AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88931

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88931.t1 MTR_1g026510 97.010 301 9 0 1 301 1 301 0.0 605
MS.gene88931.t1 MTR_1g079250 73.754 301 74 1 1 301 1 296 1.13e-161 452
MS.gene88931.t1 MTR_1g079260 63.986 286 92 4 1 286 1 275 6.50e-128 365
MS.gene88931.t1 MTR_1g079180 62.898 283 87 6 1 283 1 265 1.43e-115 334
MS.gene88931.t1 MTR_1g079460 61.888 286 92 5 1 286 1 269 5.86e-113 327
MS.gene88931.t1 MTR_1g079370 58.657 283 99 6 1 283 1 265 1.28e-109 319
MS.gene88931.t1 MTR_1g079200 60.777 283 92 5 1 283 1 264 4.67e-109 317
MS.gene88931.t1 MTR_1g100597 61.246 289 95 8 1 286 1 275 1.49e-108 317
MS.gene88931.t1 MTR_1g079380 55.705 298 113 7 1 297 1 280 3.75e-100 296
MS.gene88931.t1 MTR_3g006850 53.548 310 113 5 2 286 15 318 3.83e-99 294
MS.gene88931.t1 MTR_1g079850 54.949 293 116 6 1 286 1 284 2.85e-97 288
MS.gene88931.t1 MTR_1g079230 53.425 292 114 6 1 286 1 276 3.88e-95 282
MS.gene88931.t1 MTR_3g452600 54.225 284 106 7 4 286 10 270 9.85e-91 271
MS.gene88931.t1 MTR_1g079350 52.962 287 119 7 1 283 1 275 6.08e-90 269
MS.gene88931.t1 MTR_1g100613 56.911 246 90 5 20 260 5 239 3.51e-85 256
MS.gene88931.t1 MTR_3g450990 50.667 300 108 10 4 286 10 286 1.30e-81 248
MS.gene88931.t1 MTR_2g079100 48.789 289 120 7 3 289 8 270 4.50e-77 236
MS.gene88931.t1 MTR_1g079410 46.154 286 132 8 3 284 7 274 4.77e-74 228
MS.gene88931.t1 MTR_7g096300 51.087 276 109 7 1 273 1 253 2.31e-73 239
MS.gene88931.t1 MTR_1g079340 46.230 305 123 9 1 283 40 325 8.88e-71 222
MS.gene88931.t1 MTR_1g079140 41.667 300 149 5 4 283 13 306 3.40e-67 212
MS.gene88931.t1 MTR_1g079400 40.816 294 134 9 4 283 12 279 1.23e-58 189
MS.gene88931.t1 MTR_1g078470 56.383 188 62 6 96 283 17 184 1.54e-56 181
MS.gene88931.t1 MTR_7g112550 38.754 289 152 11 7 284 10 284 3.00e-54 178
MS.gene88931.t1 MTR_1g083580 38.225 293 149 11 4 283 8 281 6.83e-54 177
MS.gene88931.t1 MTR_1g079240 48.705 193 73 5 91 283 13 179 8.49e-46 153
MS.gene88931.t1 MTR_1g079350 59.420 138 49 2 1 136 1 133 1.01e-45 154
MS.gene88931.t1 MTR_7g112560 33.803 284 157 8 4 283 1 257 1.85e-42 147
MS.gene88931.t1 MTR_1g076870 54.745 137 47 4 136 261 2 134 1.04e-39 136
MS.gene88931.t1 MTR_4g023570 31.915 282 145 9 4 283 1 237 6.83e-36 129
MS.gene88931.t1 MTR_2g042540 59.813 107 40 2 1 106 1 105 1.94e-35 125
MS.gene88931.t1 MTR_1g079130 38.974 195 98 4 104 283 1 189 8.05e-34 122
MS.gene88931.t1 MTR_1g078460 60.674 89 32 1 1 89 64 149 1.42e-30 112
MS.gene88931.t1 MTR_7g096310 46.309 149 61 4 58 196 24 163 1.06e-29 111
MS.gene88931.t1 MTR_1g079210 50.794 126 53 4 137 256 7 129 3.56e-27 103
MS.gene88931.t1 MTR_3g058640 48.649 111 46 3 136 246 7 106 1.60e-22 90.5
MS.gene88931.t1 MTR_1g079220 54.545 77 24 3 173 249 1 66 2.73e-17 75.5
MS.gene88931.t1 MTR_1g079390 67.308 52 14 2 227 278 7 55 5.64e-14 65.9
MS.gene88931.t1 MTR_4g083970 27.461 193 102 8 94 283 133 290 5.72e-12 65.5
MS.gene88931.t1 MTR_7g103380 22.776 281 199 7 6 284 21 285 1.52e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88931.t1 AT2G02250 37.993 279 151 7 4 282 47 303 2.45e-55 182
MS.gene88931.t1 AT1G80110 39.350 277 142 6 7 282 4 255 9.48e-55 179
MS.gene88931.t1 AT2G02240 34.982 283 161 7 4 286 61 320 1.07e-52 175
MS.gene88931.t1 AT2G02360 35.943 281 152 6 4 282 14 268 1.54e-52 173
MS.gene88931.t1 AT2G02230 32.808 317 167 8 4 286 32 336 8.91e-50 168
MS.gene88931.t1 AT1G09155 36.678 289 160 9 7 283 3 280 1.43e-46 158
MS.gene88931.t1 AT2G02340 34.138 290 162 8 1 282 33 301 1.32e-45 156
MS.gene88931.t1 AT1G56240 34.722 288 165 8 7 282 4 280 4.92e-45 154
MS.gene88931.t1 AT2G02350 32.192 292 174 6 2 291 22 291 1.77e-44 153
MS.gene88931.t1 AT1G56250 35.294 289 164 8 7 283 4 281 1.59e-43 150
MS.gene88931.t1 AT2G02300 31.034 290 154 10 2 284 32 282 9.36e-37 133
MS.gene88931.t1 AT2G02310 31.095 283 171 7 4 284 45 305 2.08e-36 132
MS.gene88931.t1 AT2G02320 31.119 286 159 10 6 282 42 298 1.30e-34 127
MS.gene88931.t1 AT5G24560 42.208 154 78 3 4 157 3 145 6.87e-30 112
MS.gene88931.t1 AT2G02350 30.000 190 115 5 104 291 1 174 5.85e-20 85.5
MS.gene88931.t1 AT3G53000 23.920 301 182 12 6 290 23 292 4.48e-15 74.7
MS.gene88931.t1 AT1G33920 27.419 186 97 7 103 283 12 164 1.21e-11 62.4
MS.gene88931.t1 AT4G19850 28.426 197 94 8 97 283 17 176 2.09e-11 62.0
MS.gene88931.t1 AT4G19850 29.442 197 92 9 97 283 59 218 2.59e-11 62.8

Find 91 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTACCAAAATGACAATTT+TGG 0.213350 1.3:-14206 None:intergenic
ATGGTTGATGGCTTTGGTTT+TGG 0.220885 1.3:+14127 MS.gene88931:CDS
TAGAACCAAAATTGTCATTT+TGG 0.238661 1.3:+14201 MS.gene88931:CDS
CCGCCGACTCCGATGAAATC+TGG 0.286656 1.3:+12756 MS.gene88931:CDS
GCTGCTAGATCTCTCTCTTT+AGG 0.290498 1.3:+13044 MS.gene88931:CDS
TAGGCTGGGCCGGCGTTGCT+TGG 0.291939 1.3:+13063 MS.gene88931:CDS
ATGGGAGAGTTCTTCAATTC+AGG 0.292340 1.3:+14337 MS.gene88931:CDS
GAAGTCCAAATGAGTATTAC+TGG 0.304926 1.3:+14373 MS.gene88931:CDS
TTAAGAATCTCAACAACTTC+AGG 0.315871 1.3:-14019 None:intergenic
TAAGTTGCATATGTAGTATT+CGG 0.322872 1.3:-14091 None:intergenic
TATTGATTATTATCTTCCTT+TGG 0.333817 1.3:-14454 None:intergenic
TTATCCCAGTAATACTCATT+TGG 0.337010 1.3:-14378 None:intergenic
GAGAGCCTGCCGACGTCAAC+AGG 0.341542 1.3:-12707 None:intergenic
GGCGAGATAGAATGCTCTTT+TGG 0.351997 1.3:-12855 None:intergenic
CACCCATCACTTCTCATTCT+AGG 0.353018 1.3:-14301 None:intergenic
AATGGGAGCGTTTGAAGAAT+TGG 0.354251 1.3:+12646 None:intergenic
GGACTTCTATGTCGGATTCC+AGG 0.358513 1.3:+13111 MS.gene88931:CDS
AATCAATATAAACTAAACTA+TGG 0.359047 1.3:+14469 MS.gene88931:CDS
AATGAGTATTACTGGGATAA+AGG 0.362381 1.3:+14381 MS.gene88931:CDS
ATCCGCCTACATTTCACGTT+TGG 0.365825 1.3:-14229 None:intergenic
AGGCGGATCAACAAAATATT+AGG 0.370152 1.3:+14244 MS.gene88931:CDS
TTCAAGATGGTTGATGGCTT+TGG 0.374603 1.3:+14121 MS.gene88931:CDS
CTAGATCTCTCTCTTTAGGC+TGG 0.381827 1.3:+13048 MS.gene88931:CDS
AAGTTGCATATGTAGTATTC+GGG 0.388989 1.3:-14090 None:intergenic
CTTGTGTTCAAGATGGTTGA+TGG 0.390765 1.3:+14115 MS.gene88931:CDS
TTGTTGAAGGAATAGAAGTT+AGG 0.402827 1.3:+14431 MS.gene88931:CDS
AGGCTGGGCCGGCGTTGCTT+GGG 0.405783 1.3:+13064 MS.gene88931:CDS
CAACAAAATATTAGGTAGTC+AGG 0.424037 1.3:+14252 MS.gene88931:CDS
AAGAGAGGTTTCTTTGTTGA+AGG 0.432696 1.3:+14418 MS.gene88931:CDS
GCGAGTGAGGATAGGATGAT+TGG 0.443568 1.3:-12882 None:intergenic
CGCCGACTCCGATGAAATCT+GGG 0.449269 1.3:+12757 MS.gene88931:CDS
GATGATATCGGCTATGAGTT+GGG 0.450854 1.3:-12801 None:intergenic
TATCCTGTGGAATTATCGTT+TGG 0.462656 1.3:+14160 MS.gene88931:CDS
CGACTCCTGTTGACGTCGGC+AGG 0.489769 1.3:+12702 MS.gene88931:CDS
CCTCACTCGCGATGGTCGAA+TGG 0.491341 1.3:+12895 MS.gene88931:CDS
TATGGAAGGGTCTTGTGTCT+TGG 0.493873 1.3:+14487 MS.gene88931:CDS
AGGAAACTGTGAGATGATAT+CGG 0.495713 1.3:-12813 None:intergenic
CGACCTAGAATGAGAAGTGA+TGG 0.501558 1.3:+14298 MS.gene88931:CDS
TAAGAATCTCAACAACTTCA+GGG 0.504579 1.3:-14018 None:intergenic
AATATAAACTAAACTATGGA+AGG 0.504836 1.3:+14473 MS.gene88931:CDS
CGACCATCGCGAGTGAGGAT+AGG 0.506878 1.3:-12890 None:intergenic
GTGTGTCGGCTTAAAATTCG+TGG 0.509079 1.3:+14043 MS.gene88931:CDS
GATGGTTATACTTGGAAGAG+AGG 0.511922 1.3:+14403 MS.gene88931:CDS
GGGTGGTTGGAGACTGAGAT+GGG 0.516205 1.3:+14319 MS.gene88931:CDS
GTGGGAGCGTTGGCGAGGGA+AGG 0.519021 1.3:-12833 None:intergenic
GGCGGCAGAACGGAAAGTCT+TGG 0.522635 1.3:-12738 None:intergenic
GAATTATCGTTTGGTGTAGC+AGG 0.528065 1.3:+14169 MS.gene88931:CDS
TTGAGATTCTTAATGTGTGT+CGG 0.528788 1.3:+14029 MS.gene88931:CDS
TAGATCTCTCTCTTTAGGCT+GGG 0.531709 1.3:+13049 MS.gene88931:CDS
AACTTATCTTGTGTTCAAGA+TGG 0.537321 1.3:+14108 MS.gene88931:CDS
GGATAAAGGATGGTTATACT+TGG 0.541442 1.3:+14395 MS.gene88931:CDS
ATCGGCTATGAGTTGGGGAT+CGG 0.541544 1.3:-12795 None:intergenic
AGTATTCGGGGACAAGGCAC+GGG 0.541568 1.3:-14077 None:intergenic
ATTTATGCAGAGCGTTCAGT+TGG 0.543756 1.3:+12989 MS.gene88931:intron
AGTATTACTGGGATAAAGGA+TGG 0.544593 1.3:+14385 MS.gene88931:CDS
TAGTATTCGGGGACAAGGCA+CGG 0.547315 1.3:-14078 None:intergenic
TCTCTCTCTTTAGGCTGGGC+CGG 0.548467 1.3:+13053 MS.gene88931:CDS
GAGGATAGGATGATTGGAGA+GGG 0.548862 1.3:-12876 None:intergenic
AAAATCTTCCCAAGCAACGC+CGG 0.549348 1.3:-13072 None:intergenic
AATGAGAAGTGATGGGTGGT+TGG 0.556049 1.3:+14306 MS.gene88931:CDS
AGGAGTCGTACGACAGAGTA+CGG 0.558822 1.3:-12687 None:intergenic
TAGATCCAAACGTGAAATGT+AGG 0.559418 1.3:+14224 MS.gene88931:CDS
GGGAGCGTTTGAAGAATTGG+CGG 0.565646 1.3:+12649 MS.gene88931:CDS
ATCCCAGATTTCATCGGAGT+CGG 0.571899 1.3:-12759 None:intergenic
AGATGATATCGGCTATGAGT+TGG 0.577378 1.3:-12802 None:intergenic
CATCGGAGTCGGCGGCAGAA+CGG 0.577973 1.3:-12748 None:intergenic
CCATTCGACCATCGCGAGTG+AGG 0.580123 1.3:-12895 None:intergenic
TTTGGTGGGAGCGTTGGCGA+GGG 0.580470 1.3:-12837 None:intergenic
TCGTAACTGGACTTCTATGT+CGG 0.583353 1.3:+13103 MS.gene88931:CDS
GCGTTTGAAGAATTGGCGGA+AGG 0.584683 1.3:+12653 MS.gene88931:CDS
TGGGTGGTTGGAGACTGAGA+TGG 0.588372 1.3:+14318 MS.gene88931:CDS
GAAGGGTCTTGTGTCTTGGA+TGG 0.589989 1.3:+14491 MS.gene88931:CDS
AGGAATAGAAGTTAGGCCAA+AGG 0.594471 1.3:+14438 MS.gene88931:CDS
ATGATATCGGCTATGAGTTG+GGG 0.603766 1.3:-12800 None:intergenic
AAGTCCAAATGAGTATTACT+GGG 0.610733 1.3:+14374 MS.gene88931:CDS
ATATGTAGTATTCGGGGACA+AGG 0.615096 1.3:-14083 None:intergenic
AAAACGATCCCAGATTTCAT+CGG 0.615581 1.3:-12765 None:intergenic
ACACCAAACGATAATTCCAC+AGG 0.625699 1.3:-14163 None:intergenic
ATATAAACTAAACTATGGAA+GGG 0.626520 1.3:+14474 MS.gene88931:CDS
CATCCTATCCTCACTCGCGA+TGG 0.630150 1.3:+12887 MS.gene88931:CDS
TTATCGTTTGGTGTAGCAGG+TGG 0.634867 1.3:+14172 MS.gene88931:CDS
GTTCAGTTGGATAGAAAGAG+TGG 0.637141 1.3:+13002 MS.gene88931:CDS
CCAGATTTCATCGGAGTCGG+CGG 0.638496 1.3:-12756 None:intergenic
GACCTAGAATGAGAAGTGAT+GGG 0.647775 1.3:+14299 MS.gene88931:CDS
TGAGGATAGGATGATTGGAG+AGG 0.662214 1.3:-12877 None:intergenic
TGGGGTGAAGAACTATCCTG+TGG 0.664342 1.3:+14147 MS.gene88931:CDS
ATCCAAACGTGAAATGTAGG+CGG 0.671536 1.3:+14227 MS.gene88931:CDS
AGTTGCATATGTAGTATTCG+GGG 0.713654 1.3:-14089 None:intergenic
CTAGAATGAGAAGTGATGGG+TGG 0.757147 1.3:+14302 MS.gene88931:CDS
CGTACGACTCCTGTTGACGT+CGG 0.771940 1.3:+12698 MS.gene88931:CDS
AGTCGTACGACAGAGTACGG+CGG 0.815749 1.3:-12684 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAATATAAACTAAACTA+TGG + chr1.3:14469-14488 MS.gene88931:CDS 15.0%
!! TAAGTAAAAAAATGAATGTT+AGG - chr1.3:13376-13395 None:intergenic 15.0%
!! TTGAATAAAGATAGATAAAT+TGG - chr1.3:12945-12964 None:intergenic 15.0%
!!! TTTGTTTTGATTCAAAATTT+GGG + chr1.3:13612-13631 MS.gene88931:intron 15.0%
!!! TTTTGTTTTGATTCAAAATT+TGG + chr1.3:13611-13630 MS.gene88931:intron 15.0%
!! AATATAAACTAAACTATGGA+AGG + chr1.3:14473-14492 MS.gene88931:CDS 20.0%
!! ATATAAACTAAACTATGGAA+GGG + chr1.3:14474-14493 MS.gene88931:CDS 20.0%
!! ATGCAAATATTCTAAACTAT+GGG - chr1.3:13771-13790 None:intergenic 20.0%
!!! GTTTTTTGTATTCTTATCTT+GGG + chr1.3:13965-13984 MS.gene88931:intron 20.0%
!!! TAATTTTTGTGCACAAATTT+TGG + chr1.3:13988-14007 MS.gene88931:intron 20.0%
!!! TATTACGTTATTTTATCTAC+CGG + chr1.3:13304-13323 MS.gene88931:intron 20.0%
!!! TATTGATTATTATCTTCCTT+TGG - chr1.3:14457-14476 None:intergenic 20.0%
! ATGATGCTATATTCAAATCA+TGG - chr1.3:13213-13232 None:intergenic 25.0%
! CACATAATAGAAAAGTTAAG+AGG - chr1.3:13140-13159 None:intergenic 25.0%
! GATGCAAATATTCTAAACTA+TGG - chr1.3:13772-13791 None:intergenic 25.0%
! GGTAGATAAAATAACGTAAT+AGG - chr1.3:13305-13324 None:intergenic 25.0%
! TAAGTTGCATATGTAGTATT+CGG - chr1.3:14094-14113 None:intergenic 25.0%
! TTTAACACCCTTTAGATTTA+TGG + chr1.3:13933-13952 MS.gene88931:intron 25.0%
!! ATCTACCAAAATGACAATTT+TGG - chr1.3:14209-14228 None:intergenic 25.0%
!! TTTTGTTACACAAACCTTTT+TGG + chr1.3:13652-13671 MS.gene88931:intron 25.0%
!!! CATGCTTACTCATTTAATTT+TGG - chr1.3:13833-13852 None:intergenic 25.0%
!!! GGTTTTTTGTATTCTTATCT+TGG + chr1.3:13964-13983 MS.gene88931:intron 25.0%
!!! TAGAACCAAAATTGTCATTT+TGG + chr1.3:14201-14220 MS.gene88931:CDS 25.0%
AACTTATCTTGTGTTCAAGA+TGG + chr1.3:14108-14127 MS.gene88931:CDS 30.0%
AAGTCCAAATGAGTATTACT+GGG + chr1.3:14374-14393 MS.gene88931:CDS 30.0%
AAGTTGCATATGTAGTATTC+GGG - chr1.3:14093-14112 None:intergenic 30.0%
AATGAGTATTACTGGGATAA+AGG + chr1.3:14381-14400 MS.gene88931:CDS 30.0%
AGTGAAAGCCATAAATCTAA+AGG - chr1.3:13944-13963 None:intergenic 30.0%
CAACAAAATATTAGGTAGTC+AGG + chr1.3:14252-14271 MS.gene88931:CDS 30.0%
GTGAAAGCCATAAATCTAAA+GGG - chr1.3:13943-13962 None:intergenic 30.0%
GTTAGGAAATTGTGAATTCA+TGG - chr1.3:13359-13378 None:intergenic 30.0%
TAAACACACACACACAATAA+AGG - chr1.3:13716-13735 None:intergenic 30.0%
TAAGAATCTCAACAACTTCA+GGG - chr1.3:14021-14040 None:intergenic 30.0%
TCCTAGAATGTTTGATGAAA+AGG + chr1.3:13679-13698 MS.gene88931:intron 30.0%
TTAAGAATCTCAACAACTTC+AGG - chr1.3:14022-14041 None:intergenic 30.0%
TTATCCCAGTAATACTCATT+TGG - chr1.3:14381-14400 None:intergenic 30.0%
TTATTGTGTGTGTGTGTTTA+GGG + chr1.3:13716-13735 MS.gene88931:intron 30.0%
TTGAGATTCTTAATGTGTGT+CGG + chr1.3:14029-14048 MS.gene88931:CDS 30.0%
TTGTTGAAGGAATAGAAGTT+AGG + chr1.3:14431-14450 MS.gene88931:CDS 30.0%
TTTAGATTTATGGCTTTCAC+TGG + chr1.3:13943-13962 MS.gene88931:intron 30.0%
TTTATTGTGTGTGTGTGTTT+AGG + chr1.3:13715-13734 MS.gene88931:intron 30.0%
! ATCAGTTTGAAGTTGAATGA+AGG - chr1.3:13249-13268 None:intergenic 30.0%
! ATTTTGAGCAAAATCGTAAC+TGG + chr1.3:13090-13109 MS.gene88931:CDS 30.0%
! TAAAGTATCACAATGTACTC+AGG + chr1.3:13405-13424 MS.gene88931:intron 30.0%
! TCCTTTTCATCAAACATTCT+AGG - chr1.3:13683-13702 None:intergenic 30.0%
AAAACGATCCCAGATTTCAT+CGG - chr1.3:12768-12787 None:intergenic 35.0%
AAGAGAGGTTTCTTTGTTGA+AGG + chr1.3:14418-14437 MS.gene88931:CDS 35.0%
AGAAAAGTTAAGAGGAAACC+TGG - chr1.3:13132-13151 None:intergenic 35.0%
AGGAAACTGTGAGATGATAT+CGG - chr1.3:12816-12835 None:intergenic 35.0%
AGGCGGATCAACAAAATATT+AGG + chr1.3:14244-14263 MS.gene88931:CDS 35.0%
AGTATTACTGGGATAAAGGA+TGG + chr1.3:14385-14404 MS.gene88931:CDS 35.0%
AGTTGCATATGTAGTATTCG+GGG - chr1.3:14092-14111 None:intergenic 35.0%
CAATACATATGCAATGCTTC+GGG - chr1.3:13798-13817 None:intergenic 35.0%
CCAATACATATGCAATGCTT+CGG - chr1.3:13799-13818 None:intergenic 35.0%
GAAGTCCAAATGAGTATTAC+TGG + chr1.3:14373-14392 MS.gene88931:CDS 35.0%
GGATAAAGGATGGTTATACT+TGG + chr1.3:14395-14414 MS.gene88931:CDS 35.0%
TAGATCCAAACGTGAAATGT+AGG + chr1.3:14224-14243 MS.gene88931:CDS 35.0%
TATCCTGTGGAATTATCGTT+TGG + chr1.3:14160-14179 MS.gene88931:CDS 35.0%
TGTAGGCACTTGAACAAAAA+GGG - chr1.3:13536-13555 None:intergenic 35.0%
TTGAAGTTGAATGAAGGAAC+TGG - chr1.3:13243-13262 None:intergenic 35.0%
! AGATAGAATGCTCTTTTGGT+GGG - chr1.3:12854-12873 None:intergenic 35.0%
! TTTGTGCACAAATTTTGGAC+AGG + chr1.3:13993-14012 MS.gene88931:intron 35.0%
!! AAACTTGTTAGAAAGCACTG+AGG - chr1.3:13594-13613 None:intergenic 35.0%
!! AACTTGTTAGAAAGCACTGA+GGG - chr1.3:13593-13612 None:intergenic 35.0%
ACACCAAACGATAATTCCAC+AGG - chr1.3:14166-14185 None:intergenic 40.0%
AGGAATAGAAGTTAGGCCAA+AGG + chr1.3:14438-14457 MS.gene88931:CDS 40.0%
ATATGTAGTATTCGGGGACA+AGG - chr1.3:14086-14105 None:intergenic 40.0%
ATCCAAACGTGAAATGTAGG+CGG + chr1.3:14227-14246 MS.gene88931:CDS 40.0%
ATGGGAGAGTTCTTCAATTC+AGG + chr1.3:14337-14356 MS.gene88931:CDS 40.0%
ATGGGTCTAAGAAGAACAAG+AGG - chr1.3:13753-13772 None:intergenic 40.0%
CTGTAGGCACTTGAACAAAA+AGG - chr1.3:13537-13556 None:intergenic 40.0%
CTTGTGTTCAAGATGGTTGA+TGG + chr1.3:14115-14134 MS.gene88931:CDS 40.0%
GACCTAGAATGAGAAGTGAT+GGG + chr1.3:14299-14318 MS.gene88931:CDS 40.0%
GATGGTTATACTTGGAAGAG+AGG + chr1.3:14403-14422 MS.gene88931:CDS 40.0%
GGCATAAACTTAGTAGCCTT+AGG - chr1.3:13560-13579 None:intergenic 40.0%
GTAGGCACTTGAACAAAAAG+GGG - chr1.3:13535-13554 None:intergenic 40.0%
GTTCAGTTGGATAGAAAGAG+TGG + chr1.3:13002-13021 MS.gene88931:CDS 40.0%
TAGATCTCTCTCTTTAGGCT+GGG + chr1.3:13049-13068 MS.gene88931:CDS 40.0%
TAGGCACTTGAACAAAAAGG+GGG - chr1.3:13534-13553 None:intergenic 40.0%
TCGTAACTGGACTTCTATGT+CGG + chr1.3:13103-13122 MS.gene88931:CDS 40.0%
TGCTGAATGATGAATGGATC+CGG - chr1.3:13326-13345 None:intergenic 40.0%
! AGATGATATCGGCTATGAGT+TGG - chr1.3:12805-12824 None:intergenic 40.0%
! ATGATATCGGCTATGAGTTG+GGG - chr1.3:12803-12822 None:intergenic 40.0%
! ATTTATGCAGAGCGTTCAGT+TGG + chr1.3:12989-13008 MS.gene88931:intron 40.0%
! CATTCTAGGAGAGACCAAAA+AGG - chr1.3:13669-13688 None:intergenic 40.0%
! GAGATAGAATGCTCTTTTGG+TGG - chr1.3:12855-12874 None:intergenic 40.0%
! GATGATATCGGCTATGAGTT+GGG - chr1.3:12804-12823 None:intergenic 40.0%
!! ACTTGTTAGAAAGCACTGAG+GGG - chr1.3:13592-13611 None:intergenic 40.0%
!! CCGAAGCATTGCATATGTAT+TGG + chr1.3:13796-13815 MS.gene88931:intron 40.0%
!! GAATTATCGTTTGGTGTAGC+AGG + chr1.3:14169-14188 MS.gene88931:CDS 40.0%
!! TTCAAGATGGTTGATGGCTT+TGG + chr1.3:14121-14140 MS.gene88931:CDS 40.0%
!!! ATGGTTGATGGCTTTGGTTT+TGG + chr1.3:14127-14146 MS.gene88931:CDS 40.0%
!!! TGGTTGATGGCTTTGGTTTT+GGG + chr1.3:14128-14147 MS.gene88931:CDS 40.0%
AAAATCTTCCCAAGCAACGC+CGG - chr1.3:13075-13094 None:intergenic 45.0%
AGGCACTTGAACAAAAAGGG+GGG - chr1.3:13533-13552 None:intergenic 45.0%
ATCCCAGATTTCATCGGAGT+CGG - chr1.3:12762-12781 None:intergenic 45.0%
ATCCGCCTACATTTCACGTT+TGG - chr1.3:14232-14251 None:intergenic 45.0%
CACCCATCACTTCTCATTCT+AGG - chr1.3:14304-14323 None:intergenic 45.0%
CATTCATCATTCAGCACAGC+AGG + chr1.3:13330-13349 MS.gene88931:intron 45.0%
CGACCTAGAATGAGAAGTGA+TGG + chr1.3:14298-14317 MS.gene88931:CDS 45.0%
CTAGATCTCTCTCTTTAGGC+TGG + chr1.3:13048-13067 MS.gene88931:CDS 45.0%
CTGCTGTGCTGAATGATGAA+TGG - chr1.3:13332-13351 None:intergenic 45.0%
GAGGATAGGATGATTGGAGA+GGG - chr1.3:12879-12898 None:intergenic 45.0%
GCTGCTAGATCTCTCTCTTT+AGG + chr1.3:13044-13063 MS.gene88931:CDS 45.0%
GTACTCAGGAATCCAGAGTT+GGG + chr1.3:13419-13438 MS.gene88931:intron 45.0%
TGAGGATAGGATGATTGGAG+AGG - chr1.3:12880-12899 None:intergenic 45.0%
TGTACTCAGGAATCCAGAGT+TGG + chr1.3:13418-13437 MS.gene88931:intron 45.0%
! ACTTAGTAGCCTTAGGCTGT+AGG - chr1.3:13553-13572 None:intergenic 45.0%
! CTAGAATGAGAAGTGATGGG+TGG + chr1.3:14302-14321 MS.gene88931:CDS 45.0%
! GGCGAGATAGAATGCTCTTT+TGG - chr1.3:12858-12877 None:intergenic 45.0%
! TATGGAAGGGTCTTGTGTCT+TGG + chr1.3:14487-14506 MS.gene88931:CDS 45.0%
!! AATGAGAAGTGATGGGTGGT+TGG + chr1.3:14306-14325 MS.gene88931:CDS 45.0%
!! GTGTGTCGGCTTAAAATTCG+TGG + chr1.3:14043-14062 MS.gene88931:CDS 45.0%
!! TTATCGTTTGGTGTAGCAGG+TGG + chr1.3:14172-14191 MS.gene88931:CDS 45.0%
!!! GGTTGATGGCTTTGGTTTTG+GGG + chr1.3:14129-14148 MS.gene88931:CDS 45.0%
ACTTGAACAAAAAGGGGGGG+GGG - chr1.3:13529-13548 None:intergenic 50.0%
AGGAGTCGTACGACAGAGTA+CGG - chr1.3:12690-12709 None:intergenic 50.0%
CACTTGAACAAAAAGGGGGG+GGG - chr1.3:13530-13549 None:intergenic 50.0%
CAGAGTTGGGCATTTGGCAA+TGG + chr1.3:13432-13451 MS.gene88931:intron 50.0%
CATTGCCAAATGCCCAACTC+TGG - chr1.3:13434-13453 None:intergenic 50.0%
GCACTTGAACAAAAAGGGGG+GGG - chr1.3:13531-13550 None:intergenic 50.0%
GCGAGTGAGGATAGGATGAT+TGG - chr1.3:12885-12904 None:intergenic 50.0%
GCGTTTGAAGAATTGGCGGA+AGG + chr1.3:12653-12672 MS.gene88931:CDS 50.0%
GGAATCCAGAGTTGGGCATT+TGG + chr1.3:13426-13445 MS.gene88931:intron 50.0%
GGACTTCTATGTCGGATTCC+AGG + chr1.3:13111-13130 MS.gene88931:CDS 50.0%
GGCACTTGAACAAAAAGGGG+GGG - chr1.3:13532-13551 None:intergenic 50.0%
GTTCAAGTGCCTACAGCCTA+AGG + chr1.3:13541-13560 MS.gene88931:intron 50.0%
TAGTATTCGGGGACAAGGCA+CGG - chr1.3:14081-14100 None:intergenic 50.0%
TCATCATTCAGCACAGCAGG+TGG + chr1.3:13333-13352 MS.gene88931:intron 50.0%
TGGGGTGAAGAACTATCCTG+TGG + chr1.3:14147-14166 MS.gene88931:CDS 50.0%
! AGCACTGAGGGGTAGAAGAA+GGG - chr1.3:13581-13600 None:intergenic 50.0%
! ATCGGCTATGAGTTGGGGAT+CGG - chr1.3:12798-12817 None:intergenic 50.0%
! GAAGGGTCTTGTGTCTTGGA+TGG + chr1.3:14491-14510 MS.gene88931:CDS 50.0%
!! AAGCACTGAGGGGTAGAAGA+AGG - chr1.3:13582-13601 None:intergenic 50.0%
!! GGGAGCGTTTGAAGAATTGG+CGG + chr1.3:12649-12668 MS.gene88931:CDS 50.0%
AGTATTCGGGGACAAGGCAC+GGG - chr1.3:14080-14099 None:intergenic 55.0%
AGTCGTACGACAGAGTACGG+CGG - chr1.3:12687-12706 None:intergenic 55.0%
CATCCTATCCTCACTCGCGA+TGG + chr1.3:12887-12906 MS.gene88931:CDS 55.0%
CCAGATTTCATCGGAGTCGG+CGG - chr1.3:12759-12778 None:intergenic 55.0%
CGTACGACTCCTGTTGACGT+CGG + chr1.3:12698-12717 MS.gene88931:CDS 55.0%
CTTGAACAAAAAGGGGGGGG+GGG - chr1.3:13528-13547 None:intergenic 55.0%
GGGTGGTTGGAGACTGAGAT+GGG + chr1.3:14319-14338 MS.gene88931:CDS 55.0%
TCTCTCTCTTTAGGCTGGGC+CGG + chr1.3:13053-13072 MS.gene88931:CDS 55.0%
TGGGTGGTTGGAGACTGAGA+TGG + chr1.3:14318-14337 MS.gene88931:CDS 55.0%
!! CGCCGACTCCGATGAAATCT+GGG + chr1.3:12757-12776 MS.gene88931:CDS 55.0%
!! TGCTCTTTTGGTGGGAGCGT+TGG - chr1.3:12846-12865 None:intergenic 55.0%
CCATTCGACCATCGCGAGTG+AGG - chr1.3:12898-12917 None:intergenic 60.0%
CCTCACTCGCGATGGTCGAA+TGG + chr1.3:12895-12914 MS.gene88931:CDS 60.0%
CGACCATCGCGAGTGAGGAT+AGG - chr1.3:12893-12912 None:intergenic 60.0%
GGCGGCAGAACGGAAAGTCT+TGG - chr1.3:12741-12760 None:intergenic 60.0%
!! CCGCCGACTCCGATGAAATC+TGG + chr1.3:12756-12775 MS.gene88931:CDS 60.0%
!! TTTGGTGGGAGCGTTGGCGA+GGG - chr1.3:12840-12859 None:intergenic 60.0%
!!! TTTTGGTGGGAGCGTTGGCG+AGG - chr1.3:12841-12860 None:intergenic 60.0%
CATCGGAGTCGGCGGCAGAA+CGG - chr1.3:12751-12770 None:intergenic 65.0%
CGACTCCTGTTGACGTCGGC+AGG + chr1.3:12702-12721 MS.gene88931:CDS 65.0%
GAGAGCCTGCCGACGTCAAC+AGG - chr1.3:12710-12729 None:intergenic 65.0%
AGGCTGGGCCGGCGTTGCTT+GGG + chr1.3:13064-13083 MS.gene88931:CDS 70.0%
TAGGCTGGGCCGGCGTTGCT+TGG + chr1.3:13063-13082 MS.gene88931:CDS 70.0%
!! GTGGGAGCGTTGGCGAGGGA+AGG - chr1.3:12836-12855 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 12647 14516 12647 ID=MS.gene88931
chr1.3 mRNA 12647 14516 12647 ID=MS.gene88931.t1;Parent=MS.gene88931
chr1.3 exon 12647 12916 12647 ID=MS.gene88931.t1.exon1;Parent=MS.gene88931.t1
chr1.3 CDS 12647 12916 12647 ID=cds.MS.gene88931.t1;Parent=MS.gene88931.t1
chr1.3 exon 12999 13132 12999 ID=MS.gene88931.t1.exon2;Parent=MS.gene88931.t1
chr1.3 CDS 12999 13132 12999 ID=cds.MS.gene88931.t1;Parent=MS.gene88931.t1
chr1.3 exon 14015 14516 14015 ID=MS.gene88931.t1.exon3;Parent=MS.gene88931.t1
chr1.3 CDS 14015 14516 14015 ID=cds.MS.gene88931.t1;Parent=MS.gene88931.t1
Gene Sequence

>MS.gene88931

ATGGGAGCGTTTGAAGAATTGGCGGAAGGTTGCATTGCCGCCGTACTCTGTCGTACGACTCCTGTTGACGTCGGCAGGCTCTCTCTTGTCTCCAAGACTTTCCGTTCTGCCGCCGACTCCGATGAAATCTGGGATCGTTTTCTCTGTTCCGATCCCCAACTCATAGCCGATATCATCTCACAGTTTCCTTCCCTCGCCAACGCTCCCACCAAAAGAGCATTCTATCTCGCCCTCTCCAATCATCCTATCCTCACTCGCGATGGTCGAATGGTATATTATTGTATTCTTCTTTCTTCCAATTTATCTATCTTTATTCAAATGTTTATTAATGCTCTTCGATTTATTTATGCAGAGCGTTCAGTTGGATAGAAAGAGTGGAAAAAAGTGTTACATGCTTGCTGCTAGATCTCTCTCTTTAGGCTGGGCCGGCGTTGCTTGGGAAGATTTTGAGCAAAATCGTAACTGGACTTCTATGTCGGATTCCAGGTTTCCTCTTAACTTTTCTATTATGTGTCTTCTTAACTAGTTATAGTATGAAGTTTAAGTAAAAATTTGTCATTCAACCATGATTTGAATATAGCATCATCATTTGACCAGTTCCTTCATTCAACTTCAAACTGATTGATTTCTTTAAATTCATTGATATATACTTATACCTATTACGTTATTTTATCTACCGGATCCATTCATCATTCAGCACAGCAGGTGGCCATGAATTCACAATTTCCTAACATTCATTTTTTTACTTAAATTTCAAATAAAGTATCACAATGTACTCAGGAATCCAGAGTTGGGCATTTGGCAATGGAAAAAAAGCTTTGAATTTTCATTGTTTTGTTGTTTTTCATTTTTGTTTGTTTGTTAGTCATCACTAACTACCCCCCCCCCCTTTTTGTTCAAGTGCCTACAGCCTAAGGCTACTAAGTTTATGCCCTTCTTCTACCCCTCAGTGCTTTCTAACAAGTTTTGTTTTGATTCAAAATTTGGGAAAATAGCTTCATTTCATTTTGTTACACAAACCTTTTTGGTCTCTCCTAGAATGTTTGATGAAAAGGACTAGTACTGACCTTTATTGTGTGTGTGTGTTTAGGGATACAGAAGTGCCTCTTGTTCTTCTTAGACCCATAGTTTAGAATATTTGCATCTCACCCGAAGCATTGCATATGTATTGGCAATGTTATTGCCAAAATTAAATGAGTAAGCATGTTTAGTTAAATGAGTAAGCATGTGTTCATGAATAAAAAAAAATCTATTTTTATTGATATTTTAAATTATTTCAAATAATATTTAACACCCTTTAGATTTATGGCTTTCACTGGTTTTTTGTATTCTTATCTTGGGTAATTTTTGTGCACAAATTTTGGACAGGTTCCCTGAAGTTGTTGAGATTCTTAATGTGTGTCGGCTTAAAATTCGTGGAAAGATAAACACCCGTGCCTTGTCCCCGAATACTACATATGCAACTTATCTTGTGTTCAAGATGGTTGATGGCTTTGGTTTTGGGGTGAAGAACTATCCTGTGGAATTATCGTTTGGTGTAGCAGGTGGTCACAGTAGAACCAAAATTGTCATTTTGGTAGATCCAAACGTGAAATGTAGGCGGATCAACAAAATATTAGGTAGTCAGGACAACAACGCGTTTAGATTGCAACGACCTAGAATGAGAAGTGATGGGTGGTTGGAGACTGAGATGGGAGAGTTCTTCAATTCAGGCTTAGAAGATGAAGAAGTCCAAATGAGTATTACTGGGATAAAGGATGGTTATACTTGGAAGAGAGGTTTCTTTGTTGAAGGAATAGAAGTTAGGCCAAAGGAAGATAATAATCAATATAAACTAAACTATGGAAGGGTCTTGTGTCTTGGATGGTAG
Protein sequence

>MS.gene88931.t1

MGAFEELAEGCIAAVLCRTTPVDVGRLSLVSKTFRSAADSDEIWDRFLCSDPQLIADIISQFPSLANAPTKRAFYLALSNHPILTRDGRMSVQLDRKSGKKCYMLAARSLSLGWAGVAWEDFEQNRNWTSMSDSRFPEVVEILNVCRLKIRGKINTRALSPNTTYATYLVFKMVDGFGFGVKNYPVELSFGVAGGHSRTKIVILVDPNVKCRRINKILGSQDNNAFRLQRPRMRSDGWLETEMGEFFNSGLEDEEVQMSITGIKDGYTWKRGFFVEGIEVRPKEDNNQYKLNYGRVLCLGW