AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005068

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005068.t1 MTR_1g079850 66.899 287 92 2 1 287 1 284 8.11e-133 378
MS.gene005068.t1 MTR_1g079180 66.551 287 71 6 1 284 1 265 8.57e-128 364
MS.gene005068.t1 MTR_1g100597 64.460 287 90 5 1 287 1 275 1.46e-122 351
MS.gene005068.t1 MTR_1g079200 62.587 286 83 4 1 284 1 264 4.44e-118 340
MS.gene005068.t1 MTR_1g079370 62.324 284 88 4 1 284 1 265 1.45e-117 338
MS.gene005068.t1 MTR_1g079380 62.676 284 87 3 1 284 1 265 1.78e-116 337
MS.gene005068.t1 MTR_1g079460 61.672 287 92 4 1 287 1 269 1.71e-115 333
MS.gene005068.t1 MTR_1g079250 62.887 291 94 7 1 287 1 281 3.82e-114 331
MS.gene005068.t1 MTR_1g079230 59.450 291 99 6 1 287 1 276 5.76e-112 325
MS.gene005068.t1 MTR_1g079260 59.028 288 104 6 1 287 1 275 3.51e-109 317
MS.gene005068.t1 MTR_1g026510 56.229 297 109 9 1 287 1 286 7.26e-105 307
MS.gene005068.t1 MTR_1g100613 65.587 247 77 5 20 266 5 243 8.02e-104 303
MS.gene005068.t1 MTR_3g006850 55.375 307 109 6 4 287 17 318 2.26e-101 299
MS.gene005068.t1 MTR_3g452600 56.140 285 100 5 4 287 10 270 8.94e-99 291
MS.gene005068.t1 MTR_2g079100 56.318 277 92 5 7 281 12 261 2.92e-93 277
MS.gene005068.t1 MTR_1g079350 53.425 292 111 8 1 284 1 275 7.22e-89 266
MS.gene005068.t1 MTR_3g450990 50.831 301 107 6 4 287 10 286 9.42e-88 263
MS.gene005068.t1 MTR_1g079410 48.443 289 124 6 2 285 6 274 1.48e-82 249
MS.gene005068.t1 MTR_1g079340 51.689 296 121 7 1 284 40 325 5.75e-81 247
MS.gene005068.t1 MTR_7g096300 52.788 269 95 6 1 264 1 242 1.11e-77 250
MS.gene005068.t1 MTR_1g079140 43.234 303 151 6 2 287 11 309 1.01e-71 223
MS.gene005068.t1 MTR_1g078470 60.733 191 49 5 97 284 17 184 3.64e-69 212
MS.gene005068.t1 MTR_7g112550 43.793 290 145 10 2 284 5 283 1.14e-65 207
MS.gene005068.t1 MTR_1g079400 41.901 284 140 5 7 284 15 279 7.04e-61 194
MS.gene005068.t1 MTR_1g083580 38.194 288 161 9 2 284 6 281 8.48e-57 184
MS.gene005068.t1 MTR_1g079240 54.315 197 58 6 91 284 12 179 4.83e-55 176
MS.gene005068.t1 MTR_2g042540 68.103 116 34 2 1 116 1 113 1.97e-46 152
MS.gene005068.t1 MTR_7g112560 35.484 279 154 8 7 284 4 257 1.11e-44 152
MS.gene005068.t1 MTR_1g076870 56.618 136 53 2 133 264 2 135 1.79e-44 147
MS.gene005068.t1 MTR_1g079350 50.588 170 72 4 1 164 1 164 8.96e-43 146
MS.gene005068.t1 MTR_1g079210 56.349 126 51 2 134 258 7 129 5.71e-38 131
MS.gene005068.t1 MTR_1g079130 42.487 193 94 5 105 284 1 189 3.29e-37 131
MS.gene005068.t1 MTR_4g023570 33.453 278 141 7 7 284 4 237 4.26e-37 132
MS.gene005068.t1 MTR_7g096310 47.561 164 65 5 38 187 3 159 2.63e-32 117
MS.gene005068.t1 MTR_3g058640 50.000 116 42 1 133 248 7 106 1.34e-29 108
MS.gene005068.t1 MTR_1g078460 61.702 94 32 2 1 94 64 153 9.12e-28 105
MS.gene005068.t1 MTR_1g079220 59.259 81 17 1 170 250 1 65 2.59e-24 94.0
MS.gene005068.t1 MTR_7g103380 25.524 286 189 9 7 285 22 290 1.15e-21 92.4
MS.gene005068.t1 MTR_0113s0040 31.176 170 109 5 2 170 20 182 6.44e-20 87.8
MS.gene005068.t1 MTR_0002s0020 29.825 171 105 5 3 166 21 183 1.57e-17 80.9
MS.gene005068.t1 MTR_1g101390 31.373 153 97 4 2 152 26 172 1.69e-17 80.9
MS.gene005068.t1 MTR_5g010800 30.612 147 96 3 6 151 19 160 2.46e-17 80.5
MS.gene005068.t1 MTR_4g102770 30.000 150 96 4 6 152 21 164 6.34e-15 73.6
MS.gene005068.t1 MTR_1g079390 66.000 50 15 2 230 279 8 55 6.50e-13 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005068.t1 AT2G02360 40.283 283 138 7 4 283 14 268 8.50e-62 197
MS.gene005068.t1 AT1G80110 41.429 280 137 8 5 283 2 255 9.14e-61 194
MS.gene005068.t1 AT2G02240 37.234 282 149 7 4 283 61 316 2.03e-55 182
MS.gene005068.t1 AT2G02340 35.254 295 153 9 1 283 33 301 1.80e-52 174
MS.gene005068.t1 AT1G09155 39.789 284 159 8 7 284 3 280 3.64e-52 172
MS.gene005068.t1 AT2G02250 35.816 282 154 8 4 283 47 303 4.55e-52 173
MS.gene005068.t1 AT2G02230 33.229 319 168 9 2 287 30 336 7.86e-52 173
MS.gene005068.t1 AT1G56250 39.236 288 155 8 7 284 4 281 1.19e-49 166
MS.gene005068.t1 AT1G56240 38.732 284 160 7 7 283 4 280 2.77e-49 165
MS.gene005068.t1 AT2G02350 34.386 285 160 6 2 283 22 282 6.65e-47 159
MS.gene005068.t1 AT2G02310 32.632 285 165 6 4 285 45 305 5.70e-44 152
MS.gene005068.t1 AT2G02300 35.374 294 133 12 4 285 34 282 1.14e-42 148
MS.gene005068.t1 AT2G02320 34.507 284 151 8 7 283 43 298 2.98e-42 147
MS.gene005068.t1 AT5G24560 41.722 151 80 3 4 154 3 145 1.73e-33 121
MS.gene005068.t1 AT3G53000 33.333 147 91 4 7 152 24 164 7.75e-22 93.2
MS.gene005068.t1 AT2G02350 31.319 182 105 4 105 283 1 165 4.21e-21 88.6
MS.gene005068.t1 AT1G12710 33.793 145 91 3 7 151 31 170 2.77e-17 80.5
MS.gene005068.t1 AT1G63090 32.727 165 102 5 7 170 29 185 3.16e-17 80.1
MS.gene005068.t1 AT3G61060 29.091 165 109 5 7 170 28 185 1.80e-16 78.2
MS.gene005068.t1 AT5G52120 28.846 156 105 3 1 152 18 171 6.17e-15 73.9
MS.gene005068.t1 AT3G61060 28.916 166 109 6 7 170 28 186 6.80e-15 73.6
MS.gene005068.t1 AT1G12710 32.331 133 85 3 7 139 31 158 4.21e-12 65.9

Find 78 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCATTCTTGATGATAAT+TGG 0.098885 1.4:+64953728 None:intergenic
ACCGAGCAATATTGATTTAA+TGG 0.199236 1.4:+64952800 None:intergenic
AAGTACTAAAACTGTCTTAT+TGG 0.208433 1.4:-64952850 MS.gene005068:CDS
TCTTGGCATTGCTTGGGATA+TGG 0.241695 1.4:-64953549 MS.gene005068:CDS
ATGGGAGAGTTCTTTAATTT+AGG 0.272165 1.4:-64952693 MS.gene005068:CDS
TAAGCCGCATACTGAGTATT+TGG 0.291931 1.4:+64952954 None:intergenic
GGCGGCATAGCTTCCACATT+TGG 0.323609 1.4:+64952825 None:intergenic
CACCATTCTTGATGATAATT+GGG 0.341199 1.4:+64953729 None:intergenic
GAGAACCTGCCGGCCTCAAC+TGG 0.346528 1.4:+64953914 None:intergenic
CTAGATCTCTTGGCATTGCT+TGG 0.349116 1.4:-64953556 MS.gene005068:CDS
TGCCAACACTCCAACCAAAA+AGG 0.355711 1.4:-64953777 MS.gene005068:CDS
GAAACAAGCTCAGCAACTTC+GGG 0.356958 1.4:+64953026 None:intergenic
TGCATAGAAAGAGTGGAAAA+AGG 0.371008 1.4:-64953595 MS.gene005068:CDS
GACGATGAAGTCGATGAATT+GGG 0.385801 1.4:+64953820 None:intergenic
GGATTGCTATGCCTGATTCC+AGG 0.390951 1.4:-64953505 MS.gene005068:intron
TGGAGTCGTATGAGAGAGTA+TGG 0.393657 1.4:+64953934 None:intergenic
ATGGCGGCAACGCACTCTTC+TGG 0.394654 1.4:+64953953 None:intergenic
CCGCTGATTCCGACGCCGTA+TGG 0.412845 1.4:-64953865 MS.gene005068:CDS
AGACGATGAAGTCGATGAAT+TGG 0.414822 1.4:+64953819 None:intergenic
ATGCTTGCTGCTAGATCTCT+TGG 0.415529 1.4:-64953566 MS.gene005068:CDS
ACAATGCACAACAGAGTAGA+AGG 0.419604 1.4:-64952762 MS.gene005068:CDS
ATATGGATGATGATCGCTAT+TGG 0.445967 1.4:-64953532 MS.gene005068:CDS
TTGGGTGGTTACCTGGAATC+AGG 0.447521 1.4:+64953494 None:intergenic
AGAAACAAGCTCAGCAACTT+CGG 0.449217 1.4:+64953025 None:intergenic
TAGATCTCTTGGCATTGCTT+GGG 0.454015 1.4:-64953555 MS.gene005068:CDS
CAATATTGCTCGGTCATGCT+TGG 0.461553 1.4:-64952791 MS.gene005068:CDS
GTAAAACCTGGTCTGCTGTT+GGG 0.464946 1.4:-64952633 MS.gene005068:CDS
GAGGACAGTTCTGAAATCCT+TGG 0.468868 1.4:+64952907 None:intergenic
GTGTGGATGCTTGACATTAG+TGG 0.471464 1.4:-64953002 MS.gene005068:CDS
TATCATCAAGAATGGTGAAA+AGG 0.485783 1.4:-64953723 MS.gene005068:intron
GTTGGAGTGTTGGCAATGGA+AGG 0.492413 1.4:+64953785 None:intergenic
CGACTCCAGTTGAGGCCGGC+AGG 0.492779 1.4:-64953919 MS.gene005068:CDS
AGGACAGTTCTGAAATCCTT+GGG 0.493582 1.4:+64952908 None:intergenic
AGCAATATTGATTTAATGGT+GGG 0.495478 1.4:+64952804 None:intergenic
CGCCCAATTATCATCAAGAA+TGG 0.495686 1.4:-64953731 MS.gene005068:CDS
AAATTTCCCAACAGCAGACC+AGG 0.503021 1.4:+64952627 None:intergenic
CACCCATCGCTTCTCAAACT+CGG 0.504886 1.4:+64952729 None:intergenic
AGCGGCAGAACGGAATGTCT+TGG 0.509093 1.4:+64953883 None:intergenic
GGTAAAACCTGGTCTGCTGT+TGG 0.521350 1.4:-64952634 MS.gene005068:CDS
TTTGAGAAGCGATGGGTGGT+TGG 0.523663 1.4:-64952724 MS.gene005068:CDS
CGTCCGAGTTTGAGAAGCGA+TGG 0.525014 1.4:-64952732 MS.gene005068:CDS
TGGGTGGTTGGAGATTGAGA+TGG 0.532103 1.4:-64952712 MS.gene005068:CDS
AGGAATAGAAGTTAGACCTA+AGG 0.534122 1.4:-64952595 MS.gene005068:CDS
GGGTGGTTGGAGATTGAGAT+GGG 0.540728 1.4:-64952711 MS.gene005068:CDS
GAGTTATCAGTTGGTGTCAG+AGG 0.545530 1.4:-64952882 MS.gene005068:CDS
ATGATGATCGCTATTGGAAG+TGG 0.550020 1.4:-64953526 MS.gene005068:CDS
AGTCAAGATGAGTGTTACCC+AGG 0.555540 1.4:-64952655 MS.gene005068:CDS
ACCATTAAATCAATATTGCT+CGG 0.556459 1.4:-64952801 MS.gene005068:CDS
ACGATGAAGTCGATGAATTG+GGG 0.559295 1.4:+64953821 None:intergenic
AGGATTTCAGAACTGTCCTC+AGG 0.560242 1.4:-64952904 MS.gene005068:CDS
TTGCTGAGCTTGTTTCTGTG+TGG 0.563125 1.4:-64953019 MS.gene005068:CDS
TTTGGTTGGAGTGTTGGCAA+TGG 0.565580 1.4:+64953781 None:intergenic
TTCAAGATGTTTGATGCCCA+AGG 0.578861 1.4:-64952924 MS.gene005068:CDS
GGAGTGTTGGCAATGGAAGG+AGG 0.579339 1.4:+64953788 None:intergenic
GAGCAATATTGATTTAATGG+TGG 0.581098 1.4:+64952803 None:intergenic
ATTCAATTGCATAGAAAGAG+TGG 0.587159 1.4:-64953602 MS.gene005068:CDS
CTCTCATACGACTCCAGTTG+AGG 0.597505 1.4:-64953927 MS.gene005068:CDS
GTCCGAGTTTGAGAAGCGAT+GGG 0.601232 1.4:-64952731 MS.gene005068:CDS
TGTCTTATTGGATCCAAATG+TGG 0.605426 1.4:-64952838 MS.gene005068:CDS
CATACGACTCCAGTTGAGGC+CGG 0.606429 1.4:-64953923 MS.gene005068:CDS
GAGTATTTGGAGACAAGACA+CGG 0.607854 1.4:+64952967 None:intergenic
CCATACGGCGTCGGAATCAG+CGG 0.608739 1.4:+64953865 None:intergenic
TGTCCTCAGGAGTTATCAGT+TGG 0.614418 1.4:-64952891 MS.gene005068:CDS
AGTGTTACCCAGGTAAAACC+TGG 0.617166 1.4:-64952645 MS.gene005068:CDS
CGTCGGAATCAGCGGCAGAA+CGG 0.619760 1.4:+64953873 None:intergenic
GGAAACCACGGAGAACCTGC+CGG 0.620432 1.4:+64953904 None:intergenic
GTCTCCAAATACTCAGTATG+CGG 0.620795 1.4:-64952958 MS.gene005068:CDS
CGAGTTTGAGAAGCGATGGG+TGG 0.621386 1.4:-64952728 MS.gene005068:CDS
ACGGAGAAAGTGATTCCATA+CGG 0.626762 1.4:+64953850 None:intergenic
AATATTGATTTAATGGTGGG+CGG 0.632455 1.4:+64952807 None:intergenic
TGAGGCCGGCAGGTTCTCCG+TGG 0.647519 1.4:-64953909 MS.gene005068:CDS
AAACAAGCTCAGCAACTTCG+GGG 0.651309 1.4:+64953027 None:intergenic
AAAGTGATTCCATACGGCGT+CGG 0.655224 1.4:+64953856 None:intergenic
CGATGAAGTCGATGAATTGG+GGG 0.663283 1.4:+64953822 None:intergenic
ACGGAATGTCTTGGAAACCA+CGG 0.690249 1.4:+64953892 None:intergenic
AGTATTTGGAGACAAGACAC+GGG 0.691675 1.4:+64952968 None:intergenic
ACACCAACTGATAACTCCTG+AGG 0.713843 1.4:+64952888 None:intergenic
AGTCGTATGAGAGAGTATGG+CGG 0.764815 1.4:+64953937 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGTACTAAAACTGTCTTAT+TGG - chr1.4:64953708-64953727 MS.gene005068:intron 25.0%
! AAGTCTAGTATTCGAAAAAT+TGG + chr1.4:64952879-64952898 None:intergenic 25.0%
! ACCATTAAATCAATATTGCT+CGG - chr1.4:64953757-64953776 MS.gene005068:CDS 25.0%
! AGTTGCAAAGATAAGAATAA+AGG + chr1.4:64953494-64953513 None:intergenic 25.0%
! ATACATAGAATTATGAGACA+TGG + chr1.4:64953277-64953296 None:intergenic 25.0%
! CAAGTCATCAGATATATATT+GGG + chr1.4:64953085-64953104 None:intergenic 25.0%
! GTAATTCATAACATCACAAA+TGG + chr1.4:64953451-64953470 None:intergenic 25.0%
! GTCAATTAAGTCAATTAATG+AGG + chr1.4:64953112-64953131 None:intergenic 25.0%
! TCAAGTCATCAGATATATAT+TGG + chr1.4:64953086-64953105 None:intergenic 25.0%
!!! AGACAGTTTTAGTACTTTTA+TGG + chr1.4:64953705-64953724 None:intergenic 25.0%
!!! AGCAATATTGATTTAATGGT+GGG + chr1.4:64953757-64953776 None:intergenic 25.0%
!!! TCTTGACTTCTTTATTTTTG+AGG + chr1.4:64953891-64953910 None:intergenic 25.0%
!!! TTGGGAAATTTTTTTCTTGA+AGG - chr1.4:64953943-64953962 MS.gene005068:CDS 25.0%
ATTCAATTGCATAGAAAGAG+TGG - chr1.4:64952956-64952975 MS.gene005068:CDS 30.0%
GATCAATCAATAGTAAGTGT+CGG + chr1.4:64953213-64953232 None:intergenic 30.0%
TATCATCAAGAATGGTGAAA+AGG - chr1.4:64952835-64952854 MS.gene005068:CDS 30.0%
TCTAGTATTCGAAAAATTGG+CGG + chr1.4:64952876-64952895 None:intergenic 30.0%
TGAAACAATTTAGTTACGCT+TGG - chr1.4:64953359-64953378 MS.gene005068:intron 30.0%
TGTTATTGAGGTTCAGATTA+GGG + chr1.4:64953243-64953262 None:intergenic 30.0%
! ATGGGAGAGTTCTTTAATTT+AGG - chr1.4:64953865-64953884 MS.gene005068:CDS 30.0%
! CACAAATGGAGTATTTATAC+AGG + chr1.4:64953437-64953456 None:intergenic 30.0%
! CACCATTCTTGATGATAATT+GGG + chr1.4:64952832-64952851 None:intergenic 30.0%
! TCACCATTCTTGATGATAAT+TGG + chr1.4:64952833-64952852 None:intergenic 30.0%
!! AATATTGATTTAATGGTGGG+CGG + chr1.4:64953754-64953773 None:intergenic 30.0%
!! ACCGAGCAATATTGATTTAA+TGG + chr1.4:64953761-64953780 None:intergenic 30.0%
!! GAGCAATATTGATTTAATGG+TGG + chr1.4:64953758-64953777 None:intergenic 30.0%
!! TATGATGATGAAAAGTGTCT+CGG + chr1.4:64953327-64953346 None:intergenic 30.0%
AATCCCATAAAAACGTTGTG+TGG + chr1.4:64953157-64953176 None:intergenic 35.0%
ACTGCTATGAAGCACATATA+TGG - chr1.4:64953130-64953149 MS.gene005068:intron 35.0%
AGGAATAGAAGTTAGACCTA+AGG - chr1.4:64953963-64953982 MS.gene005068:CDS 35.0%
AGGTTCAGATTAGGGTTTAT+AGG + chr1.4:64953235-64953254 None:intergenic 35.0%
ATATATATTGGGTGGTTACC+TGG + chr1.4:64953074-64953093 None:intergenic 35.0%
ATATGGATGATGATCGCTAT+TGG - chr1.4:64953026-64953045 MS.gene005068:CDS 35.0%
CAGGCTCATCAACTAAAATT+TGG + chr1.4:64953395-64953414 None:intergenic 35.0%
CTGTTATTGAGGTTCAGATT+AGG + chr1.4:64953244-64953263 None:intergenic 35.0%
GTCATCAGATATATATTGGG+TGG + chr1.4:64953082-64953101 None:intergenic 35.0%
TCGGTAACTATCACTTACAT+AGG + chr1.4:64953194-64953213 None:intergenic 35.0%
TGCATAGAAAGAGTGGAAAA+AGG - chr1.4:64952963-64952982 MS.gene005068:CDS 35.0%
TGTAGATACACTAACAATGC+AGG + chr1.4:64953414-64953433 None:intergenic 35.0%
! TATCTTTGCAACTTTTGCGT+TGG - chr1.4:64953501-64953520 MS.gene005068:intron 35.0%
! TGTCTTATTGGATCCAAATG+TGG - chr1.4:64953720-64953739 MS.gene005068:intron 35.0%
ACAATGCACAACAGAGTAGA+AGG - chr1.4:64953796-64953815 MS.gene005068:CDS 40.0%
ACGATGAAGTCGATGAATTG+GGG + chr1.4:64952740-64952759 None:intergenic 40.0%
ACGGAGAAAGTGATTCCATA+CGG + chr1.4:64952711-64952730 None:intergenic 40.0%
AGAAACAAGCTCAGCAACTT+CGG + chr1.4:64953536-64953555 None:intergenic 40.0%
AGACGATGAAGTCGATGAAT+TGG + chr1.4:64952742-64952761 None:intergenic 40.0%
AGTATTTGGAGACAAGACAC+GGG + chr1.4:64953593-64953612 None:intergenic 40.0%
ATGATGATCGCTATTGGAAG+TGG - chr1.4:64953032-64953051 MS.gene005068:CDS 40.0%
CGCCCAATTATCATCAAGAA+TGG - chr1.4:64952827-64952846 MS.gene005068:CDS 40.0%
CTGAACCTCAATAACAGTGA+TGG - chr1.4:64953247-64953266 MS.gene005068:intron 40.0%
GACGATGAAGTCGATGAATT+GGG + chr1.4:64952741-64952760 None:intergenic 40.0%
GACTACCATCACTGTTATTG+AGG + chr1.4:64953255-64953274 None:intergenic 40.0%
GAGTATTTGGAGACAAGACA+CGG + chr1.4:64953594-64953613 None:intergenic 40.0%
GTCTCCAAATACTCAGTATG+CGG - chr1.4:64953600-64953619 MS.gene005068:CDS 40.0%
TAAGCCGCATACTGAGTATT+TGG + chr1.4:64953607-64953626 None:intergenic 40.0%
TAGATCTCTTGGCATTGCTT+GGG - chr1.4:64953003-64953022 MS.gene005068:CDS 40.0%
TTCAAGATGTTTGATGCCCA+AGG - chr1.4:64953634-64953653 MS.gene005068:intron 40.0%
! GGACCACACAACGTTTTTAT+GGG - chr1.4:64953151-64953170 MS.gene005068:intron 40.0%
! TGGACCACACAACGTTTTTA+TGG - chr1.4:64953150-64953169 MS.gene005068:intron 40.0%
!! AGATACAGAGCCTTTTTGGT+TGG + chr1.4:64952794-64952813 None:intergenic 40.0%
!! AGGACAGTTCTGAAATCCTT+GGG + chr1.4:64953653-64953672 None:intergenic 40.0%
AAACAAGCTCAGCAACTTCG+GGG + chr1.4:64953534-64953553 None:intergenic 45.0%
AAAGTGATTCCATACGGCGT+CGG + chr1.4:64952705-64952724 None:intergenic 45.0%
AAATTTCCCAACAGCAGACC+AGG + chr1.4:64953934-64953953 None:intergenic 45.0%
ACACCAACTGATAACTCCTG+AGG + chr1.4:64953673-64953692 None:intergenic 45.0%
ACGGAATGTCTTGGAAACCA+CGG + chr1.4:64952669-64952688 None:intergenic 45.0%
AGGATTTCAGAACTGTCCTC+AGG - chr1.4:64953654-64953673 MS.gene005068:intron 45.0%
AGTCAAGATGAGTGTTACCC+AGG - chr1.4:64953903-64953922 MS.gene005068:CDS 45.0%
AGTGTTACCCAGGTAAAACC+TGG - chr1.4:64953913-64953932 MS.gene005068:CDS 45.0%
CAATATTGCTCGGTCATGCT+TGG - chr1.4:64953767-64953786 MS.gene005068:CDS 45.0%
CGATGAAGTCGATGAATTGG+GGG + chr1.4:64952739-64952758 None:intergenic 45.0%
CTAGATCTCTTGGCATTGCT+TGG - chr1.4:64953002-64953021 MS.gene005068:CDS 45.0%
GAAACAAGCTCAGCAACTTC+GGG + chr1.4:64953535-64953554 None:intergenic 45.0%
GTAAAACCTGGTCTGCTGTT+GGG - chr1.4:64953925-64953944 MS.gene005068:CDS 45.0%
GTGTGGATGCTTGACATTAG+TGG - chr1.4:64953556-64953575 MS.gene005068:CDS 45.0%
TCTTGGCATTGCTTGGGATA+TGG - chr1.4:64953009-64953028 MS.gene005068:CDS 45.0%
TGCCAACACTCCAACCAAAA+AGG - chr1.4:64952781-64952800 MS.gene005068:CDS 45.0%
TGGAGTCGTATGAGAGAGTA+TGG + chr1.4:64952627-64952646 None:intergenic 45.0%
TGTCCTCAGGAGTTATCAGT+TGG - chr1.4:64953667-64953686 MS.gene005068:intron 45.0%
! AGCGAGATACAGAGCCTTTT+TGG + chr1.4:64952798-64952817 None:intergenic 45.0%
! AGTCGTATGAGAGAGTATGG+CGG + chr1.4:64952624-64952643 None:intergenic 45.0%
! ATGCTTGCTGCTAGATCTCT+TGG - chr1.4:64952992-64953011 MS.gene005068:CDS 45.0%
!! GAGGACAGTTCTGAAATCCT+TGG + chr1.4:64953654-64953673 None:intergenic 45.0%
!! GAGTTATCAGTTGGTGTCAG+AGG - chr1.4:64953676-64953695 MS.gene005068:intron 45.0%
!! TTGCTGAGCTTGTTTCTGTG+TGG - chr1.4:64953539-64953558 MS.gene005068:CDS 45.0%
!! TTTGGTTGGAGTGTTGGCAA+TGG + chr1.4:64952780-64952799 None:intergenic 45.0%
!!! AGCCTTTTTGGTTGGAGTGT+TGG + chr1.4:64952786-64952805 None:intergenic 45.0%
CACCCATCGCTTCTCAAACT+CGG + chr1.4:64953832-64953851 None:intergenic 50.0%
CGATGAATTGGGGGTCAGAA+CGG + chr1.4:64952730-64952749 None:intergenic 50.0%
CTCTCATACGACTCCAGTTG+AGG - chr1.4:64952631-64952650 MS.gene005068:CDS 50.0%
GGATTGCTATGCCTGATTCC+AGG - chr1.4:64953053-64953072 MS.gene005068:intron 50.0%
GGGTGGTTGGAGATTGAGAT+GGG - chr1.4:64953847-64953866 MS.gene005068:CDS 50.0%
GGTAAAACCTGGTCTGCTGT+TGG - chr1.4:64953924-64953943 MS.gene005068:CDS 50.0%
GTCCGAGTTTGAGAAGCGAT+GGG - chr1.4:64953827-64953846 MS.gene005068:CDS 50.0%
TGGGTGGTTGGAGATTGAGA+TGG - chr1.4:64953846-64953865 MS.gene005068:CDS 50.0%
TTGGGTGGTTACCTGGAATC+AGG + chr1.4:64953067-64953086 None:intergenic 50.0%
TTTGAGAAGCGATGGGTGGT+TGG - chr1.4:64953834-64953853 MS.gene005068:CDS 50.0%
! ACAGCAGACCAGGTTTTACC+TGG + chr1.4:64953924-64953943 None:intergenic 50.0%
! CAGCAGACCAGGTTTTACCT+GGG + chr1.4:64953923-64953942 None:intergenic 50.0%
!! GTTGGAGTGTTGGCAATGGA+AGG + chr1.4:64952776-64952795 None:intergenic 50.0%
AGCGGCAGAACGGAATGTCT+TGG + chr1.4:64952678-64952697 None:intergenic 55.0%
CATACGACTCCAGTTGAGGC+CGG - chr1.4:64952635-64952654 MS.gene005068:CDS 55.0%
CGAGTTTGAGAAGCGATGGG+TGG - chr1.4:64953830-64953849 MS.gene005068:CDS 55.0%
CGTCCGAGTTTGAGAAGCGA+TGG - chr1.4:64953826-64953845 MS.gene005068:CDS 55.0%
GGCGGCATAGCTTCCACATT+TGG + chr1.4:64953736-64953755 None:intergenic 55.0%
!! GGAGTGTTGGCAATGGAAGG+AGG + chr1.4:64952773-64952792 None:intergenic 55.0%
ATGGCGGCAACGCACTCTTC+TGG + chr1.4:64952608-64952627 None:intergenic 60.0%
CGTCGGAATCAGCGGCAGAA+CGG + chr1.4:64952688-64952707 None:intergenic 60.0%
GGAAACCACGGAGAACCTGC+CGG + chr1.4:64952657-64952676 None:intergenic 60.0%
! CCATACGGCGTCGGAATCAG+CGG + chr1.4:64952696-64952715 None:intergenic 60.0%
GAGAACCTGCCGGCCTCAAC+TGG + chr1.4:64952647-64952666 None:intergenic 65.0%
!! CCGCTGATTCCGACGCCGTA+TGG - chr1.4:64952693-64952712 MS.gene005068:CDS 65.0%
CGACTCCAGTTGAGGCCGGC+AGG - chr1.4:64952639-64952658 MS.gene005068:CDS 70.0%
TGAGGCCGGCAGGTTCTCCG+TGG - chr1.4:64952649-64952668 MS.gene005068:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 64952584 64953996 64952584 ID=MS.gene005068
chr1.4 mRNA 64952584 64953996 64952584 ID=MS.gene005068.t1;Parent=MS.gene005068
chr1.4 exon 64953724 64953996 64953724 ID=MS.gene005068.t1.exon1;Parent=MS.gene005068.t1
chr1.4 CDS 64953724 64953996 64953724 ID=cds.MS.gene005068.t1;Parent=MS.gene005068.t1
chr1.4 exon 64953506 64953627 64953506 ID=MS.gene005068.t1.exon2;Parent=MS.gene005068.t1
chr1.4 CDS 64953506 64953627 64953506 ID=cds.MS.gene005068.t1;Parent=MS.gene005068.t1
chr1.4 exon 64952584 64953052 64952584 ID=MS.gene005068.t1.exon3;Parent=MS.gene005068.t1
chr1.4 CDS 64952584 64953052 64952584 ID=cds.MS.gene005068.t1;Parent=MS.gene005068.t1
Gene Sequence

>MS.gene005068

ATGACAAAGTTCGATAAATTGCCAGAAGAGTGCGTTGCCGCCATACTCTCTCATACGACTCCAGTTGAGGCCGGCAGGTTCTCCGTGGTTTCCAAGACATTCCGTTCTGCCGCTGATTCCGACGCCGTATGGAATCACTTTCTCCGTTCTGACCCCCAATTCATCGACTTCATCGTCTCACACTCTCCTCCTTCCATTGCCAACACTCCAACCAAAAAGGCTCTGTATCTCGCTCTCTCTGATCGCCCAATTATCATCAAGAATGGTGAAAAGGTATTATTCTTTGTAACCGCCAATTTTTCGAATACTAGACTTATCATTGACTGTATAGAATGCTGATAAATAAGTTTTGTATGTGTTGAAATTCAGAGCATTCAATTGCATAGAAAGAGTGGAAAAAGGTGTGTCATGCTTGCTGCTAGATCTCTTGGCATTGCTTGGGATATGGATGATGATCGCTATTGGAAGTGGATTGCTATGCCTGATTCCAGGTAACCACCCAATATATATCTGATGACTTGATTACCTCATTAATTGACTTAATTGACTGCTATGAAGCACATATATGGACCACACAACGTTTTTATGGGATTGAATGATTTTATTTCCTATGTAAGTGATAGTTACCGACACTTACTATTGATTGATCCTATAAACCCTAATCTGAACCTCAATAACAGTGATGGTAGTCCATGTCTCATAATTCTATGTATGCATAAACAATCAAAACAACTTATTAACCGAGACACTTTTCATCATCATAATCAGTTACACTTGAAACAATTTAGTTACGCTTGGTTTACAGCTTCCAAATTTTAGTTGATGAGCCTGCATTGTTAGTGTATCTACACCTGTATAAATACTCCATTTGTGATGTTATGAATTACTTTCAACTTTTCTCTCAGCTCCTTTATTCTTATCTTTGCAACTTTTGCGTTGGATAGATTCCCCGAAGTTGCTGAGCTTGTTTCTGTGTGGATGCTTGACATTAGTGGCGAGATAAAAACCCGTGTCTTGTCTCCAAATACTCAGTATGCGGCTTATCTTGTGTTCAAGATGTTTGATGCCCAAGGATTTCAGAACTGTCCTCAGGAGTTATCAGTTGGTGTCAGAGGTAGCCATAAAAGTACTAAAACTGTCTTATTGGATCCAAATGTGGAAGCTATGCCGCCCACCATTAAATCAATATTGCTCGGTCATGCTTGGACTAGAACAATGCACAACAGAGTAGAAGGACTGCAACGTCCGAGTTTGAGAAGCGATGGGTGGTTGGAGATTGAGATGGGAGAGTTCTTTAATTTAGGCCTCAAAAATAAAGAAGTCAAGATGAGTGTTACCCAGGTAAAACCTGGTCTGCTGTTGGGAAATTTTTTTCTTGAAGGAATAGAAGTTAGACCTAAGGAAGAAAATTAA
Protein sequence

>MS.gene005068.t1

MTKFDKLPEECVAAILSHTTPVEAGRFSVVSKTFRSAADSDAVWNHFLRSDPQFIDFIVSHSPPSIANTPTKKALYLALSDRPIIIKNGEKSIQLHRKSGKRCVMLAARSLGIAWDMDDDRYWKWIAMPDSRFPEVAELVSVWMLDISGEIKTRVLSPNTQYAAYLVFKMFDAQGFQNCPQELSVGVRGSHKSTKTVLLDPNVEAMPPTIKSILLGHAWTRTMHNRVEGLQRPSLRSDGWLEIEMGEFFNLGLKNKEVKMSVTQVKPGLLLGNFFLEGIEVRPKEEN