AlfalfaGEDB | MS.gene035719

M. sativa cultivar XinJiangDaYe / MS.gene035719

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035719.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035719.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035719.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene035719	MS.gene038469	PPI
MS.gene035719	MS.gene016624	PPI
MS.gene035719	MS.gene008276	PPI
MS.gene035787	MS.gene035719	PPI
MS.gene035719	MS.gene73136	PPI
MS.gene89487	MS.gene035719	PPI
MS.gene035719	MS.gene049821	PPI
MS.gene035719	MS.gene04068	PPI
MS.gene035719	MS.gene022946	PPI
MS.gene035719	MS.gene05623	PPI
MS.gene58854	MS.gene035719	PPI
MS.gene035719	MS.gene035720	PPI
MS.gene035719	MS.gene70955	PPI
MS.gene30920	MS.gene035719	PPI
MS.gene061872	MS.gene035719	PPI
MS.gene035719	MS.gene09871	PPI
MS.gene88680	MS.gene035719	PPI
MS.gene035719	MS.gene049705	PPI
MS.gene070241	MS.gene035719	PPI
MS.gene035719	MS.gene04434	PPI
MS.gene035719	MS.gene070907	PPI
MS.gene035719	MS.gene04392	PPI
MS.gene035719	MS.gene010219	PPI
MS.gene035719	MS.gene05608	PPI
MS.gene035719	MS.gene040383	PPI
MS.gene061876	MS.gene035719	PPI
MS.gene035719	MS.gene68545	PPI
MS.gene012391	MS.gene035719	PPI
MS.gene035719	MS.gene070242	PPI
MS.gene070244	MS.gene035719	PPI
MS.gene035719	MS.gene82546	PPI
MS.gene060463	MS.gene035719	PPI
MS.gene035719	MS.gene048746	PPI
MS.gene035719	MS.gene09873	PPI
MS.gene31196	MS.gene035719	PPI
MS.gene035719	MS.gene043967	PPI
MS.gene035719	MS.gene62520	PPI
MS.gene035719	MS.gene08053	PPI
MS.gene035719	MS.gene035897	PPI
MS.gene035719	MS.gene035721	PPI
MS.gene035719	MS.gene072493	PPI
MS.gene88681	MS.gene035719	PPI
MS.gene035719	MS.gene84536	PPI
MS.gene035719	MS.gene035896	PPI
MS.gene035718	MS.gene035719	PPI
MS.gene035719	MS.gene64650	PPI
MS.gene035719	MS.gene60093	PPI
MS.gene009027	MS.gene035719	PPI
MS.gene035719	MS.gene08122	PPI
MS.gene035719	MS.gene061869	PPI
MS.gene78578	MS.gene035719	PPI
MS.gene035719	MS.gene022932	PPI
MS.gene060465	MS.gene035719	PPI
MS.gene035719	MS.gene010220	PPI
MS.gene035719	MS.gene04972	PPI
MS.gene035719	MS.gene004121	PPI
MS.gene028629	MS.gene035719	PPI
MS.gene035719	MS.gene018578	PPI
MS.gene04433	MS.gene035719	PPI
MS.gene035719	MS.gene061867	PPI
MS.gene035719	MS.gene04064	PPI
MS.gene035719	MS.gene018580	PPI
MS.gene035719	MS.gene004124	PPI
MS.gene04067	MS.gene035719	PPI
MS.gene71215	MS.gene035719	PPI
MS.gene061870	MS.gene035719	PPI
MS.gene035719	MS.gene05619	PPI
MS.gene035719	MS.gene04052	PPI
MS.gene035719	MS.gene89488	PPI
MS.gene035719	MS.gene061866	PPI
MS.gene071136	MS.gene035719	PPI
MS.gene035719	MS.gene66225	PPI
MS.gene035719	MS.gene79195	PPI
MS.gene035719	MS.gene048705	PPI
MS.gene035719	MS.gene61017	PPI
MS.gene035719	MS.gene061874	PPI
MS.gene035719	MS.gene04576	PPI
MS.gene035719	MS.gene012390	PPI
MS.gene035719	MS.gene08121	PPI
MS.gene035719	MS.gene64920	PPI
MS.gene035719	MS.gene064557	PPI
MS.gene056215	MS.gene035719	PPI
MS.gene035719	MS.gene04452	PPI
MS.gene038469	MS.gene035719	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035719.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035719.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035719.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035719.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035719.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035719.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035719.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035719.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035719.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035719.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035719.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035719.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035719.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035719.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035719.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene035719.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035719.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035719.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035719.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035719.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035719.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035719.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035719.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035719.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035719.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035719.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035719.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 32 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGCAGGGAAGGACTCTTTA+TGG	0.247348	8.4:-6162521	MS.gene035719:CDS
GGAAGGACTCTTTATGGATT+TGG	0.297351	8.4:-6162515	MS.gene035719:CDS
TCCTTTGCCTCTTCCTGACA+TGG	0.418595	8.4:+6162799	None:intergenic
TCTTTATGGATTTGGCGGTT+AGG	0.439485	8.4:-6162507	None:intergenic
GGCAAAGGAGGAAAGGGTCT+TGG	0.446329	8.4:-6162785	MS.gene035719:CDS
CAAGAATTCACAGCCATGTC+AGG	0.481900	8.4:-6162812	None:intergenic
CGTTTACGCTCTCAAGAGGC+AGG	0.488613	8.4:-6162537	MS.gene035719:CDS
GGTTCTTCGCGATAACATCC+AGG	0.500175	8.4:-6162735	MS.gene035719:CDS
AGGACTCTTTATGGATTTGG+CGG	0.509079	8.4:-6162512	MS.gene035719:CDS
GGAGGAAAGGGTCTTGGAAA+GGG	0.511441	8.4:-6162779	MS.gene035719:CDS
TCGCAGGCTTCGTGATTCCC+TGG	0.511861	8.4:+6162717	None:intergenic
AGGAAGAGGCAAAGGAGGAA+AGG	0.518412	8.4:-6162792	MS.gene035719:CDS
GGAAGAGGCAAAGGAGGAAA+GGG	0.527098	8.4:-6162791	MS.gene035719:CDS
CCATCGCAGTAACAGTCTTA+CGG	0.545551	8.4:+6162564	None:intergenic
GCCATGTCAGGAAGAGGCAA+AGG	0.547501	8.4:-6162800	MS.gene035719:CDS
AGGAGGAAAGGGTCTTGGAA+AGG	0.553664	8.4:-6162780	MS.gene035719:CDS
CCGTAAGACTGTTACTGCGA+TGG	0.561095	8.4:-6162564	MS.gene035719:CDS
CTTGCGAGACGACGAATCGC+AGG	0.571080	8.4:+6162701	None:intergenic
TTCACAGCCATGTCAGGAAG+AGG	0.573281	8.4:-6162806	None:intergenic
AGGAGCAAAACGACACCGTA+AGG	0.575994	8.4:-6162756	MS.gene035719:CDS
TGTTATCGCGAAGAACCTTA+CGG	0.576355	8.4:+6162741	None:intergenic
ATGTCAGGAAGAGGCAAAGG+AGG	0.581057	8.4:-6162797	MS.gene035719:CDS
ACGCTCTCAAGAGGCAGGGA+AGG	0.583359	8.4:-6162532	MS.gene035719:CDS
ACGGCGAGCATGCTCTGTGT+AGG	0.590446	8.4:+6162583	None:intergenic
GGAAAGGGTCTTGGAAAGGG+AGG	0.621886	8.4:-6162776	MS.gene035719:CDS
ATTCGTCGTCTCGCAAGACG+TGG	0.624267	8.4:-6162695	MS.gene035719:CDS
TTAATCTATGAAGAAACTCG+TGG	0.634992	8.4:-6162650	MS.gene035719:CDS
ATGTCGTTTACGCTCTCAAG+AGG	0.636714	8.4:-6162541	MS.gene035719:CDS
CGTCGTCTCGCAAGACGTGG+TGG	0.642630	8.4:-6162692	MS.gene035719:CDS
GTTCTTCGCGATAACATCCA+GGG	0.657095	8.4:-6162734	MS.gene035719:CDS
GTTTACGCTCTCAAGAGGCA+GGG	0.663401	8.4:-6162536	MS.gene035719:CDS
GGTGGTGTGAAGCGTATCAG+TGG	0.669421	8.4:-6162674	MS.gene035719:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTAATCTATGAAGAAACTCG+TGG	-	chr8.4:6162655-6162674	MS.gene035719:CDS	30.0%
	AGGACTCTTTATGGATTTGG+CGG	-	chr8.4:6162793-6162812	MS.gene035719:CDS	40.0%
	GGAAGGACTCTTTATGGATT+TGG	-	chr8.4:6162790-6162809	MS.gene035719:CDS	40.0%
	TGTTATCGCGAAGAACCTTA+CGG	+	chr8.4:6162567-6162586	None:intergenic	40.0%
	CCATCGCAGTAACAGTCTTA+CGG	+	chr8.4:6162744-6162763	None:intergenic	45.0%
	GTTCTTCGCGATAACATCCA+GGG	-	chr8.4:6162571-6162590	MS.gene035719:CDS	45.0%
!	ATGTCGTTTACGCTCTCAAG+AGG	-	chr8.4:6162764-6162783	MS.gene035719:CDS	45.0%
	AGGAAGAGGCAAAGGAGGAA+AGG	-	chr8.4:6162513-6162532	MS.gene035719:CDS	50.0%
	AGGAGCAAAACGACACCGTA+AGG	-	chr8.4:6162549-6162568	MS.gene035719:CDS	50.0%
	AGGAGGAAAGGGTCTTGGAA+AGG	-	chr8.4:6162525-6162544	MS.gene035719:CDS	50.0%
	AGGCAGGGAAGGACTCTTTA+TGG	-	chr8.4:6162784-6162803	MS.gene035719:CDS	50.0%
	ATGTCAGGAAGAGGCAAAGG+AGG	-	chr8.4:6162508-6162527	MS.gene035719:CDS	50.0%
	CCGTAAGACTGTTACTGCGA+TGG	-	chr8.4:6162741-6162760	MS.gene035719:CDS	50.0%
	GGAAGAGGCAAAGGAGGAAA+GGG	-	chr8.4:6162514-6162533	MS.gene035719:CDS	50.0%
	GGAGGAAAGGGTCTTGGAAA+GGG	-	chr8.4:6162526-6162545	MS.gene035719:CDS	50.0%
	GGTTCTTCGCGATAACATCC+AGG	-	chr8.4:6162570-6162589	MS.gene035719:CDS	50.0%
	GTTTACGCTCTCAAGAGGCA+GGG	-	chr8.4:6162769-6162788	MS.gene035719:CDS	50.0%
	ATTCGTCGTCTCGCAAGACG+TGG	-	chr8.4:6162610-6162629	MS.gene035719:CDS	55.0%
	CGTTTACGCTCTCAAGAGGC+AGG	-	chr8.4:6162768-6162787	MS.gene035719:CDS	55.0%
	GGAAAGGGTCTTGGAAAGGG+AGG	-	chr8.4:6162529-6162548	MS.gene035719:CDS	55.0%
	GGCAAAGGAGGAAAGGGTCT+TGG	-	chr8.4:6162520-6162539	MS.gene035719:CDS	55.0%
	GGTGGTGTGAAGCGTATCAG+TGG	-	chr8.4:6162631-6162650	MS.gene035719:CDS	55.0%
	ACGCTCTCAAGAGGCAGGGA+AGG	-	chr8.4:6162773-6162792	MS.gene035719:CDS	60.0%
	ACGGCGAGCATGCTCTGTGT+AGG	+	chr8.4:6162725-6162744	None:intergenic	60.0%
	CTTGCGAGACGACGAATCGC+AGG	+	chr8.4:6162607-6162626	None:intergenic	60.0%
	TCGCAGGCTTCGTGATTCCC+TGG	+	chr8.4:6162591-6162610	None:intergenic	60.0%
	CGTCGTCTCGCAAGACGTGG+TGG	-	chr8.4:6162613-6162632	MS.gene035719:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	6162508	6162819	6162508	ID=MS.gene035719
chr8.4	mRNA	6162508	6162819	6162508	ID=MS.gene035719.t1;Parent=MS.gene035719
chr8.4	exon	6162508	6162819	6162508	ID=MS.gene035719.t1.exon1;Parent=MS.gene035719.t1
chr8.4	CDS	6162508	6162819	6162508	ID=cds.MS.gene035719.t1;Parent=MS.gene035719.t1

Gene Sequence	>MS.gene035719 ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTCTTGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTTCGCGATAACATCCAGGGAATCACGAAGCCTGCGATTCGTCGTCTCGCAAGACGTGGTGGTGTGAAGCGTATCAGTGGCTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAATGTGATTCGTGATGCTGTCACCTACACAGAGCATGCTCGCCGTAAGACTGTTACTGCGATGGATGTCGTTTACGCTCTCAAGAGGCAGGGAAGGACTCTTTATGGATTTGGCGGTTAG
Protein sequence	>MS.gene035719.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG