AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0835350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0835350 MtrunA17_Chr5g0401301 95.943 419 15 1 1 419 1 417 0.0 812
Msa0835350 MtrunA17_Chr1g0177171 27.880 434 268 12 23 416 62 490 2.17e-38 144
Msa0835350 MtrunA17_Chr3g0120401 61.538 91 33 2 326 416 152 240 3.74e-32 122
Msa0835350 MtrunA17_Chr1g0213651 60.215 93 35 2 325 417 270 360 5.08e-31 122
Msa0835350 MtrunA17_Chr7g0234821 24.480 433 262 11 24 416 51 458 4.99e-28 115
Msa0835350 MtrunA17_Chr8g0339191 25.701 428 252 15 28 416 58 458 2.25e-27 113
Msa0835350 MtrunA17_Chr5g0439251 53.191 94 37 2 331 418 382 474 1.25e-25 108
Msa0835350 MtrunA17_Chr5g0447651 54.545 88 39 1 329 416 28 114 1.15e-24 98.2
Msa0835350 MtrunA17_Chr8g0378361 52.326 86 39 2 331 416 30 113 1.16e-23 97.4
Msa0835350 MtrunA17_Chr3g0096451 50.000 86 41 2 331 416 112 195 5.08e-23 97.4
Msa0835350 MtrunA17_Chr8g0378621 49.425 87 43 1 330 416 363 448 5.87e-23 100
Msa0835350 MtrunA17_Chr7g0271411 53.488 86 38 2 331 416 160 243 8.16e-23 97.8
Msa0835350 MtrunA17_Chr8g0378371 49.462 93 42 3 324 416 26 113 1.66e-22 92.0
Msa0835350 MtrunA17_Chr4g0076141 51.220 82 39 1 331 412 13 93 1.41e-21 89.0
Msa0835350 MtrunA17_Chr8g0381081 51.163 86 41 1 331 416 57 141 1.44e-21 91.3
Msa0835350 MtrunA17_Chr1g0193131 60.494 81 27 4 331 410 420 496 5.19e-21 95.5
Msa0835350 MtrunA17_Chr5g0443331 50.000 86 41 2 331 416 108 191 1.02e-20 91.3
Msa0835350 MtrunA17_Chr4g0075031 47.727 88 45 1 329 416 380 466 1.68e-20 93.6
Msa0835350 MtrunA17_Chr4g0076131 45.455 88 43 2 331 416 28 112 3.29e-20 85.5
Msa0835350 MtrunA17_Chr8g0378381 45.455 88 46 2 329 416 30 115 9.45e-20 84.3
Msa0835350 MtrunA17_Chr3g0121271 45.455 88 47 1 329 416 360 446 5.89e-19 89.0
Msa0835350 MtrunA17_Chr4g0040911 46.067 89 47 1 328 416 34 121 1.15e-18 83.2
Msa0835350 MtrunA17_Chr2g0304061 51.685 89 40 3 331 419 158 243 1.33e-18 86.3
Msa0835350 MtrunA17_Chr8g0389741 43.678 87 48 1 331 417 368 453 2.39e-18 87.0
Msa0835350 MtrunA17_Chr4g0061401 49.462 93 40 4 326 416 19 106 3.18e-18 83.2
Msa0835350 MtrunA17_Chr8g0378401 47.561 82 41 2 329 410 30 109 6.22e-18 79.3
Msa0835350 MtrunA17_Chr1g0161011 44.944 89 48 1 328 416 358 445 6.83e-18 85.5
Msa0835350 MtrunA17_Chr4g0034901 40.000 110 62 2 310 416 351 459 9.39e-18 85.1
Msa0835350 MtrunA17_Chr6g0487261 43.182 88 48 2 331 417 369 455 1.25e-17 84.7
Msa0835350 MtrunA17_Chr4g0043511 44.318 88 47 2 329 416 418 503 4.48e-17 83.2
Msa0835350 MtrunA17_Chr4g0046311 23.095 420 266 13 28 410 52 451 8.28e-17 82.0
Msa0835350 MtrunA17_Chr5g0434591 41.573 89 51 1 328 416 357 444 1.24e-16 81.6
Msa0835350 MtrunA17_Chr8g0380231 55.882 68 28 2 343 410 29 94 1.52e-16 75.1
Msa0835350 MtrunA17_Chr5g0419121 50.685 73 34 2 330 402 21 91 3.32e-16 78.2
Msa0835350 MtrunA17_Chr1g0154991 43.023 86 47 2 331 416 20 103 1.46e-15 72.4
Msa0835350 MtrunA17_Chr4g0043151 47.945 73 36 2 330 402 20 90 1.95e-15 74.3
Msa0835350 MtrunA17_Chr3g0110371 42.529 87 47 2 331 416 361 445 7.63e-15 76.3
Msa0835350 MtrunA17_Chr3g0144531 41.860 86 48 2 331 416 371 454 2.29e-14 74.7
Msa0835350 MtrunA17_Chr2g0313891 39.080 87 50 1 328 414 27 110 6.36e-13 65.1
Msa0835350 MtrunA17_Chr7g0223961 35.000 100 63 2 316 415 350 447 2.86e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0835350 AT5G67460.1 41.995 431 178 17 6 419 5 380 9.22e-82 256
Msa0835350 AT5G67460.2 41.412 425 177 17 6 413 5 374 2.04e-76 242
Msa0835350 AT5G55180.1 28.873 426 251 14 23 416 51 456 2.25e-39 147
Msa0835350 AT5G55180.2 29.048 420 246 14 23 410 51 450 1.81e-37 142
Msa0835350 AT5G56590.1 26.555 418 273 12 22 417 49 454 1.35e-31 126
Msa0835350 AT1G79480.2 58.065 93 37 2 324 416 303 393 9.18e-31 122
Msa0835350 AT1G79480.1 58.065 93 37 2 324 416 304 394 9.41e-31 122
Msa0835350 AT2G05790.1 58.140 86 35 1 331 416 384 468 2.79e-27 113
Msa0835350 AT4G26830.2 58.140 86 35 1 331 416 371 455 2.27e-26 110
Msa0835350 AT4G26830.1 58.140 86 35 1 331 416 367 451 2.62e-26 110
Msa0835350 AT2G30933.1 57.143 91 37 2 326 416 77 165 4.12e-26 105
Msa0835350 AT2G30933.2 58.824 85 33 2 326 410 77 159 8.56e-25 100
Msa0835350 AT2G30933.3 58.824 85 33 2 326 410 77 159 8.56e-25 100
Msa0835350 AT5G35740.1 48.837 86 44 0 331 416 30 115 1.88e-24 97.8
Msa0835350 AT3G07320.1 24.942 429 263 16 24 416 50 455 2.38e-23 102
Msa0835350 AT1G66855.1 44.444 90 49 1 327 416 22 110 1.29e-22 92.4
Msa0835350 AT2G04910.2 47.674 86 44 1 331 416 15 99 5.88e-22 90.5
Msa0835350 AT4G05430.1 46.512 86 45 1 331 416 23 107 3.02e-21 89.7
Msa0835350 AT2G04910.1 48.750 80 40 1 331 410 15 93 3.30e-21 89.0
Msa0835350 AT1G11820.2 48.864 88 44 1 329 416 379 465 3.39e-21 96.3
Msa0835350 AT4G05430.3 46.512 86 45 1 331 416 48 132 4.03e-21 90.1
Msa0835350 AT1G29380.3 54.023 87 36 3 331 416 148 231 6.67e-21 92.0
Msa0835350 AT4G13600.1 49.425 87 42 2 330 416 60 144 6.88e-21 91.3
Msa0835350 AT1G29380.1 54.023 87 36 3 331 416 148 231 7.70e-21 92.8
Msa0835350 AT1G66870.1 43.182 88 49 1 329 416 24 110 8.42e-21 87.4
Msa0835350 AT1G66870.2 43.182 88 49 1 329 416 51 137 1.49e-20 87.8
Msa0835350 AT1G11820.3 50.000 84 41 1 329 412 379 461 2.17e-20 93.6
Msa0835350 AT1G18650.1 51.163 86 40 2 331 416 21 104 3.21e-20 88.2
Msa0835350 AT5G61130.1 48.837 86 42 2 331 416 21 104 3.48e-20 88.6
Msa0835350 AT3G13560.4 46.667 90 47 1 330 419 361 449 3.70e-20 92.8
Msa0835350 AT3G13560.1 46.667 90 47 1 330 419 361 449 3.70e-20 92.8
Msa0835350 AT3G13560.2 46.667 90 47 1 330 419 361 449 3.70e-20 92.8
Msa0835350 AT3G13560.5 46.667 90 47 1 330 419 361 449 3.70e-20 92.8
Msa0835350 AT3G13560.3 46.667 90 47 1 330 419 361 449 3.70e-20 92.8
Msa0835350 AT4G05430.2 48.750 80 40 1 331 410 48 126 3.94e-20 86.3
Msa0835350 AT1G18650.2 51.163 86 40 2 331 416 21 104 4.65e-20 86.3
Msa0835350 AT1G29380.4 55.556 81 32 3 331 410 148 225 7.34e-20 88.2
Msa0835350 AT1G29380.2 55.556 81 32 3 331 410 148 225 7.34e-20 88.2
Msa0835350 AT2G01630.1 45.455 88 47 1 329 416 358 444 1.33e-19 91.3
Msa0835350 AT1G09460.1 51.163 86 38 3 331 416 137 218 4.02e-19 88.2
Msa0835350 AT2G01630.3 45.783 83 44 1 329 411 358 439 5.51e-19 89.0
Msa0835350 AT3G23770.1 23.810 399 249 16 54 416 92 471 1.53e-18 87.8
Msa0835350 AT5G08000.2 48.889 90 44 2 327 416 36 123 1.99e-18 84.0
Msa0835350 AT5G08000.1 50.000 86 41 2 331 416 21 104 4.11e-18 82.4
Msa0835350 AT4G29360.1 35.433 127 81 1 293 419 353 478 5.46e-18 86.7
Msa0835350 AT4G29360.1 30.464 151 95 3 24 168 52 198 7.68e-11 64.3
Msa0835350 AT4G29360.2 35.433 127 81 1 293 419 363 488 5.68e-18 86.7
Msa0835350 AT4G29360.2 30.464 151 95 3 24 168 62 208 8.28e-11 64.3
Msa0835350 AT5G63225.1 46.429 84 43 2 331 414 25 106 9.27e-18 79.0
Msa0835350 AT5G63230.1 42.045 88 49 2 329 416 24 109 1.74e-17 78.2
Msa0835350 AT1G66852.1 39.773 88 51 2 329 416 24 109 2.44e-17 77.8
Msa0835350 AT3G55430.1 39.535 86 50 2 331 416 366 449 4.37e-17 83.2
Msa0835350 AT2G43670.1 41.667 84 46 2 331 414 35 115 3.60e-16 75.1
Msa0835350 AT5G63250.1 40.541 111 56 4 309 417 27 129 5.06e-16 74.7
Msa0835350 AT4G16165.1 35.227 88 56 1 329 416 24 110 5.58e-16 73.9
Msa0835350 AT5G53610.1 45.349 86 44 2 331 416 28 110 7.09e-16 73.6
Msa0835350 AT4G09090.1 40.230 87 50 2 331 417 31 115 8.71e-16 73.6
Msa0835350 AT2G29735.1 41.111 90 49 3 329 416 29 116 1.05e-15 73.6
Msa0835350 AT4G09465.1 38.636 88 52 2 329 416 29 114 1.65e-15 72.8
Msa0835350 AT4G09462.1 38.636 88 52 2 329 416 27 112 1.68e-15 72.8
Msa0835350 AT4G09464.1 38.636 88 52 2 329 416 29 114 1.95e-15 72.8
Msa0835350 AT4G09467.1 38.636 88 52 2 329 416 29 114 1.95e-15 72.8
Msa0835350 AT4G34480.3 43.023 86 47 2 331 416 253 336 3.10e-15 77.4
Msa0835350 AT4G34480.1 43.023 86 47 2 331 416 364 447 3.23e-15 77.8
Msa0835350 AT4G09466.1 37.500 88 53 2 329 416 29 114 5.89e-15 71.2
Msa0835350 AT1G66250.1 39.326 89 53 1 328 416 365 452 1.04e-14 76.3
Msa0835350 AT2G16230.1 37.864 103 61 3 315 416 349 449 1.99e-14 75.5
Msa0835350 AT5G53600.1 36.559 93 56 2 324 416 22 111 3.33e-14 68.9
Msa0835350 AT1G69295.1 48.611 72 34 3 331 402 21 89 4.22e-14 71.6
Msa0835350 AT1G26450.1 53.226 62 27 2 343 404 32 91 5.35e-14 70.9
Msa0835350 AT3G58100.1 39.773 88 52 1 329 416 39 125 5.69e-14 70.5
Msa0835350 AT1G69295.2 48.611 72 34 3 331 402 17 85 5.73e-14 71.2
Msa0835350 AT3G58100.2 39.773 88 52 1 329 416 115 201 1.17e-13 70.9
Msa0835350 AT2G43660.2 36.957 92 57 1 325 416 29 119 2.19e-13 67.0
Msa0835350 AT2G43660.1 36.957 92 57 1 325 416 28 118 2.26e-13 67.0
Msa0835350 AT5G63240.1 32.039 103 67 2 317 417 26 127 2.28e-13 67.4
Msa0835350 AT5G24318.3 37.736 106 59 4 315 416 214 316 5.75e-13 69.7
Msa0835350 AT5G24318.1 37.736 106 59 4 315 416 353 455 5.88e-13 70.9
Msa0835350 AT1G13830.2 42.105 76 42 2 327 402 31 104 1.22e-12 67.4
Msa0835350 AT1G13830.1 43.836 73 39 2 330 402 20 90 2.07e-12 66.2
Msa0835350 AT1G78520.1 32.000 100 65 1 317 416 18 114 4.18e-12 63.2
Msa0835350 AT5G24318.2 38.835 103 56 4 315 413 353 452 6.35e-12 67.4
Msa0835350 AT3G28250.1 40.000 80 45 2 331 410 3 79 1.23e-11 62.0
Msa0835350 AT2G03505.4 38.750 80 47 2 331 410 21 98 2.31e-11 62.4
Msa0835350 AT2G03505.1 38.750 80 47 2 331 410 21 98 2.31e-11 62.4
Msa0835350 AT2G03505.2 38.750 80 47 2 331 410 21 98 2.31e-11 62.4
Msa0835350 AT2G03505.3 38.750 80 47 2 331 410 21 98 2.67e-11 60.8
Msa0835350 AT2G42930.1 37.113 97 58 3 314 410 23 116 5.13e-11 60.5

Find 92 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACATAAATTCTCCAAAT+TGG 0.147457 5_4:-8659447 None:intergenic
GTAGTTTGCATTGCAGTTTC+TGG 0.188078 5_4:+8657763 Msa0835350:intron
TATATTATGTGGACCCAATT+TGG 0.203652 5_4:+8659433 Msa0835350:three_prime_UTR
TCTAAATGAAAAGCATAATA+TGG 0.242719 5_4:-8659579 None:intergenic
TTGTTCTTGAGGGGCAAAAT+AGG 0.257528 5_4:-8658553 None:intergenic
CTTATGCATTCAATAGTTAC+TGG 0.260747 5_4:+8658859 Msa0835350:CDS
GAGAAGAACCTTGAGATTAT+TGG 0.281894 5_4:+8657518 Msa0835350:five_prime_UTR
GTTGAGCAATTTCTGGAATT+GGG 0.288063 5_4:-8658416 None:intergenic
CCTTGCAATGCAATAGAAAA+TGG 0.301168 5_4:+8658642 Msa0835350:CDS
TTTAACCTAAGCCAAGTTTC+AGG 0.305887 5_4:-8657922 None:intergenic
GGTTGAGCAATTTCTGGAAT+TGG 0.309049 5_4:-8658417 None:intergenic
GCAAAATAGGGAGAAGAAAT+TGG 0.319946 5_4:-8658540 None:intergenic
GAACAACATTAAGGTTGTTT+TGG 0.320840 5_4:-8658017 None:intergenic
CATCTTCTCTTTCTTTGCTC+TGG 0.345679 5_4:+8657576 Msa0835350:CDS
TTTGAATTTGGTACATTGTA+AGG 0.345996 5_4:-8658477 None:intergenic
CCTGCTAACTCTCCTCAAAA+TGG 0.348344 5_4:+8658606 Msa0835350:CDS
CACTTGTTTCACATAGGTTT+AGG 0.352249 5_4:-8657804 None:intergenic
ATGGTGATGGTTGTTCTTGA+GGG 0.357121 5_4:-8658563 None:intergenic
TGAAGGTGGTGCTGATTGTT+TGG 0.361551 5_4:+8658779 Msa0835350:CDS
GCATGAGCAACCAATGTATC+AGG 0.366680 5_4:-8658834 None:intergenic
ACAAGAAAAGAAAATGAAAA+TGG 0.371125 5_4:+8657542 Msa0835350:exon
GGTTTGAAGTAGCTGAGTTA+AGG 0.381419 5_4:-8658140 None:intergenic
TGAAACGTTGCAAGAAGCTT+TGG 0.385008 5_4:+8658743 Msa0835350:CDS
GCTTGAAGAAACTCAATCAT+AGG 0.387058 5_4:-8658183 None:intergenic
TGTTCTTGAGGGGCAAAATA+GGG 0.397503 5_4:-8658552 None:intergenic
AGATCACTTTGGTTGTTGAT+TGG 0.399416 5_4:+8659372 Msa0835350:three_prime_UTR
ACAGAAGTAGAACAACATTA+AGG 0.399975 5_4:-8658026 None:intergenic
AGACATAAATTCTCCAAATT+GGG 0.404814 5_4:-8659446 None:intergenic
TTTCTCTCATACCAAGTTCA+AGG 0.409782 5_4:+8658220 Msa0835350:CDS
CAATGTTTATGATATTGTTA+TGG 0.410294 5_4:-8658320 None:intergenic
GTTTGAAGTAGCTGAGTTAA+GGG 0.410988 5_4:-8658139 None:intergenic
TGGCAAAAGCATAAGAGAAT+TGG 0.415770 5_4:+8658879 Msa0835350:CDS
GCTTCTTGCAACGTTTCATC+AGG 0.416313 5_4:-8658738 None:intergenic
TTCTGAACAGGTGCAATTTG+TGG 0.425677 5_4:-8658687 None:intergenic
ATGTATAAACTTATATTATG+TGG 0.426320 5_4:+8659422 Msa0835350:three_prime_UTR
TTGCACCTGTTCAGAAACTT+TGG 0.430193 5_4:+8658694 Msa0835350:CDS
TCTATCATTCCCTCAAAAGA+TGG 0.439369 5_4:+8658064 Msa0835350:CDS
GATGAAACTATTTGAGCTAA+AGG 0.452012 5_4:-8658510 None:intergenic
TTTGCTACTAGCTAGCTAGT+TGG 0.459447 5_4:+8659396 Msa0835350:three_prime_UTR
TTGCAGGTTTCCTTCACTGT+AGG 0.460667 5_4:+8659258 Msa0835350:intron
GCAATTTCTGGAATTGGGGT+TGG 0.460853 5_4:-8658411 None:intergenic
TATCACCTGAAACTTGGCTT+AGG 0.460891 5_4:+8657917 Msa0835350:CDS
TTTGGTACATTGTAAGGAGC+TGG 0.460933 5_4:-8658471 None:intergenic
ATTTGAGCTAAAGGTGGTAA+AGG 0.464246 5_4:-8658501 None:intergenic
TTGGATTATGCTTGTGGTGA+AGG 0.471347 5_4:+8658762 Msa0835350:CDS
AATGGTGATGGTTGTTCTTG+AGG 0.476899 5_4:-8658564 None:intergenic
ATTGGTGGAACTTGTGACTT+TGG 0.478733 5_4:+8658897 Msa0835350:CDS
ACACACCAAAGTTTCTGAAC+AGG 0.478993 5_4:-8658699 None:intergenic
TTGTTACAATCCTGATACAT+TGG 0.480675 5_4:+8658824 Msa0835350:CDS
CTATCATTCCCTCAAAAGAT+GGG 0.481726 5_4:+8658065 Msa0835350:CDS
GTGGAACTTGTGACTTTGGT+GGG 0.482171 5_4:+8658901 Msa0835350:CDS
TGTTGCTAGAGACCTTGTTA+AGG 0.501164 5_4:-8657891 None:intergenic
ACATTGTAAGGAGCTGGATA+AGG 0.502108 5_4:-8658465 None:intergenic
CAAAAGCATAAGAGAATTGG+TGG 0.510123 5_4:+8658882 Msa0835350:CDS
AAATGTGAAAACCTTGAACT+TGG 0.511512 5_4:-8658231 None:intergenic
TTGAGCAATTTCTGGAATTG+GGG 0.520319 5_4:-8658415 None:intergenic
TTGTTCAAAGAAACAGCTAA+AGG 0.528055 5_4:-8657862 None:intergenic
ATCCTATGTTAAGATCACTT+TGG 0.529038 5_4:+8659361 Msa0835350:three_prime_UTR
TTCTTGTGCCAATAATCTCA+AGG 0.529385 5_4:-8657526 None:intergenic
GATACAAACTGTGGTGGTAA+TGG 0.537334 5_4:-8658582 None:intergenic
AACCAGCAGGGAAATCCAAA+GGG 0.537740 5_4:-8658440 None:intergenic
TAGGGAGAAGAAATTGGTGG+TGG 0.538044 5_4:-8658534 None:intergenic
GAACCAGCAGGGAAATCCAA+AGG 0.539386 5_4:-8658441 None:intergenic
GCTGGATAAGGAGAACCAGC+AGG 0.554077 5_4:-8658453 None:intergenic
TGTCTTCACTTGTTTCACAT+AGG 0.568580 5_4:-8657810 None:intergenic
ACAGTCCAAATCAAAAGCAA+TGG 0.570988 5_4:-8658112 None:intergenic
GTCTTGTTGGCAGAAAGTTG+AGG 0.577252 5_4:-8657995 None:intergenic
GATTATGCTTGTGGTGAAGG+TGG 0.582581 5_4:+8658765 Msa0835350:CDS
GGTGGAACTTGTGACTTTGG+TGG 0.584114 5_4:+8658900 Msa0835350:CDS
AACGTTTCATCAGGAACACT+AGG 0.602336 5_4:-8658729 None:intergenic
GAAGCTTTGGATTATGCTTG+TGG 0.604890 5_4:+8658756 Msa0835350:CDS
GGATTGTAACAATTCCCTTG+TGG 0.608596 5_4:-8658813 None:intergenic
AACCAAAGTGATCTTAACAT+AGG 0.609490 5_4:-8659363 None:intergenic
TTGGAGATTACTACTCCACA+AGG 0.615549 5_4:+8658798 Msa0835350:CDS
TGAGAATAAACCTACAGTGA+AGG 0.617438 5_4:-8659268 None:intergenic
AAAAGAAAATGAAAATGGTG+TGG 0.631353 5_4:+8657547 Msa0835350:exon
TATCATTCCCTCAAAAGATG+GGG 0.632855 5_4:+8658066 Msa0835350:CDS
AACTGTGGTGGTAATGGTGA+TGG 0.632946 5_4:-8658576 None:intergenic
GAAACTATTTGAGCTAAAGG+TGG 0.639226 5_4:-8658507 None:intergenic
AAACTAATAATAGAAACCAC+AGG 0.643581 5_4:-8659319 None:intergenic
TGGTGATGGTTGTTCTTGAG+GGG 0.643729 5_4:-8658562 None:intergenic
AAATAGGGAGAAGAAATTGG+TGG 0.650191 5_4:-8658537 None:intergenic
GCAATTTGTGGATAATGTGA+AGG 0.661579 5_4:-8658675 None:intergenic
TGTGGATAATGTGAAGGTGA+AGG 0.662098 5_4:-8658669 None:intergenic
TGCATGAAGAGTTGTTCCTG+TGG 0.663059 5_4:+8659303 Msa0835350:three_prime_UTR
GAACAATGAAGACCTTAACA+AGG 0.665682 5_4:+8657879 Msa0835350:CDS
CTGGATAAGGAGAACCAGCA+GGG 0.668605 5_4:-8658452 None:intergenic
GCAGGAGATACAAACTGTGG+TGG 0.675527 5_4:-8658588 None:intergenic
ACCAGCAGGGAAATCCAAAG+GGG 0.725497 5_4:-8658439 None:intergenic
TGGAGATTACTACTCCACAA+GGG 0.734669 5_4:+8658799 Msa0835350:CDS
CCAGCAGGGAAATCCAAAGG+GGG 0.739134 5_4:-8658438 None:intergenic
TTAGCAGGAGATACAAACTG+TGG 0.759496 5_4:-8658591 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTATAAACTTATATTATG+TGG + chr5_4:8659422-8659441 Msa0835350:three_prime_UTR 15.0%
!!! ATGTCTTAATTTTTGTTATT+AGG + chr5_4:8659463-8659482 Msa0835350:three_prime_UTR 15.0%
!!! TAATTTTTGTTATTAGGATT+TGG + chr5_4:8659469-8659488 Msa0835350:three_prime_UTR 15.0%
!! ATGTATAAACTTATATTATG+TGG + chr5_4:8659422-8659441 Msa0835350:three_prime_UTR 15.0%
!!! ATGTCTTAATTTTTGTTATT+AGG + chr5_4:8659463-8659482 Msa0835350:three_prime_UTR 15.0%
!!! TAATTTTTGTTATTAGGATT+TGG + chr5_4:8659469-8659488 Msa0835350:three_prime_UTR 15.0%
!! ACAAGAAAAGAAAATGAAAA+TGG + chr5_4:8657542-8657561 Msa0835350:exon 20.0%
!!! GTTTTAACATTTTCAAATCA+TGG - chr5_4:8658164-8658183 None:intergenic 20.0%
!! CAATGTTTATGATATTGTTA+TGG - chr5_4:8658323-8658342 None:intergenic 20.0%
!! TAAATTAATAAGCACTTACT+TGG - chr5_4:8658951-8658970 None:intergenic 20.0%
!! AAGTAAGTGCTTATTAATTT+AGG + chr5_4:8658950-8658969 Msa0835350:intron 20.0%
!! TGTTAATTAATAATGAGTGA+TGG + chr5_4:8659001-8659020 Msa0835350:intron 20.0%
!!! AATTAATTAGCTGTTTTAGA+TGG - chr5_4:8659173-8659192 None:intergenic 20.0%
!! TTACATTAATAATGGTGAAT+TGG + chr5_4:8659224-8659243 Msa0835350:intron 20.0%
!!! TCTAAATGAAAAGCATAATA+TGG - chr5_4:8659582-8659601 None:intergenic 20.0%
!! ACAAGAAAAGAAAATGAAAA+TGG + chr5_4:8657542-8657561 Msa0835350:exon 20.0%
!!! GTTTTAACATTTTCAAATCA+TGG - chr5_4:8658164-8658183 None:intergenic 20.0%
!! CAATGTTTATGATATTGTTA+TGG - chr5_4:8658323-8658342 None:intergenic 20.0%
!! TAAATTAATAAGCACTTACT+TGG - chr5_4:8658951-8658970 None:intergenic 20.0%
!! AAGTAAGTGCTTATTAATTT+AGG + chr5_4:8658950-8658969 Msa0835350:intron 20.0%
!! TGTTAATTAATAATGAGTGA+TGG + chr5_4:8659001-8659020 Msa0835350:intron 20.0%
!!! AATTAATTAGCTGTTTTAGA+TGG - chr5_4:8659173-8659192 None:intergenic 20.0%
!! TTACATTAATAATGGTGAAT+TGG + chr5_4:8659224-8659243 Msa0835350:intron 20.0%
!!! TCTAAATGAAAAGCATAATA+TGG - chr5_4:8659582-8659601 None:intergenic 20.0%
! AAAAGAAAATGAAAATGGTG+TGG + chr5_4:8657547-8657566 Msa0835350:exon 25.0%
!! TTTTTGATGAAGGGTAATAT+GGG - chr5_4:8657954-8657973 None:intergenic 25.0%
!! ATTTTTGATGAAGGGTAATA+TGG - chr5_4:8657955-8657974 None:intergenic 25.0%
! TTAGAATCCATGAAAAAACT+TGG + chr5_4:8658288-8658307 Msa0835350:CDS 25.0%
!!! GAAAAATCCAAGTTTTTTCA+TGG - chr5_4:8658298-8658317 None:intergenic 25.0%
!! TAGTGATTTTTCTCTCTTTT+GGG - chr5_4:8658356-8658375 None:intergenic 25.0%
! TTTGAATTTGGTACATTGTA+AGG - chr5_4:8658480-8658499 None:intergenic 25.0%
! TATAGTTATTGTACATAGCA+GGG - chr5_4:8659115-8659134 None:intergenic 25.0%
! TTATAGTTATTGTACATAGC+AGG - chr5_4:8659116-8659135 None:intergenic 25.0%
! ATAAAGTATGTAACTACCAA+AGG + chr5_4:8659132-8659151 Msa0835350:intron 25.0%
! AAACTAATAATAGAAACCAC+AGG - chr5_4:8659322-8659341 None:intergenic 25.0%
! AGACATAAATTCTCCAAATT+GGG - chr5_4:8659449-8659468 None:intergenic 25.0%
! AAGACATAAATTCTCCAAAT+TGG - chr5_4:8659450-8659469 None:intergenic 25.0%
!! TCACTAATCAGAATTTTGAA+GGG - chr5_4:8659528-8659547 None:intergenic 25.0%
!! ATCACTAATCAGAATTTTGA+AGG - chr5_4:8659529-8659548 None:intergenic 25.0%
! AAAAGAAAATGAAAATGGTG+TGG + chr5_4:8657547-8657566 Msa0835350:exon 25.0%
!! TTTTTGATGAAGGGTAATAT+GGG - chr5_4:8657954-8657973 None:intergenic 25.0%
!! ATTTTTGATGAAGGGTAATA+TGG - chr5_4:8657955-8657974 None:intergenic 25.0%
! TTAGAATCCATGAAAAAACT+TGG + chr5_4:8658288-8658307 Msa0835350:CDS 25.0%
!!! GAAAAATCCAAGTTTTTTCA+TGG - chr5_4:8658298-8658317 None:intergenic 25.0%
!! TAGTGATTTTTCTCTCTTTT+GGG - chr5_4:8658356-8658375 None:intergenic 25.0%
! TTTGAATTTGGTACATTGTA+AGG - chr5_4:8658480-8658499 None:intergenic 25.0%
! TATAGTTATTGTACATAGCA+GGG - chr5_4:8659115-8659134 None:intergenic 25.0%
! TTATAGTTATTGTACATAGC+AGG - chr5_4:8659116-8659135 None:intergenic 25.0%
! ATAAAGTATGTAACTACCAA+AGG + chr5_4:8659132-8659151 Msa0835350:intron 25.0%
! AAACTAATAATAGAAACCAC+AGG - chr5_4:8659322-8659341 None:intergenic 25.0%
! AGACATAAATTCTCCAAATT+GGG - chr5_4:8659449-8659468 None:intergenic 25.0%
! AAGACATAAATTCTCCAAAT+TGG - chr5_4:8659450-8659469 None:intergenic 25.0%
!! TCACTAATCAGAATTTTGAA+GGG - chr5_4:8659528-8659547 None:intergenic 25.0%
!! ATCACTAATCAGAATTTTGA+AGG - chr5_4:8659529-8659548 None:intergenic 25.0%
TTGTTCAAAGAAACAGCTAA+AGG - chr5_4:8657865-8657884 None:intergenic 30.0%
! ACATTTTATCACCTGAAACT+TGG + chr5_4:8657911-8657930 Msa0835350:CDS 30.0%
!!! TGTTGGTGATTTTTGATGAA+GGG - chr5_4:8657963-8657982 None:intergenic 30.0%
!!! ATGTTGGTGATTTTTGATGA+AGG - chr5_4:8657964-8657983 None:intergenic 30.0%
TCAAAAATCACCAACATTGT+TGG + chr5_4:8657967-8657986 Msa0835350:CDS 30.0%
! GAACAACATTAAGGTTGTTT+TGG - chr5_4:8658020-8658039 None:intergenic 30.0%
ACAGAAGTAGAACAACATTA+AGG - chr5_4:8658029-8658048 None:intergenic 30.0%
!! AGTTTCCATTGCTTTTGATT+TGG + chr5_4:8658107-8658126 Msa0835350:CDS 30.0%
AAATGTGAAAACCTTGAACT+TGG - chr5_4:8658234-8658253 None:intergenic 30.0%
!!! TTTTCTCTCTTTTGGGATAA+TGG - chr5_4:8658349-8658368 None:intergenic 30.0%
! GTAGTGATTTTTCTCTCTTT+TGG - chr5_4:8658357-8658376 None:intergenic 30.0%
GATGAAACTATTTGAGCTAA+AGG - chr5_4:8658513-8658532 None:intergenic 30.0%
TTGTTACAATCCTGATACAT+TGG + chr5_4:8658824-8658843 Msa0835350:CDS 30.0%
CTTATGCATTCAATAGTTAC+TGG + chr5_4:8658859-8658878 Msa0835350:CDS 30.0%
! TTAGATGGTTACAGTTTGTT+AGG - chr5_4:8659158-8659177 None:intergenic 30.0%
!! ATTGGTTATTGTTTTGTTGC+AGG + chr5_4:8659242-8659261 Msa0835350:intron 30.0%
ATCCTATGTTAAGATCACTT+TGG + chr5_4:8659361-8659380 Msa0835350:three_prime_UTR 30.0%
AACCAAAGTGATCTTAACAT+AGG - chr5_4:8659366-8659385 None:intergenic 30.0%
TATATTATGTGGACCCAATT+TGG + chr5_4:8659433-8659452 Msa0835350:three_prime_UTR 30.0%
TTGTTCAAAGAAACAGCTAA+AGG - chr5_4:8657865-8657884 None:intergenic 30.0%
! ACATTTTATCACCTGAAACT+TGG + chr5_4:8657911-8657930 Msa0835350:CDS 30.0%
!!! TGTTGGTGATTTTTGATGAA+GGG - chr5_4:8657963-8657982 None:intergenic 30.0%
!!! ATGTTGGTGATTTTTGATGA+AGG - chr5_4:8657964-8657983 None:intergenic 30.0%
TCAAAAATCACCAACATTGT+TGG + chr5_4:8657967-8657986 Msa0835350:CDS 30.0%
! GAACAACATTAAGGTTGTTT+TGG - chr5_4:8658020-8658039 None:intergenic 30.0%
ACAGAAGTAGAACAACATTA+AGG - chr5_4:8658029-8658048 None:intergenic 30.0%
!! AGTTTCCATTGCTTTTGATT+TGG + chr5_4:8658107-8658126 Msa0835350:CDS 30.0%
AAATGTGAAAACCTTGAACT+TGG - chr5_4:8658234-8658253 None:intergenic 30.0%
!!! TTTTCTCTCTTTTGGGATAA+TGG - chr5_4:8658349-8658368 None:intergenic 30.0%
! GTAGTGATTTTTCTCTCTTT+TGG - chr5_4:8658357-8658376 None:intergenic 30.0%
GATGAAACTATTTGAGCTAA+AGG - chr5_4:8658513-8658532 None:intergenic 30.0%
TTGTTACAATCCTGATACAT+TGG + chr5_4:8658824-8658843 Msa0835350:CDS 30.0%
CTTATGCATTCAATAGTTAC+TGG + chr5_4:8658859-8658878 Msa0835350:CDS 30.0%
! TTAGATGGTTACAGTTTGTT+AGG - chr5_4:8659158-8659177 None:intergenic 30.0%
!! ATTGGTTATTGTTTTGTTGC+AGG + chr5_4:8659242-8659261 Msa0835350:intron 30.0%
ATCCTATGTTAAGATCACTT+TGG + chr5_4:8659361-8659380 Msa0835350:three_prime_UTR 30.0%
AACCAAAGTGATCTTAACAT+AGG - chr5_4:8659366-8659385 None:intergenic 30.0%
TATATTATGTGGACCCAATT+TGG + chr5_4:8659433-8659452 Msa0835350:three_prime_UTR 30.0%
GAGAAGAACCTTGAGATTAT+TGG + chr5_4:8657518-8657537 Msa0835350:five_prime_UTR 35.0%
TTCTTGTGCCAATAATCTCA+AGG - chr5_4:8657529-8657548 None:intergenic 35.0%
CACTTGTTTCACATAGGTTT+AGG - chr5_4:8657807-8657826 None:intergenic 35.0%
TGTCTTCACTTGTTTCACAT+AGG - chr5_4:8657813-8657832 None:intergenic 35.0%
GAACAATGAAGACCTTAACA+AGG + chr5_4:8657879-8657898 Msa0835350:CDS 35.0%
TTTAACCTAAGCCAAGTTTC+AGG - chr5_4:8657925-8657944 None:intergenic 35.0%
! TGAGGTTTTTCCAACAATGT+TGG - chr5_4:8657980-8657999 None:intergenic 35.0%
!!! TAAGGTTGTTTTGGTCTTGT+TGG - chr5_4:8658011-8658030 None:intergenic 35.0%
TCTATCATTCCCTCAAAAGA+TGG + chr5_4:8658064-8658083 Msa0835350:CDS 35.0%
CTATCATTCCCTCAAAAGAT+GGG + chr5_4:8658065-8658084 Msa0835350:CDS 35.0%
TATCATTCCCTCAAAAGATG+GGG + chr5_4:8658066-8658085 Msa0835350:CDS 35.0%
! TTCTAAACCCCATCTTTTGA+GGG - chr5_4:8658076-8658095 None:intergenic 35.0%
ACAGTCCAAATCAAAAGCAA+TGG - chr5_4:8658115-8658134 None:intergenic 35.0%
! GTTTGAAGTAGCTGAGTTAA+GGG - chr5_4:8658142-8658161 None:intergenic 35.0%
GCTTGAAGAAACTCAATCAT+AGG - chr5_4:8658186-8658205 None:intergenic 35.0%
TTTCTCTCATACCAAGTTCA+AGG + chr5_4:8658220-8658239 Msa0835350:CDS 35.0%
!!! GGTTGGTTTTATAGGAATTG+TGG - chr5_4:8658397-8658416 None:intergenic 35.0%
!! TTGAGCAATTTCTGGAATTG+GGG - chr5_4:8658418-8658437 None:intergenic 35.0%
!! GTTGAGCAATTTCTGGAATT+GGG - chr5_4:8658419-8658438 None:intergenic 35.0%
!!! GGTGGTAAAGGTTTTGAATT+TGG - chr5_4:8658492-8658511 None:intergenic 35.0%
ATTTGAGCTAAAGGTGGTAA+AGG - chr5_4:8658504-8658523 None:intergenic 35.0%
GAAACTATTTGAGCTAAAGG+TGG - chr5_4:8658510-8658529 None:intergenic 35.0%
AAATAGGGAGAAGAAATTGG+TGG - chr5_4:8658540-8658559 None:intergenic 35.0%
GCAAAATAGGGAGAAGAAAT+TGG - chr5_4:8658543-8658562 None:intergenic 35.0%
! TTTTCTATTGCATTGCAAGG+TGG - chr5_4:8658642-8658661 None:intergenic 35.0%
! CCATTTTCTATTGCATTGCA+AGG - chr5_4:8658645-8658664 None:intergenic 35.0%
CCTTGCAATGCAATAGAAAA+TGG + chr5_4:8658642-8658661 Msa0835350:CDS 35.0%
GCAATTTGTGGATAATGTGA+AGG - chr5_4:8658678-8658697 None:intergenic 35.0%
! TGGCAAAAGCATAAGAGAAT+TGG + chr5_4:8658879-8658898 Msa0835350:CDS 35.0%
! CAAAAGCATAAGAGAATTGG+TGG + chr5_4:8658882-8658901 Msa0835350:CDS 35.0%
TGAGAATAAACCTACAGTGA+AGG - chr5_4:8659271-8659290 None:intergenic 35.0%
AGATCACTTTGGTTGTTGAT+TGG + chr5_4:8659372-8659391 Msa0835350:three_prime_UTR 35.0%
GAGAAGAACCTTGAGATTAT+TGG + chr5_4:8657518-8657537 Msa0835350:five_prime_UTR 35.0%
TTCTTGTGCCAATAATCTCA+AGG - chr5_4:8657529-8657548 None:intergenic 35.0%
CACTTGTTTCACATAGGTTT+AGG - chr5_4:8657807-8657826 None:intergenic 35.0%
TGTCTTCACTTGTTTCACAT+AGG - chr5_4:8657813-8657832 None:intergenic 35.0%
GAACAATGAAGACCTTAACA+AGG + chr5_4:8657879-8657898 Msa0835350:CDS 35.0%
TTTAACCTAAGCCAAGTTTC+AGG - chr5_4:8657925-8657944 None:intergenic 35.0%
! TGAGGTTTTTCCAACAATGT+TGG - chr5_4:8657980-8657999 None:intergenic 35.0%
!!! TAAGGTTGTTTTGGTCTTGT+TGG - chr5_4:8658011-8658030 None:intergenic 35.0%
TCTATCATTCCCTCAAAAGA+TGG + chr5_4:8658064-8658083 Msa0835350:CDS 35.0%
CTATCATTCCCTCAAAAGAT+GGG + chr5_4:8658065-8658084 Msa0835350:CDS 35.0%
TATCATTCCCTCAAAAGATG+GGG + chr5_4:8658066-8658085 Msa0835350:CDS 35.0%
! TTCTAAACCCCATCTTTTGA+GGG - chr5_4:8658076-8658095 None:intergenic 35.0%
ACAGTCCAAATCAAAAGCAA+TGG - chr5_4:8658115-8658134 None:intergenic 35.0%
! GTTTGAAGTAGCTGAGTTAA+GGG - chr5_4:8658142-8658161 None:intergenic 35.0%
GCTTGAAGAAACTCAATCAT+AGG - chr5_4:8658186-8658205 None:intergenic 35.0%
TTTCTCTCATACCAAGTTCA+AGG + chr5_4:8658220-8658239 Msa0835350:CDS 35.0%
!!! GGTTGGTTTTATAGGAATTG+TGG - chr5_4:8658397-8658416 None:intergenic 35.0%
!! TTGAGCAATTTCTGGAATTG+GGG - chr5_4:8658418-8658437 None:intergenic 35.0%
!! GTTGAGCAATTTCTGGAATT+GGG - chr5_4:8658419-8658438 None:intergenic 35.0%
!!! GGTGGTAAAGGTTTTGAATT+TGG - chr5_4:8658492-8658511 None:intergenic 35.0%
ATTTGAGCTAAAGGTGGTAA+AGG - chr5_4:8658504-8658523 None:intergenic 35.0%
GAAACTATTTGAGCTAAAGG+TGG - chr5_4:8658510-8658529 None:intergenic 35.0%
AAATAGGGAGAAGAAATTGG+TGG - chr5_4:8658540-8658559 None:intergenic 35.0%
GCAAAATAGGGAGAAGAAAT+TGG - chr5_4:8658543-8658562 None:intergenic 35.0%
! TTTTCTATTGCATTGCAAGG+TGG - chr5_4:8658642-8658661 None:intergenic 35.0%
! CCATTTTCTATTGCATTGCA+AGG - chr5_4:8658645-8658664 None:intergenic 35.0%
CCTTGCAATGCAATAGAAAA+TGG + chr5_4:8658642-8658661 Msa0835350:CDS 35.0%
GCAATTTGTGGATAATGTGA+AGG - chr5_4:8658678-8658697 None:intergenic 35.0%
! TGGCAAAAGCATAAGAGAAT+TGG + chr5_4:8658879-8658898 Msa0835350:CDS 35.0%
! CAAAAGCATAAGAGAATTGG+TGG + chr5_4:8658882-8658901 Msa0835350:CDS 35.0%
TGAGAATAAACCTACAGTGA+AGG - chr5_4:8659271-8659290 None:intergenic 35.0%
AGATCACTTTGGTTGTTGAT+TGG + chr5_4:8659372-8659391 Msa0835350:three_prime_UTR 35.0%
CATCTTCTCTTTCTTTGCTC+TGG + chr5_4:8657576-8657595 Msa0835350:CDS 40.0%
! GTAGTTTGCATTGCAGTTTC+TGG + chr5_4:8657763-8657782 Msa0835350:intron 40.0%
TGTTGCTAGAGACCTTGTTA+AGG - chr5_4:8657894-8657913 None:intergenic 40.0%
TATCACCTGAAACTTGGCTT+AGG + chr5_4:8657917-8657936 Msa0835350:CDS 40.0%
!! GTTCTAAACCCCATCTTTTG+AGG - chr5_4:8658077-8658096 None:intergenic 40.0%
! GGTTTGAAGTAGCTGAGTTA+AGG - chr5_4:8658143-8658162 None:intergenic 40.0%
!!! GGAATTGGGGTTGGTTTTAT+AGG - chr5_4:8658405-8658424 None:intergenic 40.0%
!! GGTTGAGCAATTTCTGGAAT+TGG - chr5_4:8658420-8658439 None:intergenic 40.0%
ACATTGTAAGGAGCTGGATA+AGG - chr5_4:8658468-8658487 None:intergenic 40.0%
TTTGGTACATTGTAAGGAGC+TGG - chr5_4:8658474-8658493 None:intergenic 40.0%
TGTTCTTGAGGGGCAAAATA+GGG - chr5_4:8658555-8658574 None:intergenic 40.0%
TTGTTCTTGAGGGGCAAAAT+AGG - chr5_4:8658556-8658575 None:intergenic 40.0%
!! ATGGTGATGGTTGTTCTTGA+GGG - chr5_4:8658566-8658585 None:intergenic 40.0%
!! AATGGTGATGGTTGTTCTTG+AGG - chr5_4:8658567-8658586 None:intergenic 40.0%
GATACAAACTGTGGTGGTAA+TGG - chr5_4:8658585-8658604 None:intergenic 40.0%
TTAGCAGGAGATACAAACTG+TGG - chr5_4:8658594-8658613 None:intergenic 40.0%
! GGACAAGTGAAACCATTTTG+AGG - chr5_4:8658621-8658640 None:intergenic 40.0%
TGTGGATAATGTGAAGGTGA+AGG - chr5_4:8658672-8658691 None:intergenic 40.0%
TTCTGAACAGGTGCAATTTG+TGG - chr5_4:8658690-8658709 None:intergenic 40.0%
TTGCACCTGTTCAGAAACTT+TGG + chr5_4:8658694-8658713 Msa0835350:CDS 40.0%
ACACACCAAAGTTTCTGAAC+AGG - chr5_4:8658702-8658721 None:intergenic 40.0%
AACGTTTCATCAGGAACACT+AGG - chr5_4:8658732-8658751 None:intergenic 40.0%
TGAAACGTTGCAAGAAGCTT+TGG + chr5_4:8658743-8658762 Msa0835350:CDS 40.0%
!! GAAGCTTTGGATTATGCTTG+TGG + chr5_4:8658756-8658775 Msa0835350:CDS 40.0%
TTGGATTATGCTTGTGGTGA+AGG + chr5_4:8658762-8658781 Msa0835350:CDS 40.0%
TTGGAGATTACTACTCCACA+AGG + chr5_4:8658798-8658817 Msa0835350:CDS 40.0%
TGGAGATTACTACTCCACAA+GGG + chr5_4:8658799-8658818 Msa0835350:CDS 40.0%
GGATTGTAACAATTCCCTTG+TGG - chr5_4:8658816-8658835 None:intergenic 40.0%
ATTGGTGGAACTTGTGACTT+TGG + chr5_4:8658897-8658916 Msa0835350:CDS 40.0%
AGTGATGGTTGTGTTGTGAA+TGG + chr5_4:8659016-8659035 Msa0835350:intron 40.0%
!! GTTACAGTTTGTTAGGCCTT+TGG - chr5_4:8659151-8659170 None:intergenic 40.0%
TTTGCTACTAGCTAGCTAGT+TGG + chr5_4:8659396-8659415 Msa0835350:three_prime_UTR 40.0%
CATCTTCTCTTTCTTTGCTC+TGG + chr5_4:8657576-8657595 Msa0835350:CDS 40.0%
! GTAGTTTGCATTGCAGTTTC+TGG + chr5_4:8657763-8657782 Msa0835350:intron 40.0%
TGTTGCTAGAGACCTTGTTA+AGG - chr5_4:8657894-8657913 None:intergenic 40.0%
TATCACCTGAAACTTGGCTT+AGG + chr5_4:8657917-8657936 Msa0835350:CDS 40.0%
!! GTTCTAAACCCCATCTTTTG+AGG - chr5_4:8658077-8658096 None:intergenic 40.0%
! GGTTTGAAGTAGCTGAGTTA+AGG - chr5_4:8658143-8658162 None:intergenic 40.0%
!!! GGAATTGGGGTTGGTTTTAT+AGG - chr5_4:8658405-8658424 None:intergenic 40.0%
!! GGTTGAGCAATTTCTGGAAT+TGG - chr5_4:8658420-8658439 None:intergenic 40.0%
ACATTGTAAGGAGCTGGATA+AGG - chr5_4:8658468-8658487 None:intergenic 40.0%
TTTGGTACATTGTAAGGAGC+TGG - chr5_4:8658474-8658493 None:intergenic 40.0%
TGTTCTTGAGGGGCAAAATA+GGG - chr5_4:8658555-8658574 None:intergenic 40.0%
TTGTTCTTGAGGGGCAAAAT+AGG - chr5_4:8658556-8658575 None:intergenic 40.0%
!! ATGGTGATGGTTGTTCTTGA+GGG - chr5_4:8658566-8658585 None:intergenic 40.0%
!! AATGGTGATGGTTGTTCTTG+AGG - chr5_4:8658567-8658586 None:intergenic 40.0%
GATACAAACTGTGGTGGTAA+TGG - chr5_4:8658585-8658604 None:intergenic 40.0%
TTAGCAGGAGATACAAACTG+TGG - chr5_4:8658594-8658613 None:intergenic 40.0%
! GGACAAGTGAAACCATTTTG+AGG - chr5_4:8658621-8658640 None:intergenic 40.0%
TGTGGATAATGTGAAGGTGA+AGG - chr5_4:8658672-8658691 None:intergenic 40.0%
TTCTGAACAGGTGCAATTTG+TGG - chr5_4:8658690-8658709 None:intergenic 40.0%
TTGCACCTGTTCAGAAACTT+TGG + chr5_4:8658694-8658713 Msa0835350:CDS 40.0%
ACACACCAAAGTTTCTGAAC+AGG - chr5_4:8658702-8658721 None:intergenic 40.0%
AACGTTTCATCAGGAACACT+AGG - chr5_4:8658732-8658751 None:intergenic 40.0%
TGAAACGTTGCAAGAAGCTT+TGG + chr5_4:8658743-8658762 Msa0835350:CDS 40.0%
!! GAAGCTTTGGATTATGCTTG+TGG + chr5_4:8658756-8658775 Msa0835350:CDS 40.0%
TTGGATTATGCTTGTGGTGA+AGG + chr5_4:8658762-8658781 Msa0835350:CDS 40.0%
TTGGAGATTACTACTCCACA+AGG + chr5_4:8658798-8658817 Msa0835350:CDS 40.0%
TGGAGATTACTACTCCACAA+GGG + chr5_4:8658799-8658818 Msa0835350:CDS 40.0%
GGATTGTAACAATTCCCTTG+TGG - chr5_4:8658816-8658835 None:intergenic 40.0%
ATTGGTGGAACTTGTGACTT+TGG + chr5_4:8658897-8658916 Msa0835350:CDS 40.0%
AGTGATGGTTGTGTTGTGAA+TGG + chr5_4:8659016-8659035 Msa0835350:intron 40.0%
!! GTTACAGTTTGTTAGGCCTT+TGG - chr5_4:8659151-8659170 None:intergenic 40.0%
TTTGCTACTAGCTAGCTAGT+TGG + chr5_4:8659396-8659415 Msa0835350:three_prime_UTR 40.0%
! GTCTTGTTGGCAGAAAGTTG+AGG - chr5_4:8657998-8658017 None:intergenic 45.0%
!! GCAATTTCTGGAATTGGGGT+TGG - chr5_4:8658414-8658433 None:intergenic 45.0%
AAAGGGGGTTGAGCAATTTC+TGG - chr5_4:8658426-8658445 None:intergenic 45.0%
AGAAATTGCTCAACCCCCTT+TGG + chr5_4:8658425-8658444 Msa0835350:CDS 45.0%
AACCAGCAGGGAAATCCAAA+GGG - chr5_4:8658443-8658462 None:intergenic 45.0%
TAGGGAGAAGAAATTGGTGG+TGG - chr5_4:8658537-8658556 None:intergenic 45.0%
!! TGGTGATGGTTGTTCTTGAG+GGG - chr5_4:8658565-8658584 None:intergenic 45.0%
!! AACTGTGGTGGTAATGGTGA+TGG - chr5_4:8658579-8658598 None:intergenic 45.0%
!! CCATTTTGAGGAGAGTTAGC+AGG - chr5_4:8658609-8658628 None:intergenic 45.0%
CCTGCTAACTCTCCTCAAAA+TGG + chr5_4:8658606-8658625 Msa0835350:CDS 45.0%
GCTTCTTGCAACGTTTCATC+AGG - chr5_4:8658741-8658760 None:intergenic 45.0%
GATTATGCTTGTGGTGAAGG+TGG + chr5_4:8658765-8658784 Msa0835350:CDS 45.0%
!! TGAAGGTGGTGCTGATTGTT+TGG + chr5_4:8658779-8658798 Msa0835350:CDS 45.0%
GCATGAGCAACCAATGTATC+AGG - chr5_4:8658837-8658856 None:intergenic 45.0%
GTGGAACTTGTGACTTTGGT+GGG + chr5_4:8658901-8658920 Msa0835350:CDS 45.0%
TTGCAGGTTTCCTTCACTGT+AGG + chr5_4:8659258-8659277 Msa0835350:intron 45.0%
TGCATGAAGAGTTGTTCCTG+TGG + chr5_4:8659303-8659322 Msa0835350:three_prime_UTR 45.0%
! GTCTTGTTGGCAGAAAGTTG+AGG - chr5_4:8657998-8658017 None:intergenic 45.0%
!! GCAATTTCTGGAATTGGGGT+TGG - chr5_4:8658414-8658433 None:intergenic 45.0%
AAAGGGGGTTGAGCAATTTC+TGG - chr5_4:8658426-8658445 None:intergenic 45.0%
AGAAATTGCTCAACCCCCTT+TGG + chr5_4:8658425-8658444 Msa0835350:CDS 45.0%
AACCAGCAGGGAAATCCAAA+GGG - chr5_4:8658443-8658462 None:intergenic 45.0%
TAGGGAGAAGAAATTGGTGG+TGG - chr5_4:8658537-8658556 None:intergenic 45.0%
!! TGGTGATGGTTGTTCTTGAG+GGG - chr5_4:8658565-8658584 None:intergenic 45.0%
!! AACTGTGGTGGTAATGGTGA+TGG - chr5_4:8658579-8658598 None:intergenic 45.0%
!! CCATTTTGAGGAGAGTTAGC+AGG - chr5_4:8658609-8658628 None:intergenic 45.0%
CCTGCTAACTCTCCTCAAAA+TGG + chr5_4:8658606-8658625 Msa0835350:CDS 45.0%
GCTTCTTGCAACGTTTCATC+AGG - chr5_4:8658741-8658760 None:intergenic 45.0%
GATTATGCTTGTGGTGAAGG+TGG + chr5_4:8658765-8658784 Msa0835350:CDS 45.0%
!! TGAAGGTGGTGCTGATTGTT+TGG + chr5_4:8658779-8658798 Msa0835350:CDS 45.0%
GCATGAGCAACCAATGTATC+AGG - chr5_4:8658837-8658856 None:intergenic 45.0%
GTGGAACTTGTGACTTTGGT+GGG + chr5_4:8658901-8658920 Msa0835350:CDS 45.0%
TTGCAGGTTTCCTTCACTGT+AGG + chr5_4:8659258-8659277 Msa0835350:intron 45.0%
TGCATGAAGAGTTGTTCCTG+TGG + chr5_4:8659303-8659322 Msa0835350:three_prime_UTR 45.0%
!! TTATTTATTTACATTAATAA+TGG + chr5_4:8659216-8659235 Msa0835350:intron 5.0%
!! TTATTTATTTACATTAATAA+TGG + chr5_4:8659216-8659235 Msa0835350:intron 5.0%
ACCAGCAGGGAAATCCAAAG+GGG - chr5_4:8658442-8658461 None:intergenic 50.0%
GAACCAGCAGGGAAATCCAA+AGG - chr5_4:8658444-8658463 None:intergenic 50.0%
CTGGATAAGGAGAACCAGCA+GGG - chr5_4:8658455-8658474 None:intergenic 50.0%
GCAGGAGATACAAACTGTGG+TGG - chr5_4:8658591-8658610 None:intergenic 50.0%
GGTGGAACTTGTGACTTTGG+TGG + chr5_4:8658900-8658919 Msa0835350:CDS 50.0%
ACCAGCAGGGAAATCCAAAG+GGG - chr5_4:8658442-8658461 None:intergenic 50.0%
GAACCAGCAGGGAAATCCAA+AGG - chr5_4:8658444-8658463 None:intergenic 50.0%
CTGGATAAGGAGAACCAGCA+GGG - chr5_4:8658455-8658474 None:intergenic 50.0%
GCAGGAGATACAAACTGTGG+TGG - chr5_4:8658591-8658610 None:intergenic 50.0%
GGTGGAACTTGTGACTTTGG+TGG + chr5_4:8658900-8658919 Msa0835350:CDS 50.0%
CCAGCAGGGAAATCCAAAGG+GGG - chr5_4:8658441-8658460 None:intergenic 55.0%
GCTGGATAAGGAGAACCAGC+AGG - chr5_4:8658456-8658475 None:intergenic 55.0%
CCAGCAGGGAAATCCAAAGG+GGG - chr5_4:8658441-8658460 None:intergenic 55.0%
GCTGGATAAGGAGAACCAGC+AGG - chr5_4:8658456-8658475 None:intergenic 55.0%
CCCCCTTTGGATTTCCCTGC+TGG + chr5_4:8658438-8658457 Msa0835350:CDS 60.0%
CCCCCTTTGGATTTCCCTGC+TGG + chr5_4:8658438-8658457 Msa0835350:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 8657491 8659607 8657491 ID=Msa0835350;Name=Msa0835350
chr5_4 mRNA 8657491 8659607 8657491 ID=Msa0835350-mRNA-1;Parent=Msa0835350;Name=Msa0835350-mRNA-1;_AED=0.01;_eAED=0.01;_QI=64|1|1|1|1|1|3|312|419
chr5_4 exon 8657491 8657597 8657491 ID=Msa0835350-mRNA-1:exon:3801;Parent=Msa0835350-mRNA-1
chr5_4 exon 8657767 8658951 8657767 ID=Msa0835350-mRNA-1:exon:3802;Parent=Msa0835350-mRNA-1
chr5_4 exon 8659264 8659607 8659264 ID=Msa0835350-mRNA-1:exon:3803;Parent=Msa0835350-mRNA-1
chr5_4 five_prime_UTR 8657491 8657554 8657491 ID=Msa0835350-mRNA-1:five_prime_utr;Parent=Msa0835350-mRNA-1
chr5_4 CDS 8657555 8657597 8657555 ID=Msa0835350-mRNA-1:cds;Parent=Msa0835350-mRNA-1
chr5_4 CDS 8657767 8658951 8657767 ID=Msa0835350-mRNA-1:cds;Parent=Msa0835350-mRNA-1
chr5_4 CDS 8659264 8659295 8659264 ID=Msa0835350-mRNA-1:cds;Parent=Msa0835350-mRNA-1
chr5_4 three_prime_UTR 8659296 8659607 8659296 ID=Msa0835350-mRNA-1:three_prime_utr;Parent=Msa0835350-mRNA-1
Gene Sequence

>Msa0835350

ATGAAAATGGTGTGGCTATTTCATCTTCTCTTTCTTTGCTCTGTTTGCATTGCAGTTTCTGGTCAACAAAACATTGAGTTCCTAAACCTATGTGAAACAAGTGAAGACATTGCAACATCTTCATCTCACAGTGAGTTTCCTTTAGCTGTTTCTTTGAACAATGAAGACCTTAACAAGGTCTCTAGCAACATTTTATCACCTGAAACTTGGCTTAGGTTAAACATTTTCCCATATTACCCTTCATCAAAAATCACCAACATTGTTGGAAAAACCTCAACTTTCTGCCAACAAGACCAAAACAACCTTAATGTTGTTCTACTTCTGTCTTCTTTGAAAAATCTCTATCATTCCCTCAAAAGATGGGGTTTAGAACAAGACATCAAAGTTTCCATTGCTTTTGATTTGGACTGTTTTACCCTTAACTCAGCTACTTCAAACCATGATTTGAAAATGTTAAAACCTATGATTGAGTTTCTTCAAGCTGTTAACTCAACATTTTCTCTCATACCAAGTTCAAGGTTTTCACATTTTTCTGATAAAAGTTTAAACTTTGTGTCTTCTCAATTAGAATCCATGAAAAAACTTGGATTTTTCAACCATAACAATATCATAAACATTGCTACCATTATCCCAAAAGAGAGAAAAATCACTACTAGAAAACTTTCAGTTACCACAATTCCTATAAAACCAACCCCAATTCCAGAAATTGCTCAACCCCCTTTGGATTTCCCTGCTGGTTCTCCTTATCCAGCTCCTTACAATGTACCAAATTCAAAACCTTTACCACCTTTAGCTCAAATAGTTTCATCTCCACCACCAATTTCTTCTCCCTATTTTGCCCCTCAAGAACAACCATCACCATTACCACCACAGTTTGTATCTCCTGCTAACTCTCCTCAAAATGGTTTCACTTGTCCACCTTGCAATGCAATAGAAAATGGTTCACCTTCACCTTCACATTATCCACAAATTGCACCTGTTCAGAAACTTTGGTGTGTTGCAAAGCCTAGTGTTCCTGATGAAACGTTGCAAGAAGCTTTGGATTATGCTTGTGGTGAAGGTGGTGCTGATTGTTTGGAGATTACTACTCCACAAGGGAATTGTTACAATCCTGATACATTGGTTGCTCATGCTTCTTATGCATTCAATAGTTACTGGCAAAAGCATAAGAGAATTGGTGGAACTTGTGACTTTGGTGGGACTGCTATGTTGATTCATTCTGATCCAAGTTTCCTTCACTGTAGGTTTATTCTCAGCTAG
Protein sequence

>Msa0835350

MKMVWLFHLLFLCSVCIAVSGQQNIEFLNLCETSEDIATSSSHSEFPLAVSLNNEDLNKVSSNILSPETWLRLNIFPYYPSSKITNIVGKTSTFCQQDQNNLNVVLLLSSLKNLYHSLKRWGLEQDIKVSIAFDLDCFTLNSATSNHDLKMLKPMIEFLQAVNSTFSLIPSSRFSHFSDKSLNFVSSQLESMKKLGFFNHNNIINIATIIPKERKITTRKLSVTTIPIKPTPIPEIAQPPLDFPAGSPYPAPYNVPNSKPLPPLAQIVSSPPPISSPYFAPQEQPSPLPPQFVSPANSPQNGFTCPPCNAIENGSPSPSHYPQIAPVQKLWCVAKPSVPDETLQEALDYACGEGGADCLEITTPQGNCYNPDTLVAHASYAFNSYWQKHKRIGGTCDFGGTAMLIHSDPSFLHCRFILS*