AlfalfaGEDB | MS.gene043967

M. sativa cultivar XinJiangDaYe / MS.gene043967

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043967.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043967.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043967.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene060465	MS.gene043967	PPI
MS.gene028629	MS.gene043967	PPI
MS.gene043967	MS.gene035897	PPI
MS.gene043967	MS.gene049705	PPI
MS.gene04067	MS.gene043967	PPI
MS.gene71215	MS.gene043967	PPI
MS.gene043967	MS.gene05608	PPI
MS.gene061870	MS.gene043967	PPI
MS.gene035718	MS.gene043967	PPI
MS.gene043967	MS.gene035896	PPI
MS.gene043967	MS.gene82546	PPI
MS.gene66225	MS.gene043967	PPI
MS.gene071136	MS.gene043967	PPI
MS.gene043967	MS.gene60093	PPI
MS.gene043967	MS.gene08122	PPI
MS.gene043967	MS.gene09873	PPI
MS.gene78578	MS.gene043967	PPI
MS.gene035721	MS.gene043967	PPI
MS.gene043967	MS.gene022932	PPI
MS.gene043967	MS.gene010220	PPI
MS.gene064557	MS.gene043967	PPI
MS.gene038469	MS.gene043967	PPI
MS.gene043967	MS.gene04972	PPI
MS.gene043967	MS.gene004121	PPI
MS.gene043967	MS.gene62520	PPI
MS.gene043967	MS.gene018578	PPI
MS.gene04433	MS.gene043967	PPI
MS.gene043967	MS.gene035721	PPI
MS.gene04452	MS.gene043967	PPI
MS.gene043967	MS.gene04064	PPI
MS.gene043967	MS.gene018580	PPI
MS.gene043967	MS.gene004124	PPI
MS.gene035787	MS.gene043967	PPI
MS.gene89487	MS.gene043967	PPI
MS.gene043967	MS.gene072493	PPI
MS.gene035719	MS.gene043967	PPI
MS.gene043967	MS.gene04052	PPI
MS.gene043967	MS.gene84536	PPI
MS.gene58854	MS.gene043967	PPI
MS.gene043967	MS.gene061866	PPI
MS.gene70955	MS.gene043967	PPI
MS.gene040383	MS.gene043967	PPI
MS.gene043967	MS.gene64650	PPI
MS.gene070242	MS.gene043967	PPI
MS.gene043967	MS.gene79195	PPI
MS.gene043967	MS.gene048705	PPI
MS.gene30920	MS.gene043967	PPI
MS.gene043967	MS.gene061874	PPI
MS.gene043967	MS.gene04576	PPI
MS.gene010220	MS.gene043967	PPI
MS.gene08053	MS.gene043967	PPI
MS.gene061872	MS.gene043967	PPI
MS.gene043967	MS.gene061869	PPI
MS.gene88680	MS.gene043967	PPI
MS.gene070241	MS.gene043967	PPI
MS.gene056215	MS.gene043967	PPI
MS.gene061876	MS.gene043967	PPI
MS.gene043967	MS.gene061867	PPI
MS.gene012391	MS.gene043967	PPI
MS.gene043967	MS.gene008276	PPI
MS.gene043967	MS.gene05619	PPI
MS.gene043967	MS.gene73136	PPI
MS.gene060463	MS.gene043967	PPI
MS.gene043967	MS.gene049821	PPI
MS.gene043967	MS.gene04068	PPI
MS.gene043967	MS.gene022946	PPI
MS.gene043967	MS.gene05623	PPI
MS.gene043967	MS.gene89488	PPI
MS.gene043967	MS.gene66225	PPI
MS.gene043967	MS.gene035720	PPI
MS.gene043967	MS.gene61017	PPI
MS.gene043967	MS.gene09871	PPI
MS.gene043967	MS.gene012390	PPI
MS.gene043967	MS.gene04434	PPI
MS.gene043967	MS.gene070907	PPI
MS.gene043967	MS.gene04392	PPI
MS.gene043967	MS.gene010219	PPI
MS.gene08121	MS.gene043967	PPI
MS.gene043967	MS.gene68545	PPI
MS.gene022932	MS.gene043967	PPI
MS.gene88681	MS.gene043967	PPI
MS.gene043967	MS.gene070242	PPI
MS.gene043967	MS.gene016624	PPI
MS.gene070244	MS.gene043967	PPI
MS.gene035896	MS.gene043967	PPI
MS.gene64920	MS.gene043967	PPI
MS.gene043967	MS.gene048746	PPI
MS.gene009027	MS.gene043967	PPI
MS.gene31196	MS.gene043967	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043967.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene043967.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene043967.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene043967.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene043967.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene043967.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene043967.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene043967.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene043967.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene043967.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene043967.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene043967.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene043967.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene043967.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene043967.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene043967.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043967.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene043967.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene043967.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene043967.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene043967.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene043967.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene043967.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene043967.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene043967.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene043967.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene043967.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 25 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAAGGACCCTTTATGGTTT+TGG	0.248657	5.3:-49809058	MS.gene043967:CDS
AGGCAAGGAAGGACCCTTTA+TGG	0.289623	5.3:-49809064	MS.gene043967:CDS
GGAAAAGGAGGAAAGGGTCT+TGG	0.394055	5.3:-49809328	MS.gene043967:CDS
GAGACGACGAATCGCGGGTT+TGG	0.422767	5.3:+49809249	None:intergenic
GTTCTTCGAGATAACATTCA+AGG	0.432889	5.3:-49809277	MS.gene043967:CDS
TCGCAAAACGGTGACTGCTA+TGG	0.458269	5.3:-49809107	MS.gene043967:CDS
GGAGGAAAGGGTCTTGGAAA+AGG	0.465706	5.3:-49809322	MS.gene043967:CDS
ATCTTGCAATCAACAATGTC+AGG	0.473641	5.3:-49809355	None:intergenic
ACAATGTCAGGAAGAGGAAA+AGG	0.493795	5.3:-49809343	MS.gene043967:CDS
GGAAGAGGAAAAGGAGGAAA+GGG	0.523720	5.3:-49809334	MS.gene043967:CDS
CCGCGATTCGTCGTCTCGCA+AGG	0.536114	5.3:-49809243	MS.gene043967:CDS
CAATCAACAATGTCAGGAAG+AGG	0.542220	5.3:-49809349	None:intergenic
CTTGCGAGACGACGAATCGC+GGG	0.552203	5.3:+49809244	None:intergenic
TGAACATGCTCGTCGCAAAA+CGG	0.556730	5.3:-49809119	MS.gene043967:CDS
AGGAAGAGGAAAAGGAGGAA+AGG	0.557567	5.3:-49809335	MS.gene043967:CDS
GTTTATGCGCTCAAGAGGCA+AGG	0.577445	5.3:-49809079	MS.gene043967:CDS
ATGCGCTCAAGAGGCAAGGA+AGG	0.588067	5.3:-49809075	MS.gene043967:CDS
CCTTGCGAGACGACGAATCG+CGG	0.599403	5.3:+49809243	None:intergenic
ATTCGTCGTCTCGCAAGGCG+TGG	0.610829	5.3:-49809238	MS.gene043967:CDS
ATGTTGTTTATGCGCTCAAG+AGG	0.611083	5.3:-49809084	MS.gene043967:CDS
ATGTCAGGAAGAGGAAAAGG+AGG	0.630961	5.3:-49809340	MS.gene043967:CDS
CGTCGTCTCGCAAGGCGTGG+TGG	0.632134	5.3:-49809235	MS.gene043967:CDS
GGAAAGGGTCTTGGAAAAGG+TGG	0.647254	5.3:-49809319	MS.gene043967:CDS
GGTGGTGTGAAGCGTATCAG+TGG	0.661880	5.3:-49809217	MS.gene043967:CDS
CTCATCTATGAAGAAACTCG+TGG	0.682677	5.3:-49809193	MS.gene043967:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GTTCTTCGAGATAACATTCA+AGG	-	chr5.3:49809114-49809133	MS.gene043967:CDS	35.0%
	CTAACCCCCAAAACCATAAA+GGG	+	chr5.3:49809343-49809362	None:intergenic	40.0%
	CTCATCTATGAAGAAACTCG+TGG	-	chr5.3:49809198-49809217	MS.gene043967:CDS	40.0%
!	ATGTTGTTTATGCGCTCAAG+AGG	-	chr5.3:49809307-49809326	MS.gene043967:CDS	40.0%
!	GAAGGACCCTTTATGGTTTT+GGG	-	chr5.3:49809334-49809353	MS.gene043967:CDS	40.0%
!!	AAGGACCCTTTATGGTTTTG+GGG	-	chr5.3:49809335-49809354	MS.gene043967:CDS	40.0%
	AGGAAGAGGAAAAGGAGGAA+AGG	-	chr5.3:49809056-49809075	MS.gene043967:CDS	45.0%
	ATGTCAGGAAGAGGAAAAGG+AGG	-	chr5.3:49809051-49809070	MS.gene043967:CDS	45.0%
	GGAAGAGGAAAAGGAGGAAA+GGG	-	chr5.3:49809057-49809076	MS.gene043967:CDS	45.0%
	TGAACATGCTCGTCGCAAAA+CGG	-	chr5.3:49809272-49809291	MS.gene043967:CDS	45.0%
!	GGAAGGACCCTTTATGGTTT+TGG	-	chr5.3:49809333-49809352	MS.gene043967:CDS	45.0%
!!!	AGGACCCTTTATGGTTTTGG+GGG	-	chr5.3:49809336-49809355	MS.gene043967:CDS	45.0%
	AGGCAAGGAAGGACCCTTTA+TGG	-	chr5.3:49809327-49809346	MS.gene043967:CDS	50.0%
	GGAAAAGGAGGAAAGGGTCT+TGG	-	chr5.3:49809063-49809082	MS.gene043967:CDS	50.0%
	GGAAAGGGTCTTGGAAAAGG+TGG	-	chr5.3:49809072-49809091	MS.gene043967:CDS	50.0%
	GGAGGAAAGGGTCTTGGAAA+AGG	-	chr5.3:49809069-49809088	MS.gene043967:CDS	50.0%
	GTTTATGCGCTCAAGAGGCA+AGG	-	chr5.3:49809312-49809331	MS.gene043967:CDS	50.0%
	TCGCAAAACGGTGACTGCTA+TGG	-	chr5.3:49809284-49809303	MS.gene043967:CDS	50.0%
	ATGCGCTCAAGAGGCAAGGA+AGG	-	chr5.3:49809316-49809335	MS.gene043967:CDS	55.0%
	GGTGGTGTGAAGCGTATCAG+TGG	-	chr5.3:49809174-49809193	MS.gene043967:CDS	55.0%
	ATTCGTCGTCTCGCAAGGCG+TGG	-	chr5.3:49809153-49809172	MS.gene043967:CDS	60.0%
	CCTTGCGAGACGACGAATCG+CGG	+	chr5.3:49809151-49809170	None:intergenic	60.0%
	CTTGCGAGACGACGAATCGC+GGG	+	chr5.3:49809150-49809169	None:intergenic	60.0%
	GAGACGACGAATCGCGGGTT+TGG	+	chr5.3:49809145-49809164	None:intergenic	60.0%
	CCGCGATTCGTCGTCTCGCA+AGG	-	chr5.3:49809148-49809167	MS.gene043967:CDS	65.0%
	CGTCGTCTCGCAAGGCGTGG+TGG	-	chr5.3:49809156-49809175	MS.gene043967:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	49809051	49809362	49809051	ID=MS.gene043967
chr5.3	mRNA	49809051	49809362	49809051	ID=MS.gene043967.t1;Parent=MS.gene043967
chr5.3	exon	49809051	49809362	49809051	ID=MS.gene043967.t1.exon1;Parent=MS.gene043967.t1
chr5.3	CDS	49809051	49809362	49809051	ID=cds.MS.gene043967.t1;Parent=MS.gene043967.t1

Gene Sequence	>MS.gene043967 ATGTCAGGAAGAGGAAAAGGAGGAAAGGGTCTTGGAAAAGGTGGTGCAAAGCGTCATCGCAAAGTTCTTCGAGATAACATTCAAGGTATCACCAAACCCGCGATTCGTCGTCTCGCAAGGCGTGGTGGTGTGAAGCGTATCAGTGGTCTCATCTATGAAGAAACTCGTGGTGTTCTCAAGATATTTCTTGAGAATGTTATTCGTGATGCTGTTACTTACACTGAACATGCTCGTCGCAAAACGGTGACTGCTATGGATGTTGTTTATGCGCTCAAGAGGCAAGGAAGGACCCTTTATGGTTTTGGGGGTTAG
Protein sequence	>MS.gene043967.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG