AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74873

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74873.t1 MTR_1g079370 94.007 267 14 1 1 267 1 265 0.0 508
MS.gene74873.t1 MTR_1g079180 78.889 270 49 4 1 267 1 265 2.91e-155 433
MS.gene74873.t1 MTR_1g079380 84.270 267 40 1 1 267 1 265 1.40e-153 430
MS.gene74873.t1 MTR_1g100597 71.533 274 69 3 1 267 1 272 4.45e-139 392
MS.gene74873.t1 MTR_1g079260 69.485 272 78 2 1 267 1 272 1.44e-138 391
MS.gene74873.t1 MTR_1g079200 72.862 269 66 3 1 267 1 264 3.32e-138 390
MS.gene74873.t1 MTR_1g079460 70.000 270 74 2 1 267 1 266 1.18e-135 384
MS.gene74873.t1 MTR_1g079230 65.343 277 82 3 1 267 1 273 1.46e-124 355
MS.gene74873.t1 MTR_1g079850 64.057 281 87 3 1 267 1 281 1.63e-120 345
MS.gene74873.t1 MTR_1g100613 69.796 245 64 3 15 250 1 244 4.85e-118 338
MS.gene74873.t1 MTR_1g079250 63.669 278 90 5 1 267 1 278 9.19e-115 332
MS.gene74873.t1 MTR_1g026510 59.364 283 99 6 1 267 1 283 2.64e-112 325
MS.gene74873.t1 MTR_3g452600 62.121 264 94 2 4 267 10 267 3.70e-111 322
MS.gene74873.t1 MTR_3g006850 59.197 299 87 5 4 267 17 315 6.23e-110 320
MS.gene74873.t1 MTR_2g079100 60.076 263 93 3 4 264 9 261 8.86e-101 295
MS.gene74873.t1 MTR_3g450990 56.071 280 101 4 4 267 10 283 2.67e-98 289
MS.gene74873.t1 MTR_1g079410 55.556 270 110 4 4 267 8 273 1.11e-97 287
MS.gene74873.t1 MTR_1g079350 57.554 278 104 6 1 267 1 275 4.11e-97 286
MS.gene74873.t1 MTR_1g079140 48.986 296 119 5 3 267 12 306 2.40e-91 273
MS.gene74873.t1 MTR_1g079340 52.740 292 107 7 1 267 40 325 4.75e-87 262
MS.gene74873.t1 MTR_1g078470 73.143 175 40 3 93 267 17 184 6.99e-87 256
MS.gene74873.t1 MTR_7g096300 57.708 253 90 5 1 247 1 242 1.97e-85 270
MS.gene74873.t1 MTR_1g079400 48.421 285 109 4 4 267 12 279 1.28e-80 244
MS.gene74873.t1 MTR_7g112550 45.390 282 135 8 2 267 5 283 8.80e-71 219
MS.gene74873.t1 MTR_1g083580 39.928 278 153 6 2 267 6 281 2.37e-65 205
MS.gene74873.t1 MTR_1g079240 59.563 183 61 4 85 267 10 179 1.23e-62 195
MS.gene74873.t1 MTR_7g112560 40.152 264 145 6 7 267 4 257 2.66e-61 194
MS.gene74873.t1 MTR_1g076870 62.687 134 36 1 128 247 2 135 3.67e-51 164
MS.gene74873.t1 MTR_4g023570 37.500 264 132 7 7 267 4 237 6.51e-51 167
MS.gene74873.t1 MTR_1g079350 53.293 167 67 4 1 159 1 164 6.34e-48 159
MS.gene74873.t1 MTR_1g079130 44.974 189 82 3 101 267 1 189 5.55e-47 155
MS.gene74873.t1 MTR_1g079210 57.724 123 42 1 129 241 7 129 9.16e-40 135
MS.gene74873.t1 MTR_7g096310 53.333 150 62 3 38 185 19 162 1.93e-39 135
MS.gene74873.t1 MTR_2g042540 64.762 105 35 2 1 103 1 105 1.12e-37 129
MS.gene74873.t1 MTR_1g078460 73.563 87 23 0 1 87 64 150 4.22e-37 129
MS.gene74873.t1 MTR_3g058640 59.615 104 38 1 128 231 7 106 3.74e-34 119
MS.gene74873.t1 MTR_7g103380 26.978 278 175 6 4 266 19 283 2.10e-29 112
MS.gene74873.t1 MTR_1g079220 74.286 70 14 1 165 234 1 66 1.27e-27 102
MS.gene74873.t1 MTR_4g083970 28.571 210 124 4 63 267 102 290 8.62e-24 97.8
MS.gene74873.t1 MTR_0113s0040 27.465 284 161 6 2 267 20 276 1.14e-22 94.7
MS.gene74873.t1 MTR_1g101390 27.305 282 162 10 2 266 26 281 1.36e-20 89.0
MS.gene74873.t1 MTR_0002s0020 26.374 273 175 7 3 264 21 278 2.58e-19 85.5
MS.gene74873.t1 MTR_4g102770 34.247 146 92 2 4 146 19 163 5.40e-19 84.7
MS.gene74873.t1 MTR_5g010800 26.545 275 173 9 2 266 15 270 1.27e-18 83.6
MS.gene74873.t1 MTR_1g079390 76.000 50 10 2 213 262 8 55 1.12e-17 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74873.t1 AT2G02360 48.669 263 121 5 7 266 17 268 3.59e-85 256
MS.gene74873.t1 AT1G80110 46.970 264 130 5 4 266 1 255 2.85e-72 222
MS.gene74873.t1 AT2G02240 44.656 262 134 5 7 266 64 316 1.79e-71 222
MS.gene74873.t1 AT2G02340 43.750 272 144 5 1 266 33 301 2.08e-71 221
MS.gene74873.t1 AT2G02250 43.820 267 136 7 4 266 47 303 3.46e-69 216
MS.gene74873.t1 AT1G09155 43.214 280 138 8 7 267 3 280 1.66e-67 211
MS.gene74873.t1 AT2G02230 38.079 302 143 6 7 267 35 333 5.75e-66 209
MS.gene74873.t1 AT1G56250 43.011 279 140 6 7 267 4 281 5.76e-62 197
MS.gene74873.t1 AT1G56240 42.446 278 141 6 7 266 4 280 9.72e-62 196
MS.gene74873.t1 AT2G02350 40.075 267 148 6 4 266 24 282 2.82e-58 187
MS.gene74873.t1 AT2G02320 40.299 268 142 7 6 266 42 298 1.20e-54 179
MS.gene74873.t1 AT2G02310 35.581 267 162 4 4 267 45 304 4.32e-53 175
MS.gene74873.t1 AT2G02300 37.500 272 138 8 4 267 34 281 8.25e-49 163
MS.gene74873.t1 AT5G24560 42.105 190 97 4 6 192 5 184 1.75e-43 146
MS.gene74873.t1 AT3G53000 28.214 280 168 8 4 266 21 284 3.68e-34 125
MS.gene74873.t1 AT2G02350 38.235 170 96 5 101 266 1 165 4.10e-29 108
MS.gene74873.t1 AT3G61060 27.372 274 167 9 6 267 27 280 6.78e-22 92.8
MS.gene74873.t1 AT1G33920 31.461 178 94 4 96 267 9 164 1.19e-21 89.0
MS.gene74873.t1 AT3G61060 26.642 274 170 8 6 267 27 281 2.17e-21 91.3
MS.gene74873.t1 AT4G19850 34.409 186 84 8 94 267 59 218 3.26e-20 86.7
MS.gene74873.t1 AT4G19850 34.409 186 84 8 94 267 17 176 3.43e-20 85.5
MS.gene74873.t1 AT1G12710 25.357 280 166 6 4 266 28 281 1.67e-19 86.3
MS.gene74873.t1 AT1G63090 28.571 203 123 5 4 189 26 223 9.26e-19 84.0
MS.gene74873.t1 AT4G19840 30.814 172 96 5 99 267 94 245 3.99e-17 79.0
MS.gene74873.t1 AT5G52120 26.460 291 161 9 1 266 18 280 8.91e-17 78.6
MS.gene74873.t1 AT1G12710 33.582 134 83 3 4 134 28 158 1.40e-14 72.8
MS.gene74873.t1 AT4G19850 37.500 120 62 6 94 202 59 176 1.15e-12 65.9
MS.gene74873.t1 AT2G26820 27.119 177 103 5 97 266 320 477 7.27e-11 62.4

Find 69 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGCTGCATATTGAGTATT+TGG 0.137691 1.1:+58960484 None:intergenic
CTAGATCTCTTGCAATTGTT+TGG 0.246175 1.1:-58962008 MS.gene74873:CDS
TAGATCTCTTGCAATTGTTT+GGG 0.285923 1.1:-58962007 MS.gene74873:CDS
TGGAGATAAAGGCTCTTAAT+TGG 0.287168 1.1:-58960204 MS.gene74873:CDS
TCCACTTCCACTTCCACATT+TGG 0.287872 1.1:+58960355 None:intergenic
GATGAGTTTGTTGGAGATAA+AGG 0.307324 1.1:-58960215 MS.gene74873:CDS
TTCTTGAAGGAATAGAAATT+AGG 0.317502 1.1:-58960168 MS.gene74873:CDS
TGCCAACGCTCCCACCAAAA+AGG 0.334601 1.1:-58962248 MS.gene74873:CDS
AAGCTGCATATTGAGTATTT+GGG 0.334659 1.1:+58960485 None:intergenic
GGATTCCTTTGTCTGATTCC+AGG 0.334679 1.1:-58961960 MS.gene74873:intron
TCAACAAGCTTAGCAACTTC+AGG 0.357954 1.1:+58960556 None:intergenic
TGATCATGATCGATGATGAT+AGG 0.375392 1.1:+58962199 None:intergenic
GTTCAAAATGATTGATGCTT+GGG 0.383807 1.1:-58960455 MS.gene74873:CDS
ATTCCATACGGCATCGGAAT+CGG 0.408752 1.1:+58962321 None:intergenic
GCTGCTTAATTGTGTTACTT+AGG 0.411610 1.1:+58960145 None:intergenic
TAGTACTAAAATTGTCTGTT+TGG 0.420764 1.1:-58960380 MS.gene74873:CDS
GACAGAGATTCAGGATTTGC+CGG 0.423087 1.1:-58962431 MS.gene74873:CDS
AAGAGTGGTCTCTTTCTTGA+AGG 0.428845 1.1:-58960181 MS.gene74873:CDS
ATACTAAGCACTATTGGAAC+TGG 0.440686 1.1:-58961981 MS.gene74873:CDS
TGGAGTTGTACGAGAGACTA+TGG 0.442874 1.1:+58962393 None:intergenic
GGGTGGTTGGAGATTGAGAT+GGG 0.458032 1.1:-58960277 MS.gene74873:CDS
GGGGTGATACTAAGCACTAT+TGG 0.458105 1.1:-58961987 MS.gene74873:CDS
CTAAGCTTGTTGACGTATGT+TGG 0.459650 1.1:-58960546 MS.gene74873:CDS
CATCCGATTCCGATGCCGTA+TGG 0.476407 1.1:-58962324 MS.gene74873:CDS
CATCATCGATCATGATCAAA+AGG 0.478451 1.1:-58962194 MS.gene74873:intron
AGAAGTCCTGATGAGTTTGT+TGG 0.481141 1.1:-58960224 MS.gene74873:CDS
ATGGCGGCAATGCATCCTTC+CGG 0.482859 1.1:+58962412 None:intergenic
CGTCCTAGTGTGAGAAGCGA+TGG 0.485861 1.1:-58960298 MS.gene74873:CDS
TTATCTCCAACAAACTCATC+AGG 0.495784 1.1:+58960218 None:intergenic
TCCAAATGTGGAAGTGGAAG+TGG 0.508630 1.1:-58960356 MS.gene74873:CDS
TGTTCAAAATGATTGATGCT+TGG 0.510985 1.1:-58960456 MS.gene74873:CDS
TTTCAATTGGACAGAAAGAG+TGG 0.517102 1.1:-58962054 MS.gene74873:CDS
TGGGTGGTTGGAGATTGAGA+TGG 0.517146 1.1:-58960278 MS.gene74873:CDS
GGATGCAGATCGGAAAGTCT+TGG 0.523752 1.1:+58962342 None:intergenic
CAACAAGCTTAGCAACTTCA+GGG 0.526604 1.1:+58960557 None:intergenic
GAGAGTCTGCCGGCGTCGAG+TGG 0.527104 1.1:+58962373 None:intergenic
GTGGGAGCGTTGGCAAGGGA+AGG 0.528441 1.1:+58962256 None:intergenic
ATGCAGCATAACAGAGCAGT+AGG 0.534871 1.1:-58960328 MS.gene74873:CDS
CACCCATCGCTTCTCACACT+AGG 0.543006 1.1:+58960295 None:intergenic
ATGATAGATGAGATGTCAGA+AGG 0.548763 1.1:+58962289 None:intergenic
AGATCTCTTGCAATTGTTTG+GGG 0.552612 1.1:-58962006 MS.gene74873:CDS
GAGATTCAGGATTTGCCGGA+AGG 0.560627 1.1:-58962427 MS.gene74873:CDS
GGGATTTCAGAACTGTCCTG+TGG 0.565853 1.1:-58960434 MS.gene74873:CDS
TTTGGATCCAAATGTGGAAG+TGG 0.569537 1.1:-58960362 MS.gene74873:CDS
GGAAACGACGGAGAGTCTGC+CGG 0.570360 1.1:+58962363 None:intergenic
AAGGCTCTTAATTGGAAGAG+TGG 0.575926 1.1:-58960196 MS.gene74873:CDS
TGTGAGAAGCGATGGGTGGT+TGG 0.576323 1.1:-58960290 MS.gene74873:CDS
AGGTAGAAATTGATTCCATA+CGG 0.579202 1.1:+58962309 None:intergenic
AAATTGATTCCATACGGCAT+CGG 0.587612 1.1:+58962315 None:intergenic
CATCGGAATCGGATGCAGAT+CGG 0.588216 1.1:+58962332 None:intergenic
GTATGTTGGCTTGAAATTCG+TGG 0.591694 1.1:-58960532 MS.gene74873:CDS
TTTGGTGGGAGCGTTGGCAA+GGG 0.595797 1.1:+58962252 None:intergenic
TTCAAAATGATTGATGCTTG+GGG 0.600421 1.1:-58960454 MS.gene74873:CDS
AGCTGCATATTGAGTATTTG+GGG 0.601395 1.1:+58960486 None:intergenic
TTATCAGTTGGTGTCGAAGG+TGG 0.605148 1.1:-58960409 MS.gene74873:CDS
ACACCAACTGATAACTCCAC+AGG 0.607721 1.1:+58960418 None:intergenic
GTGGTTGGAGATTGAGATGG+GGG 0.617398 1.1:-58960275 MS.gene74873:CDS
GAGTTATCAGTTGGTGTCGA+AGG 0.620810 1.1:-58960412 MS.gene74873:CDS
AGTATTTGGGGACAGAACAA+TGG 0.623021 1.1:+58960498 None:intergenic
TGTCCTGTGGAGTTATCAGT+TGG 0.623846 1.1:-58960421 MS.gene74873:CDS
TGTCTGTTTGGATCCAAATG+TGG 0.625217 1.1:-58960368 MS.gene74873:CDS
GGTGGTTGGAGATTGAGATG+GGG 0.640148 1.1:-58960276 MS.gene74873:CDS
GTCCTAGTGTGAGAAGCGAT+GGG 0.643186 1.1:-58960297 MS.gene74873:CDS
ACTAACCTGGAATCAGACAA+AGG 0.649145 1.1:+58961955 None:intergenic
TCGGAAAGTCTTGGAAACGA+CGG 0.681316 1.1:+58962351 None:intergenic
CTAGTGTGAGAAGCGATGGG+TGG 0.709944 1.1:-58960294 MS.gene74873:CDS
CGTACAACTCCACTCGACGC+CGG 0.717247 1.1:-58962382 MS.gene74873:CDS
AATGTGGAAGTGGAAGTGGA+AGG 0.722814 1.1:-58960352 MS.gene74873:CDS
AGTTGTACGAGAGACTATGG+CGG 0.789727 1.1:+58962396 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATAAA+AGG + chr1.1:58961848-58961867 None:intergenic 0.0%
!! ATTTCTCGAATATAATATTA+AGG - chr1.1:58960445-58960464 MS.gene74873:CDS 15.0%
!! TATTATATTCGAGAAATTGA+CGG + chr1.1:58960442-58960461 None:intergenic 20.0%
!! TTGTAAAGTAACAAACAAAT+TGG + chr1.1:58961347-58961366 None:intergenic 20.0%
!!! AAATTTTCAGAGTTTTCAAT+TGG - chr1.1:58960525-58960544 MS.gene74873:CDS 20.0%
!!! ATTGAACATGCTTTAAATTT+GGG + chr1.1:58961046-58961065 None:intergenic 20.0%
!!! CTTTGTTTGATAAAAAATAG+TGG + chr1.1:58960783-58960802 None:intergenic 20.0%
!!! GTAAAATTTCTAAGAAAACA+TGG + chr1.1:58961492-58961511 None:intergenic 20.0%
!!! TGGTCAGATATTTTAAAATT+TGG + chr1.1:58961327-58961346 None:intergenic 20.0%
! ATACCATATATCAAAATCCA+TGG - chr1.1:58960994-58961013 MS.gene74873:intron 25.0%
! ATCATTCAAGTCCATAAAAA+AGG + chr1.1:58960812-58960831 None:intergenic 25.0%
! GATGCATTCAAAATCATATA+GGG + chr1.1:58961298-58961317 None:intergenic 25.0%
! TAGTACTAAAATTGTCTGTT+TGG - chr1.1:58962212-58962231 MS.gene74873:CDS 25.0%
! TCAAATATAACGGTTTGATT+CGG + chr1.1:58960706-58960725 None:intergenic 25.0%
! TGATGCATTCAAAATCATAT+AGG + chr1.1:58961299-58961318 None:intergenic 25.0%
! TGCTATTAGCTCAAATATAA+CGG + chr1.1:58960716-58960735 None:intergenic 25.0%
! TTCTTGAAGGAATAGAAATT+AGG - chr1.1:58962424-58962443 MS.gene74873:CDS 25.0%
!! GATTGAACATGCTTTAAATT+TGG + chr1.1:58961047-58961066 None:intergenic 25.0%
!! TGAAAACTCTGAAAATTTGA+AGG + chr1.1:58960523-58960542 None:intergenic 25.0%
!!! ATGGATTTTGATATATGGTA+TGG + chr1.1:58960995-58961014 None:intergenic 25.0%
!!! TAAGTTATATATGCCGTTTT+AGG + chr1.1:58961721-58961740 None:intergenic 25.0%
AAGCTGCATATTGAGTATTT+GGG + chr1.1:58962110-58962129 None:intergenic 30.0%
ACATTAACTGATCACTAACA+TGG + chr1.1:58960843-58960862 None:intergenic 30.0%
AGTGATCAGTTAATGTTGAT+GGG - chr1.1:58960847-58960866 MS.gene74873:intron 30.0%
CAAGTCCATAAAAAAGGATA+TGG + chr1.1:58960806-58960825 None:intergenic 30.0%
CAGGATCGTTAACTAAAAAT+TGG + chr1.1:58961808-58961827 None:intergenic 30.0%
CATTCAAAATCATATAGGGT+CGG + chr1.1:58961294-58961313 None:intergenic 30.0%
GAATGTTGGATTTCCTAAAA+CGG - chr1.1:58961705-58961724 MS.gene74873:intron 30.0%
GATCGTTAACTAAAAATTGG+AGG + chr1.1:58961805-58961824 None:intergenic 30.0%
GTTCAAAATGATTGATGCTT+GGG - chr1.1:58962137-58962156 MS.gene74873:intron 30.0%
TAAGCTGCATATTGAGTATT+TGG + chr1.1:58962111-58962130 None:intergenic 30.0%
TAGATCTCTTGCAATTGTTT+GGG - chr1.1:58960585-58960604 MS.gene74873:intron 30.0%
TAGTGATCAGTTAATGTTGA+TGG - chr1.1:58960846-58960865 MS.gene74873:intron 30.0%
TGTTCAAAATGATTGATGCT+TGG - chr1.1:58962136-58962155 MS.gene74873:intron 30.0%
TTCAAAATGATTGATGCTTG+GGG - chr1.1:58962138-58962157 MS.gene74873:intron 30.0%
! AATAGTTGGTGTAGTGTTAT+AGG - chr1.1:58960969-58960988 MS.gene74873:intron 30.0%
