AlfalfaGEDB | MS.gene035721

M. sativa cultivar XinJiangDaYe / MS.gene035721

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035721.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035721.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035721.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene009027	MS.gene035721	PPI
MS.gene31196	MS.gene035721	PPI
MS.gene035721	MS.gene049705	PPI
MS.gene035721	MS.gene04434	PPI
MS.gene035721	MS.gene070907	PPI
MS.gene060465	MS.gene035721	PPI
MS.gene035721	MS.gene04392	PPI
MS.gene035721	MS.gene010219	PPI
MS.gene035721	MS.gene05608	PPI
MS.gene035721	MS.gene68545	PPI
MS.gene035721	MS.gene070242	PPI
MS.gene035721	MS.gene82546	PPI
MS.gene08121	MS.gene035721	PPI
MS.gene035721	MS.gene048746	PPI
MS.gene061870	MS.gene035721	PPI
MS.gene035721	MS.gene09873	PPI
MS.gene035718	MS.gene035721	PPI
MS.gene035721	MS.gene043967	PPI
MS.gene035721	MS.gene62520	PPI
MS.gene035721	MS.gene035897	PPI
MS.gene78578	MS.gene035721	PPI
MS.gene043967	MS.gene035721	PPI
MS.gene064557	MS.gene035721	PPI
MS.gene028629	MS.gene035721	PPI
MS.gene035721	MS.gene072493	PPI
MS.gene04433	MS.gene035721	PPI
MS.gene04452	MS.gene035721	PPI
MS.gene035721	MS.gene84536	PPI
MS.gene035721	MS.gene035896	PPI
MS.gene035721	MS.gene061866	PPI
MS.gene035787	MS.gene035721	PPI
MS.gene04067	MS.gene035721	PPI
MS.gene71215	MS.gene035721	PPI
MS.gene035721	MS.gene64650	PPI
MS.gene035721	MS.gene60093	PPI
MS.gene89487	MS.gene035721	PPI
MS.gene035721	MS.gene08122	PPI
MS.gene035721	MS.gene061869	PPI
MS.gene035719	MS.gene035721	PPI
MS.gene035721	MS.gene022932	PPI
MS.gene58854	MS.gene035721	PPI
MS.gene66225	MS.gene035721	PPI
MS.gene70955	MS.gene035721	PPI
MS.gene071136	MS.gene035721	PPI
MS.gene035721	MS.gene010220	PPI
MS.gene040383	MS.gene035721	PPI
MS.gene070242	MS.gene035721	PPI
MS.gene035721	MS.gene04972	PPI
MS.gene035721	MS.gene004121	PPI
MS.gene010220	MS.gene035721	PPI
MS.gene035721	MS.gene018578	PPI
MS.gene035721	MS.gene061867	PPI
MS.gene88680	MS.gene035721	PPI
MS.gene035721	MS.gene04064	PPI
MS.gene035721	MS.gene018580	PPI
MS.gene056215	MS.gene035721	PPI
MS.gene035721	MS.gene004124	PPI
MS.gene038469	MS.gene035721	PPI
MS.gene035721	MS.gene05619	PPI
MS.gene061876	MS.gene035721	PPI
MS.gene035721	MS.gene04052	PPI
MS.gene035721	MS.gene89488	PPI
MS.gene035721	MS.gene66225	PPI
MS.gene035721	MS.gene79195	PPI
MS.gene035721	MS.gene048705	PPI
MS.gene035721	MS.gene61017	PPI
MS.gene060463	MS.gene035721	PPI
MS.gene035721	MS.gene061874	PPI
MS.gene035721	MS.gene04576	PPI
MS.gene035721	MS.gene012390	PPI
MS.gene30920	MS.gene035721	PPI
MS.gene08053	MS.gene035721	PPI
MS.gene061872	MS.gene035721	PPI
MS.gene070241	MS.gene035721	PPI
MS.gene035721	MS.gene016624	PPI
MS.gene035721	MS.gene008276	PPI
MS.gene035721	MS.gene73136	PPI
MS.gene035721	MS.gene049821	PPI
MS.gene035721	MS.gene04068	PPI
MS.gene035721	MS.gene022946	PPI
MS.gene022932	MS.gene035721	PPI
MS.gene035721	MS.gene05623	PPI
MS.gene012391	MS.gene035721	PPI
MS.gene88681	MS.gene035721	PPI
MS.gene070244	MS.gene035721	PPI
MS.gene035896	MS.gene035721	PPI
MS.gene64920	MS.gene035721	PPI
MS.gene035721	MS.gene035720	PPI
MS.gene035721	MS.gene09871	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035721.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035721.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035721.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035721.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035721.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035721.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035721.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035721.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035721.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035721.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035721.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035721.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035721.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035721.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene035721.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene035721.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035721.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035721.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035721.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035721.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035721.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035721.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035721.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035721.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035721.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035721.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene035721.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 32 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGCAGGGAAGGACTCTTTA+TGG	0.247348	8.4:-6176921	MS.gene035721:CDS
GGAAGGACTCTTTATGGATT+TGG	0.297351	8.4:-6176915	MS.gene035721:CDS
TCCTTTGCCTCTTCCTGACA+TGG	0.418595	8.4:+6177199	None:intergenic
TCTTTATGGATTTGGCGGTT+AGG	0.439485	8.4:-6176907	None:intergenic
GGCAAAGGAGGAAAGGGTCT+TGG	0.446329	8.4:-6177185	MS.gene035721:CDS
CAAGAATTCACAGCCATGTC+AGG	0.481900	8.4:-6177212	None:intergenic
CGTTTACGCTCTCAAGAGGC+AGG	0.488613	8.4:-6176937	MS.gene035721:CDS
GGTTCTTCGCGATAACATCC+AGG	0.500175	8.4:-6177135	MS.gene035721:CDS
AGGACTCTTTATGGATTTGG+CGG	0.509079	8.4:-6176912	MS.gene035721:CDS
GGAGGAAAGGGTCTTGGAAA+GGG	0.511441	8.4:-6177179	MS.gene035721:CDS
TCGCAGGCTTCGTGATTCCC+TGG	0.511861	8.4:+6177117	None:intergenic
AGGAAGAGGCAAAGGAGGAA+AGG	0.518412	8.4:-6177192	MS.gene035721:CDS
GGAAGAGGCAAAGGAGGAAA+GGG	0.527098	8.4:-6177191	MS.gene035721:CDS
CCATCGCAGTAACAGTCTTA+CGG	0.545551	8.4:+6176964	None:intergenic
GCCATGTCAGGAAGAGGCAA+AGG	0.547501	8.4:-6177200	MS.gene035721:CDS
AGGAGGAAAGGGTCTTGGAA+AGG	0.553664	8.4:-6177180	MS.gene035721:CDS
CCGTAAGACTGTTACTGCGA+TGG	0.561095	8.4:-6176964	MS.gene035721:CDS
CTTGCGAGACGACGAATCGC+AGG	0.571080	8.4:+6177101	None:intergenic
TTCACAGCCATGTCAGGAAG+AGG	0.573281	8.4:-6177206	None:intergenic
AGGAGCAAAACGACACCGTA+AGG	0.575994	8.4:-6177156	MS.gene035721:CDS
TGTTATCGCGAAGAACCTTA+CGG	0.576355	8.4:+6177141	None:intergenic
ATGTCAGGAAGAGGCAAAGG+AGG	0.581057	8.4:-6177197	MS.gene035721:CDS
ACGCTCTCAAGAGGCAGGGA+AGG	0.583359	8.4:-6176932	MS.gene035721:CDS
ACGGCGAGCATGCTCTGTGT+AGG	0.590446	8.4:+6176983	None:intergenic
GGAAAGGGTCTTGGAAAGGG+AGG	0.621886	8.4:-6177176	MS.gene035721:CDS
ATTCGTCGTCTCGCAAGACG+TGG	0.624267	8.4:-6177095	MS.gene035721:CDS
TTAATCTATGAAGAAACTCG+TGG	0.634992	8.4:-6177050	MS.gene035721:CDS
ATGTCGTTTACGCTCTCAAG+AGG	0.636714	8.4:-6176941	MS.gene035721:CDS
CGTCGTCTCGCAAGACGTGG+TGG	0.642630	8.4:-6177092	MS.gene035721:CDS
GTTCTTCGCGATAACATCCA+GGG	0.657095	8.4:-6177134	MS.gene035721:CDS
GTTTACGCTCTCAAGAGGCA+GGG	0.663401	8.4:-6176936	MS.gene035721:CDS
GGTGGTGTGAAGCGTATCAG+TGG	0.669421	8.4:-6177074	MS.gene035721:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTAATCTATGAAGAAACTCG+TGG	-	chr8.4:6177055-6177074	MS.gene035721:CDS	30.0%
	AGGACTCTTTATGGATTTGG+CGG	-	chr8.4:6177193-6177212	MS.gene035721:CDS	40.0%
	GGAAGGACTCTTTATGGATT+TGG	-	chr8.4:6177190-6177209	MS.gene035721:CDS	40.0%
	TGTTATCGCGAAGAACCTTA+CGG	+	chr8.4:6176967-6176986	None:intergenic	40.0%
	CCATCGCAGTAACAGTCTTA+CGG	+	chr8.4:6177144-6177163	None:intergenic	45.0%
	GTTCTTCGCGATAACATCCA+GGG	-	chr8.4:6176971-6176990	MS.gene035721:CDS	45.0%
!	ATGTCGTTTACGCTCTCAAG+AGG	-	chr8.4:6177164-6177183	MS.gene035721:CDS	45.0%
	AGGAAGAGGCAAAGGAGGAA+AGG	-	chr8.4:6176913-6176932	MS.gene035721:CDS	50.0%
	AGGAGCAAAACGACACCGTA+AGG	-	chr8.4:6176949-6176968	MS.gene035721:CDS	50.0%
	AGGAGGAAAGGGTCTTGGAA+AGG	-	chr8.4:6176925-6176944	MS.gene035721:CDS	50.0%
	AGGCAGGGAAGGACTCTTTA+TGG	-	chr8.4:6177184-6177203	MS.gene035721:CDS	50.0%
	ATGTCAGGAAGAGGCAAAGG+AGG	-	chr8.4:6176908-6176927	MS.gene035721:CDS	50.0%
	CCGTAAGACTGTTACTGCGA+TGG	-	chr8.4:6177141-6177160	MS.gene035721:CDS	50.0%
	GGAAGAGGCAAAGGAGGAAA+GGG	-	chr8.4:6176914-6176933	MS.gene035721:CDS	50.0%
	GGAGGAAAGGGTCTTGGAAA+GGG	-	chr8.4:6176926-6176945	MS.gene035721:CDS	50.0%
	GGTTCTTCGCGATAACATCC+AGG	-	chr8.4:6176970-6176989	MS.gene035721:CDS	50.0%
	GTTTACGCTCTCAAGAGGCA+GGG	-	chr8.4:6177169-6177188	MS.gene035721:CDS	50.0%
	ATTCGTCGTCTCGCAAGACG+TGG	-	chr8.4:6177010-6177029	MS.gene035721:CDS	55.0%
	CGTTTACGCTCTCAAGAGGC+AGG	-	chr8.4:6177168-6177187	MS.gene035721:CDS	55.0%
	GGAAAGGGTCTTGGAAAGGG+AGG	-	chr8.4:6176929-6176948	MS.gene035721:CDS	55.0%
	GGCAAAGGAGGAAAGGGTCT+TGG	-	chr8.4:6176920-6176939	MS.gene035721:CDS	55.0%
	GGTGGTGTGAAGCGTATCAG+TGG	-	chr8.4:6177031-6177050	MS.gene035721:CDS	55.0%
	ACGCTCTCAAGAGGCAGGGA+AGG	-	chr8.4:6177173-6177192	MS.gene035721:CDS	60.0%
	ACGGCGAGCATGCTCTGTGT+AGG	+	chr8.4:6177125-6177144	None:intergenic	60.0%
	CTTGCGAGACGACGAATCGC+AGG	+	chr8.4:6177007-6177026	None:intergenic	60.0%
	TCGCAGGCTTCGTGATTCCC+TGG	+	chr8.4:6176991-6177010	None:intergenic	60.0%
	CGTCGTCTCGCAAGACGTGG+TGG	-	chr8.4:6177013-6177032	MS.gene035721:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	6176908	6177219	6176908	ID=MS.gene035721
chr8.4	mRNA	6176908	6177219	6176908	ID=MS.gene035721.t1;Parent=MS.gene035721
chr8.4	exon	6176908	6177219	6176908	ID=MS.gene035721.t1.exon1;Parent=MS.gene035721.t1
chr8.4	CDS	6176908	6177219	6176908	ID=cds.MS.gene035721.t1;Parent=MS.gene035721.t1

Gene Sequence	>MS.gene035721 ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTCTTGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTTCGCGATAACATCCAGGGAATCACGAAGCCTGCGATTCGTCGTCTCGCAAGACGTGGTGGTGTGAAGCGTATCAGTGGCTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAATGTGATTCGTGATGCTGTCACCTACACAGAGCATGCTCGCCGTAAGACTGTTACTGCGATGGATGTCGTTTACGCTCTCAAGAGGCAGGGAAGGACTCTTTATGGATTTGGCGGTTAG
Protein sequence	>MS.gene035721.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG