AlfalfaGEDB | MS.gene048705

M. sativa cultivar XinJiangDaYe / MS.gene048705

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene048705.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene048705.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene048705.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene070241	MS.gene048705	PPI
MS.gene049821	MS.gene048705	PPI
MS.gene022946	MS.gene048705	PPI
MS.gene62520	MS.gene048705	PPI
MS.gene04064	MS.gene048705	PPI
MS.gene061876	MS.gene048705	PPI
MS.gene048705	MS.gene049705	PPI
MS.gene05608	MS.gene048705	PPI
MS.gene012391	MS.gene048705	PPI
MS.gene070244	MS.gene048705	PPI
MS.gene09871	MS.gene048705	PPI
MS.gene035897	MS.gene048705	PPI
MS.gene64920	MS.gene048705	PPI
MS.gene048705	MS.gene05608	PPI
MS.gene060463	MS.gene048705	PPI
MS.gene048705	MS.gene68545	PPI
MS.gene31196	MS.gene048705	PPI
MS.gene048705	MS.gene60093	PPI
MS.gene048705	MS.gene09873	PPI
MS.gene04068	MS.gene048705	PPI
MS.gene04434	MS.gene048705	PPI
MS.gene048705	MS.gene62520	PPI
MS.gene08121	MS.gene048705	PPI
MS.gene048705	MS.gene018578	PPI
MS.gene022932	MS.gene048705	PPI
MS.gene88681	MS.gene048705	PPI
MS.gene048705	MS.gene018580	PPI
MS.gene048705	MS.gene004124	PPI
MS.gene035718	MS.gene048705	PPI
MS.gene035896	MS.gene048705	PPI
MS.gene73136	MS.gene048705	PPI
MS.gene008276	MS.gene048705	PPI
MS.gene070907	MS.gene048705	PPI
MS.gene009027	MS.gene048705	PPI
MS.gene09873	MS.gene048705	PPI
MS.gene048705	MS.gene04052	PPI
MS.gene048705	MS.gene84536	PPI
MS.gene048705	MS.gene061866	PPI
MS.gene78578	MS.gene048705	PPI
MS.gene010219	MS.gene048705	PPI
MS.gene04052	MS.gene048705	PPI
MS.gene08122	MS.gene048705	PPI
MS.gene060465	MS.gene048705	PPI
MS.gene048705	MS.gene64650	PPI
MS.gene05623	MS.gene048705	PPI
MS.gene043967	MS.gene048705	PPI
MS.gene048705	MS.gene061874	PPI
MS.gene048705	MS.gene061869	PPI
MS.gene84536	MS.gene048705	PPI
MS.gene028629	MS.gene048705	PPI
MS.gene048705	MS.gene04972	PPI
MS.gene048705	MS.gene004121	PPI
MS.gene04067	MS.gene048705	PPI
MS.gene71215	MS.gene048705	PPI
MS.gene061866	MS.gene048705	PPI
MS.gene061870	MS.gene048705	PPI
MS.gene048705	MS.gene061867	PPI
MS.gene048705	MS.gene008276	PPI
MS.gene048705	MS.gene05619	PPI
MS.gene66225	MS.gene048705	PPI
MS.gene071136	MS.gene048705	PPI
MS.gene070242	MS.gene048705	PPI
MS.gene012390	MS.gene048705	PPI
MS.gene048705	MS.gene89488	PPI
MS.gene79195	MS.gene048705	PPI
MS.gene035721	MS.gene048705	PPI
MS.gene048705	MS.gene61017	PPI
MS.gene056215	MS.gene048705	PPI
MS.gene048705	MS.gene04576	PPI
MS.gene064557	MS.gene048705	PPI
MS.gene038469	MS.gene048705	PPI
MS.gene048705	MS.gene012390	PPI
MS.gene82546	MS.gene048705	PPI
MS.gene61017	MS.gene048705	PPI
MS.gene04433	MS.gene048705	PPI
MS.gene04452	MS.gene048705	PPI
MS.gene035787	MS.gene048705	PPI
MS.gene048705	MS.gene010219	PPI
MS.gene04576	MS.gene048705	PPI
MS.gene89487	MS.gene048705	PPI
MS.gene035719	MS.gene048705	PPI
MS.gene048705	MS.gene016624	PPI
MS.gene58854	MS.gene048705	PPI
MS.gene048705	MS.gene73136	PPI
MS.gene70955	MS.gene048705	PPI
MS.gene040383	MS.gene048705	PPI
MS.gene04392	MS.gene048705	PPI
MS.gene072493	MS.gene048705	PPI
MS.gene035720	MS.gene048705	PPI
MS.gene30920	MS.gene048705	PPI
MS.gene048746	MS.gene048705	PPI
MS.gene010220	MS.gene048705	PPI
MS.gene08053	MS.gene048705	PPI
MS.gene061872	MS.gene048705	PPI
MS.gene88680	MS.gene048705	PPI
MS.gene048705	MS.gene035720	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene048705.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048705.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048705.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048705.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048705.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048705.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048705.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048705.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048705.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048705.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048705.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048705.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048705.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048705.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene048705.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene048705.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene048705.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048705.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048705.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048705.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048705.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048705.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048705.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048705.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048705.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048705.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene048705.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 36 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCGCCAATCGACGAATTGC+AGG	0.267346	3.2:+60976343	None:intergenic
GGAAGAACCCTCTATGGATT+TGG	0.370047	3.2:-60976157	MS.gene048705:CDS
AGACAAGGAAGAACCCTCTA+TGG	0.396310	3.2:-60976163	MS.gene048705:CDS
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	3.2:-60976272	MS.gene048705:CDS
GGGAGGTAAGGGACTTGGAA+AGG	0.449159	3.2:-60976422	MS.gene048705:CDS
GGAGGTAAGGGACTTGGAAA+GGG	0.467307	3.2:-60976421	MS.gene048705:CDS
GGAAAGGGAGGTAAGGGACT+TGG	0.470633	3.2:-60976427	MS.gene048705:CDS
TTCAACCTCCAAATCCATAG+AGG	0.476286	3.2:+60976149	None:intergenic
CTGTTGAAAATGTCAGGTCG+TGG	0.487154	3.2:-60976448	None:intergenic
CAATCGACGAATTGCAGGCT+TGG	0.491738	3.2:+60976348	None:intergenic
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.2:-60976434	MS.gene048705:CDS
AGTTCTCCGCGATAACATCC+AGG	0.504812	3.2:-60976377	MS.gene048705:CDS
AGAACCCTCTATGGATTTGG+AGG	0.508817	3.2:-60976154	MS.gene048705:CDS
GAAGATCTTCAAGACACCTC+TGG	0.510902	3.2:+60976276	None:intergenic
CAAGCCTGCAATTCGTCGAT+TGG	0.511374	3.2:-60976347	MS.gene048705:CDS
TTACATACACTGAGCATGCT+AGG	0.520723	3.2:-60976228	MS.gene048705:CDS
GAGGAAGACGGTGACAGCCA+TGG	0.546349	3.2:-60976206	MS.gene048705:CDS
AGGGAGGTGCAAAGAGGCAT+AGG	0.547891	3.2:-60976402	MS.gene048705:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.548959	3.2:-60976442	MS.gene048705:CDS
GGTAAGGGACTTGGAAAGGG+AGG	0.560743	3.2:-60976418	MS.gene048705:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	3.2:-60976433	MS.gene048705:CDS
GTCTATGCTTTGAAGAGACA+AGG	0.570048	3.2:-60976178	MS.gene048705:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.2:-60976443	None:intergenic
CGTCGATTGGCGAGAAGAGG+CGG	0.593362	3.2:-60976334	MS.gene048705:CDS
GGCGGTGTTAAGAGAATCAG+TGG	0.595979	3.2:-60976316	MS.gene048705:CDS
ATTCGTCGATTGGCGAGAAG+AGG	0.604517	3.2:-60976337	MS.gene048705:CDS
TTGCAGGCTTGGTGATACCC+TGG	0.613296	3.2:+60976359	None:intergenic
CAAAGCATAGACAACATCCA+TGG	0.629842	3.2:+60976189	None:intergenic
TCAACCTCCAAATCCATAGA+GGG	0.633476	3.2:+60976150	None:intergenic
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.2:-60976439	MS.gene048705:CDS
TGATACCCTGGATGTTATCG+CGG	0.665173	3.2:+60976371	None:intergenic
TGAGCATGCTAGGAGGAAGA+CGG	0.671956	3.2:-60976218	MS.gene048705:CDS
GTTCTCCGCGATAACATCCA+GGG	0.672360	3.2:-60976376	MS.gene048705:CDS
TTGGAAAGGGAGGTGCAAAG+AGG	0.683014	3.2:-60976408	MS.gene048705:CDS
TTGATCTATGAAGAAACCAG+AGG	0.704889	3.2:-60976292	MS.gene048705:CDS
CATACACTGAGCATGCTAGG+AGG	0.707859	3.2:-60976225	MS.gene048705:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATCTATGAAGAAACCAG+AGG	-	chr3.2:60976297-60976316	MS.gene048705:CDS	35.0%
	AGGTGTCTTGAAGATCTTCT+TGG	-	chr3.2:60976317-60976336	MS.gene048705:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	+	chr3.2:60976403-60976422	None:intergenic	40.0%
	TCAACCTCCAAATCCATAGA+GGG	+	chr3.2:60976442-60976461	None:intergenic	40.0%
	TTACATACACTGAGCATGCT+AGG	-	chr3.2:60976361-60976380	MS.gene048705:CDS	40.0%
!!	GTCTATGCTTTGAAGAGACA+AGG	-	chr3.2:60976411-60976430	MS.gene048705:CDS	40.0%
	AGAACCCTCTATGGATTTGG+AGG	-	chr3.2:60976435-60976454	MS.gene048705:CDS	45.0%
	AGACAAGGAAGAACCCTCTA+TGG	-	chr3.2:60976426-60976445	MS.gene048705:CDS	45.0%
	GAAGATCTTCAAGACACCTC+TGG	+	chr3.2:60976316-60976335	None:intergenic	45.0%
	GGAAGAACCCTCTATGGATT+TGG	-	chr3.2:60976432-60976451	MS.gene048705:CDS	45.0%
	TGATACCCTGGATGTTATCG+CGG	+	chr3.2:60976221-60976240	None:intergenic	45.0%
	AGTTCTCCGCGATAACATCC+AGG	-	chr3.2:60976212-60976231	MS.gene048705:CDS	50.0%
	CAAGCCTGCAATTCGTCGAT+TGG	-	chr3.2:60976242-60976261	MS.gene048705:CDS	50.0%
	CAATCGACGAATTGCAGGCT+TGG	+	chr3.2:60976244-60976263	None:intergenic	50.0%
	CATACACTGAGCATGCTAGG+AGG	-	chr3.2:60976364-60976383	MS.gene048705:CDS	50.0%
	GGAGGTAAGGGACTTGGAAA+GGG	-	chr3.2:60976168-60976187	MS.gene048705:CDS	50.0%
	GTTCTCCGCGATAACATCCA+GGG	-	chr3.2:60976213-60976232	MS.gene048705:CDS	50.0%
	TGAGCATGCTAGGAGGAAGA+CGG	-	chr3.2:60976371-60976390	MS.gene048705:CDS	50.0%
!	ATTCGTCGATTGGCGAGAAG+AGG	-	chr3.2:60976252-60976271	MS.gene048705:CDS	50.0%
!	GGCGGTGTTAAGAGAATCAG+TGG	-	chr3.2:60976273-60976292	MS.gene048705:CDS	50.0%
!	TTGGAAAGGGAGGTGCAAAG+AGG	-	chr3.2:60976181-60976200	MS.gene048705:CDS	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	-	chr3.2:60976155-60976174	MS.gene048705:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	-	chr3.2:60976150-60976169	MS.gene048705:CDS	55.0%
	CTCGCCAATCGACGAATTGC+AGG	+	chr3.2:60976249-60976268	None:intergenic	55.0%
	GGAAAGGGAGGTAAGGGACT+TGG	-	chr3.2:60976162-60976181	MS.gene048705:CDS	55.0%
	GGGAGGTAAGGGACTTGGAA+AGG	-	chr3.2:60976167-60976186	MS.gene048705:CDS	55.0%
	GGTAAGGGACTTGGAAAGGG+AGG	-	chr3.2:60976171-60976190	MS.gene048705:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	-	chr3.2:60976156-60976175	MS.gene048705:CDS	55.0%
!	AGGGAGGTGCAAAGAGGCAT+AGG	-	chr3.2:60976187-60976206	MS.gene048705:CDS	55.0%
!!	TTGCAGGCTTGGTGATACCC+TGG	+	chr3.2:60976233-60976252	None:intergenic	55.0%
	GAGGAAGACGGTGACAGCCA+TGG	-	chr3.2:60976383-60976402	MS.gene048705:CDS	60.0%
!	CGTCGATTGGCGAGAAGAGG+CGG	-	chr3.2:60976255-60976274	MS.gene048705:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	60976150	60976461	60976150	ID=MS.gene048705
chr3.2	mRNA	60976150	60976461	60976150	ID=MS.gene048705.t1;Parent=MS.gene048705
chr3.2	exon	60976150	60976461	60976150	ID=MS.gene048705.t1.exon1;Parent=MS.gene048705.t1
chr3.2	CDS	60976150	60976461	60976150	ID=cds.MS.gene048705.t1;Parent=MS.gene048705.t1

Gene Sequence	>MS.gene048705 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTTGGAAAGGGAGGTGCAAAGAGGCATAGGAAAGTTCTCCGCGATAACATCCAGGGTATCACCAAGCCTGCAATTCGTCGATTGGCGAGAAGAGGCGGTGTTAAGAGAATCAGTGGATTGATCTATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAACGTCATTCGTGATGCAGTTACATACACTGAGCATGCTAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAAGGAAGAACCCTCTATGGATTTGGAGGTTGA
Protein sequence	>MS.gene048705.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG