AlfalfaGEDB | MS.gene035720

M. sativa cultivar XinJiangDaYe / MS.gene035720

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035720.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035720.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035720.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene064557	MS.gene035720	PPI
MS.gene038469	MS.gene035720	PPI
MS.gene84536	MS.gene035720	PPI
MS.gene028629	MS.gene035720	PPI
MS.gene82546	MS.gene035720	PPI
MS.gene61017	MS.gene035720	PPI
MS.gene04433	MS.gene035720	PPI
MS.gene04452	MS.gene035720	PPI
MS.gene035720	MS.gene008276	PPI
MS.gene035787	MS.gene035720	PPI
MS.gene04067	MS.gene035720	PPI
MS.gene035720	MS.gene73136	PPI
MS.gene89487	MS.gene035720	PPI
MS.gene035719	MS.gene035720	PPI
MS.gene58854	MS.gene035720	PPI
MS.gene66225	MS.gene035720	PPI
MS.gene70955	MS.gene035720	PPI
MS.gene040383	MS.gene035720	PPI
MS.gene070242	MS.gene035720	PPI
MS.gene072493	MS.gene035720	PPI
MS.gene30920	MS.gene035720	PPI
MS.gene035720	MS.gene61017	PPI
MS.gene048746	MS.gene035720	PPI
MS.gene010220	MS.gene035720	PPI
MS.gene08053	MS.gene035720	PPI
MS.gene061872	MS.gene035720	PPI
MS.gene88680	MS.gene035720	PPI
MS.gene070241	MS.gene035720	PPI
MS.gene035720	MS.gene049705	PPI
MS.gene056215	MS.gene035720	PPI
MS.gene049821	MS.gene035720	PPI
MS.gene022946	MS.gene035720	PPI
MS.gene62520	MS.gene035720	PPI
MS.gene04064	MS.gene035720	PPI
MS.gene035720	MS.gene010219	PPI
MS.gene035720	MS.gene05608	PPI
MS.gene061876	MS.gene035720	PPI
MS.gene05608	MS.gene035720	PPI
MS.gene035720	MS.gene68545	PPI
MS.gene012391	MS.gene035720	PPI
MS.gene035720	MS.gene016624	PPI
MS.gene09871	MS.gene035720	PPI
MS.gene04576	MS.gene035720	PPI
MS.gene060463	MS.gene035720	PPI
MS.gene035720	MS.gene09873	PPI
MS.gene04392	MS.gene035720	PPI
MS.gene04068	MS.gene035720	PPI
MS.gene035720	MS.gene62520	PPI
MS.gene04434	MS.gene035720	PPI
MS.gene08121	MS.gene035720	PPI
MS.gene022932	MS.gene035720	PPI
MS.gene88681	MS.gene035720	PPI
MS.gene070244	MS.gene035720	PPI
MS.gene035897	MS.gene035720	PPI
MS.gene035720	MS.gene84536	PPI
MS.gene035896	MS.gene035720	PPI
MS.gene73136	MS.gene035720	PPI
MS.gene64920	MS.gene035720	PPI
MS.gene070907	MS.gene035720	PPI
MS.gene035720	MS.gene64650	PPI
MS.gene009027	MS.gene035720	PPI
MS.gene035720	MS.gene60093	PPI
MS.gene31196	MS.gene035720	PPI
MS.gene035720	MS.gene061869	PPI
MS.gene010219	MS.gene035720	PPI
MS.gene04052	MS.gene035720	PPI
MS.gene08122	MS.gene035720	PPI
MS.gene060465	MS.gene035720	PPI
MS.gene05623	MS.gene035720	PPI
MS.gene043967	MS.gene035720	PPI
MS.gene035720	MS.gene04972	PPI
MS.gene035720	MS.gene004121	PPI
MS.gene035720	MS.gene018578	PPI
MS.gene035720	MS.gene061867	PPI
MS.gene035720	MS.gene018580	PPI
MS.gene035720	MS.gene004124	PPI
MS.gene71215	MS.gene035720	PPI
MS.gene061866	MS.gene035720	PPI
MS.gene061870	MS.gene035720	PPI
MS.gene035720	MS.gene05619	PPI
MS.gene035720	MS.gene04052	PPI
MS.gene035718	MS.gene035720	PPI
MS.gene035720	MS.gene89488	PPI
MS.gene035720	MS.gene061866	PPI
MS.gene008276	MS.gene035720	PPI
MS.gene071136	MS.gene035720	PPI
MS.gene035720	MS.gene048705	PPI
MS.gene012390	MS.gene035720	PPI
MS.gene09873	MS.gene035720	PPI
MS.gene035720	MS.gene061874	PPI
MS.gene035720	MS.gene04576	PPI
MS.gene78578	MS.gene035720	PPI
MS.gene035720	MS.gene012390	PPI
MS.gene79195	MS.gene035720	PPI
MS.gene035721	MS.gene035720	PPI
MS.gene048705	MS.gene035720	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035720.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035720.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035720.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035720.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035720.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035720.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035720.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035720.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035720.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035720.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035720.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035720.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035720.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035720.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035720.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene035720.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035720.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035720.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035720.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035720.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035720.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035720.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035720.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035720.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035720.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035720.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035720.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 30 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTCTCCGCGACAACATTC+AGG	0.329692	8.4:-6171236	MS.gene035720:CDS
GGCAAAGGAGGAAAGGGTTT+AGG	0.384869	8.4:-6171286	MS.gene035720:CDS
GGAGGAAAGGGTTTAGGAAA+GGG	0.429069	8.4:-6171280	MS.gene035720:CDS
GGAAGGACTCTTTACGGATT+CGG	0.436444	8.4:-6171016	MS.gene035720:CDS
AGGCAGGGAAGGACTCTTTA+CGG	0.443617	8.4:-6171022	MS.gene035720:CDS
TCAACTTTCGCAGCTATGTC+AGG	0.463992	8.4:-6171313	None:intergenic
TGTTTACGCTCTCAAGAGGC+AGG	0.493747	8.4:-6171038	MS.gene035720:CDS
TGTTGTCGCGGAGAACCTTA+CGG	0.502023	8.4:+6171242	None:intergenic
AGGAAGAGGCAAAGGAGGAA+AGG	0.518412	8.4:-6171293	MS.gene035720:CDS
GGAAGAGGCAAAGGAGGAAA+GGG	0.527098	8.4:-6171292	MS.gene035720:CDS
AGGAGGAAAGGGTTTAGGAA+AGG	0.528235	8.4:-6171281	MS.gene035720:CDS
GGAAAGGGTTTAGGAAAGGG+AGG	0.536831	8.4:-6171277	MS.gene035720:CDS
CCATCGCAGTAACAGTCTTA+CGG	0.545551	8.4:+6171065	None:intergenic
GTTCTCCGCGACAACATTCA+GGG	0.554706	8.4:-6171235	MS.gene035720:CDS
CCGTAAGACTGTTACTGCGA+TGG	0.561095	8.4:-6171065	MS.gene035720:CDS
TTCGCAGCTATGTCAGGAAG+AGG	0.562303	8.4:-6171307	None:intergenic
GCTATGTCAGGAAGAGGCAA+AGG	0.571124	8.4:-6171301	MS.gene035720:CDS
AGGAGCAAAACGACACCGTA+AGG	0.575994	8.4:-6171257	MS.gene035720:CDS
ACGCTCTCAAGAGGCAGGGA+AGG	0.583359	8.4:-6171033	MS.gene035720:CDS
CTTGCAAGACGACGAATCGC+AGG	0.588436	8.4:+6171202	None:intergenic
ACGGCGAGCATGCTCTGTGT+AGG	0.590446	8.4:+6171084	None:intergenic
ATGTCAGGAAGAGGCAAAGG+AGG	0.592343	8.4:-6171298	MS.gene035720:CDS
AGGACTCTTTACGGATTCGG+TGG	0.600535	8.4:-6171013	MS.gene035720:CDS
ATGTTGTTTACGCTCTCAAG+AGG	0.616421	8.4:-6171042	MS.gene035720:CDS
GTTTACGCTCTCAAGAGGCA+GGG	0.624823	8.4:-6171037	MS.gene035720:CDS
CGTCGTCTTGCAAGACGTGG+TGG	0.633046	8.4:-6171193	MS.gene035720:CDS
TTAATCTATGAAGAAACTCG+TGG	0.634992	8.4:-6171151	MS.gene035720:CDS
TAATTCCCTGAATGTTGTCG+CGG	0.640736	8.4:+6171230	None:intergenic
ATTCGTCGTCTTGCAAGACG+TGG	0.646636	8.4:-6171196	MS.gene035720:CDS
GGTGGTGTGAAACGTATCAG+TGG	0.722771	8.4:-6171175	MS.gene035720:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTAATCTATGAAGAAACTCG+TGG	-	chr8.4:6171156-6171175	MS.gene035720:CDS	30.0%
	TAATTCCCTGAATGTTGTCG+CGG	+	chr8.4:6171080-6171099	None:intergenic	40.0%
!	ATGTTGTTTACGCTCTCAAG+AGG	-	chr8.4:6171265-6171284	MS.gene035720:CDS	40.0%
	AGGAGGAAAGGGTTTAGGAA+AGG	-	chr8.4:6171026-6171045	MS.gene035720:CDS	45.0%
	CCATCGCAGTAACAGTCTTA+CGG	+	chr8.4:6171245-6171264	None:intergenic	45.0%
	GGAAGGACTCTTTACGGATT+CGG	-	chr8.4:6171291-6171310	MS.gene035720:CDS	45.0%
	GGAGGAAAGGGTTTAGGAAA+GGG	-	chr8.4:6171027-6171046	MS.gene035720:CDS	45.0%
	AGGAAGAGGCAAAGGAGGAA+AGG	-	chr8.4:6171014-6171033	MS.gene035720:CDS	50.0%
	AGGACTCTTTACGGATTCGG+TGG	-	chr8.4:6171294-6171313	MS.gene035720:CDS	50.0%
	AGGAGCAAAACGACACCGTA+AGG	-	chr8.4:6171050-6171069	MS.gene035720:CDS	50.0%
	AGGCAGGGAAGGACTCTTTA+CGG	-	chr8.4:6171285-6171304	MS.gene035720:CDS	50.0%
	ATGTCAGGAAGAGGCAAAGG+AGG	-	chr8.4:6171009-6171028	MS.gene035720:CDS	50.0%
	ATTCGTCGTCTTGCAAGACG+TGG	-	chr8.4:6171111-6171130	MS.gene035720:CDS	50.0%
	CCGTAAGACTGTTACTGCGA+TGG	-	chr8.4:6171242-6171261	MS.gene035720:CDS	50.0%
	GGAAAGGGTTTAGGAAAGGG+AGG	-	chr8.4:6171030-6171049	MS.gene035720:CDS	50.0%
	GGAAGAGGCAAAGGAGGAAA+GGG	-	chr8.4:6171015-6171034	MS.gene035720:CDS	50.0%
	GGCAAAGGAGGAAAGGGTTT+AGG	-	chr8.4:6171021-6171040	MS.gene035720:CDS	50.0%
	GGTGGTGTGAAACGTATCAG+TGG	-	chr8.4:6171132-6171151	MS.gene035720:CDS	50.0%
	GTTCTCCGCGACAACATTCA+GGG	-	chr8.4:6171072-6171091	MS.gene035720:CDS	50.0%
	GTTTACGCTCTCAAGAGGCA+GGG	-	chr8.4:6171270-6171289	MS.gene035720:CDS	50.0%
	TGTTGTCGCGGAGAACCTTA+CGG	+	chr8.4:6171068-6171087	None:intergenic	50.0%
	TGTTTACGCTCTCAAGAGGC+AGG	-	chr8.4:6171269-6171288	MS.gene035720:CDS	50.0%
	CTTGCAAGACGACGAATCGC+AGG	+	chr8.4:6171108-6171127	None:intergenic	55.0%
	GGTTCTCCGCGACAACATTC+AGG	-	chr8.4:6171071-6171090	MS.gene035720:CDS	55.0%
	ACGCTCTCAAGAGGCAGGGA+AGG	-	chr8.4:6171274-6171293	MS.gene035720:CDS	60.0%
	ACGGCGAGCATGCTCTGTGT+AGG	+	chr8.4:6171226-6171245	None:intergenic	60.0%
	CGTCGTCTTGCAAGACGTGG+TGG	-	chr8.4:6171114-6171133	MS.gene035720:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	6171009	6171320	6171009	ID=MS.gene035720
chr8.4	mRNA	6171009	6171320	6171009	ID=MS.gene035720.t1;Parent=MS.gene035720
chr8.4	exon	6171009	6171320	6171009	ID=MS.gene035720.t1.exon1;Parent=MS.gene035720.t1
chr8.4	CDS	6171009	6171320	6171009	ID=cds.MS.gene035720.t1;Parent=MS.gene035720.t1

Gene Sequence	>MS.gene035720 ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTTTAGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTCCGCGACAACATTCAGGGAATTACAAAGCCTGCGATTCGTCGTCTTGCAAGACGTGGTGGTGTGAAACGTATCAGTGGCTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAATGTGATTCGTGATGCTGTCACCTACACAGAGCATGCTCGCCGTAAGACTGTTACTGCGATGGATGTTGTTTACGCTCTCAAGAGGCAGGGAAGGACTCTTTACGGATTCGGTGGTTAG
Protein sequence	>MS.gene035720.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG