AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49186

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49186.t1 MTR_1g100613 92.500 240 17 1 20 258 5 244 7.09e-164 454
MS.gene49186.t1 MTR_1g100597 82.437 279 44 3 1 278 1 275 1.02e-163 455
MS.gene49186.t1 MTR_1g079180 71.223 278 64 3 1 275 1 265 2.49e-139 393
MS.gene49186.t1 MTR_1g079370 71.739 276 66 3 1 275 1 265 9.67e-139 392
MS.gene49186.t1 MTR_1g079230 68.571 280 82 3 1 278 1 276 1.18e-134 382
MS.gene49186.t1 MTR_1g079260 68.571 280 81 3 1 278 1 275 4.94e-134 380
MS.gene49186.t1 MTR_1g079460 67.376 282 75 3 1 278 1 269 3.73e-130 370
MS.gene49186.t1 MTR_1g079850 67.014 288 81 5 1 278 1 284 1.38e-126 362
MS.gene49186.t1 MTR_1g079200 66.904 281 70 5 1 275 1 264 6.38e-125 357
MS.gene49186.t1 MTR_1g079380 69.565 276 72 3 1 275 1 265 2.89e-123 353
MS.gene49186.t1 MTR_3g006850 61.716 303 89 6 3 278 16 318 1.34e-115 335
MS.gene49186.t1 MTR_1g079250 63.448 290 85 7 1 278 1 281 3.85e-110 320
MS.gene49186.t1 MTR_3g452600 59.783 276 95 3 4 278 10 270 1.90e-105 307
MS.gene49186.t1 MTR_1g026510 57.534 292 104 8 1 278 1 286 5.99e-103 302
MS.gene49186.t1 MTR_1g079410 56.115 278 102 6 5 275 9 273 2.46e-96 284
MS.gene49186.t1 MTR_2g079100 58.242 273 92 4 3 272 8 261 9.72e-95 280
MS.gene49186.t1 MTR_3g450990 53.061 294 102 5 4 278 10 286 4.09e-91 271
MS.gene49186.t1 MTR_1g079350 52.297 283 119 8 1 275 1 275 2.52e-84 254
MS.gene49186.t1 MTR_1g079140 45.791 297 139 7 4 278 13 309 5.82e-80 244
MS.gene49186.t1 MTR_1g079340 50.836 299 110 8 1 275 40 325 7.74e-79 241
MS.gene49186.t1 MTR_1g079400 45.361 291 117 6 4 275 12 279 6.86e-77 235
MS.gene49186.t1 MTR_7g096300 54.545 264 89 8 1 255 1 242 3.82e-75 243
MS.gene49186.t1 MTR_1g078470 63.043 184 51 4 93 275 17 184 4.21e-70 214
MS.gene49186.t1 MTR_7g112550 43.110 283 148 7 2 275 5 283 1.00e-67 212
MS.gene49186.t1 MTR_1g083580 39.427 279 161 5 2 275 6 281 1.74e-64 203
MS.gene49186.t1 MTR_1g079240 57.143 189 60 3 87 275 12 179 1.22e-60 190
MS.gene49186.t1 MTR_7g112560 37.193 285 140 9 4 276 1 258 9.05e-55 178
MS.gene49186.t1 MTR_1g076870 67.164 134 38 3 128 255 2 135 6.91e-54 171
MS.gene49186.t1 MTR_4g023570 35.766 274 137 5 4 275 1 237 2.46e-49 163
MS.gene49186.t1 MTR_1g079210 64.463 121 42 1 129 248 7 127 8.13e-42 140
MS.gene49186.t1 MTR_1g079350 56.391 133 53 2 1 128 1 133 7.28e-41 141
MS.gene49186.t1 MTR_1g079130 44.503 191 88 6 101 275 1 189 6.99e-39 135
MS.gene49186.t1 MTR_7g096310 57.480 127 40 3 67 186 44 163 7.92e-37 129
MS.gene49186.t1 MTR_1g078460 70.115 87 26 0 1 87 64 150 6.22e-36 126
MS.gene49186.t1 MTR_2g042540 60.000 105 40 2 1 103 1 105 6.41e-34 120
MS.gene49186.t1 MTR_3g058640 54.867 113 38 1 128 240 7 106 9.99e-32 114
MS.gene49186.t1 MTR_0113s0040 26.389 288 167 5 2 275 20 276 1.02e-27 108
MS.gene49186.t1 MTR_7g103380 24.912 285 176 6 6 274 21 283 2.80e-27 107
MS.gene49186.t1 MTR_1g101390 28.571 280 169 8 2 274 26 281 7.49e-24 98.2
MS.gene49186.t1 MTR_0002s0020 27.957 279 165 7 6 272 24 278 1.68e-22 94.4
MS.gene49186.t1 MTR_4g102770 27.273 275 172 5 6 274 21 273 3.33e-21 90.9
MS.gene49186.t1 MTR_4g083970 25.862 232 138 5 48 276 91 291 7.90e-19 84.7
MS.gene49186.t1 MTR_5g010800 25.455 275 176 9 6 274 19 270 1.23e-17 81.3
MS.gene49186.t1 MTR_1g079220 54.430 79 23 1 165 243 1 66 1.78e-17 75.5
MS.gene49186.t1 MTR_1g079390 63.265 49 17 1 222 270 8 55 3.91e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49186.t1 AT2G02360 42.545 275 134 6 4 274 14 268 3.91e-69 215
MS.gene49186.t1 AT2G02240 42.647 272 139 4 4 274 61 316 1.12e-63 202
MS.gene49186.t1 AT1G80110 42.804 271 135 7 6 274 3 255 2.81e-63 199
MS.gene49186.t1 AT2G02250 41.455 275 139 6 4 274 47 303 8.36e-61 195
MS.gene49186.t1 AT2G02340 41.219 279 143 6 4 274 36 301 2.15e-59 191
MS.gene49186.t1 AT1G09155 41.197 284 146 8 7 275 3 280 7.91e-59 189
MS.gene49186.t1 AT2G02230 36.039 308 161 6 4 278 32 336 1.40e-58 190
MS.gene49186.t1 AT1G56250 40.071 282 154 7 7 276 4 282 5.12e-54 177
MS.gene49186.t1 AT1G56240 39.286 280 155 7 7 274 4 280 3.53e-52 172
MS.gene49186.t1 AT2G02350 35.612 278 157 7 2 274 22 282 8.28e-50 166
MS.gene49186.t1 AT2G02320 36.462 277 148 7 6 274 42 298 5.16e-48 162
MS.gene49186.t1 AT2G02310 33.452 281 157 5 4 275 45 304 7.28e-46 156
MS.gene49186.t1 AT2G02300 35.587 281 139 9 4 275 34 281 6.44e-42 145
MS.gene49186.t1 AT5G24560 41.146 192 100 4 4 192 3 184 1.71e-41 142
MS.gene49186.t1 AT3G53000 25.862 290 178 6 3 277 20 287 8.77e-27 106
MS.gene49186.t1 AT3G61060 26.259 278 173 8 6 275 27 280 2.71e-24 99.4
MS.gene49186.t1 AT3G61060 26.165 279 173 9 6 275 27 281 6.06e-23 95.9
MS.gene49186.t1 AT1G12710 24.912 285 171 5 3 274 27 281 5.10e-22 93.2
MS.gene49186.t1 AT5G52120 29.617 287 165 11 1 274 18 280 7.35e-22 92.8
MS.gene49186.t1 AT2G02350 31.285 179 104 5 101 274 1 165 3.31e-21 88.6
MS.gene49186.t1 AT1G63090 31.553 206 122 6 3 192 25 227 3.47e-21 90.9
MS.gene49186.t1 AT4G19850 29.474 190 96 5 94 275 17 176 1.23e-17 79.0
MS.gene49186.t1 AT4G19850 29.474 190 96 5 94 275 59 218 1.49e-17 79.7
MS.gene49186.t1 AT1G12710 30.818 159 108 2 3 160 27 184 1.92e-15 75.5
MS.gene49186.t1 AT4G19840 26.971 241 128 9 54 275 34 245 7.18e-14 70.1
MS.gene49186.t1 AT4G19850 41.975 81 41 2 94 168 59 139 1.90e-12 65.5

Find 81 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGCTGCATATTGAGTATT+TGG 0.137691 1.1:-72414870 None:intergenic
CTAGATCTCTTGCCATTACT+TGG 0.259697 1.1:+72413502 MS.gene49186:CDS
TAGCACTAAAATTGTTTGTT+TGG 0.300698 1.1:+72414974 MS.gene49186:CDS
ATGGGAGAGTTCTTCAATTC+AGG 0.321358 1.1:+72415122 MS.gene49186:CDS
CATTATCGACAATGGCAAAA+AGG 0.323000 1.1:+72413321 MS.gene49186:CDS
AAGCTGCATATTGAGTATTT+GGG 0.339648 1.1:-72414869 None:intergenic
CCTCCGATTCCGACGCCGTC+TGG 0.342753 1.1:+72413191 MS.gene49186:CDS
TTGCCATTGTCGATAATGAT+AGG 0.347824 1.1:-72413316 None:intergenic
GATCAGATAGGGCAAGATAA+AGG 0.356751 1.1:-72413291 None:intergenic
GGATTGCTATGCCTGATTCC+AGG 0.362529 1.1:+72413550 MS.gene49186:CDS
AGACGAAGTTTAGCAACTTC+GGG 0.364842 1.1:-72414798 None:intergenic
TGTAAATGCTCCTTCCAAAA+AGG 0.366929 1.1:+72413267 MS.gene49186:CDS
ATGACAACCGCTACTGGAAC+TGG 0.371010 1.1:+72413529 MS.gene49186:CDS
TATGAGTGTTATCGAGATAA+AGG 0.385187 1.1:+72415163 MS.gene49186:CDS
ATGTATACGAATTCCGCATT+TGG 0.390839 1.1:-72414999 None:intergenic
GAAAATGCAGAGCTTTCAAT+TGG 0.391080 1.1:+72413443 MS.gene49186:intron
TTCTCGAAGGAATAGAAGTT+AGG 0.399223 1.1:+72415210 MS.gene49186:CDS
TTCAAAATGATTGATTCCTA+TGG 0.400444 1.1:+72414900 MS.gene49186:CDS
TTAAGATCAAACCTGGAATC+AGG 0.407191 1.1:-72413561 None:intergenic
TAATGATAGGTTGATCAGAT+AGG 0.414184 1.1:-72413303 None:intergenic
AGGTCAGATAACAGAGTAGT+AGG 0.418025 1.1:+72415053 MS.gene49186:CDS
GGGGTGATGACAACCGCTAC+TGG 0.422453 1.1:+72413523 MS.gene49186:CDS
CGGAGTCGTACGAGACAGTA+TGG 0.434692 1.1:-72413122 None:intergenic
TTATCTCGATAACACTCATA+TGG 0.435546 1.1:-72415160 None:intergenic
TAGATCTCTTGCCATTACTT+GGG 0.440419 1.1:+72413503 MS.gene49186:CDS
GTTGCTTAATTGTCTACCTT+AGG 0.444416 1.1:-72415233 None:intergenic
ATCAGATAGGGCAAGATAAA+GGG 0.457911 1.1:-72413290 None:intergenic
ATTCCAGACGGCGTCGGAAT+CGG 0.462563 1.1:-72413194 None:intergenic
ACGATAGAGTCGACGTCGGA+AGG 0.464466 1.1:-72413226 None:intergenic
AAGACGAAGTTTAGCAACTT+CGG 0.467908 1.1:-72414799 None:intergenic
GAGAATCTACCGGCGTCAAC+CGG 0.472067 1.1:-72413142 None:intergenic
TAGGATTGCCGCGGCCTAGT+TGG 0.483978 1.1:+72415072 MS.gene49186:CDS
TCAAAATGATTGATTCCTAT+GGG 0.503514 1.1:+72414901 MS.gene49186:CDS
CGATAGAGTCGACGTCGGAA+GGG 0.510665 1.1:-72413225 None:intergenic
GTTGGAGAAGTGGTAGGTGG+TGG 0.510954 1.1:+72415090 MS.gene49186:CDS
GACAGGGTTTGAAGATTTAG+CGG 0.514285 1.1:+72413084 MS.gene49186:CDS
GGAGGCAGAAAGGAAAGTCT+TGG 0.523752 1.1:-72413173 None:intergenic
AGGAATAGAAGTTAGGCCTA+AGG 0.524720 1.1:+72415217 MS.gene49186:CDS
TAGGTGGTGGGAGATTGAGA+TGG 0.526412 1.1:+72415103 MS.gene49186:CDS
GATTTATCTGTCGGTGTCGA+AGG 0.528126 1.1:+72414942 MS.gene49186:CDS
AGGTGGTGGGAGATTGAGAT+GGG 0.529389 1.1:+72415104 MS.gene49186:CDS
AATGATAGGTTGATCAGATA+GGG 0.535784 1.1:-72413302 None:intergenic
AGATCTCTTGCCATTACTTG+GGG 0.535874 1.1:+72413504 MS.gene49186:CDS
TTGGAGAAGTGGTAGGTGGT+GGG 0.536804 1.1:+72415091 MS.gene49186:CDS
GGGTTTGAAGATTTAGCGGA+AGG 0.539826 1.1:+72413088 MS.gene49186:CDS
GTGTGGTGGCTCGAAATTCG+TGG 0.547967 1.1:+72414822 MS.gene49186:CDS
CTTCCTGTAGATTTATCTGT+CGG 0.550684 1.1:+72414933 MS.gene49186:CDS
AAGGGCAATTTCTTTCTCGA+AGG 0.553494 1.1:+72415197 MS.gene49186:CDS
CACCTACCACTTCTCCAACT+AGG 0.555718 1.1:-72415086 None:intergenic
GTTGTCATCACCCCAAGTAA+TGG 0.556839 1.1:-72413514 None:intergenic
ACACCGACAGATAAATCTAC+AGG 0.556842 1.1:-72414936 None:intergenic
GATAAAGGCTGGCGAGACAA+AGG 0.558520 1.1:+72415178 MS.gene49186:CDS
ATAAAGGCTGGCGAGACAAA+GGG 0.561504 1.1:+72415179 MS.gene49186:CDS
CTAAACTTCGTCTTGTGTGG+TGG 0.562220 1.1:+72414808 MS.gene49186:CDS
TTGCTAAACTTCGTCTTGTG+TGG 0.565369 1.1:+72414805 MS.gene49186:CDS
CATACTGTCTCGTACGACTC+CGG 0.570766 1.1:+72413123 MS.gene49186:CDS
AGTGTTATCGAGATAAAGGC+TGG 0.573301 1.1:+72415167 MS.gene49186:CDS
CCAGACGGCGTCGGAATCGG+AGG 0.576075 1.1:-72413191 None:intergenic
CGTCGGAATCGGAGGCAGAA+AGG 0.579217 1.1:-72413183 None:intergenic
CCGCGGCCTAGTTGGAGAAG+TGG 0.590220 1.1:+72415080 MS.gene49186:CDS
CAACCTATCATTATCGACAA+TGG 0.600808 1.1:+72413313 MS.gene49186:CDS
AGCTGCATATTGAGTATTTG+GGG 0.601395 1.1:-72414868 None:intergenic
AGGAAGGTTCTCAAACCCAT+AGG 0.604433 1.1:-72414916 None:intergenic
ATATTGAGTATTTGGGGACA+AGG 0.608473 1.1:-72414862 None:intergenic
TGTTTGTTTGGATCCAAATG+CGG 0.611023 1.1:+72414986 MS.gene49186:CDS
TGTGGTGGCTCGAAATTCGT+GGG 0.618223 1.1:+72414823 MS.gene49186:CDS
TTATCTGTCGGTGTCGAAGG+TGG 0.618376 1.1:+72414945 MS.gene49186:CDS
TAGCAATCCAGTTCCAGTAG+CGG 0.650859 1.1:-72413536 None:intergenic
AAAATGATTCCAGACGGCGT+CGG 0.652221 1.1:-72413200 None:intergenic
CTAGTTGGAGAAGTGGTAGG+TGG 0.655061 1.1:+72415087 MS.gene49186:CDS
TTCGTATACATCAGAGACAG+AGG 0.658529 1.1:+72415011 MS.gene49186:CDS
CGTACGACTCCGGTTGACGC+CGG 0.662520 1.1:+72413133 MS.gene49186:CDS
GGCCTAGTTGGAGAAGTGGT+AGG 0.668040 1.1:+72415084 MS.gene49186:CDS
GACGAAGTTTAGCAACTTCG+GGG 0.672634 1.1:-72414797 None:intergenic
GGAAACGACAGAGAATCTAC+CGG 0.672922 1.1:-72413152 None:intergenic
AGGGAGAAAATGATTCCAGA+CGG 0.678403 1.1:-72413206 None:intergenic
ACAGAGTAGTAGGATTGCCG+CGG 0.680550 1.1:+72415063 MS.gene49186:CDS
CGACAGATAAATCTACAGGA+AGG 0.687255 1.1:-72414932 None:intergenic
CCACTTCTCCAACTAGGCCG+CGG 0.703895 1.1:-72415080 None:intergenic
TGAGACGATAGAGTCGACGT+CGG 0.756214 1.1:-72413230 None:intergenic
AGTCGTACGAGACAGTATGG+TGG 0.758050 1.1:-72413119 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATATGATTGTTTATCA+TGG - chr1.1:72413815-72413834 None:intergenic 15.0%
!! AAAATGAAGGGAAAAAAAAT+TGG - chr1.1:72413998-72414017 None:intergenic 20.0%
!! AATAAAAGTGAGAAAATGAA+GGG - chr1.1:72414010-72414029 None:intergenic 20.0%
!! ATATGAAACATTCAATGAAA+TGG - chr1.1:72414101-72414120 None:intergenic 20.0%
!!! AGAAGAAAAATAAGTTGAAA+AGG - chr1.1:72414732-72414751 None:intergenic 20.0%
! AATGAAATGGAATGAACAAT+TGG - chr1.1:72414088-72414107 None:intergenic 25.0%
! AGGATTCATAACATCAAAAA+TGG - chr1.1:72414712-72414731 None:intergenic 25.0%
! CAATAAAAGTGAGAAAATGA+AGG - chr1.1:72414011-72414030 None:intergenic 25.0%
! CAATTCTTGTATTCTTATCT+CGG + chr1.1:72414752-72414771 MS.gene49186:intron 25.0%
! CTAGAGCTTAAAAAAATCAA+AGG - chr1.1:72413970-72413989 None:intergenic 25.0%
! GGAAATAATTTAGCTATCAT+TGG + chr1.1:72414521-72414540 MS.gene49186:intron 25.0%
! TAGCACTAAAATTGTTTGTT+TGG + chr1.1:72414974-72414993 MS.gene49186:CDS 25.0%
! TATGCAAGATGAAAAACAAT+GGG + chr1.1:72414174-72414193 MS.gene49186:intron 25.0%
!! ATTCATTGTTTTCTCATGTT+AGG + chr1.1:72413354-72413373 MS.gene49186:intron 25.0%
!! TCAAAATGATTGATTCCTAT+GGG + chr1.1:72414901-72414920 MS.gene49186:CDS 25.0%
!! TTATCATAATCGATTTCACT+TGG + chr1.1:72414500-72414519 MS.gene49186:intron 25.0%
!! TTCAAAATGATTGATTCCTA+TGG + chr1.1:72414900-72414919 MS.gene49186:CDS 25.0%
!!! AAACAATTTTAGTGCTACTA+TGG - chr1.1:72414971-72414990 None:intergenic 25.0%
!!! AAACATTTTTAGGTGTGTTT+GGG + chr1.1:72413693-72413712 MS.gene49186:intron 25.0%
!!! CTTCATTTTCTCACTTTTAT+TGG + chr1.1:72414009-72414028 MS.gene49186:intron 25.0%
!!! CTTTGATTTTTTTAAGCTCT+AGG + chr1.1:72413968-72413987 MS.gene49186:intron 25.0%
!!! GAGTTGTTTCAAAGTTTTTT+TGG + chr1.1:72414577-72414596 MS.gene49186:intron 25.0%
AAAAAATGAGGAGAAAGTGA+GGG - chr1.1:72414332-72414351 None:intergenic 30.0%
AAGCTGCATATTGAGTATTT+GGG - chr1.1:72414872-72414891 None:intergenic 30.0%
AGAAAAGTTAAGATCAAACC+TGG - chr1.1:72413571-72413590 None:intergenic 30.0%
ATGCTAGATGTGTAAATTGA+CGG + chr1.1:72413661-72413680 MS.gene49186:intron 30.0%
CTATGCAAGATGAAAAACAA+TGG + chr1.1:72414173-72414192 MS.gene49186:intron 30.0%
TAAAAAGAGTTATCTGACAG+TGG - chr1.1:72414440-72414459 None:intergenic 30.0%
TAAGCTGCATATTGAGTATT+TGG - chr1.1:72414873-72414892 None:intergenic 30.0%
TACACATCTAGCATTCATAA+AGG - chr1.1:72413655-72413674 None:intergenic 30.0%
TATGAGTGTTATCGAGATAA+AGG + chr1.1:72415163-72415182 MS.gene49186:CDS 30.0%
TTATCTCGATAACACTCATA+TGG - chr1.1:72415163-72415182 None:intergenic 30.0%
TTGGGATTGAATAGTCTAAA+AGG - chr1.1:72414371-72414390 None:intergenic 30.0%
TTTAGACTATTCAATCCCAA+AGG + chr1.1:72414371-72414390 MS.gene49186:intron 30.0%
! GATTTTGCAATCGTTTGATA+AGG + chr1.1:72415033-72415052 MS.gene49186:CDS 30.0%
! GCATTTTCAATATACCCAAA+GGG - chr1.1:72413431-72413450 None:intergenic 30.0%
! TCATATGGACTTCATTTTCA+AGG - chr1.1:72415148-72415167 None:intergenic 30.0%
! TGCATTTTCAATATACCCAA+AGG - chr1.1:72413432-72413451 None:intergenic 30.0%
! TTACAATTCTCCTCATTTTC+TGG + chr1.1:72413942-72413961 MS.gene49186:intron 30.0%
! TTGTTTTCTCATGTTAGGTA+AGG + chr1.1:72413359-72413378 MS.gene49186:intron 30.0%
!! AATGATAGGTTGATCAGATA+GGG - chr1.1:72413305-72413324 None:intergenic 30.0%
!! GTTAGTACTCTTTTGAAAGT+TGG + chr1.1:72414615-72414634 MS.gene49186:intron 30.0%
!! TAATGATAGGTTGATCAGAT+AGG - chr1.1:72413306-72413325 None:intergenic 30.0%
!!! AACATTTTTAGGTGTGTTTG+GGG + chr1.1:72413694-72413713 MS.gene49186:intron 30.0%
!!! CAAACATTTTTAGGTGTGTT+TGG + chr1.1:72413692-72413711 MS.gene49186:intron 30.0%
!!! GATTTTTTTAAGCTCTAGGT+TGG + chr1.1:72413972-72413991 MS.gene49186:intron 30.0%
!!! TTTCTCCTCATTTTTTCTTC+TGG + chr1.1:72414336-72414355 MS.gene49186:intron 30.0%
AAGACGAAGTTTAGCAACTT+CGG - chr1.1:72414802-72414821 None:intergenic 35.0%
AATGAACAATTGGCCAAGAA+AGG - chr1.1:72414078-72414097 None:intergenic 35.0%
ACAGAACAGACATTTGTGTT+TGG - chr1.1:72413915-72413934 None:intergenic 35.0%
AGACATTTGTGTTTGGATTG+AGG - chr1.1:72413908-72413927 None:intergenic 35.0%
AGATGAAAGTTTCCTTGATG+AGG - chr1.1:72414155-72414174 None:intergenic 35.0%
AGCTGCATATTGAGTATTTG+GGG - chr1.1:72414871-72414890 None:intergenic 35.0%
ATATTGAGTATTTGGGGACA+AGG - chr1.1:72414865-72414884 None:intergenic 35.0%
ATCAAAGGAACCAGAAAATG+AGG - chr1.1:72413955-72413974 None:intergenic 35.0%
ATCAGATAGGGCAAGATAAA+GGG - chr1.1:72413293-72413312 None:intergenic 35.0%
ATGAAAGTTTCCTTGATGAG+GGG - chr1.1:72414153-72414172 None:intergenic 35.0%
ATGTATACGAATTCCGCATT+TGG - chr1.1:72415002-72415021 None:intergenic 35.0%
CAACCTATCATTATCGACAA+TGG + chr1.1:72413313-72413332 MS.gene49186:CDS 35.0%
CATTATCGACAATGGCAAAA+AGG + chr1.1:72413321-72413340 MS.gene49186:CDS 35.0%
CATTGTTCTTTCTCCTTTCT+TGG + chr1.1:72414062-72414081 MS.gene49186:intron 35.0%
CTGCTAATAATAAGCCCTTT+GGG + chr1.1:72413414-72413433 MS.gene49186:intron 35.0%
CTTCCTGTAGATTTATCTGT+CGG + chr1.1:72414933-72414952 MS.gene49186:CDS 35.0%
GAAAAAATGAGGAGAAAGTG+AGG - chr1.1:72414333-72414352 None:intergenic 35.0%
GAAAATGCAGAGCTTTCAAT+TGG + chr1.1:72413443-72413462 MS.gene49186:intron 35.0%
GACATTTGTGTTTGGATTGA+GGG - chr1.1:72413907-72413926 None:intergenic 35.0%
GATGAAAGTTTCCTTGATGA+GGG - chr1.1:72414154-72414173 None:intergenic 35.0%
GCTATCCAGAAGAAAAAATG+AGG - chr1.1:72414344-72414363 None:intergenic 35.0%
GTTAAAACATGCAAGAGAGA+AGG - chr1.1:72414043-72414062 None:intergenic 35.0%
TAGATCTCTTGCCATTACTT+GGG + chr1.1:72413503-72413522 MS.gene49186:CDS 35.0%
TGCTAGATGTGTAAATTGAC+GGG + chr1.1:72413662-72413681 MS.gene49186:intron 35.0%
TGTAAATGCTCCTTCCAAAA+AGG + chr1.1:72413267-72413286 MS.gene49186:CDS 35.0%
TGTTACAGAGGTTCAGATTT+GGG - chr1.1:72414407-72414426 None:intergenic 35.0%
TGTTTGTTTGGATCCAAATG+CGG + chr1.1:72414986-72415005 MS.gene49186:CDS 35.0%
TTAAGATCAAACCTGGAATC+AGG - chr1.1:72413564-72413583 None:intergenic 35.0%
TTCTCGAAGGAATAGAAGTT+AGG + chr1.1:72415210-72415229 MS.gene49186:CDS 35.0%
TTGCCATTGTCGATAATGAT+AGG - chr1.1:72413319-72413338 None:intergenic 35.0%
TTGGGTGTATAGTTTCCTTT+GGG - chr1.1:72414389-72414408 None:intergenic 35.0%
TTTGGGTGTATAGTTTCCTT+TGG - chr1.1:72414390-72414409 None:intergenic 35.0%
! GGTCACACTCAAACATTTTT+AGG + chr1.1:72413683-72413702 MS.gene49186:intron 35.0%
! TATCTCGGCAATTTTTGTGT+TGG + chr1.1:72414767-72414786 MS.gene49186:intron 35.0%
AAGGGCAATTTCTTTCTCGA+AGG + chr1.1:72415197-72415216 MS.gene49186:CDS 40.0%
ACACCGACAGATAAATCTAC+AGG - chr1.1:72414939-72414958 None:intergenic 40.0%
AGACGAAGTTTAGCAACTTC+GGG - chr1.1:72414801-72414820 None:intergenic 40.0%
AGATCTCTTGCCATTACTTG+GGG + chr1.1:72413504-72413523 MS.gene49186:CDS 40.0%
AGGAATAGAAGTTAGGCCTA+AGG + chr1.1:72415217-72415236 MS.gene49186:CDS 40.0%
AGGGAGAAAATGATTCCAGA+CGG - chr1.1:72413209-72413228 None:intergenic 40.0%
AGGTCAGATAACAGAGTAGT+AGG + chr1.1:72415053-72415072 MS.gene49186:CDS 40.0%
AGTGTTATCGAGATAAAGGC+TGG + chr1.1:72415167-72415186 MS.gene49186:CDS 40.0%
ATCTGAGTTCACACTGTTGA+TGG + chr1.1:72414119-72414138 MS.gene49186:intron 40.0%
ATGGGAGAGTTCTTCAATTC+AGG + chr1.1:72415122-72415141 MS.gene49186:CDS 40.0%
ATTCCCGGTACAAGAGATAA+GGG - chr1.1:72414219-72414238 None:intergenic 40.0%
CGACAGATAAATCTACAGGA+AGG - chr1.1:72414935-72414954 None:intergenic 40.0%
CTAGATCTCTTGCCATTACT+TGG + chr1.1:72413502-72413521 MS.gene49186:CDS 40.0%
CTGTTACAGAGGTTCAGATT+TGG - chr1.1:72414408-72414427 None:intergenic 40.0%
GACAGGGTTTGAAGATTTAG+CGG + chr1.1:72413084-72413103 MS.gene49186:CDS 40.0%
GATCAGATAGGGCAAGATAA+AGG - chr1.1:72413294-72413313 None:intergenic 40.0%
GCTGCTAATAATAAGCCCTT+TGG + chr1.1:72413413-72413432 MS.gene49186:intron 40.0%
GTACAAGAGATAAGGGCTAA+CGG - chr1.1:72414212-72414231 None:intergenic 40.0%
TTAGCCCTTATCTCTTGTAC+CGG + chr1.1:72414212-72414231 MS.gene49186:intron 40.0%
TTCGTATACATCAGAGACAG+AGG + chr1.1:72415011-72415030 MS.gene49186:CDS 40.0%
TTGCTAAACTTCGTCTTGTG+TGG + chr1.1:72414805-72414824 MS.gene49186:CDS 40.0%
! AAGAAAAGCCAGTTGATTCC+CGG - chr1.1:72414234-72414253 None:intergenic 40.0%
! AGATAAAGGGCCTTTTTGGA+AGG - chr1.1:72413280-72413299 None:intergenic 40.0%
!!! TTTTTAGGTGTGTTTGGGGA+TGG + chr1.1:72413698-72413717 MS.gene49186:intron 40.0%
AAAATGATTCCAGACGGCGT+CGG - chr1.1:72413203-72413222 None:intergenic 45.0%
AAATGAGGAGAAAGTGAGGG+AGG - chr1.1:72414329-72414348 None:intergenic 45.0%
AGGAAGGTTCTCAAACCCAT+AGG - chr1.1:72414919-72414938 None:intergenic 45.0%
ATAAAGGCTGGCGAGACAAA+GGG + chr1.1:72415179-72415198 MS.gene49186:CDS 45.0%
CTAAACTTCGTCTTGTGTGG+TGG + chr1.1:72414808-72414827 MS.gene49186:CDS 45.0%
GAACCTCTGTAACAGTGATG+TGG + chr1.1:72414413-72414432 MS.gene49186:intron 45.0%
GACGAAGTTTAGCAACTTCG+GGG - chr1.1:72414800-72414819 None:intergenic 45.0%
GATTCCCGGTACAAGAGATA+AGG - chr1.1:72414220-72414239 None:intergenic 45.0%
GGAAACGACAGAGAATCTAC+CGG - chr1.1:72413155-72413174 None:intergenic 45.0%
GGGTTTGAAGATTTAGCGGA+AGG + chr1.1:72413088-72413107 MS.gene49186:CDS 45.0%
GTTGTCATCACCCCAAGTAA+TGG - chr1.1:72413517-72413536 None:intergenic 45.0%
GTTTCCTTGATGAGGGGAAT+AGG - chr1.1:72414147-72414166 None:intergenic 45.0%
TAGCAATCCAGTTCCAGTAG+CGG - chr1.1:72413539-72413558 None:intergenic 45.0%
TAGCCCTTATCTCTTGTACC+GGG + chr1.1:72414213-72414232 MS.gene49186:intron 45.0%
! CGGCAATTTTTGTGTTGGAC+AGG + chr1.1:72414772-72414791 MS.gene49186:intron 45.0%
! GGCAAGATAAAGGGCCTTTT+TGG - chr1.1:72413284-72413303 None:intergenic 45.0%
!! GATTTATCTGTCGGTGTCGA+AGG + chr1.1:72414942-72414961 MS.gene49186:CDS 45.0%
ACAGAGTAGTAGGATTGCCG+CGG + chr1.1:72415063-72415082 MS.gene49186:CDS 50.0%
AGTCGTACGAGACAGTATGG+TGG - chr1.1:72413122-72413141 None:intergenic 50.0%
ATGACAACCGCTACTGGAAC+TGG + chr1.1:72413529-72413548 MS.gene49186:CDS 50.0%
CACCTACCACTTCTCCAACT+AGG - chr1.1:72415089-72415108 None:intergenic 50.0%
CATACTGTCTCGTACGACTC+CGG + chr1.1:72413123-72413142 MS.gene49186:CDS 50.0%
CTAGTTGGAGAAGTGGTAGG+TGG + chr1.1:72415087-72415106 MS.gene49186:CDS 50.0%
CTCTTGTACCGGGAATCAAC+TGG + chr1.1:72414223-72414242 MS.gene49186:intron 50.0%
GAAAGTGAGGGAGGCTGAAA+TGG - chr1.1:72414320-72414339 None:intergenic 50.0%
GATAAAGGCTGGCGAGACAA+AGG + chr1.1:72415178-72415197 MS.gene49186:CDS 50.0%
GGACCACATCACTGTTACAG+AGG - chr1.1:72414419-72414438 None:intergenic 50.0%
GGAGGCAGAAAGGAAAGTCT+TGG - chr1.1:72413176-72413195 None:intergenic 50.0%
GGATCCTATTCCCCTCATCA+AGG + chr1.1:72414140-72414159 MS.gene49186:intron 50.0%
GGATTGCTATGCCTGATTCC+AGG + chr1.1:72413550-72413569 MS.gene49186:CDS 50.0%
TAGGTGTGTTTGGGGATGGA+GGG + chr1.1:72413702-72413721 MS.gene49186:intron 50.0%
TGAGACGATAGAGTCGACGT+CGG - chr1.1:72413233-72413252 None:intergenic 50.0%
TGTGGTGGCTCGAAATTCGT+GGG + chr1.1:72414823-72414842 MS.gene49186:CDS 50.0%
TGTTTGGGGATGGAGGGTTT+AGG + chr1.1:72413708-72413727 MS.gene49186:intron 50.0%
TTAGGTGTGTTTGGGGATGG+AGG + chr1.1:72413701-72413720 MS.gene49186:intron 50.0%
TTGGAGAAGTGGTAGGTGGT+GGG + chr1.1:72415091-72415110 MS.gene49186:CDS 50.0%
!! AGGTGGTGGGAGATTGAGAT+GGG + chr1.1:72415104-72415123 MS.gene49186:CDS 50.0%
!! TAGGTGGTGGGAGATTGAGA+TGG + chr1.1:72415103-72415122 MS.gene49186:CDS 50.0%
!! TTATCTGTCGGTGTCGAAGG+TGG + chr1.1:72414945-72414964 MS.gene49186:CDS 50.0%
ACGATAGAGTCGACGTCGGA+AGG - chr1.1:72413229-72413248 None:intergenic 55.0%
CGATAGAGTCGACGTCGGAA+GGG - chr1.1:72413228-72413247 None:intergenic 55.0%
CGGAGTCGTACGAGACAGTA+TGG - chr1.1:72413125-72413144 None:intergenic 55.0%
GAGAATCTACCGGCGTCAAC+CGG - chr1.1:72413145-72413164 None:intergenic 55.0%
GGCCTAGTTGGAGAAGTGGT+AGG + chr1.1:72415084-72415103 MS.gene49186:CDS 55.0%
GTGTGGTGGCTCGAAATTCG+TGG + chr1.1:72414822-72414841 MS.gene49186:CDS 55.0%
GTTGGAGAAGTGGTAGGTGG+TGG + chr1.1:72415090-72415109 MS.gene49186:CDS 55.0%
TGGGGATGGAGGGTTTAGGA+GGG + chr1.1:72413712-72413731 MS.gene49186:intron 55.0%
TTGGGGATGGAGGGTTTAGG+AGG + chr1.1:72413711-72413730 MS.gene49186:intron 55.0%
! ATTCCAGACGGCGTCGGAAT+CGG - chr1.1:72413197-72413216 None:intergenic 55.0%
CCACTTCTCCAACTAGGCCG+CGG - chr1.1:72415083-72415102 None:intergenic 60.0%
CGTCGGAATCGGAGGCAGAA+AGG - chr1.1:72413186-72413205 None:intergenic 60.0%
GGAGGGTTTAGGAGGGGGAA+GGG + chr1.1:72413719-72413738 MS.gene49186:intron 60.0%
GGGATGGAGGGTTTAGGAGG+GGG + chr1.1:72413714-72413733 MS.gene49186:intron 60.0%
GGGGATGGAGGGTTTAGGAG+GGG + chr1.1:72413713-72413732 MS.gene49186:intron 60.0%
GGGGTGATGACAACCGCTAC+TGG + chr1.1:72413523-72413542 MS.gene49186:CDS 60.0%
GGTTTAGGAGGGGGAAGGGA+GGG + chr1.1:72413723-72413742 MS.gene49186:intron 60.0%
GTTTAGGAGGGGGAAGGGAG+GGG + chr1.1:72413724-72413743 MS.gene49186:intron 60.0%
TAGGATTGCCGCGGCCTAGT+TGG + chr1.1:72415072-72415091 MS.gene49186:CDS 60.0%
TGGAGGGTTTAGGAGGGGGA+AGG + chr1.1:72413718-72413737 MS.gene49186:intron 60.0%
CCGCGGCCTAGTTGGAGAAG+TGG + chr1.1:72415080-72415099 MS.gene49186:CDS 65.0%
CGTACGACTCCGGTTGACGC+CGG + chr1.1:72413133-72413152 MS.gene49186:CDS 65.0%
GGGTTTAGGAGGGGGAAGGG+AGG + chr1.1:72413722-72413741 MS.gene49186:intron 65.0%
CCTCCGATTCCGACGCCGTC+TGG + chr1.1:72413191-72413210 MS.gene49186:CDS 70.0%
! CCAGACGGCGTCGGAATCGG+AGG - chr1.1:72413194-72413213 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 72413082 72415250 72413082 ID=MS.gene49186
chr1.1 mRNA 72413082 72415250 72413082 ID=MS.gene49186.t1;Parent=MS.gene49186
chr1.1 exon 72413082 72413342 72413082 ID=MS.gene49186.t1.exon1;Parent=MS.gene49186.t1
chr1.1 CDS 72413082 72413342 72413082 ID=cds.MS.gene49186.t1;Parent=MS.gene49186.t1
chr1.1 exon 72413453 72413571 72413453 ID=MS.gene49186.t1.exon2;Parent=MS.gene49186.t1
chr1.1 CDS 72413453 72413571 72413453 ID=cds.MS.gene49186.t1;Parent=MS.gene49186.t1
chr1.1 exon 72414794 72415250 72414794 ID=MS.gene49186.t1.exon3;Parent=MS.gene49186.t1
chr1.1 CDS 72414794 72415250 72414794 ID=cds.MS.gene49186.t1;Parent=MS.gene49186.t1
Gene Sequence

>MS.gene49186

ATGACAGGGTTTGAAGATTTAGCGGAAGGATGCATTGCCACCATACTGTCTCGTACGACTCCGGTTGACGCCGGTAGATTCTCTGTCGTTTCCAAGACTTTCCTTTCTGCCTCCGATTCCGACGCCGTCTGGAATCATTTTCTCCCTTCCGACGTCGACTCTATCGTCTCACAGTCATCTTCAGTTGTAAATGCTCCTTCCAAAAAGGCCCTTTATCTTGCCCTATCTGATCAACCTATCATTATCGACAATGGCAAAAAGGTATCATCAGAATTCATTGTTTTCTCATGTTAGGTAAGGCTAAATCAGTGATTATTGATCATCATAATTCGCTGCTAATAATAAGCCCTTTGGGTATATTGAAAATGCAGAGCTTTCAATTGGACAGAAAGAGTGCAAAAAAGTGTTACATGCTTGCTGCTAGATCTCTTGCCATTACTTGGGGTGATGACAACCGCTACTGGAACTGGATTGCTATGCCTGATTCCAGGTTTGATCTTAACTTTTCTATTTGTTTTATTTTAATTGCGTTCATTAACTTAATTAACTCTTAATTGACATTTATCTGCACCTTTATGAATGCTAGATGTGTAAATTGACGGGTCACACTCAAACATTTTTAGGTGTGTTTGGGGATGGAGGGTTTAGGAGGGGGAAGGGAGGGGAGAATTTACTTTTCATTTTTAAATTACGCACACTATGAAATATTTTTTAAAAAGTTAACATATTGCCATGATAAACAATCATATAATACTTCAATCACACTTAGTTTATTTTAAAAAAACATCTTACATAATCTGTAATTTAAAAAAGAAAAGTAAACCCTCAATCCAAACACAAATGTCTGTTCTGTTTCTCTCTTACAATTCTCCTCATTTTCTGGTTCCTTTGATTTTTTTAAGCTCTAGGTTGGCCAATTTTTTTTCCCTTCATTTTCTCACTTTTATTGGTTTCTTTACCTTCTCTCTTGCATGTTTTAACATTGTTCTTTCTCCTTTCTTGGCCAATTGTTCATTCCATTTCATTGAATGTTTCATATCTGAGTTCACACTGTTGATGGATCCTATTCCCCTCATCAAGGAAACTTTCATCTATGCAAGATGAAAAACAATGGGAGATTGAATATGCCGTTAGCCCTTATCTCTTGTACCGGGAATCAACTGGCTTTTCTTCTATGAAAGATGAAAAAACATGAGAAGTTGAATATGCATAAAGAAGTTAAATGAAAATATTTTCCATTTCAGCCTCCCTCACTTTCTCCTCATTTTTTCTTCTGGATAGCACTTCCTTTTAGACTATTCAATCCCAAAGGAAACTATACACCCAAATCTGAACCTCTGTAACAGTGATGTGGTCCACTGTCAGATAACTCTTTTTATAGATAACAAACGATATAACTAACTTATTAAATGAGAAGTTTATCATAATCGATTTCACTTGGAAATAATTTAGCTATCATTGGTACAATATATGATAATTGATGTTTTTATTAGTTGAGTTGTTTCAAAGTTTTTTTGGTAAAAATATTTTGCTGTTAGTACTCTTTTGAAAGTTGGAAACTTCGATTCAACTTTGAAATTTTAGTTGATGAGCTTGCATTGTTAGTATATCTACATGTATAAATATTCCATTTTTGATGTTATGAATCCTTTTCAACTTATTTTTCTTCTCAATTCTTGTATTCTTATCTCGGCAATTTTTGTGTTGGACAGGTTCCCCGAAGTTGCTAAACTTCGTCTTGTGTGGTGGCTCGAAATTCGTGGGATGATTAACAATCTTTCCTTGTCCCCAAATACTCAATATGCAGCTTATCTTGTGTTCAAAATGATTGATTCCTATGGGTTTGAGAACCTTCCTGTAGATTTATCTGTCGGTGTCGAAGGTGGCCATAGTAGCACTAAAATTGTTTGTTTGGATCCAAATGCGGAATTCGTATACATCAGAGACAGAGGATTTTGCAATCGTTTGATAAGGTCAGATAACAGAGTAGTAGGATTGCCGCGGCCTAGTTGGAGAAGTGGTAGGTGGTGGGAGATTGAGATGGGAGAGTTCTTCAATTCAGGCCTTGAAAATGAAGTCCATATGAGTGTTATCGAGATAAAGGCTGGCGAGACAAAGGGCAATTTCTTTCTCGAAGGAATAGAAGTTAGGCCTAAGGTAGACAATTAA
Protein sequence

>MS.gene49186.t1

MTGFEDLAEGCIATILSRTTPVDAGRFSVVSKTFLSASDSDAVWNHFLPSDVDSIVSQSSSVVNAPSKKALYLALSDQPIIIDNGKKSFQLDRKSAKKCYMLAARSLAITWGDDNRYWNWIAMPDSRFPEVAKLRLVWWLEIRGMINNLSLSPNTQYAAYLVFKMIDSYGFENLPVDLSVGVEGGHSSTKIVCLDPNAEFVYIRDRGFCNRLIRSDNRVVGLPRPSWRSGRWWEIEMGEFFNSGLENEVHMSVIEIKAGETKGNFFLEGIEVRPKVDN