	MS.gene004124	PPI
MS.gene022932	MS.gene62520	PPI
MS.gene012391	MS.gene62520	PPI
MS.gene88681	MS.gene62520	PPI
MS.gene62520	MS.gene05619	PPI
MS.gene070244	MS.gene62520	PPI
MS.gene035897	MS.gene62520	PPI
MS.gene64920	MS.gene62520	PPI
MS.gene060463	MS.gene62520	PPI
MS.gene62520	MS.gene04052	PPI
MS.gene070907	MS.gene62520	PPI
MS.gene62520	MS.gene89488	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62520.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene62520.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene62520.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene62520.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene62520.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene62520.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene62520.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene62520.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene62520.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene62520.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene62520.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene62520.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene62520.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene62520.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene62520.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene62520.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62520.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene62520.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene62520.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene62520.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene62520.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene62520.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene62520.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene62520.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene62520.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene62520.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene62520.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 29 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCGCCAATCGACGAATTGC+TGG	0.286958	7.3:+70313559	None:intergenic
AGGAGTGTTGAAGATCTTCT+TGG	0.386764	7.3:-70313488	MS.gene62520:CDS
TTGCTGGCTTTGTGATTCCC+TGG	0.413302	7.3:+70313575	None:intergenic
GGAAAGGGAGGTAAAGGATT+AGG	0.430594	7.3:-70313643	MS.gene62520:CDS
AGACAGGGAAGAACCCTCTA+TGG	0.440459	7.3:-70313379	MS.gene62520:CDS
TGTTTATGCGCTTAAGAGAC+AGG	0.444452	7.3:-70313395	MS.gene62520:CDS
GGAAGAACCCTCTATGGTTT+CGG	0.453170	7.3:-70313373	MS.gene62520:CDS
TTCAACCACCGAAACCATAG+AGG	0.495992	7.3:+70313365	None:intergenic
AAAATGTCTGGTCGTGGAAA+GGG	0.502849	7.3:-70313658	MS.gene62520:CDS
GGTTCTCCGCGACAACATCC+AGG	0.511382	7.3:-70313593	MS.gene62520:CDS
AAAGCCAGCAATTCGTCGAT+TGG	0.524127	7.3:-70313563	MS.gene62520:CDS
CGTCGATTGGCGAGAAGAGG+AGG	0.528177	7.3:-70313550	MS.gene62520:CDS
GGTCGTGGAAAGGGAGGTAA+AGG	0.529255	7.3:-70313649	MS.gene62520:CDS
AAGGAAGACTGTGACTGCTA+TGG	0.530595	7.3:-70313422	MS.gene62520:CDS
GGAGGTAAAGGATTAGGAAA+GGG	0.542976	7.3:-70313637	MS.gene62520:CDS
GGGAGGTAAAGGATTAGGAA+AGG	0.547562	7.3:-70313638	MS.gene62520:CDS
ATTCGTCGATTGGCGAGAAG+AGG	0.562203	7.3:-70313553	MS.gene62520:CDS
AATCAGCGGTTTGATCTATG+AGG	0.565644	7.3:-70313518	MS.gene62520:CDS
GGTAAAGGATTAGGAAAGGG+AGG	0.605777	7.3:-70313634	MS.gene62520:CDS
AGAACCCTCTATGGTTTCGG+TGG	0.607847	7.3:-70313370	MS.gene62520:CDS
TGATTCCCTGGATGTTGTCG+CGG	0.639365	7.3:+70313587	None:intergenic
ATGTCTGGTCGTGGAAAGGG+AGG	0.640958	7.3:-70313655	MS.gene62520:CDS
TTGATCTATGAGGAAACAAG+AGG	0.641491	7.3:-70313508	MS.gene62520:CDS
GTTCTCCGCGACAACATCCA+GGG	0.642976	7.3:-70313592	MS.gene62520:CDS
GTTTATGCGCTTAAGAGACA+GGG	0.645697	7.3:-70313394	MS.gene62520:CDS
CTTACACTGAGCATGCGAGA+AGG	0.657279	7.3:-70313441	MS.gene62520:CDS
AGGTGCAAAGAGACATCGCA+AGG	0.665418	7.3:-70313614	MS.gene62520:CDS
TCAACCACCGAAACCATAGA+GGG	0.678652	7.3:+70313366	None:intergenic
GGAGGTGTGAAAAGAATCAG+CGG	0.710972	7.3:-70313532	MS.gene62520:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATCTATGAGGAAACAAG+AGG	-	chr7.3:70313513-70313532	MS.gene62520:CDS	35.0%
	AATCAGCGGTTTGATCTATG+AGG	-	chr7.3:70313503-70313522	MS.gene62520:CDS	40.0%
	GGAGGTAAAGGATTAGGAAA+GGG	-	chr7.3:70313384-70313403	MS.gene62520:CDS	40.0%
	GTTTATGCGCTTAAGAGACA+GGG	-	chr7.3:70313627-70313646	MS.gene62520:CDS	40.0%
	TGTTTATGCGCTTAAGAGAC+AGG	-	chr7.3:70313626-70313645	MS.gene62520:CDS	40.0%
!!	AGGAGTGTTGAAGATCTTCT+TGG	-	chr7.3:70313533-70313552	MS.gene62520:CDS	40.0%
	AAAGCCAGCAATTCGTCGAT+TGG	-	chr7.3:70313458-70313477	MS.gene62520:CDS	45.0%
	AAGGAAGACTGTGACTGCTA+TGG	-	chr7.3:70313599-70313618	MS.gene62520:CDS	45.0%
	GGAAAGGGAGGTAAAGGATT+AGG	-	chr7.3:70313378-70313397	MS.gene62520:CDS	45.0%
	GGAAGAACCCTCTATGGTTT+CGG	-	chr7.3:70313648-70313667	MS.gene62520:CDS	45.0%
	GGGAGGTAAAGGATTAGGAA+AGG	-	chr7.3:70313383-70313402	MS.gene62520:CDS	45.0%
	GGTAAAGGATTAGGAAAGGG+AGG	-	chr7.3:70313387-70313406	MS.gene62520:CDS	45.0%
	TCAACCACCGAAACCATAGA+GGG	+	chr7.3:70313658-70313677	None:intergenic	45.0%
!	GGAGGTGTGAAAAGAATCAG+CGG	-	chr7.3:70313489-70313508	MS.gene62520:CDS	45.0%
	AGAACCCTCTATGGTTTCGG+TGG	-	chr7.3:70313651-70313670	MS.gene62520:CDS	50.0%
	AGACAGGGAAGAACCCTCTA+TGG	-	chr7.3:70313642-70313661	MS.gene62520:CDS	50.0%
	AGGTGCAAAGAGACATCGCA+AGG	-	chr7.3:70313407-70313426	MS.gene62520:CDS	50.0%
	CTTACACTGAGCATGCGAGA+AGG	-	chr7.3:70313580-70313599	MS.gene62520:CDS	50.0%
	TGATTCCCTGGATGTTGTCG+CGG	+	chr7.3:70313437-70313456	None:intergenic	50.0%
!	ATTCGTCGATTGGCGAGAAG+AGG	-	chr7.3:70313468-70313487	MS.gene62520:CDS	50.0%
!!	TTGCTGGCTTTGTGATTCCC+TGG	+	chr7.3:70313449-70313468	None:intergenic	50.0%
	ATGTCTGGTCGTGGAAAGGG+AGG	-	chr7.3:70313366-70313385	MS.gene62520:CDS	55.0%
	CTCGCCAATCGACGAATTGC+TGG	+	chr7.3:70313465-70313484	None:intergenic	55.0%
	GGTCGTGGAAAGGGAGGTAA+AGG	-	chr7.3:70313372-70313391	MS.gene62520:CDS	55.0%
	GTTCTCCGCGACAACATCCA+GGG	-	chr7.3:70313429-70313448	MS.gene62520:CDS	55.0%
	GGTTCTCCGCGACAACATCC+AGG	-	chr7.3:70313428-70313447	MS.gene62520:CDS	60.0%
!	CGTCGATTGGCGAGAAGAGG+AGG	-	chr7.3:70313471-70313490	MS.gene62520:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	70313366	70313677	70313366	ID=MS.gene62520
chr7.3	mRNA	70313366	70313677	70313366	ID=MS.gene62520.t1;Parent=MS.gene62520
chr7.3	exon	70313366	70313677	70313366	ID=MS.gene62520.t1.exon1;Parent=MS.gene62520.t1
chr7.3	CDS	70313366	70313677	70313366	ID=cds.MS.gene62520.t1;Parent=MS.gene62520.t1

Gene Sequence	>MS.gene62520 ATGTCTGGTCGTGGAAAGGGAGGTAAAGGATTAGGAAAGGGAGGTGCAAAGAGACATCGCAAGGTTCTCCGCGACAACATCCAGGGAATCACAAAGCCAGCAATTCGTCGATTGGCGAGAAGAGGAGGTGTGAAAAGAATCAGCGGTTTGATCTATGAGGAAACAAGAGGAGTGTTGAAGATCTTCTTGGAGAATGTGATAAGAGATGCTGTTACTTACACTGAGCATGCGAGAAGGAAGACTGTGACTGCTATGGATGTTGTTTATGCGCTTAAGAGACAGGGAAGAACCCTCTATGGTTTCGGTGGTTGA
Protein sequence	>MS.gene62520.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG