AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002981.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002981.01.T01 MTR_1g054055 88.050 159 10 4 11 168 1 151 1.78e-82 240
MsG0180002981.01.T01 MTR_2g061720 38.514 148 77 3 3 150 21 154 1.06e-23 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002981.01.T01 AT3G58000 39.735 151 69 5 1 151 18 146 2.36e-24 93.6
MsG0180002981.01.T01 AT2G42140 38.562 153 70 4 1 152 19 148 4.61e-23 90.1
MsG0180002981.01.T01 AT2G44340 36.806 144 65 4 1 143 19 137 4.79e-19 80.1
MsG0180002981.01.T01 AT3G60090 72.727 44 12 0 1 44 31 74 4.97e-16 71.6

Find 26 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTGAATTATTCGTATCTT+AGG 0.207900 1:-54252419 None:intergenic
GAGAGATGTGGTGGCTATTT+AGG 0.256894 1:+54252752 MsG0180002981.01.T01:CDS
GAACTTGTTCAGAAGTTAAC+TGG 0.301545 1:+54252485 MsG0180002981.01.T01:CDS
GGGTTTATTTCTGAAATTAA+TGG 0.329702 1:+54252794 MsG0180002981.01.T01:CDS
TGGATTTCCTTTGTTTCCCT+TGG 0.330042 1:+54252817 MsG0180002981.01.T01:CDS
AAGACAAGGGTAATTGTAGA+AGG 0.455330 1:+54252545 MsG0180002981.01.T01:CDS
GATGTGGTGGCTATTTAGGT+GGG 0.464069 1:+54252756 MsG0180002981.01.T01:CDS
TAATCTTTGTCTTCATCACT+TGG 0.466152 1:-54252512 None:intergenic
GTCAAAGAAGAAGTAGGTGG+TGG 0.492033 1:+54252719 MsG0180002981.01.T01:CDS
AGATGTGGTGGCTATTTAGG+TGG 0.506681 1:+54252755 MsG0180002981.01.T01:CDS
GATAAAGTCAAAGAAGAAGT+AGG 0.508334 1:+54252713 MsG0180002981.01.T01:CDS
TTTATTTCTGAAATTAATGG+TGG 0.513856 1:+54252797 MsG0180002981.01.T01:CDS
ATTGTGAGTGGCATGATCCA+AGG 0.542951 1:-54252834 None:intergenic
AGCACTACAAGCAGTGGCAG+TGG 0.554832 1:+54252605 MsG0180002981.01.T01:CDS
CATGCCACTCACAATATGCA+AGG 0.590941 1:+54252842 MsG0180002981.01.T01:CDS
TGGCAGTGGTTTATCATCAG+AGG 0.596180 1:+54252619 MsG0180002981.01.T01:CDS
TTGTGAGTGGCATGATCCAA+GGG 0.601677 1:-54252833 None:intergenic
GCATGATCCAAGGGAAACAA+AGG 0.604304 1:-54252824 None:intergenic
ACACCCTTGCATATTGTGAG+TGG 0.617076 1:-54252846 None:intergenic
TCAAGAAGCACTACAAGCAG+TGG 0.632435 1:+54252599 MsG0180002981.01.T01:CDS
ATTATTTCAAGAAGAAGACA+AGG 0.634299 1:+54252531 MsG0180002981.01.T01:CDS
AAAGTCAAAGAAGAAGTAGG+TGG 0.660011 1:+54252716 MsG0180002981.01.T01:CDS
ATGCCACTCACAATATGCAA+GGG 0.664055 1:+54252843 MsG0180002981.01.T01:CDS
AGCAGTGATGAGAGATGTGG+TGG 0.685118 1:+54252743 MsG0180002981.01.T01:CDS
GGAAGCAGTGATGAGAGATG+TGG 0.705953 1:+54252740 MsG0180002981.01.T01:CDS
TTATTTCAAGAAGAAGACAA+GGG 0.717654 1:+54252532 MsG0180002981.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGATAACAATAAAAAATG+AGG + Chr1:54252652-54252671 MsG0180002981.01.T01:CDS 15.0%
!!! TTTCATTTTTGATATTTTGT+GGG - Chr1:54252399-54252418 None:intergenic 15.0%
!!! GTTTCATTTTTGATATTTTG+TGG - Chr1:54252400-54252419 None:intergenic 20.0%
!!! TTTATTTCTGAAATTAATGG+TGG + Chr1:54252797-54252816 MsG0180002981.01.T01:CDS 20.0%
! ATAAAAAATGAGGTGAGTAA+TGG + Chr1:54252662-54252681 MsG0180002981.01.T01:CDS 25.0%
! ATGTGAATTATTCGTATCTT+AGG - Chr1:54252422-54252441 None:intergenic 25.0%
! ATTATTTCAAGAAGAAGACA+AGG + Chr1:54252531-54252550 MsG0180002981.01.T01:CDS 25.0%
! TTATTTCAAGAAGAAGACAA+GGG + Chr1:54252532-54252551 MsG0180002981.01.T01:CDS 25.0%
!!! GGGTTTATTTCTGAAATTAA+TGG + Chr1:54252794-54252813 MsG0180002981.01.T01:CDS 25.0%
GATAAAGTCAAAGAAGAAGT+AGG + Chr1:54252713-54252732 MsG0180002981.01.T01:CDS 30.0%
GTAGAAGGATCAAAAAATGA+AGG + Chr1:54252560-54252579 MsG0180002981.01.T01:CDS 30.0%
TAATCTTTGTCTTCATCACT+TGG - Chr1:54252515-54252534 None:intergenic 30.0%
! AAACTGATGCTGAGAATTTT+AGG + Chr1:54252462-54252481 MsG0180002981.01.T01:CDS 30.0%
! AACTGATGCTGAGAATTTTA+GGG + Chr1:54252463-54252482 MsG0180002981.01.T01:CDS 30.0%
AAAAATGAGGTGAGTAATGG+TGG + Chr1:54252665-54252684 MsG0180002981.01.T01:CDS 35.0%
AAAGTCAAAGAAGAAGTAGG+TGG + Chr1:54252716-54252735 MsG0180002981.01.T01:CDS 35.0%
AAGACAAGGGTAATTGTAGA+AGG + Chr1:54252545-54252564 MsG0180002981.01.T01:CDS 35.0%
! GAACTTGTTCAGAAGTTAAC+TGG + Chr1:54252485-54252504 MsG0180002981.01.T01:CDS 35.0%
!! AGCATCAGTTTTGATGATCT+CGG - Chr1:54252453-54252472 None:intergenic 35.0%
!! TTTAGGTGGGTTTTCAGATT+TGG + Chr1:54252769-54252788 MsG0180002981.01.T01:CDS 35.0%
ATGCCACTCACAATATGCAA+GGG + Chr1:54252843-54252862 MsG0180002981.01.T01:CDS 40.0%
! AGGTGAGTAATGGTGGTTTT+TGG + Chr1:54252672-54252691 MsG0180002981.01.T01:CDS 40.0%
! GGTGAGTAATGGTGGTTTTT+GGG + Chr1:54252673-54252692 MsG0180002981.01.T01:CDS 40.0%
! GTGAGTAATGGTGGTTTTTG+GGG + Chr1:54252674-54252693 MsG0180002981.01.T01:CDS 40.0%
! GTGGGTTTTCAGATTTGGAA+GGG + Chr1:54252774-54252793 MsG0180002981.01.T01:CDS 40.0%
! TGGATTTCCTTTGTTTCCCT+TGG + Chr1:54252817-54252836 MsG0180002981.01.T01:CDS 40.0%
!! GCATCAGTTTTGATGATCTC+GGG - Chr1:54252452-54252471 None:intergenic 40.0%
!! TGAGTAATGGTGGTTTTTGG+GGG + Chr1:54252675-54252694 MsG0180002981.01.T01:CDS 40.0%
ACACCCTTGCATATTGTGAG+TGG - Chr1:54252849-54252868 None:intergenic 45.0%
AGATGTGGTGGCTATTTAGG+TGG + Chr1:54252755-54252774 MsG0180002981.01.T01:CDS 45.0%
ATTGTGAGTGGCATGATCCA+AGG - Chr1:54252837-54252856 None:intergenic 45.0%
CATGCCACTCACAATATGCA+AGG + Chr1:54252842-54252861 MsG0180002981.01.T01:CDS 45.0%
GAGAGATGTGGTGGCTATTT+AGG + Chr1:54252752-54252771 MsG0180002981.01.T01:CDS 45.0%
GATGTGGTGGCTATTTAGGT+GGG + Chr1:54252756-54252775 MsG0180002981.01.T01:CDS 45.0%
GCATGATCCAAGGGAAACAA+AGG - Chr1:54252827-54252846 None:intergenic 45.0%
GTCAAAGAAGAAGTAGGTGG+TGG + Chr1:54252719-54252738 MsG0180002981.01.T01:CDS 45.0%
TGGCAGTGGTTTATCATCAG+AGG + Chr1:54252619-54252638 MsG0180002981.01.T01:CDS 45.0%
TTGTGAGTGGCATGATCCAA+GGG - Chr1:54252836-54252855 None:intergenic 45.0%
!! GGTGGGTTTTCAGATTTGGA+AGG + Chr1:54252773-54252792 MsG0180002981.01.T01:CDS 45.0%
!! TAATGGTGGTTTTTGGGGGT+TGG + Chr1:54252679-54252698 MsG0180002981.01.T01:CDS 45.0%
!! TCAAGAAGCACTACAAGCAG+TGG + Chr1:54252599-54252618 MsG0180002981.01.T01:CDS 45.0%
AGCAGTGATGAGAGATGTGG+TGG + Chr1:54252743-54252762 MsG0180002981.01.T01:CDS 50.0%
GGAAGCAGTGATGAGAGATG+TGG + Chr1:54252740-54252759 MsG0180002981.01.T01:CDS 50.0%
TTGGGGGTTGGATCTGACAA+TGG + Chr1:54252691-54252710 MsG0180002981.01.T01:CDS 50.0%
AGCACTACAAGCAGTGGCAG+TGG + Chr1:54252605-54252624 MsG0180002981.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 54252383 54252889 54252383 ID=MsG0180002981.01;Name=MsG0180002981.01
Chr1 mRNA 54252383 54252889 54252383 ID=MsG0180002981.01.T01;Parent=MsG0180002981.01;Name=MsG0180002981.01.T01;_AED=0.21;_eAED=0.21;_QI=0|-1|0|1|-1|1|1|0|168
Chr1 exon 54252383 54252889 54252383 ID=MsG0180002981.01.T01:exon:22241;Parent=MsG0180002981.01.T01
Chr1 CDS 54252383 54252889 54252383 ID=MsG0180002981.01.T01:cds;Parent=MsG0180002981.01.T01
Gene Sequence

>MsG0180002981.01.T01

ATGCATAAAGATTCCCACAAAATATCAAAAATGAAACCTAAGATACGAATAATTCACATATATGCACCCGAGATCATCAAAACTGATGCTGAGAATTTTAGGGAACTTGTTCAGAAGTTAACTGGAAAACCAAGTGATGAAGACAAAGATTATTTCAAGAAGAAGACAAGGGTAATTGTAGAAGGATCAAAAAATGAAGGAAGAGAGCAAGAATCTTCAAGAAGCACTACAAGCAGTGGCAGTGGTTTATCATCAGAGGATTGTTCAAGAATGATAACAATAAAAAATGAGGTGAGTAATGGTGGTTTTTGGGGGTTGGATCTGACAATGGATAAAGTCAAAGAAGAAGTAGGTGGTGGAAGCAGTGATGAGAGATGTGGTGGCTATTTAGGTGGGTTTTCAGATTTGGAAGGGTTTATTTCTGAAATTAATGGTGGATTTCCTTTGTTTCCCTTGGATCATGCCACTCACAATATGCAAGGGTGTGAACAATATCAACTACTATAA

Protein sequence

>MsG0180002981.01.T01

MHKDSHKISKMKPKIRIIHIYAPEIIKTDAENFRELVQKLTGKPSDEDKDYFKKKTRVIVEGSKNEGREQESSRSTTSSGSGLSSEDCSRMITIKNEVSNGGFWGLDLTMDKVKEEVGGGSSDERCGGYLGGFSDLEGFISEINGGFPLFPLDHATHNMQGCEQYQLL*