AlfalfaGEDB | MS.gene016624

M. sativa cultivar XinJiangDaYe / MS.gene016624

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016624.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016624.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016624.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene061870	MS.gene016624	PPI
MS.gene004121	MS.gene016624	PPI
MS.gene035719	MS.gene016624	PPI
MS.gene58854	MS.gene016624	PPI
MS.gene66225	MS.gene016624	PPI
MS.gene70955	MS.gene016624	PPI
MS.gene071136	MS.gene016624	PPI
MS.gene040383	MS.gene016624	PPI
MS.gene070242	MS.gene016624	PPI
MS.gene012390	MS.gene016624	PPI
MS.gene048746	MS.gene016624	PPI
MS.gene79195	MS.gene016624	PPI
MS.gene016624	MS.gene89488	PPI
MS.gene88680	MS.gene016624	PPI
MS.gene056215	MS.gene016624	PPI
MS.gene061869	MS.gene016624	PPI
MS.gene064557	MS.gene016624	PPI
MS.gene049821	MS.gene016624	PPI
MS.gene62520	MS.gene016624	PPI
MS.gene038469	MS.gene016624	PPI
MS.gene61017	MS.gene016624	PPI
MS.gene04452	MS.gene016624	PPI
MS.gene016624	MS.gene049705	PPI
MS.gene64650	MS.gene016624	PPI
MS.gene049705	MS.gene016624	PPI
MS.gene035787	MS.gene016624	PPI
MS.gene09871	MS.gene016624	PPI
MS.gene04576	MS.gene016624	PPI
MS.gene89487	MS.gene016624	PPI
MS.gene016624	MS.gene68545	PPI
MS.gene04392	MS.gene016624	PPI
MS.gene072493	MS.gene016624	PPI
MS.gene035720	MS.gene016624	PPI
MS.gene30920	MS.gene016624	PPI
MS.gene010220	MS.gene016624	PPI
MS.gene08053	MS.gene016624	PPI
MS.gene061872	MS.gene016624	PPI
MS.gene04068	MS.gene016624	PPI
MS.gene68545	MS.gene016624	PPI
MS.gene070241	MS.gene016624	PPI
MS.gene04434	MS.gene016624	PPI
MS.gene022946	MS.gene016624	PPI
MS.gene018580	MS.gene016624	PPI
MS.gene04064	MS.gene016624	PPI
MS.gene061876	MS.gene016624	PPI
MS.gene05619	MS.gene016624	PPI
MS.gene05608	MS.gene016624	PPI
MS.gene022932	MS.gene016624	PPI
MS.gene012391	MS.gene016624	PPI
MS.gene88681	MS.gene016624	PPI
MS.gene070244	MS.gene016624	PPI
MS.gene035897	MS.gene016624	PPI
MS.gene64920	MS.gene016624	PPI
MS.gene060463	MS.gene016624	PPI
MS.gene60093	MS.gene016624	PPI
MS.gene004124	MS.gene016624	PPI
MS.gene018578	MS.gene016624	PPI
MS.gene31196	MS.gene016624	PPI
MS.gene89488	MS.gene016624	PPI
MS.gene010219	MS.gene016624	PPI
MS.gene060465	MS.gene016624	PPI
MS.gene016624	MS.gene60093	PPI
MS.gene061867	MS.gene016624	PPI
MS.gene016624	MS.gene04972	PPI
MS.gene08121	MS.gene016624	PPI
MS.gene016624	MS.gene004121	PPI
MS.gene016624	MS.gene018578	PPI
MS.gene035718	MS.gene016624	PPI
MS.gene035896	MS.gene016624	PPI
MS.gene73136	MS.gene016624	PPI
MS.gene016624	MS.gene018580	PPI
MS.gene016624	MS.gene004124	PPI
MS.gene008276	MS.gene016624	PPI
MS.gene070907	MS.gene016624	PPI
MS.gene009027	MS.gene016624	PPI
MS.gene09873	MS.gene016624	PPI
MS.gene78578	MS.gene016624	PPI
MS.gene035721	MS.gene016624	PPI
MS.gene04052	MS.gene016624	PPI
MS.gene08122	MS.gene016624	PPI
MS.gene048705	MS.gene016624	PPI
MS.gene05623	MS.gene016624	PPI
MS.gene016624	MS.gene64650	PPI
MS.gene043967	MS.gene016624	PPI
MS.gene016624	MS.gene061874	PPI
MS.gene84536	MS.gene016624	PPI
MS.gene04972	MS.gene016624	PPI
MS.gene028629	MS.gene016624	PPI
MS.gene82546	MS.gene016624	PPI
MS.gene04433	MS.gene016624	PPI
MS.gene04067	MS.gene016624	PPI
MS.gene71215	MS.gene016624	PPI
MS.gene061874	MS.gene016624	PPI
MS.gene061866	MS.gene016624	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016624.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene016624.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene016624.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene016624.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene016624.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene016624.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene016624.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene016624.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene016624.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene016624.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene016624.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene016624.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene016624.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene016624.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene016624.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene016624.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016624.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene016624.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene016624.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene016624.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene016624.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene016624.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene016624.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene016624.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene016624.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene016624.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene016624.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 30 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAAGGACTTTGTATGGTTT+CGG	0.343848	8.2:+61551733	MS.gene016624:CDS
TCGCTGGCTTTGTGATTCCT+TGG	0.395127	8.2:-61551531	None:intergenic
TTTCTTGGAGAATGTGATAA+GGG	0.404337	8.2:+61551633	MS.gene016624:CDS
GGGAGGTAAGGGACTAGGAA+AGG	0.428277	8.2:+61551468	MS.gene016624:CDS
AGACAAGGAAGGACTTTGTA+TGG	0.433181	8.2:+61551727	MS.gene016624:CDS
CTCGCTAATCGACGAATCGC+TGG	0.450047	8.2:-61551547	None:intergenic
GGAGGTAAGGGACTAGGAAA+GGG	0.452874	8.2:+61551469	MS.gene016624:CDS
GAGGAAGACGGTTACTGCTA+TGG	0.485067	8.2:+61551684	MS.gene016624:CDS
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	8.2:+61551456	MS.gene016624:CDS
GATTCTCCATTGAAAATGTC+AGG	0.496148	8.2:+61551436	None:intergenic
GGAAAGGGAGGTAAGGGACT+AGG	0.521051	8.2:+61551463	MS.gene016624:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.538992	8.2:+61551448	None:intergenic
CCATTGAAAATGTCAGGTCG+TGG	0.548627	8.2:+61551442	None:intergenic
GGTAAGGGACTAGGAAAGGG+AGG	0.563878	8.2:+61551472	MS.gene016624:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	8.2:+61551457	MS.gene016624:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	8.2:+61551447	None:intergenic
CGTCGATTAGCGAGAAGAGG+AGG	0.585804	8.2:+61551556	MS.gene016624:CDS
AGGACTTTGTATGGTTTCGG+AGG	0.599906	8.2:+61551736	MS.gene016624:CDS
ATGCTTTGAAGAGACAAGGA+AGG	0.603293	8.2:+61551716	MS.gene016624:CDS
ATTCGTCGATTAGCGAGAAG+AGG	0.605618	8.2:+61551553	MS.gene016624:CDS
GTGCTTCGCGACAACATCCA+AGG	0.609211	8.2:+61551514	MS.gene016624:CDS
GGAGGTGTGAAGAGAATCAG+CGG	0.613587	8.2:+61551574	MS.gene016624:CDS
GTTTATGCTTTGAAGAGACA+AGG	0.627217	8.2:+61551712	MS.gene016624:CDS
TGAACATGCGAGGAGGAAGA+CGG	0.628798	8.2:+61551672	MS.gene016624:CDS
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	8.2:+61551451	MS.gene016624:CDS
GAGGTGTGAAGAGAATCAGC+GGG	0.664332	8.2:+61551575	MS.gene016624:CDS
TAGGAAAGGGAGGTGCAAAG+AGG	0.683932	8.2:+61551482	MS.gene016624:CDS
TTGATTTATGAAGAAACTCG+CGG	0.688782	8.2:+61551598	MS.gene016624:CDS
CGTATACTGAACATGCGAGG+AGG	0.708048	8.2:+61551665	MS.gene016624:CDS
TTACGTATACTGAACATGCG+AGG	0.714123	8.2:+61551662	MS.gene016624:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATTTATGAAGAAACTCG+CGG	+	chr8.2:61551598-61551617	MS.gene016624:CDS	30.0%
	TTTCTTGGAGAATGTGATAA+GGG	+	chr8.2:61551633-61551652	MS.gene016624:CDS	30.0%
!	TTTTCTTGGAGAATGTGATA+AGG	+	chr8.2:61551632-61551651	MS.gene016624:CDS	30.0%
!!	GTTTATGCTTTGAAGAGACA+AGG	+	chr8.2:61551712-61551731	MS.gene016624:CDS	35.0%
	AGACAAGGAAGGACTTTGTA+TGG	+	chr8.2:61551727-61551746	MS.gene016624:CDS	40.0%
	GGAAGGACTTTGTATGGTTT+CGG	+	chr8.2:61551733-61551752	MS.gene016624:CDS	40.0%
	TTACGTATACTGAACATGCG+AGG	+	chr8.2:61551662-61551681	MS.gene016624:CDS	40.0%
!	CGGTGTGTTGAAGATTTTCT+TGG	+	chr8.2:61551618-61551637	MS.gene016624:CDS	40.0%
!!	ATGCTTTGAAGAGACAAGGA+AGG	+	chr8.2:61551716-61551735	MS.gene016624:CDS	40.0%
	AGGACTTTGTATGGTTTCGG+AGG	+	chr8.2:61551736-61551755	MS.gene016624:CDS	45.0%
!	ATTCGTCGATTAGCGAGAAG+AGG	+	chr8.2:61551553-61551572	MS.gene016624:CDS	45.0%
	CGTATACTGAACATGCGAGG+AGG	+	chr8.2:61551665-61551684	MS.gene016624:CDS	50.0%
	GAGGAAGACGGTTACTGCTA+TGG	+	chr8.2:61551684-61551703	MS.gene016624:CDS	50.0%
	GAGGTGTGAAGAGAATCAGC+GGG	+	chr8.2:61551575-61551594	MS.gene016624:CDS	50.0%
	GGAGGTAAGGGACTAGGAAA+GGG	+	chr8.2:61551469-61551488	MS.gene016624:CDS	50.0%
	TGAACATGCGAGGAGGAAGA+CGG	+	chr8.2:61551672-61551691	MS.gene016624:CDS	50.0%
!	GGAGGTGTGAAGAGAATCAG+CGG	+	chr8.2:61551574-61551593	MS.gene016624:CDS	50.0%
!	TAGGAAAGGGAGGTGCAAAG+AGG	+	chr8.2:61551482-61551501	MS.gene016624:CDS	50.0%
!!	TCGCTGGCTTTGTGATTCCT+TGG	-	chr8.2:61551534-61551553	None:intergenic	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	+	chr8.2:61551456-61551475	MS.gene016624:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	+	chr8.2:61551451-61551470	MS.gene016624:CDS	55.0%
	CTCGCTAATCGACGAATCGC+TGG	-	chr8.2:61551550-61551569	None:intergenic	55.0%
	GGAAAGGGAGGTAAGGGACT+AGG	+	chr8.2:61551463-61551482	MS.gene016624:CDS	55.0%
	GGGAGGTAAGGGACTAGGAA+AGG	+	chr8.2:61551468-61551487	MS.gene016624:CDS	55.0%
	GGTAAGGGACTAGGAAAGGG+AGG	+	chr8.2:61551472-61551491	MS.gene016624:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	+	chr8.2:61551457-61551476	MS.gene016624:CDS	55.0%
	GTGCTTCGCGACAACATCCA+AGG	+	chr8.2:61551514-61551533	MS.gene016624:CDS	55.0%
!	CGTCGATTAGCGAGAAGAGG+AGG	+	chr8.2:61551556-61551575	MS.gene016624:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	61551451	61551762	61551451	ID=MS.gene016624
chr8.2	mRNA	61551451	61551762	61551451	ID=MS.gene016624.t1;Parent=MS.gene016624
chr8.2	exon	61551451	61551762	61551451	ID=MS.gene016624.t1.exon1;Parent=MS.gene016624.t1
chr8.2	CDS	61551451	61551762	61551451	ID=cds.MS.gene016624.t1;Parent=MS.gene016624.t1

Gene Sequence	>MS.gene016624 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTAGGAAAGGGAGGTGCAAAGAGGCATCGCAAAGTGCTTCGCGACAACATCCAAGGAATCACAAAGCCAGCGATTCGTCGATTAGCGAGAAGAGGAGGTGTGAAGAGAATCAGCGGGTTGATTTATGAAGAAACTCGCGGTGTGTTGAAGATTTTCTTGGAGAATGTGATAAGGGATGCTGTTACGTATACTGAACATGCGAGGAGGAAGACGGTTACTGCTATGGATGTTGTTTATGCTTTGAAGAGACAAGGAAGGACTTTGTATGGTTTCGGAGGTTGA
Protein sequence	>MS.gene016624.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG