AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52533

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52533.t1 MTR_1g079180 77.736 265 53 3 1 259 1 265 3.61e-144 404
MS.gene52533.t1 MTR_1g079370 70.566 265 72 4 1 259 1 265 5.49e-128 363
MS.gene52533.t1 MTR_1g079230 68.864 273 71 4 1 259 1 273 2.19e-125 357
MS.gene52533.t1 MTR_1g079200 72.075 265 67 5 1 259 1 264 1.99e-124 354
MS.gene52533.t1 MTR_1g079380 72.453 265 67 4 1 259 1 265 9.02e-123 352
MS.gene52533.t1 MTR_1g079460 68.797 266 76 4 1 259 1 266 7.92e-122 348
MS.gene52533.t1 MTR_1g079260 65.934 273 78 6 1 259 1 272 3.15e-119 342
MS.gene52533.t1 MTR_1g100597 66.667 273 76 5 1 259 1 272 7.89e-119 341
MS.gene52533.t1 MTR_1g079850 62.989 281 82 5 1 259 1 281 1.28e-107 313
MS.gene52533.t1 MTR_1g100613 66.390 241 66 3 20 245 5 245 5.31e-104 302
MS.gene52533.t1 MTR_1g079250 60.072 278 92 7 1 259 1 278 1.24e-101 298
MS.gene52533.t1 MTR_1g026510 57.244 283 97 7 1 259 1 283 6.60e-100 294
MS.gene52533.t1 MTR_3g452600 61.154 260 95 5 4 259 10 267 1.07e-96 284
MS.gene52533.t1 MTR_3g006850 54.849 299 92 5 4 259 17 315 1.31e-91 273
MS.gene52533.t1 MTR_3g450990 56.522 276 98 7 4 259 10 283 3.36e-91 271
MS.gene52533.t1 MTR_1g079240 73.370 184 39 3 80 259 2 179 7.70e-90 264
MS.gene52533.t1 MTR_1g079350 56.786 280 95 11 1 259 1 275 2.66e-87 261
MS.gene52533.t1 MTR_1g079340 53.310 287 105 8 1 259 40 325 2.65e-86 260
MS.gene52533.t1 MTR_1g079410 53.731 268 114 6 2 259 6 273 1.08e-85 256
MS.gene52533.t1 MTR_7g096300 59.677 248 83 6 1 239 1 240 1.45e-80 257
MS.gene52533.t1 MTR_2g079100 54.962 262 102 6 3 256 8 261 6.45e-79 239
MS.gene52533.t1 MTR_1g078470 71.006 169 46 3 93 259 17 184 3.84e-77 231
MS.gene52533.t1 MTR_1g079140 44.781 297 120 8 4 259 13 306 8.36e-68 212
MS.gene52533.t1 MTR_1g079400 44.523 283 113 7 5 259 13 279 2.57e-64 202
MS.gene52533.t1 MTR_7g112550 43.929 280 134 9 2 259 5 283 1.31e-61 196
MS.gene52533.t1 MTR_1g083580 38.406 276 152 7 2 259 6 281 4.41e-56 181
MS.gene52533.t1 MTR_7g112560 41.199 267 136 10 4 259 1 257 5.08e-48 160
MS.gene52533.t1 MTR_1g078460 86.207 87 12 0 1 87 64 150 7.02e-47 153
MS.gene52533.t1 MTR_1g079350 65.546 119 35 2 1 116 1 116 5.74e-44 148
MS.gene52533.t1 MTR_4g023570 39.924 263 125 10 4 259 1 237 1.91e-43 147
MS.gene52533.t1 MTR_1g076870 59.091 132 35 2 127 239 2 133 2.20e-41 139
MS.gene52533.t1 MTR_2g042540 61.667 120 43 3 1 118 1 119 6.63e-38 130
MS.gene52533.t1 MTR_1g079210 55.833 120 39 1 129 234 8 127 4.65e-35 122
MS.gene52533.t1 MTR_7g096310 57.480 127 47 3 57 181 38 159 2.76e-34 122
MS.gene52533.t1 MTR_3g058640 57.000 100 43 0 127 226 7 106 6.09e-31 111
MS.gene52533.t1 MTR_1g079220 77.273 66 15 0 164 229 1 66 1.72e-30 108
MS.gene52533.t1 MTR_1g079130 40.000 190 82 6 101 259 1 189 2.84e-28 107
MS.gene52533.t1 MTR_7g103380 25.455 275 177 7 3 258 18 283 3.35e-24 99.0
MS.gene52533.t1 MTR_0002s0020 34.028 144 86 3 6 141 24 166 7.55e-21 89.7
MS.gene52533.t1 MTR_0113s0040 32.086 187 114 7 2 180 20 201 3.25e-20 87.8
MS.gene52533.t1 MTR_5g010800 34.483 145 87 5 2 141 15 156 2.84e-19 85.1
MS.gene52533.t1 MTR_4g102770 35.000 140 86 3 6 141 21 159 1.47e-18 83.2
MS.gene52533.t1 MTR_1g101390 34.722 144 88 4 2 141 26 167 4.55e-18 82.0
MS.gene52533.t1 MTR_4g083970 28.342 187 109 6 80 259 122 290 5.31e-14 70.5
MS.gene52533.t1 MTR_1g079390 67.347 49 11 2 209 254 9 55 2.31e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52533.t1 AT1G80110 45.736 258 128 8 7 258 4 255 2.00e-63 199
MS.gene52533.t1 AT2G02360 41.667 264 140 6 2 258 12 268 1.38e-61 195
MS.gene52533.t1 AT2G02250 40.613 261 145 7 4 258 47 303 2.26e-56 183
MS.gene52533.t1 AT2G02240 39.768 259 149 4 4 258 61 316 7.42e-55 179
MS.gene52533.t1 AT2G02340 38.889 270 152 7 1 258 33 301 1.48e-52 173
MS.gene52533.t1 AT2G02230 35.161 310 143 8 2 259 30 333 1.77e-52 174
MS.gene52533.t1 AT1G09155 41.219 279 137 10 7 259 3 280 2.82e-50 167
MS.gene52533.t1 AT2G02350 36.466 266 155 8 2 258 22 282 6.36e-48 160
MS.gene52533.t1 AT1G56240 38.849 278 143 10 7 258 4 280 6.58e-46 155
MS.gene52533.t1 AT2G02320 37.687 268 147 9 3 258 39 298 7.06e-45 153
MS.gene52533.t1 AT1G56250 39.785 279 141 10 7 259 4 281 1.30e-44 152
MS.gene52533.t1 AT2G02310 33.962 265 165 5 2 259 43 304 1.11e-42 147
MS.gene52533.t1 AT2G02300 37.407 270 137 9 2 259 32 281 4.71e-40 140
MS.gene52533.t1 AT5G24560 46.429 140 71 2 3 141 2 138 5.54e-34 122
MS.gene52533.t1 AT3G53000 27.985 268 172 9 6 258 23 284 4.64e-26 103
MS.gene52533.t1 AT2G02350 34.731 167 98 7 101 258 1 165 5.36e-22 90.1
MS.gene52533.t1 AT3G61060 29.012 162 109 4 6 164 27 185 4.86e-19 84.7
MS.gene52533.t1 AT1G63090 33.540 161 102 4 6 164 28 185 9.04e-19 84.0
MS.gene52533.t1 AT1G12710 35.714 140 83 4 6 141 30 166 3.39e-18 82.4
MS.gene52533.t1 AT3G61060 27.607 163 111 4 6 164 27 186 1.49e-17 80.5
MS.gene52533.t1 AT5G52120 28.125 192 123 7 1 180 18 206 3.45e-14 71.2
MS.gene52533.t1 AT1G33920 30.000 170 92 5 100 259 12 164 3.25e-13 66.2
MS.gene52533.t1 AT4G19850 29.050 179 95 6 94 259 17 176 4.26e-13 66.2
MS.gene52533.t1 AT4G19850 29.050 179 95 6 94 259 59 218 4.82e-13 67.0
MS.gene52533.t1 AT1G65390 30.000 180 95 7 91 259 251 410 9.32e-13 67.8
MS.gene52533.t1 AT1G12710 35.714 126 74 4 6 127 30 152 1.20e-12 67.0
MS.gene52533.t1 AT4G19840 29.167 168 96 6 99 259 94 245 2.44e-12 65.5

Find 66 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCTGAAATTCATCTTCT+AGG 0.185837 1.3:+56264036 None:intergenic
GGATTGTTGTGCCTGATTCT+AGG 0.284145 1.3:-56265162 MS.gene52533:intron
TAGGCCGCATACTGAGTATT+TGG 0.307750 1.3:+56264272 None:intergenic
TGCGGCCTACCTTCCACATT+TGG 0.313256 1.3:+56264143 None:intergenic
ATCGGAGCGTTCTTCACTTC+AGG 0.343110 1.3:-56264059 MS.gene52533:CDS
TGCCAACGTTCCAACCAAAA+AGG 0.356121 1.3:-56265744 MS.gene52533:CDS
TAGATCTCTCTCCATTGCTT+GGG 0.357129 1.3:-56265206 MS.gene52533:CDS
TGAATTTCAGATGAATGTTA+AGG 0.372056 1.3:-56264027 MS.gene52533:CDS
TGACCATGATCGATGATGAT+AGG 0.382423 1.3:+56265695 None:intergenic
CTAGATCTCTCTCCATTGCT+TGG 0.387570 1.3:-56265207 MS.gene52533:CDS
ATGTTAAGGGGACAAATAAT+TGG 0.389612 1.3:-56264013 MS.gene52533:CDS
CCAGCTTGCTGGTCTTACTT+AGG 0.397146 1.3:+56263954 None:intergenic
GAGAATCTGCCAACGTCAAT+AGG 0.399491 1.3:+56265869 None:intergenic
TGAAATGCAGAGCTTTCAAT+TGG 0.399907 1.3:-56265266 MS.gene52533:intron
TGTGGTGGCTTGGTATTTGT+TGG 0.406536 1.3:-56264319 MS.gene52533:CDS
TAGTACTAAAGTTGTCTGTT+TGG 0.412140 1.3:-56264168 MS.gene52533:CDS
CTTCATCATGTGTGGTGGCT+TGG 0.417860 1.3:-56264329 MS.gene52533:CDS
CCGCCGATTCTGATGATGTC+TGG 0.418266 1.3:-56265820 MS.gene52533:CDS
TTAAGAGTTAACCTAGAATC+AGG 0.435504 1.3:+56265151 None:intergenic
ATGATGGCAATACACTCTTC+AGG 0.438660 1.3:+56265908 None:intergenic
AATCATCTTGACCACAAGAT+AGG 0.444114 1.3:+56264253 None:intergenic
AAGAGAGGTCTCTTGCTTGA+AGG 0.444562 1.3:-56263990 MS.gene52533:CDS
TGCTTGAAGGAATAGAAGTT+AGG 0.447787 1.3:-56263977 MS.gene52533:CDS
GGATAGATGATATGTCAGAA+GGG 0.448503 1.3:+56265786 None:intergenic
GAATTTCAGATGAATGTTAA+GGG 0.453633 1.3:-56264026 MS.gene52533:CDS
AGGATAGATGATATGTCAGA+AGG 0.463882 1.3:+56265785 None:intergenic
CGTCCTAGTGTAAGAAGTGA+TGG 0.475634 1.3:-56264098 MS.gene52533:CDS
GAGGACAGTTCTGAAATCCT+TGG 0.485119 1.3:+56264225 None:intergenic
CATCATCGATCATGGTCAAA+AGG 0.487205 1.3:-56265690 MS.gene52533:intron
GTGATGATCGATATTGCAAC+TGG 0.493993 1.3:-56265183 MS.gene52533:CDS
AGGATTTCAGAACTGTCCTC+TGG 0.500268 1.3:-56264222 MS.gene52533:CDS
AGGCCGCAAAACAAAGTAGT+AGG 0.501061 1.3:-56264128 MS.gene52533:CDS
CGTCCTATCATCATCGATCA+TGG 0.502262 1.3:-56265698 MS.gene52533:CDS
AGGACAGTTCTGAAATCCTT+GGG 0.509833 1.3:+56264226 None:intergenic
ATTCCAGACATCATCAGAAT+CGG 0.524392 1.3:+56265817 None:intergenic
GTTGGAACGTTGGCAAGTGA+AGG 0.538767 1.3:+56265752 None:intergenic
TGTAAGAAGTGATGGGTGGT+TGG 0.556049 1.3:-56264090 MS.gene52533:CDS
TCGAAAAGTCTTAGAAAAGA+CGG 0.565928 1.3:+56265847 None:intergenic
TTTCAATTGGATAGAAAGAG+TGG 0.567809 1.3:-56265253 MS.gene52533:CDS
CACCCATCACTTCTTACACT+AGG 0.568450 1.3:+56264095 None:intergenic
TCGATCATCACCCCAAGCAA+TGG 0.574917 1.3:+56265195 None:intergenic
GGGACAAATAATTGGAAGAG+AGG 0.583609 1.3:-56264005 MS.gene52533:CDS
AGATCTCTCTCCATTGCTTG+GGG 0.585076 1.3:-56265205 MS.gene52533:CDS
TGGATCCAAATGTGGAAGGT+AGG 0.591841 1.3:-56264148 MS.gene52533:CDS
AATTTCAGATGAATGTTAAG+GGG 0.593843 1.3:-56264025 MS.gene52533:CDS
CTGGAGTTAACAGTTGGTGT+CGG 0.617888 1.3:-56264203 MS.gene52533:CDS
GTCTCCAAATACTCAGTATG+CGG 0.620795 1.3:-56264276 MS.gene52533:CDS
TGTCCTCTGGAGTTAACAGT+TGG 0.628874 1.3:-56264209 MS.gene52533:CDS
AGACAACTTTAGTACTACAA+TGG 0.631885 1.3:+56264174 None:intergenic
GTCCTAGTGTAAGAAGTGAT+GGG 0.632320 1.3:-56264097 MS.gene52533:CDS
TGTCTGTTTGGATCCAAATG+TGG 0.635689 1.3:-56264156 MS.gene52533:CDS
GGGTGGTTGGAGATTGAGAT+CGG 0.638054 1.3:-56264077 MS.gene52533:CDS
GAGTTAACAGTTGGTGTCGG+AGG 0.640097 1.3:-56264200 MS.gene52533:CDS
GAGTATTTGGAGACAAGGCA+AGG 0.643849 1.3:+56264285 None:intergenic
CCAGACATCATCAGAATCGG+CGG 0.648846 1.3:+56265820 None:intergenic
ATACTGAGTATTTGGAGACA+AGG 0.649595 1.3:+56264280 None:intergenic
CAAGTGAAGGAGACTGTGAG+AGG 0.655481 1.3:+56265765 None:intergenic
CGCACGACTCCTATTGACGT+TGG 0.658635 1.3:-56265878 MS.gene52533:CDS
ACACCAACTGTTAACTCCAG+AGG 0.659248 1.3:+56264206 None:intergenic
AGTCGTGCGAGAGAGTATGA+TGG 0.661233 1.3:+56265892 None:intergenic
TCAGTATGCGGCCTATCTTG+TGG 0.662251 1.3:-56264264 MS.gene52533:CDS
GTCAAGATGATTGATGCCCA+AGG 0.677582 1.3:-56264242 MS.gene52533:CDS
GATGAAGAAAAGCAACCTCG+TGG 0.684416 1.3:+56264345 None:intergenic
TGTGTTGGACAGGTTCCACG+AGG 0.693072 1.3:-56264360 MS.gene52533:intron
TGTTTGGATCCAAATGTGGA+AGG 0.703248 1.3:-56264152 MS.gene52533:CDS
CTAGTGTAAGAAGTGATGGG+TGG 0.744077 1.3:-56264094 MS.gene52533:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTATTGAAGTTTAAATTT+GGG + chr1.3:56265311-56265330 None:intergenic 15.0%
!! TGAAATAATTTAGTTATCAT+TGG - chr1.3:56265418-56265437 MS.gene52533:intron 15.0%
!!! GCTTATGTTTTTTAAATTAA+TGG + chr1.3:56264498-56264517 None:intergenic 15.0%
!!! TTAAATTGATGATGATATAT+CGG + chr1.3:56264830-56264849 None:intergenic 15.0%
!! AACTAAGATATGAATATTCA+AGG + chr1.3:56265039-56265058 None:intergenic 20.0%
!! AACTAAGCATGATAAAAAAT+CGG - chr1.3:56265097-56265116 MS.gene52533:intron 20.0%
!! AATAGAGTTAAAACATACAA+GGG + chr1.3:56264981-56265000 None:intergenic 20.0%
!! ATGTAGATAATTAGGTAAAT+AGG - chr1.3:56264333-56264352 MS.gene52533:CDS 20.0%
!! CAAATTTAAACTTCAATAAC+TGG - chr1.3:56265310-56265329 MS.gene52533:intron 20.0%
!! CAGTTATTGAAGTTTAAATT+TGG + chr1.3:56265312-56265331 None:intergenic 20.0%
!! TAATAGAGTTAAAACATACA+AGG + chr1.3:56264982-56265001 None:intergenic 20.0%
!!! CAATTCATGTATTCTTATTT+CGG - chr1.3:56265506-56265525 MS.gene52533:intron 20.0%
!!! TTTGATGATAATACGAATTA+AGG + chr1.3:56264387-56264406 None:intergenic 20.0%
! AAACAATGTAGCTTATAACT+GGG - chr1.3:56265371-56265390 MS.gene52533:intron 25.0%
! AATTTCAGATGAATGTTAAG+GGG - chr1.3:56265872-56265891 MS.gene52533:CDS 25.0%
! ACTAAGCATGATAAAAAATC+GGG - chr1.3:56265098-56265117 MS.gene52533:intron 25.0%
! ATTGAAGTTTAAATTTGGGT+CGG + chr1.3:56265307-56265326 None:intergenic 25.0%
! CCTAATTATCTACATTTACA+AGG + chr1.3:56264328-56264347 None:intergenic 25.0%
! CCTTGTAAATGTAGATAATT+AGG - chr1.3:56264325-56264344 MS.gene52533:CDS 25.0%
! GAATTTCAGATGAATGTTAA+GGG - chr1.3:56265871-56265890 MS.gene52533:CDS 25.0%
! GATATGAATATTCAAGGAAA+TGG + chr1.3:56265033-56265052 None:intergenic 25.0%
! TGAATTTCAGATGAATGTTA+AGG - chr1.3:56265870-56265889 MS.gene52533:CDS 25.0%
! TTAAACTTCAATAACTGGAA+TGG - chr1.3:56265315-56265334 MS.gene52533:intron 25.0%
!!! CATCGATTTATCGAATATTA+GGG - chr1.3:56264249-56264268 MS.gene52533:CDS 25.0%
!!! CTCTATTATTTCTACTTTCT+TGG - chr1.3:56264994-56265013 MS.gene52533:intron 25.0%
!!! TCATCGATTTATCGAATATT+AGG - chr1.3:56264248-56264267 MS.gene52533:CDS 25.0%
AGAATGCTAATAGTAAGCTT+TGG - chr1.3:56264602-56264621 MS.gene52533:intron 30.0%
AGACAACTTTAGTACTACAA+TGG + chr1.3:56265726-56265745 None:intergenic 30.0%
AGTTTACATGATGAAGGAAT+AGG + chr1.3:56265074-56265093 None:intergenic 30.0%
ATGTTAAGGGGACAAATAAT+TGG - chr1.3:56265884-56265903 MS.gene52533:CDS 30.0%
CAAACAATGTAGCTTATAAC+TGG - chr1.3:56265370-56265389 MS.gene52533:intron 30.0%
CTTCAAAATACAATCTGAGA+GGG + chr1.3:56264892-56264911 None:intergenic 30.0%
GTTGAAAGTTTACATGATGA+AGG + chr1.3:56265080-56265099 None:intergenic 30.0%
TAGTACTAAAGTTGTCTGTT+TGG - chr1.3:56265729-56265748 MS.gene52533:CDS 30.0%
TATGTAGCTGTAGAGAATAT+TGG + chr1.3:56264527-56264546 None:intergenic 30.0%
TCGAAAAGTCTTAGAAAAGA+CGG + chr1.3:56264053-56264072 None:intergenic 30.0%
TTAAGAGTTAACCTAGAATC+AGG + chr1.3:56264749-56264768 None:intergenic 30.0%
TTGAAGTTTAAATTTGGGTC+GGG + chr1.3:56265306-56265325 None:intergenic 30.0%
TTTCAATTGGATAGAAAGAG+TGG - chr1.3:56264644-56264663 MS.gene52533:intron 30.0%
! ATAAACGTGAGAAAAAGTGT+AGG + chr1.3:56264941-56264960 None:intergenic 30.0%
! ATTTCGGTAACTTTTTGTGT+TGG - chr1.3:56265522-56265541 MS.gene52533:intron 30.0%
! TCATCTGAAATTCATCTTCT+AGG + chr1.3:56265864-56265883 None:intergenic 30.0%
!! ATTGATGATGATATATCGGT+CGG + chr1.3:56264826-56264845 None:intergenic 30.0%
!!! ACACTTTTTCTCACGTTTAT+TGG - chr1.3:56264941-56264960 MS.gene52533:intron 30.0%
AACTTCAATAACTGGAATGG+TGG - chr1.3:56265318-56265337 MS.gene52533:intron 35.0%
AATCATCTTGACCACAAGAT+AGG + chr1.3:56265647-56265666 None:intergenic 35.0%
AGGATAGATGATATGTCAGA+AGG + chr1.3:56264115-56264134 None:intergenic 35.0%
ATACTGAGTATTTGGAGACA+AGG + chr1.3:56265620-56265639 None:intergenic 35.0%
ATTCCAGACATCATCAGAAT+CGG + chr1.3:56264083-56264102 None:intergenic 35.0%
CCTTCAAAATACAATCTGAG+AGG + chr1.3:56264893-56264912 None:intergenic 35.0%
GGATAGATGATATGTCAGAA+GGG + chr1.3:56264114-56264133 None:intergenic 35.0%
GTTAAAACATACAAGGGAGA+AGG + chr1.3:56264975-56264994 None:intergenic 35.0%
TAGGACAGTGAGAAATATGT+TGG + chr1.3:56264922-56264941 None:intergenic 35.0%
TCGGGTGTATAGTTAACTTT+GGG + chr1.3:56265288-56265307 None:intergenic 35.0%
TGAAATGCAGAGCTTTCAAT+TGG - chr1.3:56264631-56264650 MS.gene52533:intron 35.0%
TGCTTGAAGGAATAGAAGTT+AGG - chr1.3:56265920-56265939 MS.gene52533:CDS 35.0%
TTCACTTGGAAGAAAAAACG+AGG + chr1.3:56265241-56265260 None:intergenic 35.0%
!! CCTCTCAGATTGTATTTTGA+AGG - chr1.3:56264890-56264909 MS.gene52533:intron 35.0%
!! CTAAACAAGAAGTGTTCACT+TGG + chr1.3:56265255-56265274 None:intergenic 35.0%
!! TTGCTTTTCTTCATCATGTG+TGG - chr1.3:56265560-56265579 MS.gene52533:intron 35.0%
!!! GATCCTACTACTTTGTTTTG+CGG + chr1.3:56265775-56265794 None:intergenic 35.0%
ATGATGGCAATACACTCTTC+AGG + chr1.3:56263992-56264011 None:intergenic 40.0%
CATCATCGATCATGGTCAAA+AGG - chr1.3:56264207-56264226 MS.gene52533:CDS 40.0%
CCTTCTAACCTTCTTGGTAT+GGG + chr1.3:56265138-56265157 None:intergenic 40.0%
CTGTAGAGAATATTGGCATC+TGG + chr1.3:56264520-56264539 None:intergenic 40.0%
GAAAAGCCTTCTAACCTTCT+TGG + chr1.3:56265144-56265163 None:intergenic 40.0%
GCATGATAAAAAATCGGGAG+AGG - chr1.3:56265103-56265122 MS.gene52533:intron 40.0%
GGGACAAATAATTGGAAGAG+AGG - chr1.3:56265892-56265911 MS.gene52533:CDS 40.0%
GTCCTAGTGTAAGAAGTGAT+GGG - chr1.3:56265800-56265819 MS.gene52533:CDS 40.0%
GTCGGGTGTATAGTTAACTT+TGG + chr1.3:56265289-56265308 None:intergenic 40.0%
GTCTCCAAATACTCAGTATG+CGG - chr1.3:56265621-56265640 MS.gene52533:intron 40.0%
GTGATGATCGATATTGCAAC+TGG - chr1.3:56264714-56264733 MS.gene52533:intron 40.0%
TAGATCTCTCTCCATTGCTT+GGG - chr1.3:56264691-56264710 MS.gene52533:intron 40.0%
TGAATATGCCCATACCAAGA+AGG - chr1.3:56265127-56265146 MS.gene52533:intron 40.0%
TGACCATGATCGATGATGAT+AGG + chr1.3:56264205-56264224 None:intergenic 40.0%
TGTCTGTTTGGATCCAAATG+TGG - chr1.3:56265741-56265760 MS.gene52533:CDS 40.0%
TGTTTGGATCCAAATGTGGA+AGG - chr1.3:56265745-56265764 MS.gene52533:CDS 40.0%
! CTTTTCTTCATCATGTGTGG+TGG - chr1.3:56265563-56265582 MS.gene52533:intron 40.0%
! GGTAACTTTTTGTGTTGGAC+AGG - chr1.3:56265527-56265546 MS.gene52533:intron 40.0%
!! AGATAGAGAGCCTTTTTGGT+TGG + chr1.3:56264166-56264185 None:intergenic 40.0%
!! AGGACAGTTCTGAAATCCTT+GGG + chr1.3:56265674-56265693 None:intergenic 40.0%
AAGAGAGGTCTCTTGCTTGA+AGG - chr1.3:56265907-56265926 MS.gene52533:CDS 45.0%
ACACCAACTGTTAACTCCAG+AGG + chr1.3:56265694-56265713 None:intergenic 45.0%
AGATCTCTCTCCATTGCTTG+GGG - chr1.3:56264692-56264711 MS.gene52533:intron 45.0%
AGGATTTCAGAACTGTCCTC+TGG - chr1.3:56265675-56265694 MS.gene52533:intron 45.0%
AGGCCGCAAAACAAAGTAGT+AGG - chr1.3:56265769-56265788 MS.gene52533:CDS 45.0%
CACCCATCACTTCTTACACT+AGG + chr1.3:56265805-56265824 None:intergenic 45.0%
CCCATACCAAGAAGGTTAGA+AGG - chr1.3:56265135-56265154 MS.gene52533:intron 45.0%
CGTCCTAGTGTAAGAAGTGA+TGG - chr1.3:56265799-56265818 MS.gene52533:CDS 45.0%
CGTCCTATCATCATCGATCA+TGG - chr1.3:56264199-56264218 MS.gene52533:CDS 45.0%
CTAGATCTCTCTCCATTGCT+TGG - chr1.3:56264690-56264709 MS.gene52533:intron 45.0%
GAGAATCTGCCAACGTCAAT+AGG + chr1.3:56264031-56264050 None:intergenic 45.0%
GAGTATTTGGAGACAAGGCA+AGG + chr1.3:56265615-56265634 None:intergenic 45.0%
GATGAAGAAAAGCAACCTCG+TGG + chr1.3:56265555-56265574 None:intergenic 45.0%
GCCTTCTAACCTTCTTGGTA+TGG + chr1.3:56265139-56265158 None:intergenic 45.0%
GGATTGTTGTGCCTGATTCT+AGG - chr1.3:56264735-56264754 MS.gene52533:intron 45.0%
GTCAAGATGATTGATGCCCA+AGG - chr1.3:56265655-56265674 MS.gene52533:intron 45.0%
TAGGCCGCATACTGAGTATT+TGG + chr1.3:56265628-56265647 None:intergenic 45.0%
TGCCAACGTTCCAACCAAAA+AGG - chr1.3:56264153-56264172 MS.gene52533:CDS 45.0%
TGGATCCAAATGTGGAAGGT+AGG - chr1.3:56265749-56265768 MS.gene52533:CDS 45.0%
! AGCGAGATAGAGAGCCTTTT+TGG + chr1.3:56264170-56264189 None:intergenic 45.0%
! CTAGTGTAAGAAGTGATGGG+TGG - chr1.3:56265803-56265822 MS.gene52533:CDS 45.0%
! CTGGAGTTAACAGTTGGTGT+CGG - chr1.3:56265694-56265713 MS.gene52533:CDS 45.0%
!! ATCTGAGAGGGCATTCACAA+AGG + chr1.3:56264880-56264899 None:intergenic 45.0%
!! GAGGACAGTTCTGAAATCCT+TGG + chr1.3:56265675-56265694 None:intergenic 45.0%
!! TGTAAGAAGTGATGGGTGGT+TGG - chr1.3:56265807-56265826 MS.gene52533:CDS 45.0%
!! TGTCCTCTGGAGTTAACAGT+TGG - chr1.3:56265688-56265707 MS.gene52533:intron 45.0%
!! TGTGGTGGCTTGGTATTTGT+TGG - chr1.3:56265578-56265597 MS.gene52533:intron 45.0%
!!! AGCCTTTTTGGTTGGAACGT+TGG + chr1.3:56264158-56264177 None:intergenic 45.0%
CAAGTGAAGGAGACTGTGAG+AGG + chr1.3:56264135-56264154 None:intergenic 50.0%
CCAGACATCATCAGAATCGG+CGG + chr1.3:56264080-56264099 None:intergenic 50.0%
CTTCATCATGTGTGGTGGCT+TGG - chr1.3:56265568-56265587 MS.gene52533:intron 50.0%
GGGTGGTTGGAGATTGAGAT+CGG - chr1.3:56265820-56265839 MS.gene52533:CDS 50.0%
GTTGGAACGTTGGCAAGTGA+AGG + chr1.3:56264148-56264167 None:intergenic 50.0%
TCAGTATGCGGCCTATCTTG+TGG - chr1.3:56265633-56265652 MS.gene52533:intron 50.0%
TCGATCATCACCCCAAGCAA+TGG + chr1.3:56264705-56264724 None:intergenic 50.0%
TGTCGACGAGCTTGCATTGT+TGG - chr1.3:56265460-56265479 MS.gene52533:intron 50.0%
! AGTCGTGCGAGAGAGTATGA+TGG + chr1.3:56264008-56264027 None:intergenic 50.0%
! ATCGGAGCGTTCTTCACTTC+AGG - chr1.3:56265838-56265857 MS.gene52533:CDS 50.0%
!! GAGTTAACAGTTGGTGTCGG+AGG - chr1.3:56265697-56265716 MS.gene52533:CDS 50.0%
CGCACGACTCCTATTGACGT+TGG - chr1.3:56264019-56264038 MS.gene52533:CDS 55.0%
TGCGGCCTACCTTCCACATT+TGG + chr1.3:56265757-56265776 None:intergenic 55.0%
TGTGTTGGACAGGTTCCACG+AGG - chr1.3:56265537-56265556 MS.gene52533:intron 55.0%
!! CCGCCGATTCTGATGATGTC+TGG - chr1.3:56264077-56264096 MS.gene52533:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 56263968 56265951 56263968 ID=MS.gene52533
chr1.3 mRNA 56263968 56265951 56263968 ID=MS.gene52533.t1;Parent=MS.gene52533
chr1.3 exon 56265691 56265951 56265691 ID=MS.gene52533.t1.exon1;Parent=MS.gene52533.t1
chr1.3 CDS 56265691 56265951 56265691 ID=cds.MS.gene52533.t1;Parent=MS.gene52533.t1
chr1.3 exon 56265163 56265278 56265163 ID=MS.gene52533.t1.exon2;Parent=MS.gene52533.t1
chr1.3 CDS 56265163 56265278 56265163 ID=cds.MS.gene52533.t1;Parent=MS.gene52533.t1
chr1.3 exon 56263968 56264370 56263968 ID=MS.gene52533.t1.exon3;Parent=MS.gene52533.t1
chr1.3 CDS 56263968 56264370 56263968 ID=cds.MS.gene52533.t1;Parent=MS.gene52533.t1
Gene Sequence

>MS.gene52533

ATGACAAGTTTTGAAGAATTGCCTGAAGAGTGTATTGCCATCATACTCTCTCGCACGACTCCTATTGACGTTGGCAGATTCTCCGTCTTTTCTAAGACTTTTCGATCTGCCGCCGATTCTGATGATGTCTGGAATTGTTTTCTCCCTTCTGACATATCATCTATCCTCTCACAGTCTCCTTCACTTGCCAACGTTCCAACCAAAAAGGCTCTCTATCTCGCTCTCTCTGATCGTCCTATCATCATCGATCATGGTCAAAAGGTATATTATTCTTCATAAATCATCGATTTATCGAATATTAGGGTTTATCATTATATCTAACAATTTTGTTTAATTTGTTTGTGCTTTTAAATTGTGCCTTGTAAATGTAGATAATTAGGTAAATAGGATGAAATATTATATATGCATAAAAATGTCCTTAATTCGTATTATCATCAAATATCTTAAAGATTTCATCAGTTTATGCATTGTGAAATATATTTGTAGAAGAACTCTGCTACTAGAGTTTAATAAAGAAAATAGTGAAACCATTAATTTAAAAAACATAAGCCAGATGCCAATATTCTCTACAGCTACATACGAATGCTCAATTGATTGTTATAAAATATATTGTGATTTTTGAAATGCTTAGTTCAGAATGCTAATAGTAAGCTTTGGAAATATTGAAATGCAGAGCTTTCAATTGGATAGAAAGAGTGGAAAAAAGTGTTACATGCTTTCTGCTAGATCTCTCTCCATTGCTTGGGGTGATGATCGATATTGCAACTGGATTGTTGTGCCTGATTCTAGGTTAACTCTTAACTTTTCAATTTGTTTTATTTTAATTGTGTTCATTAACTTAATGATAAGTAATAACCGACCGATATATCATCATCAATTTAATTAAATCTCAATTGAAAGTTATCTGCACCTTTGTGAATGCCCTCTCAGATTGTATTTTGAAGGTTTGTTCCAACATATTTCTCACTGTCCTACACTTTTTCTCACGTTTATTGGTTTCTTCACCTTCTCCCTTGTATGTTTTAACTCTATTATTTCTACTTTCTTGGCGAATTGTTCATTCCATTTCCTTGAATATTCATATCTTAGTTCACTTGATTGATCCTATTCCTTCATCATGTAAACTTTCAACTAAGCATGATAAAAAATCGGGAGAGGTTGAATATGCCCATACCAAGAAGGTTAGAAGGCTTTTCTTTTATGCAAGATGAAAAACATATCTATAAAGAAGTTTAATAAAAATATTTTCTATTTCTACTTGTCTCACTTTCCTCGTTTTTTCTTCCAAGTGAACACTTCTTGTTTAGACTACGCAATCCCAAAGTTAACTATACACCCGACCCAAATTTAAACTTCAATAACTGGAATGGTGGTCTGCTGTTTGACAAATCTTTGTAGATAACAAACAATGTAGCTTATAACTGGGAAGTTTATCACAATCATTTTCACTTGAAATAATTTAGTTATCATTGGTTTACAACTTTGAAATTTTTGTCGACGAGCTTGCATTGTTGGTGTATCTCTTTCAACTTTTTTCTCAATTCATGTATTCTTATTTCGGTAACTTTTTGTGTTGGACAGGTTCCACGAGGTTGCTTTTCTTCATCATGTGTGGTGGCTTGGTATTTGTTGGATGATAAATATCCTTGCCTTGTCTCCAAATACTCAGTATGCGGCCTATCTTGTGGTCAAGATGATTGATGCCCAAGGATTTCAGAACTGTCCTCTGGAGTTAACAGTTGGTGTCGGAGGTAGCCATTGTAGTACTAAAGTTGTCTGTTTGGATCCAAATGTGGAAGGTAGGCCGCAAAACAAAGTAGTAGGATCGCAACGTCCTAGTGTAAGAAGTGATGGGTGGTTGGAGATTGAGATCGGAGCGTTCTTCACTTCAGGCCTAGAAGATGAATTTCAGATGAATGTTAAGGGGACAAATAATTGGAAGAGAGGTCTCTTGCTTGAAGGAATAGAAGTTAGGCCTAAGTAA
Protein sequence

>MS.gene52533.t1

MTSFEELPEECIAIILSRTTPIDVGRFSVFSKTFRSAADSDDVWNCFLPSDISSILSQSPSLANVPTKKALYLALSDRPIIIDHGQKSFQLDRKSGKKCYMLSARSLSIAWGDDRYCNWIVVPDSRFHEVAFLHHVWWLGICWMINILALSPNTQYAAYLVVKMIDAQGFQNCPLELTVGVGGSHCSTKVVCLDPNVEGRPQNKVVGSQRPSVRSDGWLEIEIGAFFTSGLEDEFQMNVKGTNNWKRGLLLEGIEVRPK