AlfalfaGEDB | MS.gene08053

M. sativa cultivar XinJiangDaYe / MS.gene08053

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08053.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08053.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08053.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene31196	MS.gene08053	PPI
MS.gene08053	MS.gene04452	PPI
MS.gene060465	MS.gene08053	PPI
MS.gene08053	MS.gene061867	PPI
MS.gene08053	MS.gene008276	PPI
MS.gene08053	MS.gene05619	PPI
MS.gene08053	MS.gene73136	PPI
MS.gene08053	MS.gene049821	PPI
MS.gene08053	MS.gene04068	PPI
MS.gene061870	MS.gene08053	PPI
MS.gene08053	MS.gene022946	PPI
MS.gene08053	MS.gene05623	PPI
MS.gene08053	MS.gene89488	PPI
MS.gene035718	MS.gene08053	PPI
MS.gene08053	MS.gene66225	PPI
MS.gene08053	MS.gene035720	PPI
MS.gene08053	MS.gene70955	PPI
MS.gene08053	MS.gene61017	PPI
MS.gene08053	MS.gene09871	PPI
MS.gene071136	MS.gene08053	PPI
MS.gene08053	MS.gene012390	PPI
MS.gene08053	MS.gene08121	PPI
MS.gene08053	MS.gene64920	PPI
MS.gene08053	MS.gene064557	PPI
MS.gene78578	MS.gene08053	PPI
MS.gene08053	MS.gene04434	PPI
MS.gene08053	MS.gene070907	PPI
MS.gene08053	MS.gene04392	PPI
MS.gene08053	MS.gene010219	PPI
MS.gene08053	MS.gene040383	PPI
MS.gene038469	MS.gene08053	PPI
MS.gene08053	MS.gene68545	PPI
MS.gene028629	MS.gene08053	PPI
MS.gene08053	MS.gene070242	PPI
MS.gene08053	MS.gene016624	PPI
MS.gene04433	MS.gene08053	PPI
MS.gene035787	MS.gene08053	PPI
MS.gene04067	MS.gene08053	PPI
MS.gene71215	MS.gene08053	PPI
MS.gene08053	MS.gene048746	PPI
MS.gene89487	MS.gene08053	PPI
MS.gene035719	MS.gene08053	PPI
MS.gene58854	MS.gene08053	PPI
MS.gene08053	MS.gene043967	PPI
MS.gene061872	MS.gene08053	PPI
MS.gene08053	MS.gene035897	PPI
MS.gene08053	MS.gene049705	PPI
MS.gene88680	MS.gene08053	PPI
MS.gene056215	MS.gene08053	PPI
MS.gene08053	MS.gene05608	PPI
MS.gene061876	MS.gene08053	PPI
MS.gene08053	MS.gene82546	PPI
MS.gene08053	MS.gene60093	PPI
MS.gene08053	MS.gene08122	PPI
MS.gene060463	MS.gene08053	PPI
MS.gene08053	MS.gene09873	PPI
MS.gene08053	MS.gene022932	PPI
MS.gene08053	MS.gene010220	PPI
MS.gene08053	MS.gene04972	PPI
MS.gene08053	MS.gene004121	PPI
MS.gene30920	MS.gene08053	PPI
MS.gene08053	MS.gene62520	PPI
MS.gene08053	MS.gene018578	PPI
MS.gene08053	MS.gene035721	PPI
MS.gene08053	MS.gene04064	PPI
MS.gene08053	MS.gene018580	PPI
MS.gene070241	MS.gene08053	PPI
MS.gene08053	MS.gene004124	PPI
MS.gene08053	MS.gene072493	PPI
MS.gene08053	MS.gene04052	PPI
MS.gene08053	MS.gene84536	PPI
MS.gene08053	MS.gene035896	PPI
MS.gene08053	MS.gene061866	PPI
MS.gene012391	MS.gene08053	PPI
MS.gene88681	MS.gene08053	PPI
MS.gene070244	MS.gene08053	PPI
MS.gene08053	MS.gene64650	PPI
MS.gene64920	MS.gene08053	PPI
MS.gene08053	MS.gene79195	PPI
MS.gene08053	MS.gene048705	PPI
MS.gene08053	MS.gene061874	PPI
MS.gene08053	MS.gene04576	PPI
MS.gene08053	MS.gene061869	PPI
MS.gene009027	MS.gene08053	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08053.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08053.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08053.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08053.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08053.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08053.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08053.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08053.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08053.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08053.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08053.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08053.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08053.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08053.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene08053.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene08053.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08053.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08053.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08053.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08053.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08053.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08053.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08053.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08053.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08053.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08053.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene08053.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 38 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGCCAATCGACGAATTGC+AGG	0.295956	3.3:-49053490	None:intergenic
GGAAGAACCCTCTATGGATT+TGG	0.370047	3.3:+49053676	MS.gene08053:CDS
AGACAAGGAAGAACCCTCTA+TGG	0.396310	3.3:+49053670	MS.gene08053:CDS
AGGTGTCTTGAAGATCTTCT+TGG	0.406404	3.3:+49053561	MS.gene08053:CDS
GGAAAGGGAGGTAAGGGATT+GGG	0.427677	3.3:+49053406	MS.gene08053:CDS
CTCTTGAAAATGTCAGGTCG+TGG	0.456977	3.3:+49053385	None:intergenic
GGAGGTAAGGGATTGGGAAA+GGG	0.467307	3.3:+49053412	MS.gene08053:CDS
GGGAGGTAAGGGATTGGGAA+AGG	0.474781	3.3:+49053411	MS.gene08053:CDS
TTCAACCTCCAAATCCATAG+AGG	0.476286	3.3:-49053684	None:intergenic
CAATCGACGAATTGCAGGCT+TGG	0.486859	3.3:-49053485	None:intergenic
GGTAAGGGATTGGGAAAGGG+AGG	0.489050	3.3:+49053415	MS.gene08053:CDS
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.3:+49053399	MS.gene08053:CDS
AGGGAGGTGCAAAGAGGCAT+AGG	0.498985	3.3:+49053431	MS.gene08053:CDS
AGAACCCTCTATGGATTTGG+AGG	0.508817	3.3:+49053679	MS.gene08053:CDS
CAAGCCTGCAATTCGTCGAT+TGG	0.509017	3.3:+49053486	MS.gene08053:CDS
GAAGATCTTCAAGACACCTC+TGG	0.510902	3.3:-49053557	None:intergenic
TGGAAAGGGAGGTAAGGGAT+TGG	0.528271	3.3:+49053405	MS.gene08053:CDS
TTACATACACTGAGCATGCT+AGG	0.534439	3.3:+49053605	MS.gene08053:CDS
GGTTCTTCGTGATAACATCC+AGG	0.535931	3.3:+49053456	MS.gene08053:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.538992	3.3:+49053391	None:intergenic
GGTGGTGTTAAGAGAATCAG+TGG	0.556350	3.3:+49053517	MS.gene08053:CDS
GTCTATGCTTTGAAGAGACA+AGG	0.570048	3.3:+49053655	MS.gene08053:CDS
GAGGAAGACTGTGACAGCCA+TGG	0.570644	3.3:+49053627	MS.gene08053:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.574068	3.3:+49053400	MS.gene08053:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.3:+49053390	None:intergenic
AGGTGCAAAGAGGCATAGGA+AGG	0.586086	3.3:+49053435	MS.gene08053:CDS
CGTCGATTGGCAAGAAGAGG+TGG	0.589209	3.3:+49053499	MS.gene08053:CDS
CAGTGTATGTAACGGCATCA+CGG	0.612887	3.3:-49053594	None:intergenic
TTGCAGGCTTGGTGATACCC+TGG	0.613296	3.3:-49053474	None:intergenic
ATTCGTCGATTGGCAAGAAG+AGG	0.624691	3.3:+49053496	MS.gene08053:CDS
CAAAGCATAGACAACATCCA+TGG	0.629842	3.3:-49053644	None:intergenic
TCAACCTCCAAATCCATAGA+GGG	0.633476	3.3:-49053683	None:intergenic
AGCATGCTCAGTGTATGTAA+CGG	0.638626	3.3:-49053602	None:intergenic
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.3:+49053394	MS.gene08053:CDS
GTTCTTCGTGATAACATCCA+GGG	0.651580	3.3:+49053457	MS.gene08053:CDS
TGGGAAAGGGAGGTGCAAAG+AGG	0.673404	3.3:+49053425	MS.gene08053:CDS
TTGATCTATGAAGAAACCAG+AGG	0.704889	3.3:+49053541	MS.gene08053:CDS
CATACACTGAGCATGCTAGG+AGG	0.707859	3.3:+49053608	MS.gene08053:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATCTATGAAGAAACCAG+AGG	+	chr3.3:49053541-49053560	MS.gene08053:CDS	35.0%
	AGCATGCTCAGTGTATGTAA+CGG	-	chr3.3:49053605-49053624	None:intergenic	40.0%
	AGGTGTCTTGAAGATCTTCT+TGG	+	chr3.3:49053561-49053580	MS.gene08053:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	-	chr3.3:49053647-49053666	None:intergenic	40.0%
	GTTCTTCGTGATAACATCCA+GGG	+	chr3.3:49053457-49053476	MS.gene08053:CDS	40.0%
	TCAACCTCCAAATCCATAGA+GGG	-	chr3.3:49053686-49053705	None:intergenic	40.0%
	TTACATACACTGAGCATGCT+AGG	+	chr3.3:49053605-49053624	MS.gene08053:CDS	40.0%
!!	GTCTATGCTTTGAAGAGACA+AGG	+	chr3.3:49053655-49053674	MS.gene08053:CDS	40.0%
	AGAACCCTCTATGGATTTGG+AGG	+	chr3.3:49053679-49053698	MS.gene08053:CDS	45.0%
	AGACAAGGAAGAACCCTCTA+TGG	+	chr3.3:49053670-49053689	MS.gene08053:CDS	45.0%
	CAGTGTATGTAACGGCATCA+CGG	-	chr3.3:49053597-49053616	None:intergenic	45.0%
	GAAGATCTTCAAGACACCTC+TGG	-	chr3.3:49053560-49053579	None:intergenic	45.0%
	GGAAGAACCCTCTATGGATT+TGG	+	chr3.3:49053676-49053695	MS.gene08053:CDS	45.0%
	GGTTCTTCGTGATAACATCC+AGG	+	chr3.3:49053456-49053475	MS.gene08053:CDS	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	+	chr3.3:49053496-49053515	MS.gene08053:CDS	45.0%
!	GGTGGTGTTAAGAGAATCAG+TGG	+	chr3.3:49053517-49053536	MS.gene08053:CDS	45.0%
	CAAGCCTGCAATTCGTCGAT+TGG	+	chr3.3:49053486-49053505	MS.gene08053:CDS	50.0%
	CAATCGACGAATTGCAGGCT+TGG	-	chr3.3:49053488-49053507	None:intergenic	50.0%
	CATACACTGAGCATGCTAGG+AGG	+	chr3.3:49053608-49053627	MS.gene08053:CDS	50.0%
	CTTGCCAATCGACGAATTGC+AGG	-	chr3.3:49053493-49053512	None:intergenic	50.0%
	GGAAAGGGAGGTAAGGGATT+GGG	+	chr3.3:49053406-49053425	MS.gene08053:CDS	50.0%
	GGAGGTAAGGGATTGGGAAA+GGG	+	chr3.3:49053412-49053431	MS.gene08053:CDS	50.0%
	TGGAAAGGGAGGTAAGGGAT+TGG	+	chr3.3:49053405-49053424	MS.gene08053:CDS	50.0%
!	AGGTGCAAAGAGGCATAGGA+AGG	+	chr3.3:49053435-49053454	MS.gene08053:CDS	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	+	chr3.3:49053399-49053418	MS.gene08053:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	+	chr3.3:49053394-49053413	MS.gene08053:CDS	55.0%
	GAGGAAGACTGTGACAGCCA+TGG	+	chr3.3:49053627-49053646	MS.gene08053:CDS	55.0%
	GGGAGGTAAGGGATTGGGAA+AGG	+	chr3.3:49053411-49053430	MS.gene08053:CDS	55.0%
	GGTAAGGGATTGGGAAAGGG+AGG	+	chr3.3:49053415-49053434	MS.gene08053:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	+	chr3.3:49053400-49053419	MS.gene08053:CDS	55.0%
!	AGGGAGGTGCAAAGAGGCAT+AGG	+	chr3.3:49053431-49053450	MS.gene08053:CDS	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	+	chr3.3:49053499-49053518	MS.gene08053:CDS	55.0%
!	TGGGAAAGGGAGGTGCAAAG+AGG	+	chr3.3:49053425-49053444	MS.gene08053:CDS	55.0%
!!	TTGCAGGCTTGGTGATACCC+TGG	-	chr3.3:49053477-49053496	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	49053394	49053705	49053394	ID=MS.gene08053
chr3.3	mRNA	49053394	49053705	49053394	ID=MS.gene08053.t1;Parent=MS.gene08053
chr3.3	exon	49053394	49053705	49053394	ID=MS.gene08053.t1.exon1;Parent=MS.gene08053.t1
chr3.3	CDS	49053394	49053705	49053394	ID=cds.MS.gene08053.t1;Parent=MS.gene08053.t1

Gene Sequence	>MS.gene08053 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGATTGGGAAAGGGAGGTGCAAAGAGGCATAGGAAGGTTCTTCGTGATAACATCCAGGGTATCACCAAGCCTGCAATTCGTCGATTGGCAAGAAGAGGTGGTGTTAAGAGAATCAGTGGATTGATCTATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAGAATGTCATCCGTGATGCCGTTACATACACTGAGCATGCTAGGAGGAAGACTGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAAGGAAGAACCCTCTATGGATTTGGAGGTTGA
Protein sequence	>MS.gene08053.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG