AlfalfaGEDB | MS.gene008276

M. sativa cultivar XinJiangDaYe / MS.gene008276

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008276.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008276.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008276.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene04576	MS.gene008276	PPI
MS.gene008276	MS.gene05608	PPI
MS.gene89487	MS.gene008276	PPI
MS.gene035719	MS.gene008276	PPI
MS.gene58854	MS.gene008276	PPI
MS.gene70955	MS.gene008276	PPI
MS.gene04392	MS.gene008276	PPI
MS.gene072493	MS.gene008276	PPI
MS.gene035720	MS.gene008276	PPI
MS.gene30920	MS.gene008276	PPI
MS.gene048746	MS.gene008276	PPI
MS.gene010220	MS.gene008276	PPI
MS.gene08053	MS.gene008276	PPI
MS.gene061872	MS.gene008276	PPI
MS.gene008276	MS.gene60093	PPI
MS.gene008276	MS.gene09873	PPI
MS.gene88680	MS.gene008276	PPI
MS.gene070241	MS.gene008276	PPI
MS.gene049821	MS.gene008276	PPI
MS.gene022946	MS.gene008276	PPI
MS.gene62520	MS.gene008276	PPI
MS.gene04064	MS.gene008276	PPI
MS.gene008276	MS.gene04972	PPI
MS.gene061876	MS.gene008276	PPI
MS.gene008276	MS.gene62520	PPI
MS.gene05608	MS.gene008276	PPI
MS.gene008276	MS.gene018578	PPI
MS.gene012391	MS.gene008276	PPI
MS.gene070244	MS.gene008276	PPI
MS.gene09871	MS.gene008276	PPI
MS.gene035897	MS.gene008276	PPI
MS.gene64920	MS.gene008276	PPI
MS.gene008276	MS.gene018580	PPI
MS.gene008276	MS.gene004124	PPI
MS.gene060463	MS.gene008276	PPI
MS.gene31196	MS.gene008276	PPI
MS.gene008276	MS.gene04052	PPI
MS.gene008276	MS.gene84536	PPI
MS.gene010219	MS.gene008276	PPI
MS.gene008276	MS.gene061866	PPI
MS.gene060465	MS.gene008276	PPI
MS.gene008276	MS.gene64650	PPI
MS.gene008276	MS.gene048705	PPI
MS.gene008276	MS.gene061874	PPI
MS.gene04068	MS.gene008276	PPI
MS.gene008276	MS.gene061869	PPI
MS.gene04434	MS.gene008276	PPI
MS.gene08121	MS.gene008276	PPI
MS.gene008276	MS.gene004121	PPI
MS.gene022932	MS.gene008276	PPI
MS.gene88681	MS.gene008276	PPI
MS.gene008276	MS.gene061867	PPI
MS.gene035718	MS.gene008276	PPI
MS.gene035896	MS.gene008276	PPI
MS.gene73136	MS.gene008276	PPI
MS.gene070907	MS.gene008276	PPI
MS.gene008276	MS.gene05619	PPI
MS.gene008276	MS.gene73136	PPI
MS.gene009027	MS.gene008276	PPI
MS.gene09873	MS.gene008276	PPI
MS.gene78578	MS.gene008276	PPI
MS.gene008276	MS.gene89488	PPI
MS.gene04052	MS.gene008276	PPI
MS.gene08122	MS.gene008276	PPI
MS.gene048705	MS.gene008276	PPI
MS.gene05623	MS.gene008276	PPI
MS.gene043967	MS.gene008276	PPI
MS.gene008276	MS.gene61017	PPI
MS.gene008276	MS.gene04576	PPI
MS.gene84536	MS.gene008276	PPI
MS.gene028629	MS.gene008276	PPI
MS.gene008276	MS.gene012390	PPI
MS.gene82546	MS.gene008276	PPI
MS.gene04433	MS.gene008276	PPI
MS.gene04067	MS.gene008276	PPI
MS.gene71215	MS.gene008276	PPI
MS.gene061866	MS.gene008276	PPI
MS.gene061870	MS.gene008276	PPI
MS.gene008276	MS.gene010219	PPI
MS.gene008276	MS.gene68545	PPI
MS.gene008276	MS.gene016624	PPI
MS.gene66225	MS.gene008276	PPI
MS.gene071136	MS.gene008276	PPI
MS.gene040383	MS.gene008276	PPI
MS.gene070242	MS.gene008276	PPI
MS.gene012390	MS.gene008276	PPI
MS.gene79195	MS.gene008276	PPI
MS.gene035721	MS.gene008276	PPI
MS.gene056215	MS.gene008276	PPI
MS.gene064557	MS.gene008276	PPI
MS.gene008276	MS.gene035720	PPI
MS.gene038469	MS.gene008276	PPI
MS.gene61017	MS.gene008276	PPI
MS.gene04452	MS.gene008276	PPI
MS.gene008276	MS.gene049705	PPI
MS.gene035787	MS.gene008276	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008276.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene008276.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene008276.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene008276.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene008276.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene008276.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene008276.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene008276.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene008276.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene008276.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene008276.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene008276.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene008276.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene008276.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene008276.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene008276.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008276.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene008276.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene008276.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene008276.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene008276.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene008276.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene008276.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene008276.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene008276.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene008276.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene008276.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 36 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCGCCAATCGACGAATTGC+AGG	0.267346	3.1:+54915479	None:intergenic
GGAAGAACCCTCTATGGATT+TGG	0.370047	3.1:-54915293	MS.gene008276:CDS
AGACAAGGAAGAACCCTCTA+TGG	0.396310	3.1:-54915299	MS.gene008276:CDS
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	3.1:-54915408	MS.gene008276:CDS
GGGAGGTAAGGGACTTGGAA+AGG	0.449159	3.1:-54915558	MS.gene008276:CDS
GGAGGTAAGGGACTTGGAAA+GGG	0.467307	3.1:-54915557	MS.gene008276:CDS
GGAAAGGGAGGTAAGGGACT+TGG	0.470633	3.1:-54915563	MS.gene008276:CDS
TTCAACCTCCAAATCCATAG+AGG	0.476286	3.1:+54915285	None:intergenic
CTGTTGAAAATGTCAGGTCG+TGG	0.487154	3.1:-54915584	None:intergenic
CAATCGACGAATTGCAGGCT+TGG	0.491738	3.1:+54915484	None:intergenic
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.1:-54915570	MS.gene008276:CDS
AGTTCTCCGCGATAACATCC+AGG	0.504812	3.1:-54915513	MS.gene008276:CDS
AGAACCCTCTATGGATTTGG+AGG	0.508817	3.1:-54915290	MS.gene008276:CDS
GAAGATCTTCAAGACACCTC+TGG	0.510902	3.1:+54915412	None:intergenic
CAAGCCTGCAATTCGTCGAT+TGG	0.511374	3.1:-54915483	MS.gene008276:CDS
TTACATACACTGAGCATGCT+AGG	0.520723	3.1:-54915364	MS.gene008276:CDS
GAGGAAGACGGTGACAGCCA+TGG	0.546349	3.1:-54915342	MS.gene008276:CDS
AGGGAGGTGCAAAGAGGCAT+AGG	0.547891	3.1:-54915538	MS.gene008276:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.548959	3.1:-54915578	MS.gene008276:CDS
GGCGGAGTTAAGAGAATCAG+TGG	0.555739	3.1:-54915452	MS.gene008276:CDS
GGTAAGGGACTTGGAAAGGG+AGG	0.560743	3.1:-54915554	MS.gene008276:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	3.1:-54915569	MS.gene008276:CDS
GTCTATGCTTTGAAGAGACA+AGG	0.570048	3.1:-54915314	MS.gene008276:CDS
CGTCGATTGGCGAGAAGAGG+CGG	0.584174	3.1:-54915470	MS.gene008276:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.1:-54915579	None:intergenic
ATTCGTCGATTGGCGAGAAG+AGG	0.604517	3.1:-54915473	MS.gene008276:CDS
TTGCAGGCTTGGTGATACCC+TGG	0.613296	3.1:+54915495	None:intergenic
CAAAGCATAGACAACATCCA+TGG	0.629842	3.1:+54915325	None:intergenic
TCAACCTCCAAATCCATAGA+GGG	0.633476	3.1:+54915286	None:intergenic
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.1:-54915575	MS.gene008276:CDS
TGATACCCTGGATGTTATCG+CGG	0.665173	3.1:+54915507	None:intergenic
TGAGCATGCTAGGAGGAAGA+CGG	0.671956	3.1:-54915354	MS.gene008276:CDS
GTTCTCCGCGATAACATCCA+GGG	0.672360	3.1:-54915512	MS.gene008276:CDS
TTGGAAAGGGAGGTGCAAAG+AGG	0.683014	3.1:-54915544	MS.gene008276:CDS
TTGATCTATGAAGAAACCAG+AGG	0.704889	3.1:-54915428	MS.gene008276:CDS
CATACACTGAGCATGCTAGG+AGG	0.707859	3.1:-54915361	MS.gene008276:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATCTATGAAGAAACCAG+AGG	-	chr3.1:54915433-54915452	MS.gene008276:CDS	35.0%
	AGGTGTCTTGAAGATCTTCT+TGG	-	chr3.1:54915453-54915472	MS.gene008276:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	+	chr3.1:54915539-54915558	None:intergenic	40.0%
	TCAACCTCCAAATCCATAGA+GGG	+	chr3.1:54915578-54915597	None:intergenic	40.0%
	TTACATACACTGAGCATGCT+AGG	-	chr3.1:54915497-54915516	MS.gene008276:CDS	40.0%
!!	GTCTATGCTTTGAAGAGACA+AGG	-	chr3.1:54915547-54915566	MS.gene008276:CDS	40.0%
	AGAACCCTCTATGGATTTGG+AGG	-	chr3.1:54915571-54915590	MS.gene008276:CDS	45.0%
	AGACAAGGAAGAACCCTCTA+TGG	-	chr3.1:54915562-54915581	MS.gene008276:CDS	45.0%
	GAAGATCTTCAAGACACCTC+TGG	+	chr3.1:54915452-54915471	None:intergenic	45.0%
	GGAAGAACCCTCTATGGATT+TGG	-	chr3.1:54915568-54915587	MS.gene008276:CDS	45.0%
	TGATACCCTGGATGTTATCG+CGG	+	chr3.1:54915357-54915376	None:intergenic	45.0%
	AGTTCTCCGCGATAACATCC+AGG	-	chr3.1:54915348-54915367	MS.gene008276:CDS	50.0%
	CAAGCCTGCAATTCGTCGAT+TGG	-	chr3.1:54915378-54915397	MS.gene008276:CDS	50.0%
	CAATCGACGAATTGCAGGCT+TGG	+	chr3.1:54915380-54915399	None:intergenic	50.0%
	CATACACTGAGCATGCTAGG+AGG	-	chr3.1:54915500-54915519	MS.gene008276:CDS	50.0%
	GGAGGTAAGGGACTTGGAAA+GGG	-	chr3.1:54915304-54915323	MS.gene008276:CDS	50.0%
	GGCGGAGTTAAGAGAATCAG+TGG	-	chr3.1:54915409-54915428	MS.gene008276:CDS	50.0%
	GTTCTCCGCGATAACATCCA+GGG	-	chr3.1:54915349-54915368	MS.gene008276:CDS	50.0%
	TGAGCATGCTAGGAGGAAGA+CGG	-	chr3.1:54915507-54915526	MS.gene008276:CDS	50.0%
!	ATTCGTCGATTGGCGAGAAG+AGG	-	chr3.1:54915388-54915407	MS.gene008276:CDS	50.0%
!	TTGGAAAGGGAGGTGCAAAG+AGG	-	chr3.1:54915317-54915336	MS.gene008276:CDS	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	-	chr3.1:54915291-54915310	MS.gene008276:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	-	chr3.1:54915286-54915305	MS.gene008276:CDS	55.0%
	CTCGCCAATCGACGAATTGC+AGG	+	chr3.1:54915385-54915404	None:intergenic	55.0%
	GGAAAGGGAGGTAAGGGACT+TGG	-	chr3.1:54915298-54915317	MS.gene008276:CDS	55.0%
	GGGAGGTAAGGGACTTGGAA+AGG	-	chr3.1:54915303-54915322	MS.gene008276:CDS	55.0%
	GGTAAGGGACTTGGAAAGGG+AGG	-	chr3.1:54915307-54915326	MS.gene008276:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	-	chr3.1:54915292-54915311	MS.gene008276:CDS	55.0%
!	AGGGAGGTGCAAAGAGGCAT+AGG	-	chr3.1:54915323-54915342	MS.gene008276:CDS	55.0%
!!	TTGCAGGCTTGGTGATACCC+TGG	+	chr3.1:54915369-54915388	None:intergenic	55.0%
	GAGGAAGACGGTGACAGCCA+TGG	-	chr3.1:54915519-54915538	MS.gene008276:CDS	60.0%
!	CGTCGATTGGCGAGAAGAGG+CGG	-	chr3.1:54915391-54915410	MS.gene008276:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	54915286	54915597	54915286	ID=MS.gene008276
chr3.1	mRNA	54915286	54915597	54915286	ID=MS.gene008276.t1;Parent=MS.gene008276
chr3.1	exon	54915286	54915597	54915286	ID=MS.gene008276.t1.exon1;Parent=MS.gene008276.t1
chr3.1	CDS	54915286	54915597	54915286	ID=cds.MS.gene008276.t1;Parent=MS.gene008276.t1

Gene Sequence	>MS.gene008276 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTTGGAAAGGGAGGTGCAAAGAGGCATAGGAAAGTTCTCCGCGATAACATCCAGGGTATCACCAAGCCTGCAATTCGTCGATTGGCGAGAAGAGGCGGAGTTAAGAGAATCAGTGGATTGATCTATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAACGTCATTCGTGATGCAGTTACATACACTGAGCATGCTAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAAGGAAGAACCCTCTATGGATTTGGAGGTTGA
Protein sequence	>MS.gene008276.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG