AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27114

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27114.t1 MTR_1g026510 96.678 301 10 0 1 301 1 301 0.0 603
MS.gene27114.t1 MTR_1g079250 73.422 301 75 1 1 301 1 296 1.54e-160 449
MS.gene27114.t1 MTR_1g079260 63.636 286 93 4 1 286 1 275 7.78e-127 363
MS.gene27114.t1 MTR_1g079180 63.251 283 86 6 1 283 1 265 1.25e-116 337
MS.gene27114.t1 MTR_1g079460 61.538 286 93 5 1 286 1 269 6.49e-112 325
MS.gene27114.t1 MTR_1g079370 59.011 283 98 6 1 283 1 265 1.18e-110 321
MS.gene27114.t1 MTR_1g079200 61.131 283 91 5 1 283 1 264 4.13e-110 320
MS.gene27114.t1 MTR_1g100597 61.246 289 95 8 1 286 1 275 2.72e-108 316
MS.gene27114.t1 MTR_1g079380 56.040 298 112 7 1 297 1 280 3.15e-101 298
MS.gene27114.t1 MTR_3g006850 54.397 307 115 5 2 286 15 318 6.15e-98 291
MS.gene27114.t1 MTR_1g079850 54.949 293 116 6 1 286 1 284 7.20e-97 287
MS.gene27114.t1 MTR_1g079230 53.082 292 115 6 1 286 1 276 7.35e-94 279
MS.gene27114.t1 MTR_1g079350 52.962 287 119 7 1 283 1 275 9.00e-90 269
MS.gene27114.t1 MTR_3g452600 53.873 284 107 7 4 286 10 270 1.60e-89 268
MS.gene27114.t1 MTR_1g100613 56.504 246 91 5 20 260 5 239 5.00e-84 253
MS.gene27114.t1 MTR_3g450990 50.333 300 109 10 4 286 10 286 2.00e-80 245
MS.gene27114.t1 MTR_2g079100 48.443 289 121 7 3 289 8 270 6.51e-76 233
MS.gene27114.t1 MTR_1g079410 45.804 286 133 8 3 284 7 274 6.11e-73 226
MS.gene27114.t1 MTR_7g096300 50.725 276 110 7 1 273 1 253 3.13e-72 236
MS.gene27114.t1 MTR_1g079340 46.230 305 123 9 1 283 40 325 3.18e-70 221
MS.gene27114.t1 MTR_1g079140 41.333 300 150 5 4 283 13 306 4.64e-66 209
MS.gene27114.t1 MTR_1g079400 40.476 294 135 9 4 283 12 279 1.33e-57 187
MS.gene27114.t1 MTR_1g078470 56.383 188 62 6 96 283 17 184 1.47e-56 181
MS.gene27114.t1 MTR_7g112550 38.408 289 153 11 7 284 10 284 3.79e-53 175
MS.gene27114.t1 MTR_1g083580 37.884 293 150 11 4 283 8 281 1.26e-52 174
MS.gene27114.t1 MTR_1g079240 48.705 193 73 5 91 283 13 179 8.04e-46 153
MS.gene27114.t1 MTR_1g079350 59.420 138 49 2 1 136 1 133 1.38e-45 154
MS.gene27114.t1 MTR_7g112560 33.451 284 158 8 4 283 1 257 2.96e-41 144
MS.gene27114.t1 MTR_1g076870 54.745 137 47 4 136 261 2 134 1.09e-39 136
MS.gene27114.t1 MTR_2g042540 59.813 107 40 2 1 106 1 105 2.67e-35 124
MS.gene27114.t1 MTR_4g023570 31.560 282 146 9 4 283 1 237 9.53e-35 126
MS.gene27114.t1 MTR_1g079130 38.974 195 98 4 104 283 1 189 7.32e-34 122
MS.gene27114.t1 MTR_1g078460 63.095 84 28 1 1 84 64 144 4.32e-30 111
MS.gene27114.t1 MTR_7g096310 45.638 149 62 4 58 196 24 163 1.15e-28 108
MS.gene27114.t1 MTR_1g079210 50.794 126 53 4 137 256 7 129 3.64e-27 103
MS.gene27114.t1 MTR_3g058640 48.649 111 46 3 136 246 7 106 1.49e-22 90.5
MS.gene27114.t1 MTR_1g079220 54.545 77 24 3 173 249 1 66 2.65e-17 75.5
MS.gene27114.t1 MTR_1g079390 67.308 52 14 2 227 278 7 55 5.30e-14 65.9
MS.gene27114.t1 MTR_4g083970 27.461 193 102 8 94 283 133 290 5.56e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27114.t1 AT1G80110 39.350 277 142 6 7 282 4 255 7.90e-55 179
MS.gene27114.t1 AT2G02250 37.634 279 152 7 4 282 47 303 3.22e-54 179
MS.gene27114.t1 AT2G02360 36.299 281 151 6 4 282 14 268 1.74e-53 176
MS.gene27114.t1 AT2G02240 34.629 283 162 7 4 286 61 320 1.57e-51 172
MS.gene27114.t1 AT2G02230 32.492 317 168 8 4 286 32 336 7.67e-49 166
MS.gene27114.t1 AT1G09155 36.332 289 161 9 7 283 3 280 1.75e-45 155
MS.gene27114.t1 AT1G56240 34.722 288 165 8 7 282 4 280 1.26e-44 153
MS.gene27114.t1 AT2G02340 33.793 290 163 8 1 282 33 301 1.97e-44 153
MS.gene27114.t1 AT2G02350 31.849 292 175 6 2 291 22 291 2.07e-43 150
MS.gene27114.t1 AT1G56250 35.294 289 164 8 7 283 4 281 4.67e-43 149
MS.gene27114.t1 AT2G02300 30.690 290 155 10 2 284 32 282 1.20e-35 130
MS.gene27114.t1 AT2G02310 30.742 283 172 7 4 284 45 305 2.85e-35 129
MS.gene27114.t1 AT2G02320 31.119 286 159 10 6 282 42 298 1.57e-34 127
MS.gene27114.t1 AT5G24560 41.558 154 79 3 4 157 3 145 9.66e-29 109
MS.gene27114.t1 AT2G02350 30.000 190 115 5 104 291 1 174 5.50e-20 85.9
MS.gene27114.t1 AT3G53000 24.252 301 181 12 6 290 23 292 6.38e-16 77.0
MS.gene27114.t1 AT1G33920 27.419 186 97 7 103 283 12 164 1.25e-11 62.4
MS.gene27114.t1 AT4G19850 29.442 197 92 9 97 283 59 218 2.51e-11 62.8
MS.gene27114.t1 AT4G19850 28.426 197 94 8 97 283 17 176 2.59e-11 62.0

Find 90 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTACCAAAATGACAATTT+TGG 0.213350 1.1:-78615 None:intergenic
ATGGTTGATGGCTTTGGTTT+TGG 0.220885 1.1:+78536 MS.gene27114:CDS
TAGAACCAAAATTGTCATTT+TGG 0.238661 1.1:+78610 MS.gene27114:CDS
CCGCCGACTCCGATGAAATC+TGG 0.286656 1.1:+77147 MS.gene27114:CDS
GCTGCTAGATCTCTCTCTTT+AGG 0.290498 1.1:+77435 MS.gene27114:CDS
TAGGCTGGGCCGGCGTTGCT+TGG 0.291939 1.1:+77454 MS.gene27114:CDS
ATGGGAGAGTTCTTCAATTC+AGG 0.292340 1.1:+78746 MS.gene27114:CDS
GAAGTCCAAATGAGTATTAC+TGG 0.304926 1.1:+78782 MS.gene27114:CDS
TTAAGAATCTCAACAACTTC+AGG 0.315871 1.1:-78428 None:intergenic
TAAGTTGCATATGTAGTATT+CGG 0.322872 1.1:-78500 None:intergenic
TATTGATTATTATCTTCCTT+TGG 0.333817 1.1:-78863 None:intergenic
TTATCCCAGTAATACTCATT+TGG 0.337010 1.1:-78787 None:intergenic
GAGAGCCTGCCGACGTCAAC+AGG 0.341542 1.1:-77098 None:intergenic
GGCGAGATAGAATGCTCTTT+TGG 0.351997 1.1:-77246 None:intergenic
CACCCATCACTTCTCATTCT+AGG 0.353018 1.1:-78710 None:intergenic
AATGGGAGCGTTTGAAGAAT+TGG 0.354251 1.1:+77037 None:intergenic
GGACTTCTATGTCGGATTCC+AGG 0.358513 1.1:+77502 MS.gene27114:CDS
AATCAATATAAACTAAACTA+TGG 0.359047 1.1:+78878 MS.gene27114:CDS
AATGAGTATTACTGGGATAA+AGG 0.362381 1.1:+78790 MS.gene27114:CDS
ATCCGCCTACATTTCACGTT+TGG 0.365825 1.1:-78638 None:intergenic
AGGCGGATCAACAAAATATT+AGG 0.370152 1.1:+78653 MS.gene27114:CDS
TTCAAGATGGTTGATGGCTT+TGG 0.374603 1.1:+78530 MS.gene27114:CDS
CTAGATCTCTCTCTTTAGGC+TGG 0.381827 1.1:+77439 MS.gene27114:CDS
AAGTTGCATATGTAGTATTC+GGG 0.388989 1.1:-78499 None:intergenic
CTTGTGTTCAAGATGGTTGA+TGG 0.390765 1.1:+78524 MS.gene27114:CDS
TTGTTGAAGGAATAGAAGTT+AGG 0.402827 1.1:+78840 MS.gene27114:CDS
AGGCTGGGCCGGCGTTGCTT+GGG 0.405783 1.1:+77455 MS.gene27114:CDS
CAACAAAATATTAGGTAGTC+AGG 0.424037 1.1:+78661 MS.gene27114:CDS
AAGAGAGGTTTCTTTGTTGA+AGG 0.432696 1.1:+78827 MS.gene27114:CDS
GCGAGTGAGGATAGGATGAT+TGG 0.443568 1.1:-77273 None:intergenic
CGCCGACTCCGATGAAATCT+GGG 0.449269 1.1:+77148 MS.gene27114:CDS
GATGATATCGGCTATGAGTT+GGG 0.450854 1.1:-77192 None:intergenic
TATCCTGTGGAATTATCGTT+TGG 0.462656 1.1:+78569 MS.gene27114:CDS
CGACTCCTGTTGACGTCGGC+AGG 0.489769 1.1:+77093 MS.gene27114:CDS
TATGGAAGGGTCTTGTGTCT+TGG 0.493873 1.1:+78896 MS.gene27114:CDS
AGGAAACTGTGAGATGATAT+CGG 0.495713 1.1:-77204 None:intergenic
CGATCATCGCGAGTGAGGAT+AGG 0.501028 1.1:-77281 None:intergenic
CGACCTAGAATGAGAAGTGA+TGG 0.501558 1.1:+78707 MS.gene27114:CDS
TAAGAATCTCAACAACTTCA+GGG 0.504579 1.1:-78427 None:intergenic
AATATAAACTAAACTATGGA+AGG 0.504836 1.1:+78882 MS.gene27114:CDS
GTGTGTCGGCTTAAAATTCG+TGG 0.509079 1.1:+78452 MS.gene27114:CDS
GATGGTTATACTTGGAAGAG+AGG 0.511922 1.1:+78812 MS.gene27114:CDS
CCTCACTCGCGATGATCGAA+TGG 0.514018 1.1:+77286 MS.gene27114:CDS
GGGTGGTTGGAGACTGAGAT+GGG 0.516205 1.1:+78728 MS.gene27114:CDS
GTGGGAGCGTTGGCGAGGGA+AGG 0.519021 1.1:-77224 None:intergenic
GGCGGCAGAACGGAAAGTCT+TGG 0.522635 1.1:-77129 None:intergenic
GAATTATCGTTTGGTGTAGC+AGG 0.528065 1.1:+78578 MS.gene27114:CDS
TTGAGATTCTTAATGTGTGT+CGG 0.528788 1.1:+78438 MS.gene27114:CDS
TAGATCTCTCTCTTTAGGCT+GGG 0.531709 1.1:+77440 MS.gene27114:CDS
AACTTATCTTGTGTTCAAGA+TGG 0.537321 1.1:+78517 MS.gene27114:CDS
GGATAAAGGATGGTTATACT+TGG 0.541442 1.1:+78804 MS.gene27114:CDS
ATCGGCTATGAGTTGGGGAT+CGG 0.541544 1.1:-77186 None:intergenic
AGTATTCGGGGACAAGGCAC+GGG 0.541568 1.1:-78486 None:intergenic
ATTTATGCAGAGCGTTCAGT+TGG 0.543756 1.1:+77380 MS.gene27114:intron
AGTATTACTGGGATAAAGGA+TGG 0.544593 1.1:+78794 MS.gene27114:CDS
TAGTATTCGGGGACAAGGCA+CGG 0.547315 1.1:-78487 None:intergenic
TCTCTCTCTTTAGGCTGGGC+CGG 0.548467 1.1:+77444 MS.gene27114:CDS
GAGGATAGGATGATTGGAGA+GGG 0.548862 1.1:-77267 None:intergenic
AAAATCTTCCCAAGCAACGC+CGG 0.549348 1.1:-77463 None:intergenic
AATGAGAAGTGATGGGTGGT+TGG 0.556049 1.1:+78715 MS.gene27114:CDS
AGGAGTCGTACGACAGAGTA+CGG 0.558822 1.1:-77078 None:intergenic
TAGATCCAAACGTGAAATGT+AGG 0.559418 1.1:+78633 MS.gene27114:CDS
GGGAGCGTTTGAAGAATTGG+CGG 0.565646 1.1:+77040 MS.gene27114:CDS
ATCCCAGATTTCATCGGAGT+CGG 0.571899 1.1:-77150 None:intergenic
CCATTCGATCATCGCGAGTG+AGG 0.573985 1.1:-77286 None:intergenic
AGATGATATCGGCTATGAGT+TGG 0.577378 1.1:-77193 None:intergenic
CATCGGAGTCGGCGGCAGAA+CGG 0.577973 1.1:-77139 None:intergenic
TTTGGTGGGAGCGTTGGCGA+GGG 0.580470 1.1:-77228 None:intergenic
TCGTAACTGGACTTCTATGT+CGG 0.583353 1.1:+77494 MS.gene27114:CDS
GCGTTTGAAGAATTGGCGGA+AGG 0.584683 1.1:+77044 MS.gene27114:CDS
TGGGTGGTTGGAGACTGAGA+TGG 0.588372 1.1:+78727 MS.gene27114:CDS
GAAGGGTCTTGTGTCTTGGA+TGG 0.589989 1.1:+78900 MS.gene27114:CDS
AGGAATAGAAGTTAGGCCAA+AGG 0.594471 1.1:+78847 MS.gene27114:CDS
ATGATATCGGCTATGAGTTG+GGG 0.603766 1.1:-77191 None:intergenic
AAGTCCAAATGAGTATTACT+GGG 0.610733 1.1:+78783 MS.gene27114:CDS
ATATGTAGTATTCGGGGACA+AGG 0.615096 1.1:-78492 None:intergenic
AAAACGATCCCAGATTTCAT+CGG 0.615581 1.1:-77156 None:intergenic
ACACCAAACGATAATTCCAC+AGG 0.625699 1.1:-78572 None:intergenic
ATATAAACTAAACTATGGAA+GGG 0.626520 1.1:+78883 MS.gene27114:CDS
TTATCGTTTGGTGTAGCAGG+TGG 0.634867 1.1:+78581 MS.gene27114:CDS
GTTCAGTTGGATAGAAAGAG+TGG 0.637141 1.1:+77393 MS.gene27114:CDS
CCAGATTTCATCGGAGTCGG+CGG 0.638496 1.1:-77147 None:intergenic
GACCTAGAATGAGAAGTGAT+GGG 0.647775 1.1:+78708 MS.gene27114:CDS
TGAGGATAGGATGATTGGAG+AGG 0.662214 1.1:-77268 None:intergenic
TGGGGTGAAGAACTATCCTG+TGG 0.664342 1.1:+78556 MS.gene27114:CDS
ATCCAAACGTGAAATGTAGG+CGG 0.671536 1.1:+78636 MS.gene27114:CDS
AGTTGCATATGTAGTATTCG+GGG 0.713654 1.1:-78498 None:intergenic
CTAGAATGAGAAGTGATGGG+TGG 0.757147 1.1:+78711 MS.gene27114:CDS
CGTACGACTCCTGTTGACGT+CGG 0.771940 1.1:+77089 MS.gene27114:CDS
AGTCGTACGACAGAGTACGG+CGG 0.815749 1.1:-77075 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GGGGGGGGGGGGGGGGGGGG+TGG - chr1.1:77919-77938 None:intergenic 100.0%
!! AATCAATATAAACTAAACTA+TGG + chr1.1:78878-78897 MS.gene27114:CDS 15.0%
!! TAAGTAAAAAAATGAATGTT+AGG - chr1.1:77767-77786 None:intergenic 15.0%
!! TTGAATAAAGATAGATAAAT+TGG - chr1.1:77336-77355 None:intergenic 15.0%
!!! TTTGTTTTGATTCAAAATTT+GGG + chr1.1:78017-78036 MS.gene27114:intron 15.0%
!!! TTTTGTTTTGATTCAAAATT+TGG + chr1.1:78016-78035 MS.gene27114:intron 15.0%
!! AATATAAACTAAACTATGGA+AGG + chr1.1:78882-78901 MS.gene27114:CDS 20.0%
!! ATATAAACTAAACTATGGAA+GGG + chr1.1:78883-78902 MS.gene27114:CDS 20.0%
!! ATGCAAATATTCTAAACTAT+GGG - chr1.1:78178-78197 None:intergenic 20.0%
!!! GTTTTTTGTATTCTTATCTT+GGG + chr1.1:78374-78393 MS.gene27114:intron 20.0%
!!! TAATTTTTGTGCACAAATTT+TGG + chr1.1:78397-78416 MS.gene27114:intron 20.0%
!!! TATTACGTTATTTTATCTAC+CGG + chr1.1:77695-77714 MS.gene27114:intron 20.0%
!!! TATTGATTATTATCTTCCTT+TGG - chr1.1:78866-78885 None:intergenic 20.0%
! CACATAATAGAAAAGTTAAG+AGG - chr1.1:77531-77550 None:intergenic 25.0%
! GATGCAAATATTCTAAACTA+TGG - chr1.1:78179-78198 None:intergenic 25.0%
! GGTAGATAAAATAACGTAAT+AGG - chr1.1:77696-77715 None:intergenic 25.0%
! TAAGTTGCATATGTAGTATT+CGG - chr1.1:78503-78522 None:intergenic 25.0%
! TTTAACACCCTTTAGATTTA+TGG + chr1.1:78342-78361 MS.gene27114:intron 25.0%
!! ATCTACCAAAATGACAATTT+TGG - chr1.1:78618-78637 None:intergenic 25.0%
!! TTTTGTTACACAAACCTTTT+TGG + chr1.1:78057-78076 MS.gene27114:intron 25.0%
!!! CATGCTTACTCATTTAATTT+TGG - chr1.1:78240-78259 None:intergenic 25.0%
!!! GGTTTTTTGTATTCTTATCT+TGG + chr1.1:78373-78392 MS.gene27114:intron 25.0%
!!! TAGAACCAAAATTGTCATTT+TGG + chr1.1:78610-78629 MS.gene27114:CDS 25.0%
AACTTATCTTGTGTTCAAGA+TGG + chr1.1:78517-78536 MS.gene27114:CDS 30.0%
AAGTCCAAATGAGTATTACT+GGG + chr1.1:78783-78802 MS.gene27114:CDS 30.0%
AAGTTGCATATGTAGTATTC+GGG - chr1.1:78502-78521 None:intergenic 30.0%
AATGAGTATTACTGGGATAA+AGG + chr1.1:78790-78809 MS.gene27114:CDS 30.0%
AGTGAAAGCCATAAATCTAA+AGG - chr1.1:78353-78372 None:intergenic 30.0%
ATGATGCTAGATTCAAATCA+TGG - chr1.1:77604-77623 None:intergenic 30.0%
CAACAAAATATTAGGTAGTC+AGG + chr1.1:78661-78680 MS.gene27114:CDS 30.0%
GTGAAAGCCATAAATCTAAA+GGG - chr1.1:78352-78371 None:intergenic 30.0%
GTTAGGAAATTGTGAATTCA+TGG - chr1.1:77750-77769 None:intergenic 30.0%
TAAGAATCTCAACAACTTCA+GGG - chr1.1:78430-78449 None:intergenic 30.0%
TCCTAGAATGTTTGATGAAA+AGG + chr1.1:78084-78103 MS.gene27114:intron 30.0%
TTAAGAATCTCAACAACTTC+AGG - chr1.1:78431-78450 None:intergenic 30.0%
TTATCCCAGTAATACTCATT+TGG - chr1.1:78790-78809 None:intergenic 30.0%
TTGAGATTCTTAATGTGTGT+CGG + chr1.1:78438-78457 MS.gene27114:CDS 30.0%
TTGTTGAAGGAATAGAAGTT+AGG + chr1.1:78840-78859 MS.gene27114:CDS 30.0%
TTTAGATTTATGGCTTTCAC+TGG + chr1.1:78352-78371 MS.gene27114:intron 30.0%
! ATCAGTTTGAAGTTGAATGA+AGG - chr1.1:77640-77659 None:intergenic 30.0%
! ATTTTGAGCAAAATCGTAAC+TGG + chr1.1:77481-77500 MS.gene27114:CDS 30.0%
! TAAAGTATCACAATGTACTC+AGG + chr1.1:77796-77815 MS.gene27114:intron 30.0%
! TCCTTTTCATCAAACATTCT+AGG - chr1.1:78088-78107 None:intergenic 30.0%
AAAACGATCCCAGATTTCAT+CGG - chr1.1:77159-77178 None:intergenic 35.0%
AACACACACACACACAATAA+AGG - chr1.1:78121-78140 None:intergenic 35.0%
AAGAGAGGTTTCTTTGTTGA+AGG + chr1.1:78827-78846 MS.gene27114:CDS 35.0%
AGAAAAGTTAAGAGGAAACC+TGG - chr1.1:77523-77542 None:intergenic 35.0%
AGGAAACTGTGAGATGATAT+CGG - chr1.1:77207-77226 None:intergenic 35.0%
AGGCGGATCAACAAAATATT+AGG + chr1.1:78653-78672 MS.gene27114:CDS 35.0%
AGTATTACTGGGATAAAGGA+TGG + chr1.1:78794-78813 MS.gene27114:CDS 35.0%
AGTTGCATATGTAGTATTCG+GGG - chr1.1:78501-78520 None:intergenic 35.0%
ATTGTGTGTGTGTGTGTTTA+GGG + chr1.1:78123-78142 MS.gene27114:intron 35.0%
CAATACATATGCAATGCTTC+GGG - chr1.1:78205-78224 None:intergenic 35.0%
CCAATACATATGCAATGCTT+CGG - chr1.1:78206-78225 None:intergenic 35.0%
GAAGTCCAAATGAGTATTAC+TGG + chr1.1:78782-78801 MS.gene27114:CDS 35.0%
GGATAAAGGATGGTTATACT+TGG + chr1.1:78804-78823 MS.gene27114:CDS 35.0%
TAGATCCAAACGTGAAATGT+AGG + chr1.1:78633-78652 MS.gene27114:CDS 35.0%
TATCCTGTGGAATTATCGTT+TGG + chr1.1:78569-78588 MS.gene27114:CDS 35.0%
TATTGTGTGTGTGTGTGTTT+AGG + chr1.1:78122-78141 MS.gene27114:intron 35.0%
TGTAGGCACTTGAACAAAAA+GGG - chr1.1:77941-77960 None:intergenic 35.0%
TTGAAGTTGAATGAAGGAAC+TGG - chr1.1:77634-77653 None:intergenic 35.0%
! AGATAGAATGCTCTTTTGGT+GGG - chr1.1:77245-77264 None:intergenic 35.0%
! TTTGTGCACAAATTTTGGAC+AGG + chr1.1:78402-78421 MS.gene27114:intron 35.0%
!! AAACTTGTTAGAAAGCACTG+AGG - chr1.1:77999-78018 None:intergenic 35.0%
!! AACTTGTTAGAAAGCACTGA+GGG - chr1.1:77998-78017 None:intergenic 35.0%
ACACCAAACGATAATTCCAC+AGG - chr1.1:78575-78594 None:intergenic 40.0%
AGGAATAGAAGTTAGGCCAA+AGG + chr1.1:78847-78866 MS.gene27114:CDS 40.0%
ATATGTAGTATTCGGGGACA+AGG - chr1.1:78495-78514 None:intergenic 40.0%
ATCCAAACGTGAAATGTAGG+CGG + chr1.1:78636-78655 MS.gene27114:CDS 40.0%
ATGGGAGAGTTCTTCAATTC+AGG + chr1.1:78746-78765 MS.gene27114:CDS 40.0%
ATGGGTCTAAGAAGAACAAG+AGG - chr1.1:78160-78179 None:intergenic 40.0%
CTGTAGGCACTTGAACAAAA+AGG - chr1.1:77942-77961 None:intergenic 40.0%
CTTGTGTTCAAGATGGTTGA+TGG + chr1.1:78524-78543 MS.gene27114:CDS 40.0%
GACCTAGAATGAGAAGTGAT+GGG + chr1.1:78708-78727 MS.gene27114:CDS 40.0%
GATGGTTATACTTGGAAGAG+AGG + chr1.1:78812-78831 MS.gene27114:CDS 40.0%
GGCATAAACTTAGTAGCCTT+AGG - chr1.1:77965-77984 None:intergenic 40.0%
GTAGGCACTTGAACAAAAAG+GGG - chr1.1:77940-77959 None:intergenic 40.0%
GTTCAGTTGGATAGAAAGAG+TGG + chr1.1:77393-77412 MS.gene27114:CDS 40.0%
TAGATCTCTCTCTTTAGGCT+GGG + chr1.1:77440-77459 MS.gene27114:CDS 40.0%
TAGGCACTTGAACAAAAAGG+GGG - chr1.1:77939-77958 None:intergenic 40.0%
TCGTAACTGGACTTCTATGT+CGG + chr1.1:77494-77513 MS.gene27114:CDS 40.0%
TGCTGAATGATGAATGGATC+CGG - chr1.1:77717-77736 None:intergenic 40.0%
! AGATGATATCGGCTATGAGT+TGG - chr1.1:77196-77215 None:intergenic 40.0%
! ATGATATCGGCTATGAGTTG+GGG - chr1.1:77194-77213 None:intergenic 40.0%
! ATTTATGCAGAGCGTTCAGT+TGG + chr1.1:77380-77399 MS.gene27114:intron 40.0%
! CATTCTAGGAGAGACCAAAA+AGG - chr1.1:78074-78093 None:intergenic 40.0%
! GAGATAGAATGCTCTTTTGG+TGG - chr1.1:77246-77265 None:intergenic 40.0%
! GATGATATCGGCTATGAGTT+GGG - chr1.1:77195-77214 None:intergenic 40.0%
!! ACTTGTTAGAAAGCACTGAG+GGG - chr1.1:77997-78016 None:intergenic 40.0%
!! CCGAAGCATTGCATATGTAT+TGG + chr1.1:78203-78222 MS.gene27114:intron 40.0%
!! GAATTATCGTTTGGTGTAGC+AGG + chr1.1:78578-78597 MS.gene27114:CDS 40.0%
!! TTCAAGATGGTTGATGGCTT+TGG + chr1.1:78530-78549 MS.gene27114:CDS 40.0%
!!! ATGGTTGATGGCTTTGGTTT+TGG + chr1.1:78536-78555 MS.gene27114:CDS 40.0%
!!! TGGTTGATGGCTTTGGTTTT+GGG + chr1.1:78537-78556 MS.gene27114:CDS 40.0%
AAAATCTTCCCAAGCAACGC+CGG - chr1.1:77466-77485 None:intergenic 45.0%
AGGCACTTGAACAAAAAGGG+GGG - chr1.1:77938-77957 None:intergenic 45.0%
ATCCCAGATTTCATCGGAGT+CGG - chr1.1:77153-77172 None:intergenic 45.0%
ATCCGCCTACATTTCACGTT+TGG - chr1.1:78641-78660 None:intergenic 45.0%
CACCCATCACTTCTCATTCT+AGG - chr1.1:78713-78732 None:intergenic 45.0%
CATTCATCATTCAGCACAGC+AGG + chr1.1:77721-77740 MS.gene27114:intron 45.0%
CGACCTAGAATGAGAAGTGA+TGG + chr1.1:78707-78726 MS.gene27114:CDS 45.0%
CTAGATCTCTCTCTTTAGGC+TGG + chr1.1:77439-77458 MS.gene27114:CDS 45.0%
CTGCTGTGCTGAATGATGAA+TGG - chr1.1:77723-77742 None:intergenic 45.0%
GAGGATAGGATGATTGGAGA+GGG - chr1.1:77270-77289 None:intergenic 45.0%
GCTGCTAGATCTCTCTCTTT+AGG + chr1.1:77435-77454 MS.gene27114:CDS 45.0%
GTACTCAGGAATCCAGAGTT+GGG + chr1.1:77810-77829 MS.gene27114:intron 45.0%
TGAGGATAGGATGATTGGAG+AGG - chr1.1:77271-77290 None:intergenic 45.0%
TGTACTCAGGAATCCAGAGT+TGG + chr1.1:77809-77828 MS.gene27114:intron 45.0%
! ACTTAGTAGCCTTAGGCTGT+AGG - chr1.1:77958-77977 None:intergenic 45.0%
! CTAGAATGAGAAGTGATGGG+TGG + chr1.1:78711-78730 MS.gene27114:CDS 45.0%
! GGCGAGATAGAATGCTCTTT+TGG - chr1.1:77249-77268 None:intergenic 45.0%
! TATGGAAGGGTCTTGTGTCT+TGG + chr1.1:78896-78915 MS.gene27114:CDS 45.0%
!! AATGAGAAGTGATGGGTGGT+TGG + chr1.1:78715-78734 MS.gene27114:CDS 45.0%
!! GTGTGTCGGCTTAAAATTCG+TGG + chr1.1:78452-78471 MS.gene27114:CDS 45.0%
!! TTATCGTTTGGTGTAGCAGG+TGG + chr1.1:78581-78600 MS.gene27114:CDS 45.0%
!!! GGTTGATGGCTTTGGTTTTG+GGG + chr1.1:78538-78557 MS.gene27114:CDS 45.0%
ACTTGAACAAAAAGGGGGGG+GGG - chr1.1:77934-77953 None:intergenic 50.0%
AGGAGTCGTACGACAGAGTA+CGG - chr1.1:77081-77100 None:intergenic 50.0%
CACTTGAACAAAAAGGGGGG+GGG - chr1.1:77935-77954 None:intergenic 50.0%
CAGAGTTGGGCATTTGGCAA+TGG + chr1.1:77823-77842 MS.gene27114:intron 50.0%
CATTGCCAAATGCCCAACTC+TGG - chr1.1:77825-77844 None:intergenic 50.0%
GCACTTGAACAAAAAGGGGG+GGG - chr1.1:77936-77955 None:intergenic 50.0%
GCGAGTGAGGATAGGATGAT+TGG - chr1.1:77276-77295 None:intergenic 50.0%
GCGTTTGAAGAATTGGCGGA+AGG + chr1.1:77044-77063 MS.gene27114:CDS 50.0%
GGAATCCAGAGTTGGGCATT+TGG + chr1.1:77817-77836 MS.gene27114:intron 50.0%
GGACTTCTATGTCGGATTCC+AGG + chr1.1:77502-77521 MS.gene27114:CDS 50.0%
GGCACTTGAACAAAAAGGGG+GGG - chr1.1:77937-77956 None:intergenic 50.0%
GTTCAAGTGCCTACAGCCTA+AGG + chr1.1:77946-77965 MS.gene27114:intron 50.0%
TAGTATTCGGGGACAAGGCA+CGG - chr1.1:78490-78509 None:intergenic 50.0%
TCATCATTCAGCACAGCAGG+TGG + chr1.1:77724-77743 MS.gene27114:intron 50.0%
TGGGGTGAAGAACTATCCTG+TGG + chr1.1:78556-78575 MS.gene27114:CDS 50.0%
! AGCACTGAGGGGTAGAAGAA+GGG - chr1.1:77986-78005 None:intergenic 50.0%
! ATCGGCTATGAGTTGGGGAT+CGG - chr1.1:77189-77208 None:intergenic 50.0%
! GAAGGGTCTTGTGTCTTGGA+TGG + chr1.1:78900-78919 MS.gene27114:CDS 50.0%
!! AAGCACTGAGGGGTAGAAGA+AGG - chr1.1:77987-78006 None:intergenic 50.0%
!! GGGAGCGTTTGAAGAATTGG+CGG + chr1.1:77040-77059 MS.gene27114:CDS 50.0%
AGTATTCGGGGACAAGGCAC+GGG - chr1.1:78489-78508 None:intergenic 55.0%
AGTCGTACGACAGAGTACGG+CGG - chr1.1:77078-77097 None:intergenic 55.0%
CCAGATTTCATCGGAGTCGG+CGG - chr1.1:77150-77169 None:intergenic 55.0%
CCATTCGATCATCGCGAGTG+AGG - chr1.1:77289-77308 None:intergenic 55.0%
CCTCACTCGCGATGATCGAA+TGG + chr1.1:77286-77305 MS.gene27114:CDS 55.0%
CGATCATCGCGAGTGAGGAT+AGG - chr1.1:77284-77303 None:intergenic 55.0%
CGTACGACTCCTGTTGACGT+CGG + chr1.1:77089-77108 MS.gene27114:CDS 55.0%
CTTGAACAAAAAGGGGGGGG+GGG - chr1.1:77933-77952 None:intergenic 55.0%
GGGTGGTTGGAGACTGAGAT+GGG + chr1.1:78728-78747 MS.gene27114:CDS 55.0%
TCTCTCTCTTTAGGCTGGGC+CGG + chr1.1:77444-77463 MS.gene27114:CDS 55.0%
TGGGTGGTTGGAGACTGAGA+TGG + chr1.1:78727-78746 MS.gene27114:CDS 55.0%
TTGAACAAAAAGGGGGGGGG+GGG - chr1.1:77932-77951 None:intergenic 55.0%
!! CGCCGACTCCGATGAAATCT+GGG + chr1.1:77148-77167 MS.gene27114:CDS 55.0%
!! TGCTCTTTTGGTGGGAGCGT+TGG - chr1.1:77237-77256 None:intergenic 55.0%
GGCGGCAGAACGGAAAGTCT+TGG - chr1.1:77132-77151 None:intergenic 60.0%
TGAACAAAAAGGGGGGGGGG+GGG - chr1.1:77931-77950 None:intergenic 60.0%
!! CCGCCGACTCCGATGAAATC+TGG + chr1.1:77147-77166 MS.gene27114:CDS 60.0%
!! TTTGGTGGGAGCGTTGGCGA+GGG - chr1.1:77231-77250 None:intergenic 60.0%
!!! TTTTGGTGGGAGCGTTGGCG+AGG - chr1.1:77232-77251 None:intergenic 60.0%
AACAAAAAGGGGGGGGGGGG+GGG - chr1.1:77929-77948 None:intergenic 65.0%
CATCGGAGTCGGCGGCAGAA+CGG - chr1.1:77142-77161 None:intergenic 65.0%
CGACTCCTGTTGACGTCGGC+AGG + chr1.1:77093-77112 MS.gene27114:CDS 65.0%
GAACAAAAAGGGGGGGGGGG+GGG - chr1.1:77930-77949 None:intergenic 65.0%
GAGAGCCTGCCGACGTCAAC+AGG - chr1.1:77101-77120 None:intergenic 65.0%
ACAAAAAGGGGGGGGGGGGG+GGG - chr1.1:77928-77947 None:intergenic 70.0%
AGGCTGGGCCGGCGTTGCTT+GGG + chr1.1:77455-77474 MS.gene27114:CDS 70.0%
TAGGCTGGGCCGGCGTTGCT+TGG + chr1.1:77454-77473 MS.gene27114:CDS 70.0%
!! GTGGGAGCGTTGGCGAGGGA+AGG - chr1.1:77227-77246 None:intergenic 70.0%
AAAAAGGGGGGGGGGGGGGG+GGG - chr1.1:77926-77945 None:intergenic 75.0%
CAAAAAGGGGGGGGGGGGGG+GGG - chr1.1:77927-77946 None:intergenic 75.0%
! AAAAGGGGGGGGGGGGGGGG+GGG - chr1.1:77925-77944 None:intergenic 80.0%
! AAAGGGGGGGGGGGGGGGGG+GGG - chr1.1:77924-77943 None:intergenic 85.0%
! AAGGGGGGGGGGGGGGGGGG+GGG - chr1.1:77923-77942 None:intergenic 90.0%
! AGGGGGGGGGGGGGGGGGGG+GGG - chr1.1:77922-77941 None:intergenic 95.0%
! GGGGGGGGGGGGGGGGGGGT+GGG - chr1.1:77918-77937 None:intergenic 95.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 77038 78925 77038 ID=MS.gene27114
chr1.1 mRNA 77038 78925 77038 ID=MS.gene27114.t1;Parent=MS.gene27114
chr1.1 exon 77038 77307 77038 ID=MS.gene27114.t1.exon1;Parent=MS.gene27114.t1
chr1.1 CDS 77038 77307 77038 ID=cds.MS.gene27114.t1;Parent=MS.gene27114.t1
chr1.1 exon 77390 77523 77390 ID=MS.gene27114.t1.exon2;Parent=MS.gene27114.t1
chr1.1 CDS 77390 77523 77390 ID=cds.MS.gene27114.t1;Parent=MS.gene27114.t1
chr1.1 exon 78424 78925 78424 ID=MS.gene27114.t1.exon3;Parent=MS.gene27114.t1
chr1.1 CDS 78424 78925 78424 ID=cds.MS.gene27114.t1;Parent=MS.gene27114.t1
Gene Sequence

>MS.gene27114

ATGGGAGCGTTTGAAGAATTGGCGGAAGGTTGCATTGCCGCCGTACTCTGTCGTACGACTCCTGTTGACGTCGGCAGGCTCTCTCTTGTCTCCAAGACTTTCCGTTCTGCCGCCGACTCCGATGAAATCTGGGATCGTTTTCTCTGTTCCGATCCCCAACTCATAGCCGATATCATCTCACAGTTTCCTTCCCTCGCCAACGCTCCCACCAAAAGAGCATTCTATCTCGCCCTCTCCAATCATCCTATCCTCACTCGCGATGATCGAATGGTATATTATTGTATTCTTCTTTCTTCCAATTTATCTATCTTTATTCAAATGTTTATTAATGCTCTTCGATTTATTTATGCAGAGCGTTCAGTTGGATAGAAAGAGTGGAAAAAAGTGTTACATGCTTGCTGCTAGATCTCTCTCTTTAGGCTGGGCCGGCGTTGCTTGGGAAGATTTTGAGCAAAATCGTAACTGGACTTCTATGTCGGATTCCAGGTTTCCTCTTAACTTTTCTATTATGTGTCTTCTTAACTAGTTATAGTATGAAGTTTAAGTAAAAATTTGTCATTCAACCATGATTTGAATCTAGCATCATCATTTGACCAGTTCCTTCATTCAACTTCAAACTGATTGATTTCTTTAAATTCATTGATATATACTTATACCTATTACGTTATTTTATCTACCGGATCCATTCATCATTCAGCACAGCAGGTGGCCATGAATTCACAATTTCCTAACATTCATTTTTTTACTTAAATTTCAAATAAAGTATCACAATGTACTCAGGAATCCAGAGTTGGGCATTTGGCAATGGAAAAAAGCTTTGAATTTTCATTGTTTTGTTGTTTTTCATTTTTGTTTGTTTGTTAGTCATCACTAACTACCCACCCCCCCCCCCCCCCCCCCCCCTTTTTGTTCAAGTGCCTACAGCCTAAGGCTACTAAGTTTATGCCCTTCTTCTACCCCTCAGTGCTTTCTAACAAGTTTTGTTTTGATTCAAAATTTGGGAAAATAGCTTCATTTCATTTTGTTACACAAACCTTTTTGGTCTCTCCTAGAATGTTTGATGAAAAGGACTAGTACTGACCTTTATTGTGTGTGTGTGTGTTTAGGGATACAGAAGTGCCTCTTGTTCTTCTTAGACCCATAGTTTAGAATATTTGCATCTCACCCGAAGCATTGCATATGTATTGGCAATGTTATTGCCAAAATTAAATGAGTAAGCATGTTTAGTTAAATGAGTAAGCATGTGTTCATGAATAAAAAAAAAATCTATTTTTTATTGATATTTTAAATTATTTCAAATAATATTTAACACCCTTTAGATTTATGGCTTTCACTGGTTTTTTGTATTCTTATCTTGGGTAATTTTTGTGCACAAATTTTGGACAGGTTCCCTGAAGTTGTTGAGATTCTTAATGTGTGTCGGCTTAAAATTCGTGGAAAGATAAACACCCGTGCCTTGTCCCCGAATACTACATATGCAACTTATCTTGTGTTCAAGATGGTTGATGGCTTTGGTTTTGGGGTGAAGAACTATCCTGTGGAATTATCGTTTGGTGTAGCAGGTGGTCACAGTAGAACCAAAATTGTCATTTTGGTAGATCCAAACGTGAAATGTAGGCGGATCAACAAAATATTAGGTAGTCAGGACAACAACGCGTTTAGATTGCAACGACCTAGAATGAGAAGTGATGGGTGGTTGGAGACTGAGATGGGAGAGTTCTTCAATTCAGGCTTAGAAGATGAAGAAGTCCAAATGAGTATTACTGGGATAAAGGATGGTTATACTTGGAAGAGAGGTTTCTTTGTTGAAGGAATAGAAGTTAGGCCAAAGGAAGATAATAATCAATATAAACTAAACTATGGAAGGGTCTTGTGTCTTGGATGGTAG
Protein sequence

>MS.gene27114.t1

MGAFEELAEGCIAAVLCRTTPVDVGRLSLVSKTFRSAADSDEIWDRFLCSDPQLIADIISQFPSLANAPTKRAFYLALSNHPILTRDDRMSVQLDRKSGKKCYMLAARSLSLGWAGVAWEDFEQNRNWTSMSDSRFPEVVEILNVCRLKIRGKINTRALSPNTTYATYLVFKMVDGFGFGVKNYPVELSFGVAGGHSRTKIVILVDPNVKCRRINKILGSQDNNAFRLQRPRMRSDGWLETEMGEFFNSGLEDEEVQMSITGIKDGYTWKRGFFVEGIEVRPKEDNNQYKLNYGRVLCLGW