AlfalfaGEDB | MS.gene04064

M. sativa cultivar XinJiangDaYe / MS.gene04064

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04064.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04064.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04064.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene04064	MS.gene061866	PPI
MS.gene060463	MS.gene04064	PPI
MS.gene04064	MS.gene64650	PPI
MS.gene04064	MS.gene048705	PPI
MS.gene04064	MS.gene061874	PPI
MS.gene04064	MS.gene061869	PPI
MS.gene04392	MS.gene04064	PPI
MS.gene30920	MS.gene04064	PPI
MS.gene04068	MS.gene04064	PPI
MS.gene04064	MS.gene004121	PPI
MS.gene04434	MS.gene04064	PPI
MS.gene022946	MS.gene04064	PPI
MS.gene04064	MS.gene061867	PPI
MS.gene08121	MS.gene04064	PPI
MS.gene04064	MS.gene008276	PPI
MS.gene022932	MS.gene04064	PPI
MS.gene04064	MS.gene05619	PPI
MS.gene04064	MS.gene73136	PPI
MS.gene012391	MS.gene04064	PPI
MS.gene88681	MS.gene04064	PPI
MS.gene04064	MS.gene049821	PPI
MS.gene070244	MS.gene04064	PPI
MS.gene035897	MS.gene04064	PPI
MS.gene035896	MS.gene04064	PPI
MS.gene64920	MS.gene04064	PPI
MS.gene04064	MS.gene89488	PPI
MS.gene070907	MS.gene04064	PPI
MS.gene009027	MS.gene04064	PPI
MS.gene04064	MS.gene035720	PPI
MS.gene04064	MS.gene61017	PPI
MS.gene04064	MS.gene04576	PPI
MS.gene31196	MS.gene04064	PPI
MS.gene04064	MS.gene012390	PPI
MS.gene08122	MS.gene04064	PPI
MS.gene060465	MS.gene04064	PPI
MS.gene05623	MS.gene04064	PPI
MS.gene043967	MS.gene04064	PPI
MS.gene04064	MS.gene010219	PPI
MS.gene04064	MS.gene68545	PPI
MS.gene71215	MS.gene04064	PPI
MS.gene04064	MS.gene016624	PPI
MS.gene061870	MS.gene04064	PPI
MS.gene035718	MS.gene04064	PPI
MS.gene071136	MS.gene04064	PPI
MS.gene78578	MS.gene04064	PPI
MS.gene79195	MS.gene04064	PPI
MS.gene035721	MS.gene04064	PPI
MS.gene04064	MS.gene049705	PPI
MS.gene064557	MS.gene04064	PPI
MS.gene038469	MS.gene04064	PPI
MS.gene04064	MS.gene070907	PPI
MS.gene028629	MS.gene04064	PPI
MS.gene82546	MS.gene04064	PPI
MS.gene04433	MS.gene04064	PPI
MS.gene04064	MS.gene05608	PPI
MS.gene04452	MS.gene04064	PPI
MS.gene035787	MS.gene04064	PPI
MS.gene04067	MS.gene04064	PPI
MS.gene04064	MS.gene82546	PPI
MS.gene89487	MS.gene04064	PPI
MS.gene035719	MS.gene04064	PPI
MS.gene04064	MS.gene60093	PPI
MS.gene58854	MS.gene04064	PPI
MS.gene04064	MS.gene08122	PPI
MS.gene04064	MS.gene09873	PPI
MS.gene66225	MS.gene04064	PPI
MS.gene70955	MS.gene04064	PPI
MS.gene040383	MS.gene04064	PPI
MS.gene070242	MS.gene04064	PPI
MS.gene072493	MS.gene04064	PPI
MS.gene048746	MS.gene04064	PPI
MS.gene010220	MS.gene04064	PPI
MS.gene08053	MS.gene04064	PPI
MS.gene061872	MS.gene04064	PPI
MS.gene04064	MS.gene04972	PPI
MS.gene88680	MS.gene04064	PPI
MS.gene04064	MS.gene62520	PPI
MS.gene070241	MS.gene04064	PPI
MS.gene056215	MS.gene04064	PPI
MS.gene049821	MS.gene04064	PPI
MS.gene04064	MS.gene018578	PPI
MS.gene04064	MS.gene018580	PPI
MS.gene04064	MS.gene004124	PPI
MS.gene061876	MS.gene04064	PPI
MS.gene09871	MS.gene04064	PPI
MS.gene04064	MS.gene04052	PPI
MS.gene04064	MS.gene84536	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04064.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04064.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04064.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04064.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04064.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04064.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04064.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04064.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04064.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04064.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04064.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04064.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04064.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04064.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04064.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene04064.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04064.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04064.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04064.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04064.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04064.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04064.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04064.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04064.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04064.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04064.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04064.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 37 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	3.1:+46761128	MS.gene04064:CDS
CTCGCCAATCGGCGAATAGC+AGG	0.421570	3.1:-46761057	None:intergenic
AGACAGGGAAGAACGCTCTA+TGG	0.434419	3.1:+46761237	MS.gene04064:CDS
GGAAGAACGCTCTATGGTTT+CGG	0.438131	3.1:+46761243	MS.gene04064:CDS
CAATCGGCGAATAGCAGGTT+TGG	0.445374	3.1:-46761052	None:intergenic
TGTCTATGCTTTGAAGAGAC+AGG	0.458633	3.1:+46761221	MS.gene04064:CDS
GGTAAGGGACTCGGAAAGGG+AGG	0.488695	3.1:+46760982	MS.gene04064:CDS
AGGCCGTGGAAAGGGAGGTA+AGG	0.492819	3.1:+46760966	MS.gene04064:CDS
CACCACCTCTTCTCGCCAAT+CGG	0.499060	3.1:-46761068	None:intergenic
GGAGGTAAGGGACTCGGAAA+GGG	0.499726	3.1:+46760979	MS.gene04064:CDS
CTCTTGAAAATGTCAGGCCG+TGG	0.503870	3.1:+46760952	None:intergenic
GAAGATCTTCAAGACACCTC+TGG	0.510902	3.1:-46761124	None:intergenic
GTCCCTTACCTCCCTTTCCA+CGG	0.513750	3.1:-46760969	None:intergenic
AAAATGTCAGGCCGTGGAAA+GGG	0.515750	3.1:+46760958	None:intergenic
GGGAGGTAAGGGACTCGGAA+AGG	0.518226	3.1:+46760978	MS.gene04064:CDS
AGTTCTTCGTGATAACATCC+AGG	0.522451	3.1:+46761023	MS.gene04064:CDS
CGCCGATTGGCGAGAAGAGG+TGG	0.530277	3.1:+46761066	MS.gene04064:CDS
GGAAAGGGAGGTAAGGGACT+CGG	0.535187	3.1:+46760973	MS.gene04064:CDS
CAAACCTGCTATTCGCCGAT+TGG	0.536762	3.1:+46761053	MS.gene04064:CDS
AGGGAGGTGCAAAGAGGCAT+AGG	0.547891	3.1:+46760998	MS.gene04064:CDS
GAAAATGTCAGGCCGTGGAA+AGG	0.550606	3.1:+46760957	None:intergenic
GGCCGTGGAAAGGGAGGTAA+GGG	0.552535	3.1:+46760967	MS.gene04064:CDS
GAGGAAGACGGTGACAGCCA+TGG	0.561434	3.1:+46761194	MS.gene04064:CDS
GTCTATGCTTTGAAGAGACA+GGG	0.584038	3.1:+46761222	MS.gene04064:CDS
TGACATACACTGAGCACGCA+AGG	0.587222	3.1:+46761172	MS.gene04064:CDS
TAGCAGGTTTGGTGATACCC+TGG	0.596796	3.1:-46761041	None:intergenic
GGTGGTGTCAAGAGAATCAG+TGG	0.607763	3.1:+46761084	MS.gene04064:CDS
ATTCGCCGATTGGCGAGAAG+AGG	0.615284	3.1:+46761063	MS.gene04064:CDS
AGAACGCTCTATGGTTTCGG+AGG	0.628880	3.1:+46761246	MS.gene04064:CDS
CAAAGCATAGACAACATCCA+TGG	0.629842	3.1:-46761211	None:intergenic
ATGTCAGGCCGTGGAAAGGG+AGG	0.634999	3.1:+46760961	MS.gene04064:CDS
GTTCTTCGTGATAACATCCA+GGG	0.641996	3.1:+46761024	MS.gene04064:CDS
CAGTGTATGTCACAGCATCA+CGG	0.646580	3.1:-46761161	None:intergenic
TGAGCACGCAAGGAGGAAGA+CGG	0.652638	3.1:+46761182	MS.gene04064:CDS
TCGGAAAGGGAGGTGCAAAG+AGG	0.683014	3.1:+46760992	MS.gene04064:CDS
TTGATATATGAAGAAACCAG+AGG	0.683025	3.1:+46761108	MS.gene04064:CDS
CATACACTGAGCACGCAAGG+AGG	0.730442	3.1:+46761175	MS.gene04064:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATATATGAAGAAACCAG+AGG	+	chr3.1:46761108-46761127	MS.gene04064:CDS	30.0%
	AGGTGTCTTGAAGATCTTCT+TGG	+	chr3.1:46761128-46761147	MS.gene04064:CDS	40.0%
	AGTTCTTCGTGATAACATCC+AGG	+	chr3.1:46761023-46761042	MS.gene04064:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	-	chr3.1:46761214-46761233	None:intergenic	40.0%
	GTTCTTCGTGATAACATCCA+GGG	+	chr3.1:46761024-46761043	MS.gene04064:CDS	40.0%
!!	GTCTATGCTTTGAAGAGACA+GGG	+	chr3.1:46761222-46761241	MS.gene04064:CDS	40.0%
!!	TGTCTATGCTTTGAAGAGAC+AGG	+	chr3.1:46761221-46761240	MS.gene04064:CDS	40.0%
	CAGTGTATGTCACAGCATCA+CGG	-	chr3.1:46761164-46761183	None:intergenic	45.0%
	GAAGATCTTCAAGACACCTC+TGG	-	chr3.1:46761127-46761146	None:intergenic	45.0%
	GGAAGAACGCTCTATGGTTT+CGG	+	chr3.1:46761243-46761262	MS.gene04064:CDS	45.0%
	AGAACGCTCTATGGTTTCGG+AGG	+	chr3.1:46761246-46761265	MS.gene04064:CDS	50.0%
	AGACAGGGAAGAACGCTCTA+TGG	+	chr3.1:46761237-46761256	MS.gene04064:CDS	50.0%
	CAAACCTGCTATTCGCCGAT+TGG	+	chr3.1:46761053-46761072	MS.gene04064:CDS	50.0%
	CAATCGGCGAATAGCAGGTT+TGG	-	chr3.1:46761055-46761074	None:intergenic	50.0%
	TGACATACACTGAGCACGCA+AGG	+	chr3.1:46761172-46761191	MS.gene04064:CDS	50.0%
!	GGTGGTGTCAAGAGAATCAG+TGG	+	chr3.1:46761084-46761103	MS.gene04064:CDS	50.0%
!!	TAGCAGGTTTGGTGATACCC+TGG	-	chr3.1:46761044-46761063	None:intergenic	50.0%
	CACCACCTCTTCTCGCCAAT+CGG	-	chr3.1:46761071-46761090	None:intergenic	55.0%
	CATACACTGAGCACGCAAGG+AGG	+	chr3.1:46761175-46761194	MS.gene04064:CDS	55.0%
	GGAAAGGGAGGTAAGGGACT+CGG	+	chr3.1:46760973-46760992	MS.gene04064:CDS	55.0%
	GGAGGTAAGGGACTCGGAAA+GGG	+	chr3.1:46760979-46760998	MS.gene04064:CDS	55.0%
	GTCCCTTACCTCCCTTTCCA+CGG	-	chr3.1:46760972-46760991	None:intergenic	55.0%
	TGAGCACGCAAGGAGGAAGA+CGG	+	chr3.1:46761182-46761201	MS.gene04064:CDS	55.0%
!	AGGGAGGTGCAAAGAGGCAT+AGG	+	chr3.1:46760998-46761017	MS.gene04064:CDS	55.0%
!	ATTCGCCGATTGGCGAGAAG+AGG	+	chr3.1:46761063-46761082	MS.gene04064:CDS	55.0%
!	TCGGAAAGGGAGGTGCAAAG+AGG	+	chr3.1:46760992-46761011	MS.gene04064:CDS	55.0%
	AGGCCGTGGAAAGGGAGGTA+AGG	+	chr3.1:46760966-46760985	MS.gene04064:CDS	60.0%
	ATGTCAGGCCGTGGAAAGGG+AGG	+	chr3.1:46760961-46760980	MS.gene04064:CDS	60.0%
	CGTCTTCCTCCTTGCGTGCT+CGG	-	chr3.1:46761184-46761203	None:intergenic	60.0%
	CTCGCCAATCGGCGAATAGC+AGG	-	chr3.1:46761060-46761079	None:intergenic	60.0%
	GAGGAAGACGGTGACAGCCA+TGG	+	chr3.1:46761194-46761213	MS.gene04064:CDS	60.0%
	GGCCGTGGAAAGGGAGGTAA+GGG	+	chr3.1:46760967-46760986	MS.gene04064:CDS	60.0%
	GGGAGGTAAGGGACTCGGAA+AGG	+	chr3.1:46760978-46760997	MS.gene04064:CDS	60.0%
	GGTAAGGGACTCGGAAAGGG+AGG	+	chr3.1:46760982-46761001	MS.gene04064:CDS	60.0%
!	CGCCGATTGGCGAGAAGAGG+TGG	+	chr3.1:46761066-46761085	MS.gene04064:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	46760961	46761272	46760961	ID=MS.gene04064
chr3.1	mRNA	46760961	46761272	46760961	ID=MS.gene04064.t1;Parent=MS.gene04064
chr3.1	exon	46760961	46761272	46760961	ID=MS.gene04064.t1.exon1;Parent=MS.gene04064.t1
chr3.1	CDS	46760961	46761272	46760961	ID=cds.MS.gene04064.t1;Parent=MS.gene04064.t1

Gene Sequence	>MS.gene04064 ATGTCAGGCCGTGGAAAGGGAGGTAAGGGACTCGGAAAGGGAGGTGCAAAGAGGCATAGGAAAGTTCTTCGTGATAACATCCAGGGTATCACCAAACCTGCTATTCGCCGATTGGCGAGAAGAGGTGGTGTCAAGAGAATCAGTGGATTGATATATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAATGTCATCCGTGATGCTGTGACATACACTGAGCACGCAAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAGGGAAGAACGCTCTATGGTTTCGGAGGTTGA
Protein sequence	>MS.gene04064.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG