AlfalfaGEDB | MS.gene73136

M. sativa cultivar XinJiangDaYe / MS.gene73136

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73136.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73136.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73136.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049821	MS.gene73136	0.814642	1.39E-51	-1.69E-46
MS.gene050901	MS.gene73136	0.834591	2.74E-56	-1.69E-46
MS.gene051720	MS.gene73136	0.81145	6.96E-51	-1.69E-46
MS.gene05608	MS.gene73136	0.807222	5.62E-50	-1.69E-46
MS.gene056215	MS.gene73136	0.850035	2.20E-60	-1.69E-46
MS.gene061100	MS.gene73136	0.803418	3.52E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene035787	MS.gene73136	PPI
MS.gene89487	MS.gene73136	PPI
MS.gene73136	MS.gene84536	PPI
MS.gene035719	MS.gene73136	PPI
MS.gene58854	MS.gene73136	PPI
MS.gene70955	MS.gene73136	PPI
MS.gene040383	MS.gene73136	PPI
MS.gene73136	MS.gene60093	PPI
MS.gene070242	MS.gene73136	PPI
MS.gene072493	MS.gene73136	PPI
MS.gene035720	MS.gene73136	PPI
MS.gene30920	MS.gene73136	PPI
MS.gene048746	MS.gene73136	PPI
MS.gene010220	MS.gene73136	PPI
MS.gene08053	MS.gene73136	PPI
MS.gene061872	MS.gene73136	PPI
MS.gene88680	MS.gene73136	PPI
MS.gene070241	MS.gene73136	PPI
MS.gene056215	MS.gene73136	PPI
MS.gene049821	MS.gene73136	PPI
MS.gene73136	MS.gene04972	PPI
MS.gene022946	MS.gene73136	PPI
MS.gene62520	MS.gene73136	PPI
MS.gene73136	MS.gene004121	PPI
MS.gene73136	MS.gene62520	PPI
MS.gene04064	MS.gene73136	PPI
MS.gene73136	MS.gene018578	PPI
MS.gene061866	MS.gene73136	PPI
MS.gene061876	MS.gene73136	PPI
MS.gene05608	MS.gene73136	PPI
MS.gene73136	MS.gene018580	PPI
MS.gene012391	MS.gene73136	PPI
MS.gene73136	MS.gene004124	PPI
MS.gene09871	MS.gene73136	PPI
MS.gene035897	MS.gene73136	PPI
MS.gene04576	MS.gene73136	PPI
MS.gene060463	MS.gene73136	PPI
MS.gene73136	MS.gene04052	PPI
MS.gene73136	MS.gene061866	PPI
MS.gene73136	MS.gene64650	PPI
MS.gene73136	MS.gene048705	PPI
MS.gene04392	MS.gene73136	PPI
MS.gene73136	MS.gene061874	PPI
MS.gene73136	MS.gene04576	PPI
MS.gene73136	MS.gene061869	PPI
MS.gene04068	MS.gene73136	PPI
MS.gene04434	MS.gene73136	PPI
MS.gene08121	MS.gene73136	PPI
MS.gene73136	MS.gene061867	PPI
MS.gene022932	MS.gene73136	PPI
MS.gene73136	MS.gene008276	PPI
MS.gene88681	MS.gene73136	PPI
MS.gene070244	MS.gene73136	PPI
MS.gene035896	MS.gene73136	PPI
MS.gene73136	MS.gene05619	PPI
MS.gene64920	MS.gene73136	PPI
MS.gene008276	MS.gene73136	PPI
MS.gene070907	MS.gene73136	PPI
MS.gene009027	MS.gene73136	PPI
MS.gene31196	MS.gene73136	PPI
MS.gene010219	MS.gene73136	PPI
MS.gene73136	MS.gene035720	PPI
MS.gene04052	MS.gene73136	PPI
MS.gene73136	MS.gene61017	PPI
MS.gene08122	MS.gene73136	PPI
MS.gene060465	MS.gene73136	PPI
MS.gene05623	MS.gene73136	PPI
MS.gene043967	MS.gene73136	PPI
MS.gene73136	MS.gene012390	PPI
MS.gene84536	MS.gene73136	PPI
MS.gene028629	MS.gene73136	PPI
MS.gene73136	MS.gene049705	PPI
MS.gene73136	MS.gene010219	PPI
MS.gene73136	MS.gene05608	PPI
MS.gene04067	MS.gene73136	PPI
MS.gene71215	MS.gene73136	PPI
MS.gene73136	MS.gene89488	PPI
MS.gene73136	MS.gene68545	PPI
MS.gene061870	MS.gene73136	PPI
MS.gene66225	MS.gene73136	PPI
MS.gene73136	MS.gene016624	PPI
MS.gene035718	MS.gene73136	PPI
MS.gene071136	MS.gene73136	PPI
MS.gene012390	MS.gene73136	PPI
MS.gene09873	MS.gene73136	PPI
MS.gene73136	MS.gene09873	PPI
MS.gene78578	MS.gene73136	PPI
MS.gene79195	MS.gene73136	PPI
MS.gene035721	MS.gene73136	PPI
MS.gene048705	MS.gene73136	PPI
MS.gene064557	MS.gene73136	PPI
MS.gene038469	MS.gene73136	PPI
MS.gene82546	MS.gene73136	PPI
MS.gene61017	MS.gene73136	PPI
MS.gene04433	MS.gene73136	PPI
MS.gene04452	MS.gene73136	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73136.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene73136.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene73136.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene73136.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene73136.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene73136.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene73136.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene73136.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene73136.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene73136.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene73136.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene73136.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene73136.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene73136.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene73136.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene73136.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73136.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene73136.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene73136.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene73136.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene73136.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene73136.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene73136.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene73136.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene73136.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene73136.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene73136.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 33 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGCAAGGAAGAACGCTTTA+TGG	0.253984	3.4:-51070309	MS.gene73136:CDS
GGAAGAACGCTTTATGGATT+CGG	0.418585	3.4:-51070303	MS.gene73136:CDS
GGGAGGTAAGGGACTTGGAA+AGG	0.449159	3.4:-51070568	MS.gene73136:CDS
CTCTTGAAAATGTCAGGTCG+TGG	0.456977	3.4:-51070594	None:intergenic
GGAGGTAAGGGACTTGGAAA+GGG	0.467307	3.4:-51070567	MS.gene73136:CDS
GGAAAGGGAGGTAAGGGACT+TGG	0.470633	3.4:-51070573	MS.gene73136:CDS
CAAGCCAGCGATTCGTCGAT+TGG	0.479149	3.4:-51070493	MS.gene73136:CDS
CTCGCCAATCGACGAATCGC+TGG	0.484651	3.4:+51070489	None:intergenic
CAATCGACGAATCGCTGGCT+TGG	0.489647	3.4:+51070494	None:intergenic
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.4:-51070580	MS.gene73136:CDS
AGTTCTTCGTGACAACATCC+AGG	0.501637	3.4:-51070523	MS.gene73136:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.538992	3.4:-51070588	MS.gene73136:CDS
GAGGAAGACGGTTACAGCCA+TGG	0.550679	3.4:-51070352	MS.gene73136:CDS
GTTTATGCTTTGAAGAGGCA+AGG	0.559764	3.4:-51070324	MS.gene73136:CDS
GGTAAGGGACTTGGAAAGGG+AGG	0.560743	3.4:-51070564	MS.gene73136:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	3.4:-51070579	MS.gene73136:CDS
GGAAAATGTTATTCGTGATG+CGG	0.574605	3.4:-51070397	MS.gene73136:CDS
AGGGAGGTGCAAAGAGACAC+AGG	0.578665	3.4:-51070548	MS.gene73136:CDS
AATCAGCGGTTTGATCTATG+AGG	0.581523	3.4:-51070448	MS.gene73136:CDS
TCGCTGGCTTGGTGATACCC+TGG	0.584362	3.4:+51070505	None:intergenic
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.4:-51070589	None:intergenic
TTACTTACACTGAGCATGCA+AGG	0.587365	3.4:-51070374	MS.gene73136:CDS
CAAAGCATAAACAACATCCA+TGG	0.590717	3.4:+51070335	None:intergenic
CGTCGATTGGCGAGAAGAGG+CGG	0.591343	3.4:-51070480	MS.gene73136:CDS
ATTCGTCGATTGGCGAGAAG+AGG	0.593676	3.4:-51070483	MS.gene73136:CDS
AGAACGCTTTATGGATTCGG+AGG	0.604365	3.4:-51070300	MS.gene73136:CDS
GGCGGTGTTAAGAGAATCAG+CGG	0.618267	3.4:-51070462	MS.gene73136:CDS
TTGATCTATGAGGAAACAAG+AGG	0.641491	3.4:-51070438	MS.gene73136:CDS
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.4:-51070585	MS.gene73136:CDS
TGAGCATGCAAGGAGGAAGA+CGG	0.657376	3.4:-51070364	MS.gene73136:CDS
ATGTTGTTTATGCTTTGAAG+AGG	0.665215	3.4:-51070329	MS.gene73136:CDS
GTTCTTCGTGACAACATCCA+GGG	0.676380	3.4:-51070522	MS.gene73136:CDS
CTTACACTGAGCATGCAAGG+AGG	0.756108	3.4:-51070371	MS.gene73136:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGGAGTTCTTAAGATTTTCT+TGG	-	chr3.4:51070463-51070482	MS.gene73136:CDS	30.0%
!!	ATGTTGTTTATGCTTTGAAG+AGG	-	chr3.4:51070552-51070571	MS.gene73136:CDS	30.0%
	CAAAGCATAAACAACATCCA+TGG	+	chr3.4:51070549-51070568	None:intergenic	35.0%
	GGAAAATGTTATTCGTGATG+CGG	-	chr3.4:51070484-51070503	MS.gene73136:CDS	35.0%
	TTGATCTATGAGGAAACAAG+AGG	-	chr3.4:51070443-51070462	MS.gene73136:CDS	35.0%
	AATCAGCGGTTTGATCTATG+AGG	-	chr3.4:51070433-51070452	MS.gene73136:CDS	40.0%
	GGAAGAACGCTTTATGGATT+CGG	-	chr3.4:51070578-51070597	MS.gene73136:CDS	40.0%
	TTACTTACACTGAGCATGCA+AGG	-	chr3.4:51070507-51070526	MS.gene73136:CDS	40.0%
!!	GTTTATGCTTTGAAGAGGCA+AGG	-	chr3.4:51070557-51070576	MS.gene73136:CDS	40.0%
	AGAACGCTTTATGGATTCGG+AGG	-	chr3.4:51070581-51070600	MS.gene73136:CDS	45.0%
	AGGCAAGGAAGAACGCTTTA+TGG	-	chr3.4:51070572-51070591	MS.gene73136:CDS	45.0%
	AGTTCTTCGTGACAACATCC+AGG	-	chr3.4:51070358-51070377	MS.gene73136:CDS	45.0%
	GTTCTTCGTGACAACATCCA+GGG	-	chr3.4:51070359-51070378	MS.gene73136:CDS	45.0%
	CTTACACTGAGCATGCAAGG+AGG	-	chr3.4:51070510-51070529	MS.gene73136:CDS	50.0%
	GGAGGTAAGGGACTTGGAAA+GGG	-	chr3.4:51070314-51070333	MS.gene73136:CDS	50.0%
	TGAGCATGCAAGGAGGAAGA+CGG	-	chr3.4:51070517-51070536	MS.gene73136:CDS	50.0%
!	ATTCGTCGATTGGCGAGAAG+AGG	-	chr3.4:51070398-51070417	MS.gene73136:CDS	50.0%
!	GGCGGTGTTAAGAGAATCAG+CGG	-	chr3.4:51070419-51070438	MS.gene73136:CDS	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	-	chr3.4:51070301-51070320	MS.gene73136:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	-	chr3.4:51070296-51070315	MS.gene73136:CDS	55.0%
	CAAGCCAGCGATTCGTCGAT+TGG	-	chr3.4:51070388-51070407	MS.gene73136:CDS	55.0%
	GAGGAAGACGGTTACAGCCA+TGG	-	chr3.4:51070529-51070548	MS.gene73136:CDS	55.0%
	GGAAAGGGAGGTAAGGGACT+TGG	-	chr3.4:51070308-51070327	MS.gene73136:CDS	55.0%
	GGGAGGTAAGGGACTTGGAA+AGG	-	chr3.4:51070313-51070332	MS.gene73136:CDS	55.0%
	GGTAAGGGACTTGGAAAGGG+AGG	-	chr3.4:51070317-51070336	MS.gene73136:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	-	chr3.4:51070302-51070321	MS.gene73136:CDS	55.0%
!	AGGGAGGTGCAAAGAGACAC+AGG	-	chr3.4:51070333-51070352	MS.gene73136:CDS	55.0%
!	CAATCGACGAATCGCTGGCT+TGG	+	chr3.4:51070390-51070409	None:intergenic	55.0%
	CTCGCCAATCGACGAATCGC+TGG	+	chr3.4:51070395-51070414	None:intergenic	60.0%
!	CGTCGATTGGCGAGAAGAGG+CGG	-	chr3.4:51070401-51070420	MS.gene73136:CDS	60.0%
!!	TCGCTGGCTTGGTGATACCC+TGG	+	chr3.4:51070379-51070398	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	51070296	51070607	51070296	ID=MS.gene73136
chr3.4	mRNA	51070296	51070607	51070296	ID=MS.gene73136.t1;Parent=MS.gene73136
chr3.4	exon	51070296	51070607	51070296	ID=MS.gene73136.t1.exon1;Parent=MS.gene73136.t1
chr3.4	CDS	51070296	51070607	51070296	ID=cds.MS.gene73136.t1;Parent=MS.gene73136.t1

Gene Sequence	>MS.gene73136 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTTGGAAAGGGAGGTGCAAAGAGACACAGGAAAGTTCTTCGTGACAACATCCAGGGTATCACCAAGCCAGCGATTCGTCGATTGGCGAGAAGAGGCGGTGTTAAGAGAATCAGCGGTTTGATCTATGAGGAAACAAGAGGAGTTCTTAAGATTTTCTTGGAAAATGTTATTCGTGATGCGGTTACTTACACTGAGCATGCAAGGAGGAAGACGGTTACAGCCATGGATGTTGTTTATGCTTTGAAGAGGCAAGGAAGAACGCTTTATGGATTCGGAGGTTGA
Protein sequence	>MS.gene73136.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG