Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69886.t1 | XP_003601330.1 | 93.9 | 228 | 14 | 0 | 1 | 228 | 1 | 228 | 6.60E-113 | 416.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69886.t1 | P93236 | 28.9 | 149 | 98 | 4 | 56 | 198 | 505 | 651 | 7.9e-06 | 52.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69886.t1 | G7J637 | 93.9 | 228 | 14 | 0 | 1 | 228 | 1 | 228 | 4.8e-113 | 416.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene69886 | 0.825633 | 4.22E-54 | -1.69E-46 |
| MS.gene049217 | MS.gene69886 | 0.82347 | 1.36E-53 | -1.69E-46 |
| MS.gene049222 | MS.gene69886 | 0.817044 | 4.04E-52 | -1.69E-46 |
| MS.gene049285 | MS.gene69886 | 0.828114 | 1.08E-54 | -1.69E-46 |
| MS.gene049320 | MS.gene69886 | 0.846927 | 1.60E-59 | -1.69E-46 |
| MS.gene049351 | MS.gene69886 | 0.818511 | 1.89E-52 | -1.69E-46 |
| MS.gene049427 | MS.gene69886 | 0.877136 | 8.01E-69 | -1.69E-46 |
| MS.gene049609 | MS.gene69886 | 0.880523 | 5.12E-70 | -1.69E-46 |
| MS.gene049661 | MS.gene69886 | 0.870927 | 1.01E-66 | -1.69E-46 |
| MS.gene049662 | MS.gene69886 | 0.860015 | 2.77E-63 | -1.69E-46 |
| MS.gene049760 | MS.gene69886 | 0.812933 | 3.30E-51 | -1.69E-46 |
| MS.gene049805 | MS.gene69886 | 0.844756 | 6.21E-59 | -1.69E-46 |
| MS.gene050076 | MS.gene69886 | 0.833268 | 5.88E-56 | -1.69E-46 |
| MS.gene050163 | MS.gene69886 | 0.817823 | 2.70E-52 | -1.69E-46 |
| MS.gene050397 | MS.gene69886 | 0.854908 | 9.00E-62 | -1.69E-46 |
| MS.gene050432 | MS.gene69886 | 0.892691 | 1.26E-74 | -1.69E-46 |
| MS.gene050538 | MS.gene69886 | 0.810758 | 9.83E-51 | -1.69E-46 |
| MS.gene050593 | MS.gene69886 | 0.851083 | 1.12E-60 | -1.69E-46 |
| MS.gene050612 | MS.gene69886 | 0.803517 | 3.36E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene69886 | 0.804562 | 2.04E-49 | -1.69E-46 |
| MS.gene050675 | MS.gene69886 | 0.809319 | 2.01E-50 | -1.69E-46 |
| MS.gene050699 | MS.gene69886 | 0.833975 | 3.92E-56 | -1.69E-46 |
| MS.gene050747 | MS.gene69886 | 0.821473 | 3.96E-53 | -1.69E-46 |
| MS.gene050767 | MS.gene69886 | 0.847377 | 1.20E-59 | -1.69E-46 |
| MS.gene050770 | MS.gene69886 | 0.849695 | 2.74E-60 | -1.69E-46 |
| MS.gene050772 | MS.gene69886 | 0.876133 | 1.78E-68 | -1.69E-46 |
| MS.gene050950 | MS.gene69886 | 0.823686 | 1.21E-53 | -1.69E-46 |
| MS.gene05100 | MS.gene69886 | 0.809632 | 1.72E-50 | -1.69E-46 |
| MS.gene05101 | MS.gene69886 | 0.852585 | 4.19E-61 | -1.69E-46 |
| MS.gene05105 | MS.gene69886 | 0.859012 | 5.55E-63 | -1.69E-46 |
| MS.gene051228 | MS.gene69886 | 0.835682 | 1.46E-56 | -1.69E-46 |
| MS.gene051255 | MS.gene69886 | 0.806707 | 7.22E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene69886 | 0.809871 | 1.53E-50 | -1.69E-46 |
| MS.gene051551 | MS.gene69886 | 0.800401 | 1.47E-48 | -1.69E-46 |
| MS.gene051666 | MS.gene69886 | 0.861165 | 1.24E-63 | -1.69E-46 |
| MS.gene051692 | MS.gene69886 | 0.849424 | 3.26E-60 | -1.69E-46 |
| MS.gene051694 | MS.gene69886 | 0.813705 | 2.24E-51 | -1.69E-46 |
| MS.gene051810 | MS.gene69886 | 0.855846 | 4.80E-62 | -1.69E-46 |
| MS.gene051858 | MS.gene69886 | 0.85679 | 2.54E-62 | -1.69E-46 |
| MS.gene052078 | MS.gene69886 | 0.836131 | 1.12E-56 | -1.69E-46 |
| MS.gene052253 | MS.gene69886 | 0.80245 | 5.58E-49 | -1.69E-46 |
| MS.gene052271 | MS.gene69886 | 0.828805 | 7.33E-55 | -1.69E-46 |
| MS.gene052290 | MS.gene69886 | 0.822337 | 2.50E-53 | -1.69E-46 |
| MS.gene052310 | MS.gene69886 | 0.832479 | 9.24E-56 | -1.69E-46 |
| MS.gene052322 | MS.gene69886 | 0.858112 | 1.03E-62 | -1.69E-46 |
| MS.gene052379 | MS.gene69886 | 0.833802 | 4.33E-56 | -1.69E-46 |
| MS.gene052421 | MS.gene69886 | 0.81781 | 2.72E-52 | -1.69E-46 |
| MS.gene052481 | MS.gene69886 | 0.83843 | 2.89E-57 | -1.69E-46 |
| MS.gene052484 | MS.gene69886 | 0.836323 | 1.00E-56 | -1.69E-46 |
| MS.gene052501 | MS.gene69886 | 0.802082 | 6.64E-49 | -1.69E-46 |
| MS.gene052508 | MS.gene69886 | 0.837719 | 4.40E-57 | -1.69E-46 |
| MS.gene052510 | MS.gene69886 | 0.835291 | 1.83E-56 | -1.69E-46 |
| MS.gene052615 | MS.gene69886 | 0.850086 | 2.13E-60 | -1.69E-46 |
| MS.gene052870 | MS.gene69886 | 0.801383 | 9.24E-49 | -1.69E-46 |
| MS.gene052872 | MS.gene69886 | 0.80149 | 8.78E-49 | -1.69E-46 |
| MS.gene052916 | MS.gene69886 | 0.803874 | 2.83E-49 | -1.69E-46 |
| MS.gene052982 | MS.gene69886 | 0.817117 | 3.89E-52 | -1.69E-46 |
| MS.gene053009 | MS.gene69886 | 0.859093 | 5.25E-63 | -1.69E-46 |
| MS.gene053103 | MS.gene69886 | 0.829955 | 3.85E-55 | -1.69E-46 |
| MS.gene053104 | MS.gene69886 | 0.855838 | 4.82E-62 | -1.69E-46 |
| MS.gene053107 | MS.gene69886 | 0.817998 | 2.47E-52 | -1.69E-46 |
| MS.gene053207 | MS.gene69886 | 0.886711 | 2.70E-72 | -1.69E-46 |
| MS.gene053209 | MS.gene69886 | 0.877139 | 7.98E-69 | -1.69E-46 |
| MS.gene053213 | MS.gene69886 | 0.873648 | 1.25E-67 | -1.69E-46 |
| MS.gene053286 | MS.gene69886 | 0.864311 | 1.33E-64 | -1.69E-46 |
| MS.gene053322 | MS.gene69886 | 0.856128 | 3.97E-62 | -1.69E-46 |
| MS.gene053436 | MS.gene69886 | 0.808233 | 3.43E-50 | -1.69E-46 |
| MS.gene053449 | MS.gene69886 | 0.804515 | 2.08E-49 | -1.69E-46 |
| MS.gene053574 | MS.gene69886 | 0.847294 | 1.27E-59 | -1.69E-46 |
| MS.gene053632 | MS.gene69886 | 0.841771 | 3.88E-58 | -1.69E-46 |
| MS.gene053916 | MS.gene69886 | 0.80967 | 1.69E-50 | -1.69E-46 |
| MS.gene054376 | MS.gene69886 | 0.858688 | 6.94E-63 | -1.69E-46 |
| MS.gene054600 | MS.gene69886 | 0.848967 | 4.37E-60 | -1.69E-46 |
| MS.gene054642 | MS.gene69886 | 0.869496 | 2.96E-66 | -1.69E-46 |
| MS.gene054802 | MS.gene69886 | 0.834904 | 2.29E-56 | -1.69E-46 |
| MS.gene054803 | MS.gene69886 | 0.832417 | 9.57E-56 | -1.69E-46 |
| MS.gene054804 | MS.gene69886 | 0.838041 | 3.64E-57 | -1.69E-46 |
| MS.gene054927 | MS.gene69886 | 0.818475 | 1.92E-52 | -1.69E-46 |
| MS.gene05505 | MS.gene69886 | 0.841459 | 4.69E-58 | -1.69E-46 |
| MS.gene055116 | MS.gene69886 | 0.852962 | 3.27E-61 | -1.69E-46 |
| MS.gene055206 | MS.gene69886 | 0.84986 | 2.47E-60 | -1.69E-46 |
| MS.gene055212 | MS.gene69886 | 0.810306 | 1.23E-50 | -1.69E-46 |
| MS.gene055254 | MS.gene69886 | 0.856342 | 3.43E-62 | -1.69E-46 |
| MS.gene055277 | MS.gene69886 | 0.846319 | 2.34E-59 | -1.69E-46 |
| MS.gene055289 | MS.gene69886 | 0.85934 | 4.43E-63 | -1.69E-46 |
| MS.gene055362 | MS.gene69886 | 0.839388 | 1.63E-57 | -1.69E-46 |
| MS.gene055397 | MS.gene69886 | 0.862955 | 3.51E-64 | -1.69E-46 |
| MS.gene055499 | MS.gene69886 | 0.867537 | 1.27E-65 | -1.69E-46 |
| MS.gene055502 | MS.gene69886 | 0.803111 | 4.07E-49 | -1.69E-46 |
| MS.gene055577 | MS.gene69886 | 0.817676 | 2.91E-52 | -1.69E-46 |
| MS.gene055647 | MS.gene69886 | 0.841854 | 3.69E-58 | -1.69E-46 |
| MS.gene055722 | MS.gene69886 | 0.886753 | 2.60E-72 | -1.69E-46 |
| MS.gene05592 | MS.gene69886 | 0.873362 | 1.56E-67 | -1.69E-46 |
| MS.gene055941 | MS.gene69886 | 0.803498 | 3.39E-49 | -1.69E-46 |
| MS.gene056083 | MS.gene69886 | 0.86285 | 3.78E-64 | -1.69E-46 |
| MS.gene056191 | MS.gene69886 | 0.814263 | 1.68E-51 | -1.69E-46 |
| MS.gene056374 | MS.gene69886 | 0.871988 | 4.49E-67 | -1.69E-46 |
| MS.gene056407 | MS.gene69886 | 0.807454 | 5.01E-50 | -1.69E-46 |
| MS.gene056410 | MS.gene69886 | 0.82918 | 5.95E-55 | -1.69E-46 |
| MS.gene056487 | MS.gene69886 | 0.811971 | 5.36E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene69886 | 0.835275 | 1.84E-56 | -1.69E-46 |
| MS.gene056609 | MS.gene69886 | 0.850207 | 1.97E-60 | -1.69E-46 |
| MS.gene05664 | MS.gene69886 | 0.82184 | 3.26E-53 | -1.69E-46 |
| MS.gene056657 | MS.gene69886 | 0.845298 | 4.43E-59 | -1.69E-46 |
| MS.gene05666 | MS.gene69886 | 0.828484 | 8.76E-55 | -1.69E-46 |
| MS.gene056796 | MS.gene69886 | 0.811214 | 7.83E-51 | -1.69E-46 |
| MS.gene056897 | MS.gene69886 | 0.839115 | 1.92E-57 | -1.69E-46 |
| MS.gene057011 | MS.gene69886 | 0.834734 | 2.53E-56 | -1.69E-46 |
| MS.gene05702 | MS.gene69886 | 0.829819 | 4.16E-55 | -1.69E-46 |
| MS.gene057076 | MS.gene69886 | 0.817935 | 2.55E-52 | -1.69E-46 |
| MS.gene057124 | MS.gene69886 | 0.83343 | 5.36E-56 | -1.69E-46 |
| MS.gene057129 | MS.gene69886 | 0.880259 | 6.36E-70 | -1.69E-46 |
| MS.gene057146 | MS.gene69886 | 0.822391 | 2.43E-53 | -1.69E-46 |
| MS.gene057196 | MS.gene69886 | 0.840242 | 9.78E-58 | -1.69E-46 |
| MS.gene057295 | MS.gene69886 | 0.82629 | 2.94E-54 | -1.69E-46 |
| MS.gene057331 | MS.gene69886 | 0.883061 | 6.17E-71 | -1.69E-46 |
| MS.gene05735 | MS.gene69886 | 0.808319 | 3.28E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene69886 | 0.876658 | 1.17E-68 | -1.69E-46 |
| MS.gene057658 | MS.gene69886 | 0.802192 | 6.31E-49 | -1.69E-46 |
| MS.gene057734 | MS.gene69886 | 0.814246 | 1.70E-51 | -1.69E-46 |
| MS.gene057926 | MS.gene69886 | 0.817434 | 3.30E-52 | -1.69E-46 |
| MS.gene058006 | MS.gene69886 | 0.832635 | 8.45E-56 | -1.69E-46 |
| MS.gene058019 | MS.gene69886 | 0.839923 | 1.18E-57 | -1.69E-46 |
| MS.gene058082 | MS.gene69886 | 0.800654 | 1.30E-48 | -1.69E-46 |
| MS.gene058130 | MS.gene69886 | 0.808887 | 2.48E-50 | -1.69E-46 |
| MS.gene058233 | MS.gene69886 | 0.808831 | 2.55E-50 | -1.69E-46 |
| MS.gene058293 | MS.gene69886 | 0.802969 | 4.36E-49 | -1.69E-46 |
| MS.gene058323 | MS.gene69886 | 0.837387 | 5.36E-57 | -1.69E-46 |
| MS.gene058419 | MS.gene69886 | 0.847483 | 1.12E-59 | -1.69E-46 |
| MS.gene058544 | MS.gene69886 | 0.83345 | 5.30E-56 | -1.69E-46 |
| MS.gene058660 | MS.gene69886 | 0.852055 | 5.93E-61 | -1.69E-46 |
| MS.gene058723 | MS.gene69886 | 0.885482 | 7.83E-72 | -1.69E-46 |
| MS.gene058773 | MS.gene69886 | 0.820216 | 7.71E-53 | -1.69E-46 |
| MS.gene058838 | MS.gene69886 | 0.811305 | 7.48E-51 | -1.69E-46 |
| MS.gene05883 | MS.gene69886 | 0.837295 | 5.66E-57 | -1.69E-46 |
| MS.gene058928 | MS.gene69886 | 0.800492 | 1.41E-48 | -1.69E-46 |
| MS.gene059022 | MS.gene69886 | 0.812973 | 3.24E-51 | -1.69E-46 |
| MS.gene059029 | MS.gene69886 | 0.824451 | 8.02E-54 | -1.69E-46 |
| MS.gene05934 | MS.gene69886 | 0.838638 | 2.55E-57 | -1.69E-46 |
| MS.gene059536 | MS.gene69886 | 0.865394 | 6.10E-65 | -1.69E-46 |
| MS.gene059676 | MS.gene69886 | 0.80179 | 7.62E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene69886 | 0.832698 | 8.15E-56 | -1.69E-46 |
| MS.gene059688 | MS.gene69886 | 0.847181 | 1.36E-59 | -1.69E-46 |
| MS.gene059692 | MS.gene69886 | 0.84588 | 3.08E-59 | -1.69E-46 |
| MS.gene059695 | MS.gene69886 | 0.843962 | 1.01E-58 | -1.69E-46 |
| MS.gene059711 | MS.gene69886 | 0.803464 | 3.44E-49 | -1.69E-46 |
| MS.gene059718 | MS.gene69886 | 0.86067 | 1.76E-63 | -1.69E-46 |
| MS.gene059858 | MS.gene69886 | 0.805222 | 1.48E-49 | -1.69E-46 |
| MS.gene059902 | MS.gene69886 | 0.859343 | 4.42E-63 | -1.69E-46 |
| MS.gene059960 | MS.gene69886 | 0.855573 | 5.76E-62 | -1.69E-46 |
| MS.gene060193 | MS.gene69886 | 0.808958 | 2.40E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene69886 | 0.810841 | 9.43E-51 | -1.69E-46 |
| MS.gene060403 | MS.gene69886 | 0.847253 | 1.30E-59 | -1.69E-46 |
| MS.gene060469 | MS.gene69886 | 0.824078 | 9.81E-54 | -1.69E-46 |
| MS.gene060482 | MS.gene69886 | 0.828296 | 9.73E-55 | -1.69E-46 |
| MS.gene060762 | MS.gene69886 | 0.806149 | 9.46E-50 | -1.69E-46 |
| MS.gene060804 | MS.gene69886 | 0.899586 | 1.71E-77 | -1.69E-46 |
| MS.gene060818 | MS.gene69886 | 0.847741 | 9.54E-60 | -1.69E-46 |
| MS.gene060835 | MS.gene69886 | 0.840369 | 9.06E-58 | -1.69E-46 |
| MS.gene060837 | MS.gene69886 | 0.835131 | 2.01E-56 | -1.69E-46 |
| MS.gene06092 | MS.gene69886 | 0.840956 | 6.36E-58 | -1.69E-46 |
| MS.gene061041 | MS.gene69886 | 0.851022 | 1.16E-60 | -1.69E-46 |
| MS.gene061051 | MS.gene69886 | -0.823482 | 1.35E-53 | -1.69E-46 |
| MS.gene061150 | MS.gene69886 | 0.838931 | 2.14E-57 | -1.69E-46 |
| MS.gene061200 | MS.gene69886 | 0.859169 | 4.98E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69886.t1 | MTR_3g079520 | 93.548 | 217 | 14 | 0 | 1 | 217 | 1 | 217 | 1.54e-145 | 405 |
| MS.gene69886.t1 | MTR_3g479600 | 93.548 | 217 | 14 | 0 | 1 | 217 | 1 | 217 | 1.54e-145 | 405 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69886.t1 | AT2G21530 | 60.000 | 130 | 51 | 1 | 82 | 211 | 81 | 209 | 1.53e-51 | 166 |
Find 39 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATCTTCTTGTTATAGATT+TGG | 0.263843 | 3.4:-75865468 | MS.gene69886:CDS |
| TCTGGGAGTTATATTACTTT+TGG | 0.301923 | 3.4:-75865380 | MS.gene69886:intron |
| AATGCCAGGGGACACTAATT+TGG | 0.313943 | 3.4:-75865153 | MS.gene69886:intron |
| ACTTCTATACCTGTTGCAAC+TGG | 0.344018 | 3.4:+75865685 | None:intergenic |
| CCATTGTCTAGTTCACCTTC+TGG | 0.345009 | 3.4:+75865077 | None:intergenic |
| GCAGAACATGTTTGAGACTT+TGG | 0.358567 | 3.4:+75866367 | None:intergenic |
| GTAGTTACCACTGTATCTTC+TGG | 0.366490 | 3.4:-75865398 | MS.gene69886:CDS |
| CTTTGTGTAGCATTAGTTGT+TGG | 0.401469 | 3.4:+75866169 | None:intergenic |
| GCATTGGAGTGTAATAAAAT+TGG | 0.429187 | 3.4:+75866328 | None:intergenic |
| ATCACTAAATCAGCTTTCTC+TGG | 0.443107 | 3.4:+75865709 | None:intergenic |
| TTAGTGATCCCAGTTGCAAC+AGG | 0.462387 | 3.4:-75865694 | MS.gene69886:intron |
| TTCAAGGCATATAGGCTTCA+AGG | 0.462486 | 3.4:-75865864 | MS.gene69886:CDS |
| GATGGTGATTCAAGGCATAT+AGG | 0.467110 | 3.4:-75865872 | MS.gene69886:CDS |
| AGCACCAATGGAACATTCAT+TGG | 0.469041 | 3.4:-75865443 | MS.gene69886:CDS |
| GAACATTCATTGGTGACAAA+AGG | 0.471831 | 3.4:-75865433 | MS.gene69886:CDS |
| TAGTTACCACTGTATCTTCT+GGG | 0.476442 | 3.4:-75865397 | MS.gene69886:CDS |
| CAACTAATGCTACACAAAGA+TGG | 0.490326 | 3.4:-75866165 | MS.gene69886:CDS |
| TGATAGGAGATGGTGATTCA+AGG | 0.491582 | 3.4:-75865880 | MS.gene69886:intron |
| GAAAGTTACTGAAGTGGCAT+TGG | 0.511446 | 3.4:+75866312 | None:intergenic |
| CAGGTGTGCATGCACGCATT+CGG | 0.513668 | 3.4:-75865502 | MS.gene69886:CDS |
| GTCACCAATGAATGTTCCAT+TGG | 0.522297 | 3.4:+75865439 | None:intergenic |
| GAGAAGGCTGCAGATACAGT+TGG | 0.528528 | 3.4:-75865104 | MS.gene69886:CDS |
| GGTGACAAAAGGCTGAAACC+TGG | 0.545814 | 3.4:-75865422 | MS.gene69886:CDS |
| AGATGGATTCTTGAACCAGT+TGG | 0.550406 | 3.4:-75866148 | MS.gene69886:intron |
| ATAGATTTGGATAGCACCAA+TGG | 0.560750 | 3.4:-75865455 | MS.gene69886:CDS |
| GGCTTCAAGGTTGAGAGACC+AGG | 0.565164 | 3.4:-75865851 | MS.gene69886:CDS |
| GATACAGTGGTAACTACTCC+AGG | 0.567838 | 3.4:+75865404 | None:intergenic |
| TGTTATACATCCCAAATCAC+AGG | 0.568456 | 3.4:-75866236 | MS.gene69886:CDS |
| CTTCTATACCTGTTGCAACT+GGG | 0.577256 | 3.4:+75865686 | None:intergenic |
| TGTGTCGAAAATAGAAGAGA+AGG | 0.593642 | 3.4:-75865120 | MS.gene69886:CDS |
| TGTAGCATTAGTTGTTGGAG+TGG | 0.594055 | 3.4:+75866174 | None:intergenic |
| ACTCTAGCAACAATAATGGA+AGG | 0.614859 | 3.4:-75866421 | None:intergenic |
| ATCTAGAATGAAGTCACTGT+AGG | 0.636581 | 3.4:-75865739 | MS.gene69886:intron |
| TGAACTGAAAGTTACTGAAG+TGG | 0.650521 | 3.4:+75866306 | None:intergenic |
| GATACAGTTGGAGAACCAGA+AGG | 0.670779 | 3.4:-75865092 | MS.gene69886:CDS |
| GAAGTAATTTCATAAGCACC+TGG | 0.674584 | 3.4:+75865833 | None:intergenic |
| TTGGGATGTATAACACGACC+TGG | 0.687791 | 3.4:+75866244 | None:intergenic |
| ATAACTCCCAGAAGATACAG+TGG | 0.690459 | 3.4:+75865391 | None:intergenic |
| CCAGAAGGTGAACTAGACAA+TGG | 0.701962 | 3.4:-75865077 | MS.gene69886:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTATTATTACTACAAAT+AGG | - | chr3.4:75865445-75865464 | MS.gene69886:CDS | 10.0% |
| !! | TAGTAATAATAATATCATGA+AGG | + | chr3.4:75865440-75865459 | None:intergenic | 15.0% |
| !! | TTTAAGTATTTGTTTCAATA+TGG | - | chr3.4:75865488-75865507 | MS.gene69886:CDS | 15.0% |
| !!! | GATTTTAACAATTTTTCAAT+TGG | + | chr3.4:75865353-75865372 | None:intergenic | 15.0% |
| !! | ACATAATTATTCAACAGAAA+AGG | + | chr3.4:75866123-75866142 | None:intergenic | 20.0% |
| !! | TGAAACAAATACTTAAAAAG+TGG | + | chr3.4:75865485-75865504 | None:intergenic | 20.0% |
| !! | TTTGTAACTGTAAGAAAAAT+AGG | + | chr3.4:75865812-75865831 | None:intergenic | 20.0% |
| !!! | TATCATTTTGAAATTCTCTT+CGG | + | chr3.4:75866215-75866234 | None:intergenic | 20.0% |
| ! | ACTTAAAAAGTGGTTAATTG+AGG | + | chr3.4:75865475-75865494 | None:intergenic | 25.0% |
| ! | AGCATAGTACATTAAGATTA+AGG | + | chr3.4:75865858-75865877 | None:intergenic | 25.0% |
| ! | ATAATAGCAGAGGTTAAATA+TGG | + | chr3.4:75866266-75866285 | None:intergenic | 25.0% |
| ! | CAAATTATATTGTGATTCTC+TGG | + | chr3.4:75865892-75865911 | None:intergenic | 25.0% |
| ! | CAATATGGTCAATATTAACA+AGG | - | chr3.4:75865503-75865522 | MS.gene69886:CDS | 25.0% |
| ! | CTCAATCAAGATAAGAAAAA+TGG | + | chr3.4:75865399-75865418 | None:intergenic | 25.0% |
| ! | CTTAAAAAGTGGTTAATTGA+GGG | + | chr3.4:75865474-75865493 | None:intergenic | 25.0% |
| !! | CAATCTTCTTGTTATAGATT+TGG | - | chr3.4:75865972-75865991 | MS.gene69886:intron | 25.0% |
| !!! | ACAAAACCAATCATTTTGAT+AGG | - | chr3.4:75865544-75865563 | MS.gene69886:intron | 25.0% |
| CAGAGGTTAAATATGGAATA+GGG | + | chr3.4:75866259-75866278 | None:intergenic | 30.0% | |
| GCATTGGAGTGTAATAAAAT+TGG | + | chr3.4:75865115-75865134 | None:intergenic | 30.0% | |
| TATATAACCCTGTTTGTAGA+AGG | - | chr3.4:75866169-75866188 | MS.gene69886:CDS | 30.0% | |
| TTAAGATCCTTCTACAAACA+GGG | + | chr3.4:75866179-75866198 | None:intergenic | 30.0% | |
| TTGAAATTCTCTTCGGAATA+TGG | + | chr3.4:75866208-75866227 | None:intergenic | 30.0% | |
| ! | ATCAGAATCAATGATTTGTG+TGG | - | chr3.4:75865640-75865659 | MS.gene69886:intron | 30.0% |
| ! | TCTGGGAGTTATATTACTTT+TGG | - | chr3.4:75866060-75866079 | MS.gene69886:intron | 30.0% |
| ! | TGTTTTGTATTTACAGTGTC+AGG | - | chr3.4:75865919-75865938 | MS.gene69886:intron | 30.0% |
| ! | TTTAAGATCCTTCTACAAAC+AGG | + | chr3.4:75866180-75866199 | None:intergenic | 30.0% |
| !! | AACAAGGTGTTTTATCACTT+TGG | - | chr3.4:75865519-75865538 | MS.gene69886:intron | 30.0% |
| !!! | GAGACTTTGGAATTTTTGTT+AGG | + | chr3.4:75865063-75865082 | None:intergenic | 30.0% |
| !!! | TTCACTTTTTCCTGTGATTT+GGG | + | chr3.4:75865217-75865236 | None:intergenic | 30.0% |
| ATAGATTTGGATAGCACCAA+TGG | - | chr3.4:75865985-75866004 | MS.gene69886:intron | 35.0% | |
| ATCACTAAATCAGCTTTCTC+TGG | + | chr3.4:75865734-75865753 | None:intergenic | 35.0% | |
| CAACTAATGCTACACAAAGA+TGG | - | chr3.4:75865275-75865294 | MS.gene69886:intron | 35.0% | |
| CCATCTCCTATCAAAATGAT+TGG | + | chr3.4:75865553-75865572 | None:intergenic | 35.0% | |
| CTTTGTGTAGCATTAGTTGT+TGG | + | chr3.4:75865274-75865293 | None:intergenic | 35.0% | |
| GAAGTAATTTCATAAGCACC+TGG | + | chr3.4:75865610-75865629 | None:intergenic | 35.0% | |
| GATTAAGGAGCTTATTTCAG+TGG | + | chr3.4:75865843-75865862 | None:intergenic | 35.0% | |
| GCAGAGGTTAAATATGGAAT+AGG | + | chr3.4:75866260-75866279 | None:intergenic | 35.0% | |
| TAGTTACCACTGTATCTTCT+GGG | - | chr3.4:75866043-75866062 | MS.gene69886:intron | 35.0% | |
| TGAACTGAAAGTTACTGAAG+TGG | + | chr3.4:75865137-75865156 | None:intergenic | 35.0% | |
| TGTGTCGAAAATAGAAGAGA+AGG | - | chr3.4:75866320-75866339 | MS.gene69886:CDS | 35.0% | |
| TGTTATACATCCCAAATCAC+AGG | - | chr3.4:75865204-75865223 | MS.gene69886:intron | 35.0% | |
| ! | AGAAAGTGTAACTAACCAAC+TGG | + | chr3.4:75865310-75865329 | None:intergenic | 35.0% |
| ! | ATCTAGAATGAAGTCACTGT+AGG | - | chr3.4:75865701-75865720 | MS.gene69886:CDS | 35.0% |
| ! | GAACATTCATTGGTGACAAA+AGG | - | chr3.4:75866007-75866026 | MS.gene69886:intron | 35.0% |
| ! | TCTCTTCTATTTTCGACACA+CGG | + | chr3.4:75866320-75866339 | None:intergenic | 35.0% |
| !! | CCAATCATTTTGATAGGAGA+TGG | - | chr3.4:75865550-75865569 | MS.gene69886:intron | 35.0% |
| !!! | CTTCACTTTTTCCTGTGATT+TGG | + | chr3.4:75865218-75865237 | None:intergenic | 35.0% |
| ACTTCTATACCTGTTGCAAC+TGG | + | chr3.4:75865758-75865777 | None:intergenic | 40.0% | |
| AGCACCAATGGAACATTCAT+TGG | - | chr3.4:75865997-75866016 | MS.gene69886:intron | 40.0% | |
| ATAACTCCCAGAAGATACAG+TGG | + | chr3.4:75866052-75866071 | None:intergenic | 40.0% | |
| CTCTGCTATTATGAATGCCA+GGG | - | chr3.4:75866274-75866293 | MS.gene69886:CDS | 40.0% | |
| CTTCTATACCTGTTGCAACT+GGG | + | chr3.4:75865757-75865776 | None:intergenic | 40.0% | |
| GAAAGTTACTGAAGTGGCAT+TGG | + | chr3.4:75865131-75865150 | None:intergenic | 40.0% | |
| GATGGTGATTCAAGGCATAT+AGG | - | chr3.4:75865568-75865587 | MS.gene69886:intron | 40.0% | |
| GTAGTTACCACTGTATCTTC+TGG | - | chr3.4:75866042-75866061 | MS.gene69886:intron | 40.0% | |
| GTCACCAATGAATGTTCCAT+TGG | + | chr3.4:75866004-75866023 | None:intergenic | 40.0% | |
| TCACAGGAAAAAGTGAAGCT+AGG | - | chr3.4:75865220-75865239 | MS.gene69886:intron | 40.0% | |
| TCTGCTATTATGAATGCCAG+GGG | - | chr3.4:75866275-75866294 | MS.gene69886:CDS | 40.0% | |
| TGATAGGAGATGGTGATTCA+AGG | - | chr3.4:75865560-75865579 | MS.gene69886:intron | 40.0% | |
| TGTAGCATTAGTTGTTGGAG+TGG | + | chr3.4:75865269-75865288 | None:intergenic | 40.0% | |
| TTCAAGGCATATAGGCTTCA+AGG | - | chr3.4:75865576-75865595 | MS.gene69886:intron | 40.0% | |
| ! | AGATGGATTCTTGAACCAGT+TGG | - | chr3.4:75865292-75865311 | MS.gene69886:intron | 40.0% |
| ! | GCAGAACATGTTTGAGACTT+TGG | + | chr3.4:75865076-75865095 | None:intergenic | 40.0% |
| !!! | TGCTTTCTTTTTCAGCAACC+AGG | - | chr3.4:75865178-75865197 | MS.gene69886:intron | 40.0% |
| AATGCCAGGGGACACTAATT+TGG | - | chr3.4:75866287-75866306 | MS.gene69886:CDS | 45.0% | |
| ATAGCCAAATTAGTGTCCCC+TGG | + | chr3.4:75866294-75866313 | None:intergenic | 45.0% | |
| CCAGAAGGTGAACTAGACAA+TGG | - | chr3.4:75866363-75866382 | MS.gene69886:CDS | 45.0% | |
| CCTCTGCTATTATGAATGCC+AGG | - | chr3.4:75866273-75866292 | MS.gene69886:CDS | 45.0% | |
| CCTGGCATTCATAATAGCAG+AGG | + | chr3.4:75866276-75866295 | None:intergenic | 45.0% | |
| GATACAGTGGTAACTACTCC+AGG | + | chr3.4:75866039-75866058 | None:intergenic | 45.0% | |
| GATACAGTTGGAGAACCAGA+AGG | - | chr3.4:75866348-75866367 | MS.gene69886:CDS | 45.0% | |
| TTAGTGATCCCAGTTGCAAC+AGG | - | chr3.4:75865746-75865765 | MS.gene69886:intron | 45.0% | |
| TTGGGATGTATAACACGACC+TGG | + | chr3.4:75865199-75865218 | None:intergenic | 45.0% | |
| ! | CCATTGTCTAGTTCACCTTC+TGG | + | chr3.4:75866366-75866385 | None:intergenic | 45.0% |
| GAGAAGGCTGCAGATACAGT+TGG | - | chr3.4:75866336-75866355 | MS.gene69886:CDS | 50.0% | |
| GGTGACAAAAGGCTGAAACC+TGG | - | chr3.4:75866018-75866037 | MS.gene69886:intron | 50.0% | |
| CAGGTGTGCATGCACGCATT+CGG | - | chr3.4:75865938-75865957 | MS.gene69886:intron | 55.0% | |
| GGCTTCAAGGTTGAGAGACC+AGG | - | chr3.4:75865589-75865608 | MS.gene69886:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 75865034 | 75866428 | 75865034 | ID=MS.gene69886 |
| chr3.4 | mRNA | 75865034 | 75866428 | 75865034 | ID=MS.gene69886.t1;Parent=MS.gene69886 |
| chr3.4 | exon | 75866149 | 75866428 | 75866149 | ID=MS.gene69886.t1.exon1;Parent=MS.gene69886.t1 |
| chr3.4 | CDS | 75866149 | 75866428 | 75866149 | ID=cds.MS.gene69886.t1;Parent=MS.gene69886.t1 |
| chr3.4 | exon | 75865832 | 75865896 | 75865832 | ID=MS.gene69886.t1.exon2;Parent=MS.gene69886.t1 |
| chr3.4 | CDS | 75865832 | 75865896 | 75865832 | ID=cds.MS.gene69886.t1;Parent=MS.gene69886.t1 |
| chr3.4 | exon | 75865695 | 75865755 | 75865695 | ID=MS.gene69886.t1.exon3;Parent=MS.gene69886.t1 |
| chr3.4 | CDS | 75865695 | 75865755 | 75865695 | ID=cds.MS.gene69886.t1;Parent=MS.gene69886.t1 |
| chr3.4 | exon | 75865381 | 75865527 | 75865381 | ID=MS.gene69886.t1.exon4;Parent=MS.gene69886.t1 |
| chr3.4 | CDS | 75865381 | 75865527 | 75865381 | ID=cds.MS.gene69886.t1;Parent=MS.gene69886.t1 |
| chr3.4 | exon | 75865034 | 75865167 | 75865034 | ID=MS.gene69886.t1.exon5;Parent=MS.gene69886.t1 |
| chr3.4 | CDS | 75865034 | 75865167 | 75865034 | ID=cds.MS.gene69886.t1;Parent=MS.gene69886.t1 |
| Gene Sequence |
| Protein sequence |
