AlfalfaGEDB | MS.gene035896

M. sativa cultivar XinJiangDaYe / MS.gene035896

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035896.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035896.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035896.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene08121	MS.gene035896	PPI
MS.gene022932	MS.gene035896	PPI
MS.gene012391	MS.gene035896	PPI
MS.gene88681	MS.gene035896	PPI
MS.gene035896	MS.gene072493	PPI
MS.gene070244	MS.gene035896	PPI
MS.gene035896	MS.gene84536	PPI
MS.gene64920	MS.gene035896	PPI
MS.gene035896	MS.gene82546	PPI
MS.gene009027	MS.gene035896	PPI
MS.gene035896	MS.gene60093	PPI
MS.gene035896	MS.gene08122	PPI
MS.gene31196	MS.gene035896	PPI
MS.gene035896	MS.gene022932	PPI
MS.gene060465	MS.gene035896	PPI
MS.gene043967	MS.gene035896	PPI
MS.gene035896	MS.gene010220	PPI
MS.gene035896	MS.gene04972	PPI
MS.gene035896	MS.gene004121	PPI
MS.gene035896	MS.gene62520	PPI
MS.gene035896	MS.gene018578	PPI
MS.gene035896	MS.gene04064	PPI
MS.gene035896	MS.gene018580	PPI
MS.gene035896	MS.gene004124	PPI
MS.gene71215	MS.gene035896	PPI
MS.gene061870	MS.gene035896	PPI
MS.gene035896	MS.gene05619	PPI
MS.gene035896	MS.gene04052	PPI
MS.gene035718	MS.gene035896	PPI
MS.gene035896	MS.gene061866	PPI
MS.gene071136	MS.gene035896	PPI
MS.gene035896	MS.gene64650	PPI
MS.gene035896	MS.gene79195	PPI
MS.gene035896	MS.gene048705	PPI
MS.gene035896	MS.gene061874	PPI
MS.gene035896	MS.gene04576	PPI
MS.gene78578	MS.gene035896	PPI
MS.gene035896	MS.gene061869	PPI
MS.gene035721	MS.gene035896	PPI
MS.gene064557	MS.gene035896	PPI
MS.gene038469	MS.gene035896	PPI
MS.gene028629	MS.gene035896	PPI
MS.gene04433	MS.gene035896	PPI
MS.gene04452	MS.gene035896	PPI
MS.gene035896	MS.gene061867	PPI
MS.gene035896	MS.gene008276	PPI
MS.gene035787	MS.gene035896	PPI
MS.gene04067	MS.gene035896	PPI
MS.gene035896	MS.gene73136	PPI
MS.gene89487	MS.gene035896	PPI
MS.gene035896	MS.gene049821	PPI
MS.gene035896	MS.gene04068	PPI
MS.gene035719	MS.gene035896	PPI
MS.gene035896	MS.gene022946	PPI
MS.gene035896	MS.gene05623	PPI
MS.gene58854	MS.gene035896	PPI
MS.gene035896	MS.gene89488	PPI
MS.gene66225	MS.gene035896	PPI
MS.gene70955	MS.gene035896	PPI
MS.gene040383	MS.gene035896	PPI
MS.gene035896	MS.gene66225	PPI
MS.gene070242	MS.gene035896	PPI
MS.gene035896	MS.gene035720	PPI
MS.gene035896	MS.gene61017	PPI
MS.gene010220	MS.gene035896	PPI
MS.gene061872	MS.gene035896	PPI
MS.gene035896	MS.gene09871	PPI
MS.gene035896	MS.gene012390	PPI
MS.gene88680	MS.gene035896	PPI
MS.gene070241	MS.gene035896	PPI
MS.gene035896	MS.gene049705	PPI
MS.gene056215	MS.gene035896	PPI
MS.gene035896	MS.gene04434	PPI
MS.gene035896	MS.gene070907	PPI
MS.gene035896	MS.gene04392	PPI
MS.gene035896	MS.gene010219	PPI
MS.gene035896	MS.gene05608	PPI
MS.gene061876	MS.gene035896	PPI
MS.gene035896	MS.gene68545	PPI
MS.gene035896	MS.gene070242	PPI
MS.gene035896	MS.gene016624	PPI
MS.gene060463	MS.gene035896	PPI
MS.gene035896	MS.gene048746	PPI
MS.gene035896	MS.gene09873	PPI
MS.gene30920	MS.gene035896	PPI
MS.gene08053	MS.gene035896	PPI
MS.gene035896	MS.gene043967	PPI
MS.gene035896	MS.gene035897	PPI
MS.gene035896	MS.gene035721	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035896.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035896.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035896.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035896.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035896.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035896.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035896.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035896.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035896.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035896.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035896.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035896.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035896.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035896.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035896.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene035896.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035896.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035896.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035896.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035896.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035896.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035896.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035896.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035896.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035896.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035896.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035896.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 30 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGTTGTTTATGCACTTAAA+AGG	0.251343	5.2:-53279713	MS.gene035896:CDS
AGGCAAGGAAGGACCCTTTA+TGG	0.274450	5.2:-53279693	MS.gene035896:CDS
GGAAGGACCCTTTATGGTTT+CGG	0.347466	5.2:-53279687	MS.gene035896:CDS
CCTAACCACCGAAACCATAA+AGG	0.360537	5.2:+53279679	None:intergenic
CCTTTATGGTTTCGGTGGTT+AGG	0.360991	5.2:-53279679	None:intergenic
GGCAAAGGAGGAAAGGGTTT+AGG	0.384869	5.2:-53279957	MS.gene035896:CDS
GTTCTTCGAGATAACATTCA+AGG	0.421661	5.2:-53279906	MS.gene035896:CDS
TCGCAAAACGGTTACTGCTA+TGG	0.471375	5.2:-53279736	MS.gene035896:CDS
ATCTTGCAATCAACAATGTC+AGG	0.473641	5.2:-53279984	None:intergenic
CTAACCACCGAAACCATAAA+GGG	0.505014	5.2:+53279680	None:intergenic
GGAGGAAAGGGTTTAGGAAA+GGG	0.517349	5.2:-53279951	MS.gene035896:CDS
TGAACATGCTCGTCGCAAAA+CGG	0.517772	5.2:-53279748	MS.gene035896:CDS
AGGAAGAGGCAAAGGAGGAA+AGG	0.518412	5.2:-53279964	MS.gene035896:CDS
CCGCGATTCGTCGTCTTGCA+AGG	0.525617	5.2:-53279872	MS.gene035896:CDS
GGAAGAGGCAAAGGAGGAAA+GGG	0.527098	5.2:-53279963	MS.gene035896:CDS
CTTGCAAGACGACGAATCGC+GGG	0.554223	5.2:+53279873	None:intergenic
GTTTATGCACTTAAAAGGCA+AGG	0.566728	5.2:-53279708	MS.gene035896:CDS
CCTTGCAAGACGACGAATCG+CGG	0.571292	5.2:+53279872	None:intergenic
CAATCAACAATGTCAGGAAG+AGG	0.577531	5.2:-53279978	None:intergenic
ATGCACTTAAAAGGCAAGGA+AGG	0.580160	5.2:-53279704	MS.gene035896:CDS
AGGACCCTTTATGGTTTCGG+TGG	0.597859	5.2:-53279684	MS.gene035896:CDS
ACAATGTCAGGAAGAGGCAA+AGG	0.598135	5.2:-53279972	MS.gene035896:CDS
ATGTCAGGAAGAGGCAAAGG+AGG	0.604597	5.2:-53279969	MS.gene035896:CDS
CGTCGTCTTGCAAGGCGTGG+TGG	0.615268	5.2:-53279864	MS.gene035896:CDS
AGGAGGAAAGGGTTTAGGAA+AGG	0.616515	5.2:-53279952	MS.gene035896:CDS
ATTCGTCGTCTTGCAAGGCG+TGG	0.619919	5.2:-53279867	MS.gene035896:CDS
CTTATCTATGAAGAAACTCG+TGG	0.644041	5.2:-53279822	MS.gene035896:CDS
GGTGGTGTGAAGCGTATCAG+TGG	0.661880	5.2:-53279846	MS.gene035896:CDS
TATCTCGAAGAACTTTGCGA+TGG	0.662547	5.2:+53279916	None:intergenic
GGAAAGGGTTTAGGAAAGGG+CGG	0.700891	5.2:-53279948	MS.gene035896:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATGTTGTTTATGCACTTAAA+AGG	-	chr5.2:53279936-53279955	MS.gene035896:CDS	25.0%
	CTTATCTATGAAGAAACTCG+TGG	-	chr5.2:53279827-53279846	MS.gene035896:CDS	35.0%
	GTTCTTCGAGATAACATTCA+AGG	-	chr5.2:53279743-53279762	MS.gene035896:CDS	35.0%
	GTTTATGCACTTAAAAGGCA+AGG	-	chr5.2:53279941-53279960	MS.gene035896:CDS	35.0%
	ATGCACTTAAAAGGCAAGGA+AGG	-	chr5.2:53279945-53279964	MS.gene035896:CDS	40.0%
	CTAACCACCGAAACCATAAA+GGG	+	chr5.2:53279972-53279991	None:intergenic	40.0%
	TATCTCGAAGAACTTTGCGA+TGG	+	chr5.2:53279736-53279755	None:intergenic	40.0%
	AGGAGGAAAGGGTTTAGGAA+AGG	-	chr5.2:53279697-53279716	MS.gene035896:CDS	45.0%
	GGAAGGACCCTTTATGGTTT+CGG	-	chr5.2:53279962-53279981	MS.gene035896:CDS	45.0%
	GGAGGAAAGGGTTTAGGAAA+GGG	-	chr5.2:53279698-53279717	MS.gene035896:CDS	45.0%
	TCGCAAAACGGTTACTGCTA+TGG	-	chr5.2:53279913-53279932	MS.gene035896:CDS	45.0%
	TGAACATGCTCGTCGCAAAA+CGG	-	chr5.2:53279901-53279920	MS.gene035896:CDS	45.0%
	AGGAAGAGGCAAAGGAGGAA+AGG	-	chr5.2:53279685-53279704	MS.gene035896:CDS	50.0%
	AGGACCCTTTATGGTTTCGG+TGG	-	chr5.2:53279965-53279984	MS.gene035896:CDS	50.0%
	AGGCAAGGAAGGACCCTTTA+TGG	-	chr5.2:53279956-53279975	MS.gene035896:CDS	50.0%
	ATGTCAGGAAGAGGCAAAGG+AGG	-	chr5.2:53279680-53279699	MS.gene035896:CDS	50.0%
	GGAAAGGGTTTAGGAAAGGG+CGG	-	chr5.2:53279701-53279720	MS.gene035896:CDS	50.0%
	GGAAGAGGCAAAGGAGGAAA+GGG	-	chr5.2:53279686-53279705	MS.gene035896:CDS	50.0%
	GGCAAAGGAGGAAAGGGTTT+AGG	-	chr5.2:53279692-53279711	MS.gene035896:CDS	50.0%
	ATTCGTCGTCTTGCAAGGCG+TGG	-	chr5.2:53279782-53279801	MS.gene035896:CDS	55.0%
	CCTTGCAAGACGACGAATCG+CGG	+	chr5.2:53279780-53279799	None:intergenic	55.0%
	CTTGCAAGACGACGAATCGC+GGG	+	chr5.2:53279779-53279798	None:intergenic	55.0%
	GGTGGTGTGAAGCGTATCAG+TGG	-	chr5.2:53279803-53279822	MS.gene035896:CDS	55.0%
	CCGCGATTCGTCGTCTTGCA+AGG	-	chr5.2:53279777-53279796	MS.gene035896:CDS	60.0%
	CGTCGTCTTGCAAGGCGTGG+TGG	-	chr5.2:53279785-53279804	MS.gene035896:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	53279680	53279991	53279680	ID=MS.gene035896
chr5.2	mRNA	53279680	53279991	53279680	ID=MS.gene035896.t1;Parent=MS.gene035896
chr5.2	exon	53279680	53279991	53279680	ID=MS.gene035896.t1.exon1;Parent=MS.gene035896.t1
chr5.2	CDS	53279680	53279991	53279680	ID=cds.MS.gene035896.t1;Parent=MS.gene035896.t1

Gene Sequence	>MS.gene035896 ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTTTAGGAAAGGGCGGTGCAAAGCGCCATCGCAAAGTTCTTCGAGATAACATTCAAGGAATAACGAAACCCGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTGAAGCGTATCAGTGGTCTTATCTATGAAGAAACTCGTGGTGTTCTTAAGATATTTCTTGAGAATGTTATTCGCGATGCTGTCACTTACACTGAACATGCTCGTCGCAAAACGGTTACTGCTATGGATGTTGTTTATGCACTTAAAAGGCAAGGAAGGACCCTTTATGGTTTCGGTGGTTAG
Protein sequence	>MS.gene035896.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG