AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49085

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49085.t1 MTR_1g100613 92.500 240 17 1 20 258 5 244 7.09e-164 454
MS.gene49085.t1 MTR_1g100597 82.437 279 44 3 1 278 1 275 1.02e-163 455
MS.gene49085.t1 MTR_1g079180 71.223 278 64 3 1 275 1 265 2.49e-139 393
MS.gene49085.t1 MTR_1g079370 71.739 276 66 3 1 275 1 265 9.67e-139 392
MS.gene49085.t1 MTR_1g079230 68.571 280 82 3 1 278 1 276 1.18e-134 382
MS.gene49085.t1 MTR_1g079260 68.571 280 81 3 1 278 1 275 4.94e-134 380
MS.gene49085.t1 MTR_1g079460 67.376 282 75 3 1 278 1 269 3.73e-130 370
MS.gene49085.t1 MTR_1g079850 67.014 288 81 5 1 278 1 284 1.38e-126 362
MS.gene49085.t1 MTR_1g079200 66.904 281 70 5 1 275 1 264 6.38e-125 357
MS.gene49085.t1 MTR_1g079380 69.565 276 72 3 1 275 1 265 2.89e-123 353
MS.gene49085.t1 MTR_3g006850 61.716 303 89 6 3 278 16 318 1.34e-115 335
MS.gene49085.t1 MTR_1g079250 63.448 290 85 7 1 278 1 281 3.85e-110 320
MS.gene49085.t1 MTR_3g452600 59.783 276 95 3 4 278 10 270 1.90e-105 307
MS.gene49085.t1 MTR_1g026510 57.534 292 104 8 1 278 1 286 5.99e-103 302
MS.gene49085.t1 MTR_1g079410 56.115 278 102 6 5 275 9 273 2.46e-96 284
MS.gene49085.t1 MTR_2g079100 58.242 273 92 4 3 272 8 261 9.72e-95 280
MS.gene49085.t1 MTR_3g450990 53.061 294 102 5 4 278 10 286 4.09e-91 271
MS.gene49085.t1 MTR_1g079350 52.297 283 119 8 1 275 1 275 2.52e-84 254
MS.gene49085.t1 MTR_1g079140 45.791 297 139 7 4 278 13 309 5.82e-80 244
MS.gene49085.t1 MTR_1g079340 50.836 299 110 8 1 275 40 325 7.74e-79 241
MS.gene49085.t1 MTR_1g079400 45.361 291 117 6 4 275 12 279 6.86e-77 235
MS.gene49085.t1 MTR_7g096300 54.545 264 89 8 1 255 1 242 3.82e-75 243
MS.gene49085.t1 MTR_1g078470 63.043 184 51 4 93 275 17 184 4.21e-70 214
MS.gene49085.t1 MTR_7g112550 43.110 283 148 7 2 275 5 283 1.00e-67 212
MS.gene49085.t1 MTR_1g083580 39.427 279 161 5 2 275 6 281 1.74e-64 203
MS.gene49085.t1 MTR_1g079240 57.143 189 60 3 87 275 12 179 1.22e-60 190
MS.gene49085.t1 MTR_7g112560 37.193 285 140 9 4 276 1 258 9.05e-55 178
MS.gene49085.t1 MTR_1g076870 67.164 134 38 3 128 255 2 135 6.91e-54 171
MS.gene49085.t1 MTR_4g023570 35.766 274 137 5 4 275 1 237 2.46e-49 163
MS.gene49085.t1 MTR_1g079210 64.463 121 42 1 129 248 7 127 8.13e-42 140
MS.gene49085.t1 MTR_1g079350 56.391 133 53 2 1 128 1 133 7.28e-41 141
MS.gene49085.t1 MTR_1g079130 44.503 191 88 6 101 275 1 189 6.99e-39 135
MS.gene49085.t1 MTR_7g096310 57.480 127 40 3 67 186 44 163 7.92e-37 129
MS.gene49085.t1 MTR_1g078460 70.115 87 26 0 1 87 64 150 6.22e-36 126
MS.gene49085.t1 MTR_2g042540 60.000 105 40 2 1 103 1 105 6.41e-34 120
MS.gene49085.t1 MTR_3g058640 54.867 113 38 1 128 240 7 106 9.99e-32 114
MS.gene49085.t1 MTR_0113s0040 26.389 288 167 5 2 275 20 276 1.02e-27 108
MS.gene49085.t1 MTR_7g103380 24.912 285 176 6 6 274 21 283 2.80e-27 107
MS.gene49085.t1 MTR_1g101390 28.571 280 169 8 2 274 26 281 7.49e-24 98.2
MS.gene49085.t1 MTR_0002s0020 27.957 279 165 7 6 272 24 278 1.68e-22 94.4
MS.gene49085.t1 MTR_4g102770 27.273 275 172 5 6 274 21 273 3.33e-21 90.9
MS.gene49085.t1 MTR_4g083970 25.862 232 138 5 48 276 91 291 7.90e-19 84.7
MS.gene49085.t1 MTR_5g010800 25.455 275 176 9 6 274 19 270 1.23e-17 81.3
MS.gene49085.t1 MTR_1g079220 54.430 79 23 1 165 243 1 66 1.78e-17 75.5
MS.gene49085.t1 MTR_1g079390 63.265 49 17 1 222 270 8 55 3.91e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49085.t1 AT2G02360 42.545 275 134 6 4 274 14 268 3.91e-69 215
MS.gene49085.t1 AT2G02240 42.647 272 139 4 4 274 61 316 1.12e-63 202
MS.gene49085.t1 AT1G80110 42.804 271 135 7 6 274 3 255 2.81e-63 199
MS.gene49085.t1 AT2G02250 41.455 275 139 6 4 274 47 303 8.36e-61 195
MS.gene49085.t1 AT2G02340 41.219 279 143 6 4 274 36 301 2.15e-59 191
MS.gene49085.t1 AT1G09155 41.197 284 146 8 7 275 3 280 7.91e-59 189
MS.gene49085.t1 AT2G02230 36.039 308 161 6 4 278 32 336 1.40e-58 190
MS.gene49085.t1 AT1G56250 40.071 282 154 7 7 276 4 282 5.12e-54 177
MS.gene49085.t1 AT1G56240 39.286 280 155 7 7 274 4 280 3.53e-52 172
MS.gene49085.t1 AT2G02350 35.612 278 157 7 2 274 22 282 8.28e-50 166
MS.gene49085.t1 AT2G02320 36.462 277 148 7 6 274 42 298 5.16e-48 162
MS.gene49085.t1 AT2G02310 33.452 281 157 5 4 275 45 304 7.28e-46 156
MS.gene49085.t1 AT2G02300 35.587 281 139 9 4 275 34 281 6.44e-42 145
MS.gene49085.t1 AT5G24560 41.146 192 100 4 4 192 3 184 1.71e-41 142
MS.gene49085.t1 AT3G53000 25.862 290 178 6 3 277 20 287 8.77e-27 106
MS.gene49085.t1 AT3G61060 26.259 278 173 8 6 275 27 280 2.71e-24 99.4
MS.gene49085.t1 AT3G61060 26.165 279 173 9 6 275 27 281 6.06e-23 95.9
MS.gene49085.t1 AT1G12710 24.912 285 171 5 3 274 27 281 5.10e-22 93.2
MS.gene49085.t1 AT5G52120 29.617 287 165 11 1 274 18 280 7.35e-22 92.8
MS.gene49085.t1 AT2G02350 31.285 179 104 5 101 274 1 165 3.31e-21 88.6
MS.gene49085.t1 AT1G63090 31.553 206 122 6 3 192 25 227 3.47e-21 90.9
MS.gene49085.t1 AT4G19850 29.474 190 96 5 94 275 17 176 1.23e-17 79.0
MS.gene49085.t1 AT4G19850 29.474 190 96 5 94 275 59 218 1.49e-17 79.7
MS.gene49085.t1 AT1G12710 30.818 159 108 2 3 160 27 184 1.92e-15 75.5
MS.gene49085.t1 AT4G19840 26.971 241 128 9 54 275 34 245 7.18e-14 70.1
MS.gene49085.t1 AT4G19850 41.975 81 41 2 94 168 59 139 1.90e-12 65.5

Find 81 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGCTGCATATTGAGTATT+TGG 0.137691 1.3:+69439095 None:intergenic
CTAGATCTCTTGCCATTACT+TGG 0.259697 1.3:-69440463 MS.gene49085:CDS
TAGCACTAAAATTGTTTGTT+TGG 0.300698 1.3:-69438991 MS.gene49085:CDS
ATGGGAGAGTTCTTCAATTC+AGG 0.321358 1.3:-69438843 MS.gene49085:CDS
CATTATCGACAATGGCAAAA+AGG 0.323000 1.3:-69440644 MS.gene49085:intron
AAGCTGCATATTGAGTATTT+GGG 0.339648 1.3:+69439096 None:intergenic
CCTCCGATTCCGACGCCGTC+TGG 0.342753 1.3:-69440774 MS.gene49085:CDS
TTGCCATTGTCGATAATGAT+AGG 0.347824 1.3:+69440649 None:intergenic
GATCAGATAGGGCAAGATAA+AGG 0.356751 1.3:+69440674 None:intergenic
GGATTGCTATGCCTGATTCC+AGG 0.362529 1.3:-69440415 MS.gene49085:intron
AGACGAAGTTTAGCAACTTC+GGG 0.364842 1.3:+69439167 None:intergenic
TGTAAATGCTCCTTCCAAAA+AGG 0.366929 1.3:-69440698 MS.gene49085:CDS
ATGACAACCGCTACTGGAAC+TGG 0.371010 1.3:-69440436 MS.gene49085:CDS
TATGAGTGTTATCGAGATAA+AGG 0.385187 1.3:-69438802 MS.gene49085:CDS
ATGTATACGAATTCCGCATT+TGG 0.390839 1.3:+69438966 None:intergenic
GAAAATGCAGAGCTTTCAAT+TGG 0.391080 1.3:-69440522 MS.gene49085:intron
TTCTCGAAGGAATAGAAGTT+AGG 0.399223 1.3:-69438755 MS.gene49085:CDS
TTCAAAATGATTGATTCCTA+TGG 0.400444 1.3:-69439065 MS.gene49085:CDS
TTAAGATCAAACCTGGAATC+AGG 0.407191 1.3:+69440404 None:intergenic
TAATGATAGGTTGATCAGAT+AGG 0.414184 1.3:+69440662 None:intergenic
AGGTCAGATAACAGAGTAGT+AGG 0.418025 1.3:-69438912 MS.gene49085:CDS
GGGGTGATGACAACCGCTAC+TGG 0.422453 1.3:-69440442 MS.gene49085:CDS
CGGAGTCGTACGAGACAGTA+TGG 0.434692 1.3:+69440843 None:intergenic
TTATCTCGATAACACTCATA+TGG 0.435546 1.3:+69438805 None:intergenic
TAGATCTCTTGCCATTACTT+GGG 0.440419 1.3:-69440462 MS.gene49085:CDS
GTTGCTTAATTGTCTACCTT+AGG 0.444416 1.3:+69438732 None:intergenic
ATCAGATAGGGCAAGATAAA+GGG 0.457911 1.3:+69440675 None:intergenic
ATTCCAGACGGCGTCGGAAT+CGG 0.462563 1.3:+69440771 None:intergenic
ACGATAGAGTCGACGTCGGA+AGG 0.464466 1.3:+69440739 None:intergenic
AAGACGAAGTTTAGCAACTT+CGG 0.467908 1.3:+69439166 None:intergenic
GAGAATCTACCGGCGTCAAC+CGG 0.472067 1.3:+69440823 None:intergenic
TAGGATTGCCGCGGCCTAGT+TGG 0.483978 1.3:-69438893 MS.gene49085:CDS
TCAAAATGATTGATTCCTAT+GGG 0.503514 1.3:-69439064 MS.gene49085:CDS
CGATAGAGTCGACGTCGGAA+GGG 0.510665 1.3:+69440740 None:intergenic
GTTGGAGAAGTGGTAGGTGG+TGG 0.510954 1.3:-69438875 MS.gene49085:CDS
GACAGGGTTTGAAGATTTAG+CGG 0.514285 1.3:-69440881 MS.gene49085:CDS
GGAGGCAGAAAGGAAAGTCT+TGG 0.523752 1.3:+69440792 None:intergenic
AGGAATAGAAGTTAGGCCTA+AGG 0.524720 1.3:-69438748 MS.gene49085:CDS
TAGGTGGTGGGAGATTGAGA+TGG 0.526412 1.3:-69438862 MS.gene49085:CDS
GATTTATCTGTCGGTGTCGA+AGG 0.528126 1.3:-69439023 MS.gene49085:CDS
AGGTGGTGGGAGATTGAGAT+GGG 0.529389 1.3:-69438861 MS.gene49085:CDS
AATGATAGGTTGATCAGATA+GGG 0.535784 1.3:+69440663 None:intergenic
AGATCTCTTGCCATTACTTG+GGG 0.535874 1.3:-69440461 MS.gene49085:CDS
TTGGAGAAGTGGTAGGTGGT+GGG 0.536804 1.3:-69438874 MS.gene49085:CDS
GGGTTTGAAGATTTAGCGGA+AGG 0.539826 1.3:-69440877 MS.gene49085:CDS
GTGTGGTGGCTCGAAATTCG+TGG 0.547967 1.3:-69439143 MS.gene49085:CDS
CTTCCTGTAGATTTATCTGT+CGG 0.550684 1.3:-69439032 MS.gene49085:CDS
AAGGGCAATTTCTTTCTCGA+AGG 0.553494 1.3:-69438768 MS.gene49085:CDS
CACCTACCACTTCTCCAACT+AGG 0.555718 1.3:+69438879 None:intergenic
GTTGTCATCACCCCAAGTAA+TGG 0.556839 1.3:+69440451 None:intergenic
ACACCGACAGATAAATCTAC+AGG 0.556842 1.3:+69439029 None:intergenic
GATAAAGGCTGGCGAGACAA+AGG 0.558520 1.3:-69438787 MS.gene49085:CDS
ATAAAGGCTGGCGAGACAAA+GGG 0.561504 1.3:-69438786 MS.gene49085:CDS
CTAAACTTCGTCTTGTGTGG+TGG 0.562220 1.3:-69439157 MS.gene49085:CDS
TTGCTAAACTTCGTCTTGTG+TGG 0.565369 1.3:-69439160 MS.gene49085:CDS
CATACTGTCTCGTACGACTC+CGG 0.570766 1.3:-69440842 MS.gene49085:CDS
AGTGTTATCGAGATAAAGGC+TGG 0.573301 1.3:-69438798 MS.gene49085:CDS
CCAGACGGCGTCGGAATCGG+AGG 0.576075 1.3:+69440774 None:intergenic
CGTCGGAATCGGAGGCAGAA+AGG 0.579217 1.3:+69440782 None:intergenic
CCGCGGCCTAGTTGGAGAAG+TGG 0.590220 1.3:-69438885 MS.gene49085:CDS
CAACCTATCATTATCGACAA+TGG 0.600808 1.3:-69440652 MS.gene49085:CDS
AGCTGCATATTGAGTATTTG+GGG 0.601395 1.3:+69439097 None:intergenic
AGGAAGGTTCTCAAACCCAT+AGG 0.604433 1.3:+69439049 None:intergenic
ATATTGAGTATTTGGGGACA+AGG 0.608473 1.3:+69439103 None:intergenic
TGTTTGTTTGGATCCAAATG+CGG 0.611023 1.3:-69438979 MS.gene49085:CDS
TGTGGTGGCTCGAAATTCGT+GGG 0.618223 1.3:-69439142 MS.gene49085:CDS
TTATCTGTCGGTGTCGAAGG+TGG 0.618376 1.3:-69439020 MS.gene49085:CDS
TAGCAATCCAGTTCCAGTAG+CGG 0.650859 1.3:+69440429 None:intergenic
AAAATGATTCCAGACGGCGT+CGG 0.652221 1.3:+69440765 None:intergenic
CTAGTTGGAGAAGTGGTAGG+TGG 0.655061 1.3:-69438878 MS.gene49085:CDS
TTCGTATACATCAGAGACAG+AGG 0.658529 1.3:-69438954 MS.gene49085:CDS
CGTACGACTCCGGTTGACGC+CGG 0.662520 1.3:-69440832 MS.gene49085:CDS
GGCCTAGTTGGAGAAGTGGT+AGG 0.668040 1.3:-69438881 MS.gene49085:CDS
GACGAAGTTTAGCAACTTCG+GGG 0.672634 1.3:+69439168 None:intergenic
GGAAACGACAGAGAATCTAC+CGG 0.672922 1.3:+69440813 None:intergenic
AGGGAGAAAATGATTCCAGA+CGG 0.678403 1.3:+69440759 None:intergenic
ACAGAGTAGTAGGATTGCCG+CGG 0.680550 1.3:-69438902 MS.gene49085:CDS
CGACAGATAAATCTACAGGA+AGG 0.687255 1.3:+69439033 None:intergenic
CCACTTCTCCAACTAGGCCG+CGG 0.703895 1.3:+69438885 None:intergenic
TGAGACGATAGAGTCGACGT+CGG 0.756214 1.3:+69440735 None:intergenic
AGTCGTACGAGACAGTATGG+TGG 0.758050 1.3:+69440846 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATATGATTGTTTATCA+TGG + chr1.3:69439470-69439489 None:intergenic 15.0%
!! AAAATGAAGGGAAAAAAAAT+TGG + chr1.3:69439653-69439672 None:intergenic 20.0%
!! AATAAAAGTGAGAAAATGAA+GGG + chr1.3:69439665-69439684 None:intergenic 20.0%
!! ATATGAAACATTCAATGAAA+TGG + chr1.3:69439756-69439775 None:intergenic 20.0%
!!! AGAAGAAAAATAAGTTGAAA+AGG + chr1.3:69440387-69440406 None:intergenic 20.0%
! AATGAAATGGAATGAACAAT+TGG + chr1.3:69439743-69439762 None:intergenic 25.0%
! AGGATTCATAACATCAAAAA+TGG + chr1.3:69440367-69440386 None:intergenic 25.0%
! CAATAAAAGTGAGAAAATGA+AGG + chr1.3:69439666-69439685 None:intergenic 25.0%
! CAATTCTTGTATTCTTATCT+CGG - chr1.3:69440407-69440426 MS.gene49085:intron 25.0%
! CTAGAGCTTAAAAAAATCAA+AGG + chr1.3:69439625-69439644 None:intergenic 25.0%
! GGAAATAATTTAGCTATCAT+TGG - chr1.3:69440176-69440195 MS.gene49085:intron 25.0%
! TAGCACTAAAATTGTTTGTT+TGG - chr1.3:69440629-69440648 MS.gene49085:intron 25.0%
! TATGCAAGATGAAAAACAAT+GGG - chr1.3:69439829-69439848 MS.gene49085:intron 25.0%
!! ATTCATTGTTTTCTCATGTT+AGG - chr1.3:69439009-69439028 MS.gene49085:CDS 25.0%
!! TCAAAATGATTGATTCCTAT+GGG - chr1.3:69440556-69440575 MS.gene49085:intron 25.0%
!! TTATCATAATCGATTTCACT+TGG - chr1.3:69440155-69440174 MS.gene49085:intron 25.0%
!! TTCAAAATGATTGATTCCTA+TGG - chr1.3:69440555-69440574 MS.gene49085:intron 25.0%
!!! AAACAATTTTAGTGCTACTA+TGG + chr1.3:69440626-69440645 None:intergenic 25.0%
!!! AAACATTTTTAGGTGTGTTT+GGG - chr1.3:69439348-69439367 MS.gene49085:intron 25.0%
!!! CTTCATTTTCTCACTTTTAT+TGG - chr1.3:69439664-69439683 MS.gene49085:intron 25.0%
!!! CTTTGATTTTTTTAAGCTCT+AGG - chr1.3:69439623-69439642 MS.gene49085:intron 25.0%
!!! GAGTTGTTTCAAAGTTTTTT+TGG - chr1.3:69440232-69440251 MS.gene49085:intron 25.0%
AAAAAATGAGGAGAAAGTGA+GGG + chr1.3:69439987-69440006 None:intergenic 30.0%
AAGCTGCATATTGAGTATTT+GGG + chr1.3:69440527-69440546 None:intergenic 30.0%
AGAAAAGTTAAGATCAAACC+TGG + chr1.3:69439226-69439245 None:intergenic 30.0%
ATGCTAGATGTGTAAATTGA+CGG - chr1.3:69439316-69439335 MS.gene49085:intron 30.0%
CTATGCAAGATGAAAAACAA+TGG - chr1.3:69439828-69439847 MS.gene49085:intron 30.0%
TAAAAAGAGTTATCTGACAG+TGG + chr1.3:69440095-69440114 None:intergenic 30.0%
TAAGCTGCATATTGAGTATT+TGG + chr1.3:69440528-69440547 None:intergenic 30.0%
TACACATCTAGCATTCATAA+AGG + chr1.3:69439310-69439329 None:intergenic 30.0%
TATGAGTGTTATCGAGATAA+AGG - chr1.3:69440818-69440837 MS.gene49085:CDS 30.0%
TTATCTCGATAACACTCATA+TGG + chr1.3:69440818-69440837 None:intergenic 30.0%
TTGGGATTGAATAGTCTAAA+AGG + chr1.3:69440026-69440045 None:intergenic 30.0%
TTTAGACTATTCAATCCCAA+AGG - chr1.3:69440026-69440045 MS.gene49085:intron 30.0%
! GATTTTGCAATCGTTTGATA+AGG - chr1.3:69440688-69440707 MS.gene49085:CDS 30.0%
! GCATTTTCAATATACCCAAA+GGG + chr1.3:69439086-69439105 None:intergenic 30.0%
! TCATATGGACTTCATTTTCA+AGG + chr1.3:69440803-69440822 None:intergenic 30.0%
! TGCATTTTCAATATACCCAA+AGG + chr1.3:69439087-69439106 None:intergenic 30.0%
! TTACAATTCTCCTCATTTTC+TGG - chr1.3:69439597-69439616 MS.gene49085:intron 30.0%
! TTGTTTTCTCATGTTAGGTA+AGG - chr1.3:69439014-69439033 MS.gene49085:CDS 30.0%
!! AATGATAGGTTGATCAGATA+GGG + chr1.3:69438960-69438979 None:intergenic 30.0%
!! GTTAGTACTCTTTTGAAAGT+TGG - chr1.3:69440270-69440289 MS.gene49085:intron 30.0%
!! TAATGATAGGTTGATCAGAT+AGG + chr1.3:69438961-69438980 None:intergenic 30.0%
!!! AACATTTTTAGGTGTGTTTG+GGG - chr1.3:69439349-69439368 MS.gene49085:intron 30.0%
!!! CAAACATTTTTAGGTGTGTT+TGG - chr1.3:69439347-69439366 MS.gene49085:intron 30.0%
!!! GATTTTTTTAAGCTCTAGGT+TGG - chr1.3:69439627-69439646 MS.gene49085:intron 30.0%
!!! TTTCTCCTCATTTTTTCTTC+TGG - chr1.3:69439991-69440010 MS.gene49085:intron 30.0%
AAGACGAAGTTTAGCAACTT+CGG + chr1.3:69440457-69440476 None:intergenic 35.0%
AATGAACAATTGGCCAAGAA+AGG + chr1.3:69439733-69439752 None:intergenic 35.0%
ACAGAACAGACATTTGTGTT+TGG + chr1.3:69439570-69439589 None:intergenic 35.0%
AGACATTTGTGTTTGGATTG+AGG + chr1.3:69439563-69439582 None:intergenic 35.0%
AGATGAAAGTTTCCTTGATG+AGG + chr1.3:69439810-69439829 None:intergenic 35.0%
AGCTGCATATTGAGTATTTG+GGG + chr1.3:69440526-69440545 None:intergenic 35.0%
ATATTGAGTATTTGGGGACA+AGG + chr1.3:69440520-69440539 None:intergenic 35.0%
ATCAAAGGAACCAGAAAATG+AGG + chr1.3:69439610-69439629 None:intergenic 35.0%
ATCAGATAGGGCAAGATAAA+GGG + chr1.3:69438948-69438967 None:intergenic 35.0%
ATGAAAGTTTCCTTGATGAG+GGG + chr1.3:69439808-69439827 None:intergenic 35.0%
ATGTATACGAATTCCGCATT+TGG + chr1.3:69440657-69440676 None:intergenic 35.0%
CAACCTATCATTATCGACAA+TGG - chr1.3:69438968-69438987 MS.gene49085:CDS 35.0%
CATTATCGACAATGGCAAAA+AGG - chr1.3:69438976-69438995 MS.gene49085:CDS 35.0%
CATTGTTCTTTCTCCTTTCT+TGG - chr1.3:69439717-69439736 MS.gene49085:intron 35.0%
CTGCTAATAATAAGCCCTTT+GGG - chr1.3:69439069-69439088 MS.gene49085:CDS 35.0%
CTTCCTGTAGATTTATCTGT+CGG - chr1.3:69440588-69440607 MS.gene49085:intron 35.0%
GAAAAAATGAGGAGAAAGTG+AGG + chr1.3:69439988-69440007 None:intergenic 35.0%
GAAAATGCAGAGCTTTCAAT+TGG - chr1.3:69439098-69439117 MS.gene49085:CDS 35.0%
GACATTTGTGTTTGGATTGA+GGG + chr1.3:69439562-69439581 None:intergenic 35.0%
GATGAAAGTTTCCTTGATGA+GGG + chr1.3:69439809-69439828 None:intergenic 35.0%
GCTATCCAGAAGAAAAAATG+AGG + chr1.3:69439999-69440018 None:intergenic 35.0%
GTTAAAACATGCAAGAGAGA+AGG + chr1.3:69439698-69439717 None:intergenic 35.0%
TAGATCTCTTGCCATTACTT+GGG - chr1.3:69439158-69439177 MS.gene49085:CDS 35.0%
TGCTAGATGTGTAAATTGAC+GGG - chr1.3:69439317-69439336 MS.gene49085:intron 35.0%
TGTAAATGCTCCTTCCAAAA+AGG - chr1.3:69438922-69438941 MS.gene49085:CDS 35.0%
TGTTACAGAGGTTCAGATTT+GGG + chr1.3:69440062-69440081 None:intergenic 35.0%
TGTTTGTTTGGATCCAAATG+CGG - chr1.3:69440641-69440660 MS.gene49085:intron 35.0%
TTAAGATCAAACCTGGAATC+AGG + chr1.3:69439219-69439238 None:intergenic 35.0%
TTCTCGAAGGAATAGAAGTT+AGG - chr1.3:69440865-69440884 MS.gene49085:CDS 35.0%
TTGCCATTGTCGATAATGAT+AGG + chr1.3:69438974-69438993 None:intergenic 35.0%
TTGGGTGTATAGTTTCCTTT+GGG + chr1.3:69440044-69440063 None:intergenic 35.0%
TTTGGGTGTATAGTTTCCTT+TGG + chr1.3:69440045-69440064 None:intergenic 35.0%
! GGTCACACTCAAACATTTTT+AGG - chr1.3:69439338-69439357 MS.gene49085:intron 35.0%
! TATCTCGGCAATTTTTGTGT+TGG - chr1.3:69440422-69440441 MS.gene49085:CDS 35.0%
AAGGGCAATTTCTTTCTCGA+AGG - chr1.3:69440852-69440871 MS.gene49085:CDS 40.0%
ACACCGACAGATAAATCTAC+AGG + chr1.3:69440594-69440613 None:intergenic 40.0%
AGACGAAGTTTAGCAACTTC+GGG + chr1.3:69440456-69440475 None:intergenic 40.0%
AGATCTCTTGCCATTACTTG+GGG - chr1.3:69439159-69439178 MS.gene49085:CDS 40.0%
AGGAATAGAAGTTAGGCCTA+AGG - chr1.3:69440872-69440891 MS.gene49085:CDS 40.0%
AGGGAGAAAATGATTCCAGA+CGG + chr1.3:69438864-69438883 None:intergenic 40.0%
AGGTCAGATAACAGAGTAGT+AGG - chr1.3:69440708-69440727 MS.gene49085:CDS 40.0%
AGTGTTATCGAGATAAAGGC+TGG - chr1.3:69440822-69440841 MS.gene49085:CDS 40.0%
ATCTGAGTTCACACTGTTGA+TGG - chr1.3:69439774-69439793 MS.gene49085:intron 40.0%
ATGGGAGAGTTCTTCAATTC+AGG - chr1.3:69440777-69440796 MS.gene49085:CDS 40.0%
ATTCCCGGTACAAGAGATAA+GGG + chr1.3:69439874-69439893 None:intergenic 40.0%
CGACAGATAAATCTACAGGA+AGG + chr1.3:69440590-69440609 None:intergenic 40.0%
CTAGATCTCTTGCCATTACT+TGG - chr1.3:69439157-69439176 MS.gene49085:CDS 40.0%
CTGTTACAGAGGTTCAGATT+TGG + chr1.3:69440063-69440082 None:intergenic 40.0%
GACAGGGTTTGAAGATTTAG+CGG - chr1.3:69438739-69438758 MS.gene49085:CDS 40.0%
GATCAGATAGGGCAAGATAA+AGG + chr1.3:69438949-69438968 None:intergenic 40.0%
GCTGCTAATAATAAGCCCTT+TGG - chr1.3:69439068-69439087 MS.gene49085:CDS 40.0%
GTACAAGAGATAAGGGCTAA+CGG + chr1.3:69439867-69439886 None:intergenic 40.0%
TTAGCCCTTATCTCTTGTAC+CGG - chr1.3:69439867-69439886 MS.gene49085:intron 40.0%
TTCGTATACATCAGAGACAG+AGG - chr1.3:69440666-69440685 MS.gene49085:CDS 40.0%
TTGCTAAACTTCGTCTTGTG+TGG - chr1.3:69440460-69440479 MS.gene49085:CDS 40.0%
! AAGAAAAGCCAGTTGATTCC+CGG + chr1.3:69439889-69439908 None:intergenic 40.0%
! AGATAAAGGGCCTTTTTGGA+AGG + chr1.3:69438935-69438954 None:intergenic 40.0%
!!! TTTTTAGGTGTGTTTGGGGA+TGG - chr1.3:69439353-69439372 MS.gene49085:intron 40.0%
AAAATGATTCCAGACGGCGT+CGG + chr1.3:69438858-69438877 None:intergenic 45.0%
AAATGAGGAGAAAGTGAGGG+AGG + chr1.3:69439984-69440003 None:intergenic 45.0%
AGGAAGGTTCTCAAACCCAT+AGG + chr1.3:69440574-69440593 None:intergenic 45.0%
ATAAAGGCTGGCGAGACAAA+GGG - chr1.3:69440834-69440853 MS.gene49085:CDS 45.0%
CTAAACTTCGTCTTGTGTGG+TGG - chr1.3:69440463-69440482 MS.gene49085:CDS 45.0%
GAACCTCTGTAACAGTGATG+TGG - chr1.3:69440068-69440087 MS.gene49085:intron 45.0%
GACGAAGTTTAGCAACTTCG+GGG + chr1.3:69440455-69440474 None:intergenic 45.0%
GATTCCCGGTACAAGAGATA+AGG + chr1.3:69439875-69439894 None:intergenic 45.0%
GGAAACGACAGAGAATCTAC+CGG + chr1.3:69438810-69438829 None:intergenic 45.0%
GGGTTTGAAGATTTAGCGGA+AGG - chr1.3:69438743-69438762 MS.gene49085:CDS 45.0%
GTTGTCATCACCCCAAGTAA+TGG + chr1.3:69439172-69439191 None:intergenic 45.0%
GTTTCCTTGATGAGGGGAAT+AGG + chr1.3:69439802-69439821 None:intergenic 45.0%
TAGCAATCCAGTTCCAGTAG+CGG + chr1.3:69439194-69439213 None:intergenic 45.0%
TAGCCCTTATCTCTTGTACC+GGG - chr1.3:69439868-69439887 MS.gene49085:intron 45.0%
! CGGCAATTTTTGTGTTGGAC+AGG - chr1.3:69440427-69440446 MS.gene49085:CDS 45.0%
! GGCAAGATAAAGGGCCTTTT+TGG + chr1.3:69438939-69438958 None:intergenic 45.0%
!! GATTTATCTGTCGGTGTCGA+AGG - chr1.3:69440597-69440616 MS.gene49085:intron 45.0%
ACAGAGTAGTAGGATTGCCG+CGG - chr1.3:69440718-69440737 MS.gene49085:CDS 50.0%
AGTCGTACGAGACAGTATGG+TGG + chr1.3:69438777-69438796 None:intergenic 50.0%
ATGACAACCGCTACTGGAAC+TGG - chr1.3:69439184-69439203 MS.gene49085:intron 50.0%
CACCTACCACTTCTCCAACT+AGG + chr1.3:69440744-69440763 None:intergenic 50.0%
CATACTGTCTCGTACGACTC+CGG - chr1.3:69438778-69438797 MS.gene49085:CDS 50.0%
CTAGTTGGAGAAGTGGTAGG+TGG - chr1.3:69440742-69440761 MS.gene49085:CDS 50.0%
CTCTTGTACCGGGAATCAAC+TGG - chr1.3:69439878-69439897 MS.gene49085:intron 50.0%
GAAAGTGAGGGAGGCTGAAA+TGG + chr1.3:69439975-69439994 None:intergenic 50.0%
GATAAAGGCTGGCGAGACAA+AGG - chr1.3:69440833-69440852 MS.gene49085:CDS 50.0%
GGACCACATCACTGTTACAG+AGG + chr1.3:69440074-69440093 None:intergenic 50.0%
GGAGGCAGAAAGGAAAGTCT+TGG + chr1.3:69438831-69438850 None:intergenic 50.0%
GGATCCTATTCCCCTCATCA+AGG - chr1.3:69439795-69439814 MS.gene49085:intron 50.0%
GGATTGCTATGCCTGATTCC+AGG - chr1.3:69439205-69439224 MS.gene49085:intron 50.0%
TAGGTGTGTTTGGGGATGGA+GGG - chr1.3:69439357-69439376 MS.gene49085:intron 50.0%
TGAGACGATAGAGTCGACGT+CGG + chr1.3:69438888-69438907 None:intergenic 50.0%
TGTGGTGGCTCGAAATTCGT+GGG - chr1.3:69440478-69440497 MS.gene49085:CDS 50.0%
TGTTTGGGGATGGAGGGTTT+AGG - chr1.3:69439363-69439382 MS.gene49085:intron 50.0%
TTAGGTGTGTTTGGGGATGG+AGG - chr1.3:69439356-69439375 MS.gene49085:intron 50.0%
TTGGAGAAGTGGTAGGTGGT+GGG - chr1.3:69440746-69440765 MS.gene49085:CDS 50.0%
!! AGGTGGTGGGAGATTGAGAT+GGG - chr1.3:69440759-69440778 MS.gene49085:CDS 50.0%
!! TAGGTGGTGGGAGATTGAGA+TGG - chr1.3:69440758-69440777 MS.gene49085:CDS 50.0%
!! TTATCTGTCGGTGTCGAAGG+TGG - chr1.3:69440600-69440619 MS.gene49085:intron 50.0%
ACGATAGAGTCGACGTCGGA+AGG + chr1.3:69438884-69438903 None:intergenic 55.0%
CGATAGAGTCGACGTCGGAA+GGG + chr1.3:69438883-69438902 None:intergenic 55.0%
CGGAGTCGTACGAGACAGTA+TGG + chr1.3:69438780-69438799 None:intergenic 55.0%
GAGAATCTACCGGCGTCAAC+CGG + chr1.3:69438800-69438819 None:intergenic 55.0%
GGCCTAGTTGGAGAAGTGGT+AGG - chr1.3:69440739-69440758 MS.gene49085:CDS 55.0%
GTGTGGTGGCTCGAAATTCG+TGG - chr1.3:69440477-69440496 MS.gene49085:CDS 55.0%
GTTGGAGAAGTGGTAGGTGG+TGG - chr1.3:69440745-69440764 MS.gene49085:CDS 55.0%
TGGGGATGGAGGGTTTAGGA+GGG - chr1.3:69439367-69439386 MS.gene49085:intron 55.0%
TTGGGGATGGAGGGTTTAGG+AGG - chr1.3:69439366-69439385 MS.gene49085:intron 55.0%
! ATTCCAGACGGCGTCGGAAT+CGG + chr1.3:69438852-69438871 None:intergenic 55.0%
CCACTTCTCCAACTAGGCCG+CGG + chr1.3:69440738-69440757 None:intergenic 60.0%
CGTCGGAATCGGAGGCAGAA+AGG + chr1.3:69438841-69438860 None:intergenic 60.0%
GGAGGGTTTAGGAGGGGGAA+GGG - chr1.3:69439374-69439393 MS.gene49085:intron 60.0%
GGGATGGAGGGTTTAGGAGG+GGG - chr1.3:69439369-69439388 MS.gene49085:intron 60.0%
GGGGATGGAGGGTTTAGGAG+GGG - chr1.3:69439368-69439387 MS.gene49085:intron 60.0%
GGGGTGATGACAACCGCTAC+TGG - chr1.3:69439178-69439197 MS.gene49085:intron 60.0%
GGTTTAGGAGGGGGAAGGGA+GGG - chr1.3:69439378-69439397 MS.gene49085:intron 60.0%
GTTTAGGAGGGGGAAGGGAG+GGG - chr1.3:69439379-69439398 MS.gene49085:intron 60.0%
TAGGATTGCCGCGGCCTAGT+TGG - chr1.3:69440727-69440746 MS.gene49085:CDS 60.0%
TGGAGGGTTTAGGAGGGGGA+AGG - chr1.3:69439373-69439392 MS.gene49085:intron 60.0%
CCGCGGCCTAGTTGGAGAAG+TGG - chr1.3:69440735-69440754 MS.gene49085:CDS 65.0%
CGTACGACTCCGGTTGACGC+CGG - chr1.3:69438788-69438807 MS.gene49085:CDS 65.0%
GGGTTTAGGAGGGGGAAGGG+AGG - chr1.3:69439377-69439396 MS.gene49085:intron 65.0%
CCTCCGATTCCGACGCCGTC+TGG - chr1.3:69438846-69438865 MS.gene49085:CDS 70.0%
! CCAGACGGCGTCGGAATCGG+AGG + chr1.3:69438849-69438868 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 69438737 69440905 69438737 ID=MS.gene49085
chr1.3 mRNA 69438737 69440905 69438737 ID=MS.gene49085.t1;Parent=MS.gene49085
chr1.3 exon 69440645 69440905 69440645 ID=MS.gene49085.t1.exon1;Parent=MS.gene49085.t1
chr1.3 CDS 69440645 69440905 69440645 ID=cds.MS.gene49085.t1;Parent=MS.gene49085.t1
chr1.3 exon 69440416 69440534 69440416 ID=MS.gene49085.t1.exon2;Parent=MS.gene49085.t1
chr1.3 CDS 69440416 69440534 69440416 ID=cds.MS.gene49085.t1;Parent=MS.gene49085.t1
chr1.3 exon 69438737 69439193 69438737 ID=MS.gene49085.t1.exon3;Parent=MS.gene49085.t1
chr1.3 CDS 69438737 69439193 69438737 ID=cds.MS.gene49085.t1;Parent=MS.gene49085.t1
Gene Sequence

>MS.gene49085

ATGACAGGGTTTGAAGATTTAGCGGAAGGATGCATTGCCACCATACTGTCTCGTACGACTCCGGTTGACGCCGGTAGATTCTCTGTCGTTTCCAAGACTTTCCTTTCTGCCTCCGATTCCGACGCCGTCTGGAATCATTTTCTCCCTTCCGACGTCGACTCTATCGTCTCACAGTCATCTTCAGTTGTAAATGCTCCTTCCAAAAAGGCCCTTTATCTTGCCCTATCTGATCAACCTATCATTATCGACAATGGCAAAAAGGTATCATCAGAATTCATTGTTTTCTCATGTTAGGTAAGGCTAAATCAGTGATTATTGATCATCATAATTCGCTGCTAATAATAAGCCCTTTGGGTATATTGAAAATGCAGAGCTTTCAATTGGACAGAAAGAGTGCAAAAAAGTGTTACATGCTTGCTGCTAGATCTCTTGCCATTACTTGGGGTGATGACAACCGCTACTGGAACTGGATTGCTATGCCTGATTCCAGGTTTGATCTTAACTTTTCTATTTGTTTTATTTTAATTGCGTTCATTAACTTAATTAACTCTTAATTGACATTTATCTGCACCTTTATGAATGCTAGATGTGTAAATTGACGGGTCACACTCAAACATTTTTAGGTGTGTTTGGGGATGGAGGGTTTAGGAGGGGGAAGGGAGGGGAGAATTTACTTTTCATTTTTAAATTACGCACACTATGAAATATTTTTTAAAAAGTTAACATATTGCCATGATAAACAATCATATAATACTTCAATCACACTTAGTTTATTTTAAAAAAACATCTTACATAATCTGTAATTTAAAAAAGAAAAGTAAACCCTCAATCCAAACACAAATGTCTGTTCTGTTTCTCTCTTACAATTCTCCTCATTTTCTGGTTCCTTTGATTTTTTTAAGCTCTAGGTTGGCCAATTTTTTTTCCCTTCATTTTCTCACTTTTATTGGTTTCTTTACCTTCTCTCTTGCATGTTTTAACATTGTTCTTTCTCCTTTCTTGGCCAATTGTTCATTCCATTTCATTGAATGTTTCATATCTGAGTTCACACTGTTGATGGATCCTATTCCCCTCATCAAGGAAACTTTCATCTATGCAAGATGAAAAACAATGGGAGATTGAATATGCCGTTAGCCCTTATCTCTTGTACCGGGAATCAACTGGCTTTTCTTCTATGAAAGATGAAAAAACATGAGAAGTTGAATATGCATAAAGAAGTTAAATGAAAATATTTTCCATTTCAGCCTCCCTCACTTTCTCCTCATTTTTTCTTCTGGATAGCACTTCCTTTTAGACTATTCAATCCCAAAGGAAACTATACACCCAAATCTGAACCTCTGTAACAGTGATGTGGTCCACTGTCAGATAACTCTTTTTATAGATAACAAACGATATAACTAACTTATTAAATGAGAAGTTTATCATAATCGATTTCACTTGGAAATAATTTAGCTATCATTGGTACAATATATGATAATTGATGTTTTTATTAGTTGAGTTGTTTCAAAGTTTTTTTGGTAAAAATATTTTGCTGTTAGTACTCTTTTGAAAGTTGGAAACTTCGATTCAACTTTGAAATTTTAGTTGATGAGCTTGCATTGTTAGTATATCTACATGTATAAATATTCCATTTTTGATGTTATGAATCCTTTTCAACTTATTTTTCTTCTCAATTCTTGTATTCTTATCTCGGCAATTTTTGTGTTGGACAGGTTCCCCGAAGTTGCTAAACTTCGTCTTGTGTGGTGGCTCGAAATTCGTGGGATGATTAACAATCTTTCCTTGTCCCCAAATACTCAATATGCAGCTTATCTTGTGTTCAAAATGATTGATTCCTATGGGTTTGAGAACCTTCCTGTAGATTTATCTGTCGGTGTCGAAGGTGGCCATAGTAGCACTAAAATTGTTTGTTTGGATCCAAATGCGGAATTCGTATACATCAGAGACAGAGGATTTTGCAATCGTTTGATAAGGTCAGATAACAGAGTAGTAGGATTGCCGCGGCCTAGTTGGAGAAGTGGTAGGTGGTGGGAGATTGAGATGGGAGAGTTCTTCAATTCAGGCCTTGAAAATGAAGTCCATATGAGTGTTATCGAGATAAAGGCTGGCGAGACAAAGGGCAATTTCTTTCTCGAAGGAATAGAAGTTAGGCCTAAGGTAGACAATTAA
Protein sequence

>MS.gene49085.t1

MTGFEDLAEGCIATILSRTTPVDAGRFSVVSKTFLSASDSDAVWNHFLPSDVDSIVSQSSSVVNAPSKKALYLALSDQPIIIDNGKKSFQLDRKSAKKCYMLAARSLAITWGDDNRYWNWIAMPDSRFPEVAKLRLVWWLEIRGMINNLSLSPNTQYAAYLVFKMIDSYGFENLPVDLSVGVEGGHSSTKIVCLDPNAEFVYIRDRGFCNRLIRSDNRVVGLPRPSWRSGRWWEIEMGEFFNSGLENEVHMSVIEIKAGETKGNFFLEGIEVRPKVDN