AlfalfaGEDB | MS.gene040383

M. sativa cultivar XinJiangDaYe / MS.gene040383

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040383.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040383.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040383.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene040383	MS.gene04434	PPI
MS.gene040383	MS.gene04452	PPI
MS.gene071136	MS.gene040383	PPI
MS.gene040383	MS.gene016624	PPI
MS.gene040383	MS.gene73136	PPI
MS.gene040383	MS.gene049821	PPI
MS.gene056215	MS.gene040383	PPI
MS.gene064557	MS.gene040383	PPI
MS.gene038469	MS.gene040383	PPI
MS.gene040383	MS.gene022946	PPI
MS.gene040383	MS.gene05623	PPI
MS.gene04433	MS.gene040383	PPI
MS.gene04452	MS.gene040383	PPI
MS.gene040383	MS.gene035720	PPI
MS.gene040383	MS.gene70955	PPI
MS.gene040383	MS.gene09871	PPI
MS.gene035787	MS.gene040383	PPI
MS.gene89487	MS.gene040383	PPI
MS.gene040383	MS.gene049705	PPI
MS.gene035719	MS.gene040383	PPI
MS.gene040383	MS.gene070907	PPI
MS.gene040383	MS.gene04392	PPI
MS.gene58854	MS.gene040383	PPI
MS.gene040383	MS.gene010219	PPI
MS.gene70955	MS.gene040383	PPI
MS.gene040383	MS.gene05608	PPI
MS.gene30920	MS.gene040383	PPI
MS.gene040383	MS.gene68545	PPI
MS.gene08053	MS.gene040383	PPI
MS.gene061872	MS.gene040383	PPI
MS.gene040383	MS.gene070242	PPI
MS.gene040383	MS.gene82546	PPI
MS.gene88680	MS.gene040383	PPI
MS.gene070241	MS.gene040383	PPI
MS.gene040383	MS.gene048746	PPI
MS.gene040383	MS.gene08122	PPI
MS.gene040383	MS.gene09873	PPI
MS.gene061876	MS.gene040383	PPI
MS.gene040383	MS.gene022932	PPI
MS.gene012391	MS.gene040383	PPI
MS.gene040383	MS.gene043967	PPI
MS.gene070244	MS.gene040383	PPI
MS.gene64920	MS.gene040383	PPI
MS.gene040383	MS.gene62520	PPI
MS.gene060463	MS.gene040383	PPI
MS.gene040383	MS.gene035897	PPI
MS.gene040383	MS.gene035721	PPI
MS.gene31196	MS.gene040383	PPI
MS.gene040383	MS.gene004124	PPI
MS.gene040383	MS.gene072493	PPI
MS.gene040383	MS.gene04052	PPI
MS.gene040383	MS.gene84536	PPI
MS.gene040383	MS.gene035896	PPI
MS.gene040383	MS.gene061866	PPI
MS.gene040383	MS.gene64650	PPI
MS.gene040383	MS.gene60093	PPI
MS.gene040383	MS.gene061874	PPI
MS.gene08121	MS.gene040383	PPI
MS.gene040383	MS.gene061869	PPI
MS.gene88681	MS.gene040383	PPI
MS.gene040383	MS.gene010220	PPI
MS.gene035718	MS.gene040383	PPI
MS.gene040383	MS.gene04972	PPI
MS.gene040383	MS.gene004121	PPI
MS.gene040383	MS.gene018578	PPI
MS.gene009027	MS.gene040383	PPI
MS.gene040383	MS.gene061867	PPI
MS.gene040383	MS.gene04064	PPI
MS.gene040383	MS.gene018580	PPI
MS.gene040383	MS.gene008276	PPI
MS.gene78578	MS.gene040383	PPI
MS.gene040383	MS.gene05619	PPI
MS.gene060465	MS.gene040383	PPI
MS.gene040383	MS.gene04068	PPI
MS.gene040383	MS.gene89488	PPI
MS.gene028629	MS.gene040383	PPI
MS.gene040383	MS.gene66225	PPI
MS.gene040383	MS.gene79195	PPI
MS.gene040383	MS.gene048705	PPI
MS.gene040383	MS.gene61017	PPI
MS.gene040383	MS.gene04576	PPI
MS.gene04067	MS.gene040383	PPI
MS.gene71215	MS.gene040383	PPI
MS.gene040383	MS.gene012390	PPI
MS.gene040383	MS.gene08121	PPI
MS.gene061870	MS.gene040383	PPI
MS.gene040383	MS.gene064557	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040383.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene040383.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene040383.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene040383.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene040383.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene040383.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene040383.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene040383.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene040383.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene040383.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene040383.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene040383.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene040383.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene040383.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene040383.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene040383.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040383.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene040383.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene040383.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene040383.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene040383.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene040383.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene040383.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene040383.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene040383.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene040383.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene040383.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 34 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCGCCAATCGACGAATTGC+TGG	0.286958	7.4:+71896699	None:intergenic
GGGTTCTTCCCTGTCTCTTA+AGG	0.300277	7.4:+71896526	None:intergenic
GGTTCTTCCCTGTCTCTTAA+GGG	0.396932	7.4:+71896527	None:intergenic
GGAAAGGGAGGTAAAGGATT+AGG	0.430594	7.4:-71896783	MS.gene040383:CDS
AGGTGTGTTGAAGATCTTCT+TGG	0.431919	7.4:-71896628	MS.gene040383:CDS
AGACAGGGAAGAACCCTCTA+TGG	0.440459	7.4:-71896519	MS.gene040383:CDS
GGAAGAACCCTCTATGGTTT+CGG	0.453170	7.4:-71896513	MS.gene040383:CDS
TGTTTATGCCCTTAAGAGAC+AGG	0.454713	7.4:-71896535	MS.gene040383:CDS
CTTCTTGGAGAATGTGATAA+GGG	0.464768	7.4:-71896613	MS.gene040383:CDS
CTTCCCAAAATGTCTGGTCG+TGG	0.478662	7.4:-71896804	None:intergenic
TCTTCTTGGAGAATGTGATA+AGG	0.492723	7.4:-71896614	MS.gene040383:CDS
TTCAACCACCGAAACCATAG+AGG	0.495992	7.4:+71896505	None:intergenic
AAAATGTCTGGTCGTGGAAA+GGG	0.499560	7.4:-71896798	MS.gene040383:CDS
AAAGCCAGCAATTCGTCGAT+TGG	0.524127	7.4:-71896703	MS.gene040383:CDS
CGTCGATTGGCGAGAAGAGG+AGG	0.528177	7.4:-71896690	MS.gene040383:CDS
GTTCTCCGCGACAACATTCA+AGG	0.528453	7.4:-71896732	MS.gene040383:CDS
GGTCGTGGAAAGGGAGGTAA+AGG	0.529255	7.4:-71896789	MS.gene040383:CDS
CAAAATGTCTGGTCGTGGAA+AGG	0.529768	7.4:-71896799	None:intergenic
GAGGAAGACTGTGACTGCTA+TGG	0.530595	7.4:-71896562	MS.gene040383:CDS
GGAGGTAAAGGATTAGGAAA+GGG	0.542976	7.4:-71896777	MS.gene040383:CDS
GGGAGGTAAAGGATTAGGAA+AGG	0.547562	7.4:-71896778	MS.gene040383:CDS
ATTCGTCGATTGGCGAGAAG+AGG	0.562203	7.4:-71896693	MS.gene040383:CDS
AATCAGCGGATTGATCTATG+AGG	0.586450	7.4:-71896658	MS.gene040383:CDS
GGTAAAGGATTAGGAAAGGG+AGG	0.605777	7.4:-71896774	MS.gene040383:CDS
AGAACCCTCTATGGTTTCGG+TGG	0.607847	7.4:-71896510	MS.gene040383:CDS
TTACTTACACTGAGCATGCG+AGG	0.631466	7.4:-71896584	MS.gene040383:CDS
ATGTCTGGTCGTGGAAAGGG+AGG	0.640958	7.4:-71896795	MS.gene040383:CDS
GTTTATGCCCTTAAGAGACA+GGG	0.645697	7.4:-71896534	MS.gene040383:CDS
TTGATCTATGAGGAAACAAG+AGG	0.647520	7.4:-71896648	MS.gene040383:CDS
AGGTGCAAAGAGACATCGCA+AGG	0.665418	7.4:-71896754	MS.gene040383:CDS
