Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1044330 | sp|Q9LXJ1|BIC1_ARATH | 69.355 | 62 | 18 | 1 | 58 | 119 | 64 | 124 | 1.93e-24 | 94.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1044330 | A0A072VV98 | 83.065 | 124 | 14 | 1 | 1 | 124 | 1 | 117 | 4.09e-65 | 202 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006820 | Msa1044330 | 0.800906 | 1.157061e-48 | -8.615850e-47 |
| Msa0030030 | Msa1044330 | 0.806513 | 7.930965e-50 | -8.615850e-47 |
| Msa0039200 | Msa1044330 | 0.814817 | 1.269731e-51 | -8.615850e-47 |
| Msa0047470 | Msa1044330 | 0.800668 | 1.293496e-48 | -8.615850e-47 |
| Msa0050500 | Msa1044330 | 0.800358 | 1.496127e-48 | -8.615850e-47 |
| Msa0057740 | Msa1044330 | 0.808335 | 3.258181e-50 | -8.615850e-47 |
| Msa0062750 | Msa1044330 | 0.801547 | 8.550833e-49 | -8.615850e-47 |
| Msa0077330 | Msa1044330 | 0.821313 | 4.314416e-53 | -8.615850e-47 |
| Msa0086820 | Msa1044330 | 0.811042 | 8.530433e-51 | -8.615850e-47 |
| Msa0096020 | Msa1044330 | 0.805255 | 1.457970e-49 | -8.615850e-47 |
| Msa0103060 | Msa1044330 | 0.807400 | 5.150250e-50 | -8.615850e-47 |
| Msa0604640 | Msa1044330 | 0.834247 | 3.346061e-56 | -8.615850e-47 |
| Msa0615850 | Msa1044330 | 0.802657 | 5.056820e-49 | -8.615850e-47 |
| Msa0621780 | Msa1044330 | 0.802703 | 4.945748e-49 | -8.615850e-47 |
| Msa0622390 | Msa1044330 | 0.800730 | 1.256696e-48 | -8.615850e-47 |
| Msa0638040 | Msa1044330 | 0.818818 | 1.607525e-52 | -8.615850e-47 |
| Msa0677110 | Msa1044330 | 0.809283 | 2.042704e-50 | -8.615850e-47 |
| Msa0698900 | Msa1044330 | 0.811562 | 6.580393e-51 | -8.615850e-47 |
| Msa0736930 | Msa1044330 | 0.821780 | 3.366027e-53 | -8.615850e-47 |
| Msa0739610 | Msa1044330 | 0.817469 | 3.245028e-52 | -8.615850e-47 |
| Msa0754350 | Msa1044330 | 0.820794 | 5.682270e-53 | -8.615850e-47 |
| Msa0756410 | Msa1044330 | 0.828825 | 7.248394e-55 | -8.615850e-47 |
| Msa0771020 | Msa1044330 | 0.800278 | 1.553718e-48 | -8.615850e-47 |
| Msa0771960 | Msa1044330 | 0.802003 | 6.893669e-49 | -8.615850e-47 |
| Msa0778580 | Msa1044330 | 0.823188 | 1.584454e-53 | -8.615850e-47 |
| Msa0781900 | Msa1044330 | 0.808991 | 2.359484e-50 | -8.615850e-47 |
| Msa0787720 | Msa1044330 | 0.815332 | 9.762294e-52 | -8.615850e-47 |
| Msa0793060 | Msa1044330 | 0.801733 | 7.833739e-49 | -8.615850e-47 |
| Msa0795710 | Msa1044330 | 0.803796 | 2.938360e-49 | -8.615850e-47 |
| Msa0796610 | Msa1044330 | 0.808308 | 3.300864e-50 | -8.615850e-47 |
| Msa0797620 | Msa1044330 | 0.806757 | 7.045533e-50 | -8.615850e-47 |
| Msa0797810 | Msa1044330 | 0.806287 | 8.852625e-50 | -8.615850e-47 |
| Msa0815950 | Msa1044330 | 0.840232 | 9.841914e-58 | -8.615850e-47 |
