AlfalfaGEDB | MS.gene064557

M. sativa cultivar XinJiangDaYe / MS.gene064557

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064557.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064557.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064557.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049821	MS.gene064557	0.838655	2.53E-57	-1.69E-46
MS.gene050901	MS.gene064557	0.838909	2.17E-57	-1.69E-46
MS.gene051247	MS.gene064557	0.817928	2.56E-52	-1.69E-46
MS.gene051700	MS.gene064557	0.815332	9.76E-52	-1.69E-46
MS.gene051719	MS.gene064557	0.803372	3.60E-49	-1.69E-46
MS.gene053581	MS.gene064557	0.804809	1.81E-49	-1.69E-46
MS.gene055465	MS.gene064557	0.823319	1.48E-53	-1.69E-46
MS.gene055466	MS.gene064557	0.825258	5.17E-54	-1.69E-46
MS.gene055468	MS.gene064557	0.806426	8.27E-50	-1.69E-46
MS.gene055537	MS.gene064557	0.801007	1.10E-48	-1.69E-46
MS.gene056215	MS.gene064557	0.824565	7.53E-54	-1.69E-46
MS.gene059625	MS.gene064557	0.807474	4.97E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene064557	MS.gene66225	PPI
MS.gene064557	MS.gene035720	PPI
MS.gene064557	MS.gene70955	PPI
MS.gene064557	MS.gene61017	PPI
MS.gene064557	MS.gene09871	PPI
MS.gene064557	MS.gene012390	PPI
MS.gene064557	MS.gene08121	PPI
MS.gene056215	MS.gene064557	PPI
MS.gene064557	MS.gene049705	PPI
MS.gene038469	MS.gene064557	PPI
MS.gene064557	MS.gene04434	PPI
MS.gene064557	MS.gene070907	PPI
MS.gene064557	MS.gene04392	PPI
MS.gene04452	MS.gene064557	PPI
MS.gene064557	MS.gene010219	PPI
MS.gene064557	MS.gene05608	PPI
MS.gene064557	MS.gene040383	PPI
MS.gene064557	MS.gene68545	PPI
MS.gene035787	MS.gene064557	PPI
MS.gene064557	MS.gene070242	PPI
MS.gene064557	MS.gene016624	PPI
MS.gene89487	MS.gene064557	PPI
MS.gene064557	MS.gene048746	PPI
MS.gene064557	MS.gene09873	PPI
MS.gene30920	MS.gene064557	PPI
MS.gene08053	MS.gene064557	PPI
MS.gene061872	MS.gene064557	PPI
MS.gene064557	MS.gene043967	PPI
MS.gene88680	MS.gene064557	PPI
MS.gene070241	MS.gene064557	PPI
MS.gene064557	MS.gene035897	PPI
MS.gene064557	MS.gene035721	PPI
MS.gene061876	MS.gene064557	PPI
MS.gene012391	MS.gene064557	PPI
MS.gene88681	MS.gene064557	PPI
MS.gene070244	MS.gene064557	PPI
MS.gene064557	MS.gene072493	PPI
MS.gene64920	MS.gene064557	PPI
MS.gene064557	MS.gene84536	PPI
MS.gene064557	MS.gene035896	PPI
MS.gene060463	MS.gene064557	PPI
MS.gene064557	MS.gene82546	PPI
MS.gene31196	MS.gene064557	PPI
MS.gene064557	MS.gene60093	PPI
MS.gene064557	MS.gene08122	PPI
MS.gene060465	MS.gene064557	PPI
MS.gene064557	MS.gene022932	PPI
MS.gene064557	MS.gene010220	PPI
MS.gene064557	MS.gene04972	PPI
MS.gene064557	MS.gene004121	PPI
MS.gene064557	MS.gene62520	PPI
MS.gene064557	MS.gene018578	PPI
MS.gene08121	MS.gene064557	PPI
MS.gene064557	MS.gene04064	PPI
MS.gene064557	MS.gene018580	PPI
MS.gene064557	MS.gene004124	PPI
MS.gene064557	MS.gene05619	PPI
MS.gene035718	MS.gene064557	PPI
MS.gene064557	MS.gene04052	PPI
MS.gene064557	MS.gene061866	PPI
MS.gene009027	MS.gene064557	PPI
MS.gene064557	MS.gene64650	PPI
MS.gene064557	MS.gene79195	PPI
MS.gene064557	MS.gene048705	PPI
MS.gene064557	MS.gene061874	PPI
MS.gene064557	MS.gene04576	PPI
MS.gene78578	MS.gene064557	PPI
MS.gene064557	MS.gene061869	PPI
MS.gene028629	MS.gene064557	PPI
MS.gene064557	MS.gene04452	PPI
MS.gene04433	MS.gene064557	PPI
MS.gene064557	MS.gene061867	PPI
MS.gene04067	MS.gene064557	PPI
MS.gene71215	MS.gene064557	PPI
MS.gene061870	MS.gene064557	PPI
MS.gene064557	MS.gene008276	PPI
MS.gene064557	MS.gene73136	PPI
MS.gene035719	MS.gene064557	PPI
MS.gene064557	MS.gene049821	PPI
MS.gene58854	MS.gene064557	PPI
MS.gene064557	MS.gene04068	PPI
MS.gene064557	MS.gene022946	PPI
MS.gene064557	MS.gene05623	PPI
MS.gene064557	MS.gene89488	PPI
MS.gene70955	MS.gene064557	PPI
MS.gene071136	MS.gene064557	PPI
MS.gene040383	MS.gene064557	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064557.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene064557.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene064557.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene064557.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene064557.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene064557.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene064557.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene064557.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene064557.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene064557.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene064557.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene064557.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene064557.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene064557.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene064557.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene064557.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064557.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene064557.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene064557.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene064557.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene064557.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene064557.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene064557.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene064557.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene064557.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene064557.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene064557.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 30 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGCAAGGAAGAACGCTTTA+TGG	0.253984	3.1:-41113601	MS.gene064557:CDS
GGAAGAACGCTTTATGGATT+CGG	0.418585	3.1:-41113595	MS.gene064557:CDS
GGGAGGTAAGGGACTAGGAA+AGG	0.428277	3.1:-41113860	MS.gene064557:CDS
CTTGCCAATCGACGAATCGC+TGG	0.435511	3.1:+41113781	None:intergenic
GGAGGTAAGGGACTAGGAAA+GGG	0.452874	3.1:-41113859	MS.gene064557:CDS
CTCTTGAAAATGTCAGGTCG+TGG	0.456977	3.1:-41113886	None:intergenic
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.1:-41113872	MS.gene064557:CDS
AAAGCCAGCGATTCGTCGAT+TGG	0.493824	3.1:-41113785	MS.gene064557:CDS
AGTTCTTCGTGACAACATCC+AGG	0.501637	3.1:-41113815	MS.gene064557:CDS
GGAAAGGGAGGTAAGGGACT+AGG	0.521051	3.1:-41113865	MS.gene064557:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.538992	3.1:-41113880	MS.gene064557:CDS
GAGGAAGACGGTTACAGCAA+TGG	0.550679	3.1:-41113644	MS.gene064557:CDS
GTTTATGCTTTGAAGAGGCA+AGG	0.559764	3.1:-41113616	MS.gene064557:CDS
GGTAAGGGACTAGGAAAGGG+AGG	0.563878	3.1:-41113856	MS.gene064557:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	3.1:-41113871	MS.gene064557:CDS
AATCAGCGGTTTGATCTATG+AGG	0.581523	3.1:-41113740	MS.gene064557:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.1:-41113881	None:intergenic
TCGCTGGCTTTGTGATACCC+TGG	0.588577	3.1:+41113797	None:intergenic
CGTCGATTGGCAAGAAGAGG+TGG	0.589209	3.1:-41113772	MS.gene064557:CDS
TGACATACACTGAGCATGCA+AGG	0.589705	3.1:-41113666	MS.gene064557:CDS
AGAACGCTTTATGGATTCGG+AGG	0.604365	3.1:-41113592	MS.gene064557:CDS
ATTCGTCGATTGGCAAGAAG+AGG	0.625836	3.1:-41113775	MS.gene064557:CDS
TTGATCTATGAGGAAACAAG+AGG	0.641491	3.1:-41113730	MS.gene064557:CDS
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.1:-41113877	MS.gene064557:CDS
TGAGCATGCAAGGAGGAAGA+CGG	0.657376	3.1:-41113656	MS.gene064557:CDS
GGTGGTGTTAAGAGAATCAG+CGG	0.661394	3.1:-41113754	MS.gene064557:CDS
ATGTTGTTTATGCTTTGAAG+AGG	0.665215	3.1:-41113621	MS.gene064557:CDS
GTTCTTCGTGACAACATCCA+GGG	0.666413	3.1:-41113814	MS.gene064557:CDS
GGAGAATGTGATTCGTGACG+CGG	0.710348	3.1:-41113689	MS.gene064557:CDS
CATACACTGAGCATGCAAGG+AGG	0.756152	3.1:-41113663	MS.gene064557:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGGAGTTCTTAAGATTTTCT+TGG	-	chr3.1:41113755-41113774	MS.gene064557:CDS	30.0%
!!	ATGTTGTTTATGCTTTGAAG+AGG	-	chr3.1:41113844-41113863	MS.gene064557:CDS	30.0%
	TTGATCTATGAGGAAACAAG+AGG	-	chr3.1:41113735-41113754	MS.gene064557:CDS	35.0%
	AATCAGCGGTTTGATCTATG+AGG	-	chr3.1:41113725-41113744	MS.gene064557:CDS	40.0%
	GGAAGAACGCTTTATGGATT+CGG	-	chr3.1:41113870-41113889	MS.gene064557:CDS	40.0%
!!	GTTTATGCTTTGAAGAGGCA+AGG	-	chr3.1:41113849-41113868	MS.gene064557:CDS	40.0%
	AGAACGCTTTATGGATTCGG+AGG	-	chr3.1:41113873-41113892	MS.gene064557:CDS	45.0%
	AGGCAAGGAAGAACGCTTTA+TGG	-	chr3.1:41113864-41113883	MS.gene064557:CDS	45.0%
	AGTTCTTCGTGACAACATCC+AGG	-	chr3.1:41113650-41113669	MS.gene064557:CDS	45.0%
	GTTCTTCGTGACAACATCCA+GGG	-	chr3.1:41113651-41113670	MS.gene064557:CDS	45.0%
	TGACATACACTGAGCATGCA+AGG	-	chr3.1:41113799-41113818	MS.gene064557:CDS	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	-	chr3.1:41113690-41113709	MS.gene064557:CDS	45.0%
!	GGTGGTGTTAAGAGAATCAG+CGG	-	chr3.1:41113711-41113730	MS.gene064557:CDS	45.0%
	AAAGCCAGCGATTCGTCGAT+TGG	-	chr3.1:41113680-41113699	MS.gene064557:CDS	50.0%
	CATACACTGAGCATGCAAGG+AGG	-	chr3.1:41113802-41113821	MS.gene064557:CDS	50.0%
	GAGGAAGACGGTTACAGCAA+TGG	-	chr3.1:41113821-41113840	MS.gene064557:CDS	50.0%
	GGAGAATGTGATTCGTGACG+CGG	-	chr3.1:41113776-41113795	MS.gene064557:CDS	50.0%
	GGAGGTAAGGGACTAGGAAA+GGG	-	chr3.1:41113606-41113625	MS.gene064557:CDS	50.0%
	TGAGCATGCAAGGAGGAAGA+CGG	-	chr3.1:41113809-41113828	MS.gene064557:CDS	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	-	chr3.1:41113593-41113612	MS.gene064557:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	-	chr3.1:41113588-41113607	MS.gene064557:CDS	55.0%
	CTTGCCAATCGACGAATCGC+TGG	+	chr3.1:41113687-41113706	None:intergenic	55.0%
	GGAAAGGGAGGTAAGGGACT+AGG	-	chr3.1:41113600-41113619	MS.gene064557:CDS	55.0%
	GGGAGGTAAGGGACTAGGAA+AGG	-	chr3.1:41113605-41113624	MS.gene064557:CDS	55.0%
	GGTAAGGGACTAGGAAAGGG+AGG	-	chr3.1:41113609-41113628	MS.gene064557:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	-	chr3.1:41113594-41113613	MS.gene064557:CDS	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	-	chr3.1:41113693-41113712	MS.gene064557:CDS	55.0%
!!	TCGCTGGCTTTGTGATACCC+TGG	+	chr3.1:41113671-41113690	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	41113588	41113899	41113588	ID=MS.gene064557
chr3.1	mRNA	41113588	41113899	41113588	ID=MS.gene064557.t1;Parent=MS.gene064557
chr3.1	exon	41113588	41113899	41113588	ID=MS.gene064557.t1.exon1;Parent=MS.gene064557.t1
chr3.1	CDS	41113588	41113899	41113588	ID=cds.MS.gene064557.t1;Parent=MS.gene064557.t1

Gene Sequence	>MS.gene064557 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTAGGAAAGGGAGGTGCAAAGAGACATCGAAAAGTTCTTCGTGACAACATCCAGGGTATCACAAAGCCAGCGATTCGTCGATTGGCAAGAAGAGGTGGTGTTAAGAGAATCAGCGGTTTGATCTATGAGGAAACAAGAGGAGTTCTTAAGATTTTCTTGGAGAATGTGATTCGTGACGCGGTGACATACACTGAGCATGCAAGGAGGAAGACGGTTACAGCAATGGATGTTGTTTATGCTTTGAAGAGGCAAGGAAGAACGCTTTATGGATTCGGAGGTTGA
Protein sequence	>MS.gene064557.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG