AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74858

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74858.t1 MTR_1g079140 86.452 310 35 4 1 304 2 310 0.0 534
MS.gene74858.t1 MTR_1g079130 92.746 193 14 0 112 304 1 193 2.76e-131 371
MS.gene74858.t1 MTR_1g079260 52.397 292 113 8 12 300 4 272 2.48e-92 275
MS.gene74858.t1 MTR_1g079370 49.662 296 110 7 11 300 3 265 3.44e-90 270
MS.gene74858.t1 MTR_1g079380 49.829 293 114 7 11 300 3 265 4.10e-85 258
MS.gene74858.t1 MTR_1g100597 50.342 292 119 7 12 300 4 272 4.22e-83 252
MS.gene74858.t1 MTR_1g079180 48.148 297 115 9 10 300 2 265 8.90e-82 248
MS.gene74858.t1 MTR_1g079460 47.635 296 119 6 10 300 2 266 2.66e-81 247
MS.gene74858.t1 MTR_1g079200 47.797 295 116 7 11 300 3 264 5.79e-80 243
MS.gene74858.t1 MTR_1g079850 46.959 296 136 6 10 300 2 281 2.90e-77 237
MS.gene74858.t1 MTR_1g079410 45.875 303 127 8 5 300 1 273 5.55e-75 231
MS.gene74858.t1 MTR_1g079230 46.939 294 127 8 12 300 4 273 3.11e-74 229
MS.gene74858.t1 MTR_1g026510 43.854 301 136 7 12 300 4 283 3.57e-69 217
MS.gene74858.t1 MTR_1g079250 45.946 296 132 7 12 300 4 278 1.03e-68 216
MS.gene74858.t1 MTR_1g100613 46.743 261 113 5 28 283 5 244 2.41e-68 213
MS.gene74858.t1 MTR_7g112550 42.759 290 146 9 15 300 10 283 4.19e-66 209
MS.gene74858.t1 MTR_1g083580 41.463 287 151 6 15 300 11 281 1.44e-65 207
MS.gene74858.t1 MTR_3g452600 42.268 291 133 6 12 300 10 267 1.83e-65 207
MS.gene74858.t1 MTR_7g112560 41.115 287 135 5 15 300 4 257 5.33e-64 202
MS.gene74858.t1 MTR_1g079400 40.134 299 150 6 6 300 6 279 3.92e-62 198
MS.gene74858.t1 MTR_3g006850 43.182 308 147 9 12 300 17 315 1.62e-60 196
MS.gene74858.t1 MTR_4g023570 39.721 287 119 5 15 300 4 237 4.57e-60 192
MS.gene74858.t1 MTR_2g079100 39.655 290 130 6 14 297 11 261 6.52e-55 179
MS.gene74858.t1 MTR_1g079350 39.274 303 139 9 12 300 4 275 5.76e-54 177
MS.gene74858.t1 MTR_3g450990 37.540 309 138 9 12 300 10 283 7.64e-53 175
MS.gene74858.t1 MTR_7g096300 39.502 281 127 9 12 286 4 247 1.47e-43 159
MS.gene74858.t1 MTR_1g079340 37.417 302 157 9 12 300 43 325 2.69e-42 149
MS.gene74858.t1 MTR_1g078470 43.719 199 79 5 104 300 17 184 2.41e-41 142
MS.gene74858.t1 MTR_1g079240 41.667 204 82 6 98 300 12 179 4.84e-35 125
MS.gene74858.t1 MTR_1g079350 37.826 230 110 10 12 223 4 218 1.46e-30 115
MS.gene74858.t1 MTR_7g103380 27.517 298 169 7 14 299 21 283 4.08e-27 107
MS.gene74858.t1 MTR_1g076870 39.437 142 78 3 139 280 2 135 1.34e-23 94.4
MS.gene74858.t1 MTR_4g083970 26.721 247 132 7 56 300 91 290 1.45e-23 98.2
MS.gene74858.t1 MTR_5g010800 31.280 211 129 6 9 207 14 220 3.44e-22 94.4
MS.gene74858.t1 MTR_1g101390 36.364 143 87 2 14 155 30 169 2.29e-21 92.0
MS.gene74858.t1 MTR_0002s0020 29.048 210 132 4 14 208 24 231 3.10e-21 91.7
MS.gene74858.t1 MTR_0113s0040 25.837 209 137 4 14 209 24 227 2.72e-20 89.0
MS.gene74858.t1 MTR_7g096310 48.544 103 43 3 79 175 43 141 4.19e-20 85.9
MS.gene74858.t1 MTR_4g102770 36.806 144 86 3 14 155 21 161 9.00e-20 87.8
MS.gene74858.t1 MTR_2g042540 42.453 106 54 3 12 114 4 105 1.60e-17 77.8
MS.gene74858.t1 MTR_1g078460 47.778 90 38 3 12 98 67 150 4.03e-15 72.0
MS.gene74858.t1 MTR_1g079210 36.000 150 73 7 141 286 8 138 3.74e-12 63.2
MS.gene74858.t1 MTR_3g058640 34.127 126 57 4 139 264 7 106 1.71e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74858.t1 AT2G02250 43.643 291 127 7 12 299 47 303 6.49e-76 235
MS.gene74858.t1 AT2G02360 42.475 299 131 6 5 299 7 268 6.02e-75 231
MS.gene74858.t1 AT2G02230 40.645 310 155 7 12 300 32 333 6.19e-75 233
MS.gene74858.t1 AT2G02240 44.483 290 125 7 12 299 61 316 6.61e-74 230
MS.gene74858.t1 AT2G02340 42.761 297 134 7 10 299 34 301 4.79e-71 222
MS.gene74858.t1 AT1G80110 41.980 293 131 7 12 301 1 257 3.00e-65 206
MS.gene74858.t1 AT1G09155 40.550 291 157 6 15 301 3 281 1.64e-63 202
MS.gene74858.t1 AT1G56240 41.667 288 158 6 15 301 4 282 9.62e-62 197
MS.gene74858.t1 AT1G56250 41.812 287 157 5 15 300 4 281 6.22e-60 193
MS.gene74858.t1 AT2G02350 38.356 292 139 7 14 299 26 282 2.72e-53 176
MS.gene74858.t1 AT2G02320 38.333 300 146 10 8 302 36 301 4.05e-53 176
MS.gene74858.t1 AT2G02310 35.333 300 143 6 12 300 45 304 2.30e-52 174
MS.gene74858.t1 AT2G02300 35.197 304 140 9 5 300 27 281 3.30e-48 163
MS.gene74858.t1 AT5G24560 51.351 148 62 2 10 155 1 140 7.34e-47 156
MS.gene74858.t1 AT5G24560 60.784 51 19 1 250 300 143 192 2.33e-13 68.2
MS.gene74858.t1 AT3G53000 25.658 304 176 6 9 299 18 284 1.16e-27 109
MS.gene74858.t1 AT2G02350 34.375 192 95 5 112 299 1 165 8.32e-22 90.9
MS.gene74858.t1 AT3G61060 26.484 219 143 4 14 219 27 240 2.63e-21 92.0
MS.gene74858.t1 AT3G61060 26.364 220 143 5 14 219 27 241 9.17e-20 87.8
MS.gene74858.t1 AT1G12710 27.064 218 139 5 14 218 30 240 4.63e-17 80.1
MS.gene74858.t1 AT1G63090 27.064 218 139 5 14 218 28 238 5.55e-17 80.1
MS.gene74858.t1 AT5G52120 27.149 221 136 7 12 213 21 235 4.43e-16 77.4

Find 85 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATTTAAGTCGAGTGCTTC+TGG 0.240736 1.1:-58848671 MS.gene74858:CDS
TCATTCATTGAACATGATTT+AGG 0.268000 1.1:+58848287 None:intergenic
TTTGATTATTACCTTGAATC+TGG 0.280432 1.1:+58849432 None:intergenic
TTGTTCAAGGAATTGAAATA+AGG 0.306864 1.1:-58848316 MS.gene74858:CDS
CGAGGAGTTTGTTTATTGTA+TGG 0.313317 1.1:-58849491 MS.gene74858:CDS
CGCTGAATCTGATTCAGTTT+GGG 0.315197 1.1:-58849895 MS.gene74858:CDS
GTTCCTCGCTTCCGTACTAC+TGG 0.331275 1.1:-58848530 MS.gene74858:CDS
TGATGTTGCGAGACTCTCTT+TGG 0.340577 1.1:-58849940 MS.gene74858:CDS
GTAAGATAGAGATCCTTCTT+AGG 0.342690 1.1:+58849792 None:intergenic
CCGCTGAATCTGATTCAGTT+TGG 0.347831 1.1:-58849896 MS.gene74858:CDS
GTGTGTGTTGGTTCGAAATC+CGG 0.363384 1.1:-58848748 MS.gene74858:CDS
TGGGTGGTTGGAGATGGAAT+TGG 0.367950 1.1:-58848426 MS.gene74858:CDS
CACCCATCTGCTCTCTGCTT+TGG 0.374699 1.1:+58848443 None:intergenic
ATTGAACATGATTTAGGCTT+AGG 0.376062 1.1:+58848293 None:intergenic
ATATCATCTACACTTTCTAT+TGG 0.376774 1.1:+58848476 None:intergenic
TATGCTCCGTACAAAGTCTT+TGG 0.387723 1.1:+58848698 None:intergenic
GTGGAATTAGAGCTTTCAAT+TGG 0.395595 1.1:-58849550 MS.gene74858:intron
GATGATAAGGAGGTGGAGAT+AGG 0.395868 1.1:-58848380 MS.gene74858:CDS
GGGTGGTTGGAGATGGAATT+GGG 0.406130 1.1:-58848425 MS.gene74858:CDS
TGTGTGTTGGTTCGAAATCC+GGG 0.415746 1.1:-58848747 MS.gene74858:CDS
AGCAGATGGGTGGTTGGAGA+TGG 0.421763 1.1:-58848432 MS.gene74858:CDS
GAAGCCTCCATTGCCATTGC+TGG 0.422783 1.1:-58848620 MS.gene74858:CDS
GGGGAGATACTCCGAGGTAT+TGG 0.424153 1.1:-58849470 MS.gene74858:CDS
GAAGAAATGCTACATGTTAT+CGG 0.434918 1.1:-58849514 MS.gene74858:CDS
TGTGGAGCGAAATGTATTCT+TGG 0.443243 1.1:-58848588 MS.gene74858:CDS
AAGGGTGGTCTTGTTGTTCA+AGG 0.455014 1.1:-58848329 MS.gene74858:CDS
GAGGAGTTTGTTTATTGTAT+GGG 0.456794 1.1:-58849490 MS.gene74858:CDS
TATCCAAAGCAGAGAGCAGA+TGG 0.467845 1.1:-58848446 MS.gene74858:CDS
ATGAGGTCAAAGGTGATGGT+TGG 0.470254 1.1:-58848352 MS.gene74858:CDS
CAATGAAGGAGGAGATGATA+AGG 0.471085 1.1:-58848393 MS.gene74858:CDS
GGATTACTGTTCCAGATTCA+AGG 0.479818 1.1:-58849443 MS.gene74858:intron
GGTGGAGATAGGTGTTTATG+AGG 0.486130 1.1:-58848369 MS.gene74858:CDS
TTGGGAGAGTTCTTCAATGA+AGG 0.486931 1.1:-58848407 MS.gene74858:CDS
GTTATCTGTCCTTCCTAAGA+AGG 0.495092 1.1:-58849805 MS.gene74858:CDS
GTTGGTTCGAAATCCGGGGC+TGG 0.497772 1.1:-58848742 MS.gene74858:CDS
CTTTCTATTGGTTCTACTAG+TGG 0.505826 1.1:+58848488 None:intergenic
TTTCAATTGGATAGAGCTGA+TGG 0.510875 1.1:-58849537 MS.gene74858:CDS
AGGAGGAGATGATAAGGAGG+TGG 0.515981 1.1:-58848387 MS.gene74858:CDS
GCAGAGAGCAGATGGGTGGT+TGG 0.518028 1.1:-58848438 MS.gene74858:CDS
ATGGCTGATACTCAAATCCA+TGG 0.522370 1.1:+58848645 None:intergenic
TCTCATAGACGATGGCGAAA+AGG 0.522395 1.1:-58849757 MS.gene74858:intron
TACGGAAGCGAGGAACAATC+TGG 0.523651 1.1:+58848537 None:intergenic
TCGCCATCGTCTATGAGAAG+TGG 0.524813 1.1:+58849762 None:intergenic
GGTCAAAGGTGATGGTTGGA+AGG 0.524881 1.1:-58848348 MS.gene74858:CDS
CTATCTCCAAAGACTTTGTA+CGG 0.532540 1.1:-58848704 MS.gene74858:CDS
AAAGGTGATGGTTGGAAGGG+TGG 0.535241 1.1:-58848344 MS.gene74858:CDS
CTGAACTTGTAAGTGTGTGT+TGG 0.535998 1.1:-58848760 MS.gene74858:CDS
TTCCTCGCTTCCGTACTACT+GGG 0.539964 1.1:-58848529 MS.gene74858:CDS
GTGGTTGTTATTGAAGAAGA+AGG 0.544758 1.1:-58850026 MS.gene74858:CDS
GTCAAAGGTGATGGTTGGAA+GGG 0.548325 1.1:-58848347 MS.gene74858:CDS
AGGAGTTTGTTTATTGTATG+GGG 0.552502 1.1:-58849489 MS.gene74858:CDS
ACCTCCAGCAATGGCAATGG+AGG 0.552633 1.1:+58848616 None:intergenic
ATACTCCGAGGTATTGGAGA+TGG 0.558448 1.1:-58849464 MS.gene74858:CDS
CACAGTATCACCTCCAGCAA+TGG 0.558458 1.1:+58848607 None:intergenic
TGAAGGAGGAGATGATAAGG+AGG 0.559543 1.1:-58848390 MS.gene74858:CDS
TCGAGTGCTTCTGGAACCCA+TGG 0.562038 1.1:-58848662 MS.gene74858:CDS
GTATTCTTGGATGCGGAAAG+AGG 0.564554 1.1:-58848575 MS.gene74858:CDS
ATAGGTGTTTATGAGGTCAA+AGG 0.573961 1.1:-58848362 MS.gene74858:CDS
ATCACCTCCAGCAATGGCAA+TGG 0.578717 1.1:+58848613 None:intergenic
GATAGAGATCCTTCTTAGGA+AGG 0.586243 1.1:+58849796 None:intergenic
GAGTTTCATGATTTACCTGA+AGG 0.586911 1.1:-58849999 MS.gene74858:CDS
GCGAAATGTATTCTTGGATG+CGG 0.589696 1.1:-58848582 MS.gene74858:CDS
GGAGAGTTCTTCAATGAAGG+AGG 0.592604 1.1:-58848404 MS.gene74858:CDS
GAGTGAATATCCCAGTAGTA+CGG 0.602751 1.1:+58848519 None:intergenic
TGATAGTGTTGATCCAGCCC+CGG 0.609203 1.1:+58848729 None:intergenic
GTTTATGAGGTCAAAGGTGA+TGG 0.610839 1.1:-58848356 MS.gene74858:CDS
AACATCATGTGGTGTTGTGA+AGG 0.615808 1.1:+58849956 None:intergenic
GCCTCCATTGCCATTGCTGG+AGG 0.627688 1.1:-58848617 MS.gene74858:CDS
GAGAGTCTCGCAACATCATG+TGG 0.628568 1.1:+58849945 None:intergenic
AAGCACTCGACTTAAATACA+AGG 0.637381 1.1:+58848675 None:intergenic
TTCAATTGGATAGAGCTGAT+GGG 0.638399 1.1:-58849536 MS.gene74858:CDS
ATCCAAAGCAGAGAGCAGAT+GGG 0.644218 1.1:-58848445 MS.gene74858:CDS
TGGCTGATACTCAAATCCAT+GGG 0.644935 1.1:+58848646 None:intergenic
ATGGCAATGGAGGCTTCACA+TGG 0.646731 1.1:+58848626 None:intergenic
CCAAACTGAATCAGATTCAG+CGG 0.649311 1.1:+58849896 None:intergenic
ATCCCAGTAGTACGGAAGCG+AGG 0.653042 1.1:+58848527 None:intergenic
GTAATCCATCTCCAATACCT+CGG 0.657967 1.1:+58849459 None:intergenic
AACTGAATCAGATTCAGCGG+CGG 0.667643 1.1:+58849899 None:intergenic
CAAAGCAGAGAGCAGATGGG+TGG 0.672349 1.1:-58848442 MS.gene74858:CDS
AAACCACTTCTCATAGACGA+TGG 0.695513 1.1:-58849765 MS.gene74858:CDS
GTGTGTTGGTTCGAAATCCG+GGG 0.699450 1.1:-58848746 MS.gene74858:CDS
AATGCTACATGTTATCGGCG+AGG 0.704221 1.1:-58849509 MS.gene74858:CDS
TTGTATGGGGAGATACTCCG+AGG 0.719053 1.1:-58849476 MS.gene74858:CDS
CATTGCTGGAGGTGATACTG+TGG 0.727694 1.1:-58848606 MS.gene74858:CDS
TGGATGCGGAAAGAGGACGA+AGG 0.783182 1.1:-58848568 MS.gene74858:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACAATAAACGTTTAATTA+AGG - chr1.1:58849267-58849286 MS.gene74858:intron 15.0%
!! ATAGTTTCAATAACTAAATT+TGG - chr1.1:58849437-58849456 MS.gene74858:intron 15.0%
!! ATCAATTCAAAAAAAAAATC+AGG - chr1.1:58849516-58849535 MS.gene74858:CDS 15.0%
!! TTGTTACCTATATATTTCAA+TGG - chr1.1:58849476-58849495 MS.gene74858:CDS 20.0%
!!! AATTTACTATCTTTTTGTCT+CGG - chr1.1:58848579-58848598 MS.gene74858:CDS 20.0%
!!! ATTTTTGAAGTTGTAGATAT+TGG - chr1.1:58848962-58848981 MS.gene74858:intron 20.0%
! AAACATATCTTCGCTATTAA+TGG + chr1.1:58848688-58848707 None:intergenic 25.0%
! AATAAACTGAAAACAGCTTA+TGG + chr1.1:58849143-58849162 None:intergenic 25.0%
! ATAAACTGAAAACAGCTTAT+GGG + chr1.1:58849142-58849161 None:intergenic 25.0%
! ATATCATCTACACTTTCTAT+TGG + chr1.1:58849839-58849858 None:intergenic 25.0%
! ATTTATGAAATACGCATGTT+TGG - chr1.1:58849023-58849042 MS.gene74858:intron 25.0%
! CAATCACCATTGAAATATAT+AGG + chr1.1:58849485-58849504 None:intergenic 25.0%
! TAATAAGCATGGATCAATTT+AGG + chr1.1:58849310-58849329 None:intergenic 25.0%
! TCATTCATTGAACATGATTT+AGG + chr1.1:58850028-58850047 None:intergenic 25.0%
! TCTTAATGTACTAATAAGCA+TGG + chr1.1:58849321-58849340 None:intergenic 25.0%
! TTAATAGCGAAGATATGTTT+AGG - chr1.1:58848688-58848707 MS.gene74858:CDS 25.0%
! TTGTTCAAGGAATTGAAATA+AGG - chr1.1:58849996-58850015 MS.gene74858:CDS 25.0%
! TTTGATTATTACCTTGAATC+TGG + chr1.1:58848883-58848902 None:intergenic 25.0%
!! AAAAAAAATCAGGTTTTCTG+AGG - chr1.1:58849526-58849545 MS.gene74858:CDS 25.0%
!! ATTCTTCAGAGTATTTATGT+TGG + chr1.1:58849072-58849091 None:intergenic 25.0%
!! ATTTACTATCTTTTTGTCTC+GGG - chr1.1:58848580-58848599 MS.gene74858:CDS 25.0%
!! TTAAGGTGTTTATCTAAACA+CGG - chr1.1:58849284-58849303 MS.gene74858:intron 25.0%
AGTTATTGAAACTATAGCAC+TGG + chr1.1:58849432-58849451 None:intergenic 30.0%
ATTGAACATGATTTAGGCTT+AGG + chr1.1:58850022-58850041 None:intergenic 30.0%
GAAGAAATGCTACATGTTAT+CGG - chr1.1:58848798-58848817 MS.gene74858:intron 30.0%
! AGGAGTTTGTTTATTGTATG+GGG - chr1.1:58848823-58848842 MS.gene74858:intron 30.0%
! GAGGAGTTTGTTTATTGTAT+GGG - chr1.1:58848822-58848841 MS.gene74858:intron 30.0%
! GATTTTTCTTAGCTAATTCG+TGG - chr1.1:58848726-58848745 MS.gene74858:CDS 30.0%
!! ATCAATCACATTAGTCTTGA+AGG + chr1.1:58849369-58849388 None:intergenic 30.0%
!! GCTGTTTTCATAAGTTATCA+TGG + chr1.1:58849178-58849197 None:intergenic 30.0%
AAGAATTTGTGAGATGGTGT+AGG - chr1.1:58849086-58849105 MS.gene74858:intron 35.0%
AAGCACTCGACTTAAATACA+AGG + chr1.1:58849640-58849659 None:intergenic 35.0%
ATAGGTGTTTATGAGGTCAA+AGG - chr1.1:58849950-58849969 MS.gene74858:CDS 35.0%
CTATCTCCAAAGACTTTGTA+CGG - chr1.1:58849608-58849627 MS.gene74858:intron 35.0%
GAGTTTCATGATTTACCTGA+AGG - chr1.1:58848313-58848332 MS.gene74858:CDS 35.0%
GTAAGATAGAGATCCTTCTT+AGG + chr1.1:58848523-58848542 None:intergenic 35.0%
GTGGAATTAGAGCTTTCAAT+TGG - chr1.1:58848762-58848781 MS.gene74858:CDS 35.0%
TCGTGGTTTATTGTGTTATG+TGG - chr1.1:58848743-58848762 MS.gene74858:CDS 35.0%
TTCAATTGGATAGAGCTGAT+GGG - chr1.1:58848776-58848795 MS.gene74858:CDS 35.0%
TTTCAATTGGATAGAGCTGA+TGG - chr1.1:58848775-58848794 MS.gene74858:CDS 35.0%
! ACTCTGAAGAATTTGTGAGA+TGG - chr1.1:58849080-58849099 MS.gene74858:intron 35.0%
! CGAGGAGTTTGTTTATTGTA+TGG - chr1.1:58848821-58848840 MS.gene74858:intron 35.0%
! GTGGTTGTTATTGAAGAAGA+AGG - chr1.1:58848286-58848305 MS.gene74858:CDS 35.0%
!! AAAAATCAGGTTTTCTGAGG+TGG - chr1.1:58849529-58849548 MS.gene74858:CDS 35.0%
!! CTTTCTATTGGTTCTACTAG+TGG + chr1.1:58849827-58849846 None:intergenic 35.0%
AAACCACTTCTCATAGACGA+TGG - chr1.1:58848547-58848566 MS.gene74858:CDS 40.0%
ACTGGTCAATCTAGTGACTA+AGG + chr1.1:58849414-58849433 None:intergenic 40.0%
ATGGCTGATACTCAAATCCA+TGG + chr1.1:58849670-58849689 None:intergenic 40.0%
CAATGAAGGAGGAGATGATA+AGG - chr1.1:58849919-58849938 MS.gene74858:CDS 40.0%
CCAAACTGAATCAGATTCAG+CGG + chr1.1:58848419-58848438 None:intergenic 40.0%
CGCTGAATCTGATTCAGTTT+GGG - chr1.1:58848417-58848436 MS.gene74858:CDS 40.0%
CTAAACCAGACACCTTCATT+AGG + chr1.1:58848612-58848631 None:intergenic 40.0%
CTGAACTTGTAAGTGTGTGT+TGG - chr1.1:58849552-58849571 MS.gene74858:intron 40.0%
GAGTGAATATCCCAGTAGTA+CGG + chr1.1:58849796-58849815 None:intergenic 40.0%
GATAGAGATCCTTCTTAGGA+AGG + chr1.1:58848519-58848538 None:intergenic 40.0%
GCGAAATGTATTCTTGGATG+CGG - chr1.1:58849730-58849749 MS.gene74858:intron 40.0%
GGATTACTGTTCCAGATTCA+AGG - chr1.1:58848869-58848888 MS.gene74858:intron 40.0%
GTAATCCATCTCCAATACCT+CGG + chr1.1:58848856-58848875 None:intergenic 40.0%
GTATTTAAGTCGAGTGCTTC+TGG - chr1.1:58849641-58849660 MS.gene74858:intron 40.0%
GTTATCTGTCCTTCCTAAGA+AGG - chr1.1:58848507-58848526 MS.gene74858:CDS 40.0%
GTTTATGAGGTCAAAGGTGA+TGG - chr1.1:58849956-58849975 MS.gene74858:CDS 40.0%
TATGCTCCGTACAAAGTCTT+TGG + chr1.1:58849617-58849636 None:intergenic 40.0%
TGGCTGATACTCAAATCCAT+GGG + chr1.1:58849669-58849688 None:intergenic 40.0%
TGTGGAGCGAAATGTATTCT+TGG - chr1.1:58849724-58849743 MS.gene74858:intron 40.0%
TTGGGAGAGTTCTTCAATGA+AGG - chr1.1:58849905-58849924 MS.gene74858:CDS 40.0%
! ACGTTTTCAATGCAACCTTC+AGG + chr1.1:58848331-58848350 None:intergenic 40.0%
!! AACATCATGTGGTGTTGTGA+AGG + chr1.1:58848359-58848378 None:intergenic 40.0%
!! TCAAACCTAATGAAGGTGTC+TGG - chr1.1:58848604-58848623 MS.gene74858:CDS 40.0%
AACTGAATCAGATTCAGCGG+CGG + chr1.1:58848416-58848435 None:intergenic 45.0%
AATGCTACATGTTATCGGCG+AGG - chr1.1:58848803-58848822 MS.gene74858:intron 45.0%
ATCCAAAGCAGAGAGCAGAT+GGG - chr1.1:58849867-58849886 MS.gene74858:CDS 45.0%
CCGCTGAATCTGATTCAGTT+TGG - chr1.1:58848416-58848435 MS.gene74858:CDS 45.0%
CTCGGGTTCAAACCTAATGA+AGG - chr1.1:58848597-58848616 MS.gene74858:CDS 45.0%
GATGATAAGGAGGTGGAGAT+AGG - chr1.1:58849932-58849951 MS.gene74858:CDS 45.0%
GGAGAGTTCTTCAATGAAGG+AGG - chr1.1:58849908-58849927 MS.gene74858:CDS 45.0%
GGTGGAGATAGGTGTTTATG+AGG - chr1.1:58849943-58849962 MS.gene74858:CDS 45.0%
GTATTCTTGGATGCGGAAAG+AGG - chr1.1:58849737-58849756 MS.gene74858:intron 45.0%
TATCCAAAGCAGAGAGCAGA+TGG - chr1.1:58849866-58849885 MS.gene74858:CDS 45.0%
TCTCATAGACGATGGCGAAA+AGG - chr1.1:58848555-58848574 MS.gene74858:CDS 45.0%
TGAAGGAGGAGATGATAAGG+AGG - chr1.1:58849922-58849941 MS.gene74858:CDS 45.0%
TGATGTTGCGAGACTCTCTT+TGG - chr1.1:58848372-58848391 MS.gene74858:CDS 45.0%
! ATGAGGTCAAAGGTGATGGT+TGG - chr1.1:58849960-58849979 MS.gene74858:CDS 45.0%
! GTCAAAGGTGATGGTTGGAA+GGG - chr1.1:58849965-58849984 MS.gene74858:CDS 45.0%
!! AAGGGTGGTCTTGTTGTTCA+AGG - chr1.1:58849983-58850002 MS.gene74858:CDS 45.0%
!! ATACTCCGAGGTATTGGAGA+TGG - chr1.1:58848848-58848867 MS.gene74858:intron 45.0%
!! GTGTGTGTTGGTTCGAAATC+CGG - chr1.1:58849564-58849583 MS.gene74858:intron 45.0%
!! TGTGTGTTGGTTCGAAATCC+GGG - chr1.1:58849565-58849584 MS.gene74858:intron 45.0%
AGGAGGAGATGATAAGGAGG+TGG - chr1.1:58849925-58849944 MS.gene74858:CDS 50.0%
ATCACCTCCAGCAATGGCAA+TGG + chr1.1:58849702-58849721 None:intergenic 50.0%
ATGGCAATGGAGGCTTCACA+TGG + chr1.1:58849689-58849708 None:intergenic 50.0%
CACAGTATCACCTCCAGCAA+TGG + chr1.1:58849708-58849727 None:intergenic 50.0%
CATTGCTGGAGGTGATACTG+TGG - chr1.1:58849706-58849725 MS.gene74858:intron 50.0%
GAGAGTCTCGCAACATCATG+TGG + chr1.1:58848370-58848389 None:intergenic 50.0%
GGGTGGTTGGAGATGGAATT+GGG - chr1.1:58849887-58849906 MS.gene74858:CDS 50.0%
TACGGAAGCGAGGAACAATC+TGG + chr1.1:58849778-58849797 None:intergenic 50.0%
TCGCCATCGTCTATGAGAAG+TGG + chr1.1:58848553-58848572 None:intergenic 50.0%
TGGGTGGTTGGAGATGGAAT+TGG - chr1.1:58849886-58849905 MS.gene74858:CDS 50.0%
TTCCTCGCTTCCGTACTACT+GGG - chr1.1:58849783-58849802 MS.gene74858:CDS 50.0%
TTGTATGGGGAGATACTCCG+AGG - chr1.1:58848836-58848855 MS.gene74858:intron 50.0%
! AAAGGTGATGGTTGGAAGGG+TGG - chr1.1:58849968-58849987 MS.gene74858:CDS 50.0%
! GGTCAAAGGTGATGGTTGGA+AGG - chr1.1:58849964-58849983 MS.gene74858:CDS 50.0%
!! GTGTGTTGGTTCGAAATCCG+GGG - chr1.1:58849566-58849585 MS.gene74858:intron 50.0%
!! TGATAGTGTTGATCCAGCCC+CGG + chr1.1:58849586-58849605 None:intergenic 50.0%
ACCTCCAGCAATGGCAATGG+AGG + chr1.1:58849699-58849718 None:intergenic 55.0%
AGCAGATGGGTGGTTGGAGA+TGG - chr1.1:58849880-58849899 MS.gene74858:CDS 55.0%
ATCCCAGTAGTACGGAAGCG+AGG + chr1.1:58849788-58849807 None:intergenic 55.0%
CAAAGCAGAGAGCAGATGGG+TGG - chr1.1:58849870-58849889 MS.gene74858:CDS 55.0%
CACCCATCTGCTCTCTGCTT+TGG + chr1.1:58849872-58849891 None:intergenic 55.0%
GAAGCCTCCATTGCCATTGC+TGG - chr1.1:58849692-58849711 MS.gene74858:intron 55.0%
GGGGAGATACTCCGAGGTAT+TGG - chr1.1:58848842-58848861 MS.gene74858:intron 55.0%
GTTCCTCGCTTCCGTACTAC+TGG - chr1.1:58849782-58849801 MS.gene74858:CDS 55.0%
TGGATGCGGAAAGAGGACGA+AGG - chr1.1:58849744-58849763 MS.gene74858:intron 55.0%
!! TCGAGTGCTTCTGGAACCCA+TGG - chr1.1:58849650-58849669 MS.gene74858:intron 55.0%
GCAGAGAGCAGATGGGTGGT+TGG - chr1.1:58849874-58849893 MS.gene74858:CDS 60.0%
GCCTCCATTGCCATTGCTGG+AGG - chr1.1:58849695-58849714 MS.gene74858:intron 60.0%
!! GTTGGTTCGAAATCCGGGGC+TGG - chr1.1:58849570-58849589 MS.gene74858:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 58848283 58850051 58848283 ID=MS.gene74858
chr1.1 mRNA 58848283 58850051 58848283 ID=MS.gene74858.t1;Parent=MS.gene74858
chr1.1 exon 58849758 58850051 58849758 ID=MS.gene74858.t1.exon1;Parent=MS.gene74858.t1
chr1.1 CDS 58849758 58850051 58849758 ID=cds.MS.gene74858.t1;Parent=MS.gene74858.t1
chr1.1 exon 58849444 58849562 58849444 ID=MS.gene74858.t1.exon2;Parent=MS.gene74858.t1
chr1.1 CDS 58849444 58849562 58849444 ID=cds.MS.gene74858.t1;Parent=MS.gene74858.t1
chr1.1 exon 58848283 58848796 58848283 ID=MS.gene74858.t1.exon3;Parent=MS.gene74858.t1
chr1.1 CDS 58848283 58848796 58848283 ID=cds.MS.gene74858.t1;Parent=MS.gene74858.t1
Gene Sequence

>MS.gene74858

ATGGTGGTTGTTATTGAAGAAGAAGGTGTTGAGTTTCATGATTTACCTGAAGGTTGCATTGAAAACGTTCTCTCCTTCACAACACCACATGATGTTGCGAGACTCTCTTTGGTTTCATCAACTTTCAAATCCGCCGCTGAATCTGATTCAGTTTGGGATAAATTCTTGACATCTGATTATCACTCAATTATCTCTCAATCTGATGCAGAAATCGATAATAACTCGTTATCTGTCCTTCCTAAGAAGGATCTCTATCTTACTCAGAAACCACTTCTCATAGACGATGGCGAAAAGGTAATTTACTATCTTTTTGTCTCGGGTTCAAACCTAATGAAGGTGTCTGGTTTAGCAATATCATCGTTGTCAGTTGAGTTAGTCTATACAGATGTGTCGTTTTTATTACCATTAATAGCGAAGATATGTTTAGGTGTGTATGTTAATGTGATTTTTCTTAGCTAATTCGTGGTTTATTGTGTTATGTGGAATTAGAGCTTTCAATTGGATAGAGCTGATGGGAAGAAATGCTACATGTTATCGGCGAGGAGTTTGTTTATTGTATGGGGAGATACTCCGAGGTATTGGAGATGGATTACTGTTCCAGATTCAAGGTAATAATCAAATTTTAATTAGTAAGAGTTAATCGTTGATTTTTTTTAAATTAAGAGTTAATCATTGATTTATTTTTGAAGTTGTAGATATTGGTTAATTTCAGTTGTGAAATTAAACGAAATTATGAAATGATTTATGAAATACGCATGTTTGGATTGACTTATTTGAGCTTGTCTACCAACATAAATACTCTGAAGAATTTGTGAGATGGTGTAGGATAGCTTATAATTTTTTTTTATGAAATATGCCCATAAGCTGTTTTCAGTTTATTTTCGTAAGTTCTCCATGATAACTTATGAAAACAGCTTATAGCTTAAATGAAAATTGTTTGATTCTATTTTATATTTGCTTTATATATAAGCATGTATGTTTATCATACAATAAACGTTTAATTAAGGTGTTTATCTAAACACGGCCTAAATTGATCCATGCTTATTAGTACATTAAGATCAATCAATTTAGTAAGAAAATTGTCCTTCAAGACTAATGTGATTGATGATTTTATTCTAGAGATTACTTCCTTAGTCACTAGATTGACCAGTGCTATAGTTTCAATAACTAAATTTGGTGATTTATTCTTTAATTTGTTACCTATATATTTCAATGGTGATTGAACAAAATCTAATCAATTCAAAAAAAAAATCAGGTTTTCTGAGGTGGCTGAACTTGTAAGTGTGTGTTGGTTCGAAATCCGGGGCTGGATCAACACTATCATGCTATCTCCAAAGACTTTGTACGGAGCATACCTTGTATTTAAGTCGAGTGCTTCTGGAACCCATGGATTTGAGTATCAGCCATGTGAAGCCTCCATTGCCATTGCTGGAGGTGATACTGTGGAGCGAAATGTATTCTTGGATGCGGAAAGAGGACGAAGGCTAAGATACCAGATTGTTCCTCGCTTCCGTACTACTGGGATATTCACTCGACTTCGACCACTAGTAGAACCAATAGAAAGTGTAGATGATATGCAGAAGTATCCAAAGCAGAGAGCAGATGGGTGGTTGGAGATGGAATTGGGAGAGTTCTTCAATGAAGGAGGAGATGATAAGGAGGTGGAGATAGGTGTTTATGAGGTCAAAGGTGATGGTTGGAAGGGTGGTCTTGTTGTTCAAGGAATTGAAATAAGGCCTAAGCCTAAATCATGTTCAATGAATGACTAA
Protein sequence

>MS.gene74858.t1

MVVVIEEEGVEFHDLPEGCIENVLSFTTPHDVARLSLVSSTFKSAAESDSVWDKFLTSDYHSIISQSDAEIDNNSLSVLPKKDLYLTQKPLLIDDGEKSFQLDRADGKKCYMLSARSLFIVWGDTPRYWRWITVPDSRFSEVAELVSVCWFEIRGWINTIMLSPKTLYGAYLVFKSSASGTHGFEYQPCEASIAIAGGDTVERNVFLDAERGRRLRYQIVPRFRTTGIFTRLRPLVEPIESVDDMQKYPKQRADGWLEMELGEFFNEGGDDKEVEIGVYEVKGDGWKGGLVVQGIEIRPKPKSCSMND