AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005035

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005035.t1 MTR_1g079410 87.153 288 23 2 1 288 1 274 0.0 503
MS.gene005035.t1 MTR_1g079400 68.874 302 50 3 2 287 6 279 2.70e-130 371
MS.gene005035.t1 MTR_1g079260 56.701 291 99 5 6 288 2 273 5.37e-106 309
MS.gene005035.t1 MTR_1g079370 56.537 283 103 4 6 287 2 265 3.45e-103 302
MS.gene005035.t1 MTR_1g100597 56.944 288 95 5 9 287 5 272 5.51e-102 299
MS.gene005035.t1 MTR_1g079380 55.124 283 107 4 6 287 2 265 2.67e-97 288
MS.gene005035.t1 MTR_1g079460 55.830 283 102 5 9 288 5 267 9.36e-97 286
MS.gene005035.t1 MTR_1g079180 54.417 283 103 7 9 287 5 265 9.46e-91 270
MS.gene005035.t1 MTR_1g079200 52.817 284 105 6 9 287 5 264 7.84e-90 268
MS.gene005035.t1 MTR_1g079230 51.890 291 112 5 6 287 2 273 1.16e-87 263
MS.gene005035.t1 MTR_1g079850 49.495 297 118 6 7 288 3 282 1.18e-81 248
MS.gene005035.t1 MTR_3g452600 50.000 280 118 5 8 287 10 267 3.47e-80 243
MS.gene005035.t1 MTR_1g100613 53.282 259 90 5 24 270 5 244 8.38e-80 242
MS.gene005035.t1 MTR_1g079140 44.969 318 125 5 2 287 7 306 7.97e-77 236
MS.gene005035.t1 MTR_1g026510 46.154 299 127 9 7 288 3 284 1.12e-73 228
MS.gene005035.t1 MTR_2g079100 48.592 284 110 7 7 284 8 261 1.71e-73 226
MS.gene005035.t1 MTR_3g006850 46.226 318 114 9 9 288 18 316 1.98e-73 228
MS.gene005035.t1 MTR_1g079250 46.102 295 128 7 7 288 3 279 2.36e-69 217
MS.gene005035.t1 MTR_3g450990 44.333 300 121 7 8 287 10 283 6.74e-69 215
MS.gene005035.t1 MTR_1g083580 40.203 296 143 8 6 287 6 281 2.53e-65 206
MS.gene005035.t1 MTR_7g112550 42.667 300 133 11 6 287 5 283 3.99e-65 206
MS.gene005035.t1 MTR_1g079350 44.983 289 137 6 6 287 2 275 2.08e-62 198
MS.gene005035.t1 MTR_1g079340 43.878 294 138 7 9 287 44 325 6.45e-57 186
MS.gene005035.t1 MTR_7g096300 47.212 269 107 9 6 267 2 242 5.15e-54 187
MS.gene005035.t1 MTR_7g112560 38.078 281 149 6 8 287 1 257 7.22e-53 173
MS.gene005035.t1 MTR_1g078470 56.180 178 71 5 111 287 13 184 9.24e-53 171
MS.gene005035.t1 MTR_4g023570 35.231 281 137 4 8 287 1 237 2.49e-45 153
MS.gene005035.t1 MTR_1g079240 46.597 191 88 4 98 287 2 179 1.45e-42 144
MS.gene005035.t1 MTR_1g079130 44.041 193 77 4 122 287 1 189 1.34e-41 142
MS.gene005035.t1 MTR_1g076870 51.471 136 47 2 149 267 2 135 1.38e-33 120
MS.gene005035.t1 MTR_1g079350 40.462 173 78 5 6 173 2 154 9.23e-27 104
MS.gene005035.t1 MTR_1g079210 48.387 124 49 2 151 261 8 129 1.65e-23 93.6
MS.gene005035.t1 MTR_7g096310 42.748 131 58 4 75 203 44 159 1.18e-21 89.7
MS.gene005035.t1 MTR_3g058640 50.485 103 48 2 149 251 7 106 2.20e-21 87.0
MS.gene005035.t1 MTR_4g083970 27.751 209 126 4 84 287 102 290 2.21e-20 89.0
MS.gene005035.t1 MTR_2g042540 42.241 116 52 2 9 124 5 105 8.57e-20 83.6
MS.gene005035.t1 MTR_1g078460 52.326 86 37 1 7 92 66 147 6.72e-19 81.6
MS.gene005035.t1 MTR_7g103380 24.315 292 179 8 11 288 22 285 6.41e-18 82.4
MS.gene005035.t1 MTR_4g102770 32.484 157 91 3 11 167 22 163 8.42e-16 76.3
MS.gene005035.t1 MTR_1g101390 31.847 157 91 3 11 167 31 171 4.05e-15 74.3
MS.gene005035.t1 MTR_0113s0040 27.607 163 102 3 5 167 19 165 5.45e-14 70.9
MS.gene005035.t1 MTR_0002s0020 28.571 161 98 2 11 168 25 171 7.50e-14 70.5
MS.gene005035.t1 MTR_1g079220 50.725 69 31 2 186 254 1 66 1.46e-13 65.1
MS.gene005035.t1 MTR_5g010800 26.829 164 102 3 6 168 15 161 5.36e-13 68.2
MS.gene005035.t1 MTR_1g079390 68.000 50 14 2 233 282 8 55 1.15e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005035.t1 AT2G02360 42.612 291 137 6 1 288 7 270 1.16e-72 224
MS.gene005035.t1 AT2G02230 37.615 327 138 9 6 288 30 334 9.91e-68 214
MS.gene005035.t1 AT2G02250 41.754 285 140 7 6 288 45 305 1.48e-65 207
MS.gene005035.t1 AT2G02240 41.549 284 141 5 6 288 59 318 6.33e-62 199
MS.gene005035.t1 AT2G02340 40.559 286 147 6 6 286 34 301 6.61e-61 196
MS.gene005035.t1 AT1G80110 41.877 277 135 7 11 286 4 255 2.45e-59 190
MS.gene005035.t1 AT2G02350 37.979 287 150 6 6 288 22 284 1.83e-54 179
MS.gene005035.t1 AT1G09155 39.661 295 143 8 11 287 3 280 4.15e-54 177
MS.gene005035.t1 AT1G56240 39.796 294 142 8 11 286 4 280 3.33e-51 170
MS.gene005035.t1 AT1G56250 40.678 295 140 9 11 287 4 281 1.63e-50 168
MS.gene005035.t1 AT2G02320 37.631 287 141 8 11 288 43 300 1.65e-46 158
MS.gene005035.t1 AT2G02310 34.737 285 160 6 6 287 43 304 4.60e-45 155
MS.gene005035.t1 AT2G02300 34.828 290 141 10 6 287 32 281 6.09e-40 140
MS.gene005035.t1 AT5G24560 46.250 160 66 2 11 170 6 145 1.33e-39 137
MS.gene005035.t1 AT2G02350 34.503 171 104 4 122 288 1 167 1.37e-23 95.1
MS.gene005035.t1 AT3G53000 25.342 292 171 9 11 286 24 284 6.71e-18 82.4
MS.gene005035.t1 AT4G19850 31.694 183 96 6 113 287 15 176 3.99e-17 77.8
MS.gene005035.t1 AT4G19850 31.694 183 96 6 113 287 57 218 6.03e-17 78.2
MS.gene005035.t1 AT1G12710 31.098 164 96 3 5 168 25 171 2.19e-14 72.4
MS.gene005035.t1 AT3G61060 29.677 155 94 2 11 165 28 167 4.75e-14 71.2
MS.gene005035.t1 AT1G65390 27.684 177 105 4 115 287 253 410 1.04e-13 71.2
MS.gene005035.t1 AT5G52120 30.909 165 96 3 8 168 21 171 1.12e-13 70.1
MS.gene005035.t1 AT3G61060 29.677 155 93 2 11 165 28 166 1.68e-13 69.7
MS.gene005035.t1 AT1G63090 31.013 158 92 3 11 168 29 169 2.52e-13 69.3
MS.gene005035.t1 AT4G19840 24.887 221 142 5 73 288 45 246 4.44e-13 67.8
MS.gene005035.t1 AT1G33920 27.322 183 94 5 117 287 9 164 3.84e-12 63.5
MS.gene005035.t1 AT2G26820 23.316 193 124 4 103 286 284 461 3.46e-11 63.9
MS.gene005035.t1 AT2G26820 24.855 173 111 3 118 286 320 477 6.19e-11 63.2

Find 81 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTGCTGGTTTGAAATTTA+TGG 0.100064 1.4:-64200189 MS.gene005035:CDS
AGTGAGAAGTGATGGATTTC+TGG 0.210364 1.4:-64199950 MS.gene005035:CDS
AATCCATCACTTCTCACTTT+TGG 0.235555 1.4:+64199955 None:intergenic
TTAAACTTGACTCTTTATTA+TGG 0.262984 1.4:-64200476 MS.gene005035:CDS
CGTTCTCTATCATCATAGTC+TGG 0.266145 1.4:+64200006 None:intergenic
TCTCAACAACTTCATCTTCT+AGG 0.271024 1.4:+64199896 None:intergenic
TACGAAGTCTAGCAACTTCT+TGG 0.288075 1.4:+64200214 None:intergenic
GGGTGACACTCCTTGCTATT+GGG 0.294051 1.4:-64200366 MS.gene005035:CDS
AACTCGATCCTTCACTTCTT+AGG 0.308997 1.4:+64199805 None:intergenic
CCGCTGAATCAGATATTGTT+TGG 0.317627 1.4:-64200712 MS.gene005035:CDS
ATGGGAGAGTTCTTCAATTC+AGG 0.321358 1.4:-64199919 MS.gene005035:CDS
TCTGCTGGTTTGAAATTTAT+GGG 0.342537 1.4:-64200188 MS.gene005035:CDS
GGGGTGACACTCCTTGCTAT+TGG 0.354208 1.4:-64200367 MS.gene005035:CDS
TTTACCATTGTTGATTATGA+TGG 0.358804 1.4:+64200574 None:intergenic
AGAGAAACGGAGTGGTAAAA+AGG 0.359870 1.4:-64200426 MS.gene005035:CDS
AGTGAGATATAGAGACTTCT+TGG 0.362866 1.4:+64200610 None:intergenic
GATGGGTGTACTTGAAATAA+AGG 0.367176 1.4:-64199875 MS.gene005035:CDS
CGCTGAATCAGATATTGTTT+GGG 0.389539 1.4:-64200711 MS.gene005035:CDS
AGCGGCAGAACAAAAGCCTT+TGG 0.405094 1.4:+64200730 None:intergenic
CTAGACTTCGTACGGTCTGC+TGG 0.413537 1.4:-64200203 MS.gene005035:CDS
CTAGAGATCTCTCCATTACT+TGG 0.414237 1.4:-64200388 MS.gene005035:CDS
AAAGGTGGTTTCATTCTTGA+AGG 0.434634 1.4:-64199841 MS.gene005035:CDS
TGGGTCCAACCAAACATTCT+TGG 0.440943 1.4:+64200026 None:intergenic
ACACTCCTTGCTATTGGGAT+TGG 0.441059 1.4:-64200361 MS.gene005035:CDS
AAGTTAACTGTGGGTGTACT+TGG 0.444694 1.4:-64200069 MS.gene005035:CDS
AAGCACGATAGAGGCTTTGC+CGG 0.461575 1.4:-64200819 MS.gene005035:CDS
AGCTACGTATTGAGTATTTG+AGG 0.465011 1.4:+64200143 None:intergenic
TTACCATTGTTGATTATGAT+GGG 0.474095 1.4:+64200575 None:intergenic
AGAGCTTTCAATTAGAGAAA+CGG 0.476839 1.4:-64200439 MS.gene005035:CDS
GTGTATCTACCAAGAATGTT+TGG 0.478752 1.4:-64200035 MS.gene005035:CDS
TAGAGATCTCTCCATTACTT+GGG 0.480318 1.4:-64200387 MS.gene005035:CDS
TTGAAAGCTCTGAAATTTAG+CGG 0.493619 1.4:+64200451 None:intergenic
AGAAATGATTGAGATCAAAT+CGG 0.495855 1.4:+64200673 None:intergenic
AGTTGTATGCGACAATATCT+TGG 0.496653 1.4:+64200784 None:intergenic
GTTCTCTATCATCATAGTCT+GGG 0.511952 1.4:+64200007 None:intergenic
AAGTACACCCACAGTTAACT+TGG 0.516811 1.4:+64200071 None:intergenic
CGTCCAAAAGTGAGAAGTGA+TGG 0.519993 1.4:-64199958 MS.gene005035:CDS
GAGATATAGAGACTTCTTGG+AGG 0.521963 1.4:+64200613 None:intergenic
TAAACAGTGAGGACGCAGAC+TGG 0.534129 1.4:+64200645 None:intergenic
ATCTGACCTGGACTCTGGTA+GGG 0.540951 1.4:+64200334 None:intergenic
ATGATTGAGATCAAATCGGA+TGG 0.542290 1.4:+64200677 None:intergenic
TAGAGACTTCTTGGAGGGAG+AGG 0.543893 1.4:+64200619 None:intergenic
AGGAGTGTCACCCCAAGTAA+TGG 0.550622 1.4:+64200376 None:intergenic
ATGGGTGTACTTGAAATAAA+GGG 0.553967 1.4:-64199874 MS.gene005035:CDS
ATCTACCAAGAATGTTTGGT+TGG 0.558152 1.4:-64200031 MS.gene005035:CDS
ATCTTGGCAATGCATCCCTC+CGG 0.558404 1.4:+64200800 None:intergenic
GATCTGACCTGGACTCTGGT+AGG 0.558734 1.4:+64200333 None:intergenic
AGGCTCTCACTTGTTTCCAA+AGG 0.561908 1.4:-64200746 MS.gene005035:CDS
ACGATAGAGGCTTTGCCGGA+GGG 0.562687 1.4:-64200815 MS.gene005035:CDS
GGATTTCTGGAGATTGAGAT+GGG 0.566350 1.4:-64199937 MS.gene005035:CDS
ATAAGATCTGACCTGGACTC+TGG 0.567399 1.4:+64200329 None:intergenic
AAACAGTGAGGACGCAGACT+GGG 0.567855 1.4:+64200646 None:intergenic
TGGATTTCTGGAGATTGAGA+TGG 0.574304 1.4:-64199938 MS.gene005035:CDS
GGATAACCCTACCAGAGTCC+AGG 0.575507 1.4:-64200340 MS.gene005035:intron
AGAAGTTGCTAGACTTCGTA+CGG 0.575916 1.4:-64200211 MS.gene005035:CDS
AAATAAGACCTAAGAAGTGA+AGG 0.584107 1.4:-64199813 None:intergenic
AACATTCTTGGTAGATACAC+CGG 0.584303 1.4:+64200038 None:intergenic
AGAAGATGAAGTTGTTGAGA+TGG 0.589182 1.4:-64199893 MS.gene005035:CDS
GAACGAAATGACGAGTTCCA+AGG 0.592057 1.4:-64199988 MS.gene005035:CDS
CAACTCCAGTCGATTCATGT+AGG 0.592468 1.4:-64200766 MS.gene005035:CDS
CACGATAGAGGCTTTGCCGG+AGG 0.594118 1.4:-64200816 MS.gene005035:CDS
AGACTTCTTGGAGGGAGAGG+TGG 0.596099 1.4:+64200622 None:intergenic
GAGAGCCTACATGAATCGAC+TGG 0.597497 1.4:+64200761 None:intergenic
GAAGATGAAGTTGTTGAGAT+GGG 0.599122 1.4:-64199892 MS.gene005035:CDS
AGGAAGATCTTCAAAACGAT+AGG 0.608932 1.4:+64200095 None:intergenic
TCTTGGTAGATACACCGGCT+TGG 0.611482 1.4:+64200043 None:intergenic
ACACCCACAGTTAACTTGGT+AGG 0.615136 1.4:+64200075 None:intergenic
AGATATAGAGACTTCTTGGA+GGG 0.617472 1.4:+64200614 None:intergenic
TCTTCCTACCAAGTTAACTG+TGG 0.632245 1.4:-64200079 MS.gene005035:CDS
CTTCCTACCAAGTTAACTGT+GGG 0.632517 1.4:-64200078 MS.gene005035:CDS
AGAGATCTCTCCATTACTTG+GGG 0.633491 1.4:-64200386 MS.gene005035:CDS
GGTGTACTTGGAGACCAAGC+CGG 0.634271 1.4:-64200057 MS.gene005035:CDS
GTTATCCAATCCCAATAGCA+AGG 0.634767 1.4:+64200356 None:intergenic
CCAAACAATATCTGATTCAG+CGG 0.635686 1.4:+64200712 None:intergenic
ATAAAGGGTGGTCAAAACAA+AGG 0.642537 1.4:-64199859 MS.gene005035:CDS
TGATTGAGATCAAATCGGAT+GGG 0.645104 1.4:+64200678 None:intergenic
GATCCCATCATAATCAACAA+TGG 0.651324 1.4:-64200578 MS.gene005035:intron
AAGGGTGGTCAAAACAAAGG+TGG 0.659675 1.4:-64199856 MS.gene005035:CDS
GGGAGAGGTGGTAAACAGTG+AGG 0.699879 1.4:+64200634 None:intergenic
GGTGTACTTGAAATAAAGGG+TGG 0.703552 1.4:-64199871 MS.gene005035:CDS
TTTCAATTAGAGAAACGGAG+TGG 0.709938 1.4:-64200434 MS.gene005035:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAACTTGACTCTTTATTA+TGG - chr1.4:64200173-64200192 MS.gene005035:CDS 20.0%
! AGAAATGATTGAGATCAAAT+CGG + chr1.4:64199979-64199998 None:intergenic 25.0%
! CATAATCAACAATGGTAAAA+AGG - chr1.4:64200079-64200098 MS.gene005035:CDS 25.0%
!! TTACCATTGTTGATTATGAT+GGG + chr1.4:64200077-64200096 None:intergenic 25.0%
!! TTTACCATTGTTGATTATGA+TGG + chr1.4:64200078-64200097 None:intergenic 25.0%
AATCTAAATAAGATCTGACC+TGG + chr1.4:64200330-64200349 None:intergenic 30.0%
AGAGCTTTCAATTAGAGAAA+CGG - chr1.4:64200210-64200229 MS.gene005035:CDS 30.0%
ATGGGTGTACTTGAAATAAA+GGG - chr1.4:64200775-64200794 MS.gene005035:CDS 30.0%
CAAAAATCGACAACTTGATT+TGG + chr1.4:64200123-64200142 None:intergenic 30.0%
!! CATTTACTGCTGATTGATTT+TGG + chr1.4:64200388-64200407 None:intergenic 30.0%
!! TCTGCTGGTTTGAAATTTAT+GGG - chr1.4:64200461-64200480 MS.gene005035:CDS 30.0%
!! TTGAAAGCTCTGAAATTTAG+CGG + chr1.4:64200201-64200220 None:intergenic 30.0%
AAAGGTGGTTTCATTCTTGA+AGG - chr1.4:64200808-64200827 MS.gene005035:CDS 35.0%
AACATTCTTGGTAGATACAC+CGG + chr1.4:64200614-64200633 None:intergenic 35.0%
AGAAGATGAAGTTGTTGAGA+TGG - chr1.4:64200756-64200775 MS.gene005035:CDS 35.0%
AGATATAGAGACTTCTTGGA+GGG + chr1.4:64200038-64200057 None:intergenic 35.0%
AGCTACGTATTGAGTATTTG+AGG + chr1.4:64200509-64200528 None:intergenic 35.0%
AGGAAGATCTTCAAAACGAT+AGG + chr1.4:64200557-64200576 None:intergenic 35.0%
AGTGAGATATAGAGACTTCT+TGG + chr1.4:64200042-64200061 None:intergenic 35.0%
AGTTGTATGCGACAATATCT+TGG + chr1.4:64199868-64199887 None:intergenic 35.0%
ATAAAGGGTGGTCAAAACAA+AGG - chr1.4:64200790-64200809 MS.gene005035:CDS 35.0%
ATCTACCAAGAATGTTTGGT+TGG - chr1.4:64200618-64200637 MS.gene005035:CDS 35.0%
ATGATTGAGATCAAATCGGA+TGG + chr1.4:64199975-64199994 None:intergenic 35.0%
CCAAACAATATCTGATTCAG+CGG + chr1.4:64199940-64199959 None:intergenic 35.0%
CGCTGAATCAGATATTGTTT+GGG - chr1.4:64199938-64199957 MS.gene005035:CDS 35.0%
GAAGATGAAGTTGTTGAGAT+GGG - chr1.4:64200757-64200776 MS.gene005035:CDS 35.0%
GATCCCATCATAATCAACAA+TGG - chr1.4:64200071-64200090 MS.gene005035:CDS 35.0%
GATGGGTGTACTTGAAATAA+AGG - chr1.4:64200774-64200793 MS.gene005035:CDS 35.0%
GTGTATCTACCAAGAATGTT+TGG - chr1.4:64200614-64200633 MS.gene005035:CDS 35.0%
GTTCTCTATCATCATAGTCT+GGG + chr1.4:64200645-64200664 None:intergenic 35.0%
TAGAGATCTCTCCATTACTT+GGG - chr1.4:64200262-64200281 MS.gene005035:intron 35.0%
TCTCAACAACTTCATCTTCT+AGG + chr1.4:64200756-64200775 None:intergenic 35.0%
TGATTGAGATCAAATCGGAT+GGG + chr1.4:64199974-64199993 None:intergenic 35.0%
TTTCAATTAGAGAAACGGAG+TGG - chr1.4:64200215-64200234 MS.gene005035:CDS 35.0%
! AATCCATCACTTCTCACTTT+TGG + chr1.4:64200697-64200716 None:intergenic 35.0%
! AGAAGAAAAAAGCACGATAG+AGG - chr1.4:64199821-64199840 MS.gene005035:CDS 35.0%
!! GTCTGCTGGTTTGAAATTTA+TGG - chr1.4:64200460-64200479 MS.gene005035:CDS 35.0%
!! TACTGCTGATTGATTTTGGT+AGG + chr1.4:64200384-64200403 None:intergenic 35.0%
AAGTACACCCACAGTTAACT+TGG + chr1.4:64200581-64200600 None:intergenic 40.0%
AAGTTAACTGTGGGTGTACT+TGG - chr1.4:64200580-64200599 MS.gene005035:CDS 40.0%
AGAAGTTGCTAGACTTCGTA+CGG - chr1.4:64200438-64200457 MS.gene005035:CDS 40.0%
AGAGAAACGGAGTGGTAAAA+AGG - chr1.4:64200223-64200242 MS.gene005035:intron 40.0%
AGAGATCTCTCCATTACTTG+GGG - chr1.4:64200263-64200282 MS.gene005035:intron 40.0%
ATGGGAGAGTTCTTCAATTC+AGG - chr1.4:64200730-64200749 MS.gene005035:CDS 40.0%
CCGCTGAATCAGATATTGTT+TGG - chr1.4:64199937-64199956 MS.gene005035:CDS 40.0%
CGTTCTCTATCATCATAGTC+TGG + chr1.4:64200646-64200665 None:intergenic 40.0%
CTAGAGATCTCTCCATTACT+TGG - chr1.4:64200261-64200280 MS.gene005035:intron 40.0%
CTTCCTACCAAGTTAACTGT+GGG - chr1.4:64200571-64200590 MS.gene005035:intron 40.0%
GAGATATAGAGACTTCTTGG+AGG + chr1.4:64200039-64200058 None:intergenic 40.0%
GGTGTACTTGAAATAAAGGG+TGG - chr1.4:64200778-64200797 MS.gene005035:CDS 40.0%
GTTATCCAATCCCAATAGCA+AGG + chr1.4:64200296-64200315 None:intergenic 40.0%
TACGAAGTCTAGCAACTTCT+TGG + chr1.4:64200438-64200457 None:intergenic 40.0%
TCTTCCTACCAAGTTAACTG+TGG - chr1.4:64200570-64200589 MS.gene005035:intron 40.0%
! GGATTTCTGGAGATTGAGAT+GGG - chr1.4:64200712-64200731 MS.gene005035:CDS 40.0%
! TGGATTTCTGGAGATTGAGA+TGG - chr1.4:64200711-64200730 MS.gene005035:CDS 40.0%
!! AGTGAGAAGTGATGGATTTC+TGG - chr1.4:64200699-64200718 MS.gene005035:CDS 40.0%
AAGGGTGGTCAAAACAAAGG+TGG - chr1.4:64200793-64200812 MS.gene005035:CDS 45.0%
AGGCTCTCACTTGTTTCCAA+AGG - chr1.4:64199903-64199922 MS.gene005035:CDS 45.0%
ATAAGATCTGACCTGGACTC+TGG + chr1.4:64200323-64200342 None:intergenic 45.0%
CGTCCAAAAGTGAGAAGTGA+TGG - chr1.4:64200691-64200710 MS.gene005035:CDS 45.0%
GAACGAAATGACGAGTTCCA+AGG - chr1.4:64200661-64200680 MS.gene005035:CDS 45.0%
TGGGTCCAACCAAACATTCT+TGG + chr1.4:64200626-64200645 None:intergenic 45.0%
! ACACCCACAGTTAACTTGGT+AGG + chr1.4:64200577-64200596 None:intergenic 45.0%
! CAACTCCAGTCGATTCATGT+AGG - chr1.4:64199883-64199902 MS.gene005035:CDS 45.0%
! CTTTTGGACGTTCTAGTCCT+TGG + chr1.4:64200681-64200700 None:intergenic 45.0%
!! ACACTCCTTGCTATTGGGAT+TGG - chr1.4:64200288-64200307 MS.gene005035:intron 45.0%
AAACAGTGAGGACGCAGACT+GGG + chr1.4:64200006-64200025 None:intergenic 50.0%
AAGCACGATAGAGGCTTTGC+CGG - chr1.4:64199830-64199849 MS.gene005035:CDS 50.0%
AGCGGCAGAACAAAAGCCTT+TGG + chr1.4:64199922-64199941 None:intergenic 50.0%
ATCTGACCTGGACTCTGGTA+GGG + chr1.4:64200318-64200337 None:intergenic 50.0%
ATCTTGGCAATGCATCCCTC+CGG + chr1.4:64199852-64199871 None:intergenic 50.0%
GAGAGCCTACATGAATCGAC+TGG + chr1.4:64199891-64199910 None:intergenic 50.0%
TAAACAGTGAGGACGCAGAC+TGG + chr1.4:64200007-64200026 None:intergenic 50.0%
TAGAGACTTCTTGGAGGGAG+AGG + chr1.4:64200033-64200052 None:intergenic 50.0%
! TCTTGGTAGATACACCGGCT+TGG + chr1.4:64200609-64200628 None:intergenic 50.0%
!! AGGAGTGTCACCCCAAGTAA+TGG + chr1.4:64200276-64200295 None:intergenic 50.0%
!! GGGTGACACTCCTTGCTATT+GGG - chr1.4:64200283-64200302 MS.gene005035:intron 50.0%
ACGATAGAGGCTTTGCCGGA+GGG - chr1.4:64199834-64199853 MS.gene005035:CDS 55.0%
AGACTTCTTGGAGGGAGAGG+TGG + chr1.4:64200030-64200049 None:intergenic 55.0%
CTAGACTTCGTACGGTCTGC+TGG - chr1.4:64200446-64200465 MS.gene005035:CDS 55.0%
GATCTGACCTGGACTCTGGT+AGG + chr1.4:64200319-64200338 None:intergenic 55.0%
GGATAACCCTACCAGAGTCC+AGG - chr1.4:64200309-64200328 MS.gene005035:intron 55.0%
GGGAGAGGTGGTAAACAGTG+AGG + chr1.4:64200018-64200037 None:intergenic 55.0%
GGTGTACTTGGAGACCAAGC+CGG - chr1.4:64200592-64200611 MS.gene005035:CDS 55.0%
! GGGGTGACACTCCTTGCTAT+TGG - chr1.4:64200282-64200301 MS.gene005035:intron 55.0%
CACGATAGAGGCTTTGCCGG+AGG - chr1.4:64199833-64199852 MS.gene005035:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 64199816 64200855 64199816 ID=MS.gene005035
chr1.4 mRNA 64199816 64200855 64199816 ID=MS.gene005035.t1;Parent=MS.gene005035
chr1.4 exon 64200579 64200855 64200579 ID=MS.gene005035.t1.exon1;Parent=MS.gene005035.t1
chr1.4 CDS 64200579 64200855 64200579 ID=cds.MS.gene005035.t1;Parent=MS.gene005035.t1
chr1.4 exon 64200341 64200506 64200341 ID=MS.gene005035.t1.exon2;Parent=MS.gene005035.t1
chr1.4 CDS 64200341 64200506 64200341 ID=cds.MS.gene005035.t1;Parent=MS.gene005035.t1
chr1.4 exon 64199816 64200239 64199816 ID=MS.gene005035.t1.exon3;Parent=MS.gene005035.t1
chr1.4 CDS 64199816 64200239 64199816 ID=cds.MS.gene005035.t1;Parent=MS.gene005035.t1
Gene Sequence

>MS.gene005035

ATGGAAGAAGAAAAAAGCACGATAGAGGCTTTGCCGGAGGGATGCATTGCCAAGATATTGTCGCATACAACTCCAGTCGATTCATGTAGGCTCTCACTTGTTTCCAAAGGCTTTTGTTCTGCCGCTGAATCAGATATTGTTTGGGATCGTTTTCTCCCATCCGATTTGATCTCAATCATTTCTGATTCCCAGTCTGCGTCCTCACTGTTTACCACCTCTCCCTCCAAGAAGTCTCTATATCTCACTCTCTCTGACGATCCCATCATAATCAACAATGGTAAAAAGGTATCATTCTCACAAACTACCAAATCAAGTTGTCGATTTTTGCTATAATAATTATTCATTATAGTTAGAATGTTAAACTTGACTCTTTATTATGGTGCCGCTAAATTTCAGAGCTTTCAATTAGAGAAACGGAGTGGTAAAAAGGTATACATGCTTAGTGCTAGAGATCTCTCCATTACTTGGGGTGACACTCCTTGCTATTGGGATTGGATAACCCTACCAGAGTCCAGGTCAGATCTTATTTAGATTTTCTTTACTAGTAGTAATTGATTTTCTCAATCCTACCAAAATCAATCAGCAGTAAATGATTTTGTTCACAACTTTTGAACAGATTCCAAGAAGTTGCTAGACTTCGTACGGTCTGCTGGTTTGAAATTTATGGGCATATTAATAAACGTGTTTTATCCTCAAATACTCAATACGTAGCTTTTCTCGTGTTTAAGATGATCAATGCCTATCGTTTTGAAGATCTTCCTACCAAGTTAACTGTGGGTGTACTTGGAGACCAAGCCGGTGTATCTACCAAGAATGTTTGGTTGGACCCAGACTATGATGATAGAGAACGAAATGACGAGTTCCAAGGACTAGAACGTCCAAAAGTGAGAAGTGATGGATTTCTGGAGATTGAGATGGGAGAGTTCTTCAATTCAGGCCTAGAAGATGAAGTTGTTGAGATGGGTGTACTTGAAATAAAGGGTGGTCAAAACAAAGGTGGTTTCATTCTTGAAGGAATAGAAATAAGACCTAAGAAGTGA
Protein sequence

>MS.gene005035.t1

MEEEKSTIEALPEGCIAKILSHTTPVDSCRLSLVSKGFCSAAESDIVWDRFLPSDLISIISDSQSASSLFTTSPSKKSLYLTLSDDPIIINNVRMLNLTLYYGAAKFQSFQLEKRSGKKVYMLSARDLSITWGDTPCYWDWITLPESRFQEVARLRTVCWFEIYGHINKRVLSSNTQYVAFLVFKMINAYRFEDLPTKLTVGVLGDQAGVSTKNVWLDPDYDDRERNDEFQGLERPKVRSDGFLEIEMGEFFNSGLEDEVVEMGVLEIKGGQNKGGFILEGIEIRPKK