AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68122

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68122.t1 MTR_3g118360 96.646 954 25 2 2 955 34 980 0.0 1889
MS.gene68122.t1 MTR_3g118360 96.209 844 25 2 2 845 34 870 0.0 1670
MS.gene68122.t1 MTR_3g118360 96.323 843 24 2 2 844 34 869 0.0 1668
MS.gene68122.t1 MTR_3g118360 99.145 702 6 0 254 955 1 702 0.0 1406
MS.gene68122.t1 MTR_4g074640 35.308 827 445 21 61 829 16 810 1.91e-143 451
MS.gene68122.t1 MTR_4g074670 37.792 471 252 13 61 507 16 469 3.12e-92 306
MS.gene68122.t1 MTR_4g074670 36.213 301 171 8 213 507 2 287 1.18e-46 174
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68122.t1 AT5G22800 73.889 900 232 3 56 953 103 1001 0.0 1381
MS.gene68122.t1 AT5G22800 73.889 900 232 3 56 953 78 976 0.0 1381
MS.gene68122.t1 AT1G50200 37.084 782 407 18 61 786 64 816 1.21e-157 490
MS.gene68122.t1 AT1G50200 37.084 782 407 18 61 786 64 816 2.63e-157 489

Find 215 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGCTTATCTTTGTGAAAA+AGG 0.152053 3.4:+100241473 MS.gene68122:CDS
GTACCTGACACTGAATTTAT+TGG 0.154294 3.4:+100235683 MS.gene68122:CDS
ACACTAATCCATAATCTTTC+TGG 0.181990 3.4:-100234140 None:intergenic
CAATTCAAACCTATCTTTCT+TGG 0.208066 3.4:+100233921 MS.gene68122:CDS
AATACTTTCTGAGCAATTAT+AGG 0.215096 3.4:-100235284 None:intergenic
ATTCAGGCAGGTAAATTTAT+TGG 0.228211 3.4:+100241335 MS.gene68122:CDS
TACTCCAGGGGTGGTTGGTC+AGG 0.230750 3.4:+100241310 MS.gene68122:CDS
GTTTGCCTCCAGAAAGATTA+TGG 0.233077 3.4:+100234132 MS.gene68122:CDS
AAGTCAGTTTCATAGTTATT+TGG 0.248121 3.4:-100234905 None:intergenic
AAGTGCCGCAGAATATTTAA+TGG 0.255077 3.4:+100241211 MS.gene68122:CDS
ATCCTCACAGTTACTTCTTC+AGG 0.258382 3.4:-100239038 None:intergenic
GACCTCTCAAGCTGGTTCTT+TGG 0.278405 3.4:+100236265 MS.gene68122:CDS
ATAGATGTTCAGAAGTCTTT+TGG 0.278583 3.4:+100235908 MS.gene68122:CDS
GGCAGGTTGCTTGGTATAAA+AGG 0.291942 3.4:+100235227 MS.gene68122:CDS
TCTATGTTCTTCTGCTTTAG+AGG 0.298105 3.4:-100234597 None:intergenic
TGGGAGGAAGCCTGAAAATT+TGG 0.299980 3.4:+100241418 MS.gene68122:CDS
TCCCCGTTGACCGAATTAAA+AGG 0.301895 3.4:+100234299 MS.gene68122:CDS
CCTCCTGATTCAGCATAAAA+TGG 0.304165 3.4:-100235815 None:intergenic
GCTGAATCAGGAGGACAAAT+TGG 0.307268 3.4:+100235824 MS.gene68122:CDS
GGTGGTTGGTCAGGGAATTC+AGG 0.315604 3.4:+100241319 MS.gene68122:CDS
CTACCCGTAACTCCTCTAAA+AGG 0.316525 3.4:-100239067 None:intergenic
GACTTATCTACCATTGAGTT+TGG 0.319082 3.4:+100234110 MS.gene68122:CDS
ACTGTTCTCCTCACCATTGC+TGG 0.323081 3.4:+100233891 MS.gene68122:CDS
GAAGGAGCAATTGCAATGTT+TGG 0.329419 3.4:+100236437 MS.gene68122:CDS
AAGCAGAAGAACATAGATAC+TGG 0.331732 3.4:+100234603 MS.gene68122:CDS
ACTACAAGCTGCGCTTAAAA+AGG 0.334338 3.4:+100236229 MS.gene68122:CDS
CAACCTCTAGAACACCCTTT+TGG 0.351759 3.4:-100235958 None:intergenic
TAACCCCTCCCAACGTTTGA+TGG 0.352810 3.4:-100235167 None:intergenic
GTGATCCAGCCGTACAGGTT+AGG 0.360098 3.4:+100235756 MS.gene68122:CDS
CAGGATACTAGTTGAGTGTT+TGG 0.364396 3.4:+100241163 MS.gene68122:intron
AGGAGAACAGTGGGGTCATC+TGG 0.366681 3.4:-100233879 None:intergenic
AACAAGCTATTCAATGGGCT+TGG 0.372665 3.4:+100234087 MS.gene68122:CDS
TTACCTGTTGAGACCATAAC+TGG 0.378573 3.4:-100234187 None:intergenic
CTTTCAGATACCTCTTTGTC+TGG 0.392755 3.4:-100234991 None:intergenic
GAATTAGCAAATATTTCATA+TGG 0.402355 3.4:+100234956 MS.gene68122:CDS
GTCAAGCTTGCCATTGGAAG+CGG 0.403782 3.4:+100235641 MS.gene68122:CDS
CATAGATACTGGGCTTGGTC+TGG 0.405421 3.4:+100234614 MS.gene68122:CDS
ATCATGAGACTTTCCAGTTA+TGG 0.406423 3.4:+100234174 MS.gene68122:CDS
GTAGCCAGCCTAGTGGTAAA+TGG 0.407529 3.4:+100235734 MS.gene68122:CDS
CCCACCAGCCTGAGCGAAGT+TGG 0.409388 3.4:-100241399 None:intergenic
TGGTGGCATTTGATCGTCTC+AGG 0.410015 3.4:+100236285 MS.gene68122:CDS
GTACGTGTTGTAGAGGTTCC+TGG 0.424531 3.4:+100237591 MS.gene68122:CDS
ATTATCTCCACGACAGCCTT+AGG 0.424823 3.4:-100235887 None:intergenic
TTTCCAGTTATGGTCTCAAC+AGG 0.427549 3.4:+100234184 MS.gene68122:CDS
ACTCCAGGGGTGGTTGGTCA+GGG 0.433011 3.4:+100241311 MS.gene68122:CDS
CATACCTTCTTTGAGATGCT+TGG 0.433272 3.4:+100234035 MS.gene68122:CDS
TTATGTGGTGGTGGAGGAGG+TGG 0.436304 3.4:+100241371 MS.gene68122:CDS
TGGGTGAAGAGGATAACTTT+TGG 0.438035 3.4:+100234323 MS.gene68122:CDS
TCAGAGCAGGGTATTGCATC+TGG 0.441838 3.4:+100237684 MS.gene68122:CDS
GGAGGACAAATTGGAGATCA+TGG 0.446126 3.4:+100235833 MS.gene68122:CDS
CTCTCAAGCTGGTTCTTTGG+TGG 0.451414 3.4:+100236268 MS.gene68122:CDS
TTCAGAGGCCTTCTCTATGA+TGG 0.452809 3.4:-100234936 None:intergenic
AGGCGAATAGAAGCTGTAGC+TGG 0.454459 3.4:+100237711 MS.gene68122:CDS
ATGCTTGGTAACTTCAGCTT+TGG 0.456959 3.4:+100234050 MS.gene68122:CDS
ATTGTCCTGGGCTCATGTCC+AGG 0.459686 3.4:+100241257 MS.gene68122:CDS
TATCCAATAAATTCAGTGTC+AGG 0.460034 3.4:-100235686 None:intergenic
AGGGTATTGCATCTGGTGTC+AGG 0.461565 3.4:+100237691 MS.gene68122:CDS
CTTCAACTCCTCAAGGATAC+GGG 0.462475 3.4:-100235351 None:intergenic
TGATCCAGCCGTACAGGTTA+GGG 0.465370 3.4:+100235757 MS.gene68122:CDS
AGTAATACTTCTGAAATTCG+TGG 0.467614 3.4:+100237648 MS.gene68122:CDS
TCACAAGGGTACAGTCCAAA+AGG 0.467810 3.4:+100235943 MS.gene68122:CDS
GGAACTGTACCTTGCTTGGC+TGG 0.471150 3.4:+100235467 MS.gene68122:CDS
ATTCCCTGACCAACCACCCC+TGG 0.472687 3.4:-100241314 None:intergenic
CATGGCTCGCATTCTTCAAA+AGG 0.478575 3.4:+100234641 MS.gene68122:CDS
ACAAGCTATTCAATGGGCTT+GGG 0.479029 3.4:+100234088 MS.gene68122:CDS
GGTAGCAAGAAACGAAAATT+CGG 0.480512 3.4:+100239085 MS.gene68122:CDS
TCTTCAACTCCTCAAGGATA+CGG 0.483308 3.4:-100235352 None:intergenic
TCCAACTTCGCTCAGGCTGG+TGG 0.485257 3.4:+100241398 MS.gene68122:CDS
GGGTGTTGAAAGCAGGATCT+TGG 0.487566 3.4:+100234498 MS.gene68122:intron
AAATTATGTGGTGGTGGAGG+AGG 0.487864 3.4:+100241368 MS.gene68122:CDS
TTTCTGCAAGCTCGCTATCA+AGG 0.488467 3.4:-100236334 None:intergenic
ACATCGGCGTATCCCCTCTC+AGG 0.490257 3.4:-100234403 None:intergenic
GTTGACCGAATTAAAAGGTT+GGG 0.490401 3.4:+100234304 MS.gene68122:CDS
AGAGCAAGGACCGCAGGGAC+CGG 0.491524 3.4:-100234359 None:intergenic
GTTCGTACAGGCAGGTTGCT+TGG 0.494701 3.4:+100235218 MS.gene68122:CDS
TTGGTATAAAAGGTGATGGT+AGG 0.500325 3.4:+100235237 MS.gene68122:CDS
AAATGGTGATCCAGCCGTAC+AGG 0.500392 3.4:+100235751 MS.gene68122:CDS
ATCAGAAGAGTTGTTCGTAC+AGG 0.503028 3.4:+100235206 MS.gene68122:CDS
AATAATAATAATTGTGTGAG+TGG 0.505763 3.4:+100233780 MS.gene68122:CDS
GGCGAATAGAAGCTGTAGCT+GGG 0.506427 3.4:+100237712 MS.gene68122:CDS
GAAGAGTTGTTCGTACAGGC+AGG 0.512237 3.4:+100235210 MS.gene68122:CDS
TGATACTTATGGTTTCCCTA+TGG 0.515111 3.4:+100235511 MS.gene68122:CDS
CATTGTTGATATCATTAGTA+CGG 0.517063 3.4:-100233992 None:intergenic
TCAGAGGCCTTCTCTATGAT+GGG 0.518722 3.4:-100234935 None:intergenic
TGGTATAAAAGGTGATGGTA+GGG 0.518928 3.4:+100235238 MS.gene68122:CDS
GCGTTGTACCATCAAACGTT+GGG 0.520683 3.4:+100235159 MS.gene68122:CDS
GCAAAATTATGTGGTGGTGG+AGG 0.520739 3.4:+100241365 MS.gene68122:CDS
GTACCATCAAACGTTGGGAG+GGG 0.521676 3.4:+100235164 MS.gene68122:CDS
GTTACCAAGCATCTCAAAGA+AGG 0.522266 3.4:-100234039 None:intergenic
CAAAAGGGTGTTCTAGAGGT+TGG 0.522615 3.4:+100235959 MS.gene68122:CDS
CTCTGATTTAGCTTTCTCGA+GGG 0.522771 3.4:-100241447 None:intergenic
AATTTCAGAGCAAGGACCGC+AGG 0.525249 3.4:-100234365 None:intergenic
ATTGTGTTTCTCATCTCTGA+TGG 0.528181 3.4:+100235137 MS.gene68122:CDS
CCTTCCCTAACCTGTACGGC+TGG 0.530381 3.4:-100235761 None:intergenic
ACTAAGAAATCTTTCCCGTA+TGG 0.530405 3.4:-100233808 None:intergenic
AGGTCCAACTTCGCTCAGGC+TGG 0.530546 3.4:+100241395 MS.gene68122:CDS
CTTCACCTGGACATGAGCCC+AGG 0.531541 3.4:-100241262 None:intergenic
TGGGCTTGGTCTGGAGCGCA+TGG 0.533587 3.4:+100234623 MS.gene68122:CDS
GCTTCGATTTGTGCAGACGC+TGG 0.536077 3.4:+100235382 MS.gene68122:CDS
TGGAAGGTCCAACTTCGCTC+AGG 0.537180 3.4:+100241391 MS.gene68122:CDS
AATACTGTCAAGCTTGCCAT+TGG 0.538095 3.4:+100235635 MS.gene68122:CDS
GAGCCCAGGACAATAGCTGC+TGG 0.539000 3.4:-100241248 None:intergenic
GAATTGAAGCATTCCAGCAA+TGG 0.539001 3.4:-100233904 None:intergenic
CTGGATCACCATTTACCACT+AGG 0.539915 3.4:-100235742 None:intergenic
GGAGGGGTTATGTAGTTCGA+CGG 0.540107 3.4:+100235180 MS.gene68122:CDS
CGTTGACCGAATTAAAAGGT+TGG 0.540395 3.4:+100234303 MS.gene68122:CDS
CTGGGCTCATGTCCAGGTGA+AGG 0.540402 3.4:+100241263 MS.gene68122:CDS
AAGAACATAGATACTGGGCT+TGG 0.541349 3.4:+100234609 MS.gene68122:CDS
AGACTAACCTTCCCTTCACC+TGG 0.541694 3.4:-100241275 None:intergenic
GGAAAGTCTCATGATCGTCT+TGG 0.542083 3.4:-100234166 None:intergenic
TTTAAAGTAATTTCAGAGCA+GGG 0.545061 3.4:+100237672 MS.gene68122:CDS
AGATCCAGCAGCTATTGTCC+TGG 0.546412 3.4:+100241244 MS.gene68122:CDS
TCAAGCTTGCCATTGGAAGC+GGG 0.546840 3.4:+100235642 MS.gene68122:CDS
AATTAAAAGGTTGGGTGAAG+AGG 0.549692 3.4:+100234312 MS.gene68122:CDS
GATCCAGCAGCTATTGTCCT+GGG 0.550834 3.4:+100241245 MS.gene68122:CDS
TTATGCTTCCCGTGGTCACA+AGG 0.553002 3.4:+100233833 MS.gene68122:CDS
GTTGGTCAGGGAATTCAGGC+AGG 0.558788 3.4:+100241323 MS.gene68122:CDS
CACAAGGGTACAGTCCAAAA+GGG 0.558794 3.4:+100235944 MS.gene68122:CDS
GGTCAGGAGACCTCTCAAGC+TGG 0.560850 3.4:+100236257 MS.gene68122:CDS
ATATCAAGTGCTGAAAGAAG+TGG 0.561924 3.4:+100235446 MS.gene68122:CDS
GTAGCACGAGGGACTTGTCT+AGG 0.562012 3.4:-100233951 None:intergenic
TGGAGGCAAACCAAACTCAA+TGG 0.563536 3.4:-100234120 None:intergenic
CCAACTTCGCTCAGGCTGGT+GGG 0.563594 3.4:+100241399 MS.gene68122:CDS
GCAGTAGCAGCAGGAGCAGG+AGG 0.564687 3.4:+100233645 MS.gene68122:CDS
ACTCCCTTCCCTAACCTGTA+CGG 0.564727 3.4:-100235765 None:intergenic
TGGCAATATATTTGTTCACA+AGG 0.567482 3.4:+100235928 MS.gene68122:CDS
TTAGCAGTAGCAGCAGGAGC+AGG 0.567552 3.4:+100233642 MS.gene68122:CDS
ATCAGCAAGAGGCATCACTT+TGG 0.568344 3.4:-100236405 None:intergenic
AATATTTGTCCCGCTTCCAA+TGG 0.570926 3.4:-100235651 None:intergenic
ATAGCAAAATTATGTGGTGG+TGG 0.571171 3.4:+100241362 MS.gene68122:CDS
TCATAATAAATTTCAGAGCA+AGG 0.571821 3.4:-100234373 None:intergenic
AAAGTTGAGGCAACGAGCCA+AGG 0.572093 3.4:+100236009 MS.gene68122:CDS
TGTACCATCAAACGTTGGGA+GGG 0.573784 3.4:+100235163 MS.gene68122:CDS
CACCAAAGAACCAGCTTGAG+AGG 0.573822 3.4:-100236267 None:intergenic
GTTACATTAGCAGTAGCAGC+AGG 0.574530 3.4:+100233636 MS.gene68122:CDS
CTTCTCTCTTCAACTCCTCA+AGG 0.574968 3.4:-100235358 None:intergenic
GTGTGAGTGGAGATTCCATA+CGG 0.575858 3.4:+100233793 MS.gene68122:CDS
TTGCGACGATGATGTACAGA+AGG 0.575956 3.4:-100233718 None:intergenic
ATCACCATTTACCACTAGGC+TGG 0.578306 3.4:-100235738 None:intergenic
TGAAATATTTGCTAATTCAG+AGG 0.579198 3.4:-100234951 None:intergenic
ACTAATGATATCAACAATGT+TGG 0.579654 3.4:+100233996 MS.gene68122:CDS
TATGGAAATAACAAAAGAAG+TGG 0.579964 3.4:+100235529 MS.gene68122:CDS
CTCATGTCCAGGTGAAGGGA+AGG 0.580776 3.4:+100241268 MS.gene68122:CDS
GTGGTGGTGGAGGAGGTGGA+AGG 0.581825 3.4:+100241375 MS.gene68122:CDS
TGGGCTCATGTCCAGGTGAA+GGG 0.582195 3.4:+100241264 MS.gene68122:CDS
AGCAGAAGAACATAGATACT+GGG 0.583073 3.4:+100234604 MS.gene68122:CDS
AGGAAGGACCTTGTGACCAC+GGG 0.584439 3.4:-100233841 None:intergenic
GCAATGGTGAGGAGAACAGT+GGG 0.584917 3.4:-100233888 None:intergenic
AAGTGGAACTGTACCTTGCT+TGG 0.584929 3.4:+100235463 MS.gene68122:CDS
TTGTACCATCAAACGTTGGG+AGG 0.589872 3.4:+100235162 MS.gene68122:CDS
GGTATAAAAGGTGATGGTAG+GGG 0.590969 3.4:+100235239 MS.gene68122:CDS
GGCAATATATTTGTTCACAA+GGG 0.592455 3.4:+100235929 MS.gene68122:CDS
TGGGGCATTCAAAACAGTAC+AGG 0.592610 3.4:+100239168 MS.gene68122:CDS
CAAACAAGTGGAAGCAACAG+TGG 0.594498 3.4:+100235982 MS.gene68122:CDS
GCTGATCAACGGATGGATTG+AGG 0.596209 3.4:+100236364 MS.gene68122:CDS
AGAGGTTCCTGGAGTATCGA+TGG 0.596716 3.4:+100237602 MS.gene68122:CDS
TTGCTTGGTATAAAAGGTGA+TGG 0.598004 3.4:+100235233 MS.gene68122:CDS
AGTCCAAAAGGGTGTTCTAG+AGG 0.604342 3.4:+100235955 MS.gene68122:CDS
AGGAAGCCTGAAAATTTGGC+TGG 0.604884 3.4:+100241422 MS.gene68122:CDS
TTTGTGCAGACGCTGGAGAG+AGG 0.605643 3.4:+100235389 MS.gene68122:CDS
TGTTTCCATTAAATATTCTG+CGG 0.605968 3.4:-100241216 None:intergenic
CAAAGTTCCATCGATACTCC+AGG 0.606263 3.4:-100237609 None:intergenic
CGCTATCAAGGAGTGGACGG+TGG 0.606601 3.4:-100236322 None:intergenic
AGGATCTTGGTGATGATACA+CGG 0.607079 3.4:+100234511 MS.gene68122:intron
GATTCTCCCCATCATAGAGA+AGG 0.608580 3.4:+100234928 MS.gene68122:CDS
GGACAGATAGCAAAATTATG+TGG 0.609892 3.4:+100241356 MS.gene68122:CDS
GCTCTGATTTAGCTTTCTCG+AGG 0.610643 3.4:-100241448 None:intergenic
GAAATCGAACTGCTGATCAA+CGG 0.611385 3.4:+100236353 MS.gene68122:CDS
