AlfalfaGEDB | Msa1258060

M. sativa cultivar zhongmu-4 / Msa1258060

KEGG: K14423
GO: GO:0000254 GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006629 GO:0006694 GO:0008150 GO:0008152 GO:0008202 GO:0008610 GO:0009058 GO:0009987 GO:0012505 GO:0016020 GO:0016125 GO:0016126 GO:0016491 GO:0016705 GO:0016709 GO:0030258 GO:0031984 GO:0034440 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0080064 GO:0098827 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617
PFAM: PF04116.16

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258060	XP_003608797.1	98.680	303	4	0	1	303	1	303	0.0	624

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258060	sp\|Q8L7W5\|SMO11_ARATH	76.109	293	68	1	1	291	1	293	1.07e-174	488

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258060	G7JK82	98.680	303	4	0	1	303	1	303	0.0	624

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0009290	Msa1258060	0.800120	1.672499e-48	-8.615850e-47
Msa0014970	Msa1258060	-0.816821	4.538967e-52	-8.615850e-47
Msa0027030	Msa1258060	-0.800573	1.353052e-48	-8.615850e-47
Msa0029730	Msa1258060	0.837936	3.873641e-57	-8.615850e-47
Msa0034050	Msa1258060	-0.813768	2.165634e-51	-8.615850e-47
Msa0050320	Msa1258060	-0.842174	3.038039e-58	-8.615850e-47
Msa0053260	Msa1258060	-0.807435	5.061198e-50	-8.615850e-47
Msa0081700	Msa1258060	-0.803016	4.262293e-49	-8.615850e-47
Msa0094900	Msa1258060	-0.821496	3.914205e-53	-8.615850e-47
Msa0098750	Msa1258060	-0.812986	3.216909e-51	-8.615850e-47
Msa0099090	Msa1258060	-0.816697	4.837475e-52	-8.615850e-47
Msa0108500	Msa1258060	-0.805168	1.520880e-49	-8.615850e-47
Msa0608940	Msa1258060	-0.840955	6.367348e-58	-8.615850e-47
Msa0612880	Msa1258060	-0.803515	3.360012e-49	-8.615850e-47
Msa0617470	Msa1258060	-0.808731	2.681116e-50	-8.615850e-47
Msa0649530	Msa1258060	-0.849150	3.889803e-60	-8.615850e-47
Msa0659020	Msa1258060	-0.806015	1.010046e-49	-8.615850e-47
Msa0691740	Msa1258060	-0.857127	2.016540e-62	-8.615850e-47
Msa0700250	Msa1258060	-0.806777	6.977495e-50	-8.615850e-47
Msa0702020	Msa1258060	-0.801206	1.004434e-48	-8.615850e-47
Msa0704690	Msa1258060	0.802243	6.154775e-49	-8.615850e-47
Msa0733240	Msa1258060	-0.818961	1.491650e-52	-8.615850e-47
Msa0742690	Msa1258060	-0.802207	6.258492e-49	-8.615850e-47
Msa0779830	Msa1258060	-0.815055	1.124573e-51	-8.615850e-47
Msa0812020	Msa1258060	-0.805272	1.446488e-49	-8.615850e-47
Msa0827560	Msa1258060	-0.801132	1.040212e-48	-8.615850e-47
Msa0831460	Msa1258060	-0.802094	6.602375e-49	-8.615850e-47
Msa0127910	Msa1258060	-0.810555	1.087497e-50	-8.615850e-47
Msa0138340	Msa1258060	-0.814255	1.690742e-51	-8.615850e-47
Msa0147960	Msa1258060	-0.813935	1.989178e-51	-8.615850e-47
Msa0154720	Msa1258060	-0.842814	2.054305e-58	-8.615850e-47
Msa0169710	Msa1258060	0.812700	3.715838e-51	-8.615850e-47
Msa0174060	Msa1258060	-0.810797	9.641536e-51	-8.615850e-47
Msa0178440	Msa1258060	-0.808332	3.262472e-50	-8.615850e-47
Msa0186400	Msa1258060	-0.802079	6.650334e-49	-8.615850e-47
Msa0194750	Msa1258060	-0.820990	5.122603e-53	-8.615850e-47
Msa0195030	Msa1258060	0.816699	4.834330e-52	-8.615850e-47
Msa0208040	Msa1258060	-0.804898	1.731830e-49	-8.615850e-47
Msa0208710	Msa1258060	-0.816934	4.280508e-52	-8.615850e-47
Msa0209370	Msa1258060	-0.820027	8.520507e-53	-8.615850e-47
Msa0225070	Msa1258060	-0.813689	2.254532e-51	-8.615850e-47
Msa0227970	Msa1258060	-0.801832	7.475804e-49	-8.615850e-47
Msa0236690	Msa1258060	-0.813215	2.865210e-51	-8.615850e-47
Msa0237180	Msa1258060	0.821495	3.917752e-53	-8.615850e-47
Msa1228600	Msa1258060	-0.804197	2.424543e-49	-8.615850e-47
Msa1230190	Msa1258060	-0.807457	5.008545e-50	-8.615850e-47
Msa1240240	Msa1258060	-0.800091	1.695336e-48	-8.615850e-47
Msa1251500	Msa1258060	0.851303	9.687988e-61	-8.615850e-47
Msa1258060	Msa1260520	-0.810244	1.269335e-50	-8.615850e-47
Msa1258060	Msa1266120	-0.816347	5.794725e-52	-8.615850e-47
Msa1258060	Msa1272770	-0.828220	1.014635e-54	-8.615850e-47
Msa1258060	Msa1286950	-0.807117	5.912582e-50	-8.615850e-47
Msa1258060	Msa1292140	0.834774	2.467840e-56	-8.615850e-47
Msa1258060	Msa1297220	-0.806335	8.649384e-50	-8.615850e-47
Msa1258060	Msa1299220	0.802398	5.718914e-49	-8.615850e-47
Msa1258060	Msa1320660	-0.842263	2.877084e-58	-8.615850e-47
Msa1258060	Msa1323260	-0.804615	1.983759e-49	-8.615850e-47
Msa1258060	Msa1342360	-0.801423	9.069089e-49	-8.615850e-47
Msa1258060	Msa1343450	-0.813869	2.057724e-51	-8.615850e-47
Msa1258060	Msa1346340	-0.818182	2.239697e-52	-8.615850e-47
Msa1258060	Msa1348860	-0.801786	7.639764e-49	-8.615850e-47
Msa1258060	Msa1363270	-0.803926	2.760255e-49	-8.615850e-47
Msa1258060	Msa1363280	-0.827102	1.883273e-54	-8.615850e-47
Msa1258060	Msa1371540	-0.807623	4.617716e-50	-8.615850e-47
Msa1258060	Msa1382100	-0.803633	3.176028e-49	-8.615850e-47
Msa1258060	Msa1392570	-0.837210	5.945048e-57	-8.615850e-47
Msa1258060	Msa1400240	-0.809482	1.851509e-50	-8.615850e-47
Msa1258060	Msa1408990	0.988455	4.390991e-174	-8.615850e-47
Msa1258060	Msa1432250	-0.809098	2.238196e-50	-8.615850e-47
Msa1258060	Msa1450850	-0.819092	1.392764e-52	-8.615850e-47
Msa0370350	Msa1258060	-0.806105	9.666313e-50	-8.615850e-47
Msa0372100	Msa1258060	0.800853	1.185776e-48	-8.615850e-47
Msa0390590	Msa1258060	-0.810848	9.396630e-51	-8.615850e-47
Msa0456450	Msa1258060	-0.800769	1.233893e-48	-8.615850e-47
Msa0480940	Msa1258060	0.956718	1.522603e-114	-8.615850e-47
Msa1031530	Msa1258060	-0.813030	3.146049e-51	-8.615850e-47
Msa1078590	Msa1258060	-0.808733	2.679455e-50	-8.615850e-47
Msa1078760	Msa1258060	-0.803997	2.668631e-49	-8.615850e-47
Msa1093190	Msa1258060	-0.816824	4.530439e-52	-8.615850e-47
Msa0266140	Msa1258060	-0.804715	1.891135e-49	-8.615850e-47
Msa0267450	Msa1258060	-0.820254	7.558480e-53	-8.615850e-47
Msa0267510	Msa1258060	-0.811996	5.294194e-51	-8.615850e-47
Msa0269660	Msa1258060	-0.804460	2.136966e-49	-8.615850e-47
Msa0304060	Msa1258060	-0.802988	4.319400e-49	-8.615850e-47
Msa0304350	Msa1258060	-0.826930	2.069755e-54	-8.615850e-47
Msa0305910	Msa1258060	-0.826889	2.117637e-54	-8.615850e-47
Msa0307430	Msa1258060	0.804921	1.712278e-49	-8.615850e-47
Msa0315660	Msa1258060	0.824142	9.473795e-54	-8.615850e-47
Msa0340830	Msa1258060	-0.828612	8.161753e-55	-8.615850e-47
Msa0340870	Msa1258060	-0.826129	3.213720e-54	-8.615850e-47
Msa1136770	Msa1258060	-0.802241	6.160849e-49	-8.615850e-47
Msa1169590	Msa1258060	-0.802167	6.379141e-49	-8.615850e-47
Msa1197820	Msa1258060	0.817613	3.011162e-52	-8.615850e-47
Msa1207880	Msa1258060	0.956488	2.619447e-114	-8.615850e-47
Msa0483090	Msa1258060	-0.839153	1.878125e-57	-8.615850e-47
Msa0526490	Msa1258060	-0.815356	9.640789e-52	-8.615850e-47
Msa0526500	Msa1258060	-0.824161	9.377508e-54	-8.615850e-47
Msa0527210	Msa1258060	-0.807075	6.033584e-50	-8.615850e-47
Msa0538050	Msa1258060	-0.802283	6.038319e-49	-8.615850e-47
Msa0555510	Msa1258060	-0.829660	4.546745e-55	-8.615850e-47
Msa0849290	Msa1258060	-0.805550	1.264867e-49	-8.615850e-47
Msa0852090	Msa1258060	-0.816880	4.402026e-52	-8.615850e-47
Msa0907170	Msa1258060	0.803915	2.776007e-49	-8.615850e-47
Msa0917630	Msa1258060	-0.815630	8.377432e-52	-8.615850e-47
Msa0967900	Msa1258060	-0.819853	9.339685e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258060	MtrunA17_Chr4g0056801	98.680	303	4	0	1	303	1	303	0.0	624
Msa1258060	MtrunA17_Chr7g0245771	68.786	173	54	0	112	284	24	196	7.77e-93	274
Msa1258060	MtrunA17_Chr8g0364601	41.887	265	150	3	22	285	6	267	4.20e-67	211
Msa1258060	MtrunA17_Chr5g0414141	43.651	252	138	3	33	283	17	265	1.27e-66	209
Msa1258060	MtrunA17_Chr6g0466381	43.333	240	132	3	22	260	6	242	1.10e-62	198
Msa1258060	MtrunA17_Chr6g0465561	43.333	240	132	3	22	260	6	242	1.25e-62	198
Msa1258060	MtrunA17_Chr7g0245781	53.982	113	52	0	1	113	1	113	7.79e-39	133
Msa1258060	MtrunA17_Chr5g0416951	31.788	151	94	4	129	274	101	247	1.11e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258060	AT4G12110.1	76.109	293	68	1	1	291	1	293	1.08e-175	488
Msa1258060	AT4G22756.1	74.247	299	73	2	1	295	1	299	1.15e-171	477
Msa1258060	AT4G22755.1	69.072	291	87	2	1	290	1	289	2.68e-156	438
Msa1258060	AT1G07420.1	42.642	265	144	4	19	282	3	260	6.54e-69	215
Msa1258060	AT2G29390.3	43.033	244	135	3	33	275	10	250	7.55e-66	207
Msa1258060	AT2G29390.2	41.473	258	147	3	19	275	3	257	9.37e-66	207
Msa1258060	AT1G07420.2	46.970	198	101	1	85	282	29	222	1.44e-64	203
Msa1258060	AT2G29390.5	45.550	191	104	0	85	275	26	216	9.98e-63	198
Msa1258060	AT2G29390.1	39.630	270	147	4	19	275	3	269	2.61e-62	199
Msa1258060	AT2G29390.6	47.222	180	91	1	102	281	1	176	7.46e-61	192
Msa1258060	AT2G29390.4	47.222	180	91	1	102	281	1	176	7.46e-61	192
Msa1258060	AT1G14290.1	29.224	219	142	6	64	274	30	243	7.46e-14	70.5
Msa1258060	AT1G14290.2	33.113	151	92	4	129	274	23	169	1.24e-12	65.9

Find 106 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGGCGGAGATCTTGATTT+TGG	0.150585	8_2:+59162039	Msa1258060:CDS
ACATATTGTGATTACATCTA+TGG	0.218915	8_2:+59164076	Msa1258060:CDS
ATAGATTTCTGCATAATAAA+TGG	0.221052	8_2:+59161945	Msa1258060:CDS
TATATGTCTACAAGAAATTT+TGG	0.231686	8_2:-59164552	None:intergenic
CTTGGACCGGCTATGGTTCC+TGG	0.248242	8_2:+59162076	Msa1258060:CDS
TATTGTAGATGATTGGAATT+AGG	0.278974	8_2:+59161834	Msa1258060:intron
ATTTATTATGCAGAAATCTA+TGG	0.288607	8_2:-59161943	None:intergenic
TATAGCAATTAAGCATTTCA+TGG	0.305557	8_2:-59161675	None:intergenic
CTGGTCACATGATTACATTT+TGG	0.315803	8_2:+59162095	Msa1258060:CDS
AAAATGTTCTTCCTTGTTGT+TGG	0.321889	8_2:+59161709	Msa1258060:CDS
TCCCTGTGGATTGTAAATTC+TGG	0.327737	8_2:+59164752	Msa1258060:three_prime_UTR
TGGAGGATTATACGAATTAC+TGG	0.338989	8_2:+59161918	Msa1258060:CDS
TGCTCAGCGCCACCATAAAA+TGG	0.348188	8_2:-59163995	None:intergenic
TGTGATTGTGATTACCTTGA+TGG	0.355111	8_2:-59161758	None:intergenic
TAGATTTCTGCATAATAAAT+GGG	0.360869	8_2:+59161946	Msa1258060:CDS
ACTAGTAAAATAGATGTCTT+TGG	0.366009	8_2:+59164581	Msa1258060:three_prime_UTR
GAAGAGAAAGTAATCGGATT+TGG	0.370486	8_2:-59161527	None:intergenic
AGATGTCTTTGGTTAGCTTC+AGG	0.374928	8_2:+59164592	Msa1258060:three_prime_UTR
CGCCGGCAATCTCTCTCTCT+AGG	0.380944	8_2:+59161387	Msa1258060:five_prime_UTR
ATGGAGAGATAAACGAACTT+TGG	0.389544	8_2:-59161656	None:intergenic
CCGGCGGCGGGAGAGGGGAG+AGG	0.399811	8_2:-59161370	None:intergenic
TGCGGCGCCTTATGCACATT+GGG	0.401426	8_2:+59162021	Msa1258060:CDS
CGAGGGAGAAGATGAGGAAG+AGG	0.414192	8_2:-59161567	None:intergenic
TGGAGAGATAAACGAACTTT+GGG	0.417171	8_2:-59161655	None:intergenic
GAGATTGCCGGCGGCGGGAG+AGG	0.427256	8_2:-59161377	None:intergenic
TCTCGGCGTCGTGGAGAGAT+TGG	0.432872	8_2:-59161447	None:intergenic
AGGATGAAGACTAGTAGAAC+GGG	0.441695	8_2:+59164612	Msa1258060:three_prime_UTR
CATGAATATCAAGCTCCAAT+TGG	0.447424	8_2:+59161995	Msa1258060:CDS
TGGAAGATCATATAATGAAC+AGG	0.447595	8_2:+59164459	Msa1258060:CDS
GTGAAACAGACTTCCTCCTC+TGG	0.450453	8_2:+59164779	Msa1258060:three_prime_UTR
ATCTCCGCCCAATGTGCATA+AGG	0.458453	8_2:-59162028	None:intergenic
TAAGGCGCCGCAAATCCAAT+TGG	0.460635	8_2:-59162010	None:intergenic
AGATTGCCGGCGGCGGGAGA+GGG	0.462188	8_2:-59161376	None:intergenic
ATGACAGAAGAGAAAGTAAT+CGG	0.467469	8_2:-59161533	None:intergenic
AGAGAGAGATTGCCGGCGGC+GGG	0.468525	8_2:-59161382	None:intergenic
CACGATTACCATCATTATGT+TGG	0.470556	8_2:+59164019	Msa1258060:CDS
TTGCGGCGCCTTATGCACAT+TGG	0.470678	8_2:+59162020	Msa1258060:CDS
AGGTGGTGTATGTTGTATGT+TGG	0.473393	8_2:+59164516	Msa1258060:three_prime_UTR
GTAGTTGAACCATAGGGTTT+CGG	0.479091	8_2:-59161497	None:intergenic
TCAAGCTCCAATTGGATTTG+CGG	0.480625	8_2:+59162003	Msa1258060:CDS
CGTTTCTTATCCTTCCATCA+AGG	0.481297	8_2:+59161744	Msa1258060:CDS
CTGAAGGAAGATTCGACAAA+TGG	0.482061	8_2:+59164424	Msa1258060:CDS
TTCCAGAATTTACAATCCAC+AGG	0.484330	8_2:-59164754	None:intergenic
TGGTGGATGATGCGCTGGAC+TGG	0.495240	8_2:-59161315	None:intergenic
GAACCATAGGGTTTCGGCGA+AGG	0.497179	8_2:-59161491	None:intergenic
GAGGAACGAGGGAGAAGATG+AGG	0.498130	8_2:-59161573	None:intergenic
TCACCTTCGCCGAAACCCTA+TGG	0.501858	8_2:+59161488	Msa1258060:CDS
TCAGAAGAAGATACTTCAAA+AGG	0.505907	8_2:+59164224	Msa1258060:CDS
ACCCTAGAGAGAGAGATTGC+CGG	0.512601	8_2:-59161389	None:intergenic
GAGAGAGAGATTGCCGGCGG+CGG	0.513252	8_2:-59161383	None:intergenic
ATGTCAGGTATGACTTCCCC+TGG	0.513299	8_2:+59163954	Msa1258060:intron
TAGATGATTGGAATTAGGAC+GGG	0.513499	8_2:+59161839	Msa1258060:intron
CCTCTCCCCTCTCCCGCCGC+CGG	0.514345	8_2:+59161370	Msa1258060:five_prime_UTR
ACAAATGGTGCAGCGCGGAA+TGG	0.520094	8_2:+59164439	Msa1258060:CDS
TAGCGGAGTAGTTGAACCAT+AGG	0.521461	8_2:-59161504	None:intergenic
TAATGTAGAGAGAGTGTGTA+TGG	0.524622	8_2:-59161335	None:intergenic
CGGCGGCGGGAGAGGGGAGA+GGG	0.531448	8_2:-59161369	None:intergenic
GATTGCCGGCGGCGGGAGAG+GGG	0.531911	8_2:-59161375	None:intergenic
AAACTGATGCAAAATTGCTC+TGG	0.537490	8_2:-59164051	None:intergenic
GTAGATGATTGGAATTAGGA+CGG	0.538786	8_2:+59161838	Msa1258060:intron
GATTACCATCATTATGTTGG+AGG	0.541524	8_2:+59164022	Msa1258060:CDS
GCGTCGTGGAGAGATTGGTA+AGG	0.542904	8_2:-59161442	None:intergenic
ATTGCGACCGAGAGAGATCT+CGG	0.545066	8_2:-59161464	None:intergenic
CACGACGCCGAGATCTCTCT+CGG	0.545396	8_2:+59161457	Msa1258060:CDS
ATGTGACCAGGAACCATAGC+CGG	0.548009	8_2:-59162082	None:intergenic
GAGATAAACGAACTTTGGGC+TGG	0.549681	8_2:-59161651	None:intergenic
GCCGGCAATCTCTCTCTCTA+GGG	0.550744	8_2:+59161388	Msa1258060:five_prime_UTR
ACGGGGTTACCGTTACCGTC+TGG	0.551559	8_2:+59161857	Msa1258060:CDS
GGATGATGCGCTGGACTGGA+TGG	0.552322	8_2:-59161311	None:intergenic
GTGTATGGTGGATGATGCGC+TGG	0.556925	8_2:-59161320	None:intergenic
TTTGTCCTCCAACATAATGA+TGG	0.557552	8_2:-59164027	None:intergenic
GTACCCGCTGTGTGTATCAA+TGG	0.560522	8_2:-59162146	None:intergenic
AAAGTAATCGGATTTGGTAG+CGG	0.569740	8_2:-59161521	None:intergenic
TGGATCTTGTACTCATCGAA+AGG	0.573332	8_2:-59161631	None:intergenic
AAACGAGTGGAAGAGGAACG+AGG	0.585179	8_2:-59161585	None:intergenic
GGCGCCTTATGCACATTGGG+CGG	0.588545	8_2:+59162024	Msa1258060:CDS
GATGCGCTGGACTGGATGGA+TGG	0.590898	8_2:-59161307	None:intergenic
AGCGGAGTAGTTGAACCATA+GGG	0.591782	8_2:-59161503	None:intergenic
GGATAAGAAACGAGTTGAAG+AGG	0.594918	8_2:-59161733	None:intergenic
GGATCTTGTACTCATCGAAA+GGG	0.594949	8_2:-59161630	None:intergenic
ATCTCAAATCCAGACGGTAA+CGG	0.597339	8_2:-59161866	None:intergenic
CAGGATGAAGACTAGTAGAA+CGG	0.597696	8_2:+59164611	Msa1258060:three_prime_UTR
TGGTAAGGGATCATCGTCGC+CGG	0.598287	8_2:-59161427	None:intergenic
TGAAACAGACTTCCTCCTCT+GGG	0.598630	8_2:+59164780	Msa1258060:three_prime_UTR
CGAGAGAGATCTCGGCGTCG+TGG	0.610673	8_2:-59161456	None:intergenic
TGTAGAGAGAGTGTGTATGG+TGG	0.612108	8_2:-59161332	None:intergenic
CGTCGTGGAGAGATTGGTAA+GGG	0.613592	8_2:-59161441	None:intergenic
GTGTATCAATGGCTTCAACT+TGG	0.614849	8_2:-59162135	None:intergenic
AGATTTCTGCATAATAAATG+GGG	0.616388	8_2:+59161947	Msa1258060:CDS
TCCAGAATTTACAATCCACA+GGG	0.621641	8_2:-59164753	None:intergenic
CAAAATGTAATCATGTGACC+AGG	0.621665	8_2:-59162094	None:intergenic
AGATGATTGGAATTAGGACG+GGG	0.631542	8_2:+59161840	Msa1258060:intron
TTTGAATTGCAACCCAGAGG+AGG	0.632979	8_2:-59164792	None:intergenic
AAGCCATTGATACACACAGC+GGG	0.635131	8_2:+59162143	Msa1258060:CDS
ATTGTGATTACCTTGATGGA+AGG	0.636565	8_2:-59161754	None:intergenic
ATAGCCGGTCCAAGAAAAGA+CGG	0.640967	8_2:-59162067	None:intergenic
AACGAGTGGAAGAGGAACGA+GGG	0.641822	8_2:-59161584	None:intergenic
GATAATATCTCAAATCCAGA+CGG	0.645775	8_2:-59161872	None:intergenic
GTTGAAGAGGACCAACAACA+AGG	0.668088	8_2:-59161720	None:intergenic
TGTCTGTTAACGCAAACAAG+CGG	0.675794	8_2:-59161282	None:intergenic
CTAGAGAGAGAGATTGCCGG+CGG	0.699326	8_2:-59161386	None:intergenic
TAAGAATGATCATTTCCCTG+TGG	0.705862	8_2:+59164738	Msa1258060:three_prime_UTR
ATTCGACAAATGGTGCAGCG+CGG	0.713447	8_2:+59164434	Msa1258060:CDS
TTACATCTATGGAACAGACA+AGG	0.732719	8_2:+59164087	Msa1258060:CDS
GGATGAAGACTAGTAGAACG+GGG	0.748085	8_2:+59164613	Msa1258060:three_prime_UTR
GAAGCCATTGATACACACAG+CGG	0.790862	8_2:+59162142	Msa1258060:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAATATCAACAAAAAA+AGG	-	chr8_2:59162188-59162207	None:intergenic	10.0%
!!	ATAATAAGTTAAATATGAAA+TGG	+	chr8_2:59163141-59163160	Msa1258060:intron	10.0%
!!	ATAATACACAAAAAAATCAA+AGG	-	chr8_2:59162333-59162352	None:intergenic	15.0%
!!!	ATTTAACTTATTATGAGAAA+AGG	-	chr8_2:59163135-59163154	None:intergenic	15.0%
!!!	ATAGATTGTCTTATTATATT+TGG	+	chr8_2:59163655-59163674	Msa1258060:intron	15.0%
!!!	TTTAAAATAGTGATTATATC+TGG	+	chr8_2:59163725-59163744	Msa1258060:intron	15.0%
!!!	TAAAATGGAATATATTTTGT+GGG	-	chr8_2:59163983-59164002	None:intergenic	15.0%
!!!	ATAAAATGGAATATATTTTG+TGG	-	chr8_2:59163984-59164003	None:intergenic	15.0%
!!!	ACAAAATATATTCCATTTTA+TGG	+	chr8_2:59163983-59164002	Msa1258060:CDS	15.0%
!!!	TCAAAAAGCATAATAAAATT+TGG	+	chr8_2:59164810-59164829	Msa1258060:three_prime_UTR	15.0%
!!!	TTAAATTACCGTTTTAGTTA+CGG	+	chr8_2:59161791-59161810	Msa1258060:intron	20.0%
!!	AATTATAACCGTAACTAAAA+CGG	-	chr8_2:59161802-59161821	None:intergenic	20.0%
!!!	TTTTGTTTATTGTAGATGAT+TGG	+	chr8_2:59161827-59161846	Msa1258060:intron	20.0%
!!!	GCAATTATTAGTGTATTTTT+TGG	+	chr8_2:59161895-59161914	Msa1258060:CDS	20.0%
!!!	ATTATTAGTGTATTTTTTGG+TGG	+	chr8_2:59161898-59161917	Msa1258060:CDS	20.0%
!!	ATTTATTATGCAGAAATCTA+TGG	-	chr8_2:59161946-59161965	None:intergenic	20.0%
!!	ATAGATTTCTGCATAATAAA+TGG	+	chr8_2:59161945-59161964	Msa1258060:CDS	20.0%
!!	TAGATTTCTGCATAATAAAT+GGG	+	chr8_2:59161946-59161965	Msa1258060:CDS	20.0%
!!	AAAAAAATCAAAGGAGTAAA+AGG	-	chr8_2:59162324-59162343	None:intergenic	20.0%
!!	TGTTTCTTAGTAAGATTATT+AGG	+	chr8_2:59162388-59162407	Msa1258060:intron	20.0%
!!	GTTTCTTAGTAAGATTATTA+GGG	+	chr8_2:59162389-59162408	Msa1258060:intron	20.0%
!!	GAATATTAGGTAAAAGATAA+AGG	-	chr8_2:59163054-59163073	None:intergenic	20.0%
!!!	CTTTTACCTAATATTCATTT+CGG	+	chr8_2:59163058-59163077	Msa1258060:intron	20.0%
!!	AAACAAAAAAAATGCTAACT+TGG	+	chr8_2:59163164-59163183	Msa1258060:intron	20.0%
!!	CATTTAAAGACAATTTAAAC+GGG	-	chr8_2:59163200-59163219	None:intergenic	20.0%
!!	CCATTTAAAGACAATTTAAA+CGG	-	chr8_2:59163201-59163220	None:intergenic	20.0%
!!	AAGACACAATAATCTAAATA+CGG	-	chr8_2:59163231-59163250	None:intergenic	20.0%
!!	GTTGAAGAAAAAAAATTTGA+AGG	+	chr8_2:59163265-59163284	Msa1258060:intron	20.0%
!!	AAAAATTTGAAGGACAATTT+AGG	+	chr8_2:59163275-59163294	Msa1258060:intron	20.0%
!!	AAAATTTGAAGGACAATTTA+GGG	+	chr8_2:59163276-59163295	Msa1258060:intron	20.0%
!!!	CTTTTTCCTAATAATCATTT+TGG	-	chr8_2:59163433-59163452	None:intergenic	20.0%
!!	GATTATTAGGAAAAAGATAA+AGG	+	chr8_2:59163437-59163456	Msa1258060:intron	20.0%
!!	ATATAGTTGAATGTCAAAAA+TGG	+	chr8_2:59163513-59163532	Msa1258060:intron	20.0%
!!	CAATTGAATGTAAAAATTGT+AGG	+	chr8_2:59163599-59163618	Msa1258060:intron	20.0%
!!	TATCTATCTAATGAAAAAGA+GGG	-	chr8_2:59163857-59163876	None:intergenic	20.0%
!!	ATATCTATCTAATGAAAAAG+AGG	-	chr8_2:59163858-59163877	None:intergenic	20.0%
!!!	AAATATATTCCATTTTATGG+TGG	+	chr8_2:59163986-59164005	Msa1258060:CDS	20.0%
!!	ACTAAACAAAATGAAAAATG+TGG	-	chr8_2:59164341-59164360	None:intergenic	20.0%
!!!	TATATGTCTACAAGAAATTT+TGG	-	chr8_2:59164555-59164574	None:intergenic	20.0%
!	TATAGCAATTAAGCATTTCA+TGG	-	chr8_2:59161678-59161697	None:intergenic	25.0%
!	TATTGTAGATGATTGGAATT+AGG	+	chr8_2:59161834-59161853	Msa1258060:intron	25.0%
!	AGATTTCTGCATAATAAATG+GGG	+	chr8_2:59161947-59161966	Msa1258060:CDS	25.0%
!	ATTACTTTATGCTGAAAGTT+CGG	+	chr8_2:59162643-59162662	Msa1258060:intron	25.0%
!!!	ACATTAAGTGTTCTAAAAAG+AGG	-	chr8_2:59162744-59162763	None:intergenic	25.0%
!	AGTCCAATTAAAAATATTCC+TGG	+	chr8_2:59162794-59162813	Msa1258060:intron	25.0%
!!!	TGTCCAGGAATATTTTTAAT+TGG	-	chr8_2:59162800-59162819	None:intergenic	25.0%
!!	TAGAAACTTTTTACAAGCTT+TGG	+	chr8_2:59162908-59162927	Msa1258060:intron	25.0%
!	TTAAGACCGAAATGAATATT+AGG	-	chr8_2:59163067-59163086	None:intergenic	25.0%
!	CCGTTTAAATTGTCTTTAAA+TGG	+	chr8_2:59163198-59163217	Msa1258060:intron	25.0%
!!!	AAAAAATGTTGACTTAGCAA+TGG	-	chr8_2:59163353-59163372	None:intergenic	25.0%
!	AAAGGACCAAAATGATTATT+AGG	+	chr8_2:59163424-59163443	Msa1258060:intron	25.0%
!	AAAATAGTGATTATATCTGG+TGG	+	chr8_2:59163728-59163747	Msa1258060:intron	25.0%
!	TGTCTGTAGGATTTAATATT+CGG	+	chr8_2:59163799-59163818	Msa1258060:intron	25.0%
!	ATCTATCTAATGAAAAAGAG+GGG	-	chr8_2:59163856-59163875	None:intergenic	25.0%
!	AGCAATCAAACATAAAAGTT+GGG	-	chr8_2:59163923-59163942	None:intergenic	25.0%
!	AAGCAATCAAACATAAAAGT+TGG	-	chr8_2:59163924-59163943	None:intergenic	25.0%
!	ACATATTGTGATTACATCTA+TGG	+	chr8_2:59164076-59164095	Msa1258060:CDS	25.0%
!	AACAAAATGAAAAATGTGGA+AGG	-	chr8_2:59164337-59164356	None:intergenic	25.0%
!	ACTAGTAAAATAGATGTCTT+TGG	+	chr8_2:59164581-59164600	Msa1258060:three_prime_UTR	25.0%
!!!	TGATTTTGTTTTACAAGAGT+GGG	+	chr8_2:59164648-59164667	Msa1258060:three_prime_UTR	25.0%
!!	ATTTTTCTGTTGTCATGTTT+TGG	+	chr8_2:59164682-59164701	Msa1258060:three_prime_UTR	25.0%
	ATGACAGAAGAGAAAGTAAT+CGG	-	chr8_2:59161536-59161555	None:intergenic	30.0%
	TTGATTTCAAGAAAAACGAG+TGG	-	chr8_2:59161601-59161620	None:intergenic	30.0%
!	AAAATGTTCTTCCTTGTTGT+TGG	+	chr8_2:59161709-59161728	Msa1258060:CDS	30.0%
	GATAATATCTCAAATCCAGA+CGG	-	chr8_2:59161875-59161894	None:intergenic	30.0%
!!!	ATTAGTGTATTTTTTGGTGG+AGG	+	chr8_2:59161901-59161920	Msa1258060:CDS	30.0%
!!!	ACATGATTACATTTTGGTTG+TGG	+	chr8_2:59162101-59162120	Msa1258060:CDS	30.0%
	AAAAGGAAAATGACAGAAAG+TGG	-	chr8_2:59162171-59162190	None:intergenic	30.0%
	TTCCAAAAGGACATTCATTA+CGG	-	chr8_2:59162219-59162238	None:intergenic	30.0%
	TTACGTCCAATTGTTTGAAT+GGG	-	chr8_2:59162619-59162638	None:intergenic	30.0%
	ATTACGTCCAATTGTTTGAA+TGG	-	chr8_2:59162620-59162639	None:intergenic	30.0%
	TCATAAACAGGTTTCTTCTA+AGG	-	chr8_2:59162671-59162690	None:intergenic	30.0%
	AAGAAACCTGTTTATGATCT+TGG	+	chr8_2:59162674-59162693	Msa1258060:intron	30.0%
	AGAAACCTGTTTATGATCTT+GGG	+	chr8_2:59162675-59162694	Msa1258060:intron	30.0%
!	TGGAAACAGAATTTTGTTGA+AGG	+	chr8_2:59162715-59162734	Msa1258060:intron	30.0%
	ACTACAGCATAAACTTTCAA+AGG	-	chr8_2:59162825-59162844	None:intergenic	30.0%
	TATGTGAATATGAAGTGAGA+TGG	-	chr8_2:59162985-59163004	None:intergenic	30.0%
!	GATCGATTTCGAAAAGATAA+AGG	-	chr8_2:59163103-59163122	None:intergenic	30.0%
	TACTCACTTGGAAAAGATAA+AGG	+	chr8_2:59163406-59163425	Msa1258060:intron	30.0%
	TAGAAGCGAATTAAAGGAAT+TGG	-	chr8_2:59163547-59163566	None:intergenic	30.0%
	TAACTGTAGAAGCGAATTAA+AGG	-	chr8_2:59163553-59163572	None:intergenic	30.0%
	TTAAATCCTACAGACAAGAT+TGG	-	chr8_2:59163795-59163814	None:intergenic	30.0%
	ATCATGCTGATGTTTATCAA+TGG	-	chr8_2:59163828-59163847	None:intergenic	30.0%
	TCTATCTAATGAAAAAGAGG+GGG	-	chr8_2:59163855-59163874	None:intergenic	30.0%
	TGCTTTACTTATCATATGTC+AGG	+	chr8_2:59163939-59163958	Msa1258060:intron	30.0%
!	ATATATTTTGTGGGATTCCA+GGG	-	chr8_2:59163974-59163993	None:intergenic	30.0%
!	AATATATTTTGTGGGATTCC+AGG	-	chr8_2:59163975-59163994	None:intergenic	30.0%
	TTGCAAATCAATTTCACATG+TGG	-	chr8_2:59164168-59164187	None:intergenic	30.0%
!!!	TTTTGCTTTGTTTATGTCAC+AGG	+	chr8_2:59164191-59164210	Msa1258060:intron	30.0%
!!	TTTGCTTTGTTTATGTCACA+GGG	+	chr8_2:59164192-59164211	Msa1258060:intron	30.0%
	TCAGAAGAAGATACTTCAAA+AGG	+	chr8_2:59164224-59164243	Msa1258060:CDS	30.0%
	CAGCTAAATCAATTCACAAA+AGG	-	chr8_2:59164407-59164426	None:intergenic	30.0%
!	TTGTGAATTGATTTAGCTGA+AGG	+	chr8_2:59164408-59164427	Msa1258060:intron	30.0%
	TGGAAGATCATATAATGAAC+AGG	+	chr8_2:59164459-59164478	Msa1258060:CDS	30.0%
!!!	GTGATTTTGTTTTACAAGAG+TGG	+	chr8_2:59164647-59164666	Msa1258060:three_prime_UTR	30.0%
!!!	TGTTTTGGAATTCGAACATT+CGG	+	chr8_2:59164697-59164716	Msa1258060:three_prime_UTR	30.0%
!	TAATGTAGAGAGAGTGTGTA+TGG	-	chr8_2:59161338-59161357	None:intergenic	35.0%
!	AAAGTAATCGGATTTGGTAG+CGG	-	chr8_2:59161524-59161543	None:intergenic	35.0%
	GAAGAGAAAGTAATCGGATT+TGG	-	chr8_2:59161530-59161549	None:intergenic	35.0%
	TGGAGAGATAAACGAACTTT+GGG	-	chr8_2:59161658-59161677	None:intergenic	35.0%
	ATGGAGAGATAAACGAACTT+TGG	-	chr8_2:59161659-59161678	None:intergenic	35.0%
	ATTGTGATTACCTTGATGGA+AGG	-	chr8_2:59161757-59161776	None:intergenic	35.0%
	TGTGATTGTGATTACCTTGA+TGG	-	chr8_2:59161761-59161780	None:intergenic	35.0%
	GTAGATGATTGGAATTAGGA+CGG	+	chr8_2:59161838-59161857	Msa1258060:intron	35.0%
	TAGATGATTGGAATTAGGAC+GGG	+	chr8_2:59161839-59161858	Msa1258060:intron	35.0%
	TGGAGGATTATACGAATTAC+TGG	+	chr8_2:59161918-59161937	Msa1258060:CDS	35.0%
	CATGAATATCAAGCTCCAAT+TGG	+	chr8_2:59161995-59162014	Msa1258060:CDS	35.0%
	CAAAATGTAATCATGTGACC+AGG	-	chr8_2:59162097-59162116	None:intergenic	35.0%
!	CTGGTCACATGATTACATTT+TGG	+	chr8_2:59162095-59162114	Msa1258060:CDS	35.0%
	AAAGGACATTCATTACGGTT+TGG	-	chr8_2:59162214-59162233	None:intergenic	35.0%
!	AACCGTAATGAATGTCCTTT+TGG	+	chr8_2:59162214-59162233	Msa1258060:intron	35.0%
	ATCTCAAGGATCTGTAATAG+TGG	+	chr8_2:59162249-59162268	Msa1258060:intron	35.0%
	TCTCAAGGATCTGTAATAGT+GGG	+	chr8_2:59162250-59162269	Msa1258060:intron	35.0%
!!!	TGGGTTGCTTTTTGCTTTTA+TGG	+	chr8_2:59162269-59162288	Msa1258060:intron	35.0%
!!!	GGGTTGCTTTTTGCTTTTAT+GGG	+	chr8_2:59162270-59162289	Msa1258060:intron	35.0%
!!!	GGTTGCTTTTTGCTTTTATG+GGG	+	chr8_2:59162271-59162290	Msa1258060:intron	35.0%
	ACAAGCAAAGGTTGTTAAAG+TGG	-	chr8_2:59162468-59162487	None:intergenic	35.0%
	TAACAACCTTTGCTTGTATG+AGG	+	chr8_2:59162471-59162490	Msa1258060:intron	35.0%
!	GATGCTTCAATTTTCTGACA+CGG	+	chr8_2:59162498-59162517	Msa1258060:intron	35.0%
	TGCATTTGGCTGTAAATTGA+TGG	-	chr8_2:59162579-59162598	None:intergenic	35.0%
	GCCAAATGCAGAATTATTGT+CGG	+	chr8_2:59162589-59162608	Msa1258060:intron	35.0%
	ACCGACAATAATTCTGCATT+TGG	-	chr8_2:59162593-59162612	None:intergenic	35.0%
	GAAACCTGTTTATGATCTTG+GGG	+	chr8_2:59162676-59162695	Msa1258060:intron	35.0%
	AAACCTGTTTATGATCTTGG+GGG	+	chr8_2:59162677-59162696	Msa1258060:intron	35.0%
	AAACTTTCAAAGGTTTGTCC+AGG	-	chr8_2:59162815-59162834	None:intergenic	35.0%
	TACACACACACTTACACATA+TGG	+	chr8_2:59163011-59163030	Msa1258060:intron	35.0%
!	GAAGGACAATTTAGGGATTT+TGG	+	chr8_2:59163283-59163302	Msa1258060:intron	35.0%
	TTAGCAATGGTTGTATAAGG+TGG	-	chr8_2:59163340-59163359	None:intergenic	35.0%
	GACTTAGCAATGGTTGTATA+AGG	-	chr8_2:59163343-59163362	None:intergenic	35.0%
	AGTTCATATGCTGTGAGAAA+AGG	+	chr8_2:59163374-59163393	Msa1258060:intron	35.0%
	AGGACAAAAGTTTACTCACT+TGG	+	chr8_2:59163394-59163413	Msa1258060:intron	35.0%
!!	TCAAATTCAAGTTGCAAGCA+CGG	-	chr8_2:59163764-59163783	None:intergenic	35.0%
	TAATTGCCAATCTTGTCTGT+AGG	+	chr8_2:59163786-59163805	Msa1258060:intron	35.0%
	CCAAATTTCAATGCAGAAAC+TGG	-	chr8_2:59163898-59163917	None:intergenic	35.0%
	CCAGTTTCTGCATTGAAATT+TGG	+	chr8_2:59163895-59163914	Msa1258060:intron	35.0%
!	TATATTTTGTGGGATTCCAG+GGG	-	chr8_2:59163973-59163992	None:intergenic	35.0%
	CACGATTACCATCATTATGT+TGG	+	chr8_2:59164019-59164038	Msa1258060:CDS	35.0%
	GATTACCATCATTATGTTGG+AGG	+	chr8_2:59164022-59164041	Msa1258060:CDS	35.0%
	TTTGTCCTCCAACATAATGA+TGG	-	chr8_2:59164030-59164049	None:intergenic	35.0%
	AAACTGATGCAAAATTGCTC+TGG	-	chr8_2:59164054-59164073	None:intergenic	35.0%
	TTACATCTATGGAACAGACA+AGG	+	chr8_2:59164087-59164106	Msa1258060:CDS	35.0%
!	TGTTTATGTCACAGGGTTTT+CGG	+	chr8_2:59164199-59164218	Msa1258060:intron	35.0%
	AAGCTAATTAAAGCCACAAC+AGG	-	chr8_2:59164288-59164307	None:intergenic	35.0%
	TCACAAAAGGACAATGAAGT+GGG	-	chr8_2:59164394-59164413	None:intergenic	35.0%
	TTCACAAAAGGACAATGAAG+TGG	-	chr8_2:59164395-59164414	None:intergenic	35.0%
!!!	GAATGAGACAGAAGCATTTT+AGG	+	chr8_2:59164496-59164515	Msa1258060:exon	35.0%
!!!	TTTGTTTTACAAGAGTGGGT+TGG	+	chr8_2:59164652-59164671	Msa1258060:three_prime_UTR	35.0%
!!	TTGTTTTACAAGAGTGGGTT+GGG	+	chr8_2:59164653-59164672	Msa1258060:three_prime_UTR	35.0%
	TAAGAATGATCATTTCCCTG+TGG	+	chr8_2:59164738-59164757	Msa1258060:three_prime_UTR	35.0%
	TCCAGAATTTACAATCCACA+GGG	-	chr8_2:59164756-59164775	None:intergenic	35.0%
	TTCCAGAATTTACAATCCAC+AGG	-	chr8_2:59164757-59164776	None:intergenic	35.0%
	TGTCTGTTAACGCAAACAAG+CGG	-	chr8_2:59161285-59161304	None:intergenic	40.0%
!!!	TCTCTAGGGTTTCTTTTCTC+AGG	+	chr8_2:59161402-59161421	Msa1258060:five_prime_UTR	40.0%
	GTAGTTGAACCATAGGGTTT+CGG	-	chr8_2:59161500-59161519	None:intergenic	40.0%
	TCAAGAAAAACGAGTGGAAG+AGG	-	chr8_2:59161595-59161614	None:intergenic	40.0%
	GGATCTTGTACTCATCGAAA+GGG	-	chr8_2:59161633-59161652	None:intergenic	40.0%
	TGGATCTTGTACTCATCGAA+AGG	-	chr8_2:59161634-59161653	None:intergenic	40.0%
!	GGATAAGAAACGAGTTGAAG+AGG	-	chr8_2:59161736-59161755	None:intergenic	40.0%
	CGTTTCTTATCCTTCCATCA+AGG	+	chr8_2:59161744-59161763	Msa1258060:CDS	40.0%
	AGATGATTGGAATTAGGACG+GGG	+	chr8_2:59161840-59161859	Msa1258060:intron	40.0%
	ATCTCAAATCCAGACGGTAA+CGG	-	chr8_2:59161869-59161888	None:intergenic	40.0%
	TCAAGCTCCAATTGGATTTG+CGG	+	chr8_2:59162003-59162022	Msa1258060:CDS	40.0%
	GTGTATCAATGGCTTCAACT+TGG	-	chr8_2:59162138-59162157	None:intergenic	40.0%
!!!	TGTCCTTTTGGAAAGTGCTT+TGG	+	chr8_2:59162226-59162245	Msa1258060:intron	40.0%
!	GATCCAAAGCACTTTCCAAA+AGG	-	chr8_2:59162232-59162251	None:intergenic	40.0%
	TGCAACTATAGATCTCACTC+AGG	-	chr8_2:59162297-59162316	None:intergenic	40.0%
	AGCTGATACAGTTGCAATTG+CGG	+	chr8_2:59162423-59162442	Msa1258060:intron	40.0%
	ATACAGTTGCAATTGCGGTT+GGG	+	chr8_2:59162428-59162447	Msa1258060:intron	40.0%
	TCACAACCTCATACAAGCAA+AGG	-	chr8_2:59162480-59162499	None:intergenic	40.0%
	GGTTCACCCATTCAAACAAT+TGG	+	chr8_2:59162610-59162629	Msa1258060:intron	40.0%
	AACCTGTTTATGATCTTGGG+GGG	+	chr8_2:59162678-59162697	Msa1258060:intron	40.0%
	CACCAAGACGTAAATCTTTC+CGG	+	chr8_2:59162865-59162884	Msa1258060:intron	40.0%
!	TAGGGATTTTGGATGACAGT+TGG	+	chr8_2:59163294-59163313	Msa1258060:intron	40.0%
!	AGGGATTTTGGATGACAGTT+GGG	+	chr8_2:59163295-59163314	Msa1258060:intron	40.0%
!!	GTTAGTGATTAGTCTTGCTG+AGG	+	chr8_2:59163677-59163696	Msa1258060:intron	40.0%
	CAATCCACCAGATATAAGCA+CGG	-	chr8_2:59163703-59163722	None:intergenic	40.0%
	TTATATCTGGTGGAATGTCC+AGG	+	chr8_2:59163738-59163757	Msa1258060:intron	40.0%
	ATGCAGAAACTGGAGAAGAA+TGG	-	chr8_2:59163888-59163907	None:intergenic	40.0%
!!	TGGAAGGTGTTTGAGCAAAA+TGG	-	chr8_2:59164321-59164340	None:intergenic	40.0%
	CACAAAAGGACAATGAAGTG+GGG	-	chr8_2:59164393-59164412	None:intergenic	40.0%
	CTGAAGGAAGATTCGACAAA+TGG	+	chr8_2:59164424-59164443	Msa1258060:CDS	40.0%
!!!	TGAGACAGAAGCATTTTAGG+TGG	+	chr8_2:59164499-59164518	Msa1258060:exon	40.0%
	AGGTGGTGTATGTTGTATGT+TGG	+	chr8_2:59164516-59164535	Msa1258060:three_prime_UTR	40.0%
!!	AGATGTCTTTGGTTAGCTTC+AGG	+	chr8_2:59164592-59164611	Msa1258060:three_prime_UTR	40.0%
	CAGGATGAAGACTAGTAGAA+CGG	+	chr8_2:59164611-59164630	Msa1258060:three_prime_UTR	40.0%
	AGGATGAAGACTAGTAGAAC+GGG	+	chr8_2:59164612-59164631	Msa1258060:three_prime_UTR	40.0%
!!!	TGTTTTACAAGAGTGGGTTG+GGG	+	chr8_2:59164654-59164673	Msa1258060:three_prime_UTR	40.0%
!	TCCCTGTGGATTGTAAATTC+TGG	+	chr8_2:59164752-59164771	Msa1258060:three_prime_UTR	40.0%
!	CTTTTTGAATTGCAACCCAG+AGG	-	chr8_2:59164798-59164817	None:intergenic	40.0%
!	TGTAGAGAGAGTGTGTATGG+TGG	-	chr8_2:59161335-59161354	None:intergenic	45.0%
	AGCGGAGTAGTTGAACCATA+GGG	-	chr8_2:59161506-59161525	None:intergenic	45.0%
	TAGCGGAGTAGTTGAACCAT+AGG	-	chr8_2:59161507-59161526	None:intergenic	45.0%
	GAGATAAACGAACTTTGGGC+TGG	-	chr8_2:59161654-59161673	None:intergenic	45.0%
	GTTGAAGAGGACCAACAACA+AGG	-	chr8_2:59161723-59161742	None:intergenic	45.0%
!	TTGGGCGGAGATCTTGATTT+TGG	+	chr8_2:59162039-59162058	Msa1258060:CDS	45.0%
!	TGGGCGGAGATCTTGATTTT+GGG	+	chr8_2:59162040-59162059	Msa1258060:CDS	45.0%
!	TTGGGGATTCCGTCTTTTCT+TGG	+	chr8_2:59162058-59162077	Msa1258060:CDS	45.0%
!	GATTCCGTCTTTTCTTGGAC+CGG	+	chr8_2:59162063-59162082	Msa1258060:CDS	45.0%
	ATAGCCGGTCCAAGAAAAGA+CGG	-	chr8_2:59162070-59162089	None:intergenic	45.0%
	GAAGCCATTGATACACACAG+CGG	+	chr8_2:59162142-59162161	Msa1258060:CDS	45.0%
	AAGCCATTGATACACACAGC+GGG	+	chr8_2:59162143-59162162	Msa1258060:CDS	45.0%
!!	GGAAAGTGCTTTGGATCTCA+AGG	+	chr8_2:59162235-59162254	Msa1258060:intron	45.0%
	GATACAGTTGCAATTGCGGT+TGG	+	chr8_2:59162427-59162446	Msa1258060:intron	45.0%
	ACCTGTTTATGATCTTGGGG+GGG	+	chr8_2:59162679-59162698	Msa1258060:intron	45.0%
	TGCCGGAAAGATTTACGTCT+TGG	-	chr8_2:59162870-59162889	None:intergenic	45.0%
!	TTCCGGCATTTTCTGTCTCA+AGG	+	chr8_2:59162882-59162901	Msa1258060:intron	45.0%
!	TCCGGCATTTTCTGTCTCAA+GGG	+	chr8_2:59162883-59162902	Msa1258060:intron	45.0%
	ACCCTTGAGACAGAAAATGC+CGG	-	chr8_2:59162887-59162906	None:intergenic	45.0%
	TTTGGATGACAGTTGGGAGT+TGG	+	chr8_2:59163301-59163320	Msa1258060:intron	45.0%
	TTGGATGACAGTTGGGAGTT+GGG	+	chr8_2:59163302-59163321	Msa1258060:intron	45.0%
	AAGCCACAACAGGCAAATCA+CGG	-	chr8_2:59164278-59164297	None:intergenic	45.0%
	GGATGAAGACTAGTAGAACG+GGG	+	chr8_2:59164613-59164632	Msa1258060:three_prime_UTR	45.0%
	TGAAACAGACTTCCTCCTCT+GGG	+	chr8_2:59164780-59164799	Msa1258060:three_prime_UTR	45.0%
	TTTGAATTGCAACCCAGAGG+AGG	-	chr8_2:59164795-59164814	None:intergenic	45.0%
	ACCCTAGAGAGAGAGATTGC+CGG	-	chr8_2:59161392-59161411	None:intergenic	50.0%
	CGTCGTGGAGAGATTGGTAA+GGG	-	chr8_2:59161444-59161463	None:intergenic	50.0%
	ATTGCGACCGAGAGAGATCT+CGG	-	chr8_2:59161467-59161486	None:intergenic	50.0%
	AACGAGTGGAAGAGGAACGA+GGG	-	chr8_2:59161587-59161606	None:intergenic	50.0%
	AAACGAGTGGAAGAGGAACG+AGG	-	chr8_2:59161588-59161607	None:intergenic	50.0%
!!	TAAGGCGCCGCAAATCCAAT+TGG	-	chr8_2:59162013-59162032	None:intergenic	50.0%
	ATCTCCGCCCAATGTGCATA+AGG	-	chr8_2:59162031-59162050	None:intergenic	50.0%
!	GGGCGGAGATCTTGATTTTG+GGG	+	chr8_2:59162041-59162060	Msa1258060:CDS	50.0%
!	GTCTTTTCTTGGACCGGCTA+TGG	+	chr8_2:59162069-59162088	Msa1258060:CDS	50.0%
	ATGTGACCAGGAACCATAGC+CGG	-	chr8_2:59162085-59162104	None:intergenic	50.0%
	GTACCCGCTGTGTGTATCAA+TGG	-	chr8_2:59162149-59162168	None:intergenic	50.0%
	ACCCCCCCAAGATCATAAAC+AGG	-	chr8_2:59162683-59162702	None:intergenic	50.0%
	TGTTTATGATCTTGGGGGGG+TGG	+	chr8_2:59162682-59162701	Msa1258060:intron	50.0%
	GTTTATGATCTTGGGGGGGT+GGG	+	chr8_2:59162683-59162702	Msa1258060:intron	50.0%
	TTTATGATCTTGGGGGGGTG+GGG	+	chr8_2:59162684-59162703	Msa1258060:intron	50.0%
	ATGTCAGGTATGACTTCCCC+TGG	+	chr8_2:59163954-59163973	Msa1258060:intron	50.0%
	TGCTCAGCGCCACCATAAAA+TGG	-	chr8_2:59163998-59164017	None:intergenic	50.0%
!	GAACCGTGATTTGCCTGTTG+TGG	+	chr8_2:59164272-59164291	Msa1258060:intron	50.0%
	ATTCGACAAATGGTGCAGCG+CGG	+	chr8_2:59164434-59164453	Msa1258060:CDS	50.0%
	GTGAAACAGACTTCCTCCTC+TGG	+	chr8_2:59164779-59164798	Msa1258060:three_prime_UTR	50.0%
!!	GTGTATGGTGGATGATGCGC+TGG	-	chr8_2:59161323-59161342	None:intergenic	55.0%
	CTAGAGAGAGAGATTGCCGG+CGG	-	chr8_2:59161389-59161408	None:intergenic	55.0%
	GCCGGCAATCTCTCTCTCTA+GGG	+	chr8_2:59161388-59161407	Msa1258060:five_prime_UTR	55.0%
!	GGGTTTCTTTTCTCAGGCGC+CGG	+	chr8_2:59161408-59161427	Msa1258060:five_prime_UTR	55.0%
	TGGTAAGGGATCATCGTCGC+CGG	-	chr8_2:59161430-59161449	None:intergenic	55.0%
	GCGTCGTGGAGAGATTGGTA+AGG	-	chr8_2:59161445-59161464	None:intergenic	55.0%
	TCACCTTCGCCGAAACCCTA+TGG	+	chr8_2:59161488-59161507	Msa1258060:CDS	55.0%
	GAACCATAGGGTTTCGGCGA+AGG	-	chr8_2:59161494-59161513	None:intergenic	55.0%
	CGAGGGAGAAGATGAGGAAG+AGG	-	chr8_2:59161570-59161589	None:intergenic	55.0%
	GAGGAACGAGGGAGAAGATG+AGG	-	chr8_2:59161576-59161595	None:intergenic	55.0%
	TTGCGGCGCCTTATGCACAT+TGG	+	chr8_2:59162020-59162039	Msa1258060:CDS	55.0%
	TGCGGCGCCTTATGCACATT+GGG	+	chr8_2:59162021-59162040	Msa1258060:CDS	55.0%
	TTATGATCTTGGGGGGGTGG+GGG	+	chr8_2:59162685-59162704	Msa1258060:intron	55.0%
	GCTGAGGCCGTGCTTATATC+TGG	+	chr8_2:59163693-59163712	Msa1258060:intron	55.0%
	GAGGCCGTGCTTATATCTGG+TGG	+	chr8_2:59163696-59163715	Msa1258060:intron	55.0%
!!	TTCAAGTTGCAAGCACGGCC+TGG	-	chr8_2:59163759-59163778	None:intergenic	55.0%
	ACAAATGGTGCAGCGCGGAA+TGG	+	chr8_2:59164439-59164458	Msa1258060:CDS	55.0%
!	GATGCGCTGGACTGGATGGA+TGG	-	chr8_2:59161310-59161329	None:intergenic	60.0%
!	GGATGATGCGCTGGACTGGA+TGG	-	chr8_2:59161314-59161333	None:intergenic	60.0%
!	TGGTGGATGATGCGCTGGAC+TGG	-	chr8_2:59161318-59161337	None:intergenic	60.0%
	CGCCGGCAATCTCTCTCTCT+AGG	+	chr8_2:59161387-59161406	Msa1258060:five_prime_UTR	60.0%
	TCTCGGCGTCGTGGAGAGAT+TGG	-	chr8_2:59161450-59161469	None:intergenic	60.0%
	CACGACGCCGAGATCTCTCT+CGG	+	chr8_2:59161457-59161476	Msa1258060:CDS	60.0%
	ACGGGGTTACCGTTACCGTC+TGG	+	chr8_2:59161857-59161876	Msa1258060:CDS	60.0%
	GGCGCCTTATGCACATTGGG+CGG	+	chr8_2:59162024-59162043	Msa1258060:CDS	60.0%
	CTTGGACCGGCTATGGTTCC+TGG	+	chr8_2:59162076-59162095	Msa1258060:CDS	60.0%
	TATGATCTTGGGGGGGTGGG+GGG	+	chr8_2:59162686-59162705	Msa1258060:intron	60.0%
	AGAGAGAGATTGCCGGCGGC+GGG	-	chr8_2:59161385-59161404	None:intergenic	65.0%
	GAGAGAGAGATTGCCGGCGG+CGG	-	chr8_2:59161386-59161405	None:intergenic	65.0%
	CGAGAGAGATCTCGGCGTCG+TGG	-	chr8_2:59161459-59161478	None:intergenic	65.0%
	AGATTGCCGGCGGCGGGAGA+GGG	-	chr8_2:59161379-59161398	None:intergenic	70.0%
	GATTGCCGGCGGCGGGAGAG+GGG	-	chr8_2:59161378-59161397	None:intergenic	75.0%
	GAGATTGCCGGCGGCGGGAG+AGG	-	chr8_2:59161380-59161399	None:intergenic	75.0%
!	CGGCGGCGGGAGAGGGGAGA+GGG	-	chr8_2:59161372-59161391	None:intergenic	80.0%
!	CCTCTCCCCTCTCCCGCCGC+CGG	+	chr8_2:59161370-59161389	Msa1258060:five_prime_UTR	80.0%
!	GGGGGGGTGGGGGGCTCTGT+TGG	+	chr8_2:59162695-59162714	Msa1258060:intron	80.0%
!	CCGGCGGCGGGAGAGGGGAG+AGG	-	chr8_2:59161373-59161392	None:intergenic	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	59161272	59164840	59161272	ID=Msa1258060;Name=Msa1258060
chr8_2	mRNA	59161272	59164840	59161272	ID=Msa1258060-mRNA-1;Parent=Msa1258060;Name=Msa1258060-mRNA-1;_AED=0.02;_eAED=0.02;_QI=164\|1\|1\|1\|1\|1\|5\|339\|303
chr8_2	exon	59161272	59161765	59161272	ID=Msa1258060-mRNA-1:exon:18312;Parent=Msa1258060-mRNA-1
chr8_2	exon	59161842	59162164	59161842	ID=Msa1258060-mRNA-1:exon:18313;Parent=Msa1258060-mRNA-1
chr8_2	exon	59163961	59164108	59163961	ID=Msa1258060-mRNA-1:exon:18314;Parent=Msa1258060-mRNA-1
chr8_2	exon	59164213	59164245	59164213	ID=Msa1258060-mRNA-1:exon:18315;Parent=Msa1258060-mRNA-1
chr8_2	exon	59164424	59164840	59164424	ID=Msa1258060-mRNA-1:exon:18316;Parent=Msa1258060-mRNA-1
chr8_2	five_prime_UTR	59161272	59161435	59161272	ID=Msa1258060-mRNA-1:five_prime_utr;Parent=Msa1258060-mRNA-1
chr8_2	CDS	59161436	59161765	59161436	ID=Msa1258060-mRNA-1:cds;Parent=Msa1258060-mRNA-1
chr8_2	CDS	59161842	59162164	59161842	ID=Msa1258060-mRNA-1:cds;Parent=Msa1258060-mRNA-1
chr8_2	CDS	59163961	59164108	59163961	ID=Msa1258060-mRNA-1:cds;Parent=Msa1258060-mRNA-1
chr8_2	CDS	59164213	59164245	59164213	ID=Msa1258060-mRNA-1:cds;Parent=Msa1258060-mRNA-1
chr8_2	CDS	59164424	59164501	59164424	ID=Msa1258060-mRNA-1:cds;Parent=Msa1258060-mRNA-1
chr8_2	three_prime_UTR	59164502	59164840	59164502	ID=Msa1258060-mRNA-1:three_prime_utr;Parent=Msa1258060-mRNA-1

Gene Sequence

>Msa1258060

ATGATCCCTTACCAATCTCTCCACGACGCCGAGATCTCTCTCGGTCGCAATCTCACCTTCGCCGAAACCCTATGGTTCAACTACTCCGCTACCAAATCCGATTACTTTCTCTTCTGTCATAACATTCTCTTCCTCTTCCTCATCTTCTCCCTCGTTCCTCTTCCACTCGTTTTTCTTGAAATCAAACGCTTATACCCTTTCGATGAGTACAAGATCCAGCCCAAAGTTCGTTTATCTCTCCATGAAATGCTTAATTGCTATAAAGATGTCTTGAAAATGTTCTTCCTTGTTGTTGGTCCTCTTCAACTCGTTTCTTATCCTTCCATCAAGATGATTGGAATTAGGACGGGGTTACCGTTACCGTCTGGATTTGAGATATTATCGCAATTATTAGTGTATTTTTTGGTGGAGGATTATACGAATTACTGGATCCATAGATTTCTGCATAATAAATGGGGTTATGAGAAGATTCATCGAGTTCATCATGAATATCAAGCTCCAATTGGATTTGCGGCGCCTTATGCACATTGGGCGGAGATCTTGATTTTGGGGATTCCGTCTTTTCTTGGACCGGCTATGGTTCCTGGTCACATGATTACATTTTGGTTGTGGATTGCATTGCGCCAAGTTGAAGCCATTGATACACACAGCGGGTATGACTTCCCCTGGAATCCCACAAAATATATTCCATTTTATGGTGGCGCTGAGCATCACGATTACCATCATTATGTTGGAGGACAAAGCCAGAGCAATTTTGCATCAGTTTTCACATATTGTGATTACATCTATGGAACAGACAAGGGTTTTCGGTATCAGAAGAAGATACTTCAAAAGCTGAAGGAAGATTCGACAAATGGTGCAGCGCGGAATGGAAGATCATATAATGAACAGGACAATTATAAATCTGAATGA

Protein sequence

>Msa1258060

MIPYQSLHDAEISLGRNLTFAETLWFNYSATKSDYFLFCHNILFLFLIFSLVPLPLVFLEIKRLYPFDEYKIQPKVRLSLHEMLNCYKDVLKMFFLVVGPLQLVSYPSIKMIGIRTGLPLPSGFEILSQLLVYFLVEDYTNYWIHRFLHNKWGYEKIHRVHHEYQAPIGFAAPYAHWAEILILGIPSFLGPAMVPGHMITFWLWIALRQVEAIDTHSGYDFPWNPTKYIPFYGGAEHHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGFRYQKKILQKLKEDSTNGAARNGRSYNEQDNYKSE*