! AGGTAGAAATTGATTCCATA+CGG + chr1.1:58960286-58960305 None:intergenic 30.0%
! TCCTTTTAGGCTTTTAGTAT+GGG - chr1.1:58961675-58961694 MS.gene74873:intron 30.0%
!! AGACAATTTTAGTACTACTC+TGG + chr1.1:58962209-58962228 None:intergenic 30.0%
!! TAGCCATGGATTTTGATATA+TGG + chr1.1:58961000-58961019 None:intergenic 30.0%
!! TTTGTGTACTACTTTTGAAC+AGG - chr1.1:58962010-58962029 MS.gene74873:CDS 30.0%
!!! CAAAGCCATATCCTTTTTTA+TGG - chr1.1:58960798-58960817 MS.gene74873:intron 30.0%
!!! TTTTTTGATCCTAATTGCTG+CGG - chr1.1:58961265-58961284 MS.gene74873:intron 30.0%
ACCTTACGCAAAAGATTGAA+TGG + chr1.1:58961548-58961567 None:intergenic 35.0%
AGATCTCTTGCAATTGTTTG+GGG - chr1.1:58960586-58960605 MS.gene74873:intron 35.0%
AGCTGCATATTGAGTATTTG+GGG + chr1.1:58962109-58962128 None:intergenic 35.0%
ATACTAAGCACTATTGGAAC+TGG - chr1.1:58960611-58960630 MS.gene74873:intron 35.0%
ATGATAGATGAGATGTCAGA+AGG + chr1.1:58960306-58960325 None:intergenic 35.0%
CAGTTAATTCTTAGCAGATG+AGG + chr1.1:58960916-58960935 None:intergenic 35.0%
CATCATCGATCATGATCAAA+AGG - chr1.1:58960398-58960417 MS.gene74873:CDS 35.0%
CCAACTATTCTTCACTAACT+TGG + chr1.1:58960958-58960977 None:intergenic 35.0%
CCAAGTTAGTGAAGAATAGT+TGG - chr1.1:58960955-58960974 MS.gene74873:intron 35.0%
CTAGATCTCTTGCAATTGTT+TGG - chr1.1:58960584-58960603 MS.gene74873:intron 35.0%
GATACTTAGCTGAAGTGATA+TGG + chr1.1:58961132-58961151 None:intergenic 35.0%
GATTGTCACAGAATAAGAGT+TGG + chr1.1:58961587-58961606 None:intergenic 35.0%
GGGCATTCAAAAAGAATAAC+AGG - chr1.1:58960875-58960894 MS.gene74873:intron 35.0%
GGTCGATAAATTAACAATGC+AGG + chr1.1:58961827-58961846 None:intergenic 35.0%
TATTCGAGAAATTGACGGTT+AGG + chr1.1:58960437-58960456 None:intergenic 35.0%
TCCAAATTCTCTAAGTCACA+AGG + chr1.1:58961099-58961118 None:intergenic 35.0%
TCCCATACTAAAAGCCTAAA+AGG + chr1.1:58961679-58961698 None:intergenic 35.0%
TCCTTGTGACTTAGAGAATT+TGG - chr1.1:58961095-58961114 MS.gene74873:intron 35.0%
TGATCATGATCGATGATGAT+AGG + chr1.1:58960396-58960415 None:intergenic 35.0%
TGGAGATAAAGGCTCTTAAT+TGG - chr1.1:58962388-58962407 MS.gene74873:CDS 35.0%
TTATCTCCAACAAACTCATC+AGG + chr1.1:58962377-58962396 None:intergenic 35.0%
TTTCAATTGGACAGAAAGAG+TGG - chr1.1:58960538-58960557 MS.gene74873:CDS 35.0%
! AAATTGATTCCATACGGCAT+CGG + chr1.1:58960280-58960299 None:intergenic 35.0%
! GTCCTTTTAGGCTTTTAGTA+TGG - chr1.1:58961674-58961693 MS.gene74873:intron 35.0%
!! AGTTAATGTTGATGGGTTTC+AGG - chr1.1:58960854-58960873 MS.gene74873:intron 35.0%
!! GATGAGTTTGTTGGAGATAA+AGG - chr1.1:58962377-58962396 MS.gene74873:CDS 35.0%
!! GTGTTTTATTGCATGACAGA+TGG + chr1.1:58961949-58961968 None:intergenic 35.0%
!! GTTAATGTTGATGGGTTTCA+GGG - chr1.1:58960855-58960874 MS.gene74873:intron 35.0%
AACTGCAGAGTTACAGAAGA+AGG + chr1.1:58961223-58961242 None:intergenic 40.0%
AAGAGTGGTCTCTTTCTTGA+AGG - chr1.1:58962411-58962430 MS.gene74873:CDS 40.0%
AAGGCTCTTAATTGGAAGAG+TGG - chr1.1:58962396-58962415 MS.gene74873:CDS 40.0%
AAGTTAAGCGCTAACTAACC+TGG + chr1.1:58960653-58960672 None:intergenic 40.0%
ACTAACCTGGAATCAGACAA+AGG + chr1.1:58960640-58960659 None:intergenic 40.0%
ACTTAGCTGAAGTGATATGG+AGG + chr1.1:58961129-58961148 None:intergenic 40.0%
AGAAGTCCTGATGAGTTTGT+TGG - chr1.1:58962368-58962387 MS.gene74873:CDS 40.0%
AGTATTTGGGGACAGAACAA+TGG + chr1.1:58962097-58962116 None:intergenic 40.0%
CAACAAGCTTAGCAACTTCA+GGG + chr1.1:58962038-58962057 None:intergenic 40.0%
CACAGAATAAGAGTTGGAGT+TGG + chr1.1:58961581-58961600 None:intergenic 40.0%
CATCGATCATGATCAAAAGG+TGG - chr1.1:58960401-58960420 MS.gene74873:CDS 40.0%
CATGTAGTGCATCTTCAATG+TGG + chr1.1:58961466-58961485 None:intergenic 40.0%
GAGTTACAGAAGAAGGATCT+TGG + chr1.1:58961216-58961235 None:intergenic 40.0%
GTATGGGAGAAGATGAATGT+TGG - chr1.1:58961691-58961710 MS.gene74873:intron 40.0%
TACATAGGCCAGATAGTGTA+AGG + chr1.1:58961409-58961428 None:intergenic 40.0%
TCAACAAGCTTAGCAACTTC+AGG + chr1.1:58962039-58962058 None:intergenic 40.0%
TCCTTAGTCCTTACACTATC+TGG - chr1.1:58961398-58961417 MS.gene74873:intron 40.0%
TGTCTGTTTGGATCCAAATG+TGG - chr1.1:58962224-58962243 MS.gene74873:CDS 40.0%
TTTGGATCCAAATGTGGAAG+TGG - chr1.1:58962230-58962249 MS.gene74873:CDS 40.0%
! AGCAATGTCGATGTCCTTTT+AGG - chr1.1:58961662-58961681 MS.gene74873:intron 40.0%
! GCCATTCAATCTTTTGCGTA+AGG - chr1.1:58961544-58961563 MS.gene74873:intron 40.0%
! TAAATTTGGGTTTGAACCCG+AGG + chr1.1:58961033-58961052 None:intergenic 40.0%
!! AGATACAGAGCCTTTTTGGT+GGG + chr1.1:58960357-58960376 None:intergenic 40.0%
!! CTAAGCTTGTTGACGTATGT+TGG - chr1.1:58962046-58962065 MS.gene74873:CDS 40.0%
!! GTATGTTGGCTTGAAATTCG+TGG - chr1.1:58962060-58962079 MS.gene74873:CDS 40.0%
AATGGCCAGGGTAATTCGAT+GGG + chr1.1:58961530-58961549 None:intergenic 45.0%
AATGTGGAAGTGGAAGTGGA+AGG - chr1.1:58962240-58962259 MS.gene74873:CDS 45.0%
ACACCAACTGATAACTCCAC+AGG + chr1.1:58962177-58962196 None:intergenic 45.0%
ACACTTATCACGAGAACCTG+AGG - chr1.1:58961634-58961653 MS.gene74873:intron 45.0%
ACGCAAAAGATTGAATGGCC+AGG + chr1.1:58961543-58961562 None:intergenic 45.0%
AGTTGTACGAGAGACTATGG+CGG + chr1.1:58960199-58960218 None:intergenic 45.0%
ATGCAGCATAACAGAGCAGT+AGG - chr1.1:58962264-58962283 MS.gene74873:CDS 45.0%
ATGGGGGAATTCTTCAGTTC+AGG - chr1.1:58962333-58962352 MS.gene74873:CDS 45.0%
CGCAAAAGATTGAATGGCCA+GGG + chr1.1:58961542-58961561 None:intergenic 45.0%
GAAATCCCATCGAATTACCC+TGG - chr1.1:58961522-58961541 MS.gene74873:intron 45.0%
GACAGAGATTCAGGATTTGC+CGG - chr1.1:58960161-58960180 MS.gene74873:CDS 45.0%
GCCAGATAGTGTAAGGACTA+AGG + chr1.1:58961402-58961421 None:intergenic 45.0%
GGATTCCTTTGTCTGATTCC+AGG - chr1.1:58960632-58960651 MS.gene74873:intron 45.0%
GGCCTAGTAAGCTTCTACAT+AGG + chr1.1:58961424-58961443 None:intergenic 45.0%
GGCCTATGTAGAAGCTTACT+AGG - chr1.1:58961419-58961438 MS.gene74873:intron 45.0%
GGGGTGATACTAAGCACTAT+TGG - chr1.1:58960605-58960624 MS.gene74873:intron 45.0%
TCAGGTTCTCGTGATAAGTG+TGG + chr1.1:58961635-58961654 None:intergenic 45.0%
TCCAAATGTGGAAGTGGAAG+TGG - chr1.1:58962236-58962255 MS.gene74873:CDS 45.0%
TCCACTTCCACTTCCACATT+TGG + chr1.1:58962240-58962259 None:intergenic 45.0%
TCGGAAAGTCTTGGAAACGA+CGG + chr1.1:58960244-58960263 None:intergenic 45.0%
TGGAGTTGTACGAGAGACTA+TGG + chr1.1:58960202-58960221 None:intergenic 45.0%
TGTCCTGTGGAGTTATCAGT+TGG - chr1.1:58962171-58962190 MS.gene74873:intron 45.0%
! AGCGAGATACAGAGCCTTTT+TGG + chr1.1:58960361-58960380 None:intergenic 45.0%
! ATTCCATACGGCATCGGAAT+CGG + chr1.1:58960274-58960293 None:intergenic 45.0%
! GAGATACAGAGCCTTTTTGG+TGG + chr1.1:58960358-58960377 None:intergenic 45.0%
! TTTTGCGTCCAACTTCAGCT+TGG - chr1.1:58961186-58961205 MS.gene74873:intron 45.0%
!! GAGTTATCAGTTGGTGTCGA+AGG - chr1.1:58962180-58962199 MS.gene74873:intron 45.0%
!! TTATCAGTTGGTGTCGAAGG+TGG - chr1.1:58962183-58962202 MS.gene74873:intron 45.0%
AGCTTACTAGGCCACATGTG+TGG - chr1.1:58961431-58961450 MS.gene74873:intron 50.0%
ATGGCTAGTAGCTACCACCT+CGG - chr1.1:58961013-58961032 MS.gene74873:intron 50.0%
CATCGGAATCGGATGCAGAT+CGG + chr1.1:58960263-58960282 None:intergenic 50.0%
GAATGGCCAGGGTAATTCGA+TGG + chr1.1:58961531-58961550 None:intergenic 50.0%
GAGATTCAGGATTTGCCGGA+AGG - chr1.1:58960165-58960184 MS.gene74873:CDS 50.0%
GGATGCAGATCGGAAAGTCT+TGG + chr1.1:58960253-58960272 None:intergenic 50.0%
GGGATTTCAGAACTGTCCTG+TGG - chr1.1:58962158-58962177 MS.gene74873:intron 50.0%
GGGTGGTTGGAGATTGAGAT+GGG - chr1.1:58962315-58962334 MS.gene74873:CDS 50.0%
GGTGGTTGGAGATTGAGATG+GGG - chr1.1:58962316-58962335 MS.gene74873:CDS 50.0%
GTCCTAGTGTGAGAAGCGAT+GGG - chr1.1:58962295-58962314 MS.gene74873:CDS 50.0%
GTGGTTGGAGATTGAGATGG+GGG - chr1.1:58962317-58962336 MS.gene74873:CDS 50.0%
TCGACATTGCTATGTGCCTC+AGG + chr1.1:58961653-58961672 None:intergenic 50.0%
TGGGTGGTTGGAGATTGAGA+TGG - chr1.1:58962314-58962333 MS.gene74873:CDS 50.0%
TTGGAGCTCCAAGCTGAAGT+TGG + chr1.1:58961197-58961216 None:intergenic 50.0%
! ATTTGGGTTTGAACCCGAGG+TGG + chr1.1:58961030-58961049 None:intergenic 50.0%
ATGGCGGCAATGCATCCTTC+CGG + chr1.1:58960183-58960202 None:intergenic 55.0%
CACCCATCGCTTCTCACACT+AGG + chr1.1:58962300-58962319 None:intergenic 55.0%
CATCCGATTCCGATGCCGTA+TGG - chr1.1:58960268-58960287 MS.gene74873:CDS 55.0%
CGTCCTAGTGTGAGAAGCGA+TGG - chr1.1:58962294-58962313 MS.gene74873:CDS 55.0%
CTAGTGTGAGAAGCGATGGG+TGG - chr1.1:58962298-58962317 MS.gene74873:CDS 55.0%
GAGGTGGTAGCTACTAGCCA+TGG + chr1.1:58961014-58961033 None:intergenic 55.0%
GGATCCAGTGTCCACACATG+TGG + chr1.1:58961445-58961464 None:intergenic 55.0%
TAGGCCACATGTGTGGACAC+TGG - chr1.1:58961438-58961457 MS.gene74873:intron 55.0%
TGCCAACGCTCCCACCAAAA+AGG - chr1.1:58960344-58960363 MS.gene74873:CDS 55.0%
TGGCTAGTAGCTACCACCTC+GGG - chr1.1:58961014-58961033 MS.gene74873:intron 55.0%
TGTGAGAAGCGATGGGTGGT+TGG - chr1.1:58962302-58962321 MS.gene74873:CDS 55.0%
! GGTCGGTTTCCGCAGCAATT+AGG + chr1.1:58961277-58961296 None:intergenic 55.0%
!! AGCCTTTTTGGTGGGAGCGT+TGG + chr1.1:58960349-58960368 None:intergenic 55.0%
!! TTTGGTGGGAGCGTTGGCAA+GGG + chr1.1:58960343-58960362 None:intergenic 55.0%
!!! TTTTGGTGGGAGCGTTGGCA+AGG + chr1.1:58960344-58960363 None:intergenic 55.0%
CGTACAACTCCACTCGACGC+CGG - chr1.1:58960210-58960229 MS.gene74873:CDS 60.0%
GGAAACGACGGAGAGTCTGC+CGG + chr1.1:58960232-58960251 None:intergenic 60.0%
!! GTGGGAGCGTTGGCAAGGGA+AGG + chr1.1:58960339-58960358 None:intergenic 65.0%
! GAGAGTCTGCCGGCGTCGAG+TGG + chr1.1:58960222-58960241 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 58960159 58962455 58960159 ID=MS.gene74873
chr1.1 mRNA 58960159 58962455 58960159 ID=MS.gene74873.t1;Parent=MS.gene74873
chr1.1 exon 58962195 58962455 58962195 ID=MS.gene74873.t1.exon1;Parent=MS.gene74873.t1
chr1.1 CDS 58962195 58962455 58962195 ID=cds.MS.gene74873.t1;Parent=MS.gene74873.t1
chr1.1 exon 58961961 58962079 58961961 ID=MS.gene74873.t1.exon2;Parent=MS.gene74873.t1
chr1.1 CDS 58961961 58962079 58961961 ID=cds.MS.gene74873.t1;Parent=MS.gene74873.t1
chr1.1 exon 58960159 58960582 58960159 ID=MS.gene74873.t1.exon3;Parent=MS.gene74873.t1
chr1.1 CDS 58960159 58960582 58960159 ID=cds.MS.gene74873.t1;Parent=MS.gene74873.t1
Gene Sequence

>MS.gene74873

ATGACAGAGATTCAGGATTTGCCGGAAGGATGCATTGCCGCCATAGTCTCTCGTACAACTCCACTCGACGCCGGCAGACTCTCCGTCGTTTCCAAGACTTTCCGATCTGCATCCGATTCCGATGCCGTATGGAATCAATTTCTACCTTCTGACATCTCATCTATCATCTCACACTCTCCTTCCCTTGCCAACGCTCCCACCAAAAAGGCTCTGTATCTCGCTCTCTCTGATCGTCCTATCATCATCGATCATGATCAAAAGGTGGTATTCTTCTTCCTAACCGTCAATTTCTCGAATATAATATTAAGGCTTATAATTGAATTGATTTTTTAAGATGTTTAGTTCATGATATGTTAATAAGCCTTCAAATTTTCAGAGTTTTCAATTGGACAGAAAGAGTGGAAAAAAATGTTACATGCTTTCTGCTAGATCTCTTGCAATTGTTTGGGGTGATACTAAGCACTATTGGAACTGGATTCCTTTGTCTGATTCCAGGTTAGTTAGCGCTTAACTTTTCTATTGATTTTATTTTATTTCTTGTTCTCCGAATCAAACCGTTATATTTGAGCTAATAGCATAATATGTTATCTGCTATAAGTTAGCTTATCAGCTATAAGCTATCCACTATTTTTTATCAAACAAAGCCATATCCTTTTTTATGGACTTGAATGATTCTATTTACCATGTTAGTGATCAGTTAATGTTGATGGGTTTCAGGGCATTCAAAAAGAATAACAGGTTGAATATGCTCTTTCCTCATCTGCTAAGAATTAACTGACTTTTGCTATCTTCTAATCCAAGTTAGTGAAGAATAGTTGGTGTAGTGTTATAGGCCATACCATATATCAAAATCCATGGCTAGTAGCTACCACCTCGGGTTCAAACCCAAATTTAAAGCATGTTCAATCAAACAAATGCTTGCGAAAATTTCTCATGTCCTTGTGACTTAGAGAATTTGGAGTCTGTTCCTCCATATCACTTCAGCTAAGTATCAGCAACGTGTATGCGTGCTCTTCATTCAACTATCTTTTGCGTCCAACTTCAGCTTGGAGCTCCAAGATCCTTCTTCTGTAACTCTGCAGTTTTTGCTTCATCTGCAGACTTTCTTTTTTGATCCTAATTGCTGCGGAAACCGACCCTATATGATTTTGAATGCATCATTTTACCAAATTTTAAAATATCTGACCAATTTGTTTGTTACTTTACAACTAAAGATGTAGTTCTTATTTTAAGACTTCATCCTTAGTCCTTACACTATCTGGCCTATGTAGAAGCTTACTAGGCCACATGTGTGGACACTGGATCCACATTGAAGATGCACTACATGTAGCCATGTTTTCTTAGAAATTTTACTTTTTATCAAGAAATCCCATCGAATTACCCTGGCCATTCAATCTTTTGCGTAAGGTTGTCATTTGTCCAACTCCAACTCTTATTCTGTGACAATCATGCAGTTGTCTATATTGCTTATATCCACACTTATCACGAGAACCTGAGGCACATAGCAATGTCGATGTCCTTTTAGGCTTTTAGTATGGGAGAAGATGAATGTTGGATTTCCTAAAACGGCATATATAACTTATTCACTGAGAAGTCTGTCATTTAGTTTCAGCTTGAAATACTTAAGTTATGCTTCATAAAAGCCTCCAATTTTTAGTTAACGATCCTGCATTGTTAATTTATCGACCTTTTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGTGCACACACACACTCCATCTGTCATGCAATAAAACACTTTCAACGTTTCTCAGTTATTATGTTCTAATTTTTGCATCTTTTGTGTACTACTTTTGAACAGGTTCCCTGAAGTTGCTAAGCTTGTTGACGTATGTTGGCTTGAAATTCGTGGTGTGATAAACACCATTGTTCTGTCCCCAAATACTCAATATGCAGCTTATGTTGTGTTCAAAATGATTGATGCTTGGGGATTTCAGAACTGTCCTGTGGAGTTATCAGTTGGTGTCGAAGGTGGCCAGAGTAGTACTAAAATTGTCTGTTTGGATCCAAATGTGGAAGTGGAAGTGGAAGGTATGCAGCATAACAGAGCAGTAGGATTGCAACGTCCTAGTGTGAGAAGCGATGGGTGGTTGGAGATTGAGATGGGGGAATTCTTCAGTTCAGGCATAGAAAATGAAGAAGTCCTGATGAGTTTGTTGGAGATAAAGGCTCTTAATTGGAAGAGTGGTCTCTTTCTTGAAGGAATAGAAATTAGGCCTAAGTAA
Protein sequence

>MS.gene74873.t1

MTEIQDLPEGCIAAIVSRTTPLDAGRLSVVSKTFRSASDSDAVWNQFLPSDISSIISHSPSLANAPTKKALYLALSDRPIIIDHDQKSFQLDRKSGKKCYMLSARSLAIVWGDTKHYWNWIPLSDSRFPEVAKLVDVCWLEIRGVINTIVLSPNTQYAAYVVFKMIDAWGFQNCPVELSVGVEGGQSSTKIVCLDPNVEVEVEGMQHNRAVGLQRPSVRSDGWLEIEMGEFFSSGIENEEVLMSLLEIKALNWKSGLFLEGIEIRPK