AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49088

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49088.t1 MTR_1g100597 93.907 279 13 2 1 279 1 275 0.0 531
MS.gene49088.t1 MTR_1g079370 73.188 276 63 1 1 276 1 265 6.46e-146 410
MS.gene49088.t1 MTR_1g079180 74.194 279 55 4 1 276 1 265 6.29e-144 405
MS.gene49088.t1 MTR_1g079260 70.423 284 70 3 1 279 1 275 2.53e-141 399
MS.gene49088.t1 MTR_1g079230 69.643 280 80 2 1 279 1 276 8.52e-140 395
MS.gene49088.t1 MTR_1g100613 80.417 240 47 0 20 259 5 244 1.41e-137 388
MS.gene49088.t1 MTR_1g079460 69.504 282 70 2 1 279 1 269 2.77e-137 388
MS.gene49088.t1 MTR_1g079200 70.819 281 60 4 1 276 1 264 4.26e-135 382
MS.gene49088.t1 MTR_1g079380 71.942 278 67 1 1 278 1 267 1.53e-133 380
MS.gene49088.t1 MTR_1g079850 69.014 284 83 3 1 279 1 284 6.26e-132 375
MS.gene49088.t1 MTR_3g006850 63.934 305 80 5 3 279 16 318 1.34e-122 353
MS.gene49088.t1 MTR_1g079250 65.734 286 86 6 1 279 1 281 2.50e-114 331
MS.gene49088.t1 MTR_1g026510 61.168 291 96 7 1 279 1 286 1.94e-112 326
MS.gene49088.t1 MTR_3g452600 61.594 276 91 2 4 279 10 270 6.70e-112 324
MS.gene49088.t1 MTR_3g450990 55.442 294 96 4 4 279 10 286 1.41e-99 293
MS.gene49088.t1 MTR_1g079410 55.396 278 105 4 5 276 9 273 5.67e-99 291
MS.gene49088.t1 MTR_2g079100 58.608 273 92 4 3 273 8 261 6.82e-98 288
MS.gene49088.t1 MTR_1g079350 54.355 287 108 6 1 276 1 275 1.08e-93 278
MS.gene49088.t1 MTR_1g079340 52.667 300 104 8 1 276 40 325 4.47e-85 258
MS.gene49088.t1 MTR_7g096300 58.397 262 83 5 1 256 1 242 1.85e-83 265
MS.gene49088.t1 MTR_1g079140 47.508 301 129 6 4 279 13 309 1.88e-83 253
MS.gene49088.t1 MTR_1g079400 47.872 282 122 3 5 276 13 279 1.34e-80 244
MS.gene49088.t1 MTR_1g078470 60.104 193 61 3 84 276 8 184 8.51e-74 224
MS.gene49088.t1 MTR_7g112550 44.291 289 137 10 2 276 5 283 7.72e-70 217
MS.gene49088.t1 MTR_1g083580 40.860 279 158 5 2 276 6 281 2.83e-65 206
MS.gene49088.t1 MTR_7g112560 39.711 277 145 7 4 277 1 258 3.78e-62 197
MS.gene49088.t1 MTR_1g079240 57.368 190 59 4 87 276 12 179 3.25e-61 192
MS.gene49088.t1 MTR_1g076870 70.149 134 35 2 128 256 2 135 1.97e-59 186
MS.gene49088.t1 MTR_4g023570 37.319 276 131 7 4 276 1 237 3.85e-52 171
MS.gene49088.t1 MTR_1g079210 66.667 123 40 1 129 250 7 129 6.89e-46 151
MS.gene49088.t1 MTR_1g079130 44.444 189 92 2 101 276 1 189 1.52e-44 149
MS.gene49088.t1 MTR_1g079350 60.584 137 41 3 1 128 1 133 2.22e-44 150
MS.gene49088.t1 MTR_2g042540 65.714 105 34 2 1 103 1 105 2.47e-37 129
MS.gene49088.t1 MTR_1g078460 71.264 87 25 0 1 87 64 150 8.35e-36 125
MS.gene49088.t1 MTR_7g096310 56.061 132 50 3 57 186 38 163 3.13e-35 125
MS.gene49088.t1 MTR_3g058640 54.867 113 38 1 128 240 7 106 4.07e-34 120
MS.gene49088.t1 MTR_7g103380 26.316 285 173 5 6 275 21 283 9.47e-30 114
MS.gene49088.t1 MTR_0113s0040 30.846 201 124 3 2 188 20 219 5.13e-24 98.6
MS.gene49088.t1 MTR_1g101390 38.621 145 86 2 2 144 26 169 6.03e-23 95.9
MS.gene49088.t1 MTR_1g079220 60.759 79 18 1 165 243 1 66 1.07e-22 89.4
MS.gene49088.t1 MTR_4g083970 26.818 220 126 4 63 277 102 291 1.84e-20 89.0
MS.gene49088.t1 MTR_0002s0020 30.539 167 106 3 6 165 24 187 1.04e-18 84.3
MS.gene49088.t1 MTR_4g102770 33.333 144 92 2 6 146 21 163 6.86e-18 82.0
MS.gene49088.t1 MTR_5g010800 30.137 146 95 4 6 147 19 161 7.84e-16 75.9
MS.gene49088.t1 MTR_1g079390 72.000 50 12 2 222 271 8 55 3.74e-15 68.6
MS.gene49088.t1 MTR_1g079190 73.171 41 11 0 228 268 134 174 2.38e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49088.t1 AT2G02360 42.909 275 134 5 4 275 14 268 2.03e-74 229
MS.gene49088.t1 AT1G80110 43.542 271 134 6 6 275 3 255 1.12e-67 211
MS.gene49088.t1 AT2G02240 42.336 274 138 5 4 275 61 316 1.33e-64 205
MS.gene49088.t1 AT2G02340 42.446 278 142 5 4 275 36 301 1.87e-64 204
MS.gene49088.t1 AT2G02230 37.540 309 156 7 4 279 32 336 3.30e-63 202
MS.gene49088.t1 AT2G02250 41.667 276 138 8 4 275 47 303 4.97e-63 201
MS.gene49088.t1 AT1G09155 41.935 279 152 6 7 276 3 280 6.84e-63 200
MS.gene49088.t1 AT1G56250 41.429 280 154 5 7 277 4 282 9.68e-61 194
MS.gene49088.t1 AT1G56240 41.007 278 154 5 7 275 4 280 3.05e-59 190
MS.gene49088.t1 AT2G02350 37.410 278 153 7 2 275 22 282 9.51e-57 184
MS.gene49088.t1 AT2G02310 35.507 276 159 4 4 276 45 304 1.13e-52 174
MS.gene49088.t1 AT2G02320 37.545 277 146 7 6 275 42 298 3.19e-49 165
MS.gene49088.t1 AT2G02300 34.875 281 142 9 4 276 34 281 2.48e-42 147
MS.gene49088.t1 AT5G24560 42.188 192 98 4 4 192 3 184 2.83e-42 144
MS.gene49088.t1 AT3G53000 28.179 291 171 7 3 278 20 287 3.14e-30 115
MS.gene49088.t1 AT2G02350 33.520 179 101 6 101 275 1 165 2.70e-26 102
MS.gene49088.t1 AT1G12710 25.517 290 164 7 3 275 27 281 2.10e-20 89.0
MS.gene49088.t1 AT1G63090 30.097 206 125 6 3 192 25 227 2.57e-19 85.9
MS.gene49088.t1 AT5G52120 24.749 299 165 8 1 275 18 280 1.57e-18 84.0
MS.gene49088.t1 AT3G61060 31.915 141 93 2 6 144 27 166 3.66e-18 82.8
MS.gene49088.t1 AT3G61060 30.496 141 96 1 6 144 27 167 2.81e-17 80.1
MS.gene49088.t1 AT4G19840 29.319 191 102 7 89 276 85 245 4.40e-15 73.6
MS.gene49088.t1 AT1G12710 25.581 301 192 8 3 275 27 323 1.91e-14 72.8

Find 80 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGCTGCATACTGAGTATT+TGG 0.255011 1.3:+69361620 None:intergenic
TTCTGCCTGCGTTCTACATT+TGG 0.271945 1.3:+69361491 None:intergenic
CTTGTATACAAATTGATTGA+TGG 0.301820 1.3:-69361596 MS.gene49088:CDS
ATGGGAGAGTTCTTCAATTC+AGG 0.321358 1.3:-69361368 MS.gene49088:CDS
CATCATCGACGATGCCAAAA+AGG 0.324659 1.3:-69363503 MS.gene49088:intron
CTAGATCTCTCGCTATTACT+TGG 0.338529 1.3:-69362539 MS.gene49088:CDS
CGCAGACATGGAAGACTTTA+CGG 0.338884 1.3:-69361467 MS.gene49088:CDS
AGCAAATGCTCCTTCCAAAA+AGG 0.353184 1.3:-69363557 MS.gene49088:CDS
TAGCACTAAAATTGTCTGTT+TGG 0.362941 1.3:-69361516 MS.gene49088:CDS
GATGAGTGTTATCGAGATAA+AGG 0.385187 1.3:-69361324 MS.gene49088:CDS
AGACGAAGATTAGCAACTTC+GGG 0.388577 1.3:+69361692 None:intergenic
TGAAATGCAGAGCTTTCAAT+TGG 0.399907 1.3:-69362598 MS.gene49088:intron
GGACCACTATGCCTGATTCC+AGG 0.401514 1.3:-69362491 MS.gene49088:intron
CCGCCGATTCCGATGCCGTC+TGG 0.403257 1.3:-69363633 MS.gene49088:CDS
TAGATCTCTCGCTATTACTT+GGG 0.415079 1.3:-69362538 MS.gene49088:CDS
GAGAGTCTGCCGGCGTCAAC+AGG 0.416723 1.3:+69363682 None:intergenic
AGGAGTCGTACGAGACAGTA+TGG 0.422103 1.3:+69363702 None:intergenic
AAGGGGAATTTCTTTCTTGA+GGG 0.424911 1.3:-69361290 MS.gene49088:CDS
AAAATGGCCAACCTGGAATC+AGG 0.426615 1.3:+69362480 None:intergenic
TGATGATAGGTCGATCAGAT+AGG 0.426684 1.3:+69363521 None:intergenic
TTATCTCGATAACACTCATC+TGG 0.440534 1.3:+69361327 None:intergenic
ATTCCAGACGGCATCGGAAT+CGG 0.451722 1.3:+69363630 None:intergenic
GTTGCTTAATTGTCTACCTT+AGG 0.451895 1.3:+69361254 None:intergenic
CGCAGGCAGAACCGCAGACA+TGG 0.453913 1.3:-69361479 MS.gene49088:CDS
ATGATGACCGCTATTGGATC+TGG 0.457353 1.3:-69362512 MS.gene49088:CDS
TTGGCATCGTCGATGATGAT+AGG 0.465612 1.3:+69363508 None:intergenic
CAAGGGGAATTTCTTTCTTG+AGG 0.468108 1.3:-69361291 MS.gene49088:CDS
TACAAATTGATTGATGGCTA+TGG 0.469002 1.3:-69361590 MS.gene49088:CDS
GGGGTGATGATGACCGCTAT+TGG 0.473829 1.3:-69362518 MS.gene49088:CDS
ACAAATTGATTGATGGCTAT+GGG 0.489453 1.3:-69361589 MS.gene49088:CDS
CGTCCTTGTGTGAGAAGTGA+TGG 0.489686 1.3:-69361407 MS.gene49088:CDS
CTCAAGAAAGAAATTCCCCT+TGG 0.498320 1.3:+69361292 None:intergenic
ATGATAGAGTCGATGTCAGA+AGG 0.501608 1.3:+69363598 None:intergenic
AAGACGAAGATTAGCAACTT+CGG 0.509597 1.3:+69361691 None:intergenic
CTTCCTGTGGATTTATCTGT+CGG 0.516064 1.3:-69361557 MS.gene49088:CDS
GGCGGCAGAAAGGAAAGTCT+TGG 0.517109 1.3:+69363651 None:intergenic
TGATAGAGTCGATGTCAGAA+GGG 0.519983 1.3:+69363599 None:intergenic
GGGTTTGAAGATTTAGCAGA+AGG 0.520179 1.3:-69363736 MS.gene49088:CDS
GGGTGGTTGGAGATTGAGAT+GGG 0.540728 1.3:-69361386 MS.gene49088:CDS
CATCGGAATCGGCGGCAGAA+AGG 0.542303 1.3:+69363641 None:intergenic
TGGGTGGTTGGAGATTGAGA+TGG 0.542699 1.3:-69361387 MS.gene49088:CDS
GTGTGGTGGCTCGAAATTCG+TGG 0.547967 1.3:-69361668 MS.gene49088:CDS
TGGGTTTGAGAACCTTCCTG+TGG 0.549562 1.3:-69361570 MS.gene49088:CDS
GATGATAGGTCGATCAGATA+GGG 0.551413 1.3:+69363522 None:intergenic
TGTGAGAAGTGATGGGTGGT+TGG 0.556044 1.3:-69361399 MS.gene49088:CDS
GATTTATCTGTCGGTGTCGA+AGG 0.557631 1.3:-69361548 MS.gene49088:CDS
CTAATCTTCGTCTTGTGTGG+TGG 0.562220 1.3:-69361682 MS.gene49088:CDS
TTGCTAATCTTCGTCTTGTG+TGG 0.565369 1.3:-69361685 MS.gene49088:CDS
AGTGTTATCGAGATAAAGGC+TGG 0.568289 1.3:-69361320 MS.gene49088:CDS
GGAAACGACGGAGAGTCTGC+CGG 0.570360 1.3:+69363672 None:intergenic
GAAGGAGCATTTGCTAGAGA+AGG 0.571483 1.3:+69363565 None:intergenic
CAACCTGGAATCAGGCATAG+TGG 0.580151 1.3:+69362488 None:intergenic
GAAGACTTTACGGTCGTGCG+AGG 0.580931 1.3:-69361457 MS.gene49088:CDS
GGGAATAGAAGTGAGGCCTA+AGG 0.588625 1.3:-69361270 MS.gene49088:CDS
AGGTTGCATGACAGAGTGGT+AGG 0.597534 1.3:-69361437 MS.gene49088:CDS
TTATCTGTCGGTGTCGAAGG+TGG 0.597798 1.3:-69361545 MS.gene49088:CDS
CACCCATCACTTCTCACACA+AGG 0.598274 1.3:+69361404 None:intergenic
ATAAAGGCTGGTGAGACCAA+GGG 0.599634 1.3:-69361308 MS.gene49088:CDS
GATAAAGGCTGGTGAGACCA+AGG 0.599999 1.3:-69361309 MS.gene49088:CDS
TTCTTGAGGGAATAGAAGTG+AGG 0.602948 1.3:-69361277 MS.gene49088:CDS
TGTGGTGGCTCGAAATTCGT+GGG 0.618223 1.3:-69361667 MS.gene49088:CDS
AGATCTCTCGCTATTACTTG+GGG 0.621524 1.3:-69362537 MS.gene49088:CDS
CCAGACGGCATCGGAATCGG+CGG 0.622067 1.3:+69363633 None:intergenic
GTCCTTGTGTGAGAAGTGAT+GGG 0.633145 1.3:-69361406 MS.gene49088:CDS
ACACCGACAGATAAATCCAC+AGG 0.642651 1.3:+69361554 None:intergenic
ATACTGAGTATTTGGAGACA+AGG 0.643026 1.3:+69361628 None:intergenic
AAAGTGATTCCAGACGGCAT+CGG 0.647589 1.3:+69363624 None:intergenic
TAAAGGCTGGTGAGACCAAG+GGG 0.647680 1.3:-69361307 MS.gene49088:CDS
TCACTTCTCACACAAGGACG+CGG 0.648694 1.3:+69361410 None:intergenic
TGGATCCAAATGTAGAACGC+AGG 0.654508 1.3:-69361496 MS.gene49088:CDS
AAGGAAAGTCTTGGAAACGA+CGG 0.661166 1.3:+69363660 None:intergenic
CGTAAAGTCTTCCATGTCTG+CGG 0.661378 1.3:+69361468 None:intergenic
AGGGAGAAAGTGATTCCAGA+CGG 0.665074 1.3:+69363618 None:intergenic
TGCGAGGTTGCATGACAGAG+TGG 0.676261 1.3:-69361441 MS.gene49088:CDS
CGACAGATAAATCCACAGGA+AGG 0.695590 1.3:+69361558 None:intergenic
GACGAAGATTAGCAACTTCG+GGG 0.697062 1.3:+69361693 None:intergenic
CGTACGACTCCTGTTGACGC+CGG 0.716965 1.3:-69363691 MS.gene49088:CDS
CTTGTGTGAGAAGTGATGGG+TGG 0.722396 1.3:-69361403 MS.gene49088:CDS
TAGTGGTCCAGATCCAATAG+CGG 0.737188 1.3:+69362505 None:intergenic
AGTCGTACGAGACAGTATGG+AGG 0.760723 1.3:+69363705 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAATTATTTAAAAAAGAAA+TGG + chr1.3:69361901-69361920 None:intergenic 10.0%
!! TACATTTATTAGTATTATTA+GGG - chr1.3:69361712-69361731 MS.gene49088:intron 10.0%
!! AAAAAGTAAAACAAAAAAAG+AGG - chr1.3:69362237-69362256 MS.gene49088:intron 15.0%
!! AATAAAAATGAGTAAATTTG+GGG + chr1.3:69362551-69362570 None:intergenic 15.0%
!! CAATAAAAATGAGTAAATTT+GGG + chr1.3:69362552-69362571 None:intergenic 15.0%
!! GTACATTTATTAGTATTATT+AGG - chr1.3:69361711-69361730 MS.gene49088:intron 15.0%
!! TGAAATAATTTAGTTATCAT+TGG - chr1.3:69363036-69363055 MS.gene49088:intron 15.0%
!! AAAAAAAAGTTATTAGACAG+TGG + chr1.3:69362959-69362978 None:intergenic 20.0%
!! ATTGATTGCTGTAAAATATA+TGG - chr1.3:69362134-69362153 MS.gene49088:intron 20.0%
!! CCAATAAAAATGAGTAAATT+TGG + chr1.3:69362553-69362572 None:intergenic 20.0%
!!! ACTGATTTTGATTAATTTGT+AGG + chr1.3:69362012-69362031 None:intergenic 20.0%
!!! CCAAATTTACTCATTTTTAT+TGG - chr1.3:69362550-69362569 MS.gene49088:CDS 20.0%
!!! CTGATTTTGATTAATTTGTA+GGG + chr1.3:69362011-69362030 None:intergenic 20.0%
!!! TGATTTTGATTAATTTGTAG+GGG + chr1.3:69362010-69362029 None:intergenic 20.0%
! ATTGATTGTTCTTTCATGTT+AGG - chr1.3:69361588-69361607 MS.gene49088:CDS 25.0%
! CTTGTATACAAATTGATTGA+TGG - chr1.3:69363405-69363424 MS.gene49088:intron 25.0%
! GAACAATGTTAAAACAAACA+AGG + chr1.3:69362591-69362610 None:intergenic 25.0%
! GAATTGAGAAAAAGTTGAAA+AGG + chr1.3:69363249-69363268 None:intergenic 25.0%
! TGAGAAGATATAGAAAATAC+AGG + chr1.3:69362114-69362133 None:intergenic 25.0%
! TGCCATAATAAAAGAAATAG+CGG - chr1.3:69362283-69362302 MS.gene49088:intron 25.0%
! TTGATTGTTCTTTCATGTTA+GGG - chr1.3:69361589-69361608 MS.gene49088:CDS 25.0%
! TTTCAATTGGATAGAAAAAG+TGG - chr1.3:69362416-69362435 MS.gene49088:intron 25.0%
!! AATATGTGATGATACCTTTT+TGG + chr1.3:69361515-69361534 None:intergenic 25.0%
!! AATGCTAATAATAAGCCTTT+TGG - chr1.3:69362374-69362393 MS.gene49088:intron 25.0%
!! ATGCTAATAATAAGCCTTTT+GGG - chr1.3:69362375-69362394 MS.gene49088:intron 25.0%
!! GTTATTACTCTTTTTGATGT+TGG - chr1.3:69363130-69363149 MS.gene49088:intron 25.0%
!!! AACCGCTATTTCTTTTATTA+TGG + chr1.3:69362288-69362307 None:intergenic 25.0%
!!! CCTTTTTTAAATGCTTAGTT+CGG - chr1.3:69362351-69362370 MS.gene49088:intron 25.0%
AAAAATGAGTAAATTTGGGG+AGG + chr1.3:69362548-69362567 None:intergenic 30.0%
AATTGTTCATTCCATTTCCT+TGG - chr1.3:69362628-69362647 MS.gene49088:intron 30.0%
AGATGAAAGTTTCCTTAATG+AGG + chr1.3:69362695-69362714 None:intergenic 30.0%
ATACGAAATATCCAAGGAAA+TGG + chr1.3:69362642-69362661 None:intergenic 30.0%
ATGAAAGTTTCCTTAATGAG+GGG + chr1.3:69362693-69362712 None:intergenic 30.0%
CCGAACTAAGCATTTAAAAA+AGG + chr1.3:69362354-69362373 None:intergenic 30.0%
GATGAAAGTTTCCTTAATGA+GGG + chr1.3:69362694-69362713 None:intergenic 30.0%
GTTAAAACAAACAAGGTAGA+AGG + chr1.3:69362584-69362603 None:intergenic 30.0%
TAGCACTAAAATTGTCTGTT+TGG - chr1.3:69363485-69363504 MS.gene49088:intron 30.0%
TCAGCCAATATACATTTACA+GGG + chr1.3:69362073-69362092 None:intergenic 30.0%
TGCATTTCAATATACCCAAA+AGG + chr1.3:69362392-69362411 None:intergenic 30.0%
TTCAGCCAATATACATTTAC+AGG + chr1.3:69362074-69362093 None:intergenic 30.0%
TTGAATATACCCATAGTCTT+TGG - chr1.3:69362740-69362759 MS.gene49088:intron 30.0%
! ACAAATTGATTGATGGCTAT+GGG - chr1.3:69363412-69363431 MS.gene49088:intron 30.0%
! TACAAATTGATTGATGGCTA+TGG - chr1.3:69363411-69363430 MS.gene49088:intron 30.0%
! TCATCATCGTCTGAAATATT+AGG - chr1.3:69361535-69361554 MS.gene49088:CDS 30.0%
!! ACAAGAAGTATTCACTTAGA+AGG + chr1.3:69362871-69362890 None:intergenic 30.0%
!! AGACAATTTTAGTGCTACTA+TGG + chr1.3:69363482-69363501 None:intergenic 30.0%
!!! ACTGCATTTTGTTATTCTTG+AGG + chr1.3:69362210-69362229 None:intergenic 30.0%
!!! GAGCTGTTTCAAAGTTTTTT+TGG - chr1.3:69363092-69363111 MS.gene49088:intron 30.0%
AAAACAAACAAGGTAGAAGG+TGG + chr1.3:69362581-69362600 None:intergenic 35.0%
AACCTCAATAACAGTGATGT+TGG - chr1.3:69362932-69362951 MS.gene49088:intron 35.0%
AAGACGAAGATTAGCAACTT+CGG + chr1.3:69363313-69363332 None:intergenic 35.0%
AAGGGGAATTTCTTTCTTGA+GGG - chr1.3:69363711-69363730 MS.gene49088:CDS 35.0%
AATGAACAATTCACCGAGAA+AGG + chr1.3:69362619-69362638 None:intergenic 35.0%
ACTCAGATACGAAATATCCA+AGG + chr1.3:69362648-69362667 None:intergenic 35.0%
AGAAAATACAGGCATAGTGT+CGG + chr1.3:69362103-69362122 None:intergenic 35.0%
AGAAGAAAAGCCAAAGACTA+TGG + chr1.3:69362753-69362772 None:intergenic 35.0%
ATACTGAGTATTTGGAGACA+AGG + chr1.3:69363376-69363395 None:intergenic 35.0%
CATTGTTCTTTCTCCTTTCT+CGG - chr1.3:69362603-69362622 MS.gene49088:intron 35.0%
CTTTACAAACCAAACCAACA+TGG + chr1.3:69361770-69361789 None:intergenic 35.0%
GAAGAAAAGCCAAAGACTAT+GGG + chr1.3:69362752-69362771 None:intergenic 35.0%
GATGAGTGTTATCGAGATAA+AGG - chr1.3:69363677-69363696 MS.gene49088:CDS 35.0%
GTAAATTTGGGGAGGAAAAA+TGG + chr1.3:69362540-69362559 None:intergenic 35.0%
TAAGCTGCATACTGAGTATT+TGG + chr1.3:69363384-69363403 None:intergenic 35.0%
TAGATCTCTCGCTATTACTT+GGG - chr1.3:69362463-69362482 MS.gene49088:intron 35.0%
TCAGGTGTATGGTTAACTTT+GGG + chr1.3:69362907-69362926 None:intergenic 35.0%
TGAAATGCAGAGCTTTCAAT+TGG - chr1.3:69362403-69362422 MS.gene49088:intron 35.0%
TGATCATATTCCCCTCATTA+AGG - chr1.3:69362680-69362699 MS.gene49088:intron 35.0%
TGTGCCCTGTAAATGTATAT+TGG - chr1.3:69362066-69362085 MS.gene49088:intron 35.0%
TGTTATTGAGGTTCAGATTC+AGG + chr1.3:69362925-69362944 None:intergenic 35.0%
TGTTCATAAGTGTAAGTGCT+AGG + chr1.3:69361814-69361833 None:intergenic 35.0%
TTATCTCGATAACACTCATC+TGG + chr1.3:69363677-69363696 None:intergenic 35.0%
TTCAGGTGTATGGTTAACTT+TGG + chr1.3:69362908-69362927 None:intergenic 35.0%
! TCTGGACTTCTTCATTTTCA+AGG + chr1.3:69363659-69363678 None:intergenic 35.0%
! TTGAATGTAACCAAGAGTGT+CGG + chr1.3:69361873-69361892 None:intergenic 35.0%
AAACCAACATGGTGGTTCAA+CGG + chr1.3:69361759-69361778 None:intergenic 40.0%
AACCCACATGTTTGGTAAGT+GGG + chr1.3:69362264-69362283 None:intergenic 40.0%
AAGGAAAGTCTTGGAAACGA+CGG + chr1.3:69361344-69361363 None:intergenic 40.0%
ACAATTACCGAAAACGTGTC+TGG - chr1.3:69361831-69361850 MS.gene49088:intron 40.0%
AGACGAAGATTAGCAACTTC+GGG + chr1.3:69363312-69363331 None:intergenic 40.0%
AGATCTCTCGCTATTACTTG+GGG - chr1.3:69362464-69362483 MS.gene49088:intron 40.0%
AGCAAATGCTCCTTCCAAAA+AGG - chr1.3:69361444-69361463 MS.gene49088:CDS 40.0%
AGTGTTATCGAGATAAAGGC+TGG - chr1.3:69363681-69363700 MS.gene49088:CDS 40.0%
ATGATAGAGTCGATGTCAGA+AGG + chr1.3:69361406-69361425 None:intergenic 40.0%
ATGGGAGAGTTCTTCAATTC+AGG - chr1.3:69363633-69363652 MS.gene49088:CDS 40.0%
ATTATGGCAACCCACATGTT+TGG + chr1.3:69362272-69362291 None:intergenic 40.0%
CAAGGGGAATTTCTTTCTTG+AGG - chr1.3:69363710-69363729 MS.gene49088:CDS 40.0%
CTAGATCTCTCGCTATTACT+TGG - chr1.3:69362462-69362481 MS.gene49088:intron 40.0%
CTCAAGAAAGAAATTCCCCT+TGG + chr1.3:69363712-69363731 None:intergenic 40.0%
CTTCCTGTGGATTTATCTGT+CGG - chr1.3:69363444-69363463 MS.gene49088:intron 40.0%
GACACACACAAACACAAACA+TGG + chr1.3:69361944-69361963 None:intergenic 40.0%
GACCAACATCACTGTTATTG+AGG + chr1.3:69362937-69362956 None:intergenic 40.0%
GATGATAGGTCGATCAGATA+GGG + chr1.3:69361482-69361501 None:intergenic 40.0%
GGATAGTTACACATTGACAG+CGG + chr1.3:69361923-69361942 None:intergenic 40.0%
GGGTTTGAAGATTTAGCAGA+AGG - chr1.3:69361265-69361284 MS.gene49088:CDS 40.0%
TACAAACCAAACCAACATGG+TGG + chr1.3:69361767-69361786 None:intergenic 40.0%
TGATAGAGTCGATGTCAGAA+GGG + chr1.3:69361405-69361424 None:intergenic 40.0%
TGATGATAGGTCGATCAGAT+AGG + chr1.3:69361483-69361502 None:intergenic 40.0%
TTCTTGAGGGAATAGAAGTG+AGG - chr1.3:69363724-69363743 MS.gene49088:CDS 40.0%
TTGCTAATCTTCGTCTTGTG+TGG - chr1.3:69363316-69363335 MS.gene49088:intron 40.0%
! AGATAGAGAGCCTTTTTGGA+AGG + chr1.3:69361457-69361476 None:intergenic 40.0%
AAAATGGCCAACCTGGAATC+AGG + chr1.3:69362524-69362543 None:intergenic 45.0%
AAAGTGATTCCAGACGGCAT+CGG + chr1.3:69361380-69361399 None:intergenic 45.0%
ACACCGACAGATAAATCCAC+AGG + chr1.3:69363450-69363469 None:intergenic 45.0%
AGGGAGAAAGTGATTCCAGA+CGG + chr1.3:69361386-69361405 None:intergenic 45.0%
ATAAAGGCTGGTGAGACCAA+GGG - chr1.3:69363693-69363712 MS.gene49088:CDS 45.0%
ATGATGACCGCTATTGGATC+TGG - chr1.3:69362489-69362508 MS.gene49088:intron 45.0%
ATGGTGGTTCAACGGAACAA+CGG + chr1.3:69361751-69361770 None:intergenic 45.0%
CAACCCACATGTTTGGTAAG+TGG + chr1.3:69362265-69362284 None:intergenic 45.0%
CATCATCGACGATGCCAAAA+AGG - chr1.3:69361498-69361517 MS.gene49088:CDS 45.0%
CATCTCTGCAACTTCTGTGT+TGG - chr1.3:69363278-69363297 MS.gene49088:intron 45.0%
CGACAGATAAATCCACAGGA+AGG + chr1.3:69363446-69363465 None:intergenic 45.0%
CGCAGACATGGAAGACTTTA+CGG - chr1.3:69363534-69363553 MS.gene49088:CDS 45.0%
CGTAAAGTCTTCCATGTCTG+CGG + chr1.3:69363536-69363555 None:intergenic 45.0%
CTAATCTTCGTCTTGTGTGG+TGG - chr1.3:69363319-69363338 MS.gene49088:intron 45.0%
GACGAAGATTAGCAACTTCG+GGG + chr1.3:69363311-69363330 None:intergenic 45.0%
GAGGTTCAGATTCAGGTGTA+TGG + chr1.3:69362918-69362937 None:intergenic 45.0%
GCAAGATGAACGACAATGAG+AGG - chr1.3:69362717-69362736 MS.gene49088:intron 45.0%
GTCCCACTTACCAAACATGT+GGG - chr1.3:69362259-69362278 MS.gene49088:intron 45.0%
GTCCTTGTGTGAGAAGTGAT+GGG - chr1.3:69363595-69363614 MS.gene49088:CDS 45.0%
GTGTTTGTGTGTGTCTGTGT+CGG - chr1.3:69361949-69361968 MS.gene49088:intron 45.0%
TAGTGGTCCAGATCCAATAG+CGG + chr1.3:69362499-69362518 None:intergenic 45.0%
TGGATCCAAATGTAGAACGC+AGG - chr1.3:69363505-69363524 MS.gene49088:CDS 45.0%
TTCTGCCTGCGTTCTACATT+TGG + chr1.3:69363513-69363532 None:intergenic 45.0%
TTGGCATCGTCGATGATGAT+AGG + chr1.3:69361496-69361515 None:intergenic 45.0%
! GGCAAGATAGAGAGCCTTTT+TGG + chr1.3:69361461-69361480 None:intergenic 45.0%
! GTTGAACCACCATGTTGGTT+TGG - chr1.3:69361758-69361777 MS.gene49088:intron 45.0%
!! GAAGGAGCATTTGCTAGAGA+AGG + chr1.3:69361439-69361458 None:intergenic 45.0%
!! GATTTATCTGTCGGTGTCGA+AGG - chr1.3:69363453-69363472 MS.gene49088:intron 45.0%
!! CATTAAATATATTATTAAAT+AGG - chr1.3:69361982-69362001 MS.gene49088:intron 5.0%
ACTGTTGTCACCGACACTCT+TGG - chr1.3:69361860-69361879 MS.gene49088:intron 50.0%
AGGAGTCGTACGAGACAGTA+TGG + chr1.3:69361302-69361321 None:intergenic 50.0%
AGGTTGCATGACAGAGTGGT+AGG - chr1.3:69363564-69363583 MS.gene49088:CDS 50.0%
AGTCGTACGAGACAGTATGG+AGG + chr1.3:69361299-69361318 None:intergenic 50.0%
CAACCTGGAATCAGGCATAG+TGG + chr1.3:69362516-69362535 None:intergenic 50.0%
CACCCATCACTTCTCACACA+AGG + chr1.3:69363600-69363619 None:intergenic 50.0%
CACTATGCCTGATTCCAGGT+TGG - chr1.3:69362514-69362533 MS.gene49088:CDS 50.0%
CGTCCTTGTGTGAGAAGTGA+TGG - chr1.3:69363594-69363613 MS.gene49088:CDS 50.0%
CTGCAACTTCTGTGTTGGAC+AGG - chr1.3:69363283-69363302 MS.gene49088:intron 50.0%
GATAAAGGCTGGTGAGACCA+AGG - chr1.3:69363692-69363711 MS.gene49088:CDS 50.0%
GGGAATAGAAGTGAGGCCTA+AGG - chr1.3:69363731-69363750 MS.gene49088:CDS 50.0%
GGGTGGTTGGAGATTGAGAT+GGG - chr1.3:69363615-69363634 MS.gene49088:CDS 50.0%
GGTCCCACTTACCAAACATG+TGG - chr1.3:69362258-69362277 MS.gene49088:intron 50.0%
GTTCCGTTGAACCACCATGT+TGG - chr1.3:69361753-69361772 MS.gene49088:intron 50.0%
TAAAGGCTGGTGAGACCAAG+GGG - chr1.3:69363694-69363713 MS.gene49088:CDS 50.0%
TCACTTCTCACACAAGGACG+CGG + chr1.3:69363594-69363613 None:intergenic 50.0%
TGGGTGGTTGGAGATTGAGA+TGG - chr1.3:69363614-69363633 MS.gene49088:CDS 50.0%
TGGGTTTGAGAACCTTCCTG+TGG - chr1.3:69363431-69363450 MS.gene49088:intron 50.0%
TGTGGTGGCTCGAAATTCGT+GGG - chr1.3:69363334-69363353 MS.gene49088:intron 50.0%
! ATTCCAGACGGCATCGGAAT+CGG + chr1.3:69361374-69361393 None:intergenic 50.0%
! CTTGTGTGAGAAGTGATGGG+TGG - chr1.3:69363598-69363617 MS.gene49088:CDS 50.0%
! TCGGACACCAGACACGTTTT+CGG + chr1.3:69361841-69361860 None:intergenic 50.0%
!! AGAGTGTCGGTGACAACAGT+CGG + chr1.3:69361860-69361879 None:intergenic 50.0%
!! TGTGAGAAGTGATGGGTGGT+TGG - chr1.3:69363602-69363621 MS.gene49088:CDS 50.0%
!! TTATCTGTCGGTGTCGAAGG+TGG - chr1.3:69363456-69363475 MS.gene49088:intron 50.0%
GAAGACTTTACGGTCGTGCG+AGG - chr1.3:69363544-69363563 MS.gene49088:CDS 55.0%
GGACCACTATGCCTGATTCC+AGG - chr1.3:69362510-69362529 MS.gene49088:CDS 55.0%
GGCGGCAGAAAGGAAAGTCT+TGG + chr1.3:69361353-69361372 None:intergenic 55.0%
GGGAGGAAAAATGGCCAACC+TGG + chr1.3:69362531-69362550 None:intergenic 55.0%
GTGTGGTGGCTCGAAATTCG+TGG - chr1.3:69363333-69363352 MS.gene49088:intron 55.0%
TGCGAGGTTGCATGACAGAG+TGG - chr1.3:69363560-69363579 MS.gene49088:CDS 55.0%
! GGGGTGATGATGACCGCTAT+TGG - chr1.3:69362483-69362502 MS.gene49088:intron 55.0%
CATCGGAATCGGCGGCAGAA+AGG + chr1.3:69361363-69361382 None:intergenic 60.0%
CGTACGACTCCTGTTGACGC+CGG - chr1.3:69361310-69361329 MS.gene49088:CDS 60.0%
GGAAACGACGGAGAGTCTGC+CGG + chr1.3:69361332-69361351 None:intergenic 60.0%
CGCAGGCAGAACCGCAGACA+TGG - chr1.3:69363522-69363541 MS.gene49088:CDS 65.0%
GAGAGTCTGCCGGCGTCAAC+AGG + chr1.3:69361322-69361341 None:intergenic 65.0%
! CCAGACGGCATCGGAATCGG+CGG + chr1.3:69361371-69361390 None:intergenic 65.0%
!! CCGCCGATTCCGATGCCGTC+TGG - chr1.3:69361368-69361387 MS.gene49088:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 69361259 69363764 69361259 ID=MS.gene49088
chr1.3 mRNA 69361259 69363764 69361259 ID=MS.gene49088.t1;Parent=MS.gene49088
chr1.3 exon 69363504 69363764 69363504 ID=MS.gene49088.t1.exon1;Parent=MS.gene49088.t1
chr1.3 CDS 69363504 69363764 69363504 ID=cds.MS.gene49088.t1;Parent=MS.gene49088.t1
chr1.3 exon 69362492 69362610 69362492 ID=MS.gene49088.t1.exon2;Parent=MS.gene49088.t1
chr1.3 CDS 69362492 69362610 69362492 ID=cds.MS.gene49088.t1;Parent=MS.gene49088.t1
chr1.3 exon 69361259 69361718 69361259 ID=MS.gene49088.t1.exon3;Parent=MS.gene49088.t1
chr1.3 CDS 69361259 69361718 69361259 ID=cds.MS.gene49088.t1;Parent=MS.gene49088.t1
Gene Sequence

>MS.gene49088

ATGACAGGGTTTGAAGATTTAGCAGAAGGATGCATTTCCTCCATACTGTCTCGTACGACTCCTGTTGACGCCGGCAGACTCTCCGTCGTTTCCAAGACTTTCCTTTCTGCCGCCGATTCCGATGCCGTCTGGAATCACTTTCTCCCTTCTGACATCGACTCTATCATCTCACAGTCACCTTCTCTAGCAAATGCTCCTTCCAAAAAGGCTCTCTATCTTGCCCTATCTGATCGACCTATCATCATCGACGATGCCAAAAAGGTATCATCACATATTTCATCATCGTCTGAAATATTAGGCTTTATTATCGTTGATCATACGTAAACTTAATTGATTGTTCTTTCATGTTAGGGCTTAAGATATTCACTGAACATTGATCATCAGAATTCACTTCTGCATGTATCTATAAATTACATGATCGCTTTGCAAATAACTTGTTTTATAATAATCTTGTACATTTATTAGTATTATTAGGGTAGTGCAGCTGAGCCGTTGTTCCGTTGAACCACCATGTTGGTTTGGTTTGTAAAGATATTCGATAGATTAGATCTACCTAGCACTTACACTTATGAACAATTACCGAAAACGTGTCTGGTGTCCGACTGTTGTCACCGACACTCTTGGTTACATTCAATTATGCCATTTCTTTTTTAAATAATTACCGCTGTCAATGTGTAACTATCCATGTTTGTGTTTGTGTGTGTCTGTGTCGGTGATTCATAGCATTAAATATATTATTAAATAGGTACCCCTACAAATTAATCAAAATCAGTTAACGATTTTGTTTATTTGTTTGTGCTTTTAAATTGTGCCCTGTAAATGTATATTGGCTGAAGAAAGTCCGACACTATGCCTGTATTTTCTATATCTTCTCAATTGATTGCTGTAAAATATATGGTGATTTTTTAAACAGTTATGCTATATAACGCGAGAGATTTCTCGCGAATTCCTCAAGAATAACAAAATGCAGTTGTAACAAAAAAGTAAAACAAAAAAAGAGGTCCCACTTACCAAACATGTGGGTTGCCATAATAAAAGAAATAGCGGTTCTTGATGTTTTCTTGAAAAAAAATTCGCTGAATTCAGCATTTTCCTTTTTTAAATGCTTAGTTCGGAATGCTAATAATAAGCCTTTTGGGTATATTGAAATGCAGAGCTTTCAATTGGATAGAAAAAGTGGAAACAAGTGTTACATGCTTGCTGCTAGATCTCTCGCTATTACTTGGGGTGATGATGACCGCTATTGGATCTGGACCACTATGCCTGATTCCAGGTTGGCCATTTTTCCTCCCCAAATTTACTCATTTTTATTGGTTTCTCCACCTTCTACCTTGTTTGTTTTAACATTGTTCTTTCTCCTTTCTCGGTGAATTGTTCATTCCATTTCCTTGGATATTTCGTATCTGAGTTTATACTTTGATTGATCATATTCCCCTCATTAAGGAAACTTTCATCTATGCAAGATGAACGACAATGAGAGGTTGAATATACCCATAGTCTTTGGCTTTTCTTCTATGAAAGATGAAAAACAATGAGAAGTTGAATATACATAAAGAAGTTAAATGAAAATATTTTTCATTTCAGCTTCTCTCACTGTCTCTTCATTTTTCCTTCTAAGTGAATACTTCTTGTTAGACTATTCAATCCCAAAGTTAACCATACACCTGAATCTGAACCTCAATAACAGTGATGTTGGTCCACTGTCTAATAACTTTTTTTTATAGATAACAAACAATACAACTTAGTAACTGAGAAGTTTATCATAATCGATTACACTTGAAATAATTTAGTTATCATTGGTACAATATATGATAACTGATGTTTTTATTAGTTGAGCTGTTTCAAAGTTTTTTTGGTGAAAATATTTTGCTGTTATTACTCTTTTTGATGTTGGAAACTTTGATTCAACTCGCAAATTTTAGTTGATGAGCTTGCATTGTTTGTGTACGTACACATGTATAAATATTCTATTTATGATGTTATGAATCCTTTTCAACTTTTTCTCAATTCTTGTATTCTCATCTCTGCAACTTCTGTGTTGGACAGGTTCCCCGAAGTTGCTAATCTTCGTCTTGTGTGGTGGCTCGAAATTCGTGGGATGATTAACAATCTTGCCTTGTCTCCAAATACTCAGTATGCAGCTTATCTTGTATACAAATTGATTGATGGCTATGGGTTTGAGAACCTTCCTGTGGATTTATCTGTCGGTGTCGAAGGTGGCCATAGTAGCACTAAAATTGTCTGTTTGGATCCAAATGTAGAACGCAGGCAGAACCGCAGACATGGAAGACTTTACGGTCGTGCGAGGTTGCATGACAGAGTGGTAGGATTACCGCGTCCTTGTGTGAGAAGTGATGGGTGGTTGGAGATTGAGATGGGAGAGTTCTTCAATTCAGGCCTTGAAAATGAAGAAGTCCAGATGAGTGTTATCGAGATAAAGGCTGGTGAGACCAAGGGGAATTTCTTTCTTGAGGGAATAGAAGTGAGGCCTAAGGTAGACAATTAA
Protein sequence

>MS.gene49088.t1

MTGFEDLAEGCISSILSRTTPVDAGRLSVVSKTFLSAADSDAVWNHFLPSDIDSIISQSPSLANAPSKKALYLALSDRPIIIDDAKKSFQLDRKSGNKCYMLAARSLAITWGDDDRYWIWTTMPDSRFPEVANLRLVWWLEIRGMINNLALSPNTQYAAYLVYKLIDGYGFENLPVDLSVGVEGGHSSTKIVCLDPNVERRQNRRHGRLYGRARLHDRVVGLPRPCVRSDGWLEIEMGEFFNSGLENEEVQMSVIEIKAGETKGNFFLEGIEVRPKVDN