AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069492

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069492.t1 MTR_1g079370 78.676 272 51 3 1 272 1 265 5.97e-153 427
MS.gene069492.t1 MTR_1g079180 73.529 272 65 3 1 272 1 265 9.70e-143 402
MS.gene069492.t1 MTR_1g079380 77.737 274 54 3 1 274 1 267 8.10e-140 395
MS.gene069492.t1 MTR_1g079460 68.727 275 80 3 1 275 1 269 2.78e-132 375
MS.gene069492.t1 MTR_1g079200 68.613 274 74 5 1 272 1 264 3.09e-126 360
MS.gene069492.t1 MTR_1g079260 63.830 282 88 5 1 275 1 275 1.70e-122 351
MS.gene069492.t1 MTR_1g100597 62.937 286 84 6 1 275 1 275 7.19e-118 339
MS.gene069492.t1 MTR_1g079850 60.069 288 98 4 1 275 1 284 1.18e-112 326
MS.gene069492.t1 MTR_1g079250 59.011 283 106 3 1 275 1 281 4.18e-110 320
MS.gene069492.t1 MTR_1g079230 58.511 282 104 4 1 275 1 276 1.64e-107 313
MS.gene069492.t1 MTR_1g100613 64.257 249 73 5 15 252 1 244 2.71e-105 306
MS.gene069492.t1 MTR_1g026510 53.472 288 119 4 1 275 1 286 1.82e-104 306
MS.gene069492.t1 MTR_3g452600 56.884 276 106 5 1 275 7 270 1.30e-99 292
MS.gene069492.t1 MTR_3g006850 54.426 305 103 5 4 275 17 318 4.14e-98 290
MS.gene069492.t1 MTR_1g079350 56.631 279 110 5 1 272 1 275 4.20e-96 284
MS.gene069492.t1 MTR_3g450990 52.397 292 110 7 1 275 7 286 2.36e-90 270
MS.gene069492.t1 MTR_1g079410 53.430 277 114 6 2 272 6 273 4.96e-90 268
MS.gene069492.t1 MTR_2g079100 54.275 269 106 4 3 269 8 261 1.32e-85 257
MS.gene069492.t1 MTR_1g079340 51.546 291 117 7 1 272 40 325 9.46e-81 246
MS.gene069492.t1 MTR_1g079140 45.151 299 125 7 6 272 15 306 2.69e-77 237
MS.gene069492.t1 MTR_7g096300 54.941 253 97 7 1 248 1 241 6.78e-77 248
MS.gene069492.t1 MTR_1g078470 65.537 177 52 4 96 272 17 184 5.61e-72 219
MS.gene069492.t1 MTR_1g079400 46.233 292 114 6 2 272 10 279 4.91e-70 218
MS.gene069492.t1 MTR_7g112550 41.608 286 147 7 2 273 5 284 7.19e-61 194
MS.gene069492.t1 MTR_1g083580 38.028 284 151 9 4 272 8 281 1.21e-54 178
MS.gene069492.t1 MTR_1g079240 56.831 183 64 5 90 272 12 179 3.79e-54 173
MS.gene069492.t1 MTR_7g112560 36.667 270 157 5 4 272 1 257 1.61e-51 169
MS.gene069492.t1 MTR_1g079350 53.892 167 69 3 1 162 1 164 1.34e-48 160
MS.gene069492.t1 MTR_4g023570 35.316 269 142 6 4 272 1 237 7.73e-46 154
MS.gene069492.t1 MTR_1g076870 60.448 134 36 2 131 248 2 134 7.60e-45 148
MS.gene069492.t1 MTR_1g079130 42.211 199 75 5 104 272 1 189 1.34e-41 142
MS.gene069492.t1 MTR_2g042540 60.748 107 39 2 1 106 1 105 1.31e-34 122
MS.gene069492.t1 MTR_1g079210 54.032 124 44 2 132 243 7 129 2.45e-33 119
MS.gene069492.t1 MTR_1g078460 66.667 90 27 1 1 90 64 150 2.97e-32 116
MS.gene069492.t1 MTR_7g096310 51.908 131 55 3 60 188 38 162 6.37e-31 113
MS.gene069492.t1 MTR_3g058640 56.311 103 42 2 131 233 7 106 6.98e-28 103
MS.gene069492.t1 MTR_0113s0040 36.986 146 90 2 2 147 20 163 1.53e-22 94.4
MS.gene069492.t1 MTR_4g083970 26.042 192 117 4 83 272 122 290 1.77e-18 83.6
MS.gene069492.t1 MTR_1g079220 66.176 68 20 2 168 235 1 65 1.83e-18 78.2
MS.gene069492.t1 MTR_7g103380 24.324 259 166 9 4 247 19 262 4.46e-18 82.4
MS.gene069492.t1 MTR_1g101390 32.450 151 100 2 2 152 26 174 2.34e-17 80.5
MS.gene069492.t1 MTR_0002s0020 30.303 165 106 4 4 163 22 182 3.83e-16 77.0
MS.gene069492.t1 MTR_4g102770 32.877 146 97 1 4 149 19 163 1.01e-15 75.9
MS.gene069492.t1 MTR_1g079390 62.500 56 16 2 212 267 5 55 5.43e-15 68.2
MS.gene069492.t1 MTR_5g010800 30.065 153 97 4 2 150 15 161 4.82e-14 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069492.t1 AT2G02340 40.659 273 150 6 4 271 36 301 6.68e-69 216
MS.gene069492.t1 AT2G02360 42.435 271 137 7 4 271 14 268 6.81e-68 212
MS.gene069492.t1 AT1G80110 43.657 268 138 5 4 271 1 255 7.12e-66 206
MS.gene069492.t1 AT2G02250 39.706 272 149 7 2 271 45 303 1.45e-60 194
MS.gene069492.t1 AT2G02240 40.226 266 145 6 7 271 64 316 1.94e-60 194
MS.gene069492.t1 AT1G09155 39.298 285 147 9 7 272 3 280 5.60e-52 171
MS.gene069492.t1 AT2G02230 32.484 314 161 8 4 275 32 336 8.65e-52 172
MS.gene069492.t1 AT1G56240 39.085 284 147 7 7 271 4 280 1.48e-51 170
MS.gene069492.t1 AT2G02350 37.591 274 154 9 2 271 22 282 1.67e-49 165
MS.gene069492.t1 AT1G56250 38.947 285 148 7 7 272 4 281 3.18e-49 164
MS.gene069492.t1 AT2G02300 36.861 274 142 8 4 272 34 281 3.80e-46 156
MS.gene069492.t1 AT2G02320 36.765 272 151 9 6 271 42 298 1.03e-45 156
MS.gene069492.t1 AT2G02310 33.456 272 166 7 4 272 45 304 5.56e-43 149
MS.gene069492.t1 AT5G24560 45.070 142 72 2 6 147 5 140 2.67e-37 131
MS.gene069492.t1 AT2G02350 35.465 172 100 6 104 271 1 165 6.65e-24 95.5
MS.gene069492.t1 AT4G19850 31.148 183 96 8 97 272 17 176 4.50e-18 80.1
MS.gene069492.t1 AT4G19850 31.148 183 96 8 97 272 59 218 5.16e-18 80.9
MS.gene069492.t1 AT3G53000 31.944 144 95 2 6 149 23 163 6.10e-18 82.0
MS.gene069492.t1 AT3G61060 27.545 167 117 3 6 172 27 189 3.61e-16 77.0
MS.gene069492.t1 AT5G52120 32.895 152 96 2 4 150 21 171 1.83e-15 75.1
MS.gene069492.t1 AT1G33920 26.286 175 102 5 103 272 12 164 8.89e-15 70.9
MS.gene069492.t1 AT3G61060 25.749 167 121 2 6 172 27 190 9.20e-15 73.2
MS.gene069492.t1 AT1G12710 31.507 146 95 3 6 150 30 171 6.02e-14 70.9
MS.gene069492.t1 AT1G63090 27.711 166 113 4 4 168 26 185 2.81e-13 68.9
MS.gene069492.t1 AT4G19840 25.794 252 140 9 47 272 15 245 3.25e-13 68.2
MS.gene069492.t1 AT4G19850 31.944 144 72 6 97 234 59 182 4.65e-12 64.3

Find 74 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGCTGCATATTGAGTATT+TGG 0.137691 6.4:+53452818 None:intergenic
CTACCTTCCACTTCCACATT+TGG 0.297421 6.4:+53452686 None:intergenic
CGACCATTGTTGATGATGAT+AGG 0.306822 6.4:+53453466 None:intergenic
TGTCCGATTCCGACGATGTC+TGG 0.321307 6.4:-53453600 MS.gene069492:CDS
TACAAAATGATTAATGCTTC+GGG 0.325580 6.4:-53452788 MS.gene069492:CDS
TACTAATAAAATTGTCTGTT+TGG 0.332954 6.4:-53452711 MS.gene069492:CDS
AAGCTGCATATTGAGTATTT+GGG 0.334659 6.4:+53452819 None:intergenic
CTAGATCTCTTGCAATTACT+TGG 0.341041 6.4:-53453298 MS.gene069492:CDS
GTACAAAATGATTAATGCTT+CGG 0.347055 6.4:-53452789 MS.gene069492:CDS
AGATGATAGAGTCGAAGAAT+TGG 0.353931 6.4:+53453554 None:intergenic
TCAACAAGCTTAGCGACTTC+AGG 0.398511 6.4:+53452890 None:intergenic
ATGAATGTAGAGCTTTCAAT+TGG 0.402958 6.4:-53453357 MS.gene069492:intron
GTGGTGGCTGTCCTGCTAAT+TGG 0.412051 6.4:-53452532 MS.gene069492:CDS
GGAAACCACGGAGAGTCTTC+CGG 0.428101 6.4:+53453639 None:intergenic
ATTCCAGACATCGTCGGAAT+CGG 0.431508 6.4:+53453597 None:intergenic
GGGGTGATACCGAGCACTAT+TGG 0.432942 6.4:-53453277 MS.gene069492:CDS
AGAAAGAATGACAGTGATTG+AGG 0.442308 6.4:-53453716 None:intergenic
TAGATCTCTTGCAATTACTT+GGG 0.448620 6.4:-53453297 MS.gene069492:CDS
GATGATAGAGTCGAAGAATT+GGG 0.449823 6.4:+53453555 None:intergenic
TTCTTGAAGGAATAGAAGTT+AGG 0.449929 6.4:-53452496 MS.gene069492:CDS
ATACCGAGCACTATTGGAAC+TGG 0.453489 6.4:-53453271 MS.gene069492:CDS
GGGTGGTTGGAGATTGAGAT+GGG 0.458032 6.4:-53452614 MS.gene069492:CDS
AAGAGAGGGCTCGTTCTTGA+AGG 0.466484 6.4:-53452509 MS.gene069492:CDS
GTTGGTTAATTGTTATACTT+AGG 0.467370 6.4:+53452473 None:intergenic
CGTCGGAATCGGACACAGAA+TGG 0.468542 6.4:+53453608 None:intergenic
TTGTCGGTAACAAATTCATC+TGG 0.477960 6.4:+53452555 None:intergenic
GACCATTGTTGATGATGATA+GGG 0.489358 6.4:+53453467 None:intergenic
TGGAGTTGTACGAGACACTA+TGG 0.490376 6.4:+53453669 None:intergenic
AGGCAGCACAATGGAGAAGT+AGG 0.490899 6.4:-53452665 MS.gene069492:CDS
CATATTGAGTATTTGGGGAC+AGG 0.495017 6.4:+53452825 None:intergenic
GTGTGTTTGCTTGAAATTCG+TGG 0.498970 6.4:-53452866 MS.gene069492:CDS
GGATTTCTTTGCCAGAGTCC+AGG 0.500655 6.4:-53453250 MS.gene069492:intron
AGCCCTCTCTTCCAATTAGC+AGG 0.503797 6.4:+53452521 None:intergenic
GATTTATCAGTTTGTGTCGA+AGG 0.510672 6.4:-53452746 MS.gene069492:CDS
AGTCCTAGTGTGAGAAGCGA+TGG 0.513391 6.4:-53452635 MS.gene069492:CDS
TGGGTGGTTGGAGATTGAGA+TGG 0.517146 6.4:-53452615 MS.gene069492:CDS
GGACACAGAATGGAAAGTCT+TGG 0.528487 6.4:+53453618 None:intergenic
GAGAGTCTTCCGGCGTCGAG+TGG 0.537858 6.4:+53453649 None:intergenic
GCAGGACAGCCACCACTTGT+CGG 0.538246 6.4:+53452539 None:intergenic
CACCCATCGCTTCTCACACT+AGG 0.543006 6.4:+53452632 None:intergenic
CGAAGAATTGGGAATCGGAC+GGG 0.555681 6.4:+53453566 None:intergenic
AGAGTCGAAGAATTGGGAAT+CGG 0.556197 6.4:+53453561 None:intergenic
ATGAATTTGTTACCGACAAG+TGG 0.560774 6.4:-53452551 MS.gene069492:CDS
TTTGGATCCAAATGTGGAAG+TGG 0.569537 6.4:-53452693 MS.gene069492:CDS
AGATCTCTTGCAATTACTTG+GGG 0.575236 6.4:-53453296 MS.gene069492:CDS
TGTGAGAAGCGATGGGTGGT+TGG 0.576323 6.4:-53452627 MS.gene069492:CDS
CAAATGTGGAAGTGGAAGGT+AGG 0.580778 6.4:-53452685 MS.gene069492:CDS
GTCCTGCTAATTGGAAGAGA+GGG 0.588891 6.4:-53452523 MS.gene069492:CDS
CATCATCAACAATGGTCGAA+AGG 0.589028 6.4:-53453461 MS.gene069492:intron
GTAGGAATCTTAGCAAGGGA+AGG 0.592381 6.4:+53453523 None:intergenic
GTGGTTGGAGATTGAGATGG+GGG 0.596500 6.4:-53452612 MS.gene069492:CDS
GATCCAAATGTGGAAGTGGA+AGG 0.597012 6.4:-53452689 MS.gene069492:CDS
AGCTGCATATTGAGTATTTG+GGG 0.601395 6.4:+53452820 None:intergenic
AATCCAGTTCCAATAGTGCT+CGG 0.601607 6.4:+53453268 None:intergenic
TCGAAGAATTGGGAATCGGA+CGG 0.601854 6.4:+53453565 None:intergenic
TTATCAGTTTGTGTCGAAGG+TGG 0.604532 6.4:-53452743 MS.gene069492:CDS
AGTATTTGGGGACAGGACAA+TGG 0.604576 6.4:+53452832 None:intergenic
CGCCCTATCATCATCAACAA+TGG 0.605291 6.4:-53453469 MS.gene069492:CDS
TTTGGTAGGAATCTTAGCAA+GGG 0.614613 6.4:+53453519 None:intergenic
GTGATTGAGGAATTGCAAGA+AGG 0.618748 6.4:-53453703 MS.gene069492:CDS
GGTGGTTGGAGATTGAGATG+GGG 0.619250 6.4:-53452613 MS.gene069492:CDS
TGTCCTGCTAATTGGAAGAG+AGG 0.619513 6.4:-53452524 MS.gene069492:CDS
TGTCTGTTTGGATCCAAATG+TGG 0.625217 6.4:-53452699 MS.gene069492:CDS
GAGCTAACTAACCTGGACTC+TGG 0.625509 6.4:+53453239 None:intergenic
GTCCTAGTGTGAGAAGCGAT+GGG 0.635171 6.4:-53452634 MS.gene069492:CDS
AATTTGTTACCGACAAGTGG+TGG 0.669199 6.4:-53452548 MS.gene069492:CDS
ATGGAAAGTCTTGGAAACCA+CGG 0.671010 6.4:+53453627 None:intergenic
CGTACAACTCCACTCGACGC+CGG 0.671498 6.4:-53453658 MS.gene069492:CDS
GTGGAAGGTAGGCAGCACAA+TGG 0.674376 6.4:-53452674 MS.gene069492:CDS
CGACGCCGGAAGACTCTCCG+TGG 0.694576 6.4:-53453644 MS.gene069492:CDS
AAAGTGATTCCAGACATCGT+CGG 0.706745 6.4:+53453591 None:intergenic
CTAGTGTGAGAAGCGATGGG+TGG 0.726871 6.4:-53452631 MS.gene069492:CDS
AAACTGATAAATCTGCACGA+TGG 0.741386 6.4:+53452756 None:intergenic
AGTTGTACGAGACACTATGG+CGG 0.795927 6.4:+53453672 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTAAAATTAAATATTCA+AGG + chr6.4:53453013-53453032 None:intergenic 10.0%
!!! TTGAATTTTACTTTTTATCA+TGG - chr6.4:53453044-53453063 MS.gene069492:intron 15.0%
!! TACTAATAAAATTGTCTGTT+TGG - chr6.4:53453476-53453495 MS.gene069492:CDS 20.0%
! AAATGACAACATTATGCAAA+AGG + chr6.4:53453099-53453118 None:intergenic 25.0%
! AATTAACATTTCAGAGAGAA+AGG + chr6.4:53452984-53453003 None:intergenic 25.0%
! GTACAAAATGATTAATGCTT+CGG - chr6.4:53453398-53453417 MS.gene069492:intron 25.0%
! TACAAAATGATTAATGCTTC+GGG - chr6.4:53453399-53453418 MS.gene069492:intron 25.0%
! TTGGGTTTATAAAATGCTAA+TGG - chr6.4:53452790-53452809 MS.gene069492:CDS 25.0%
!! CAATTTTATTAGTACTACTC+TGG + chr6.4:53453470-53453489 None:intergenic 25.0%
!! CTAATTAACTGCCAATTTTT+CGG - chr6.4:53452763-53452782 MS.gene069492:CDS 25.0%
!!! CACAAAATAAGATTTTGAGT+TGG + chr6.4:53453124-53453143 None:intergenic 25.0%
AAATTAAGAGCTAACTAACC+TGG + chr6.4:53452958-53452977 None:intergenic 30.0%
AACCCAAATATCCGAAAAAT+TGG + chr6.4:53452777-53452796 None:intergenic 30.0%
AAGCTGCATATTGAGTATTT+GGG + chr6.4:53453371-53453390 None:intergenic 30.0%
AATAGCCAAGGTAATTCAAT+GGG + chr6.4:53453073-53453092 None:intergenic 30.0%
ATGAATGTAGAGCTTTCAAT+TGG - chr6.4:53452830-53452849 MS.gene069492:CDS 30.0%
CGAAAAATTGGCAGTTAATT+AGG + chr6.4:53452765-53452784 None:intergenic 30.0%
TAAGCTGCATATTGAGTATT+TGG + chr6.4:53453372-53453391 None:intergenic 30.0%
TAATAGCCAAGGTAATTCAA+TGG + chr6.4:53453074-53453093 None:intergenic 30.0%
TAGATCTCTTGCAATTACTT+GGG - chr6.4:53452890-53452909 MS.gene069492:CDS 30.0%
TTCTTGAAGGAATAGAAGTT+AGG - chr6.4:53453691-53453710 MS.gene069492:CDS 30.0%
! CATCTTTTGTGTACTACTTT+TGG - chr6.4:53453266-53453285 MS.gene069492:CDS 30.0%
!! TGCCAATTTTTCGGATATTT+GGG - chr6.4:53452772-53452791 MS.gene069492:CDS 30.0%
!!! ACTTTTTCATTTTCTATGCC+TGG + chr6.4:53453612-53453631 None:intergenic 30.0%
!!! AGATAGAGAGATTTTTTGGT+AGG + chr6.4:53452685-53452704 None:intergenic 30.0%
!!! AGCAAGATAGAGAGATTTTT+TGG + chr6.4:53452689-53452708 None:intergenic 30.0%
AAACTGATAAATCTGCACGA+TGG + chr6.4:53453434-53453453 None:intergenic 35.0%
AGATCTCTTGCAATTACTTG+GGG - chr6.4:53452891-53452910 MS.gene069492:CDS 35.0%
AGATGATAGAGTCGAAGAAT+TGG + chr6.4:53452636-53452655 None:intergenic 35.0%
AGCTGCATATTGAGTATTTG+GGG + chr6.4:53453370-53453389 None:intergenic 35.0%
CTAGATCTCTTGCAATTACT+TGG - chr6.4:53452889-53452908 MS.gene069492:CDS 35.0%
GATGATAGAGTCGAAGAATT+GGG + chr6.4:53452635-53452654 None:intergenic 35.0%
GATTTATCAGTTTGTGTCGA+AGG - chr6.4:53453441-53453460 MS.gene069492:intron 35.0%
TTGTCGGTAACAAATTCATC+TGG + chr6.4:53453635-53453654 None:intergenic 35.0%
TTTGGTAGGAATCTTAGCAA+GGG + chr6.4:53452671-53452690 None:intergenic 35.0%
! TTTTGGTAGGAATCTTAGCA+AGG + chr6.4:53452672-53452691 None:intergenic 35.0%
!! ATGAATTTGTTACCGACAAG+TGG - chr6.4:53453636-53453655 MS.gene069492:CDS 35.0%
!! CTGCCAATTTTTCGGATATT+TGG - chr6.4:53452771-53452790 MS.gene069492:CDS 35.0%
!! GACCATTGTTGATGATGATA+GGG + chr6.4:53452723-53452742 None:intergenic 35.0%
!! TTTGTGTACTACTTTTGGAC+AGG - chr6.4:53453271-53453290 MS.gene069492:CDS 35.0%
AAAGTGATTCCAGACATCGT+CGG + chr6.4:53452599-53452618 None:intergenic 40.0%
AATCCAGTTCCAATAGTGCT+CGG + chr6.4:53452922-53452941 None:intergenic 40.0%
AGAGTCGAAGAATTGGGAAT+CGG + chr6.4:53452629-53452648 None:intergenic 40.0%
ATGGAAAGTCTTGGAAACCA+CGG + chr6.4:53452563-53452582 None:intergenic 40.0%
CATATTGAGTATTTGGGGAC+AGG + chr6.4:53453365-53453384 None:intergenic 40.0%
CATCATCAACAATGGTCGAA+AGG - chr6.4:53452726-53452745 MS.gene069492:CDS 40.0%
GGAATCCCATTGAATTACCT+TGG - chr6.4:53453065-53453084 MS.gene069492:intron 40.0%
GTGATTGAGGAATTGCAAGA+AGG - chr6.4:53452484-53452503 MS.gene069492:CDS 40.0%
GTGTGTTTGCTTGAAATTCG+TGG - chr6.4:53453321-53453340 MS.gene069492:CDS 40.0%
TGTCTGTTTGGATCCAAATG+TGG - chr6.4:53453488-53453507 MS.gene069492:CDS 40.0%
TTATCAGTTTGTGTCGAAGG+TGG - chr6.4:53453444-53453463 MS.gene069492:intron 40.0%
TTTGGATCCAAATGTGGAAG+TGG - chr6.4:53453494-53453513 MS.gene069492:CDS 40.0%
! TGCAAAAGGTGTAATAGCCA+AGG + chr6.4:53453085-53453104 None:intergenic 40.0%
!! AATTTGTTACCGACAAGTGG+TGG - chr6.4:53453639-53453658 MS.gene069492:CDS 40.0%
!! CGACCATTGTTGATGATGAT+AGG + chr6.4:53452724-53452743 None:intergenic 40.0%
AGTATTTGGGGACAGGACAA+TGG + chr6.4:53453358-53453377 None:intergenic 45.0%
AGTTGTACGAGACACTATGG+CGG + chr6.4:53452518-53452537 None:intergenic 45.0%
ATACCGAGCACTATTGGAAC+TGG - chr6.4:53452916-53452935 MS.gene069492:intron 45.0%
ATTCCAGACATCGTCGGAAT+CGG + chr6.4:53452593-53452612 None:intergenic 45.0%
CAAATGTGGAAGTGGAAGGT+AGG - chr6.4:53453502-53453521 MS.gene069492:CDS 45.0%
CGCCCTATCATCATCAACAA+TGG - chr6.4:53452718-53452737 MS.gene069492:CDS 45.0%
CTACCTTCCACTTCCACATT+TGG + chr6.4:53453504-53453523 None:intergenic 45.0%
GATCCAAATGTGGAAGTGGA+AGG - chr6.4:53453498-53453517 MS.gene069492:CDS 45.0%
GGACACAGAATGGAAAGTCT+TGG + chr6.4:53452572-53452591 None:intergenic 45.0%
GTAGGAATCTTAGCAAGGGA+AGG + chr6.4:53452667-53452686 None:intergenic 45.0%
GTCCTGCTAATTGGAAGAGA+GGG - chr6.4:53453664-53453683 MS.gene069492:CDS 45.0%
TCAACAAGCTTAGCGACTTC+AGG + chr6.4:53453300-53453319 None:intergenic 45.0%
TCGAAGAATTGGGAATCGGA+CGG + chr6.4:53452625-53452644 None:intergenic 45.0%
TGGAGTTGTACGAGACACTA+TGG + chr6.4:53452521-53452540 None:intergenic 45.0%
TGTCCTGCTAATTGGAAGAG+AGG - chr6.4:53453663-53453682 MS.gene069492:CDS 45.0%
AAGAGAGGGCTCGTTCTTGA+AGG - chr6.4:53453678-53453697 MS.gene069492:CDS 50.0%
AGCCCTCTCTTCCAATTAGC+AGG + chr6.4:53453669-53453688 None:intergenic 50.0%
AGGCAGCACAATGGAGAAGT+AGG - chr6.4:53453522-53453541 MS.gene069492:CDS 50.0%
AGTCCTAGTGTGAGAAGCGA+TGG - chr6.4:53453552-53453571 MS.gene069492:CDS 50.0%
ATGGGGGAGTTCTTCAATCC+AGG - chr6.4:53453591-53453610 MS.gene069492:CDS 50.0%
CAGACACACACACACACACT+TGG - chr6.4:53453186-53453205 MS.gene069492:intron 50.0%
CGAAGAATTGGGAATCGGAC+GGG + chr6.4:53452624-53452643 None:intergenic 50.0%
GAGCTAACTAACCTGGACTC+TGG + chr6.4:53452951-53452970 None:intergenic 50.0%
GGATTTCTTTGCCAGAGTCC+AGG - chr6.4:53452937-53452956 MS.gene069492:intron 50.0%
GGGTGGTTGGAGATTGAGAT+GGG - chr6.4:53453573-53453592 MS.gene069492:CDS 50.0%
GGTGGTTGGAGATTGAGATG+GGG - chr6.4:53453574-53453593 MS.gene069492:CDS 50.0%
GTCCTAGTGTGAGAAGCGAT+GGG - chr6.4:53453553-53453572 MS.gene069492:CDS 50.0%
GTGGTTGGAGATTGAGATGG+GGG - chr6.4:53453575-53453594 MS.gene069492:CDS 50.0%
GTGTGTGTGTCTGTGTGTCA+GGG + chr6.4:53453179-53453198 None:intergenic 50.0%
TGGGTGGTTGGAGATTGAGA+TGG - chr6.4:53453572-53453591 MS.gene069492:CDS 50.0%
TGTGTGTGTGTCTGTGTGTC+AGG + chr6.4:53453180-53453199 None:intergenic 50.0%
CACCCATCGCTTCTCACACT+AGG + chr6.4:53453558-53453577 None:intergenic 55.0%
CGTCGGAATCGGACACAGAA+TGG + chr6.4:53452582-53452601 None:intergenic 55.0%
CTAGTGTGAGAAGCGATGGG+TGG - chr6.4:53453556-53453575 MS.gene069492:CDS 55.0%
GGAAACCACGGAGAGTCTTC+CGG + chr6.4:53452551-53452570 None:intergenic 55.0%
GGGGTGATACCGAGCACTAT+TGG - chr6.4:53452910-53452929 MS.gene069492:intron 55.0%
GTGGAAGGTAGGCAGCACAA+TGG - chr6.4:53453513-53453532 MS.gene069492:CDS 55.0%
GTGGTGGCTGTCCTGCTAAT+TGG - chr6.4:53453655-53453674 MS.gene069492:CDS 55.0%
TGTCCGATTCCGACGATGTC+TGG - chr6.4:53452587-53452606 MS.gene069492:CDS 55.0%
TGTGAGAAGCGATGGGTGGT+TGG - chr6.4:53453560-53453579 MS.gene069492:CDS 55.0%
CGTACAACTCCACTCGACGC+CGG - chr6.4:53452529-53452548 MS.gene069492:CDS 60.0%
GCAGGACAGCCACCACTTGT+CGG + chr6.4:53453651-53453670 None:intergenic 60.0%
! GAGAGTCTTCCGGCGTCGAG+TGG + chr6.4:53452541-53452560 None:intergenic 65.0%
CGACGCCGGAAGACTCTCCG+TGG - chr6.4:53452543-53452562 MS.gene069492:CDS 70.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 53452478 53453731 53452478 ID=MS.gene069492
chr6.4 mRNA 53452478 53453731 53452478 ID=MS.gene069492.t1;Parent=MS.gene069492
chr6.4 exon 53453462 53453731 53453462 ID=MS.gene069492.t1.exon1;Parent=MS.gene069492.t1
chr6.4 CDS 53453462 53453731 53453462 ID=cds.MS.gene069492.t1;Parent=MS.gene069492.t1
chr6.4 exon 53453251 53453369 53453251 ID=MS.gene069492.t1.exon2;Parent=MS.gene069492.t1
chr6.4 CDS 53453251 53453369 53453251 ID=cds.MS.gene069492.t1;Parent=MS.gene069492.t1
chr6.4 exon 53452478 53452916 53452478 ID=MS.gene069492.t1.exon3;Parent=MS.gene069492.t1
chr6.4 CDS 53452478 53452916 53452478 ID=cds.MS.gene069492.t1;Parent=MS.gene069492.t1
Gene Sequence

>MS.gene069492

ATGACAGTGATTGAGGAATTGCAAGAAGGCTGCATTGCCGCCATAGTGTCTCGTACAACTCCACTCGACGCCGGAAGACTCTCCGTGGTTTCCAAGACTTTCCATTCTGTGTCCGATTCCGACGATGTCTGGAATCACTTTCTCCCGTCCGATTCCCAATTCTTCGACTCTATCATCTCACACACTCCTTCCCTTGCTAAGATTCCTACCAAAAAATCTCTCTATCTTGCTCTATCTGATCGCCCTATCATCATCAACAATGGTCGAAAGGTATTCTTCTTATTCCTAATTAACTGCCAATTTTTCGGATATTTGGGTTTATAAAATGCTAATGGAAATTAAATGTGTTGAAATGAATGTAGAGCTTTCAATTGGATAGAAAGAGTAGAAAAATTTGTTACATGCTTTCTGCTAGATCTCTTGCAATTACTTGGGGTGATACCGAGCACTATTGGAACTGGATTTCTTTGCCAGAGTCCAGGTTAGTTAGCTCTTAATTTTCTCCTTTCTCTCTGAAATGTTAATTACATTTCCTTGAATATTTAATTTTACTATAAGACTTGTTATTGAATTTTACTTTTTATCATGGAATCCCATTGAATTACCTTGGCTATTACACCTTTTGCATAATGTTGTCATTTGTCCAACTCAAAATCTTATTTTGTGACAATCAAGCAGTTGTCTATATTAGTTAACGACCCTGACACACAGACACACACACACACACTTGGTTTGTTATGTAACAAAACGCTTTCAACTTTTCTGTCAGTTATTACGTTCAATCTTTGCATCTTTTGTGTACTACTTTTGGACAGGTTTCCTGAAGTCGCTAAGCTTGTTGATGTGTGTTTGCTTGAAATTCGTGGTATGATAAACACCATTGTCCTGTCCCCAAATACTCAATATGCAGCTTATGTTGTGTACAAAATGATTAATGCTTCGGGATTTCAGAACCATCGTGCAGATTTATCAGTTTGTGTCGAAGGTGGCCAGAGTAGTACTAATAAAATTGTCTGTTTGGATCCAAATGTGGAAGTGGAAGGTAGGCAGCACAATGGAGAAGTAGGATTGAAAAGTCCTAGTGTGAGAAGCGATGGGTGGTTGGAGATTGAGATGGGGGAGTTCTTCAATCCAGGCATAGAAAATGAAAAAGTCCAGATGAATTTGTTACCGACAAGTGGTGGCTGTCCTGCTAATTGGAAGAGAGGGCTCGTTCTTGAAGGAATAGAAGTTAGGCCTAAGTATAACAATTAA
Protein sequence

>MS.gene069492.t1

MTVIEELQEGCIAAIVSRTTPLDAGRLSVVSKTFHSVSDSDDVWNHFLPSDSQFFDSIISHTPSLAKIPTKKSLYLALSDRPIIINNGRKSFQLDRKSRKICYMLSARSLAITWGDTEHYWNWISLPESRFPEVAKLVDVCLLEIRGMINTIVLSPNTQYAAYVVYKMINASGFQNHRADLSVCVEGGQSSTNKIVCLDPNVEVEGRQHNGEVGLKSPSVRSDGWLEIEMGEFFNPGIENEKVQMNLLPTSGGCPANWKRGLVLEGIEVRPKYNN