AlfalfaGEDB | MS.gene07250

M. sativa cultivar XinJiangDaYe / MS.gene07250

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00646.36 PF14299.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07250.t1	KEH32832.1	62.3	313	102	7	87	385	8	318	2.20E-92	349.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07250.t1	Q9ZVQ6	39.0	292	140	5	95	383	14	270	9.1e-47	189.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07250.t1	A0A072UT85	62.3	313	102	7	87	385	8	318	1.6e-92	349.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene005023	MS.gene07250	PPI
MS.gene005033	MS.gene07250	PPI
MS.gene07250	MS.gene005027	PPI
MS.gene07250	MS.gene74880	PPI
MS.gene51502	MS.gene07250	PPI
MS.gene07555	MS.gene07250	PPI
MS.gene07250	MS.gene07585	PPI
MS.gene63582	MS.gene07250	PPI
MS.gene07250	MS.gene07571	PPI
MS.gene07250	MS.gene005068	PPI
MS.gene07250	MS.gene51496	PPI
MS.gene49085	MS.gene07250	PPI
MS.gene058615	MS.gene07250	PPI
MS.gene07250	MS.gene49090	PPI
MS.gene67769	MS.gene07250	PPI
MS.gene07572	MS.gene07250	PPI
MS.gene07250	MS.gene005066	PPI
MS.gene07250	MS.gene069492	PPI
MS.gene74877	MS.gene07250	PPI
MS.gene07250	MS.gene95381	PPI
MS.gene88931	MS.gene07250	PPI
MS.gene07580	MS.gene07250	PPI
MS.gene07250	MS.gene24084	PPI
MS.gene95381	MS.gene07250	PPI
MS.gene51487	MS.gene07250	PPI
MS.gene74873	MS.gene07250	PPI
MS.gene49088	MS.gene07250	PPI
MS.gene41433	MS.gene07250	PPI
MS.gene07578	MS.gene07250	PPI
MS.gene74881	MS.gene07250	PPI
MS.gene07250	MS.gene94933	PPI
MS.gene07250	MS.gene96708	PPI
MS.gene74998	MS.gene07250	PPI
MS.gene74913	MS.gene07250	PPI
MS.gene07250	MS.gene058740	PPI
MS.gene07250	MS.gene006296	PPI
MS.gene07559	MS.gene07250	PPI
MS.gene07250	MS.gene005020	PPI
MS.gene07250	MS.gene63582	PPI
MS.gene49186	MS.gene07250	PPI
MS.gene07250	MS.gene49085	PPI
MS.gene74876	MS.gene07250	PPI
MS.gene07571	MS.gene07250	PPI
MS.gene005012	MS.gene07250	PPI
MS.gene43943	MS.gene07250	PPI
MS.gene07585	MS.gene07250	PPI
MS.gene07250	MS.gene34488	PPI
MS.gene41431	MS.gene07250	PPI
MS.gene74860	MS.gene07250	PPI
MS.gene07250	MS.gene04280	PPI
MS.gene52533	MS.gene07250	PPI
MS.gene74863	MS.gene07250	PPI
MS.gene07250	MS.gene000006	PPI
MS.gene07250	MS.gene005013	PPI
MS.gene005019	MS.gene07250	PPI
MS.gene07250	MS.gene27114	PPI
MS.gene49086	MS.gene07250	PPI
MS.gene07250	MS.gene52533	PPI
MS.gene005013	MS.gene07250	PPI
MS.gene07583	MS.gene07250	PPI
MS.gene07250	MS.gene74864	PPI
MS.gene07250	MS.gene07557	PPI
MS.gene07569	MS.gene07250	PPI
MS.gene07250	MS.gene005034	PPI
MS.gene52532	MS.gene07250	PPI
MS.gene07581	MS.gene07250	PPI
MS.gene74872	MS.gene07250	PPI
MS.gene005030	MS.gene07250	PPI
MS.gene005035	MS.gene07250	PPI
MS.gene07250	MS.gene068736	PPI
MS.gene74859	MS.gene07250	PPI
MS.gene07250	MS.gene74858	PPI
MS.gene07250	MS.gene43942	PPI
MS.gene005028	MS.gene07250	PPI
MS.gene52530	MS.gene07250	PPI
MS.gene07250	MS.gene006298	PPI
MS.gene07250	MS.gene49185	PPI
MS.gene74867	MS.gene07250	PPI
MS.gene07250	MS.gene74875	PPI
MS.gene07250	MS.gene005552	PPI
MS.gene07250	MS.gene009359	PPI
MS.gene07250	MS.gene41430	PPI
MS.gene67762	MS.gene07250	PPI
MS.gene07250	MS.gene07556	PPI
MS.gene94933	MS.gene07250	PPI
MS.gene07586	MS.gene07250	PPI
MS.gene34490	MS.gene07250	PPI
MS.gene74912	MS.gene07250	PPI
MS.gene07250	MS.gene005032	PPI
MS.gene07250	MS.gene005033	PPI
MS.gene74878	MS.gene07250	PPI
MS.gene74868	MS.gene07250	PPI
MS.gene059798	MS.gene07250	PPI
MS.gene07556	MS.gene07250	PPI
MS.gene07577	MS.gene07250	PPI
MS.gene000006	MS.gene07250	PPI
MS.gene74911	MS.gene07250	PPI
MS.gene07250	MS.gene006294	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07250.t1	MTR_3g006850	63.107	309	98	7	91	385	12	318	8.78e-122	355
MS.gene07250.t1	MTR_1g079460	60.133	301	81	5	92	385	1	269	6.36e-115	336
MS.gene07250.t1	MTR_1g079180	61.034	290	87	5	92	381	1	264	5.47e-114	333
MS.gene07250.t1	MTR_1g100597	61.149	296	92	6	92	385	1	275	3.87e-113	331
MS.gene07250.t1	MTR_1g079370	58.763	291	92	5	92	381	1	264	4.89e-109	320
MS.gene07250.t1	MTR_1g079260	57.912	297	100	6	92	385	1	275	5.78e-109	321
MS.gene07250.t1	MTR_3g452600	56.757	296	98	4	90	385	5	270	3.60e-107	316
MS.gene07250.t1	MTR_1g079230	59.322	295	100	6	92	385	1	276	3.92e-107	316
MS.gene07250.t1	MTR_1g079200	57.877	292	92	5	92	381	1	263	4.69e-103	305
MS.gene07250.t1	MTR_1g079850	57.288	295	114	4	92	385	1	284	3.00e-101	301
MS.gene07250.t1	MTR_1g026510	53.667	300	119	4	92	385	1	286	1.07e-99	298
MS.gene07250.t1	MTR_1g079250	55.219	297	114	4	92	385	1	281	4.86e-98	294
MS.gene07250.t1	MTR_2g079100	54.362	298	96	6	90	383	4	265	2.76e-95	286
MS.gene07250.t1	MTR_1g079380	55.670	291	101	5	92	381	1	264	2.35e-94	284
MS.gene07250.t1	MTR_1g100613	58.779	262	77	6	111	364	5	243	3.11e-94	282
MS.gene07250.t1	MTR_3g450990	51.603	312	105	7	90	385	5	286	2.47e-93	281
MS.gene07250.t1	MTR_1g079350	52.189	297	112	6	92	381	1	274	1.89e-90	273
MS.gene07250.t1	MTR_1g079410	47.351	302	121	7	90	383	3	274	3.27e-75	234
MS.gene07250.t1	MTR_7g096300	52.500	280	94	6	92	366	1	246	7.90e-74	244
MS.gene07250.t1	MTR_1g079340	45.484	310	128	6	90	381	38	324	1.80e-72	229
MS.gene07250.t1	MTR_1g079140	44.156	308	136	8	95	381	13	305	1.30e-65	211
MS.gene07250.t1	MTR_1g079400	42.395	309	140	7	79	381	2	278	6.46e-62	201
MS.gene07250.t1	MTR_1g079350	64.336	143	51	0	92	234	1	143	1.14e-54	180
MS.gene07250.t1	MTR_1g076870	60.667	150	34	4	224	363	2	136	9.24e-52	169
MS.gene07250.t1	MTR_7g112550	40.956	293	144	10	98	381	10	282	3.97e-51	173
MS.gene07250.t1	MTR_1g078470	50.000	196	68	6	186	381	18	183	1.39e-49	165
MS.gene07250.t1	MTR_1g079240	46.341	205	68	5	181	381	12	178	5.47e-45	153
MS.gene07250.t1	MTR_1g083580	35.789	285	167	6	98	381	11	280	2.56e-44	155
MS.gene07250.t1	MTR_7g112560	36.491	285	148	7	98	381	4	256	7.23e-42	148
MS.gene07250.t1	MTR_2g042540	64.486	107	35	2	92	197	1	105	1.14e-37	132
MS.gene07250.t1	MTR_4g023570	35.088	285	132	6	98	381	4	236	2.87e-37	135
MS.gene07250.t1	MTR_7g096310	51.748	143	55	3	149	281	24	162	2.84e-36	130
MS.gene07250.t1	MTR_1g078460	67.742	93	27	1	89	181	61	150	2.03e-34	125
MS.gene07250.t1	MTR_1g079210	50.360	139	48	2	225	357	7	130	1.28e-32	119
MS.gene07250.t1	MTR_3g058640	48.780	123	40	2	224	346	7	106	6.29e-28	106
MS.gene07250.t1	MTR_1g079130	38.614	202	93	5	195	380	1	187	9.39e-27	105
MS.gene07250.t1	MTR_0113s0040	26.768	198	129	4	97	282	24	217	2.94e-13	70.1
MS.gene07250.t1	MTR_5g038590	46.739	92	43	1	1	86	1	92	9.20e-13	67.4
MS.gene07250.t1	MTR_1g050352	51.807	83	32	3	117	196	46	123	1.08e-12	65.9
MS.gene07250.t1	MTR_1g079220	42.222	90	27	3	261	349	1	66	1.83e-12	62.8
MS.gene07250.t1	MTR_7g103380	23.113	212	147	4	97	296	21	228	2.45e-12	67.4
MS.gene07250.t1	MTR_1g101390	30.822	146	93	5	97	240	30	169	1.09e-11	65.5
MS.gene07250.t1	MTR_1g079190	73.171	41	11	0	334	374	134	174	1.27e-11	63.2
MS.gene07250.t1	MTR_4g102770	29.730	148	97	4	97	242	21	163	1.89e-11	64.7
MS.gene07250.t1	MTR_0002s0020	27.322	183	116	7	83	257	10	183	2.08e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07250.t1	AT2G02360	39.041	292	140	5	95	383	14	270	2.19e-58	191
MS.gene07250.t1	AT1G80110	41.608	286	133	6	98	382	4	256	1.73e-53	178
MS.gene07250.t1	AT2G02250	35.366	328	177	8	58	383	11	305	1.20e-51	175
MS.gene07250.t1	AT2G02340	35.880	301	166	7	85	382	26	302	2.51e-50	171
MS.gene07250.t1	AT2G02240	37.671	292	149	7	95	385	61	320	6.95e-50	171
MS.gene07250.t1	AT2G02230	34.756	328	174	9	86	385	21	336	5.74e-49	169
MS.gene07250.t1	AT2G02350	36.897	290	147	9	98	383	27	284	1.26e-46	161
MS.gene07250.t1	AT1G56240	37.500	296	154	8	98	381	4	280	1.79e-44	155
MS.gene07250.t1	AT2G02320	35.052	291	153	7	97	383	42	300	3.57e-42	150
MS.gene07250.t1	AT1G56250	36.678	289	166	8	98	381	4	280	7.03e-42	149
MS.gene07250.t1	AT1G09155	35.906	298	156	8	98	381	3	279	2.32e-41	147
MS.gene07250.t1	AT2G02310	31.024	332	190	9	55	381	6	303	1.27e-40	146
MS.gene07250.t1	AT2G02300	32.770	296	143	9	94	381	33	280	2.98e-34	128
MS.gene07250.t1	AT5G24560	35.897	195	101	6	95	281	3	181	5.12e-29	112
MS.gene07250.t1	AT2G02350	33.161	193	99	6	195	383	1	167	2.07e-21	90.9
MS.gene07250.t1	AT3G53000	24.026	308	197	9	84	384	10	287	2.83e-14	73.2
MS.gene07250.t1	AT3G61060	28.750	160	106	5	84	240	13	167	2.82e-13	70.1
MS.gene07250.t1	AT1G12710	26.667	240	150	9	97	318	30	261	6.24e-13	69.3
MS.gene07250.t1	AT3G61060	29.375	160	104	6	84	240	13	166	1.79e-12	67.8
MS.gene07250.t1	AT1G63090	26.027	219	132	10	97	295	28	236	6.57e-12	66.2

Find 101 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAAAGAATGGAAGCGTTTA+AGG	0.185860	1.3:-62057601	MS.gene07250:CDS
TAAGCCGCATACTTAGTATT+TGG	0.197924	1.3:+62056267	None:intergenic
GATTGATGCCCGTGGATTTA+AGG	0.214171	1.3:-62056229	MS.gene07250:CDS
TCAGCTTGATCCCCAGAATT+TGG	0.223014	1.3:+62058217	None:intergenic
GTGAAAGGATCGCCAAATTC+TGG	0.223753	1.3:-62058229	MS.gene07250:CDS
AGAGTGTGCCGTTTGTTAAA+AGG	0.297224	1.3:+62058983	None:intergenic
AAGCCGCATACTTAGTATTT+GGG	0.312044	1.3:+62056268	None:intergenic
TAATATTGCTCAATTGTCTT+TGG	0.317767	1.3:+62055867	None:intergenic
TTCTTCAAGGAATTGAAATT+AGG	0.324901	1.3:-62055894	MS.gene07250:CDS
TGCAACTGCTCCCACCAAAA+AGG	0.338766	1.3:-62057400	MS.gene07250:CDS
CGACAAAGCTTAGCAACTTC+AGG	0.342136	1.3:+62056339	None:intergenic
TTGCATTTATGTTTGCTGTT+TGG	0.355251	1.3:+62056093	None:intergenic
CCGCCAATTCCGACATTGTC+TGG	0.356049	1.3:-62057485	MS.gene07250:CDS
TGATTTATTCCGCCGCCTCC+TGG	0.364009	1.3:-62058959	MS.gene07250:intron
TTAAATTAAGAGCTTTCAAT+TGG	0.371567	1.3:-62057203	MS.gene07250:intron
GTCACGCAACAGAGCAAGAT+TGG	0.385718	1.3:-62056049	MS.gene07250:CDS
TTGTGGTGGTATGCCTCCTT+TGG	0.387149	1.3:-62057125	MS.gene07250:CDS
AAGGAAAATTTATTTCTTCA+AGG	0.390874	1.3:-62055907	MS.gene07250:CDS
ATTCCAGACAATGTCGGAAT+TGG	0.394383	1.3:+62057482	None:intergenic
CCATGAGCACGAGCCGAATA+GGG	0.412496	1.3:-62059128	MS.gene07250:CDS
AAATGCAAATGTGCTGCTAC+TGG	0.414241	1.3:-62056078	MS.gene07250:CDS
GGGAGTAGTACGAGAGAGTA+TGG	0.417136	1.3:+62057554	None:intergenic
GGATTCATATGCAAAATTCC+AGG	0.423893	1.3:-62057090	MS.gene07250:intron
ATGGCGGCAATGCACCCTTC+CGG	0.431949	1.3:+62057573	None:intergenic
TATAGACTTCTTCATCTTCG+AGG	0.435124	1.3:+62055962	None:intergenic
CCCTATTCGGCTCGTGCTCA+TGG	0.440574	1.3:+62059128	None:intergenic
GCCATGAGCACGAGCCGAAT+AGG	0.444459	1.3:-62059129	MS.gene07250:CDS
CATCATCGACCATGCTGAAA+AGG	0.449564	1.3:-62057346	MS.gene07250:intron
TGAAAGGATCGCCAAATTCT+GGG	0.452162	1.3:-62058228	MS.gene07250:CDS
TTAGGATGAAATAGAAAGAA+TGG	0.455406	1.3:-62057613	MS.gene07250:intron
ATTGCTCAATTGTCTTTGGC+AGG	0.456017	1.3:+62055871	None:intergenic
CCAGACAATGTCGGAATTGG+CGG	0.468924	1.3:+62057485	None:intergenic
TGTGGTGGTATGCCTCCTTT+GGG	0.469234	1.3:-62057124	MS.gene07250:CDS
GGAGAGCCTGCCGACATCAA+TGG	0.470280	1.3:+62057533	None:intergenic
GGATCTCGGAAATCCCTATT+CGG	0.473572	1.3:+62059115	None:intergenic
CAGCACAAAATTAGTCTGCT+TGG	0.474547	1.3:-62056163	MS.gene07250:CDS
GAGAGCCTGCCGACATCAAT+GGG	0.479310	1.3:+62057534	None:intergenic
TCCGAGATCCTCATTGTAGC+TGG	0.480831	1.3:-62059102	MS.gene07250:CDS
TGTTGATTCAGCTGATGCTC+AGG	0.491002	1.3:-62058185	MS.gene07250:intron
TGTTGATTCCACCGATGCTC+AGG	0.501261	1.3:-62058714	MS.gene07250:intron
GGAAGCGTTTAAGGAATTGC+CGG	0.507940	1.3:-62057592	MS.gene07250:CDS
CTACTCCCATTGATGTCGGC+AGG	0.508684	1.3:-62057539	MS.gene07250:CDS
TGGGTGGTTGGAGATTAAGA+TGG	0.512888	1.3:-62056004	MS.gene07250:CDS
GACAAAGCTTAGCAACTTCA+GGG	0.524349	1.3:+62056340	None:intergenic
TCGTTGTAGTTGCTGGTGAA+AGG	0.527276	1.3:-62058244	MS.gene07250:intron
TCAGCTCCATCCTCGTACTT+CGG	0.527847	1.3:+62058749	None:intergenic
CGTCCTAGACTGAGAATCGA+TGG	0.531049	1.3:-62056024	MS.gene07250:CDS
AGAGTCGATGAATTGAGAAT+CGG	0.532706	1.3:+62057446	None:intergenic
TCACGCAACAGAGCAAGATT+GGG	0.537126	1.3:-62056048	MS.gene07250:CDS
TGTCGGAATTGGCGGCAGAA+AGG	0.538349	1.3:+62057493	None:intergenic
CTCCTTTGGGTGAGAAAGAG+TGG	0.539653	1.3:-62057111	MS.gene07250:CDS
AATTAGTCTGCTTGGAACGT+TGG	0.539900	1.3:-62056155	MS.gene07250:CDS
ATCCTCATTGTAGCTGGAGT+TGG	0.544582	1.3:-62059096	MS.gene07250:intron
ACTGAGAATCGATGGGTGGT+TGG	0.545507	1.3:-62056016	MS.gene07250:CDS
GGGTGGTTGGAGATTAAGAT+GGG	0.557041	1.3:-62056003	MS.gene07250:CDS
GAATATATACCAGGAGGCGG+CGG	0.557659	1.3:+62058950	None:intergenic
TCCAGCTACAATGAGGATCT+CGG	0.564677	1.3:+62059101	None:intergenic
GTGGGAGCAGTTGCAAGGGA+AGG	0.565439	1.3:+62057408	None:intergenic
AAGTACGAGGATGGAGCTGA+TGG	0.571226	1.3:-62058746	MS.gene07250:CDS
GATGGAGCTGATGGTGAAGA+TGG	0.574138	1.3:-62058737	MS.gene07250:CDS
CGTTTAAGGAATTGCCGGAA+GGG	0.574247	1.3:-62057587	MS.gene07250:CDS
TGTTGTCGCTTGAAATTCGT+GGG	0.583600	1.3:-62056314	MS.gene07250:CDS
AGGCGTGTCCTTAAATCCAC+GGG	0.584864	1.3:+62056221	None:intergenic
GAATCACCGAAGTACGAGGA+TGG	0.587645	1.3:-62058755	MS.gene07250:CDS
GCGTTTAAGGAATTGCCGGA+AGG	0.589082	1.3:-62057588	MS.gene07250:CDS
GTGTTGTCGCTTGAAATTCG+TGG	0.589403	1.3:-62056315	MS.gene07250:CDS
AGCCGCATACTTAGTATTTG+GGG	0.590667	1.3:+62056269	None:intergenic
AATGTGCTGCTACTGGGTGG+AGG	0.590947	1.3:-62056071	MS.gene07250:CDS
CAGGCGTGTCCTTAAATCCA+CGG	0.595909	1.3:+62056220	None:intergenic
TCGATGAATTGAGAATCGGC+AGG	0.597831	1.3:+62057450	None:intergenic
ACGCCTGTGGAATTATCTGT+TGG	0.598344	1.3:-62056204	MS.gene07250:CDS
AATGCAAATGTGCTGCTACT+GGG	0.599668	1.3:-62056077	MS.gene07250:CDS
CACCCATCGATTCTCAGTCT+AGG	0.600551	1.3:+62056021	None:intergenic
CGTACTACTCCCATTGATGT+CGG	0.602145	1.3:-62057543	MS.gene07250:CDS
TATGAATATATACCAGGAGG+CGG	0.603326	1.3:+62058947	None:intergenic
TTATCTGTTGGCATCGAAGG+TGG	0.603489	1.3:-62056192	MS.gene07250:CDS
GCTCTTACAATTGATTGTGG+TGG	0.605296	1.3:-62057139	MS.gene07250:CDS
TTTGGTGGGAGCAGTTGCAA+GGG	0.606846	1.3:+62057404	None:intergenic
GAATTATCTGTTGGCATCGA+AGG	0.606957	1.3:-62056195	MS.gene07250:CDS
CACTCTTTCTCACCCAAAGG+AGG	0.607143	1.3:+62057112	None:intergenic
GTTCGTTACCTGAGCATCGG+TGG	0.617690	1.3:+62058706	None:intergenic
ATGCCAACAGATAATTCCAC+AGG	0.618509	1.3:+62056201	None:intergenic
ATACTTAGTATTTGGGGACA+AGG	0.620406	1.3:+62056275	None:intergenic
TGGATTTAAGGACACGCCTG+TGG	0.620629	1.3:-62056217	MS.gene07250:CDS
ATCCACTCTTTCTCACCCAA+AGG	0.620685	1.3:+62057109	None:intergenic
CCAGTTCGTTACCTGAGCAT+CGG	0.623947	1.3:+62058703	None:intergenic
AATTCTGGGGATCAAGCTGA+AGG	0.631926	1.3:-62058214	MS.gene07250:CDS
GTCCCCAAATACTAAGTATG+CGG	0.633956	1.3:-62056271	MS.gene07250:CDS
AGAGCTCTTACAATTGATTG+TGG	0.636596	1.3:-62057142	MS.gene07250:CDS
GTCCTAGACTGAGAATCGAT+GGG	0.647735	1.3:-62056023	MS.gene07250:CDS
GAAAGGATCGCCAAATTCTG+GGG	0.652151	1.3:-62058227	MS.gene07250:CDS
TACCAACTCCAGCTACAATG+AGG	0.652812	1.3:+62059094	None:intergenic
CGATGAATTGAGAATCGGCA+GGG	0.652865	1.3:+62057451	None:intergenic
TTGGCTTCAACGAACAAACG+TGG	0.658388	1.3:+62056112	None:intergenic
TTTAAGATGATTGATGCCCG+TGG	0.665208	1.3:-62056237	MS.gene07250:CDS
GAAAGAATCACCGAAGTACG+AGG	0.675584	1.3:-62058759	MS.gene07250:CDS
TGTGCCGTTTGTTAAAAGGA+TGG	0.677328	1.3:+62058987	None:intergenic
AAAATGATTCCAGACAATGT+CGG	0.677420	1.3:+62057476	None:intergenic
CTAGACTGAGAATCGATGGG+TGG	0.692725	1.3:-62056020	MS.gene07250:CDS
GCAAATGTGCTGCTACTGGG+TGG	0.696344	1.3:-62056074	MS.gene07250:CDS
AGTAGTACGAGAGAGTATGG+CGG	0.791363	1.3:+62057557	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTATCTTTATTTAGATAT+AGG	+	chr1.3:62056412-62056431	None:intergenic	10.0%
!!!	TTTAATTTTGAAAAATGAAT+TGG	+	chr1.3:62057083-62057102	None:intergenic	10.0%
!!	AAAAAACAAGATATTAATCT+AGG	+	chr1.3:62056677-62056696	None:intergenic	15.0%
!!	ATGAAATAAGATCTTAATTT+AGG	+	chr1.3:62056210-62056229	None:intergenic	15.0%
!!	CAACATAAAATATAAATACA+AGG	-	chr1.3:62057218-62057237	MS.gene07250:intron	15.0%
!!	TGAAAAAAATAGATTGTTAT+GGG	+	chr1.3:62057182-62057201	None:intergenic	15.0%
!!	TTGAAAAAAATAGATTGTTA+TGG	+	chr1.3:62057183-62057202	None:intergenic	15.0%
!!!	AAATTTAAGACATTTATCTA+TGG	+	chr1.3:62058168-62058187	None:intergenic	15.0%
!!!	AATGTGTATATGTTATTTTA+TGG	-	chr1.3:62056112-62056131	MS.gene07250:CDS	15.0%
!!!	ATGTGTATATGTTATTTTAT+GGG	-	chr1.3:62056113-62056132	MS.gene07250:CDS	15.0%
!!	AAAACAAATTTAGAAACAGT+CGG	+	chr1.3:62056184-62056203	None:intergenic	20.0%
!!	AAATAGAGTAAAATGTGTAT+AGG	-	chr1.3:62056496-62056515	MS.gene07250:intron	20.0%
!!	AAGAATAAAGGTTTCAAAAT+TGG	+	chr1.3:62057364-62057383	None:intergenic	20.0%
!!	AAGAGTTAATTAAGATATCA+AGG	+	chr1.3:62057990-62058009	None:intergenic	20.0%
!!	TACATATATGAATATATACC+AGG	+	chr1.3:62056064-62056083	None:intergenic	20.0%
!!	TATTCATATATGTATTTGCA+AGG	-	chr1.3:62056070-62056089	MS.gene07250:CDS	20.0%
!!	TTAAATTAAGAGCTTTCAAT+TGG	-	chr1.3:62057799-62057818	MS.gene07250:intron	20.0%
!!	TTTATTAAAAACTAGCAGAA+AGG	+	chr1.3:62057287-62057306	None:intergenic	20.0%
!!!	AAATGTCATTTTTCATTTCA+AGG	-	chr1.3:62058236-62058255	MS.gene07250:CDS	20.0%
!!!	AAGGAAAATTTATTTCTTCA+AGG	-	chr1.3:62059095-62059114	MS.gene07250:intron	20.0%
!!!	AATCGATTTCTCTTATATTA+TGG	-	chr1.3:62057692-62057711	MS.gene07250:intron	20.0%
!!!	AATGTCATTTTTCATTTCAA+GGG	-	chr1.3:62058237-62058256	MS.gene07250:CDS	20.0%
!!!	TATCTTTATTTAGATATAGG+TGG	+	chr1.3:62056409-62056428	None:intergenic	20.0%
!!!	TATGAAAACAAACTATCTTT+TGG	-	chr1.3:62057131-62057150	MS.gene07250:CDS	20.0%
!!!	TCTTAAAACTAAATAGCTTT+TGG	+	chr1.3:62057742-62057761	None:intergenic	20.0%
!!!	TGTATAGTATAGTTAACTTT+TGG	+	chr1.3:62058359-62058378	None:intergenic	20.0%
!	AAAAATGAATTGGATTGCTA+AGG	+	chr1.3:62057073-62057092	None:intergenic	25.0%
!	AAAATGAATTGGATTGCTAA+GGG	+	chr1.3:62057072-62057091	None:intergenic	25.0%
!	AACCTTTATTCTTTCTTCTT+AGG	-	chr1.3:62057371-62057390	MS.gene07250:CDS	25.0%
!	AGATAATGCTAATAAGCATT+GGG	-	chr1.3:62057772-62057791	MS.gene07250:intron	25.0%
!	AGATGACTATGAATATGATT+AGG	-	chr1.3:62056136-62056155	MS.gene07250:CDS	25.0%
!	ATATATGAATATATACCAGG+AGG	+	chr1.3:62056061-62056080	None:intergenic	25.0%
!	ATCCTAAGAAGAAAGAATAA+AGG	+	chr1.3:62057376-62057395	None:intergenic	25.0%
!	GATGACTATGAATATGATTA+GGG	-	chr1.3:62056137-62056156	MS.gene07250:CDS	25.0%
!	TAGATAATGCTAATAAGCAT+TGG	-	chr1.3:62057771-62057790	MS.gene07250:intron	25.0%
!	TGAAATACTTTAGTTATGCT+TGG	-	chr1.3:62058494-62058513	MS.gene07250:intron	25.0%
!	TGACAAACTTCTGAATTAAT+AGG	+	chr1.3:62058460-62058479	None:intergenic	25.0%
!	TGATTCCATATTGTTCATTT+AGG	-	chr1.3:62055984-62056003	MS.gene07250:CDS	25.0%
!	TGTAACCTAAATGAACAATA+TGG	+	chr1.3:62055992-62056011	None:intergenic	25.0%
!	TTAGGATGAAATAGAAAGAA+TGG	-	chr1.3:62057389-62057408	MS.gene07250:CDS	25.0%
!	TTATTAGAAACCTATAATCG+AGG	+	chr1.3:62056703-62056722	None:intergenic	25.0%
!	TTCTTCAAGGAATTGAAATT+AGG	-	chr1.3:62059108-62059127	MS.gene07250:CDS	25.0%
!!	AAGCATCAAATCAGATTTTT+AGG	+	chr1.3:62056451-62056470	None:intergenic	25.0%
!!	ATCACACTCAACATATTTTA+TGG	-	chr1.3:62058061-62058080	MS.gene07250:intron	25.0%
!!	TCACACTCAACATATTTTAT+GGG	-	chr1.3:62058062-62058081	MS.gene07250:intron	25.0%
!!	TGATATGAAGGTTTAGTTTA+AGG	+	chr1.3:62058382-62058401	None:intergenic	25.0%
!!!	CTTCTAAGTTAACTACTTTT+TGG	-	chr1.3:62057030-62057049	MS.gene07250:intron	25.0%
!!!	TAAGTTAACTACTTTTTGGT+TGG	-	chr1.3:62057034-62057053	MS.gene07250:intron	25.0%
	AAAATGATTCCAGACAATGT+CGG	+	chr1.3:62057529-62057548	None:intergenic	30.0%
	AAAGTGAGAGAAGTTCAAAA+TGG	+	chr1.3:62058288-62058307	None:intergenic	30.0%
	AATCAAAACATGTGGATAGA+CGG	-	chr1.3:62057247-62057266	MS.gene07250:intron	30.0%
	ACTTATTACGCAAATTTGAC+AGG	-	chr1.3:62058637-62058656	MS.gene07250:intron	30.0%
	AGAAAATCACTCCAAAATTG+TGG	+	chr1.3:62056633-62056652	None:intergenic	30.0%
	ATTAATTTCGTTGTAGTTGC+TGG	-	chr1.3:62056751-62056770	MS.gene07250:intron	30.0%
	CGATAAAGAGAATTATCAGA+CGG	+	chr1.3:62058419-62058438	None:intergenic	30.0%
	GAAAAAGAGTAGTGAGATAA+AGG	-	chr1.3:62059076-62059095	MS.gene07250:intron	30.0%
	GAAAATCACTCCAAAATTGT+GGG	+	chr1.3:62056632-62056651	None:intergenic	30.0%
	GCTTATCATTGAATTGATTG+TGG	-	chr1.3:62057714-62057733	MS.gene07250:intron	30.0%
	GGAAATACAATCAAAACATG+TGG	-	chr1.3:62057239-62057258	MS.gene07250:intron	30.0%
	GTGACTCATACTATCATAAA+TGG	+	chr1.3:62058586-62058605	None:intergenic	30.0%
	TAAGACATTTATCTATGGCA+TGG	+	chr1.3:62058163-62058182	None:intergenic	30.0%
	TAGAAACTTAAAAGCTAACC+TGG	+	chr1.3:62057933-62057952	None:intergenic	30.0%
	TAGGATCAATCAACAAACAT+AGG	+	chr1.3:62058105-62058124	None:intergenic	30.0%
	TCAAGGGATATAAAGTTGAA+TGG	-	chr1.3:62058253-62058272	MS.gene07250:intron	30.0%
	TCGATTCTCTTAAAATGAGA+AGG	-	chr1.3:62058190-62058209	MS.gene07250:CDS	30.0%
	TTAAAAACTAGCAGAAAGGT+AGG	+	chr1.3:62057283-62057302	None:intergenic	30.0%
	TTCACTTAGAAGGAAAAATG+AGG	+	chr1.3:62058313-62058332	None:intergenic	30.0%
	TTTCAATTGGACAAAAAGAG+TGG	-	chr1.3:62057812-62057831	MS.gene07250:intron	30.0%
!	CATAGCTGAACAATGTTTTT+GGG	+	chr1.3:62056898-62056917	None:intergenic	30.0%
!	TCATAGCTGAACAATGTTTT+TGG	+	chr1.3:62056899-62056918	None:intergenic	30.0%
!	TCTTATTTCATTGTAGTTGC+TGG	-	chr1.3:62056219-62056238	MS.gene07250:CDS	30.0%
!!	ACAAGAAGTATTCACTTAGA+AGG	+	chr1.3:62058323-62058342	None:intergenic	30.0%
!!	AGATTTTTAGGAGATCTGTT+CGG	+	chr1.3:62056439-62056458	None:intergenic	30.0%
!!	TTGCATTTATGTTTGCTGTT+TGG	+	chr1.3:62058912-62058931	None:intergenic	30.0%
!!!	AAAGGAATGTTTTAGAAACG+AGG	+	chr1.3:62057494-62057513	None:intergenic	30.0%
!!!	AAGGAATGTTTTAGAAACGA+GGG	+	chr1.3:62057493-62057512	None:intergenic	30.0%
!!!	GTTTTTTACACCTCGATTAT+AGG	-	chr1.3:62056690-62056709	MS.gene07250:intron	30.0%
	AAAAAAATTCCTTGCTCAGG+GGG	+	chr1.3:62058129-62058148	None:intergenic	35.0%
	AAGCCGCATACTTAGTATTT+GGG	+	chr1.3:62058737-62058756	None:intergenic	35.0%
	AATTGTGCAATGGAACAATG+AGG	+	chr1.3:62056931-62056950	None:intergenic	35.0%
	AGAGCTCTTACAATTGATTG+TGG	-	chr1.3:62057860-62057879	MS.gene07250:intron	35.0%
	AGAGTCGATGAATTGAGAAT+CGG	+	chr1.3:62057559-62057578	None:intergenic	35.0%
	AGTAGATACACTAACAATGC+AGG	+	chr1.3:62058549-62058568	None:intergenic	35.0%
	ATACTTAGTATTTGGGGACA+AGG	+	chr1.3:62058730-62058749	None:intergenic	35.0%
	ATCAAAACATGTGGATAGAC+GGG	-	chr1.3:62057248-62057267	MS.gene07250:intron	35.0%
	CAATGCATGCTACATATTGT+TGG	-	chr1.3:62056952-62056971	MS.gene07250:intron	35.0%
	CACAGAGGTAGTATTCATAA+AGG	+	chr1.3:62058031-62058050	None:intergenic	35.0%
	CATGCATTGTAATTGTGCAA+TGG	+	chr1.3:62056941-62056960	None:intergenic	35.0%
	CGAAAAAAATTCCTTGCTCA+GGG	+	chr1.3:62058131-62058150	None:intergenic	35.0%
	CTAAACCTTCATATCAGTCA+TGG	-	chr1.3:62058386-62058405	MS.gene07250:intron	35.0%
	GAAAAAAATTCCTTGCTCAG+GGG	+	chr1.3:62058130-62058149	None:intergenic	35.0%
	GGATTCATATGCAAAATTCC+AGG	-	chr1.3:62057912-62057931	MS.gene07250:intron	35.0%
	GTTCTGACCATAAATGCATT+TGG	-	chr1.3:62056604-62056623	MS.gene07250:intron	35.0%
	TAAAGAGAATTATCAGACGG+TGG	+	chr1.3:62058416-62058435	None:intergenic	35.0%
	TAAGCCGCATACTTAGTATT+TGG	+	chr1.3:62058738-62058757	None:intergenic	35.0%
	TACTACCTCTGTGAATCAAA+TGG	-	chr1.3:62058038-62058057	MS.gene07250:intron	35.0%
	TATAGACTTCTTCATCTTCG+AGG	+	chr1.3:62059043-62059062	None:intergenic	35.0%
	TATGAATATATACCAGGAGG+CGG	+	chr1.3:62056058-62056077	None:intergenic	35.0%
	TCGAAAAAAATTCCTTGCTC+AGG	+	chr1.3:62058132-62058151	None:intergenic	35.0%
	TGGGAGGGTAAAAATGATTA+TGG	-	chr1.3:62056539-62056558	MS.gene07250:intron	35.0%
	TTTGATGCTTGCACAAGATT+TGG	-	chr1.3:62056461-62056480	MS.gene07250:intron	35.0%
!	AGACTAATTTTGTGCTGCAA+CGG	+	chr1.3:62058836-62058855	None:intergenic	35.0%
!	AGCACCATCCTTTTAACAAA+CGG	-	chr1.3:62056011-62056030	MS.gene07250:CDS	35.0%
!	CAAAGAATACCTTTTCAGCA+TGG	+	chr1.3:62057668-62057687	None:intergenic	35.0%
!	CATTCAACTCGTTTTCAACA+AGG	+	chr1.3:62056096-62056115	None:intergenic	35.0%
!!	AGAAAGAATGGAAGCGTTTA+AGG	-	chr1.3:62057401-62057420	MS.gene07250:CDS	35.0%
!!!	CTGAACAATGTTTTTGGGTT+TGG	+	chr1.3:62056893-62056912	None:intergenic	35.0%
	AACCTTCATATCAGTCATGG+TGG	-	chr1.3:62058389-62058408	MS.gene07250:intron	40.0%
	AGCCGCATACTTAGTATTTG+GGG	+	chr1.3:62058736-62058755	None:intergenic	40.0%
	ATGCCAACAGATAATTCCAC+AGG	+	chr1.3:62058804-62058823	None:intergenic	40.0%
	ATTCCAGACAATGTCGGAAT+TGG	+	chr1.3:62057523-62057542	None:intergenic	40.0%
	CAGGCTCATCAACTAAAATC+CGG	+	chr1.3:62058530-62058549	None:intergenic	40.0%
	GACAAAGCTTAGCAACTTCA+GGG	+	chr1.3:62058665-62058684	None:intergenic	40.0%
	GCTCTTACAATTGATTGTGG+TGG	-	chr1.3:62057863-62057882	MS.gene07250:intron	40.0%
	GTCCCCAAATACTAAGTATG+CGG	-	chr1.3:62058731-62058750	MS.gene07250:CDS	40.0%
	GTGATCCATTTGATTCACAG+AGG	+	chr1.3:62058046-62058065	None:intergenic	40.0%
	GTGGGAACCAAATGCATTTA+TGG	+	chr1.3:62056614-62056633	None:intergenic	40.0%
	TCTTAAAATGAGAAGGCTGC+AGG	-	chr1.3:62058197-62058216	MS.gene07250:CDS	40.0%
	TGAAAGGATCGCCAAATTCT+GGG	-	chr1.3:62056774-62056793	MS.gene07250:intron	40.0%
!	AAATGCAAATGTGCTGCTAC+TGG	-	chr1.3:62058924-62058943	MS.gene07250:intron	40.0%
!	AATGCAAATGTGCTGCTACT+GGG	-	chr1.3:62058925-62058944	MS.gene07250:intron	40.0%
!	AATTAGTCTGCTTGGAACGT+TGG	-	chr1.3:62058847-62058866	MS.gene07250:intron	40.0%
!	CAGCACAAAATTAGTCTGCT+TGG	-	chr1.3:62058839-62058858	MS.gene07250:intron	40.0%
!	GAATTATCTGTTGGCATCGA+AGG	-	chr1.3:62058807-62058826	MS.gene07250:intron	40.0%
!	TGTTGTCGCTTGAAATTCGT+GGG	-	chr1.3:62058688-62058707	MS.gene07250:intron	40.0%
!	TTTAAGATGATTGATGCCCG+TGG	-	chr1.3:62058765-62058784	MS.gene07250:CDS	40.0%
!!	AGAGTGTGCCGTTTGTTAAA+AGG	+	chr1.3:62056022-62056041	None:intergenic	40.0%
!!	AGATAGAGAGCCTTTTTGGT+GGG	+	chr1.3:62057615-62057634	None:intergenic	40.0%
!!	GCATTTGGTTCCCACAATTT+TGG	-	chr1.3:62056619-62056638	MS.gene07250:intron	40.0%
!!	TATGCTTGGTGTACAACTTC+CGG	-	chr1.3:62058508-62058527	MS.gene07250:intron	40.0%
!!	TGTGCCGTTTGTTAAAAGGA+TGG	+	chr1.3:62056018-62056037	None:intergenic	40.0%
!!!	GTTTTTGGGTTTGGAGTCTA+TGG	+	chr1.3:62056884-62056903	None:intergenic	40.0%
	ACGCCTGTGGAATTATCTGT+TGG	-	chr1.3:62058798-62058817	MS.gene07250:intron	45.0%
	ATCCACTCTTTCTCACCCAA+AGG	+	chr1.3:62057896-62057915	None:intergenic	45.0%
	ATCCTCATTGTAGCTGGAGT+TGG	-	chr1.3:62055906-62055925	MS.gene07250:CDS	45.0%
	CATCATCGACCATGCTGAAA+AGG	-	chr1.3:62057656-62057675	MS.gene07250:intron	45.0%
	CGACAAAGCTTAGCAACTTC+AGG	+	chr1.3:62058666-62058685	None:intergenic	45.0%
	CGATGAATTGAGAATCGGCA+GGG	+	chr1.3:62057554-62057573	None:intergenic	45.0%
	CGTACTACTCCCATTGATGT+CGG	-	chr1.3:62057459-62057478	MS.gene07250:CDS	45.0%
	CGTTTAAGGAATTGCCGGAA+GGG	-	chr1.3:62057415-62057434	MS.gene07250:CDS	45.0%
	GAAAGAATCACCGAAGTACG+AGG	-	chr1.3:62056243-62056262	MS.gene07250:CDS	45.0%
	GAAAGGATCGCCAAATTCTG+GGG	-	chr1.3:62056775-62056794	MS.gene07250:intron	45.0%
	GACCACCATGACTGATATGA+AGG	+	chr1.3:62058394-62058413	None:intergenic	45.0%
	GATTGATGCCCGTGGATTTA+AGG	-	chr1.3:62058773-62058792	MS.gene07250:intron	45.0%
	GGATCTCGGAAATCCCTATT+CGG	+	chr1.3:62055890-62055909	None:intergenic	45.0%
	GGGTGGTTGGAGATTAAGAT+GGG	-	chr1.3:62058999-62059018	MS.gene07250:CDS	45.0%
	GTCCTAGACTGAGAATCGAT+GGG	-	chr1.3:62058979-62058998	MS.gene07250:CDS	45.0%
	GTGAAAGGATCGCCAAATTC+TGG	-	chr1.3:62056773-62056792	MS.gene07250:intron	45.0%
	TACCAACTCCAGCTACAATG+AGG	+	chr1.3:62055911-62055930	None:intergenic	45.0%
	TATTTCCCCCTCTCTCTCAA+TGG	-	chr1.3:62055929-62055948	MS.gene07250:CDS	45.0%
	TCACGCAACAGAGCAAGATT+GGG	-	chr1.3:62058954-62058973	MS.gene07250:intron	45.0%
	TCAGCTTGATCCCCAGAATT+TGG	+	chr1.3:62056788-62056807	None:intergenic	45.0%
	TCCAGCTACAATGAGGATCT+CGG	+	chr1.3:62055904-62055923	None:intergenic	45.0%
	TCGATGAATTGAGAATCGGC+AGG	+	chr1.3:62057555-62057574	None:intergenic	45.0%
	TCGTTGTAGTTGCTGGTGAA+AGG	-	chr1.3:62056758-62056777	MS.gene07250:intron	45.0%
	TGGGTGGTTGGAGATTAAGA+TGG	-	chr1.3:62058998-62059017	MS.gene07250:CDS	45.0%
	TTGGCTTCAACGAACAAACG+TGG	+	chr1.3:62058893-62058912	None:intergenic	45.0%
!	AAAGTGCCATTGAGAGAGAG+GGG	+	chr1.3:62055938-62055957	None:intergenic	45.0%
!	AATTCTGGGGATCAAGCTGA+AGG	-	chr1.3:62056788-62056807	MS.gene07250:intron	45.0%
!	AGCGAGATAGAGAGCCTTTT+TGG	+	chr1.3:62057619-62057638	None:intergenic	45.0%
!	AGTAGTACGAGAGAGTATGG+CGG	+	chr1.3:62057448-62057467	None:intergenic	45.0%
!	AGTCTATGGCTTTTCGTGAC+GGG	+	chr1.3:62056870-62056889	None:intergenic	45.0%
!	GAGATAGAGAGCCTTTTTGG+TGG	+	chr1.3:62057616-62057635	None:intergenic	45.0%
!	GAGTCTATGGCTTTTCGTGA+CGG	+	chr1.3:62056871-62056890	None:intergenic	45.0%
!	GTGTTGTCGCTTGAAATTCG+TGG	-	chr1.3:62058687-62058706	MS.gene07250:intron	45.0%
!	TGGTTTGGATTATGCAGGTC+GGG	-	chr1.3:62056319-62056338	MS.gene07250:CDS	45.0%
!	TTATCTGTTGGCATCGAAGG+TGG	-	chr1.3:62058810-62058829	MS.gene07250:intron	45.0%
!!	CGAACTGGTTTGGATTATGC+AGG	-	chr1.3:62056314-62056333	MS.gene07250:CDS	45.0%
!!	CTGGTTTGGATTATGCAGGT+CGG	-	chr1.3:62056318-62056337	MS.gene07250:CDS	45.0%
!!	GAAAGTGCCATTGAGAGAGA+GGG	+	chr1.3:62055939-62055958	None:intergenic	45.0%
!!	GGAAGCGTTTAAGGAATTGC+CGG	-	chr1.3:62057410-62057429	MS.gene07250:CDS	45.0%
!!	TGAAAGTGCCATTGAGAGAG+AGG	+	chr1.3:62055940-62055959	None:intergenic	45.0%
!!	TGTTGATTCAGCTGATGCTC+AGG	-	chr1.3:62056817-62056836	MS.gene07250:intron	45.0%
	AAGTACGAGGATGGAGCTGA+TGG	-	chr1.3:62056256-62056275	MS.gene07250:CDS	50.0%
	AATTCCTTGCTCAGGGGGAT+AGG	+	chr1.3:62058124-62058143	None:intergenic	50.0%
	ACTGAGAATCGATGGGTGGT+TGG	-	chr1.3:62058986-62059005	MS.gene07250:CDS	50.0%
	AGGCGTGTCCTTAAATCCAC+GGG	+	chr1.3:62058784-62058803	None:intergenic	50.0%
	CACTCTTTCTCACCCAAAGG+AGG	+	chr1.3:62057893-62057912	None:intergenic	50.0%
	CAGGCGTGTCCTTAAATCCA+CGG	+	chr1.3:62058785-62058804	None:intergenic	50.0%
	CCAGACAATGTCGGAATTGG+CGG	+	chr1.3:62057520-62057539	None:intergenic	50.0%
	CCAGTTCGTTACCTGAGCAT+CGG	+	chr1.3:62056302-62056321	None:intergenic	50.0%
	CGTCCTAGACTGAGAATCGA+TGG	-	chr1.3:62058978-62058997	MS.gene07250:CDS	50.0%
	CTAGACTGAGAATCGATGGG+TGG	-	chr1.3:62058982-62059001	MS.gene07250:CDS	50.0%
	CTCCTTTGGGTGAGAAAGAG+TGG	-	chr1.3:62057891-62057910	MS.gene07250:intron	50.0%
	GAATATATACCAGGAGGCGG+CGG	+	chr1.3:62056055-62056074	None:intergenic	50.0%
	GAATCACCGAAGTACGAGGA+TGG	-	chr1.3:62056247-62056266	MS.gene07250:CDS	50.0%
	GCGTTTAAGGAATTGCCGGA+AGG	-	chr1.3:62057414-62057433	MS.gene07250:CDS	50.0%
	GCTCAGGTAACGAACTGGTT+TGG	-	chr1.3:62056304-62056323	MS.gene07250:CDS	50.0%
	GGGAGTAGTACGAGAGAGTA+TGG	+	chr1.3:62057451-62057470	None:intergenic	50.0%
	GTCACGCAACAGAGCAAGAT+TGG	-	chr1.3:62058953-62058972	MS.gene07250:intron	50.0%
	TCAGCTCCATCCTCGTACTT+CGG	+	chr1.3:62056256-62056275	None:intergenic	50.0%
	TCCGAGATCCTCATTGTAGC+TGG	-	chr1.3:62055900-62055919	MS.gene07250:CDS	50.0%
	TGCAACTGCTCCCACCAAAA+AGG	-	chr1.3:62057602-62057621	MS.gene07250:CDS	50.0%
	TGGATTTAAGGACACGCCTG+TGG	-	chr1.3:62058785-62058804	MS.gene07250:intron	50.0%
	TGTGGTGGTATGCCTCCTTT+GGG	-	chr1.3:62057878-62057897	MS.gene07250:intron	50.0%
	TTGTGGTGGTATGCCTCCTT+TGG	-	chr1.3:62057877-62057896	MS.gene07250:intron	50.0%
	TTTGGTGGGAGCAGTTGCAA+GGG	+	chr1.3:62057601-62057620	None:intergenic	50.0%
!	AAGTGCCATTGAGAGAGAGG+GGG	+	chr1.3:62055937-62055956	None:intergenic	50.0%
!	GATGGAGCTGATGGTGAAGA+TGG	-	chr1.3:62056265-62056284	MS.gene07250:CDS	50.0%
!	TTTTGGTGGGAGCAGTTGCA+AGG	+	chr1.3:62057602-62057621	None:intergenic	50.0%
!!	CACCCATCGATTCTCAGTCT+AGG	+	chr1.3:62058984-62059003	None:intergenic	50.0%
!!	TGTTGATTCCACCGATGCTC+AGG	-	chr1.3:62056288-62056307	MS.gene07250:CDS	50.0%
	CCGATGCTCAGGTAACGAAC+TGG	-	chr1.3:62056299-62056318	MS.gene07250:CDS	55.0%
	CCGCCAATTCCGACATTGTC+TGG	-	chr1.3:62057517-62057536	MS.gene07250:CDS	55.0%
	CTACTCCCATTGATGTCGGC+AGG	-	chr1.3:62057463-62057482	MS.gene07250:CDS	55.0%
	GAGAGCCTGCCGACATCAAT+GGG	+	chr1.3:62057471-62057490	None:intergenic	55.0%
	GAGCGAGTGATCATGTGCGT+GGG	-	chr1.3:62056520-62056539	MS.gene07250:intron	55.0%
	GAGTGATCATGTGCGTGGGA+GGG	-	chr1.3:62056524-62056543	MS.gene07250:intron	55.0%
	TGATCCTATCCCCCTGAGCA+AGG	-	chr1.3:62058117-62058136	MS.gene07250:intron	55.0%
	TGATTTATTCCGCCGCCTCC+TGG	-	chr1.3:62056043-62056062	MS.gene07250:CDS	55.0%
	TGTCGGAATTGGCGGCAGAA+AGG	+	chr1.3:62057512-62057531	None:intergenic	55.0%
!	AATGTGCTGCTACTGGGTGG+AGG	-	chr1.3:62058931-62058950	MS.gene07250:intron	55.0%
!	GCAAATGTGCTGCTACTGGG+TGG	-	chr1.3:62058928-62058947	MS.gene07250:intron	55.0%
!!	GTTCGTTACCTGAGCATCGG+TGG	+	chr1.3:62056299-62056318	None:intergenic	55.0%
	ATGGCGGCAATGCACCCTTC+CGG	+	chr1.3:62057432-62057451	None:intergenic	60.0%
	CGAGCGAGTGATCATGTGCG+TGG	-	chr1.3:62056519-62056538	MS.gene07250:intron	60.0%
	CGAGTGATCATGTGCGTGGG+AGG	-	chr1.3:62056523-62056542	MS.gene07250:intron	60.0%
	GGAGAGCCTGCCGACATCAA+TGG	+	chr1.3:62057472-62057491	None:intergenic	60.0%
	GTGGGAGCAGTTGCAAGGGA+AGG	+	chr1.3:62057597-62057616	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	62055876	62059148	62055876	ID=MS.gene07250
chr1.3	mRNA	62055876	62059148	62055876	ID=MS.gene07250.t1;Parent=MS.gene07250
chr1.3	exon	62059097	62059148	62059097	ID=MS.gene07250.t1.exon1;Parent=MS.gene07250.t1
chr1.3	CDS	62059097	62059148	62059097	ID=cds.MS.gene07250.t1;Parent=MS.gene07250.t1
chr1.3	exon	62058960	62059018	62058960	ID=MS.gene07250.t1.exon2;Parent=MS.gene07250.t1
chr1.3	CDS	62058960	62059018	62058960	ID=cds.MS.gene07250.t1;Parent=MS.gene07250.t1
chr1.3	exon	62058715	62058789	62058715	ID=MS.gene07250.t1.exon3;Parent=MS.gene07250.t1
chr1.3	CDS	62058715	62058789	62058715	ID=cds.MS.gene07250.t1;Parent=MS.gene07250.t1
chr1.3	exon	62058186	62058257	62058186	ID=MS.gene07250.t1.exon4;Parent=MS.gene07250.t1
chr1.3	CDS	62058186	62058257	62058186	ID=cds.MS.gene07250.t1;Parent=MS.gene07250.t1
chr1.3	exon	62057347	62057631	62057347	ID=MS.gene07250.t1.exon5;Parent=MS.gene07250.t1
chr1.3	CDS	62057347	62057631	62057347	ID=cds.MS.gene07250.t1;Parent=MS.gene07250.t1
chr1.3	exon	62057091	62057215	62057091	ID=MS.gene07250.t1.exon6;Parent=MS.gene07250.t1
chr1.3	CDS	62057091	62057215	62057091	ID=cds.MS.gene07250.t1;Parent=MS.gene07250.t1
chr1.3	exon	62055876	62056365	62055876	ID=MS.gene07250.t1.exon7;Parent=MS.gene07250.t1
chr1.3	CDS	62055876	62056365	62055876	ID=cds.MS.gene07250.t1;Parent=MS.gene07250.t1

Gene Sequence

>MS.gene07250

ATGAGCACGAGCCGAATAGGGATTTCCGAGATCCTCATTGTAGCTGGAGTTGGTATTTCCCCCTCTCTCTCAATGGCACTTTCATCAACATTTTTTTCTGTGCTCGTCTGATTCCATATTGTTCATTTAGGTTACAGCACCATCCTTTTAACAAACGGCACACTCTCTGATTTATTCCGCCGCCTCCTGGTATATATTCATATATGTATTTGCAAGGCCTTGTTGAAAACGAGTTGAATGTGTATATGTTATTTTATGGGAGATGACTATGAATATGATTAGGGTTTCATTGTCTCAAACTAAAGCCGACTGTTTCTAAATTTGTTTTCTTCCTAAATTAAGATCTTATTTCATTGTAGTTGCTGGTGAAAGAATCACCGAAGTACGAGGATGGAGCTGATGGTGAAGATGGTGTTGATTCCACCGATGCTCAGGTAACGAACTGGTTTGGATTATGCAGGTCGGGCAAAATATGTTTTGAATATCGAACACTGTAAGATACACAGTTGAATTTATCACTTGATTTGATGCCACCTATATCTAAATAAAGATAAATGCAACCGAACAGATCTCCTAAAAATCTGATTTGATGCTTGCACAAGATTTGGTATTCTATATTGAAATAGAGTAAAATGTGTATAGGCGAGCGAGTGATCATGTGCGTGGGAGGGTAAAAATGATTATGGTTTCAGAATATTCTTGTTTAATTTTTCATCTAATTGTCGATAGTTCTGACCATAAATGCATTTGGTTCCCACAATTTTGGAGTGATTTTCTCAAACTAAACTGTGTTTTCTTCCTAGATTAATATCTTGTTTTTTACACCTCGATTATAGGTTTCTAATAAACTCTGATTTTGTTAAATTCTGTATTAAATTAATTTCGTTGTAGTTGCTGGTGAAAGGATCGCCAAATTCTGGGGATCAAGCTGAAGGTGAAGATGTTGATTCAGCTGATGCTCAGGTAACAATCTACGTTGATTATGTCTCAACCCGTCACGAAAAGCCATAGACTCCAAACCCAAAAACATTGTTCAGCTATGAGTTTCTTCTCCTCATTGTTCCATTGCACAATTACAATGCATGCTACATATTGTTGGATTTTTCACTCGATTTGATGCTTGCACATGTATATATACTATTATATAAAAGCAACTTCTAAGTTAACTACTTTTTGGTTGGACTTTTTAACTACCCTTAGCAATCCAATTCATTTTTCAAAATTAAATTTAAATCTTTGTCTAAGTTATAGAAAATATGAAAACAAACTATCTTTTGGTTGAATAACTTCTTCTACAAATTTTCCCATAACAATCTATTTTTTTCAAATTTAAAGTTAAAAACAACATAAAATATAAATACAAGGAAATACAATCAAAACATGTGGATAGACGGGCACGTAGTGCCTACCTTTCTGCTAGTTTTTAATAAAATCATAAACACTACTTAACACTAAAAAACTAGTATAAATACAGCAATAACTAGACCAATTTTGAAACCTTTATTCTTTCTTCTTAGGATGAAATAGAAAGAATGGAAGCGTTTAAGGAATTGCCGGAAGGGTGCATTGCCGCCATACTCTCTCGTACTACTCCCATTGATGTCGGCAGGCTCTCCCTCGTTTCTAAAACATTCCTTTCTGCCGCCAATTCCGACATTGTCTGGAATCATTTTCTCCCTGCCGATTCTCAATTCATCGACTCTATCATCTCTCAGTATCCTTCCCTTGCAACTGCTCCCACCAAAAAGGCTCTCTATCTCGCTCTATCTGATCATACAATCATCATCGACCATGCTGAAAAGGTATTCTTTGTCACAATCGATTTCTCTTATATTATGGCTTATCATTGAATTGATTGTGGTGCCAAAAGCTATTTAGTTTTAAGATGTTTAGTTTAGATAATGCTAATAAGCATTGGGTTGTTTAAATTAAGAGCTTTCAATTGGACAAAAAGAGTGGAAAGAAGTGTTACATGCTTGCTGCTAGAGCTCTTACAATTGATTGTGGTGGTATGCCTCCTTTGGGTGAGAAAGAGTGGATTCATATGCAAAATTCCAGGTTAGCTTTTAAGTTTCTAAGTTTTTTTCTTAAATGTTAATTATACTATTATTCCTTGATATCTTAATTAACTCTTAATCAACAGTTATCTAAACCTTTATGAATACTACCTCTGTGAATCAAATGGATCACACTCAACATATTTTATGGGCTTGAATGATTCTATTTCCTATGTTTGTTGATTGATCCTATCCCCCTGAGCAAGGAATTTTTTTCGACTTTTAGACCATGCCATAGATAAATGTCTTAAATTTCATCGATTCTCTTAAAATGAGAAGGCTGCAGGTTGTATGAAATAACTGAAATGTCATTTTTCATTTCAAGGGATATAAAGTTGAATGGAAATATTTTCCATTTTGAACTTCTCTCACTTTCTCCTCATTTTTCCTTCTAAGTGAATACTTCTTGTTTAACTATTAAATCCAAAAGTTAACTATACTATACACCTTAAACTAAACCTTCATATCAGTCATGGTGGTCCACCGTCTGATAATTCTCTTTATCGATAACAAACAATATATAACCTATTAATTCAGAAGTTTGTCATAATCATTTACTATTGAAATACTTTAGTTATGCTTGGTGTACAACTTCCGGATTTTAGTTGATGAGCCTGCATTGTTAGTGTATCTACTCGAGTATAAAAGCTCCATTTATGATAGTATGAGTCACTTTCGACTTCTTTTTTTTTTTTATCTTTGTAACTTATTACGCAAATTTGACAGGTTCCCTGAAGTTGCTAAGCTTTGTCGTGTGTTGTCGCTTGAAATTCGTGGGATGATTAACACTCTTTCCTTGTCCCCAAATACTAAGTATGCGGCTTATCTCGTGTTTAAGATGATTGATGCCCGTGGATTTAAGGACACGCCTGTGGAATTATCTGTTGGCATCGAAGGTGGCCGTTGCAGCACAAAATTAGTCTGCTTGGAACGTTGGCTACACAACAAAGTAACACACCACGTTTGTTCGTTGAAGCCAAACAGCAAACATAAATGCAAATGTGCTGCTACTGGGTGGAGGTCACGCAACAGAGCAAGATTGGGACGTCCTAGACTGAGAATCGATGGGTGGTTGGAGATTAAGATGGGTGAATTCTTCAATTCAAGCCTCGAAGATGAAGAAGTCTATATGAGTGTTATCGAGAAAAAGAGTAGTGAGATAAAGGAAAATTTATTTCTTCAAGGAATTGAAATTAGGCCTGCCAAAGACAATTGA

Protein sequence

>MS.gene07250.t1

MSTSRIGISEILIVAGVGYSTILLTNGTLSDLFRRLLLLVKESPKYEDGADGEDGVDSTDAQLLVKGSPNSGDQAEGEDVDSADAQDEIERMEAFKELPEGCIAAILSRTTPIDVGRLSLVSKTFLSAANSDIVWNHFLPADSQFIDSIISQYPSLATAPTKKALYLALSDHTIIIDHAEKSFQLDKKSGKKCYMLAARALTIDCGGMPPLGEKEWIHMQNSRFPEVAKLCRVLSLEIRGMINTLSLSPNTKYAAYLVFKMIDARGFKDTPVELSVGIEGGRCSTKLVCLERWLHNKVTHHVCSLKPNSKHKCKCAATGWRSRNRARLGRPRLRIDGWLEIKMGEFFNSSLEDEEVYMSVIEKKSSEIKENLFLQGIEIRPAKDN