AlfalfaGEDB | MS.gene84536

M. sativa cultivar XinJiangDaYe / MS.gene84536

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84536.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84536.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84536.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene08121	MS.gene84536	PPI
MS.gene022932	MS.gene84536	PPI
MS.gene84536	MS.gene035720	PPI
MS.gene012391	MS.gene84536	PPI
MS.gene88681	MS.gene84536	PPI
MS.gene070244	MS.gene84536	PPI
MS.gene035897	MS.gene84536	PPI
MS.gene035896	MS.gene84536	PPI
MS.gene73136	MS.gene84536	PPI
MS.gene64920	MS.gene84536	PPI
MS.gene070907	MS.gene84536	PPI
MS.gene84536	MS.gene049705	PPI
MS.gene009027	MS.gene84536	PPI
MS.gene31196	MS.gene84536	PPI
MS.gene010219	MS.gene84536	PPI
MS.gene04052	MS.gene84536	PPI
MS.gene84536	MS.gene05608	PPI
MS.gene060465	MS.gene84536	PPI
MS.gene05623	MS.gene84536	PPI
MS.gene84536	MS.gene60093	PPI
MS.gene84536	MS.gene09873	PPI
MS.gene061870	MS.gene84536	PPI
MS.gene035718	MS.gene84536	PPI
MS.gene84536	MS.gene04972	PPI
MS.gene008276	MS.gene84536	PPI
MS.gene84536	MS.gene62520	PPI
MS.gene84536	MS.gene018578	PPI
MS.gene012390	MS.gene84536	PPI
MS.gene09873	MS.gene84536	PPI
MS.gene84536	MS.gene018580	PPI
MS.gene84536	MS.gene004124	PPI
MS.gene78578	MS.gene84536	PPI
MS.gene035721	MS.gene84536	PPI
MS.gene08122	MS.gene84536	PPI
MS.gene048705	MS.gene84536	PPI
MS.gene043967	MS.gene84536	PPI
MS.gene064557	MS.gene84536	PPI
MS.gene038469	MS.gene84536	PPI
MS.gene84536	MS.gene04052	PPI
MS.gene028629	MS.gene84536	PPI
MS.gene84536	MS.gene061866	PPI
MS.gene82546	MS.gene84536	PPI
MS.gene61017	MS.gene84536	PPI
MS.gene04433	MS.gene84536	PPI
MS.gene04452	MS.gene84536	PPI
MS.gene84536	MS.gene64650	PPI
MS.gene84536	MS.gene048705	PPI
MS.gene84536	MS.gene061874	PPI
MS.gene035787	MS.gene84536	PPI
MS.gene04067	MS.gene84536	PPI
MS.gene71215	MS.gene84536	PPI
MS.gene061866	MS.gene84536	PPI
MS.gene84536	MS.gene061869	PPI
MS.gene89487	MS.gene84536	PPI
MS.gene035719	MS.gene84536	PPI
MS.gene58854	MS.gene84536	PPI
MS.gene84536	MS.gene004121	PPI
MS.gene66225	MS.gene84536	PPI
MS.gene70955	MS.gene84536	PPI
MS.gene071136	MS.gene84536	PPI
MS.gene040383	MS.gene84536	PPI
MS.gene070242	MS.gene84536	PPI
MS.gene072493	MS.gene84536	PPI
MS.gene035720	MS.gene84536	PPI
MS.gene048746	MS.gene84536	PPI
MS.gene010220	MS.gene84536	PPI
MS.gene84536	MS.gene061867	PPI
MS.gene84536	MS.gene008276	PPI
MS.gene79195	MS.gene84536	PPI
MS.gene88680	MS.gene84536	PPI
MS.gene84536	MS.gene05619	PPI
MS.gene84536	MS.gene73136	PPI
MS.gene056215	MS.gene84536	PPI
MS.gene049821	MS.gene84536	PPI
MS.gene62520	MS.gene84536	PPI
MS.gene84536	MS.gene89488	PPI
MS.gene061876	MS.gene84536	PPI
MS.gene05608	MS.gene84536	PPI
MS.gene84536	MS.gene61017	PPI
MS.gene84536	MS.gene04576	PPI
MS.gene09871	MS.gene84536	PPI
MS.gene84536	MS.gene012390	PPI
MS.gene04576	MS.gene84536	PPI
MS.gene060463	MS.gene84536	PPI
MS.gene84536	MS.gene010219	PPI
MS.gene04392	MS.gene84536	PPI
MS.gene30920	MS.gene84536	PPI
MS.gene84536	MS.gene68545	PPI
MS.gene08053	MS.gene84536	PPI
MS.gene061872	MS.gene84536	PPI
MS.gene84536	MS.gene016624	PPI
MS.gene04068	MS.gene84536	PPI
MS.gene070241	MS.gene84536	PPI
MS.gene04434	MS.gene84536	PPI
MS.gene022946	MS.gene84536	PPI
MS.gene04064	MS.gene84536	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84536.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene84536.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene84536.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene84536.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene84536.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene84536.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene84536.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene84536.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene84536.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene84536.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene84536.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene84536.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene84536.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene84536.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene84536.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene84536.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84536.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene84536.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene84536.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene84536.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene84536.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene84536.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene84536.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene84536.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene84536.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene84536.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene84536.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 34 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCGCCAATCGACGAATTGC+AGG	0.267346	3.1:+46113707	None:intergenic
GGAAGAACCCTCTATGGATT+TGG	0.370047	3.1:-46113521	MS.gene84536:CDS
AGACAAGGAAGAACCCTCTA+TGG	0.396310	3.1:-46113527	MS.gene84536:CDS
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	3.1:-46113636	MS.gene84536:CDS
GGGAGGTAAGGGACTTGGAA+AGG	0.449159	3.1:-46113786	MS.gene84536:CDS
CTCTTGAAAATGTCAGGTCG+TGG	0.456977	3.1:-46113812	None:intergenic
GGAGGTAAGGGACTTGGAAA+GGG	0.467307	3.1:-46113785	MS.gene84536:CDS
GGAAAGGGAGGTAAGGGACT+TGG	0.470633	3.1:-46113791	MS.gene84536:CDS
TTCAACCTCCAAATCCATAG+AGG	0.476286	3.1:+46113513	None:intergenic
CAATCGACGAATTGCAGGCT+TGG	0.491738	3.1:+46113712	None:intergenic
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.1:-46113798	MS.gene84536:CDS
AGAACCCTCTATGGATTTGG+AGG	0.508817	3.1:-46113518	MS.gene84536:CDS
GAAGATCTTCAAGACACCTC+TGG	0.510902	3.1:+46113640	None:intergenic
CAAGCCTGCAATTCGTCGAT+TGG	0.511374	3.1:-46113711	MS.gene84536:CDS
TTACATACACTGAGCATGCT+AGG	0.520723	3.1:-46113592	MS.gene84536:CDS
CGTCGATTGGCGAGAAGAGG+TGG	0.521251	3.1:-46113698	MS.gene84536:CDS
AGTTCTTCGTGATAACATCC+AGG	0.522451	3.1:-46113741	MS.gene84536:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.538992	3.1:-46113806	MS.gene84536:CDS
AGGGAGGTGCAAAGAGGCAT+AGG	0.547891	3.1:-46113766	MS.gene84536:CDS
GGTGGTGTGAAGAGAATCAG+TGG	0.556654	3.1:-46113680	MS.gene84536:CDS
GGTAAGGGACTTGGAAAGGG+AGG	0.560743	3.1:-46113782	MS.gene84536:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	3.1:-46113797	MS.gene84536:CDS
GTCTATGCTTTGAAGAGACA+AGG	0.570048	3.1:-46113542	MS.gene84536:CDS
GAGGAAGACTGTGACAGCCA+TGG	0.570644	3.1:-46113570	MS.gene84536:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.1:-46113807	None:intergenic
ATTCGTCGATTGGCGAGAAG+AGG	0.610547	3.1:-46113701	MS.gene84536:CDS
TTGCAGGCTTGGTGATACCC+TGG	0.613296	3.1:+46113723	None:intergenic
CAAAGCATAGACAACATCCA+TGG	0.629842	3.1:+46113553	None:intergenic
TCAACCTCCAAATCCATAGA+GGG	0.633476	3.1:+46113514	None:intergenic
GTTCTTCGTGATAACATCCA+GGG	0.641996	3.1:-46113740	MS.gene84536:CDS
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.1:-46113803	MS.gene84536:CDS
TTGGAAAGGGAGGTGCAAAG+AGG	0.683014	3.1:-46113772	MS.gene84536:CDS
TTGATCTATGAAGAAACCAG+AGG	0.704889	3.1:-46113656	MS.gene84536:CDS
CATACACTGAGCATGCTAGG+AGG	0.707859	3.1:-46113589	MS.gene84536:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATCTATGAAGAAACCAG+AGG	-	chr3.1:46113661-46113680	MS.gene84536:CDS	35.0%
	AGGTGTCTTGAAGATCTTCT+TGG	-	chr3.1:46113681-46113700	MS.gene84536:CDS	40.0%
	AGTTCTTCGTGATAACATCC+AGG	-	chr3.1:46113576-46113595	MS.gene84536:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	+	chr3.1:46113767-46113786	None:intergenic	40.0%
	GTTCTTCGTGATAACATCCA+GGG	-	chr3.1:46113577-46113596	MS.gene84536:CDS	40.0%
	TCAACCTCCAAATCCATAGA+GGG	+	chr3.1:46113806-46113825	None:intergenic	40.0%
	TTACATACACTGAGCATGCT+AGG	-	chr3.1:46113725-46113744	MS.gene84536:CDS	40.0%
!!	GTCTATGCTTTGAAGAGACA+AGG	-	chr3.1:46113775-46113794	MS.gene84536:CDS	40.0%
	AGAACCCTCTATGGATTTGG+AGG	-	chr3.1:46113799-46113818	MS.gene84536:CDS	45.0%
	AGACAAGGAAGAACCCTCTA+TGG	-	chr3.1:46113790-46113809	MS.gene84536:CDS	45.0%
	GAAGATCTTCAAGACACCTC+TGG	+	chr3.1:46113680-46113699	None:intergenic	45.0%
	GGAAGAACCCTCTATGGATT+TGG	-	chr3.1:46113796-46113815	MS.gene84536:CDS	45.0%
	CAAGCCTGCAATTCGTCGAT+TGG	-	chr3.1:46113606-46113625	MS.gene84536:CDS	50.0%
	CAATCGACGAATTGCAGGCT+TGG	+	chr3.1:46113608-46113627	None:intergenic	50.0%
	CATACACTGAGCATGCTAGG+AGG	-	chr3.1:46113728-46113747	MS.gene84536:CDS	50.0%
	GGAGGTAAGGGACTTGGAAA+GGG	-	chr3.1:46113532-46113551	MS.gene84536:CDS	50.0%
	GGTGGTGTGAAGAGAATCAG+TGG	-	chr3.1:46113637-46113656	MS.gene84536:CDS	50.0%
!	ATTCGTCGATTGGCGAGAAG+AGG	-	chr3.1:46113616-46113635	MS.gene84536:CDS	50.0%
!	TTGGAAAGGGAGGTGCAAAG+AGG	-	chr3.1:46113545-46113564	MS.gene84536:CDS	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	-	chr3.1:46113519-46113538	MS.gene84536:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	-	chr3.1:46113514-46113533	MS.gene84536:CDS	55.0%
	CTCGCCAATCGACGAATTGC+AGG	+	chr3.1:46113613-46113632	None:intergenic	55.0%
	GAGGAAGACTGTGACAGCCA+TGG	-	chr3.1:46113747-46113766	MS.gene84536:CDS	55.0%
	GGAAAGGGAGGTAAGGGACT+TGG	-	chr3.1:46113526-46113545	MS.gene84536:CDS	55.0%
	GGGAGGTAAGGGACTTGGAA+AGG	-	chr3.1:46113531-46113550	MS.gene84536:CDS	55.0%
	GGTAAGGGACTTGGAAAGGG+AGG	-	chr3.1:46113535-46113554	MS.gene84536:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	-	chr3.1:46113520-46113539	MS.gene84536:CDS	55.0%
!	AGGGAGGTGCAAAGAGGCAT+AGG	-	chr3.1:46113551-46113570	MS.gene84536:CDS	55.0%
!!	TTGCAGGCTTGGTGATACCC+TGG	+	chr3.1:46113597-46113616	None:intergenic	55.0%
!	CGTCGATTGGCGAGAAGAGG+TGG	-	chr3.1:46113619-46113638	MS.gene84536:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	46113514	46113825	46113514	ID=MS.gene84536
chr3.1	mRNA	46113514	46113825	46113514	ID=MS.gene84536.t1;Parent=MS.gene84536
chr3.1	exon	46113514	46113825	46113514	ID=MS.gene84536.t1.exon1;Parent=MS.gene84536.t1
chr3.1	CDS	46113514	46113825	46113514	ID=cds.MS.gene84536.t1;Parent=MS.gene84536.t1

Gene Sequence	>MS.gene84536 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTTGGAAAGGGAGGTGCAAAGAGGCATAGGAAAGTTCTTCGTGATAACATCCAGGGTATCACCAAGCCTGCAATTCGTCGATTGGCGAGAAGAGGTGGTGTGAAGAGAATCAGTGGATTGATCTATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAACGTCATTCGTGATGCAGTTACATACACTGAGCATGCTAGGAGGAAGACTGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAAGGAAGAACCCTCTATGGATTTGGAGGTTGA
Protein sequence	>MS.gene84536.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG