AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0713960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0713960 MtrunA17_Chr5g0401301 95.943 419 13 2 1 417 1 417 0.0 810
Msa0713960 MtrunA17_Chr1g0177171 27.816 435 265 14 23 414 62 490 7.38e-38 143
Msa0713960 MtrunA17_Chr3g0120401 61.538 91 33 2 324 414 152 240 6.57e-32 121
Msa0713960 MtrunA17_Chr1g0213651 60.215 93 35 2 323 415 270 360 5.49e-31 122
Msa0713960 MtrunA17_Chr7g0234821 24.018 433 262 11 24 414 51 458 7.79e-28 114
Msa0713960 MtrunA17_Chr8g0339191 25.000 428 253 16 28 414 58 458 1.19e-26 111
Msa0713960 MtrunA17_Chr5g0439251 53.191 94 37 2 329 416 382 474 2.72e-25 107
Msa0713960 MtrunA17_Chr5g0447651 54.545 88 39 1 327 414 28 114 1.48e-24 97.8
Msa0713960 MtrunA17_Chr8g0378361 52.326 86 39 2 329 414 30 113 2.58e-23 96.3
Msa0713960 MtrunA17_Chr1g0193131 28.855 454 226 23 37 408 58 496 5.59e-23 101
Msa0713960 MtrunA17_Chr3g0096451 50.000 86 41 2 329 414 112 195 1.06e-22 96.7
Msa0713960 MtrunA17_Chr7g0271411 53.488 86 38 2 329 414 160 243 1.55e-22 97.1
Msa0713960 MtrunA17_Chr8g0378621 49.425 87 43 1 328 414 363 448 2.67e-22 99.0
Msa0713960 MtrunA17_Chr1g0161011 24.171 422 278 11 17 414 42 445 4.84e-22 98.2
Msa0713960 MtrunA17_Chr8g0378371 49.462 93 42 3 322 414 26 113 7.75e-22 90.1
Msa0713960 MtrunA17_Chr8g0381081 51.163 86 41 1 329 414 57 141 1.24e-21 91.7
Msa0713960 MtrunA17_Chr5g0443331 50.000 86 41 2 329 414 108 191 1.62e-21 93.6
Msa0713960 MtrunA17_Chr4g0076141 47.826 92 47 1 319 410 3 93 1.37e-20 86.3
Msa0713960 MtrunA17_Chr4g0075031 47.727 88 45 1 327 414 380 466 4.01e-20 92.4
Msa0713960 MtrunA17_Chr8g0378381 45.455 88 46 2 327 414 30 115 1.36e-19 84.0
Msa0713960 MtrunA17_Chr4g0076131 45.455 88 43 2 329 414 28 112 1.57e-19 84.0
Msa0713960 MtrunA17_Chr8g0389741 43.678 87 48 1 329 415 368 453 3.54e-19 89.4
Msa0713960 MtrunA17_Chr2g0304061 51.685 89 40 3 329 417 158 243 7.67e-19 87.0
Msa0713960 MtrunA17_Chr4g0040911 46.067 89 47 1 326 414 34 121 9.23e-19 83.6
Msa0713960 MtrunA17_Chr4g0046311 23.445 418 265 13 28 408 52 451 1.26e-18 87.8
Msa0713960 MtrunA17_Chr4g0061401 49.462 93 40 4 324 414 19 106 3.05e-18 83.2
Msa0713960 MtrunA17_Chr6g0487261 43.182 88 48 2 329 415 369 455 4.11e-18 86.3
Msa0713960 MtrunA17_Chr3g0121271 44.318 88 48 1 327 414 360 446 5.75e-18 85.9
Msa0713960 MtrunA17_Chr8g0378401 47.561 82 41 2 327 408 30 109 9.69e-18 79.0
Msa0713960 MtrunA17_Chr4g0034901 40.000 110 60 3 310 414 351 459 7.30e-17 82.4
Msa0713960 MtrunA17_Chr4g0043511 44.318 88 47 2 327 414 418 503 1.18e-16 82.0
Msa0713960 MtrunA17_Chr8g0380231 55.882 68 28 2 341 408 29 94 1.37e-16 75.1
Msa0713960 MtrunA17_Chr1g0154991 44.186 86 46 2 329 414 20 103 1.95e-16 74.7
Msa0713960 MtrunA17_Chr5g0434591 41.573 89 51 1 326 414 357 444 3.51e-16 80.5
Msa0713960 MtrunA17_Chr5g0419121 50.685 73 34 2 328 400 21 91 1.28e-15 76.3
Msa0713960 MtrunA17_Chr4g0043151 44.444 81 43 2 328 408 20 98 8.64e-15 72.8
Msa0713960 MtrunA17_Chr3g0144531 41.860 86 48 2 329 414 371 454 1.84e-14 75.1
Msa0713960 MtrunA17_Chr3g0110371 42.529 87 47 2 329 414 361 445 2.25e-14 74.7
Msa0713960 MtrunA17_Chr2g0313891 39.080 87 50 1 326 412 27 110 5.68e-13 65.1
Msa0713960 MtrunA17_Chr7g0223961 35.484 93 58 2 321 413 357 447 4.37e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0713960 AT5G67460.1 42.494 433 171 18 6 417 5 380 5.58e-82 257
Msa0713960 AT5G67460.2 41.920 427 170 18 6 411 5 374 1.29e-76 243
Msa0713960 AT5G55180.1 29.040 427 247 16 23 414 51 456 3.10e-37 141
Msa0713960 AT4G26830.1 27.273 418 263 11 23 414 49 451 1.02e-35 137
Msa0713960 AT4G26830.2 27.751 418 261 12 23 414 53 455 1.21e-35 137
Msa0713960 AT5G55180.2 29.216 421 242 16 23 408 51 450 2.60e-35 136
Msa0713960 AT1G79480.1 58.065 93 37 2 322 414 304 394 1.43e-30 121
Msa0713960 AT1G79480.2 58.065 93 37 2 322 414 303 393 1.43e-30 121
Msa0713960 AT2G01630.1 24.941 421 278 11 16 414 40 444 5.74e-27 113
Msa0713960 AT2G30933.1 57.143 91 37 2 324 414 77 165 1.25e-26 107
Msa0713960 AT2G05790.1 56.977 86 36 1 329 414 384 468 2.06e-26 111
Msa0713960 AT2G01630.3 24.641 418 273 11 16 409 40 439 3.31e-26 110
Msa0713960 AT2G30933.2 58.824 85 33 2 324 408 77 159 2.20e-25 101
Msa0713960 AT2G30933.3 58.824 85 33 2 324 408 77 159 2.20e-25 101
Msa0713960 AT1G11820.2 25.000 408 280 10 23 414 68 465 1.58e-24 105
Msa0713960 AT3G07320.1 24.594 431 260 17 24 414 50 455 3.39e-24 104
Msa0713960 AT5G35740.1 48.837 86 44 0 329 414 30 115 5.44e-24 96.7
Msa0713960 AT1G11820.3 24.205 409 274 10 23 410 68 461 1.02e-23 103
Msa0713960 AT1G66855.1 45.455 88 47 1 327 414 24 110 8.93e-23 92.8
Msa0713960 AT2G04910.2 47.674 86 44 1 329 414 15 99 1.28e-21 89.7
Msa0713960 AT4G05430.1 46.512 86 45 1 329 414 23 107 2.11e-21 90.1
Msa0713960 AT4G05430.3 46.512 86 45 1 329 414 48 132 2.97e-21 90.5
Msa0713960 AT1G29380.3 54.023 87 36 3 329 414 148 231 5.03e-21 92.4
Msa0713960 AT1G29380.1 54.023 87 36 3 329 414 148 231 5.88e-21 93.2
Msa0713960 AT2G04910.1 48.750 80 40 1 329 408 15 93 7.54e-21 88.2
Msa0713960 AT1G66870.1 43.182 88 49 1 327 414 24 110 8.63e-21 87.4
Msa0713960 AT1G66870.2 43.182 88 49 1 327 414 51 137 1.38e-20 87.8
Msa0713960 AT5G61130.1 48.837 86 42 2 329 414 21 104 2.28e-20 89.0
Msa0713960 AT4G13600.1 49.425 87 42 2 328 414 60 144 2.66e-20 89.7
Msa0713960 AT1G18650.1 51.163 86 40 2 329 414 21 104 2.81e-20 88.2
Msa0713960 AT4G05430.2 48.750 80 40 1 329 408 48 126 2.97e-20 86.7
Msa0713960 AT1G18650.2 51.163 86 40 2 329 414 21 104 3.68e-20 86.3
Msa0713960 AT1G09460.1 52.326 86 37 3 329 414 137 218 4.61e-20 90.9
Msa0713960 AT1G29380.4 55.556 81 32 3 329 408 148 225 5.60e-20 88.6
Msa0713960 AT1G29380.2 55.556 81 32 3 329 408 148 225 5.60e-20 88.6
Msa0713960 AT3G13560.4 46.667 90 47 1 328 417 361 449 1.65e-19 90.9
Msa0713960 AT3G13560.1 46.667 90 47 1 328 417 361 449 1.65e-19 90.9
Msa0713960 AT3G13560.2 46.667 90 47 1 328 417 361 449 1.65e-19 90.9
Msa0713960 AT3G13560.5 46.667 90 47 1 328 417 361 449 1.65e-19 90.9
Msa0713960 AT3G13560.3 46.667 90 47 1 328 417 361 449 1.65e-19 90.9
Msa0713960 AT5G56590.1 43.678 87 48 1 329 415 369 454 8.04e-19 89.0
Msa0713960 AT5G56590.1 28.571 154 99 4 22 168 49 198 1.06e-11 67.0
Msa0713960 AT5G08000.2 48.889 90 44 2 325 414 36 123 3.35e-18 83.2
Msa0713960 AT5G08000.1 50.000 86 41 2 329 414 21 104 6.49e-18 82.0
Msa0713960 AT1G66852.1 39.773 88 51 2 327 414 24 109 2.13e-17 78.2
Msa0713960 AT4G29360.1 39.560 91 54 1 327 417 389 478 2.59e-17 84.3
Msa0713960 AT4G29360.1 30.464 151 95 3 24 168 52 198 4.75e-11 65.1
Msa0713960 AT4G29360.2 39.560 91 54 1 327 417 399 488 2.72e-17 84.3
Msa0713960 AT4G29360.2 30.464 151 95 3 24 168 62 208 4.94e-11 65.1
Msa0713960 AT3G55430.1 34.906 106 67 2 309 414 346 449 3.18e-17 84.0
Msa0713960 AT5G63225.1 45.238 84 44 2 329 412 25 106 9.34e-17 76.3
Msa0713960 AT5G63230.1 40.909 88 50 2 327 414 24 109 2.07e-16 75.1
Msa0713960 AT4G16165.1 35.227 88 56 1 327 414 24 110 4.46e-16 74.3
Msa0713960 AT4G09090.1 40.230 87 50 2 329 415 31 115 8.98e-16 73.6
Msa0713960 AT4G09462.1 38.636 88 52 2 327 414 27 112 1.38e-15 73.2
Msa0713960 AT4G09465.1 38.636 88 52 2 327 414 29 114 1.56e-15 72.8
Msa0713960 AT4G09464.1 38.636 88 52 2 327 414 29 114 1.84e-15 72.8
Msa0713960 AT4G09467.1 38.636 88 52 2 327 414 29 114 1.84e-15 72.8
Msa0713960 AT5G63250.1 40.367 109 57 4 309 415 27 129 3.29e-15 72.4
Msa0713960 AT2G43670.1 40.476 84 47 2 329 412 35 115 4.11e-15 72.0
Msa0713960 AT4G09466.1 37.500 88 53 2 327 414 29 114 5.62e-15 71.2
Msa0713960 AT5G53610.1 44.186 86 45 2 329 414 28 110 8.76e-15 70.5
Msa0713960 AT2G29735.1 40.000 90 50 3 327 414 29 116 1.31e-14 70.5
Msa0713960 AT3G23770.1 39.080 87 52 1 328 414 386 471 1.65e-14 75.5
Msa0713960 AT1G26450.1 49.315 73 34 3 330 402 22 91 1.89e-14 72.0
Msa0713960 AT4G34480.3 41.860 86 48 2 329 414 253 336 2.66e-14 74.7
Msa0713960 AT4G34480.1 41.860 86 48 2 329 414 364 447 2.95e-14 74.7
Msa0713960 AT1G66250.1 39.326 89 53 1 326 414 365 452 3.06e-14 74.7
Msa0713960 AT3G58100.1 39.773 88 52 1 327 414 39 125 6.78e-14 70.1
Msa0713960 AT1G69295.1 50.000 72 33 3 329 400 21 89 6.80e-14 71.2
Msa0713960 AT1G69295.2 50.000 72 33 3 329 400 17 85 8.72e-14 70.9
Msa0713960 AT5G24318.3 40.909 88 50 2 327 414 231 316 1.00e-13 72.0
Msa0713960 AT5G24318.1 40.909 88 50 2 327 414 370 455 1.11e-13 72.8
Msa0713960 AT3G58100.2 39.773 88 52 1 327 414 115 201 1.40e-13 70.9
Msa0713960 AT5G63240.1 32.381 105 68 2 313 415 24 127 1.50e-13 67.8
Msa0713960 AT2G16230.1 37.374 99 59 3 317 414 353 449 1.76e-13 72.4
Msa0713960 AT2G43660.1 35.238 105 67 1 310 414 15 118 3.42e-13 66.6
Msa0713960 AT5G53600.1 35.484 93 57 2 322 414 22 111 4.03e-13 65.9
Msa0713960 AT1G78520.1 33.000 100 64 1 315 414 18 114 5.69e-13 65.5
Msa0713960 AT2G43660.2 36.957 92 57 1 323 414 29 119 6.41e-13 65.9
Msa0713960 AT1G13830.2 42.105 76 42 2 325 400 31 104 1.10e-12 67.4
Msa0713960 AT5G24318.2 42.353 85 47 2 327 411 370 452 1.22e-12 69.7
Msa0713960 AT1G13830.1 43.836 73 39 2 328 400 20 90 1.70e-12 66.6
Msa0713960 AT2G03505.4 38.750 80 47 2 329 408 21 98 6.05e-11 61.2
Msa0713960 AT2G03505.1 38.750 80 47 2 329 408 21 98 6.05e-11 61.2
Msa0713960 AT2G03505.2 38.750 80 47 2 329 408 21 98 6.05e-11 61.2
Msa0713960 AT2G03505.3 38.750 80 47 2 329 408 21 98 6.87e-11 59.7
Msa0713960 AT2G39640.1 34.343 99 62 3 314 412 345 440 7.48e-11 64.3

Find 89 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACATAAATTCTCCAAAT+TGG 0.147457 5_1:-6515510 None:intergenic
GTAGTTTGCATTGCAGTTTC+TGG 0.188078 5_1:+6513820 Msa0713960:intron
TATATTATGTGGACCCAATT+TGG 0.203652 5_1:+6515496 Msa0713960:three_prime_UTR
TCTAAATGAAAAGCATAATA+TGG 0.242719 5_1:-6515643 None:intergenic
TTGTTCTTGAGGGGCAAAAT+AGG 0.257528 5_1:-6514610 None:intergenic
CTTATGCATTCAATAGTTAC+TGG 0.260747 5_1:+6514910 Msa0713960:CDS
GAGAAGAACCTTGAGATTAT+TGG 0.281894 5_1:+6513475 Msa0713960:five_prime_UTR
CCTTGCAATGCAATAGAAAA+TGG 0.301168 5_1:+6514699 Msa0713960:CDS
GGTTGAGCAATTTCTGGACT+TGG 0.301603 5_1:-6514474 None:intergenic
GCAAAATAGGGAGAAGAAAT+TGG 0.319946 5_1:-6514597 None:intergenic
GAACAACATTAAGGTTGTTT+TGG 0.320840 5_1:-6514074 None:intergenic
CATCTTCTCTTTCTTTGCTC+TGG 0.345679 5_1:+6513533 Msa0713960:CDS
TTTGAATTTGGTACATTGTA+AGG 0.345996 5_1:-6514534 None:intergenic
CCTGCTAACTCTCCTCAAAA+TGG 0.348344 5_1:+6514663 Msa0713960:CDS
CACTTGTTTCACATAGGTTT+AGG 0.352249 5_1:-6513861 None:intergenic
ATGGTGATGGTTGTTCTTGA+GGG 0.357121 5_1:-6514620 None:intergenic
CAATGTTTATGATGTTGTTA+TGG 0.359003 5_1:-6514377 None:intergenic
GTTTGAAGAAACTCAATCAT+AGG 0.360523 5_1:-6514240 None:intergenic
TGAAGGTGGTGCTGATTGTT+TGG 0.361551 5_1:+6514830 Msa0713960:CDS
GCATGAGCAACCAATGTATC+AGG 0.366680 5_1:-6514885 None:intergenic
ACAAGAAAAGAAAATGAAAA+TGG 0.371125 5_1:+6513499 Msa0713960:exon
TGTTCTTGAGGGGCAAAATA+GGG 0.397503 5_1:-6514609 None:intergenic
TGTAACGTTGCAAGAAGCTT+TGG 0.398217 5_1:+6514794 Msa0713960:CDS
AGATCACTTTGGTTGTTGAT+TGG 0.399416 5_1:+6515435 Msa0713960:three_prime_UTR
ACAGAAGTAGAACAACATTA+AGG 0.399975 5_1:-6514083 None:intergenic
GTTGAGCAATTTCTGGACTT+GGG 0.401126 5_1:-6514473 None:intergenic
AGACATAAATTCTCCAAATT+GGG 0.404814 5_1:-6515509 None:intergenic
GGTTGGAAGTAGCTGAGTTA+AGG 0.413965 5_1:-6514197 None:intergenic
TGGCAAAAGCATAAGAGAAT+TGG 0.415770 5_1:+6514930 Msa0713960:CDS
TTCTGAACAGGTGCAATTTG+TGG 0.425677 5_1:-6514738 None:intergenic
ATGTATAAACTTATATTATG+TGG 0.426320 5_1:+6515485 Msa0713960:three_prime_UTR
TTGCACCTGTTCAGAAACTT+TGG 0.430193 5_1:+6514745 Msa0713960:CDS
TCTATCATTCCCTCAAAAGA+TGG 0.439369 5_1:+6514121 Msa0713960:CDS
GATGAAACTATTTGAGCTAA+AGG 0.452012 5_1:-6514567 None:intergenic
GTTGGAAGTAGCTGAGTTAA+GGG 0.454346 5_1:-6514196 None:intergenic
GCAATTTCTGGACTTGGGGT+TGG 0.457039 5_1:-6514468 None:intergenic
TATCAGCTGAAACTTGGCTT+AGG 0.457511 5_1:+6513974 Msa0713960:CDS
TTTGCTACTAGCTAGCTAGT+TGG 0.459447 5_1:+6515459 Msa0713960:three_prime_UTR
TTGCAGGTTTCCTTCACTGT+AGG 0.460667 5_1:+6515321 Msa0713960:intron
TTTGGTACATTGTAAGGAGC+TGG 0.460933 5_1:-6514528 None:intergenic
ATTTGAGCTAAAGGTGGTAA+AGG 0.464246 5_1:-6514558 None:intergenic
TTGGATTATGCTTGTGGTGA+AGG 0.471347 5_1:+6514813 Msa0713960:CDS
AATGGTGATGGTTGTTCTTG+AGG 0.476899 5_1:-6514621 None:intergenic
ATTGGTGGAACTTGTGACTT+TGG 0.478733 5_1:+6514948 Msa0713960:CDS
ACACACCAAAGTTTCTGAAC+AGG 0.478993 5_1:-6514750 None:intergenic
TTGTTACAATCCTGATACAT+TGG 0.480675 5_1:+6514875 Msa0713960:CDS
CTATCATTCCCTCAAAAGAT+GGG 0.481726 5_1:+6514122 Msa0713960:CDS
GTGGAACTTGTGACTTTGGT+GGG 0.482171 5_1:+6514952 Msa0713960:CDS
TGTTGCTAGAGACCTTGTTA+AGG 0.501164 5_1:-6513948 None:intergenic
ACATTGTAAGGAGCTGGATA+AGG 0.502108 5_1:-6514522 None:intergenic
GCTTCTTGCAACGTTACATC+AGG 0.504907 5_1:-6514789 None:intergenic
CAAAAGCATAAGAGAATTGG+TGG 0.510123 5_1:+6514933 Msa0713960:CDS
AAATGTGAAAACATTGAACT+TGG 0.513995 5_1:-6514288 None:intergenic
TTGAGCAATTTCTGGACTTG+GGG 0.516364 5_1:-6514472 None:intergenic
TTGTTCAAAGAAACAGCTAA+AGG 0.528055 5_1:-6513919 None:intergenic
ATCCTATGTTAAGATCACTT+TGG 0.529038 5_1:+6515424 Msa0713960:three_prime_UTR
TTCTTGTGCCAATAATCTCA+AGG 0.529385 5_1:-6513483 None:intergenic
GATACAAACTGTGGTGGTAA+TGG 0.537334 5_1:-6514639 None:intergenic
AACCAGCAGGGAAATCCAAA+GGG 0.537740 5_1:-6514497 None:intergenic
TAGGGAGAAGAAATTGGTGG+TGG 0.538044 5_1:-6514591 None:intergenic
GAACCAGCAGGGAAATCCAA+AGG 0.539386 5_1:-6514498 None:intergenic
GCTGGATAAGGAGAACCAGC+AGG 0.554077 5_1:-6514510 None:intergenic
TGTCTTCACTTGTTTCACAT+AGG 0.568580 5_1:-6513867 None:intergenic
ACAGTCCAAATCAAAAGCAA+TGG 0.570988 5_1:-6514169 None:intergenic
GTCTTGTTGGCAGAAAGTTG+AGG 0.577252 5_1:-6514052 None:intergenic
GATTATGCTTGTGGTGAAGG+TGG 0.582581 5_1:+6514816 Msa0713960:CDS
GGTGGAACTTGTGACTTTGG+TGG 0.584114 5_1:+6514951 Msa0713960:CDS
GAAGCTTTGGATTATGCTTG+TGG 0.604890 5_1:+6514807 Msa0713960:CDS
GGATTGTAACAATTCCCTTG+TGG 0.608596 5_1:-6514864 None:intergenic
AACCAAAGTGATCTTAACAT+AGG 0.609490 5_1:-6515426 None:intergenic
TTGGAGATTACTACTCCACA+AGG 0.615549 5_1:+6514849 Msa0713960:CDS
TGAGAATAAACCTACAGTGA+AGG 0.617438 5_1:-6515331 None:intergenic
AAAAGAAAATGAAAATGGTG+TGG 0.631353 5_1:+6513504 Msa0713960:exon
AACGTTACATCAGGAACACT+AGG 0.631354 5_1:-6514780 None:intergenic
TATCATTCCCTCAAAAGATG+GGG 0.632855 5_1:+6514123 Msa0713960:CDS
AACTGTGGTGGTAATGGTGA+TGG 0.632946 5_1:-6514633 None:intergenic
GAAACTATTTGAGCTAAAGG+TGG 0.639226 5_1:-6514564 None:intergenic
AAACTAATAATAGAAACCAC+AGG 0.643581 5_1:-6515382 None:intergenic
TGGTGATGGTTGTTCTTGAG+GGG 0.643729 5_1:-6514619 None:intergenic
AAATAGGGAGAAGAAATTGG+TGG 0.650191 5_1:-6514594 None:intergenic
GCAATTTGTGGATAATGTGA+AGG 0.661579 5_1:-6514726 None:intergenic
TGCATGAAGAGTTGTTCCTG+TGG 0.663059 5_1:+6515366 Msa0713960:three_prime_UTR
GAACAATGAAGACCTTAACA+AGG 0.665682 5_1:+6513936 Msa0713960:CDS
CTGGATAAGGAGAACCAGCA+GGG 0.668605 5_1:-6514509 None:intergenic
GCAGGAGATACAAACTGTGG+TGG 0.675527 5_1:-6514645 None:intergenic
ACCAGCAGGGAAATCCAAAG+GGG 0.725497 5_1:-6514496 None:intergenic
TGGAGATTACTACTCCACAA+GGG 0.734669 5_1:+6514850 Msa0713960:CDS
CCAGCAGGGAAATCCAAAGG+GGG 0.739134 5_1:-6514495 None:intergenic
TTAGCAGGAGATACAAACTG+TGG 0.759496 5_1:-6514648 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTATAAACTTATATTATG+TGG + chr5_1:6515485-6515504 Msa0713960:three_prime_UTR 15.0%
!!! ATGTCTTAATTTTTGTTATT+AGG + chr5_1:6515526-6515545 Msa0713960:three_prime_UTR 15.0%
!!! TAATTTTTGTTATTAGGATT+TGG + chr5_1:6515532-6515551 Msa0713960:three_prime_UTR 15.0%
!! ATGTATAAACTTATATTATG+TGG + chr5_1:6515485-6515504 Msa0713960:three_prime_UTR 15.0%
!!! ATGTCTTAATTTTTGTTATT+AGG + chr5_1:6515526-6515545 Msa0713960:three_prime_UTR 15.0%
!!! TAATTTTTGTTATTAGGATT+TGG + chr5_1:6515532-6515551 Msa0713960:three_prime_UTR 15.0%
!! ACAAGAAAAGAAAATGAAAA+TGG + chr5_1:6513499-6513518 Msa0713960:exon 20.0%
!!! GTTTTAACATTTTCAAATCA+TGG - chr5_1:6514221-6514240 None:intergenic 20.0%
!! TAAATTAATAAGCACTTACT+TGG - chr5_1:6515002-6515021 None:intergenic 20.0%
!! AAGTAAGTGCTTATTAATTT+AGG + chr5_1:6515001-6515020 Msa0713960:intron 20.0%
!! TGTTAATTAATAATGAGTGA+TGG + chr5_1:6515052-6515071 Msa0713960:intron 20.0%
!!! AATTAATTAGCTGTTTTAGA+TGG - chr5_1:6515236-6515255 None:intergenic 20.0%
!! TTACATTAATAATGGTGAAT+TGG + chr5_1:6515287-6515306 Msa0713960:intron 20.0%
!!! TCTAAATGAAAAGCATAATA+TGG - chr5_1:6515646-6515665 None:intergenic 20.0%
!! ACAAGAAAAGAAAATGAAAA+TGG + chr5_1:6513499-6513518 Msa0713960:exon 20.0%
!!! GTTTTAACATTTTCAAATCA+TGG - chr5_1:6514221-6514240 None:intergenic 20.0%
!! TAAATTAATAAGCACTTACT+TGG - chr5_1:6515002-6515021 None:intergenic 20.0%
!! AAGTAAGTGCTTATTAATTT+AGG + chr5_1:6515001-6515020 Msa0713960:intron 20.0%
!! TGTTAATTAATAATGAGTGA+TGG + chr5_1:6515052-6515071 Msa0713960:intron 20.0%
!!! AATTAATTAGCTGTTTTAGA+TGG - chr5_1:6515236-6515255 None:intergenic 20.0%
!! TTACATTAATAATGGTGAAT+TGG + chr5_1:6515287-6515306 Msa0713960:intron 20.0%
!!! TCTAAATGAAAAGCATAATA+TGG - chr5_1:6515646-6515665 None:intergenic 20.0%
! AAAAGAAAATGAAAATGGTG+TGG + chr5_1:6513504-6513523 Msa0713960:exon 25.0%
!! TTTTTGATGAAGGGTAATAT+GGG - chr5_1:6514011-6514030 None:intergenic 25.0%
!! ATTTTTGATGAAGGGTAATA+TGG - chr5_1:6514012-6514031 None:intergenic 25.0%
!! TAACATTTTCAAATCATGGT+TGG - chr5_1:6514217-6514236 None:intergenic 25.0%
! AAATGTGAAAACATTGAACT+TGG - chr5_1:6514291-6514310 None:intergenic 25.0%
!!! CTTTTTCATGGATTCTAAAT+GGG - chr5_1:6514343-6514362 None:intergenic 25.0%
! CAATGTTTATGATGTTGTTA+TGG - chr5_1:6514380-6514399 None:intergenic 25.0%
!! TAGTGATTTTTCTCTCTTTT+GGG - chr5_1:6514413-6514432 None:intergenic 25.0%
! TTTGAATTTGGTACATTGTA+AGG - chr5_1:6514537-6514556 None:intergenic 25.0%
! TATAGTTATTGTACATAGCA+GGG - chr5_1:6515166-6515185 None:intergenic 25.0%
! TTATAGTTATTGTACATAGC+AGG - chr5_1:6515167-6515186 None:intergenic 25.0%
! ATAAAGTATGTAACTACCAA+AGG + chr5_1:6515183-6515202 Msa0713960:intron 25.0%
! AAACTAATAATAGAAACCAC+AGG - chr5_1:6515385-6515404 None:intergenic 25.0%
! AGACATAAATTCTCCAAATT+GGG - chr5_1:6515512-6515531 None:intergenic 25.0%
! AAGACATAAATTCTCCAAAT+TGG - chr5_1:6515513-6515532 None:intergenic 25.0%
!! TCACTAATCAGAATTTTGAA+GGG - chr5_1:6515591-6515610 None:intergenic 25.0%
!! ATCACTAATCAGAATTTTGA+AGG - chr5_1:6515592-6515611 None:intergenic 25.0%
! AAAAGAAAATGAAAATGGTG+TGG + chr5_1:6513504-6513523 Msa0713960:exon 25.0%
!! TTTTTGATGAAGGGTAATAT+GGG - chr5_1:6514011-6514030 None:intergenic 25.0%
!! ATTTTTGATGAAGGGTAATA+TGG - chr5_1:6514012-6514031 None:intergenic 25.0%
!! TAACATTTTCAAATCATGGT+TGG - chr5_1:6514217-6514236 None:intergenic 25.0%
! AAATGTGAAAACATTGAACT+TGG - chr5_1:6514291-6514310 None:intergenic 25.0%
!!! CTTTTTCATGGATTCTAAAT+GGG - chr5_1:6514343-6514362 None:intergenic 25.0%
! CAATGTTTATGATGTTGTTA+TGG - chr5_1:6514380-6514399 None:intergenic 25.0%
!! TAGTGATTTTTCTCTCTTTT+GGG - chr5_1:6514413-6514432 None:intergenic 25.0%
! TTTGAATTTGGTACATTGTA+AGG - chr5_1:6514537-6514556 None:intergenic 25.0%
! TATAGTTATTGTACATAGCA+GGG - chr5_1:6515166-6515185 None:intergenic 25.0%
! TTATAGTTATTGTACATAGC+AGG - chr5_1:6515167-6515186 None:intergenic 25.0%
! ATAAAGTATGTAACTACCAA+AGG + chr5_1:6515183-6515202 Msa0713960:intron 25.0%
! AAACTAATAATAGAAACCAC+AGG - chr5_1:6515385-6515404 None:intergenic 25.0%
! AGACATAAATTCTCCAAATT+GGG - chr5_1:6515512-6515531 None:intergenic 25.0%
! AAGACATAAATTCTCCAAAT+TGG - chr5_1:6515513-6515532 None:intergenic 25.0%
!! TCACTAATCAGAATTTTGAA+GGG - chr5_1:6515591-6515610 None:intergenic 25.0%
!! ATCACTAATCAGAATTTTGA+AGG - chr5_1:6515592-6515611 None:intergenic 25.0%
TTGTTCAAAGAAACAGCTAA+AGG - chr5_1:6513922-6513941 None:intergenic 30.0%
! ACATTTTATCAGCTGAAACT+TGG + chr5_1:6513968-6513987 Msa0713960:CDS 30.0%
!!! TGTTGGTGATTTTTGATGAA+GGG - chr5_1:6514020-6514039 None:intergenic 30.0%
!!! ATGTTGGTGATTTTTGATGA+AGG - chr5_1:6514021-6514040 None:intergenic 30.0%
TCAAAAATCACCAACATTGT+TGG + chr5_1:6514024-6514043 Msa0713960:CDS 30.0%
! GAACAACATTAAGGTTGTTT+TGG - chr5_1:6514077-6514096 None:intergenic 30.0%
ACAGAAGTAGAACAACATTA+AGG - chr5_1:6514086-6514105 None:intergenic 30.0%
!! AGTTTCCATTGCTTTTGATT+TGG + chr5_1:6514164-6514183 Msa0713960:CDS 30.0%
GTTTGAAGAAACTCAATCAT+AGG - chr5_1:6514243-6514262 None:intergenic 30.0%
!!! GCTTTTTCATGGATTCTAAA+TGG - chr5_1:6514344-6514363 None:intergenic 30.0%
TTAGAATCCATGAAAAAGCT+TGG + chr5_1:6514345-6514364 Msa0713960:CDS 30.0%
!!! GAAAAATCCAAGCTTTTTCA+TGG - chr5_1:6514355-6514374 None:intergenic 30.0%
!!! TTTTCTCTCTTTTGGGATAA+TGG - chr5_1:6514406-6514425 None:intergenic 30.0%
! GTAGTGATTTTTCTCTCTTT+TGG - chr5_1:6514414-6514433 None:intergenic 30.0%
GATGAAACTATTTGAGCTAA+AGG - chr5_1:6514570-6514589 None:intergenic 30.0%
TTGTTACAATCCTGATACAT+TGG + chr5_1:6514875-6514894 Msa0713960:CDS 30.0%
CTTATGCATTCAATAGTTAC+TGG + chr5_1:6514910-6514929 Msa0713960:CDS 30.0%
! AGTTAGTTACACAGTTTGTT+AGG - chr5_1:6515209-6515228 None:intergenic 30.0%
!! ATTGGTTATTGTTTTGTTGC+AGG + chr5_1:6515305-6515324 Msa0713960:intron 30.0%
ATCCTATGTTAAGATCACTT+TGG + chr5_1:6515424-6515443 Msa0713960:three_prime_UTR 30.0%
AACCAAAGTGATCTTAACAT+AGG - chr5_1:6515429-6515448 None:intergenic 30.0%
TATATTATGTGGACCCAATT+TGG + chr5_1:6515496-6515515 Msa0713960:three_prime_UTR 30.0%
TTGTTCAAAGAAACAGCTAA+AGG - chr5_1:6513922-6513941 None:intergenic 30.0%
! ACATTTTATCAGCTGAAACT+TGG + chr5_1:6513968-6513987 Msa0713960:CDS 30.0%
!!! TGTTGGTGATTTTTGATGAA+GGG - chr5_1:6514020-6514039 None:intergenic 30.0%
!!! ATGTTGGTGATTTTTGATGA+AGG - chr5_1:6514021-6514040 None:intergenic 30.0%
TCAAAAATCACCAACATTGT+TGG + chr5_1:6514024-6514043 Msa0713960:CDS 30.0%
! GAACAACATTAAGGTTGTTT+TGG - chr5_1:6514077-6514096 None:intergenic 30.0%
ACAGAAGTAGAACAACATTA+AGG - chr5_1:6514086-6514105 None:intergenic 30.0%
!! AGTTTCCATTGCTTTTGATT+TGG + chr5_1:6514164-6514183 Msa0713960:CDS 30.0%
GTTTGAAGAAACTCAATCAT+AGG - chr5_1:6514243-6514262 None:intergenic 30.0%
!!! GCTTTTTCATGGATTCTAAA+TGG - chr5_1:6514344-6514363 None:intergenic 30.0%
TTAGAATCCATGAAAAAGCT+TGG + chr5_1:6514345-6514364 Msa0713960:CDS 30.0%
!!! GAAAAATCCAAGCTTTTTCA+TGG - chr5_1:6514355-6514374 None:intergenic 30.0%
!!! TTTTCTCTCTTTTGGGATAA+TGG - chr5_1:6514406-6514425 None:intergenic 30.0%
! GTAGTGATTTTTCTCTCTTT+TGG - chr5_1:6514414-6514433 None:intergenic 30.0%
GATGAAACTATTTGAGCTAA+AGG - chr5_1:6514570-6514589 None:intergenic 30.0%
TTGTTACAATCCTGATACAT+TGG + chr5_1:6514875-6514894 Msa0713960:CDS 30.0%
CTTATGCATTCAATAGTTAC+TGG + chr5_1:6514910-6514929 Msa0713960:CDS 30.0%
! AGTTAGTTACACAGTTTGTT+AGG - chr5_1:6515209-6515228 None:intergenic 30.0%
!! ATTGGTTATTGTTTTGTTGC+AGG + chr5_1:6515305-6515324 Msa0713960:intron 30.0%
ATCCTATGTTAAGATCACTT+TGG + chr5_1:6515424-6515443 Msa0713960:three_prime_UTR 30.0%
AACCAAAGTGATCTTAACAT+AGG - chr5_1:6515429-6515448 None:intergenic 30.0%
TATATTATGTGGACCCAATT+TGG + chr5_1:6515496-6515515 Msa0713960:three_prime_UTR 30.0%
GAGAAGAACCTTGAGATTAT+TGG + chr5_1:6513475-6513494 Msa0713960:five_prime_UTR 35.0%
TTCTTGTGCCAATAATCTCA+AGG - chr5_1:6513486-6513505 None:intergenic 35.0%
CACTTGTTTCACATAGGTTT+AGG - chr5_1:6513864-6513883 None:intergenic 35.0%
TGTCTTCACTTGTTTCACAT+AGG - chr5_1:6513870-6513889 None:intergenic 35.0%
GAACAATGAAGACCTTAACA+AGG + chr5_1:6513936-6513955 Msa0713960:CDS 35.0%
! TGAGGTTTTTCCAACAATGT+TGG - chr5_1:6514037-6514056 None:intergenic 35.0%
!!! TAAGGTTGTTTTGGTCTTGT+TGG - chr5_1:6514068-6514087 None:intergenic 35.0%
TCTATCATTCCCTCAAAAGA+TGG + chr5_1:6514121-6514140 Msa0713960:CDS 35.0%
CTATCATTCCCTCAAAAGAT+GGG + chr5_1:6514122-6514141 Msa0713960:CDS 35.0%
TATCATTCCCTCAAAAGATG+GGG + chr5_1:6514123-6514142 Msa0713960:CDS 35.0%
! TTCTAAACCCCATCTTTTGA+GGG - chr5_1:6514133-6514152 None:intergenic 35.0%
ACAGTCCAAATCAAAAGCAA+TGG - chr5_1:6514172-6514191 None:intergenic 35.0%
!!! GGTTGGTTTTATAGGAATTG+TGG - chr5_1:6514454-6514473 None:intergenic 35.0%
!!! GGTGGTAAAGGTTTTGAATT+TGG - chr5_1:6514549-6514568 None:intergenic 35.0%
ATTTGAGCTAAAGGTGGTAA+AGG - chr5_1:6514561-6514580 None:intergenic 35.0%
GAAACTATTTGAGCTAAAGG+TGG - chr5_1:6514567-6514586 None:intergenic 35.0%
AAATAGGGAGAAGAAATTGG+TGG - chr5_1:6514597-6514616 None:intergenic 35.0%
GCAAAATAGGGAGAAGAAAT+TGG - chr5_1:6514600-6514619 None:intergenic 35.0%
! TTTTCTATTGCATTGCAAGG+TGG - chr5_1:6514699-6514718 None:intergenic 35.0%
! CCATTTTCTATTGCATTGCA+AGG - chr5_1:6514702-6514721 None:intergenic 35.0%
CCTTGCAATGCAATAGAAAA+TGG + chr5_1:6514699-6514718 Msa0713960:CDS 35.0%
GCAATTTGTGGATAATGTGA+AGG - chr5_1:6514729-6514748 None:intergenic 35.0%
! TGGCAAAAGCATAAGAGAAT+TGG + chr5_1:6514930-6514949 Msa0713960:CDS 35.0%
! CAAAAGCATAAGAGAATTGG+TGG + chr5_1:6514933-6514952 Msa0713960:CDS 35.0%
TGAGAATAAACCTACAGTGA+AGG - chr5_1:6515334-6515353 None:intergenic 35.0%
AGATCACTTTGGTTGTTGAT+TGG + chr5_1:6515435-6515454 Msa0713960:three_prime_UTR 35.0%
GAGAAGAACCTTGAGATTAT+TGG + chr5_1:6513475-6513494 Msa0713960:five_prime_UTR 35.0%
TTCTTGTGCCAATAATCTCA+AGG - chr5_1:6513486-6513505 None:intergenic 35.0%
CACTTGTTTCACATAGGTTT+AGG - chr5_1:6513864-6513883 None:intergenic 35.0%
TGTCTTCACTTGTTTCACAT+AGG - chr5_1:6513870-6513889 None:intergenic 35.0%
GAACAATGAAGACCTTAACA+AGG + chr5_1:6513936-6513955 Msa0713960:CDS 35.0%
! TGAGGTTTTTCCAACAATGT+TGG - chr5_1:6514037-6514056 None:intergenic 35.0%
!!! TAAGGTTGTTTTGGTCTTGT+TGG - chr5_1:6514068-6514087 None:intergenic 35.0%
TCTATCATTCCCTCAAAAGA+TGG + chr5_1:6514121-6514140 Msa0713960:CDS 35.0%
CTATCATTCCCTCAAAAGAT+GGG + chr5_1:6514122-6514141 Msa0713960:CDS 35.0%
TATCATTCCCTCAAAAGATG+GGG + chr5_1:6514123-6514142 Msa0713960:CDS 35.0%
! TTCTAAACCCCATCTTTTGA+GGG - chr5_1:6514133-6514152 None:intergenic 35.0%
ACAGTCCAAATCAAAAGCAA+TGG - chr5_1:6514172-6514191 None:intergenic 35.0%
!!! GGTTGGTTTTATAGGAATTG+TGG - chr5_1:6514454-6514473 None:intergenic 35.0%
!!! GGTGGTAAAGGTTTTGAATT+TGG - chr5_1:6514549-6514568 None:intergenic 35.0%
ATTTGAGCTAAAGGTGGTAA+AGG - chr5_1:6514561-6514580 None:intergenic 35.0%
GAAACTATTTGAGCTAAAGG+TGG - chr5_1:6514567-6514586 None:intergenic 35.0%
AAATAGGGAGAAGAAATTGG+TGG - chr5_1:6514597-6514616 None:intergenic 35.0%
GCAAAATAGGGAGAAGAAAT+TGG - chr5_1:6514600-6514619 None:intergenic 35.0%
! TTTTCTATTGCATTGCAAGG+TGG - chr5_1:6514699-6514718 None:intergenic 35.0%
! CCATTTTCTATTGCATTGCA+AGG - chr5_1:6514702-6514721 None:intergenic 35.0%
CCTTGCAATGCAATAGAAAA+TGG + chr5_1:6514699-6514718 Msa0713960:CDS 35.0%
GCAATTTGTGGATAATGTGA+AGG - chr5_1:6514729-6514748 None:intergenic 35.0%
! TGGCAAAAGCATAAGAGAAT+TGG + chr5_1:6514930-6514949 Msa0713960:CDS 35.0%
! CAAAAGCATAAGAGAATTGG+TGG + chr5_1:6514933-6514952 Msa0713960:CDS 35.0%
TGAGAATAAACCTACAGTGA+AGG - chr5_1:6515334-6515353 None:intergenic 35.0%
AGATCACTTTGGTTGTTGAT+TGG + chr5_1:6515435-6515454 Msa0713960:three_prime_UTR 35.0%
CATCTTCTCTTTCTTTGCTC+TGG + chr5_1:6513533-6513552 Msa0713960:CDS 40.0%
! GTAGTTTGCATTGCAGTTTC+TGG + chr5_1:6513820-6513839 Msa0713960:intron 40.0%
TGTTGCTAGAGACCTTGTTA+AGG - chr5_1:6513951-6513970 None:intergenic 40.0%
TATCAGCTGAAACTTGGCTT+AGG + chr5_1:6513974-6513993 Msa0713960:CDS 40.0%
!! GTTCTAAACCCCATCTTTTG+AGG - chr5_1:6514134-6514153 None:intergenic 40.0%
GTTGGAAGTAGCTGAGTTAA+GGG - chr5_1:6514199-6514218 None:intergenic 40.0%
! TTGAGCAATTTCTGGACTTG+GGG - chr5_1:6514475-6514494 None:intergenic 40.0%
! GTTGAGCAATTTCTGGACTT+GGG - chr5_1:6514476-6514495 None:intergenic 40.0%
ACATTGTAAGGAGCTGGATA+AGG - chr5_1:6514525-6514544 None:intergenic 40.0%
TTTGGTACATTGTAAGGAGC+TGG - chr5_1:6514531-6514550 None:intergenic 40.0%
TGTTCTTGAGGGGCAAAATA+GGG - chr5_1:6514612-6514631 None:intergenic 40.0%
TTGTTCTTGAGGGGCAAAAT+AGG - chr5_1:6514613-6514632 None:intergenic 40.0%
!! ATGGTGATGGTTGTTCTTGA+GGG - chr5_1:6514623-6514642 None:intergenic 40.0%
!! AATGGTGATGGTTGTTCTTG+AGG - chr5_1:6514624-6514643 None:intergenic 40.0%
GATACAAACTGTGGTGGTAA+TGG - chr5_1:6514642-6514661 None:intergenic 40.0%
TTAGCAGGAGATACAAACTG+TGG - chr5_1:6514651-6514670 None:intergenic 40.0%
! GGACAAGTGAAACCATTTTG+AGG - chr5_1:6514678-6514697 None:intergenic 40.0%
TTCTGAACAGGTGCAATTTG+TGG - chr5_1:6514741-6514760 None:intergenic 40.0%
TTGCACCTGTTCAGAAACTT+TGG + chr5_1:6514745-6514764 Msa0713960:CDS 40.0%
ACACACCAAAGTTTCTGAAC+AGG - chr5_1:6514753-6514772 None:intergenic 40.0%
AACGTTACATCAGGAACACT+AGG - chr5_1:6514783-6514802 None:intergenic 40.0%
TGTAACGTTGCAAGAAGCTT+TGG + chr5_1:6514794-6514813 Msa0713960:CDS 40.0%
!! GAAGCTTTGGATTATGCTTG+TGG + chr5_1:6514807-6514826 Msa0713960:CDS 40.0%
TTGGATTATGCTTGTGGTGA+AGG + chr5_1:6514813-6514832 Msa0713960:CDS 40.0%
TTGGAGATTACTACTCCACA+AGG + chr5_1:6514849-6514868 Msa0713960:CDS 40.0%
TGGAGATTACTACTCCACAA+GGG + chr5_1:6514850-6514869 Msa0713960:CDS 40.0%
GGATTGTAACAATTCCCTTG+TGG - chr5_1:6514867-6514886 None:intergenic 40.0%
ATTGGTGGAACTTGTGACTT+TGG + chr5_1:6514948-6514967 Msa0713960:CDS 40.0%
AGTGATGGTTGTGTTGTGAA+TGG + chr5_1:6515067-6515086 Msa0713960:intron 40.0%
!! TACACAGTTTGTTAGGCCTT+TGG - chr5_1:6515202-6515221 None:intergenic 40.0%
TTTGCTACTAGCTAGCTAGT+TGG + chr5_1:6515459-6515478 Msa0713960:three_prime_UTR 40.0%
CATCTTCTCTTTCTTTGCTC+TGG + chr5_1:6513533-6513552 Msa0713960:CDS 40.0%
! GTAGTTTGCATTGCAGTTTC+TGG + chr5_1:6513820-6513839 Msa0713960:intron 40.0%
TGTTGCTAGAGACCTTGTTA+AGG - chr5_1:6513951-6513970 None:intergenic 40.0%
TATCAGCTGAAACTTGGCTT+AGG + chr5_1:6513974-6513993 Msa0713960:CDS 40.0%
!! GTTCTAAACCCCATCTTTTG+AGG - chr5_1:6514134-6514153 None:intergenic 40.0%
GTTGGAAGTAGCTGAGTTAA+GGG - chr5_1:6514199-6514218 None:intergenic 40.0%
! TTGAGCAATTTCTGGACTTG+GGG - chr5_1:6514475-6514494 None:intergenic 40.0%
! GTTGAGCAATTTCTGGACTT+GGG - chr5_1:6514476-6514495 None:intergenic 40.0%
ACATTGTAAGGAGCTGGATA+AGG - chr5_1:6514525-6514544 None:intergenic 40.0%
TTTGGTACATTGTAAGGAGC+TGG - chr5_1:6514531-6514550 None:intergenic 40.0%
TGTTCTTGAGGGGCAAAATA+GGG - chr5_1:6514612-6514631 None:intergenic 40.0%
TTGTTCTTGAGGGGCAAAAT+AGG - chr5_1:6514613-6514632 None:intergenic 40.0%
!! ATGGTGATGGTTGTTCTTGA+GGG - chr5_1:6514623-6514642 None:intergenic 40.0%
!! AATGGTGATGGTTGTTCTTG+AGG - chr5_1:6514624-6514643 None:intergenic 40.0%
GATACAAACTGTGGTGGTAA+TGG - chr5_1:6514642-6514661 None:intergenic 40.0%
TTAGCAGGAGATACAAACTG+TGG - chr5_1:6514651-6514670 None:intergenic 40.0%
! GGACAAGTGAAACCATTTTG+AGG - chr5_1:6514678-6514697 None:intergenic 40.0%
TTCTGAACAGGTGCAATTTG+TGG - chr5_1:6514741-6514760 None:intergenic 40.0%
TTGCACCTGTTCAGAAACTT+TGG + chr5_1:6514745-6514764 Msa0713960:CDS 40.0%
ACACACCAAAGTTTCTGAAC+AGG - chr5_1:6514753-6514772 None:intergenic 40.0%
AACGTTACATCAGGAACACT+AGG - chr5_1:6514783-6514802 None:intergenic 40.0%
TGTAACGTTGCAAGAAGCTT+TGG + chr5_1:6514794-6514813 Msa0713960:CDS 40.0%
!! GAAGCTTTGGATTATGCTTG+TGG + chr5_1:6514807-6514826 Msa0713960:CDS 40.0%
TTGGATTATGCTTGTGGTGA+AGG + chr5_1:6514813-6514832 Msa0713960:CDS 40.0%
TTGGAGATTACTACTCCACA+AGG + chr5_1:6514849-6514868 Msa0713960:CDS 40.0%
TGGAGATTACTACTCCACAA+GGG + chr5_1:6514850-6514869 Msa0713960:CDS 40.0%
GGATTGTAACAATTCCCTTG+TGG - chr5_1:6514867-6514886 None:intergenic 40.0%
ATTGGTGGAACTTGTGACTT+TGG + chr5_1:6514948-6514967 Msa0713960:CDS 40.0%
AGTGATGGTTGTGTTGTGAA+TGG + chr5_1:6515067-6515086 Msa0713960:intron 40.0%
!! TACACAGTTTGTTAGGCCTT+TGG - chr5_1:6515202-6515221 None:intergenic 40.0%
TTTGCTACTAGCTAGCTAGT+TGG + chr5_1:6515459-6515478 Msa0713960:three_prime_UTR 40.0%
! GTCTTGTTGGCAGAAAGTTG+AGG - chr5_1:6514055-6514074 None:intergenic 45.0%
GGTTGGAAGTAGCTGAGTTA+AGG - chr5_1:6514200-6514219 None:intergenic 45.0%
!!! GGACTTGGGGTTGGTTTTAT+AGG - chr5_1:6514462-6514481 None:intergenic 45.0%
! GGTTGAGCAATTTCTGGACT+TGG - chr5_1:6514477-6514496 None:intergenic 45.0%
AAAGGGGGTTGAGCAATTTC+TGG - chr5_1:6514483-6514502 None:intergenic 45.0%
AGAAATTGCTCAACCCCCTT+TGG + chr5_1:6514482-6514501 Msa0713960:CDS 45.0%
AACCAGCAGGGAAATCCAAA+GGG - chr5_1:6514500-6514519 None:intergenic 45.0%
TAGGGAGAAGAAATTGGTGG+TGG - chr5_1:6514594-6514613 None:intergenic 45.0%
!! TGGTGATGGTTGTTCTTGAG+GGG - chr5_1:6514622-6514641 None:intergenic 45.0%
!! AACTGTGGTGGTAATGGTGA+TGG - chr5_1:6514636-6514655 None:intergenic 45.0%
!! CCATTTTGAGGAGAGTTAGC+AGG - chr5_1:6514666-6514685 None:intergenic 45.0%
CCTGCTAACTCTCCTCAAAA+TGG + chr5_1:6514663-6514682 Msa0713960:CDS 45.0%
GCTTCTTGCAACGTTACATC+AGG - chr5_1:6514792-6514811 None:intergenic 45.0%
GATTATGCTTGTGGTGAAGG+TGG + chr5_1:6514816-6514835 Msa0713960:CDS 45.0%
!! TGAAGGTGGTGCTGATTGTT+TGG + chr5_1:6514830-6514849 Msa0713960:CDS 45.0%
GCATGAGCAACCAATGTATC+AGG - chr5_1:6514888-6514907 None:intergenic 45.0%
GTGGAACTTGTGACTTTGGT+GGG + chr5_1:6514952-6514971 Msa0713960:CDS 45.0%
TTGCAGGTTTCCTTCACTGT+AGG + chr5_1:6515321-6515340 Msa0713960:intron 45.0%
TGCATGAAGAGTTGTTCCTG+TGG + chr5_1:6515366-6515385 Msa0713960:three_prime_UTR 45.0%
! GTCTTGTTGGCAGAAAGTTG+AGG - chr5_1:6514055-6514074 None:intergenic 45.0%
GGTTGGAAGTAGCTGAGTTA+AGG - chr5_1:6514200-6514219 None:intergenic 45.0%
!!! GGACTTGGGGTTGGTTTTAT+AGG - chr5_1:6514462-6514481 None:intergenic 45.0%
! GGTTGAGCAATTTCTGGACT+TGG - chr5_1:6514477-6514496 None:intergenic 45.0%
AAAGGGGGTTGAGCAATTTC+TGG - chr5_1:6514483-6514502 None:intergenic 45.0%
AGAAATTGCTCAACCCCCTT+TGG + chr5_1:6514482-6514501 Msa0713960:CDS 45.0%
AACCAGCAGGGAAATCCAAA+GGG - chr5_1:6514500-6514519 None:intergenic 45.0%
TAGGGAGAAGAAATTGGTGG+TGG - chr5_1:6514594-6514613 None:intergenic 45.0%
!! TGGTGATGGTTGTTCTTGAG+GGG - chr5_1:6514622-6514641 None:intergenic 45.0%
!! AACTGTGGTGGTAATGGTGA+TGG - chr5_1:6514636-6514655 None:intergenic 45.0%
!! CCATTTTGAGGAGAGTTAGC+AGG - chr5_1:6514666-6514685 None:intergenic 45.0%
CCTGCTAACTCTCCTCAAAA+TGG + chr5_1:6514663-6514682 Msa0713960:CDS 45.0%
GCTTCTTGCAACGTTACATC+AGG - chr5_1:6514792-6514811 None:intergenic 45.0%
GATTATGCTTGTGGTGAAGG+TGG + chr5_1:6514816-6514835 Msa0713960:CDS 45.0%
!! TGAAGGTGGTGCTGATTGTT+TGG + chr5_1:6514830-6514849 Msa0713960:CDS 45.0%
GCATGAGCAACCAATGTATC+AGG - chr5_1:6514888-6514907 None:intergenic 45.0%
GTGGAACTTGTGACTTTGGT+GGG + chr5_1:6514952-6514971 Msa0713960:CDS 45.0%
TTGCAGGTTTCCTTCACTGT+AGG + chr5_1:6515321-6515340 Msa0713960:intron 45.0%
TGCATGAAGAGTTGTTCCTG+TGG + chr5_1:6515366-6515385 Msa0713960:three_prime_UTR 45.0%
!! TTATTTATTTACATTAATAA+TGG + chr5_1:6515279-6515298 Msa0713960:intron 5.0%
!! TTATTTATTTACATTAATAA+TGG + chr5_1:6515279-6515298 Msa0713960:intron 5.0%
! GCAATTTCTGGACTTGGGGT+TGG - chr5_1:6514471-6514490 None:intergenic 50.0%
ACCAGCAGGGAAATCCAAAG+GGG - chr5_1:6514499-6514518 None:intergenic 50.0%
GAACCAGCAGGGAAATCCAA+AGG - chr5_1:6514501-6514520 None:intergenic 50.0%
CTGGATAAGGAGAACCAGCA+GGG - chr5_1:6514512-6514531 None:intergenic 50.0%
GCAGGAGATACAAACTGTGG+TGG - chr5_1:6514648-6514667 None:intergenic 50.0%
GGTGGAACTTGTGACTTTGG+TGG + chr5_1:6514951-6514970 Msa0713960:CDS 50.0%
! GCAATTTCTGGACTTGGGGT+TGG - chr5_1:6514471-6514490 None:intergenic 50.0%
ACCAGCAGGGAAATCCAAAG+GGG - chr5_1:6514499-6514518 None:intergenic 50.0%
GAACCAGCAGGGAAATCCAA+AGG - chr5_1:6514501-6514520 None:intergenic 50.0%
CTGGATAAGGAGAACCAGCA+GGG - chr5_1:6514512-6514531 None:intergenic 50.0%
GCAGGAGATACAAACTGTGG+TGG - chr5_1:6514648-6514667 None:intergenic 50.0%
GGTGGAACTTGTGACTTTGG+TGG + chr5_1:6514951-6514970 Msa0713960:CDS 50.0%
CCAGCAGGGAAATCCAAAGG+GGG - chr5_1:6514498-6514517 None:intergenic 55.0%
GCTGGATAAGGAGAACCAGC+AGG - chr5_1:6514513-6514532 None:intergenic 55.0%
CCAGCAGGGAAATCCAAAGG+GGG - chr5_1:6514498-6514517 None:intergenic 55.0%
GCTGGATAAGGAGAACCAGC+AGG - chr5_1:6514513-6514532 None:intergenic 55.0%
CCCCCTTTGGATTTCCCTGC+TGG + chr5_1:6514495-6514514 Msa0713960:CDS 60.0%
CCCCCTTTGGATTTCCCTGC+TGG + chr5_1:6514495-6514514 Msa0713960:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 6513448 6515671 6513448 ID=Msa0713960;Name=Msa0713960
chr5_1 mRNA 6513448 6515671 6513448 ID=Msa0713960-mRNA-1;Parent=Msa0713960;Name=Msa0713960-mRNA-1;_AED=0.01;_eAED=0.01;_QI=64|1|1|1|1|1|3|313|417
chr5_1 exon 6513448 6513554 6513448 ID=Msa0713960-mRNA-1:exon:2713;Parent=Msa0713960-mRNA-1
chr5_1 exon 6513824 6515002 6513824 ID=Msa0713960-mRNA-1:exon:2714;Parent=Msa0713960-mRNA-1
chr5_1 exon 6515327 6515671 6515327 ID=Msa0713960-mRNA-1:exon:2715;Parent=Msa0713960-mRNA-1
chr5_1 five_prime_UTR 6513448 6513511 6513448 ID=Msa0713960-mRNA-1:five_prime_utr;Parent=Msa0713960-mRNA-1
chr5_1 CDS 6513512 6513554 6513512 ID=Msa0713960-mRNA-1:cds;Parent=Msa0713960-mRNA-1
chr5_1 CDS 6513824 6515002 6513824 ID=Msa0713960-mRNA-1:cds;Parent=Msa0713960-mRNA-1
chr5_1 CDS 6515327 6515358 6515327 ID=Msa0713960-mRNA-1:cds;Parent=Msa0713960-mRNA-1
chr5_1 three_prime_UTR 6515359 6515671 6515359 ID=Msa0713960-mRNA-1:three_prime_utr;Parent=Msa0713960-mRNA-1
Gene Sequence

>Msa0713960

ATGAAAATGGTGTGGCTATTTCATCTTCTCTTTCTTTGCTCTGTTTGCATTGCAGTTTCTGGTCAACAAAACATTGAGTTCCTAAACCTATGTGAAACAAGTGAAGACATTGCAACATCTTCATCTCACAGTGAGTTTCCTTTAGCTGTTTCTTTGAACAATGAAGACCTTAACAAGGTCTCTAGCAACATTTTATCAGCTGAAACTTGGCTTAGGTTAAACATTTTCCCATATTACCCTTCATCAAAAATCACCAACATTGTTGGAAAAACCTCAACTTTCTGCCAACAAGACCAAAACAACCTTAATGTTGTTCTACTTCTGTCTTCTTTGAAAAATCTCTATCATTCCCTCAAAAGATGGGGTTTAGAACAAGACATCAAAGTTTCCATTGCTTTTGATTTGGACTGTTTTACCCTTAACTCAGCTACTTCCAACCATGATTTGAAAATGTTAAAACCTATGATTGAGTTTCTTCAAACTGTTAACTCAACATTTTCTCTCATACCAAGTTCAATGTTTTCACATTTTTCTGATAAAAGTTTAAACTTTATGTCTTCCCATTTAGAATCCATGAAAAAGCTTGGATTTTTCAACCATAACAACATCATAAACATTGCTACCATTATCCCAAAAGAGAGAAAAATCACTACTAGAAAACTTTCAGTTACCACAATTCCTATAAAACCAACCCCAAGTCCAGAAATTGCTCAACCCCCTTTGGATTTCCCTGCTGGTTCTCCTTATCCAGCTCCTTACAATGTACCAAATTCAAAACCTTTACCACCTTTAGCTCAAATAGTTTCATCTCCACCACCAATTTCTTCTCCCTATTTTGCCCCTCAAGAACAACCATCACCATTACCACCACAGTTTGTATCTCCTGCTAACTCTCCTCAAAATGGTTTCACTTGTCCACCTTGCAATGCAATAGAAAATGGTTCACCTTCACATTATCCACAAATTGCACCTGTTCAGAAACTTTGGTGTGTTGCAAAGCCTAGTGTTCCTGATGTAACGTTGCAAGAAGCTTTGGATTATGCTTGTGGTGAAGGTGGTGCTGATTGTTTGGAGATTACTACTCCACAAGGGAATTGTTACAATCCTGATACATTGGTTGCTCATGCTTCTTATGCATTCAATAGTTACTGGCAAAAGCATAAGAGAATTGGTGGAACTTGTGACTTTGGTGGGACTGCTATGTTGATTCATTCTGATCCAAGTTTCCTTCACTGTAGGTTTATTCTCAGCTAG
Protein sequence

>Msa0713960

MKMVWLFHLLFLCSVCIAVSGQQNIEFLNLCETSEDIATSSSHSEFPLAVSLNNEDLNKVSSNILSAETWLRLNIFPYYPSSKITNIVGKTSTFCQQDQNNLNVVLLLSSLKNLYHSLKRWGLEQDIKVSIAFDLDCFTLNSATSNHDLKMLKPMIEFLQTVNSTFSLIPSSMFSHFSDKSLNFMSSHLESMKKLGFFNHNNIINIATIIPKERKITTRKLSVTTIPIKPTPSPEIAQPPLDFPAGSPYPAPYNVPNSKPLPPLAQIVSSPPPISSPYFAPQEQPSPLPPQFVSPANSPQNGFTCPPCNAIENGSPSHYPQIAPVQKLWCVAKPSVPDVTLQEALDYACGEGGADCLEITTPQGNCYNPDTLVAHASYAFNSYWQKHKRIGGTCDFGGTAMLIHSDPSFLHCRFILS*