| Msa0833450 | Msa1044330 | 0.810240 | 1.271933e-50 | -8.615850e-47 |
| Msa0833910 | Msa1044330 | 0.812486 | 4.138251e-51 | -8.615850e-47 |
| Msa0834160 | Msa1044330 | 0.832275 | 1.037589e-55 | -8.615850e-47 |
| Msa0133670 | Msa1044330 | 0.813307 | 2.734473e-51 | -8.615850e-47 |
| Msa0133880 | Msa1044330 | 0.813400 | 2.609563e-51 | -8.615850e-47 |
| Msa0153640 | Msa1044330 | 0.817362 | 3.429295e-52 | -8.615850e-47 |
| Msa0169960 | Msa1044330 | 0.838430 | 2.889365e-57 | -8.615850e-47 |
| Msa0175720 | Msa1044330 | 0.812890 | 3.375507e-51 | -8.615850e-47 |
| Msa0184120 | Msa1044330 | 0.817436 | 3.300585e-52 | -8.615850e-47 |
| Msa0197730 | Msa1044330 | 0.800069 | 1.713094e-48 | -8.615850e-47 |
| Msa0203180 | Msa1044330 | 0.800033 | 1.742045e-48 | -8.615850e-47 |
| Msa0216010 | Msa1044330 | 0.804085 | 2.558919e-49 | -8.615850e-47 |
| Msa0216560 | Msa1044330 | 0.814538 | 1.464399e-51 | -8.615850e-47 |
| Msa0222720 | Msa1044330 | 0.811176 | 7.981139e-51 | -8.615850e-47 |
| Msa0369750 | Msa1044330 | 0.818367 | 2.033654e-52 | -8.615850e-47 |
| Msa0423110 | Msa1044330 | 0.826355 | 2.839419e-54 | -8.615850e-47 |
| Msa0423600 | Msa1044330 | 0.800440 | 1.439610e-48 | -8.615850e-47 |
| Msa0427050 | Msa1044330 | 0.833792 | 4.349839e-56 | -8.615850e-47 |
| Msa0431940 | Msa1044330 | 0.806142 | 9.497729e-50 | -8.615850e-47 |
| Msa0448400 | Msa1044330 | 0.809431 | 1.899040e-50 | -8.615850e-47 |
| Msa0456480 | Msa1044330 | 0.841634 | 4.218803e-58 | -8.615850e-47 |
| Msa0473320 | Msa1044330 | 0.802714 | 4.921985e-49 | -8.615850e-47 |
| Msa0473450 | Msa1044330 | 0.816303 | 5.927642e-52 | -8.615850e-47 |
| Msa0982590 | Msa1044330 | 0.803202 | 3.902146e-49 | -8.615850e-47 |
| Msa0984840 | Msa1044330 | 0.826304 | 2.919748e-54 | -8.615850e-47 |
| Msa0985430 | Msa1044330 | 0.826977 | 2.017439e-54 | -8.615850e-47 |
| Msa0986620 | Msa1044330 | 0.813494 | 2.488123e-51 | -8.615850e-47 |
| Msa1016110 | Msa1044330 | 0.817581 | 3.061548e-52 | -8.615850e-47 |
| Msa1031220 | Msa1044330 | 0.800020 | 1.752684e-48 | -8.615850e-47 |
| Msa1044330 | Msa1102200 | 0.816126 | 6.492924e-52 | -8.615850e-47 |
| Msa1044330 | Msa1117830 | 0.806610 | 7.565725e-50 | -8.615850e-47 |
| Msa1044330 | Msa1138600 | 0.804921 | 1.712926e-49 | -8.615850e-47 |
| Msa1044330 | Msa1161160 | 0.813540 | 2.430978e-51 | -8.615850e-47 |
| Msa1044330 | Msa1173410 | 0.806435 | 8.238218e-50 | -8.615850e-47 |
| Msa1044330 | Msa1195860 | 0.809367 | 1.960048e-50 | -8.615850e-47 |
| Msa1044330 | Msa1209790 | 0.806786 | 6.947116e-50 | -8.615850e-47 |
| Msa1044330 | Msa1228330 | 0.803272 | 3.772779e-49 | -8.615850e-47 |
| Msa1044330 | Msa1231520 | 0.830670 | 2.576850e-55 | -8.615850e-47 |
| Msa1044330 | Msa1235770 | 0.807500 | 4.904829e-50 | -8.615850e-47 |
| Msa1044330 | Msa1242990 | 0.857868 | 1.216987e-62 | -8.615850e-47 |
| Msa1044330 | Msa1248400 | 0.800346 | 1.504964e-48 | -8.615850e-47 |
| Msa1044330 | Msa1272130 | 0.801522 | 8.655683e-49 | -8.615850e-47 |
| Msa1044330 | Msa1274310 | 0.805631 | 1.215839e-49 | -8.615850e-47 |
| Msa1044330 | Msa1282210 | 0.800345 | 1.505343e-48 | -8.615850e-47 |
| Msa1044330 | Msa1284390 | 0.862010 | 6.859416e-64 | -8.615850e-47 |
| Msa1044330 | Msa1287640 | 0.829515 | 4.930785e-55 | -8.615850e-47 |
| Msa1044330 | Msa1289340 | 0.829173 | 5.970443e-55 | -8.615850e-47 |
| Msa1044330 | Msa1306700 | 0.818453 | 1.944512e-52 | -8.615850e-47 |
| Msa1044330 | Msa1323920 | 0.842839 | 2.023832e-58 | -8.615850e-47 |
| Msa1044330 | Msa1327820 | 0.801781 | 7.656817e-49 | -8.615850e-47 |
| Msa1044330 | Msa1333430 | 0.815825 | 7.581227e-52 | -8.615850e-47 |
| Msa1044330 | Msa1342400 | 0.832620 | 8.519259e-56 | -8.615850e-47 |
| Msa1044330 | Msa1346540 | 0.829729 | 4.374755e-55 | -8.615850e-47 |
| Msa1044330 | Msa1379370 | 0.801361 | 9.338137e-49 | -8.615850e-47 |
| Msa1044330 | Msa1384980 | 0.821177 | 4.637107e-53 | -8.615850e-47 |
| Msa1044330 | Msa1385940 | 0.808284 | 3.340065e-50 | -8.615850e-47 |
| Msa1044330 | Msa1388070 | 0.813421 | 2.582268e-51 | -8.615850e-47 |
| Msa1044330 | Msa1397540 | 0.818574 | 1.825922e-52 | -8.615850e-47 |
| Msa1044330 | Msa1403950 | 0.811286 | 7.552598e-51 | -8.615850e-47 |
| Msa1044330 | Msa1405470 | 0.849043 | 4.163798e-60 | -8.615850e-47 |
| Msa1044330 | Msa1413220 | 0.805206 | 1.492774e-49 | -8.615850e-47 |
| Msa1044330 | Msa1440180 | 0.800043 | 1.734472e-48 | -8.615850e-47 |
| Msa1044330 | Msa1452200 | 0.862098 | 6.445454e-64 | -8.615850e-47 |
| Msa0256170 | Msa1044330 | 0.854495 | 1.185221e-61 | -8.615850e-47 |
| Msa0256430 | Msa1044330 | 0.831681 | 1.453947e-55 | -8.615850e-47 |
| Msa0264020 | Msa1044330 | 0.802293 | 6.008963e-49 | -8.615850e-47 |
| Msa0284870 | Msa1044330 | 0.800418 | 1.454984e-48 | -8.615850e-47 |
| Msa0295840 | Msa1044330 | 0.845625 | 3.613795e-59 | -8.615850e-47 |
| Msa0311330 | Msa1044330 | 0.804447 | 2.151220e-49 | -8.615850e-47 |
| Msa0330270 | Msa1044330 | 0.809680 | 1.678614e-50 | -8.615850e-47 |
| Msa0334180 | Msa1044330 | 0.812684 | 3.745110e-51 | -8.615850e-47 |
| Msa0336270 | Msa1044330 | 0.801375 | 9.275636e-49 | -8.615850e-47 |
| Msa0339340 | Msa1044330 | 0.805768 | 1.138157e-49 | -8.615850e-47 |
| Msa0496460 | Msa1044330 | 0.802698 | 4.957590e-49 | -8.615850e-47 |
| Msa0520810 | Msa1044330 | 0.800357 | 1.496707e-48 | -8.615850e-47 |
| Msa0549330 | Msa1044330 | 0.856092 | 4.063299e-62 | -8.615850e-47 |
| Msa0580540 | Msa1044330 | 0.808961 | 2.393892e-50 | -8.615850e-47 |
| Msa0598250 | Msa1044330 | 0.804774 | 1.838414e-49 | -8.615850e-47 |
| Msa0844350 | Msa1044330 | 0.815097 | 1.100473e-51 | -8.615850e-47 |
| Msa0861560 | Msa1044330 | 0.815793 | 7.707614e-52 | -8.615850e-47 |
| Msa0887070 | Msa1044330 | 0.820128 | 8.080173e-53 | -8.615850e-47 |
| Msa0913120 | Msa1044330 | 0.855960 | 4.441801e-62 | -8.615850e-47 |
| Msa0950410 | Msa1044330 | 0.826209 | 3.075565e-54 | -8.615850e-47 |
| Msa0978000 | Msa1044330 | 0.811078 | 8.380374e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1044330 | MtrunA17_Chr1g0178641 | 83.065 | 124 | 14 | 1 | 1 | 124 | 20 | 136 | 1.04e-68 | 202 |
| Msa1044330 | MtrunA17_Chr7g0266241 | 41.358 | 162 | 51 | 5 | 1 | 119 | 10 | 170 | 1.77e-29 | 105 |
| Msa1044330 | MtrunA17_Chr2g0321471 | 53.226 | 62 | 28 | 1 | 56 | 116 | 71 | 132 | 2.06e-15 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1044330 | AT3G52740.1 | 69.355 | 62 | 18 | 1 | 58 | 119 | 64 | 124 | 1.96e-25 | 94.0 |
| Msa1044330 | AT3G44450.1 | 41.379 | 87 | 43 | 2 | 31 | 117 | 18 | 96 | 9.45e-17 | 70.9 |
Find 23 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCAATTCAATATCAATT+TGG | 0.251682 | 7_2:+2694867 | None:intergenic |
| CTTTCTTCTTCCTCAACAAT+AGG | 0.318419 | 7_2:+2694746 | None:intergenic |
| TCTTCTTGTCCCCACATATC+TGG | 0.329231 | 7_2:+2694668 | None:intergenic |
| ACTCATAGATAACAATATTA+AGG | 0.333312 | 7_2:-2694834 | Msa1044330:CDS |
| CTTTGGTTCAAGAAGCTACT+AGG | 0.363188 | 7_2:-2694565 | Msa1044330:CDS |
| TTGAAATGGCTGGAAGAGTA+TGG | 0.372715 | 7_2:-2694694 | Msa1044330:CDS |
| AATAAACTCAACACCTATAG+TGG | 0.387379 | 7_2:+2694533 | None:intergenic |
| TGTGACTGCTAGAACAGCTT+TGG | 0.411343 | 7_2:-2694582 | Msa1044330:CDS |
| TAGGATTCAGATTCCACTAT+AGG | 0.435137 | 7_2:-2694546 | None:intergenic |
| GAAGAGACATAGAGTTGAAA+TGG | 0.437286 | 7_2:-2694708 | Msa1044330:CDS |
| AAGAAGAGTACTTGAAGGAT+TGG | 0.465508 | 7_2:-2694652 | Msa1044330:CDS |
| CATGAGATTTAGTAGATGAT+TGG | 0.476408 | 7_2:+2694891 | None:intergenic |
| AGACATAGAGTTGAAATGGC+TGG | 0.496362 | 7_2:-2694704 | Msa1044330:CDS |
| GAGTATGGATTCCAGATATG+TGG | 0.497888 | 7_2:-2694679 | Msa1044330:CDS |
| AGTATGGATTCCAGATATGT+GGG | 0.543345 | 7_2:-2694678 | Msa1044330:CDS |
| TGAAATTGCATATATGAAGA+AGG | 0.550557 | 7_2:-2694777 | Msa1044330:CDS |
| TTGTTATCTATGAGTTGATG+AGG | 0.558529 | 7_2:+2694842 | None:intergenic |
| AAGAAGAAAGTGGAAGAGAG+AGG | 0.561315 | 7_2:-2694733 | Msa1044330:CDS |
| GGGACAAGAAGAGTACTTGA+AGG | 0.578419 | 7_2:-2694657 | Msa1044330:CDS |
| AATTGCATATATGAAGAAGG+TGG | 0.595793 | 7_2:-2694774 | Msa1044330:CDS |
| ATTGTTGAGGAAGAAGAAAG+TGG | 0.598074 | 7_2:-2694743 | Msa1044330:CDS |
| TTGCTAGCAGACATCAAAGG+AGG | 0.645009 | 7_2:+2694608 | None:intergenic |
| GTATGGATTCCAGATATGTG+GGG | 0.706622 | 7_2:-2694677 | Msa1044330:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTCAATTCAATATCAATT+TGG | + | chr7_2:2694582-2694601 | None:intergenic | 20.0% |
| !! | ACTCATAGATAACAATATTA+AGG | - | chr7_2:2694612-2694631 | Msa1044330:CDS | 20.0% |
| ! | TGAAATTGCATATATGAAGA+AGG | - | chr7_2:2694669-2694688 | Msa1044330:CDS | 25.0% |
| CATGAGATTTAGTAGATGAT+TGG | + | chr7_2:2694558-2694577 | None:intergenic | 30.0% | |
| !! | TTGTTATCTATGAGTTGATG+AGG | + | chr7_2:2694607-2694626 | None:intergenic | 30.0% |
| AATTGCATATATGAAGAAGG+TGG | - | chr7_2:2694672-2694691 | Msa1044330:CDS | 30.0% | |
| CTTTCTTCTTCCTCAACAAT+AGG | + | chr7_2:2694703-2694722 | None:intergenic | 35.0% | |
| ATTGTTGAGGAAGAAGAAAG+TGG | - | chr7_2:2694703-2694722 | Msa1044330:CDS | 35.0% | |
| GAAGAGACATAGAGTTGAAA+TGG | - | chr7_2:2694738-2694757 | Msa1044330:CDS | 35.0% | |
| AGTATGGATTCCAGATATGT+GGG | - | chr7_2:2694768-2694787 | Msa1044330:CDS | 35.0% | |
| !! | AAGAAGAGTACTTGAAGGAT+TGG | - | chr7_2:2694794-2694813 | Msa1044330:CDS | 35.0% |
| ! | ATTTTGCTAGCAGACATCAA+AGG | + | chr7_2:2694844-2694863 | None:intergenic | 35.0% |
| AAGAAGAAAGTGGAAGAGAG+AGG | - | chr7_2:2694713-2694732 | Msa1044330:CDS | 40.0% | |
| AGACATAGAGTTGAAATGGC+TGG | - | chr7_2:2694742-2694761 | Msa1044330:CDS | 40.0% | |
| TTGAAATGGCTGGAAGAGTA+TGG | - | chr7_2:2694752-2694771 | Msa1044330:CDS | 40.0% | |
| GAGTATGGATTCCAGATATG+TGG | - | chr7_2:2694767-2694786 | Msa1044330:CDS | 40.0% | |
| GTATGGATTCCAGATATGTG+GGG | - | chr7_2:2694769-2694788 | Msa1044330:CDS | 40.0% | |
| ! | CTTTGGTTCAAGAAGCTACT+AGG | - | chr7_2:2694881-2694900 | Msa1044330:CDS | 40.0% |
| !! | GGTGGTTTTGCCTATTGTTG+AGG | - | chr7_2:2694690-2694709 | Msa1044330:CDS | 45.0% |
| TCTTCTTGTCCCCACATATC+TGG | + | chr7_2:2694781-2694800 | None:intergenic | 45.0% | |
| !! | GGGACAAGAAGAGTACTTGA+AGG | - | chr7_2:2694789-2694808 | Msa1044330:CDS | 45.0% |
| TTGCTAGCAGACATCAAAGG+AGG | + | chr7_2:2694841-2694860 | None:intergenic | 45.0% | |
| TGTGACTGCTAGAACAGCTT+TGG | - | chr7_2:2694864-2694883 | Msa1044330:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 2694547 | 2694921 | 2694547 | ID=Msa1044330;Name=Msa1044330 |
| chr7_2 | mRNA | 2694547 | 2694921 | 2694547 | ID=Msa1044330-mRNA-1;Parent=Msa1044330;Name=Msa1044330-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|124 |
| chr7_2 | exon | 2694547 | 2694921 | 2694547 | ID=Msa1044330-mRNA-1:exon:701;Parent=Msa1044330-mRNA-1 |
| chr7_2 | CDS | 2694547 | 2694921 | 2694547 | ID=Msa1044330-mRNA-1:cds;Parent=Msa1044330-mRNA-1 |
| Gene Sequence |
| Protein sequence |