TGATTCCTTGAATGTTGTCG+CGG	0.666851	7.4:+71896727	None:intergenic
TCAACCACCGAAACCATAGA+GGG	0.678652	7.4:+71896506	None:intergenic
GGAGGTGTGAAAAGAATCAG+CGG	0.699104	7.4:-71896672	MS.gene040383:CDS
CTTACACTGAGCATGCGAGG+AGG	0.750750	7.4:-71896581	MS.gene040383:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTTCTTGGAGAATGTGATAA+GGG	-	chr7.4:71896688-71896707	MS.gene040383:CDS	35.0%
	TCTTCTTGGAGAATGTGATA+AGG	-	chr7.4:71896687-71896706	MS.gene040383:CDS	35.0%
	TTGATCTATGAGGAAACAAG+AGG	-	chr7.4:71896653-71896672	MS.gene040383:CDS	35.0%
	AGGTGTGTTGAAGATCTTCT+TGG	-	chr7.4:71896673-71896692	MS.gene040383:CDS	40.0%
	GGAGGTAAAGGATTAGGAAA+GGG	-	chr7.4:71896524-71896543	MS.gene040383:CDS	40.0%
	GTTTATGCCCTTAAGAGACA+GGG	-	chr7.4:71896767-71896786	MS.gene040383:CDS	40.0%
	TGATTCCTTGAATGTTGTCG+CGG	+	chr7.4:71896577-71896596	None:intergenic	40.0%
	TGTTTATGCCCTTAAGAGAC+AGG	-	chr7.4:71896766-71896785	MS.gene040383:CDS	40.0%
!!	AATCAGCGGATTGATCTATG+AGG	-	chr7.4:71896643-71896662	MS.gene040383:CDS	40.0%
	AAAGCCAGCAATTCGTCGAT+TGG	-	chr7.4:71896598-71896617	MS.gene040383:CDS	45.0%
	GGAAAGGGAGGTAAAGGATT+AGG	-	chr7.4:71896518-71896537	MS.gene040383:CDS	45.0%
	GGAAGAACCCTCTATGGTTT+CGG	-	chr7.4:71896788-71896807	MS.gene040383:CDS	45.0%
	GGGAGGTAAAGGATTAGGAA+AGG	-	chr7.4:71896523-71896542	MS.gene040383:CDS	45.0%
	GGTAAAGGATTAGGAAAGGG+AGG	-	chr7.4:71896527-71896546	MS.gene040383:CDS	45.0%
	GGTTCTTCCCTGTCTCTTAA+GGG	+	chr7.4:71896777-71896796	None:intergenic	45.0%
	TCAACCACCGAAACCATAGA+GGG	+	chr7.4:71896798-71896817	None:intergenic	45.0%
	TTACTTACACTGAGCATGCG+AGG	-	chr7.4:71896717-71896736	MS.gene040383:CDS	45.0%
!	GGAGGTGTGAAAAGAATCAG+CGG	-	chr7.4:71896629-71896648	MS.gene040383:CDS	45.0%
	AGAACCCTCTATGGTTTCGG+TGG	-	chr7.4:71896791-71896810	MS.gene040383:CDS	50.0%
	AGACAGGGAAGAACCCTCTA+TGG	-	chr7.4:71896782-71896801	MS.gene040383:CDS	50.0%
	AGGTGCAAAGAGACATCGCA+AGG	-	chr7.4:71896547-71896566	MS.gene040383:CDS	50.0%
	GAGGAAGACTGTGACTGCTA+TGG	-	chr7.4:71896739-71896758	MS.gene040383:CDS	50.0%
	GGGTTCTTCCCTGTCTCTTA+AGG	+	chr7.4:71896778-71896797	None:intergenic	50.0%
	GTTCTCCGCGACAACATTCA+AGG	-	chr7.4:71896569-71896588	MS.gene040383:CDS	50.0%
!	ATTCGTCGATTGGCGAGAAG+AGG	-	chr7.4:71896608-71896627	MS.gene040383:CDS	50.0%
	ATGTCTGGTCGTGGAAAGGG+AGG	-	chr7.4:71896506-71896525	MS.gene040383:CDS	55.0%
	CTCGCCAATCGACGAATTGC+TGG	+	chr7.4:71896605-71896624	None:intergenic	55.0%
	CTTACACTGAGCATGCGAGG+AGG	-	chr7.4:71896720-71896739	MS.gene040383:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+AGG	-	chr7.4:71896512-71896531	MS.gene040383:CDS	55.0%
!	CGTCGATTGGCGAGAAGAGG+AGG	-	chr7.4:71896611-71896630	MS.gene040383:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	71896506	71896817	71896506	ID=MS.gene040383
chr7.4	mRNA	71896506	71896817	71896506	ID=MS.gene040383.t1;Parent=MS.gene040383
chr7.4	exon	71896506	71896817	71896506	ID=MS.gene040383.t1.exon1;Parent=MS.gene040383.t1
chr7.4	CDS	71896506	71896817	71896506	ID=cds.MS.gene040383.t1;Parent=MS.gene040383.t1

Gene Sequence	>MS.gene040383 ATGTCTGGTCGTGGAAAGGGAGGTAAAGGATTAGGAAAGGGAGGTGCAAAGAGACATCGCAAGGTTCTCCGCGACAACATTCAAGGAATCACAAAGCCAGCAATTCGTCGATTGGCGAGAAGAGGAGGTGTGAAAAGAATCAGCGGATTGATCTATGAGGAAACAAGAGGTGTGTTGAAGATCTTCTTGGAGAATGTGATAAGGGATGCTGTTACTTACACTGAGCATGCGAGGAGGAAGACTGTGACTGCTATGGATGTTGTTTATGCCCTTAAGAGACAGGGAAGAACCCTCTATGGTTTCGGTGGTTGA
Protein sequence	>MS.gene040383.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG