AlfalfaGEDB | MS.gene88681

M. sativa cultivar XinJiangDaYe / MS.gene88681

KEGG: K11253
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88681.t1	KAD4179940.1	96.3	109	4	0	4	112	741	849	8.70E-50	206.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88681.t1	P62785	100.0	103	0	0	10	112	1	103	8.8e-51	200.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88681.t1	A0A2K2CI65	99.1	106	1	0	7	112	93	198	8.2e-50	205.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene88681	0.832663	8.32E-56	-1.69E-46
MS.gene049159	MS.gene88681	0.826375	2.81E-54	-1.69E-46
MS.gene049368	MS.gene88681	0.805879	1.08E-49	-1.69E-46
MS.gene049404	MS.gene88681	0.802923	4.46E-49	-1.69E-46
MS.gene049821	MS.gene88681	0.876187	1.70E-68	-1.69E-46
MS.gene049850	MS.gene88681	0.81724	3.65E-52	-1.69E-46
MS.gene050020	MS.gene88681	0.812051	5.15E-51	-1.69E-46
MS.gene05045	MS.gene88681	0.800227	1.59E-48	-1.69E-46
MS.gene050689	MS.gene88681	0.812753	3.62E-51	-1.69E-46
MS.gene050759	MS.gene88681	0.803983	2.69E-49	-1.69E-46
MS.gene050807	MS.gene88681	0.830951	2.20E-55	-1.69E-46
MS.gene050808	MS.gene88681	0.812533	4.04E-51	-1.69E-46
MS.gene050838	MS.gene88681	0.816248	6.10E-52	-1.69E-46
MS.gene050901	MS.gene88681	0.839025	2.03E-57	-1.69E-46
MS.gene051247	MS.gene88681	0.8193	1.25E-52	-1.69E-46
MS.gene051328	MS.gene88681	0.849768	2.62E-60	-1.69E-46
MS.gene05134	MS.gene88681	0.801464	8.89E-49	-1.69E-46
MS.gene051490	MS.gene88681	0.80286	4.59E-49	-1.69E-46
MS.gene05157	MS.gene88681	0.816393	5.66E-52	-1.69E-46
MS.gene051700	MS.gene88681	0.816549	5.22E-52	-1.69E-46
MS.gene051719	MS.gene88681	0.837477	5.08E-57	-1.69E-46
MS.gene051720	MS.gene88681	0.828034	1.12E-54	-1.69E-46
MS.gene051975	MS.gene88681	0.815063	1.12E-51	-1.69E-46
MS.gene052020	MS.gene88681	0.801569	8.46E-49	-1.69E-46
MS.gene052133	MS.gene88681	0.847402	1.18E-59	-1.69E-46
MS.gene052463	MS.gene88681	0.804299	2.31E-49	-1.69E-46
MS.gene052516	MS.gene88681	0.837283	5.70E-57	-1.69E-46
MS.gene052665	MS.gene88681	0.807208	5.66E-50	-1.69E-46
MS.gene052947	MS.gene88681	0.80226	6.11E-49	-1.69E-46
MS.gene053008	MS.gene88681	0.80808	3.69E-50	-1.69E-46
MS.gene053079	MS.gene88681	0.810473	1.13E-50	-1.69E-46
MS.gene053226	MS.gene88681	0.830173	3.41E-55	-1.69E-46
MS.gene053563	MS.gene88681	0.803093	4.11E-49	-1.69E-46
MS.gene053581	MS.gene88681	0.833547	5.01E-56	-1.69E-46
MS.gene05364	MS.gene88681	0.808015	3.81E-50	-1.69E-46
MS.gene05458	MS.gene88681	0.800353	1.50E-48	-1.69E-46
MS.gene055465	MS.gene88681	0.832048	1.18E-55	-1.69E-46
MS.gene055466	MS.gene88681	0.842421	2.61E-58	-1.69E-46
MS.gene055468	MS.gene88681	0.84688	1.64E-59	-1.69E-46
MS.gene055469	MS.gene88681	0.830863	2.31E-55	-1.69E-46
MS.gene055513	MS.gene88681	0.826029	3.39E-54	-1.69E-46
MS.gene055608	MS.gene88681	0.802691	4.98E-49	-1.69E-46
MS.gene055778	MS.gene88681	0.802015	6.85E-49	-1.69E-46
MS.gene055818	MS.gene88681	0.80681	6.87E-50	-1.69E-46
MS.gene055926	MS.gene88681	0.810819	9.54E-51	-1.69E-46
MS.gene05608	MS.gene88681	0.800909	1.15E-48	-1.69E-46
MS.gene056174	MS.gene88681	0.832484	9.21E-56	-1.69E-46
MS.gene056215	MS.gene88681	0.852968	3.26E-61	-1.69E-46
MS.gene056308	MS.gene88681	0.826116	3.24E-54	-1.69E-46
MS.gene056595	MS.gene88681	0.802928	4.44E-49	-1.69E-46
MS.gene057693	MS.gene88681	0.855297	6.93E-62	-1.69E-46
MS.gene057915	MS.gene88681	0.825651	4.17E-54	-1.69E-46
MS.gene058010	MS.gene88681	0.814929	1.20E-51	-1.69E-46
MS.gene05847	MS.gene88681	0.827254	1.73E-54	-1.69E-46
MS.gene059170	MS.gene88681	0.810508	1.11E-50	-1.69E-46
MS.gene05978	MS.gene88681	0.804581	2.02E-49	-1.69E-46
MS.gene059842	MS.gene88681	0.831107	2.01E-55	-1.69E-46
MS.gene059937	MS.gene88681	0.805069	1.59E-49	-1.69E-46
MS.gene060429	MS.gene88681	0.820865	5.47E-53	-1.69E-46
MS.gene060587	MS.gene88681	0.825461	4.63E-54	-1.69E-46
MS.gene060803	MS.gene88681	0.831101	2.02E-55	-1.69E-46
MS.gene060917	MS.gene88681	0.808166	3.54E-50	-1.69E-46
MS.gene061006	MS.gene88681	0.804871	1.75E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene88681	MS.gene072493	PPI
MS.gene88681	MS.gene84536	PPI
MS.gene88681	MS.gene035896	PPI
MS.gene88681	MS.gene061866	PPI
MS.gene035718	MS.gene88681	PPI
MS.gene88681	MS.gene64650	PPI
MS.gene88681	MS.gene60093	PPI
MS.gene009027	MS.gene88681	PPI
MS.gene88681	MS.gene061869	PPI
MS.gene78578	MS.gene88681	PPI
MS.gene88681	MS.gene010220	PPI
MS.gene060465	MS.gene88681	PPI
MS.gene88681	MS.gene04972	PPI
MS.gene88681	MS.gene004121	PPI
MS.gene88681	MS.gene018578	PPI
MS.gene028629	MS.gene88681	PPI
MS.gene88681	MS.gene061867	PPI
MS.gene88681	MS.gene04064	PPI
MS.gene88681	MS.gene018580	PPI
MS.gene88681	MS.gene004124	PPI
MS.gene88681	MS.gene05619	PPI
MS.gene04067	MS.gene88681	PPI
MS.gene71215	MS.gene88681	PPI
MS.gene061870	MS.gene88681	PPI
MS.gene88681	MS.gene04068	PPI
MS.gene88681	MS.gene04052	PPI
MS.gene88681	MS.gene89488	PPI
MS.gene88681	MS.gene66225	PPI
MS.gene88681	MS.gene79195	PPI
MS.gene88681	MS.gene048705	PPI
MS.gene88681	MS.gene61017	PPI
MS.gene88681	MS.gene061874	PPI
MS.gene071136	MS.gene88681	PPI
MS.gene88681	MS.gene04576	PPI
MS.gene88681	MS.gene012390	PPI
MS.gene88681	MS.gene08121	PPI
MS.gene88681	MS.gene64920	PPI
MS.gene88681	MS.gene064557	PPI
MS.gene88681	MS.gene04434	PPI
MS.gene88681	MS.gene04452	PPI
MS.gene88681	MS.gene89487	PPI
MS.gene056215	MS.gene88681	PPI
MS.gene88681	MS.gene04433	PPI
MS.gene88681	MS.gene038469	PPI
MS.gene04433	MS.gene88681	PPI
MS.gene88681	MS.gene016624	PPI
MS.gene88681	MS.gene008276	PPI
MS.gene035787	MS.gene88681	PPI
MS.gene88681	MS.gene73136	PPI
MS.gene88681	MS.gene049821	PPI
MS.gene89487	MS.gene88681	PPI
MS.gene88681	MS.gene022946	PPI
MS.gene88681	MS.gene035719	PPI
MS.gene88681	MS.gene05623	PPI
MS.gene58854	MS.gene88681	PPI
MS.gene88681	MS.gene58854	PPI
MS.gene88681	MS.gene035720	PPI
MS.gene88681	MS.gene70955	PPI
MS.gene88681	MS.gene09871	PPI
MS.gene30920	MS.gene88681	PPI
MS.gene061872	MS.gene88681	PPI
MS.gene88681	MS.gene049705	PPI
MS.gene88680	MS.gene88681	PPI
MS.gene88681	MS.gene070907	PPI
MS.gene070241	MS.gene88681	PPI
MS.gene88681	MS.gene04392	PPI
MS.gene88681	MS.gene010219	PPI
MS.gene88681	MS.gene05608	PPI
MS.gene88681	MS.gene040383	PPI
MS.gene88681	MS.gene68545	PPI
MS.gene061876	MS.gene88681	PPI
MS.gene88681	MS.gene070242	PPI
MS.gene012391	MS.gene88681	PPI
MS.gene88681	MS.gene82546	PPI
MS.gene070244	MS.gene88681	PPI
MS.gene88681	MS.gene048746	PPI
MS.gene060463	MS.gene88681	PPI
MS.gene88681	MS.gene08122	PPI
MS.gene88681	MS.gene09873	PPI
MS.gene88681	MS.gene022932	PPI
MS.gene31196	MS.gene88681	PPI
MS.gene88681	MS.gene043967	PPI
MS.gene88681	MS.gene62520	PPI
MS.gene88681	MS.gene08053	PPI
MS.gene88681	MS.gene035897	PPI
MS.gene88681	MS.gene035721	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88681.t1	MTR_7g029510	100.000	103	0	10	112	1	103	2.21e-68	200
MS.gene88681.t1	MTR_7g099610	100.000	103	0	10	112	1	103	2.21e-68	200
MS.gene88681.t1	MTR_5g063620	100.000	103	0	10	112	1	103	2.21e-68	200
MS.gene88681.t1	MTR_4g061827	100.000	103	0	10	112	1	103	2.21e-68	200
MS.gene88681.t1	MTR_4g061920	100.000	103	0	10	112	1	103	2.21e-68	200
MS.gene88681.t1	MTR_3g462400	100.000	103	0	10	112	1	103	2.21e-68	200
MS.gene88681.t1	MTR_3g054380	100.000	103	0	10	112	1	103	2.21e-68	200
MS.gene88681.t1	MTR_3g462730	100.000	103	0	10	112	1	103	2.21e-68	200
MS.gene88681.t1	MTR_3g462510	100.000	103	0	10	112	1	103	2.21e-68	200
MS.gene88681.t1	MTR_3g070920	100.000	103	0	10	112	1	103	2.21e-68	200
MS.gene88681.t1	MTR_3g061440	100.000	103	0	10	112	1	103	2.21e-68	200
MS.gene88681.t1	MTR_3g462410	100.000	103	0	10	112	1	103	2.21e-68	200
MS.gene88681.t1	MTR_2g096100	100.000	103	0	10	112	1	103	2.21e-68	200
MS.gene88681.t1	MTR_8g038460	100.000	103	0	10	112	1	103	2.21e-68	200
MS.gene88681.t1	MTR_4g128150	100.000	102	0	10	111	1	102	1.73e-66	205
MS.gene88681.t1	MTR_7g029520	84.000	50	8	36	85	1	50	3.77e-21	86.7

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88681.t1	AT5G59970	100.000	103	0	10	112	1	103	2.31e-68	200
MS.gene88681.t1	AT5G59690	100.000	103	0	10	112	1	103	2.31e-68	200
MS.gene88681.t1	AT5G59970	100.000	103	0	10	112	1	103	2.31e-68	200
MS.gene88681.t1	AT3G46320	100.000	103	0	10	112	1	103	2.31e-68	200
MS.gene88681.t1	AT3G45930	100.000	103	0	10	112	1	103	2.31e-68	200
MS.gene88681.t1	AT3G53730	100.000	103	0	10	112	1	103	2.31e-68	200
MS.gene88681.t1	AT2G28740	100.000	103	0	10	112	1	103	2.31e-68	200
MS.gene88681.t1	AT1G07660	100.000	103	0	10	112	1	103	2.31e-68	200
MS.gene88681.t1	AT1G07820	100.000	103	0	10	112	1	103	2.31e-68	200
MS.gene88681.t1	AT1G07820	100.000	103	0	10	112	1	103	2.31e-68	200
MS.gene88681.t1	AT1G07660	83.495	103	1	10	112	1	86	3.60e-51	156

Find 27 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTCTTCGTGATAACATTC+AGG	0.304269	8.4:-6210352	MS.gene88681:CDS
GGCAAAGGAGGAAAGGGTTT+AGG	0.384869	8.4:-6210402	MS.gene88681:CDS
CTTGCGAGACGACGAATTGC+AGG	0.403001	8.4:+6210318	None:intergenic
GGAGGAAAGGGTTTAGGAAA+GGG	0.429069	8.4:-6210396	MS.gene88681:CDS
GGAAGGACTCTTTACGGATT+CGG	0.436444	8.4:-6210132	MS.gene88681:CDS
AGGCAGGGAAGGACTCTTTA+CGG	0.443617	8.4:-6210138	MS.gene88681:CDS
TGTTTACGCTCTCAAGAGGC+AGG	0.493747	8.4:-6210154	MS.gene88681:CDS
GTTCTTCGTGATAACATTCA+GGG	0.504718	8.4:-6210351	MS.gene88681:CDS
AGGAAGAGGCAAAGGAGGAA+AGG	0.518412	8.4:-6210409	MS.gene88681:CDS
GGAAGAGGCAAAGGAGGAAA+GGG	0.527098	8.4:-6210408	MS.gene88681:CDS
AGGAGGAAAGGGTTTAGGAA+AGG	0.528235	8.4:-6210397	MS.gene88681:CDS
GGAAAGGGTTTAGGAAAGGG+AGG	0.536831	8.4:-6210393	MS.gene88681:CDS
CCGTAAAACTGTTACTGCGA+TGG	0.549023	8.4:-6210181	MS.gene88681:CDS
ACGGCGAGCATGTTCAGTGT+AGG	0.552626	8.4:+6210200	None:intergenic
GCGATGTCAGGAAGAGGCAA+AGG	0.557914	8.4:-6210417	MS.gene88681:CDS
TGTTATCACGAAGAACCTTA+CGG	0.570202	8.4:+6210358	None:intergenic
TTCACAGCGATGTCAGGAAG+AGG	0.573275	8.4:-6210423	MS.gene88681:CDS
AGGAGCAAAACGACACCGTA+AGG	0.575994	8.4:-6210373	MS.gene88681:CDS
ACGCTCTCAAGAGGCAGGGA+AGG	0.583359	8.4:-6210149	MS.gene88681:CDS
ATGTCAGGAAGAGGCAAAGG+AGG	0.592835	8.4:-6210414	MS.gene88681:CDS
AGGACTCTTTACGGATTCGG+TGG	0.600535	8.4:-6210129	MS.gene88681:CDS
ATGTTGTTTACGCTCTCAAG+AGG	0.616421	8.4:-6210158	MS.gene88681:CDS
GTTTACGCTCTCAAGAGGCA+GGG	0.624823	8.4:-6210153	MS.gene88681:CDS
TTAATCTATGAAGAAACTCG+TGG	0.634992	8.4:-6210267	MS.gene88681:CDS
ATTCGTCGTCTCGCAAGACG+TGG	0.635108	8.4:-6210312	MS.gene88681:CDS
CGTCGTCTCGCAAGACGTGG+TGG	0.642630	8.4:-6210309	MS.gene88681:CDS
GGTGGTGTGAAGCGTATCAG+TGG	0.669421	8.4:-6210291	MS.gene88681:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTAATCTATGAAGAAACTCG+TGG	-	chr8.4:6210299-6210318	MS.gene88681:CDS	30.0%
	AAAAATTTCACAGCGATGTC+AGG	-	chr8.4:6210137-6210156	MS.gene88681:CDS	35.0%
	GTTCTTCGTGATAACATTCA+GGG	-	chr8.4:6210215-6210234	MS.gene88681:CDS	35.0%
	TGTTATCACGAAGAACCTTA+CGG	+	chr8.4:6210211-6210230	None:intergenic	35.0%
	GGTTCTTCGTGATAACATTC+AGG	-	chr8.4:6210214-6210233	MS.gene88681:CDS	40.0%
!	ATGTTGTTTACGCTCTCAAG+AGG	-	chr8.4:6210408-6210427	MS.gene88681:CDS	40.0%
!	CCATCGCAGTAACAGTTTTA+CGG	+	chr8.4:6210388-6210407	None:intergenic	40.0%
	AGGAGGAAAGGGTTTAGGAA+AGG	-	chr8.4:6210169-6210188	MS.gene88681:CDS	45.0%
	CCGTAAAACTGTTACTGCGA+TGG	-	chr8.4:6210385-6210404	MS.gene88681:CDS	45.0%
	GGAAGGACTCTTTACGGATT+CGG	-	chr8.4:6210434-6210453	MS.gene88681:CDS	45.0%
	GGAGGAAAGGGTTTAGGAAA+GGG	-	chr8.4:6210170-6210189	MS.gene88681:CDS	45.0%
	AGGAAGAGGCAAAGGAGGAA+AGG	-	chr8.4:6210157-6210176	MS.gene88681:CDS	50.0%
	AGGACTCTTTACGGATTCGG+TGG	-	chr8.4:6210437-6210456	MS.gene88681:CDS	50.0%
	AGGAGCAAAACGACACCGTA+AGG	-	chr8.4:6210193-6210212	MS.gene88681:CDS	50.0%
	AGGCAGGGAAGGACTCTTTA+CGG	-	chr8.4:6210428-6210447	MS.gene88681:CDS	50.0%
	ATGTCAGGAAGAGGCAAAGG+AGG	-	chr8.4:6210152-6210171	MS.gene88681:CDS	50.0%
	GGAAAGGGTTTAGGAAAGGG+AGG	-	chr8.4:6210173-6210192	MS.gene88681:CDS	50.0%
	GGAAGAGGCAAAGGAGGAAA+GGG	-	chr8.4:6210158-6210177	MS.gene88681:CDS	50.0%
	GGCAAAGGAGGAAAGGGTTT+AGG	-	chr8.4:6210164-6210183	MS.gene88681:CDS	50.0%
	GTTTACGCTCTCAAGAGGCA+GGG	-	chr8.4:6210413-6210432	MS.gene88681:CDS	50.0%
	TGTTTACGCTCTCAAGAGGC+AGG	-	chr8.4:6210412-6210431	MS.gene88681:CDS	50.0%
	TTCACAGCGATGTCAGGAAG+AGG	-	chr8.4:6210143-6210162	MS.gene88681:CDS	50.0%
	ACGGCGAGCATGTTCAGTGT+AGG	+	chr8.4:6210369-6210388	None:intergenic	55.0%
	ATTCGTCGTCTCGCAAGACG+TGG	-	chr8.4:6210254-6210273	MS.gene88681:CDS	55.0%
	CTTGCGAGACGACGAATTGC+AGG	+	chr8.4:6210251-6210270	None:intergenic	55.0%
	GCGATGTCAGGAAGAGGCAA+AGG	-	chr8.4:6210149-6210168	MS.gene88681:CDS	55.0%
	GGTGGTGTGAAGCGTATCAG+TGG	-	chr8.4:6210275-6210294	MS.gene88681:CDS	55.0%
	ACGCTCTCAAGAGGCAGGGA+AGG	-	chr8.4:6210417-6210436	MS.gene88681:CDS	60.0%
	CGTCGTCTCGCAAGACGTGG+TGG	-	chr8.4:6210257-6210276	MS.gene88681:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	6210125	6210463	6210125	ID=MS.gene88681
chr8.4	mRNA	6210125	6210463	6210125	ID=MS.gene88681.t1;Parent=MS.gene88681
chr8.4	exon	6210125	6210463	6210125	ID=MS.gene88681.t1.exon1;Parent=MS.gene88681.t1
chr8.4	CDS	6210125	6210463	6210125	ID=cds.MS.gene88681.t1;Parent=MS.gene88681.t1

Gene Sequence	>MS.gene88681 AGAGAGAAACTCAAAAATTTCACAGCGATGTCAGGAAGAGGCAAAGGAGGAAAGGGTTTAGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTTCGTGATAACATTCAGGGAATTACAAAGCCTGCAATTCGTCGTCTCGCAAGACGTGGTGGTGTGAAGCGTATCAGTGGCTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAATGTGATTCGTGATGCTGTCACCTACACTGAACATGCTCGCCGTAAAACTGTTACTGCGATGGATGTTGTTTACGCTCTCAAGAGGCAGGGAAGGACTCTTTACGGATTCGGTGGTTAG
Protein sequence	>MS.gene88681.t1 REKLKNFTAMSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG