AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1400010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1400010 MtrunA17_Chr5g0401301 96.181 419 14 1 1 419 1 417 0.0 815
Msa1400010 MtrunA17_Chr1g0177171 27.880 434 268 12 23 416 62 490 1.87e-38 145
Msa1400010 MtrunA17_Chr3g0120401 61.538 91 33 2 326 416 152 240 3.82e-32 122
Msa1400010 MtrunA17_Chr1g0213651 60.215 93 35 2 325 417 270 360 4.41e-31 122
Msa1400010 MtrunA17_Chr7g0234821 24.018 433 264 11 24 416 51 458 2.71e-28 115
Msa1400010 MtrunA17_Chr8g0339191 25.935 428 251 15 28 416 58 458 3.96e-28 115
Msa1400010 MtrunA17_Chr5g0439251 53.191 94 37 2 331 418 382 474 1.26e-25 108
Msa1400010 MtrunA17_Chr5g0447651 54.545 88 39 1 329 416 28 114 1.09e-24 98.2
Msa1400010 MtrunA17_Chr8g0378361 52.326 86 39 2 331 416 30 113 1.23e-23 97.4
Msa1400010 MtrunA17_Chr3g0096451 50.000 86 41 2 331 416 112 195 5.08e-23 97.4
Msa1400010 MtrunA17_Chr8g0378621 49.425 87 43 1 330 416 363 448 6.21e-23 100
Msa1400010 MtrunA17_Chr7g0271411 53.488 86 38 2 331 416 160 243 8.24e-23 97.8
Msa1400010 MtrunA17_Chr8g0378371 49.462 93 42 3 324 416 26 113 1.73e-22 92.0
Msa1400010 MtrunA17_Chr4g0076141 51.220 82 39 1 331 412 13 93 1.42e-21 89.0
Msa1400010 MtrunA17_Chr8g0381081 51.163 86 41 1 331 416 57 141 1.52e-21 91.3
Msa1400010 MtrunA17_Chr1g0193131 60.494 81 27 4 331 410 420 496 5.48e-21 95.5
Msa1400010 MtrunA17_Chr5g0443331 50.000 86 41 2 331 416 108 191 1.05e-20 91.3
Msa1400010 MtrunA17_Chr4g0075031 47.727 88 45 1 329 416 380 466 1.72e-20 93.6
Msa1400010 MtrunA17_Chr4g0076131 45.455 88 43 2 331 416 28 112 3.22e-20 85.9
Msa1400010 MtrunA17_Chr8g0378381 45.455 88 46 2 329 416 30 115 9.64e-20 84.3
Msa1400010 MtrunA17_Chr3g0121271 45.455 88 47 1 329 416 360 446 5.68e-19 89.0
Msa1400010 MtrunA17_Chr4g0040911 46.067 89 47 1 328 416 34 121 1.21e-18 83.2
Msa1400010 MtrunA17_Chr2g0304061 51.685 89 40 3 331 419 158 243 1.26e-18 86.3
Msa1400010 MtrunA17_Chr8g0389741 43.678 87 48 1 331 417 368 453 2.32e-18 87.0
Msa1400010 MtrunA17_Chr4g0061401 49.462 93 40 4 326 416 19 106 3.37e-18 82.8
Msa1400010 MtrunA17_Chr8g0378401 47.561 82 41 2 329 410 30 109 6.22e-18 79.3
Msa1400010 MtrunA17_Chr1g0161011 44.944 89 48 1 328 416 358 445 6.59e-18 85.5
Msa1400010 MtrunA17_Chr4g0034901 40.000 110 62 2 310 416 351 459 8.42e-18 85.1
Msa1400010 MtrunA17_Chr6g0487261 43.182 88 48 2 331 417 369 455 1.30e-17 84.7
Msa1400010 MtrunA17_Chr4g0046311 23.333 420 265 13 28 410 52 451 2.18e-17 84.0
Msa1400010 MtrunA17_Chr4g0043511 44.318 88 47 2 329 416 418 503 4.44e-17 83.2
Msa1400010 MtrunA17_Chr5g0434591 41.573 89 51 1 328 416 357 444 1.23e-16 81.6
Msa1400010 MtrunA17_Chr8g0380231 55.882 68 28 2 343 410 29 94 1.48e-16 75.1
Msa1400010 MtrunA17_Chr5g0419121 50.685 73 34 2 330 402 21 91 3.35e-16 78.2
Msa1400010 MtrunA17_Chr1g0154991 43.023 86 47 2 331 416 20 103 1.51e-15 72.4
Msa1400010 MtrunA17_Chr4g0043151 44.444 81 43 2 330 410 20 98 2.01e-15 74.3
Msa1400010 MtrunA17_Chr3g0110371 42.529 87 47 2 331 416 361 445 7.36e-15 76.3
Msa1400010 MtrunA17_Chr3g0144531 41.860 86 48 2 331 416 371 454 2.40e-14 74.7
Msa1400010 MtrunA17_Chr2g0313891 39.080 87 50 1 328 414 27 110 6.68e-13 65.1
Msa1400010 MtrunA17_Chr7g0223961 35.000 100 63 2 316 415 350 447 2.78e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1400010 AT5G67460.1 42.227 431 177 17 6 419 5 380 1.18e-82 258
Msa1400010 AT5G67460.2 41.647 425 176 17 6 413 5 374 1.83e-77 245
Msa1400010 AT5G55180.1 28.873 426 251 14 23 416 51 456 1.68e-39 147
Msa1400010 AT5G55180.2 29.048 420 246 14 23 410 51 450 1.51e-37 142
Msa1400010 AT1G79480.2 58.065 93 37 2 324 416 303 393 8.39e-31 122
Msa1400010 AT1G79480.1 58.065 93 37 2 324 416 304 394 8.43e-31 122
Msa1400010 AT2G05790.1 58.140 86 35 1 331 416 384 468 2.63e-27 113
Msa1400010 AT4G26830.2 58.140 86 35 1 331 416 371 455 2.36e-26 110
Msa1400010 AT4G26830.1 58.140 86 35 1 331 416 367 451 2.50e-26 110
Msa1400010 AT2G30933.1 57.143 91 37 2 326 416 77 165 4.12e-26 105
Msa1400010 AT2G30933.2 58.824 85 33 2 326 410 77 159 8.22e-25 100
Msa1400010 AT2G30933.3 58.824 85 33 2 326 410 77 159 8.22e-25 100
Msa1400010 AT5G35740.1 48.837 86 44 0 331 416 30 115 1.90e-24 97.8
Msa1400010 AT3G07320.1 24.942 429 263 16 24 416 50 455 1.36e-23 102
Msa1400010 AT1G66855.1 45.455 88 47 1 329 416 24 110 1.22e-22 92.4
Msa1400010 AT2G04910.2 47.674 86 44 1 331 416 15 99 6.19e-22 90.5
Msa1400010 AT4G05430.1 46.512 86 45 1 331 416 23 107 3.08e-21 89.7
Msa1400010 AT2G04910.1 48.750 80 40 1 331 410 15 93 3.14e-21 89.0
Msa1400010 AT1G11820.2 48.864 88 44 1 329 416 379 465 3.21e-21 96.3
Msa1400010 AT4G05430.3 46.512 86 45 1 331 416 48 132 3.80e-21 90.1
Msa1400010 AT1G29380.3 54.023 87 36 3 331 416 148 231 6.87e-21 92.0
Msa1400010 AT4G13600.1 49.425 87 42 2 330 416 60 144 7.23e-21 91.3
Msa1400010 AT1G29380.1 54.023 87 36 3 331 416 148 231 8.65e-21 92.8
Msa1400010 AT1G66870.1 43.182 88 49 1 329 416 24 110 9.12e-21 87.4
Msa1400010 AT1G66870.2 43.182 88 49 1 329 416 51 137 1.47e-20 87.8
Msa1400010 AT1G11820.3 50.000 84 41 1 329 412 379 461 2.09e-20 93.6
Msa1400010 AT1G18650.1 51.163 86 40 2 331 416 21 104 3.09e-20 88.2
Msa1400010 AT5G61130.1 48.837 86 42 2 331 416 21 104 3.41e-20 88.6
Msa1400010 AT4G05430.2 48.750 80 40 1 331 410 48 126 3.86e-20 86.3
Msa1400010 AT3G13560.4 46.667 90 47 1 330 419 361 449 4.02e-20 92.8
Msa1400010 AT3G13560.1 46.667 90 47 1 330 419 361 449 4.02e-20 92.8
Msa1400010 AT3G13560.2 46.667 90 47 1 330 419 361 449 4.02e-20 92.8
Msa1400010 AT3G13560.5 46.667 90 47 1 330 419 361 449 4.02e-20 92.8
Msa1400010 AT3G13560.3 46.667 90 47 1 330 419 361 449 4.02e-20 92.8
Msa1400010 AT1G18650.2 51.163 86 40 2 331 416 21 104 4.30e-20 86.3
Msa1400010 AT1G29380.4 55.556 81 32 3 331 410 148 225 7.63e-20 88.2
Msa1400010 AT1G29380.2 55.556 81 32 3 331 410 148 225 7.63e-20 88.2
Msa1400010 AT2G01630.1 45.455 88 47 1 329 416 358 444 1.29e-19 91.3
Msa1400010 AT1G09460.1 51.163 86 38 3 331 416 137 218 3.62e-19 88.2
Msa1400010 AT3G23770.1 24.060 399 248 16 54 416 92 471 4.32e-19 89.7
Msa1400010 AT5G56590.1 43.678 87 48 1 331 417 369 454 4.49e-19 89.7
Msa1400010 AT5G56590.1 28.571 154 99 4 22 168 49 198 1.03e-11 67.0
Msa1400010 AT2G01630.3 45.783 83 44 1 329 411 358 439 5.51e-19 89.0
Msa1400010 AT5G08000.2 48.889 90 44 2 327 416 36 123 2.05e-18 84.0
Msa1400010 AT5G08000.1 50.000 86 41 2 331 416 21 104 4.15e-18 82.4
Msa1400010 AT4G29360.1 35.433 127 81 1 293 419 353 478 5.87e-18 86.3
Msa1400010 AT4G29360.1 30.464 151 95 3 24 168 52 198 4.43e-11 65.1
Msa1400010 AT4G29360.2 35.433 127 81 1 293 419 363 488 6.00e-18 86.3
Msa1400010 AT4G29360.2 30.464 151 95 3 24 168 62 208 4.74e-11 65.1
Msa1400010 AT5G63225.1 46.429 84 43 2 331 414 25 106 9.09e-18 79.0
Msa1400010 AT5G63230.1 42.045 88 49 2 329 416 24 109 1.69e-17 78.2
Msa1400010 AT1G66852.1 39.773 88 51 2 329 416 24 109 2.37e-17 77.8
Msa1400010 AT3G55430.1 39.535 86 50 2 331 416 366 449 4.53e-17 83.2
Msa1400010 AT2G43670.1 41.667 84 46 2 331 414 35 115 3.71e-16 74.7
Msa1400010 AT5G63250.1 40.541 111 56 4 309 417 27 129 5.06e-16 74.7
Msa1400010 AT4G16165.1 35.227 88 56 1 329 416 24 110 5.42e-16 73.9
Msa1400010 AT5G53610.1 45.349 86 44 2 331 416 28 110 6.95e-16 73.9
Msa1400010 AT4G09090.1 40.230 87 50 2 331 417 31 115 8.79e-16 73.6
Msa1400010 AT2G29735.1 41.111 90 49 3 329 416 29 116 1.05e-15 73.6
Msa1400010 AT4G09462.1 38.636 88 52 2 329 416 27 112 1.61e-15 72.8
Msa1400010 AT4G09465.1 38.636 88 52 2 329 416 29 114 1.63e-15 72.8
Msa1400010 AT4G09464.1 38.636 88 52 2 329 416 29 114 1.91e-15 72.8
Msa1400010 AT4G09467.1 38.636 88 52 2 329 416 29 114 1.91e-15 72.8
Msa1400010 AT4G34480.3 43.023 86 47 2 331 416 253 336 2.99e-15 77.4
Msa1400010 AT4G34480.1 43.023 86 47 2 331 416 364 447 3.09e-15 77.8
Msa1400010 AT4G09466.1 37.500 88 53 2 329 416 29 114 5.67e-15 71.2
Msa1400010 AT1G66250.1 39.326 89 53 1 328 416 365 452 1.05e-14 76.3
Msa1400010 AT2G16230.1 37.864 103 61 3 315 416 349 449 1.89e-14 75.5
Msa1400010 AT5G53600.1 36.559 93 56 2 324 416 22 111 3.08e-14 69.3
Msa1400010 AT1G69295.1 48.611 72 34 3 331 402 21 89 4.26e-14 71.6
Msa1400010 AT3G58100.1 39.773 88 52 1 329 416 39 125 5.64e-14 70.5
Msa1400010 AT1G26450.1 53.226 62 27 2 343 404 32 91 5.72e-14 70.9
Msa1400010 AT1G69295.2 48.611 72 34 3 331 402 17 85 6.06e-14 71.2
Msa1400010 AT3G58100.2 39.773 88 52 1 329 416 115 201 1.11e-13 71.2
Msa1400010 AT2G43660.2 36.957 92 57 1 325 416 29 119 2.21e-13 67.0
Msa1400010 AT2G43660.1 36.957 92 57 1 325 416 28 118 2.24e-13 67.0
Msa1400010 AT5G63240.1 32.039 103 67 2 317 417 26 127 2.25e-13 67.4
Msa1400010 AT5G24318.3 37.736 106 59 4 315 416 214 316 5.59e-13 70.1
Msa1400010 AT5G24318.1 37.736 106 59 4 315 416 353 455 6.04e-13 70.9
Msa1400010 AT1G13830.2 42.105 76 42 2 327 402 31 104 1.19e-12 67.4
Msa1400010 AT1G13830.1 43.836 73 39 2 330 402 20 90 2.13e-12 66.2
Msa1400010 AT1G78520.1 32.000 100 65 1 317 416 18 114 4.18e-12 63.2
Msa1400010 AT5G24318.2 38.835 103 56 4 315 413 353 452 6.13e-12 67.4
Msa1400010 AT3G28250.1 40.000 80 45 2 331 410 3 79 9.55e-12 62.4
Msa1400010 AT2G03505.4 38.750 80 47 2 331 410 21 98 2.29e-11 62.4
Msa1400010 AT2G03505.1 38.750 80 47 2 331 410 21 98 2.29e-11 62.4
Msa1400010 AT2G03505.2 38.750 80 47 2 331 410 21 98 2.29e-11 62.4
Msa1400010 AT2G03505.3 38.750 80 47 2 331 410 21 98 2.56e-11 61.2
Msa1400010 AT2G42930.1 37.113 97 58 3 314 410 23 116 5.13e-11 60.5

Find 91 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACATAAATTCTCCAAAT+TGG 0.147457 tig0022413:-82000 None:intergenic
GTAGTTTGCATTGCAGTTTC+TGG 0.188078 tig0022413:+80304 Msa1400010:intron
TATATTATGTGGACCCAATT+TGG 0.203652 tig0022413:+81986 Msa1400010:three_prime_UTR
TTGTTCTTGAGGGGCAAAAT+AGG 0.257528 tig0022413:-81094 None:intergenic
CTTATGCATTCAATAGTTAC+TGG 0.260747 tig0022413:+81400 Msa1400010:CDS
TCTAAATGAAAAGCATAATA+TGG 0.271737 tig0022413:-82133 None:intergenic
GAGAAGAACCTTGAGATTAT+TGG 0.281894 tig0022413:+80059 Msa1400010:five_prime_UTR
GTTGAGCAATTTCTGGAATT+GGG 0.288063 tig0022413:-80957 None:intergenic
CCTTGCAATGCAATAGAAAA+TGG 0.301168 tig0022413:+81183 Msa1400010:CDS
GGTTGAGCAATTTCTGGAAT+TGG 0.309049 tig0022413:-80958 None:intergenic
GCAAAATAGGGAGAAGAAAT+TGG 0.319946 tig0022413:-81081 None:intergenic
GAACAACATTAAGGTTGTTT+TGG 0.320840 tig0022413:-80558 None:intergenic
CATCTTCTCTTTCTTTGCTC+TGG 0.345679 tig0022413:+80117 Msa1400010:CDS
TTTGAATTTGGTACATTGTA+AGG 0.345996 tig0022413:-81018 None:intergenic
CCTGCTAACTCTCCTCAAAA+TGG 0.348344 tig0022413:+81147 Msa1400010:CDS
CACTTGTTTCACATAGGTTT+AGG 0.352249 tig0022413:-80345 None:intergenic
ATGGTGATGGTTGTTCTTGA+GGG 0.357121 tig0022413:-81104 None:intergenic
TGAAGGTGGTGCTGATTGTT+TGG 0.361551 tig0022413:+81320 Msa1400010:CDS
GCATGAGCAACCAATGTATC+AGG 0.366680 tig0022413:-81375 None:intergenic
ACAAGAAAAGAAAATGAAAA+TGG 0.371125 tig0022413:+80083 Msa1400010:exon
GGTTTGAAGTAGCTGAGTTA+AGG 0.381419 tig0022413:-80681 None:intergenic
TGAAACGTTGCAAGAAGCTT+TGG 0.385008 tig0022413:+81284 Msa1400010:CDS
GCTTGAAGAAACTCAATCAT+AGG 0.387058 tig0022413:-80724 None:intergenic
TGTTCTTGAGGGGCAAAATA+GGG 0.397503 tig0022413:-81093 None:intergenic
AGATCACTTTGGTTGTTGAT+TGG 0.399416 tig0022413:+81925 Msa1400010:three_prime_UTR
ACAGAAGTAGAACAACATTA+AGG 0.399975 tig0022413:-80567 None:intergenic
AGACATAAATTCTCCAAATT+GGG 0.404814 tig0022413:-81999 None:intergenic
TTTCTCTCATACCAAGTTCA+AGG 0.409782 tig0022413:+80761 Msa1400010:CDS
CAATGTTTATGATATTGTTA+TGG 0.410294 tig0022413:-80861 None:intergenic
GTTTGAAGTAGCTGAGTTAA+GGG 0.410988 tig0022413:-80680 None:intergenic
TGGCAAAAGCATAAGAGAAT+TGG 0.415770 tig0022413:+81420 Msa1400010:CDS
GCTTCTTGCAACGTTTCATC+AGG 0.416313 tig0022413:-81279 None:intergenic
TTCTGAACAGGTGCAATTTG+TGG 0.425677 tig0022413:-81228 None:intergenic
ATGTATAAACTTATATTATG+TGG 0.426320 tig0022413:+81975 Msa1400010:three_prime_UTR
TTGCACCTGTTCAGAAACTT+TGG 0.430193 tig0022413:+81235 Msa1400010:CDS
TCTATCATTCCCTCAAAAGA+TGG 0.439369 tig0022413:+80605 Msa1400010:CDS
GATGAAACTATTTGAGCTAA+AGG 0.452012 tig0022413:-81051 None:intergenic
TATCAGCTGAAACTTGGCTT+AGG 0.457511 tig0022413:+80458 Msa1400010:CDS
TTTGCTACTAGCTAGCTAGT+TGG 0.459447 tig0022413:+81949 Msa1400010:three_prime_UTR
TTGCAGGTTTCCTTCACTGT+AGG 0.460667 tig0022413:+81811 Msa1400010:intron
GCAATTTCTGGAATTGGGGT+TGG 0.460853 tig0022413:-80952 None:intergenic
TTTGGTACATTGTAAGGAGC+TGG 0.460933 tig0022413:-81012 None:intergenic
ATTTGAGCTAAAGGTGGTAA+AGG 0.464246 tig0022413:-81042 None:intergenic
TTGGATTATGCTTGTGGTGA+AGG 0.471347 tig0022413:+81303 Msa1400010:CDS
AATGGTGATGGTTGTTCTTG+AGG 0.476899 tig0022413:-81105 None:intergenic
ATTGGTGGAACTTGTGACTT+TGG 0.478733 tig0022413:+81438 Msa1400010:CDS
ACACACCAAAGTTTCTGAAC+AGG 0.478993 tig0022413:-81240 None:intergenic
TTGTTACAATCCTGATACAT+TGG 0.480675 tig0022413:+81365 Msa1400010:CDS
CTATCATTCCCTCAAAAGAT+GGG 0.481726 tig0022413:+80606 Msa1400010:CDS
GTGGAACTTGTGACTTTGGT+GGG 0.482171 tig0022413:+81442 Msa1400010:CDS
TGTTGCTAGAGACCTTGTTA+AGG 0.501164 tig0022413:-80432 None:intergenic
ACATTGTAAGGAGCTGGATA+AGG 0.502108 tig0022413:-81006 None:intergenic
CAAAAGCATAAGAGAATTGG+TGG 0.510123 tig0022413:+81423 Msa1400010:CDS
AAATGTGAAAACCTTGAACT+TGG 0.511512 tig0022413:-80772 None:intergenic
TTGAGCAATTTCTGGAATTG+GGG 0.520319 tig0022413:-80956 None:intergenic
TTGTTCAAAGAAACAGCTAA+AGG 0.528055 tig0022413:-80403 None:intergenic
ATCCTATGTTAAGATCACTT+TGG 0.529038 tig0022413:+81914 Msa1400010:three_prime_UTR
TTCTTGTGCCAATAATCTCA+AGG 0.529385 tig0022413:-80067 None:intergenic
GATACAAACTGTGGTGGTAA+TGG 0.537334 tig0022413:-81123 None:intergenic
AACCAGCAGGGAAATCCAAA+GGG 0.537740 tig0022413:-80981 None:intergenic
TAGGGAGAAGAAATTGGTGG+TGG 0.538044 tig0022413:-81075 None:intergenic
GAACCAGCAGGGAAATCCAA+AGG 0.539386 tig0022413:-80982 None:intergenic
GCTGGATAAGGAGAACCAGC+AGG 0.554077 tig0022413:-80994 None:intergenic
TGTCTTCACTTGTTTCACAT+AGG 0.568580 tig0022413:-80351 None:intergenic
ACAGTCCAAATCAAAAGCAA+TGG 0.570988 tig0022413:-80653 None:intergenic
GTCTTGTTGGCAGAAAGTTG+AGG 0.577252 tig0022413:-80536 None:intergenic
GATTATGCTTGTGGTGAAGG+TGG 0.582581 tig0022413:+81306 Msa1400010:CDS
GGTGGAACTTGTGACTTTGG+TGG 0.584114 tig0022413:+81441 Msa1400010:CDS
AACGTTTCATCAGGAACACT+AGG 0.602336 tig0022413:-81270 None:intergenic
GAAGCTTTGGATTATGCTTG+TGG 0.604890 tig0022413:+81297 Msa1400010:CDS
GGATTGTAACAATTCCCTTG+TGG 0.608596 tig0022413:-81354 None:intergenic
AACCAAAGTGATCTTAACAT+AGG 0.609490 tig0022413:-81916 None:intergenic
TTGGAGATTACTACTCCACA+AGG 0.615549 tig0022413:+81339 Msa1400010:CDS
TGAGAATAAACCTACAGTGA+AGG 0.617438 tig0022413:-81821 None:intergenic
AAAAGAAAATGAAAATGGTG+TGG 0.631353 tig0022413:+80088 Msa1400010:exon
TATCATTCCCTCAAAAGATG+GGG 0.632855 tig0022413:+80607 Msa1400010:CDS
AACTGTGGTGGTAATGGTGA+TGG 0.632946 tig0022413:-81117 None:intergenic
GAAACTATTTGAGCTAAAGG+TGG 0.639226 tig0022413:-81048 None:intergenic
AAACTAATAATAGAAACCAC+AGG 0.643581 tig0022413:-81872 None:intergenic
TGGTGATGGTTGTTCTTGAG+GGG 0.643729 tig0022413:-81103 None:intergenic
AAATAGGGAGAAGAAATTGG+TGG 0.650191 tig0022413:-81078 None:intergenic
GCAATTTGTGGATAATGTGA+AGG 0.661579 tig0022413:-81216 None:intergenic
TGTGGATAATGTGAAGGTGA+AGG 0.662098 tig0022413:-81210 None:intergenic
TGCATGAAGAGTTGTTCCTG+TGG 0.663059 tig0022413:+81856 Msa1400010:three_prime_UTR
GAACAATGAAGACCTTAACA+AGG 0.665682 tig0022413:+80420 Msa1400010:CDS
CTGGATAAGGAGAACCAGCA+GGG 0.668605 tig0022413:-80993 None:intergenic
GCAGGAGATACAAACTGTGG+TGG 0.675527 tig0022413:-81129 None:intergenic
ACCAGCAGGGAAATCCAAAG+GGG 0.725497 tig0022413:-80980 None:intergenic
TGGAGATTACTACTCCACAA+GGG 0.734669 tig0022413:+81340 Msa1400010:CDS
CCAGCAGGGAAATCCAAAGG+GGG 0.739134 tig0022413:-80979 None:intergenic
TTAGCAGGAGATACAAACTG+TGG 0.759496 tig0022413:-81132 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTATAAACTTATATTATG+TGG + tig0022413:81975-81994 Msa1400010:three_prime_UTR 15.0%
!!! ATGTCTTAATTTTTGTTATT+AGG + tig0022413:82016-82035 Msa1400010:three_prime_UTR 15.0%
!!! TAATTTTTGTTATTAGGATT+TGG + tig0022413:82022-82041 Msa1400010:three_prime_UTR 15.0%
!! ATGTATAAACTTATATTATG+TGG + tig0022413:81975-81994 Msa1400010:three_prime_UTR 15.0%
!!! ATGTCTTAATTTTTGTTATT+AGG + tig0022413:82016-82035 Msa1400010:three_prime_UTR 15.0%
!!! TAATTTTTGTTATTAGGATT+TGG + tig0022413:82022-82041 Msa1400010:three_prime_UTR 15.0%
!! ACAAGAAAAGAAAATGAAAA+TGG + tig0022413:80083-80102 Msa1400010:exon 20.0%
!!! GTTTTAACATTTTCAAATCA+TGG - tig0022413:80705-80724 None:intergenic 20.0%
!! CAATGTTTATGATATTGTTA+TGG - tig0022413:80864-80883 None:intergenic 20.0%
!! TAAATTAATAAGCACTTACT+TGG - tig0022413:81492-81511 None:intergenic 20.0%
!! AAGTAAGTGCTTATTAATTT+AGG + tig0022413:81491-81510 Msa1400010:intron 20.0%
!! TGTTAATTAATAATGAGTGA+TGG + tig0022413:81542-81561 Msa1400010:intron 20.0%
!!! AATTAATTAGCTGTTTTAGA+TGG - tig0022413:81726-81745 None:intergenic 20.0%
!! TTACATTAATAATGGTGAAT+TGG + tig0022413:81777-81796 Msa1400010:intron 20.0%
!!! TCTAAATGAAAAGCATAATA+TGG - tig0022413:82136-82155 None:intergenic 20.0%
!! ACAAGAAAAGAAAATGAAAA+TGG + tig0022413:80083-80102 Msa1400010:exon 20.0%
!!! GTTTTAACATTTTCAAATCA+TGG - tig0022413:80705-80724 None:intergenic 20.0%
!! CAATGTTTATGATATTGTTA+TGG - tig0022413:80864-80883 None:intergenic 20.0%
!! TAAATTAATAAGCACTTACT+TGG - tig0022413:81492-81511 None:intergenic 20.0%
!! AAGTAAGTGCTTATTAATTT+AGG + tig0022413:81491-81510 Msa1400010:intron 20.0%
!! TGTTAATTAATAATGAGTGA+TGG + tig0022413:81542-81561 Msa1400010:intron 20.0%
!!! AATTAATTAGCTGTTTTAGA+TGG - tig0022413:81726-81745 None:intergenic 20.0%
!! TTACATTAATAATGGTGAAT+TGG + tig0022413:81777-81796 Msa1400010:intron 20.0%
!!! TCTAAATGAAAAGCATAATA+TGG - tig0022413:82136-82155 None:intergenic 20.0%
! AAAAGAAAATGAAAATGGTG+TGG + tig0022413:80088-80107 Msa1400010:exon 25.0%
!! TTTTTGATGAAGGGTAATAT+GGG - tig0022413:80495-80514 None:intergenic 25.0%
!! ATTTTTGATGAAGGGTAATA+TGG - tig0022413:80496-80515 None:intergenic 25.0%
! TTAGAATCCATGAAAAAACT+TGG + tig0022413:80829-80848 Msa1400010:CDS 25.0%
!!! GAAAAATCCAAGTTTTTTCA+TGG - tig0022413:80839-80858 None:intergenic 25.0%
!! TAGTGATTTTTCTCTCTTTT+GGG - tig0022413:80897-80916 None:intergenic 25.0%
! TTTGAATTTGGTACATTGTA+AGG - tig0022413:81021-81040 None:intergenic 25.0%
! TATAGTTATTGTACATAGCA+GGG - tig0022413:81656-81675 None:intergenic 25.0%
! TTATAGTTATTGTACATAGC+AGG - tig0022413:81657-81676 None:intergenic 25.0%
! ATAAAGTATGTAACTACCAA+AGG + tig0022413:81673-81692 Msa1400010:intron 25.0%
! AAACTAATAATAGAAACCAC+AGG - tig0022413:81875-81894 None:intergenic 25.0%
! AGACATAAATTCTCCAAATT+GGG - tig0022413:82002-82021 None:intergenic 25.0%
! AAGACATAAATTCTCCAAAT+TGG - tig0022413:82003-82022 None:intergenic 25.0%
!! TCACTAATCAGAATTTTGAA+GGG - tig0022413:82081-82100 None:intergenic 25.0%
!! ATCACTAATCAGAATTTTGA+AGG - tig0022413:82082-82101 None:intergenic 25.0%
! AAAAGAAAATGAAAATGGTG+TGG + tig0022413:80088-80107 Msa1400010:exon 25.0%
!! TTTTTGATGAAGGGTAATAT+GGG - tig0022413:80495-80514 None:intergenic 25.0%
!! ATTTTTGATGAAGGGTAATA+TGG - tig0022413:80496-80515 None:intergenic 25.0%
! TTAGAATCCATGAAAAAACT+TGG + tig0022413:80829-80848 Msa1400010:CDS 25.0%
!!! GAAAAATCCAAGTTTTTTCA+TGG - tig0022413:80839-80858 None:intergenic 25.0%
!! TAGTGATTTTTCTCTCTTTT+GGG - tig0022413:80897-80916 None:intergenic 25.0%
! TTTGAATTTGGTACATTGTA+AGG - tig0022413:81021-81040 None:intergenic 25.0%
! TATAGTTATTGTACATAGCA+GGG - tig0022413:81656-81675 None:intergenic 25.0%
! TTATAGTTATTGTACATAGC+AGG - tig0022413:81657-81676 None:intergenic 25.0%
! ATAAAGTATGTAACTACCAA+AGG + tig0022413:81673-81692 Msa1400010:intron 25.0%
! AAACTAATAATAGAAACCAC+AGG - tig0022413:81875-81894 None:intergenic 25.0%
! AGACATAAATTCTCCAAATT+GGG - tig0022413:82002-82021 None:intergenic 25.0%
! AAGACATAAATTCTCCAAAT+TGG - tig0022413:82003-82022 None:intergenic 25.0%
!! TCACTAATCAGAATTTTGAA+GGG - tig0022413:82081-82100 None:intergenic 25.0%
!! ATCACTAATCAGAATTTTGA+AGG - tig0022413:82082-82101 None:intergenic 25.0%
TTGTTCAAAGAAACAGCTAA+AGG - tig0022413:80406-80425 None:intergenic 30.0%
! ACATTTTATCAGCTGAAACT+TGG + tig0022413:80452-80471 Msa1400010:CDS 30.0%
!!! TGTTGGTGATTTTTGATGAA+GGG - tig0022413:80504-80523 None:intergenic 30.0%
!!! ATGTTGGTGATTTTTGATGA+AGG - tig0022413:80505-80524 None:intergenic 30.0%
TCAAAAATCACCAACATTGT+TGG + tig0022413:80508-80527 Msa1400010:CDS 30.0%
! GAACAACATTAAGGTTGTTT+TGG - tig0022413:80561-80580 None:intergenic 30.0%
ACAGAAGTAGAACAACATTA+AGG - tig0022413:80570-80589 None:intergenic 30.0%
!! AGTTTCCATTGCTTTTGATT+TGG + tig0022413:80648-80667 Msa1400010:CDS 30.0%
AAATGTGAAAACCTTGAACT+TGG - tig0022413:80775-80794 None:intergenic 30.0%
!!! TTTTCTCTCTTTTGGGATAA+TGG - tig0022413:80890-80909 None:intergenic 30.0%
! GTAGTGATTTTTCTCTCTTT+TGG - tig0022413:80898-80917 None:intergenic 30.0%
GATGAAACTATTTGAGCTAA+AGG - tig0022413:81054-81073 None:intergenic 30.0%
TTGTTACAATCCTGATACAT+TGG + tig0022413:81365-81384 Msa1400010:CDS 30.0%
CTTATGCATTCAATAGTTAC+TGG + tig0022413:81400-81419 Msa1400010:CDS 30.0%
! AGTTAGTTACACAGTTTGTT+AGG - tig0022413:81699-81718 None:intergenic 30.0%
!! ATTGGTTATTGTTTTGTTGC+AGG + tig0022413:81795-81814 Msa1400010:intron 30.0%
ATCCTATGTTAAGATCACTT+TGG + tig0022413:81914-81933 Msa1400010:three_prime_UTR 30.0%
AACCAAAGTGATCTTAACAT+AGG - tig0022413:81919-81938 None:intergenic 30.0%
TATATTATGTGGACCCAATT+TGG + tig0022413:81986-82005 Msa1400010:three_prime_UTR 30.0%
TTGTTCAAAGAAACAGCTAA+AGG - tig0022413:80406-80425 None:intergenic 30.0%
! ACATTTTATCAGCTGAAACT+TGG + tig0022413:80452-80471 Msa1400010:CDS 30.0%
!!! TGTTGGTGATTTTTGATGAA+GGG - tig0022413:80504-80523 None:intergenic 30.0%
!!! ATGTTGGTGATTTTTGATGA+AGG - tig0022413:80505-80524 None:intergenic 30.0%
TCAAAAATCACCAACATTGT+TGG + tig0022413:80508-80527 Msa1400010:CDS 30.0%
! GAACAACATTAAGGTTGTTT+TGG - tig0022413:80561-80580 None:intergenic 30.0%
ACAGAAGTAGAACAACATTA+AGG - tig0022413:80570-80589 None:intergenic 30.0%
!! AGTTTCCATTGCTTTTGATT+TGG + tig0022413:80648-80667 Msa1400010:CDS 30.0%
AAATGTGAAAACCTTGAACT+TGG - tig0022413:80775-80794 None:intergenic 30.0%
!!! TTTTCTCTCTTTTGGGATAA+TGG - tig0022413:80890-80909 None:intergenic 30.0%
! GTAGTGATTTTTCTCTCTTT+TGG - tig0022413:80898-80917 None:intergenic 30.0%
GATGAAACTATTTGAGCTAA+AGG - tig0022413:81054-81073 None:intergenic 30.0%
TTGTTACAATCCTGATACAT+TGG + tig0022413:81365-81384 Msa1400010:CDS 30.0%
CTTATGCATTCAATAGTTAC+TGG + tig0022413:81400-81419 Msa1400010:CDS 30.0%
! AGTTAGTTACACAGTTTGTT+AGG - tig0022413:81699-81718 None:intergenic 30.0%
!! ATTGGTTATTGTTTTGTTGC+AGG + tig0022413:81795-81814 Msa1400010:intron 30.0%
ATCCTATGTTAAGATCACTT+TGG + tig0022413:81914-81933 Msa1400010:three_prime_UTR 30.0%
AACCAAAGTGATCTTAACAT+AGG - tig0022413:81919-81938 None:intergenic 30.0%
TATATTATGTGGACCCAATT+TGG + tig0022413:81986-82005 Msa1400010:three_prime_UTR 30.0%
GAGAAGAACCTTGAGATTAT+TGG + tig0022413:80059-80078 Msa1400010:five_prime_UTR 35.0%
TTCTTGTGCCAATAATCTCA+AGG - tig0022413:80070-80089 None:intergenic 35.0%
CACTTGTTTCACATAGGTTT+AGG - tig0022413:80348-80367 None:intergenic 35.0%
TGTCTTCACTTGTTTCACAT+AGG - tig0022413:80354-80373 None:intergenic 35.0%
GAACAATGAAGACCTTAACA+AGG + tig0022413:80420-80439 Msa1400010:CDS 35.0%
! TGAGGTTTTTCCAACAATGT+TGG - tig0022413:80521-80540 None:intergenic 35.0%
!!! TAAGGTTGTTTTGGTCTTGT+TGG - tig0022413:80552-80571 None:intergenic 35.0%
TCTATCATTCCCTCAAAAGA+TGG + tig0022413:80605-80624 Msa1400010:CDS 35.0%
CTATCATTCCCTCAAAAGAT+GGG + tig0022413:80606-80625 Msa1400010:CDS 35.0%
TATCATTCCCTCAAAAGATG+GGG + tig0022413:80607-80626 Msa1400010:CDS 35.0%
! TTCTAAACCCCATCTTTTGA+GGG - tig0022413:80617-80636 None:intergenic 35.0%
ACAGTCCAAATCAAAAGCAA+TGG - tig0022413:80656-80675 None:intergenic 35.0%
! GTTTGAAGTAGCTGAGTTAA+GGG - tig0022413:80683-80702 None:intergenic 35.0%
GCTTGAAGAAACTCAATCAT+AGG - tig0022413:80727-80746 None:intergenic 35.0%
TTTCTCTCATACCAAGTTCA+AGG + tig0022413:80761-80780 Msa1400010:CDS 35.0%
!!! GGTTGGTTTTATAGGAATTG+TGG - tig0022413:80938-80957 None:intergenic 35.0%
!! TTGAGCAATTTCTGGAATTG+GGG - tig0022413:80959-80978 None:intergenic 35.0%
!! GTTGAGCAATTTCTGGAATT+GGG - tig0022413:80960-80979 None:intergenic 35.0%
!!! GGTGGTAAAGGTTTTGAATT+TGG - tig0022413:81033-81052 None:intergenic 35.0%
ATTTGAGCTAAAGGTGGTAA+AGG - tig0022413:81045-81064 None:intergenic 35.0%
GAAACTATTTGAGCTAAAGG+TGG - tig0022413:81051-81070 None:intergenic 35.0%
AAATAGGGAGAAGAAATTGG+TGG - tig0022413:81081-81100 None:intergenic 35.0%
GCAAAATAGGGAGAAGAAAT+TGG - tig0022413:81084-81103 None:intergenic 35.0%
! TTTTCTATTGCATTGCAAGG+TGG - tig0022413:81183-81202 None:intergenic 35.0%
! CCATTTTCTATTGCATTGCA+AGG - tig0022413:81186-81205 None:intergenic 35.0%
CCTTGCAATGCAATAGAAAA+TGG + tig0022413:81183-81202 Msa1400010:CDS 35.0%
GCAATTTGTGGATAATGTGA+AGG - tig0022413:81219-81238 None:intergenic 35.0%
! TGGCAAAAGCATAAGAGAAT+TGG + tig0022413:81420-81439 Msa1400010:CDS 35.0%
! CAAAAGCATAAGAGAATTGG+TGG + tig0022413:81423-81442 Msa1400010:CDS 35.0%
TGAGAATAAACCTACAGTGA+AGG - tig0022413:81824-81843 None:intergenic 35.0%
AGATCACTTTGGTTGTTGAT+TGG + tig0022413:81925-81944 Msa1400010:three_prime_UTR 35.0%
GAGAAGAACCTTGAGATTAT+TGG + tig0022413:80059-80078 Msa1400010:five_prime_UTR 35.0%
TTCTTGTGCCAATAATCTCA+AGG - tig0022413:80070-80089 None:intergenic 35.0%
CACTTGTTTCACATAGGTTT+AGG - tig0022413:80348-80367 None:intergenic 35.0%
TGTCTTCACTTGTTTCACAT+AGG - tig0022413:80354-80373 None:intergenic 35.0%
GAACAATGAAGACCTTAACA+AGG + tig0022413:80420-80439 Msa1400010:CDS 35.0%
! TGAGGTTTTTCCAACAATGT+TGG - tig0022413:80521-80540 None:intergenic 35.0%
!!! TAAGGTTGTTTTGGTCTTGT+TGG - tig0022413:80552-80571 None:intergenic 35.0%
TCTATCATTCCCTCAAAAGA+TGG + tig0022413:80605-80624 Msa1400010:CDS 35.0%
CTATCATTCCCTCAAAAGAT+GGG + tig0022413:80606-80625 Msa1400010:CDS 35.0%
TATCATTCCCTCAAAAGATG+GGG + tig0022413:80607-80626 Msa1400010:CDS 35.0%
! TTCTAAACCCCATCTTTTGA+GGG - tig0022413:80617-80636 None:intergenic 35.0%
ACAGTCCAAATCAAAAGCAA+TGG - tig0022413:80656-80675 None:intergenic 35.0%
! GTTTGAAGTAGCTGAGTTAA+GGG - tig0022413:80683-80702 None:intergenic 35.0%
GCTTGAAGAAACTCAATCAT+AGG - tig0022413:80727-80746 None:intergenic 35.0%
TTTCTCTCATACCAAGTTCA+AGG + tig0022413:80761-80780 Msa1400010:CDS 35.0%
!!! GGTTGGTTTTATAGGAATTG+TGG - tig0022413:80938-80957 None:intergenic 35.0%
!! TTGAGCAATTTCTGGAATTG+GGG - tig0022413:80959-80978 None:intergenic 35.0%
!! GTTGAGCAATTTCTGGAATT+GGG - tig0022413:80960-80979 None:intergenic 35.0%
!!! GGTGGTAAAGGTTTTGAATT+TGG - tig0022413:81033-81052 None:intergenic 35.0%
ATTTGAGCTAAAGGTGGTAA+AGG - tig0022413:81045-81064 None:intergenic 35.0%
GAAACTATTTGAGCTAAAGG+TGG - tig0022413:81051-81070 None:intergenic 35.0%
AAATAGGGAGAAGAAATTGG+TGG - tig0022413:81081-81100 None:intergenic 35.0%
GCAAAATAGGGAGAAGAAAT+TGG - tig0022413:81084-81103 None:intergenic 35.0%
! TTTTCTATTGCATTGCAAGG+TGG - tig0022413:81183-81202 None:intergenic 35.0%
! CCATTTTCTATTGCATTGCA+AGG - tig0022413:81186-81205 None:intergenic 35.0%
CCTTGCAATGCAATAGAAAA+TGG + tig0022413:81183-81202 Msa1400010:CDS 35.0%
GCAATTTGTGGATAATGTGA+AGG - tig0022413:81219-81238 None:intergenic 35.0%
! TGGCAAAAGCATAAGAGAAT+TGG + tig0022413:81420-81439 Msa1400010:CDS 35.0%
! CAAAAGCATAAGAGAATTGG+TGG + tig0022413:81423-81442 Msa1400010:CDS 35.0%
TGAGAATAAACCTACAGTGA+AGG - tig0022413:81824-81843 None:intergenic 35.0%
AGATCACTTTGGTTGTTGAT+TGG + tig0022413:81925-81944 Msa1400010:three_prime_UTR 35.0%
CATCTTCTCTTTCTTTGCTC+TGG + tig0022413:80117-80136 Msa1400010:CDS 40.0%
! GTAGTTTGCATTGCAGTTTC+TGG + tig0022413:80304-80323 Msa1400010:intron 40.0%
TGTTGCTAGAGACCTTGTTA+AGG - tig0022413:80435-80454 None:intergenic 40.0%
TATCAGCTGAAACTTGGCTT+AGG + tig0022413:80458-80477 Msa1400010:CDS 40.0%
!! GTTCTAAACCCCATCTTTTG+AGG - tig0022413:80618-80637 None:intergenic 40.0%
! GGTTTGAAGTAGCTGAGTTA+AGG - tig0022413:80684-80703 None:intergenic 40.0%
!!! GGAATTGGGGTTGGTTTTAT+AGG - tig0022413:80946-80965 None:intergenic 40.0%
!! GGTTGAGCAATTTCTGGAAT+TGG - tig0022413:80961-80980 None:intergenic 40.0%
ACATTGTAAGGAGCTGGATA+AGG - tig0022413:81009-81028 None:intergenic 40.0%
TTTGGTACATTGTAAGGAGC+TGG - tig0022413:81015-81034 None:intergenic 40.0%
TGTTCTTGAGGGGCAAAATA+GGG - tig0022413:81096-81115 None:intergenic 40.0%
TTGTTCTTGAGGGGCAAAAT+AGG - tig0022413:81097-81116 None:intergenic 40.0%
!! ATGGTGATGGTTGTTCTTGA+GGG - tig0022413:81107-81126 None:intergenic 40.0%
!! AATGGTGATGGTTGTTCTTG+AGG - tig0022413:81108-81127 None:intergenic 40.0%
GATACAAACTGTGGTGGTAA+TGG - tig0022413:81126-81145 None:intergenic 40.0%
TTAGCAGGAGATACAAACTG+TGG - tig0022413:81135-81154 None:intergenic 40.0%
! GGACAAGTGAAACCATTTTG+AGG - tig0022413:81162-81181 None:intergenic 40.0%
TGTGGATAATGTGAAGGTGA+AGG - tig0022413:81213-81232 None:intergenic 40.0%
TTCTGAACAGGTGCAATTTG+TGG - tig0022413:81231-81250 None:intergenic 40.0%
TTGCACCTGTTCAGAAACTT+TGG + tig0022413:81235-81254 Msa1400010:CDS 40.0%
ACACACCAAAGTTTCTGAAC+AGG - tig0022413:81243-81262 None:intergenic 40.0%
AACGTTTCATCAGGAACACT+AGG - tig0022413:81273-81292 None:intergenic 40.0%
TGAAACGTTGCAAGAAGCTT+TGG + tig0022413:81284-81303 Msa1400010:CDS 40.0%
!! GAAGCTTTGGATTATGCTTG+TGG + tig0022413:81297-81316 Msa1400010:CDS 40.0%
TTGGATTATGCTTGTGGTGA+AGG + tig0022413:81303-81322 Msa1400010:CDS 40.0%
TTGGAGATTACTACTCCACA+AGG + tig0022413:81339-81358 Msa1400010:CDS 40.0%
TGGAGATTACTACTCCACAA+GGG + tig0022413:81340-81359 Msa1400010:CDS 40.0%
GGATTGTAACAATTCCCTTG+TGG - tig0022413:81357-81376 None:intergenic 40.0%
ATTGGTGGAACTTGTGACTT+TGG + tig0022413:81438-81457 Msa1400010:CDS 40.0%
AGTGATGGTTGTGTTGTGAA+TGG + tig0022413:81557-81576 Msa1400010:intron 40.0%
!! TACACAGTTTGTTAGGCCTT+TGG - tig0022413:81692-81711 None:intergenic 40.0%
TTTGCTACTAGCTAGCTAGT+TGG + tig0022413:81949-81968 Msa1400010:three_prime_UTR 40.0%
CATCTTCTCTTTCTTTGCTC+TGG + tig0022413:80117-80136 Msa1400010:CDS 40.0%
! GTAGTTTGCATTGCAGTTTC+TGG + tig0022413:80304-80323 Msa1400010:intron 40.0%
TGTTGCTAGAGACCTTGTTA+AGG - tig0022413:80435-80454 None:intergenic 40.0%
TATCAGCTGAAACTTGGCTT+AGG + tig0022413:80458-80477 Msa1400010:CDS 40.0%
!! GTTCTAAACCCCATCTTTTG+AGG - tig0022413:80618-80637 None:intergenic 40.0%
! GGTTTGAAGTAGCTGAGTTA+AGG - tig0022413:80684-80703 None:intergenic 40.0%
!!! GGAATTGGGGTTGGTTTTAT+AGG - tig0022413:80946-80965 None:intergenic 40.0%
!! GGTTGAGCAATTTCTGGAAT+TGG - tig0022413:80961-80980 None:intergenic 40.0%
ACATTGTAAGGAGCTGGATA+AGG - tig0022413:81009-81028 None:intergenic 40.0%
TTTGGTACATTGTAAGGAGC+TGG - tig0022413:81015-81034 None:intergenic 40.0%
TGTTCTTGAGGGGCAAAATA+GGG - tig0022413:81096-81115 None:intergenic 40.0%
TTGTTCTTGAGGGGCAAAAT+AGG - tig0022413:81097-81116 None:intergenic 40.0%
!! ATGGTGATGGTTGTTCTTGA+GGG - tig0022413:81107-81126 None:intergenic 40.0%
!! AATGGTGATGGTTGTTCTTG+AGG - tig0022413:81108-81127 None:intergenic 40.0%
GATACAAACTGTGGTGGTAA+TGG - tig0022413:81126-81145 None:intergenic 40.0%
TTAGCAGGAGATACAAACTG+TGG - tig0022413:81135-81154 None:intergenic 40.0%
! GGACAAGTGAAACCATTTTG+AGG - tig0022413:81162-81181 None:intergenic 40.0%
TGTGGATAATGTGAAGGTGA+AGG - tig0022413:81213-81232 None:intergenic 40.0%
TTCTGAACAGGTGCAATTTG+TGG - tig0022413:81231-81250 None:intergenic 40.0%
TTGCACCTGTTCAGAAACTT+TGG + tig0022413:81235-81254 Msa1400010:CDS 40.0%
ACACACCAAAGTTTCTGAAC+AGG - tig0022413:81243-81262 None:intergenic 40.0%
AACGTTTCATCAGGAACACT+AGG - tig0022413:81273-81292 None:intergenic 40.0%
TGAAACGTTGCAAGAAGCTT+TGG + tig0022413:81284-81303 Msa1400010:CDS 40.0%
!! GAAGCTTTGGATTATGCTTG+TGG + tig0022413:81297-81316 Msa1400010:CDS 40.0%
TTGGATTATGCTTGTGGTGA+AGG + tig0022413:81303-81322 Msa1400010:CDS 40.0%
TTGGAGATTACTACTCCACA+AGG + tig0022413:81339-81358 Msa1400010:CDS 40.0%
TGGAGATTACTACTCCACAA+GGG + tig0022413:81340-81359 Msa1400010:CDS 40.0%
GGATTGTAACAATTCCCTTG+TGG - tig0022413:81357-81376 None:intergenic 40.0%
ATTGGTGGAACTTGTGACTT+TGG + tig0022413:81438-81457 Msa1400010:CDS 40.0%
AGTGATGGTTGTGTTGTGAA+TGG + tig0022413:81557-81576 Msa1400010:intron 40.0%
!! TACACAGTTTGTTAGGCCTT+TGG - tig0022413:81692-81711 None:intergenic 40.0%
TTTGCTACTAGCTAGCTAGT+TGG + tig0022413:81949-81968 Msa1400010:three_prime_UTR 40.0%
! GTCTTGTTGGCAGAAAGTTG+AGG - tig0022413:80539-80558 None:intergenic 45.0%
!! GCAATTTCTGGAATTGGGGT+TGG - tig0022413:80955-80974 None:intergenic 45.0%
AAAGGGGGTTGAGCAATTTC+TGG - tig0022413:80967-80986 None:intergenic 45.0%
AGAAATTGCTCAACCCCCTT+TGG + tig0022413:80966-80985 Msa1400010:CDS 45.0%
AACCAGCAGGGAAATCCAAA+GGG - tig0022413:80984-81003 None:intergenic 45.0%
TAGGGAGAAGAAATTGGTGG+TGG - tig0022413:81078-81097 None:intergenic 45.0%
!! TGGTGATGGTTGTTCTTGAG+GGG - tig0022413:81106-81125 None:intergenic 45.0%
!! AACTGTGGTGGTAATGGTGA+TGG - tig0022413:81120-81139 None:intergenic 45.0%
!! CCATTTTGAGGAGAGTTAGC+AGG - tig0022413:81150-81169 None:intergenic 45.0%
CCTGCTAACTCTCCTCAAAA+TGG + tig0022413:81147-81166 Msa1400010:CDS 45.0%
GCTTCTTGCAACGTTTCATC+AGG - tig0022413:81282-81301 None:intergenic 45.0%
GATTATGCTTGTGGTGAAGG+TGG + tig0022413:81306-81325 Msa1400010:CDS 45.0%
!! TGAAGGTGGTGCTGATTGTT+TGG + tig0022413:81320-81339 Msa1400010:CDS 45.0%
GCATGAGCAACCAATGTATC+AGG - tig0022413:81378-81397 None:intergenic 45.0%
GTGGAACTTGTGACTTTGGT+GGG + tig0022413:81442-81461 Msa1400010:CDS 45.0%
TTGCAGGTTTCCTTCACTGT+AGG + tig0022413:81811-81830 Msa1400010:intron 45.0%
TGCATGAAGAGTTGTTCCTG+TGG + tig0022413:81856-81875 Msa1400010:three_prime_UTR 45.0%
! GTCTTGTTGGCAGAAAGTTG+AGG - tig0022413:80539-80558 None:intergenic 45.0%
!! GCAATTTCTGGAATTGGGGT+TGG - tig0022413:80955-80974 None:intergenic 45.0%
AAAGGGGGTTGAGCAATTTC+TGG - tig0022413:80967-80986 None:intergenic 45.0%
AGAAATTGCTCAACCCCCTT+TGG + tig0022413:80966-80985 Msa1400010:CDS 45.0%
AACCAGCAGGGAAATCCAAA+GGG - tig0022413:80984-81003 None:intergenic 45.0%
TAGGGAGAAGAAATTGGTGG+TGG - tig0022413:81078-81097 None:intergenic 45.0%
!! TGGTGATGGTTGTTCTTGAG+GGG - tig0022413:81106-81125 None:intergenic 45.0%
!! AACTGTGGTGGTAATGGTGA+TGG - tig0022413:81120-81139 None:intergenic 45.0%
!! CCATTTTGAGGAGAGTTAGC+AGG - tig0022413:81150-81169 None:intergenic 45.0%
CCTGCTAACTCTCCTCAAAA+TGG + tig0022413:81147-81166 Msa1400010:CDS 45.0%
GCTTCTTGCAACGTTTCATC+AGG - tig0022413:81282-81301 None:intergenic 45.0%
GATTATGCTTGTGGTGAAGG+TGG + tig0022413:81306-81325 Msa1400010:CDS 45.0%
!! TGAAGGTGGTGCTGATTGTT+TGG + tig0022413:81320-81339 Msa1400010:CDS 45.0%
GCATGAGCAACCAATGTATC+AGG - tig0022413:81378-81397 None:intergenic 45.0%
GTGGAACTTGTGACTTTGGT+GGG + tig0022413:81442-81461 Msa1400010:CDS 45.0%
TTGCAGGTTTCCTTCACTGT+AGG + tig0022413:81811-81830 Msa1400010:intron 45.0%
TGCATGAAGAGTTGTTCCTG+TGG + tig0022413:81856-81875 Msa1400010:three_prime_UTR 45.0%
!! TTATTTATTTACATTAATAA+TGG + tig0022413:81769-81788 Msa1400010:intron 5.0%
!! TTATTTATTTACATTAATAA+TGG + tig0022413:81769-81788 Msa1400010:intron 5.0%
ACCAGCAGGGAAATCCAAAG+GGG - tig0022413:80983-81002 None:intergenic 50.0%
GAACCAGCAGGGAAATCCAA+AGG - tig0022413:80985-81004 None:intergenic 50.0%
CTGGATAAGGAGAACCAGCA+GGG - tig0022413:80996-81015 None:intergenic 50.0%
GCAGGAGATACAAACTGTGG+TGG - tig0022413:81132-81151 None:intergenic 50.0%
GGTGGAACTTGTGACTTTGG+TGG + tig0022413:81441-81460 Msa1400010:CDS 50.0%
ACCAGCAGGGAAATCCAAAG+GGG - tig0022413:80983-81002 None:intergenic 50.0%
GAACCAGCAGGGAAATCCAA+AGG - tig0022413:80985-81004 None:intergenic 50.0%
CTGGATAAGGAGAACCAGCA+GGG - tig0022413:80996-81015 None:intergenic 50.0%
GCAGGAGATACAAACTGTGG+TGG - tig0022413:81132-81151 None:intergenic 50.0%
GGTGGAACTTGTGACTTTGG+TGG + tig0022413:81441-81460 Msa1400010:CDS 50.0%
CCAGCAGGGAAATCCAAAGG+GGG - tig0022413:80982-81001 None:intergenic 55.0%
GCTGGATAAGGAGAACCAGC+AGG - tig0022413:80997-81016 None:intergenic 55.0%
CCAGCAGGGAAATCCAAAGG+GGG - tig0022413:80982-81001 None:intergenic 55.0%
GCTGGATAAGGAGAACCAGC+AGG - tig0022413:80997-81016 None:intergenic 55.0%
CCCCCTTTGGATTTCCCTGC+TGG + tig0022413:80979-80998 Msa1400010:CDS 60.0%
CCCCCTTTGGATTTCCCTGC+TGG + tig0022413:80979-80998 Msa1400010:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0022413 gene 80032 82161 80032 ID=Msa1400010;Name=Msa1400010
tig0022413 mRNA 80032 82161 80032 ID=Msa1400010-mRNA-1;Parent=Msa1400010;Name=Msa1400010-mRNA-1;_AED=0.01;_eAED=0.01;_QI=64|1|1|1|1|1|3|313|419
tig0022413 exon 80032 80138 80032 ID=Msa1400010-mRNA-1:exon:17507;Parent=Msa1400010-mRNA-1
tig0022413 exon 80308 81492 80308 ID=Msa1400010-mRNA-1:exon:17508;Parent=Msa1400010-mRNA-1
tig0022413 exon 81817 82161 81817 ID=Msa1400010-mRNA-1:exon:17509;Parent=Msa1400010-mRNA-1
tig0022413 five_prime_UTR 80032 80095 80032 ID=Msa1400010-mRNA-1:five_prime_utr;Parent=Msa1400010-mRNA-1
tig0022413 CDS 80096 80138 80096 ID=Msa1400010-mRNA-1:cds;Parent=Msa1400010-mRNA-1
tig0022413 CDS 80308 81492 80308 ID=Msa1400010-mRNA-1:cds;Parent=Msa1400010-mRNA-1
tig0022413 CDS 81817 81848 81817 ID=Msa1400010-mRNA-1:cds;Parent=Msa1400010-mRNA-1
tig0022413 three_prime_UTR 81849 82161 81849 ID=Msa1400010-mRNA-1:three_prime_utr;Parent=Msa1400010-mRNA-1
Gene Sequence

>Msa1400010

ATGAAAATGGTGTGGCTATTTCATCTTCTCTTTCTTTGCTCTGTTTGCATTGCAGTTTCTGGTCAACAAAACATTGAGTTCCTAAACCTATGTGAAACAAGTGAAGACATTGCAACATCTTCATCTCACAGTGAGTTTCCTTTAGCTGTTTCTTTGAACAATGAAGACCTTAACAAGGTCTCTAGCAACATTTTATCAGCTGAAACTTGGCTTAGGTTAAACATTTTCCCATATTACCCTTCATCAAAAATCACCAACATTGTTGGAAAAACCTCAACTTTCTGCCAACAAGACCAAAACAACCTTAATGTTGTTCTACTTCTGTCTTCTTTGAAAAATCTCTATCATTCCCTCAAAAGATGGGGTTTAGAACAAGACATCAAAGTTTCCATTGCTTTTGATTTGGACTGTTTTACCCTTAACTCAGCTACTTCAAACCATGATTTGAAAATGTTAAAACCTATGATTGAGTTTCTTCAAGCTGTTAACTCAACATTTTCTCTCATACCAAGTTCAAGGTTTTCACATTTTTCTGATAAAAGTTTAAACTTTGTGTCTTCTCAATTAGAATCCATGAAAAAACTTGGATTTTTCAACCATAACAATATCATAAACATTGCTACCATTATCCCAAAAGAGAGAAAAATCACTACTAGAAAACTTTCAGTTACCACAATTCCTATAAAACCAACCCCAATTCCAGAAATTGCTCAACCCCCTTTGGATTTCCCTGCTGGTTCTCCTTATCCAGCTCCTTACAATGTACCAAATTCAAAACCTTTACCACCTTTAGCTCAAATAGTTTCATCTCCACCACCAATTTCTTCTCCCTATTTTGCCCCTCAAGAACAACCATCACCATTACCACCACAGTTTGTATCTCCTGCTAACTCTCCTCAAAATGGTTTCACTTGTCCACCTTGCAATGCAATAGAAAATGGTTCACCTTCACCTTCACATTATCCACAAATTGCACCTGTTCAGAAACTTTGGTGTGTTGCAAAGCCTAGTGTTCCTGATGAAACGTTGCAAGAAGCTTTGGATTATGCTTGTGGTGAAGGTGGTGCTGATTGTTTGGAGATTACTACTCCACAAGGGAATTGTTACAATCCTGATACATTGGTTGCTCATGCTTCTTATGCATTCAATAGTTACTGGCAAAAGCATAAGAGAATTGGTGGAACTTGTGACTTTGGTGGGACTGCTATGTTGATTCATTCTGATCCAAGTTTCCTTCACTGTAGGTTTATTCTCAGCTAG
Protein sequence

>Msa1400010

MKMVWLFHLLFLCSVCIAVSGQQNIEFLNLCETSEDIATSSSHSEFPLAVSLNNEDLNKVSSNILSAETWLRLNIFPYYPSSKITNIVGKTSTFCQQDQNNLNVVLLLSSLKNLYHSLKRWGLEQDIKVSIAFDLDCFTLNSATSNHDLKMLKPMIEFLQAVNSTFSLIPSSRFSHFSDKSLNFVSSQLESMKKLGFFNHNNIINIATIIPKERKITTRKLSVTTIPIKPTPIPEIAQPPLDFPAGSPYPAPYNVPNSKPLPPLAQIVSSPPPISSPYFAPQEQPSPLPPQFVSPANSPQNGFTCPPCNAIENGSPSPSHYPQIAPVQKLWCVAKPSVPDETLQEALDYACGEGGADCLEITTPQGNCYNPDTLVAHASYAFNSYWQKHKRIGGTCDFGGTAMLIHSDPSFLHCRFILS*