CCAGCCGTACAGGTTAGGGA+AGG 0.611758 3.4:+100235761 MS.gene68122:CDS
GAACGAGAGAAGCACTAGGA+AGG 0.612505 3.4:-100233857 None:intergenic
TCAGGAACATACTTTGAGAG+TGG 0.613102 3.4:-100237790 None:intergenic
TCGAACTGCTGATCAACGGA+TGG 0.614833 3.4:+100236357 MS.gene68122:CDS
GAATCAACCTAAGGCTGTCG+TGG 0.615063 3.4:+100235880 MS.gene68122:CDS
GGCGTTGTACCATCAAACGT+TGG 0.615289 3.4:+100235158 MS.gene68122:CDS
ATTTCAGAGCAAGGACCGCA+GGG 0.616018 3.4:-100234364 None:intergenic
AGAGGGTGAGAATCAACCTA+AGG 0.616634 3.4:+100235871 MS.gene68122:CDS
TAGGAAGGACCTTGTGACCA+CGG 0.617961 3.4:-100233842 None:intergenic
ACTTCGCTCAGGCTGGTGGG+AGG 0.619056 3.4:+100241402 MS.gene68122:CDS
GGAGTATCGATGGAACTTTG+TGG 0.622057 3.4:+100237612 MS.gene68122:CDS
CTAATCCATAATCTTTCTGG+AGG 0.622240 3.4:-100234137 None:intergenic
GCTCGCTATCAAGGAGTGGA+CGG 0.625144 3.4:-100236325 None:intergenic
TGAAGGGAAGGTTAGTCTAG+TGG 0.626264 3.4:+100241280 MS.gene68122:CDS
TCTAGAGGTTGGCAAACAAG+TGG 0.626675 3.4:+100235970 MS.gene68122:CDS
AGCAATGGTGAGGAGAACAG+TGG 0.627721 3.4:-100233889 None:intergenic
GGTTAGGGAAGGGAGTAATG+TGG 0.629772 3.4:+100235772 MS.gene68122:CDS
GTGTTTGGATGATGTTGATG+CGG 0.629997 3.4:+100241178 MS.gene68122:CDS
GGTGTGAGTATTGACATGAA+TGG 0.634865 3.4:+100235563 MS.gene68122:CDS
TATCATACTACTTACAACAT+CGG 0.636211 3.4:-100234419 None:intergenic
GAAGCATTCCAGCAATGGTG+AGG 0.639478 3.4:-100233899 None:intergenic
GCAAGCTCGCTATCAAGGAG+TGG 0.639731 3.4:-100236329 None:intergenic
CGTTGTGAAGTAGTAGCACG+AGG 0.644204 3.4:-100233963 None:intergenic
GATGGTAGGGGAGACCCTGA+AGG 0.644605 3.4:+100235251 MS.gene68122:CDS
ACTGCAGGTACGTGTTGTAG+AGG 0.644859 3.4:+100237584 MS.gene68122:intron
TATCGATGGAACTTTGTGGT+GGG 0.645529 3.4:+100237616 MS.gene68122:CDS
CAGCCGTACAGGTTAGGGAA+GGG 0.646194 3.4:+100235762 MS.gene68122:CDS
AGCGGAGTCACCGGTCCCTG+CGG 0.646446 3.4:+100234349 MS.gene68122:CDS
TAGAGCACCCCGTATCCTTG+AGG 0.648075 3.4:+100235343 MS.gene68122:CDS
CAACAGTGGATGTAAAGTTG+AGG 0.654944 3.4:+100235996 MS.gene68122:CDS
AGCTTGTAGTAAATGAGTAG+CGG 0.656398 3.4:-100236216 None:intergenic
TGTGAGTGGAGATTCCATAC+GGG 0.656667 3.4:+100233794 MS.gene68122:CDS
CAGAGGCCTTCTCTATGATG+GGG 0.657776 3.4:-100234934 None:intergenic
GCGAATAGAAGCTGTAGCTG+GGG 0.661208 3.4:+100237713 MS.gene68122:CDS
TATCTGATGACCAGACAAAG+AGG 0.661772 3.4:+100234981 MS.gene68122:CDS
TTCAACTCCTCAAGGATACG+GGG 0.663705 3.4:-100235350 None:intergenic
AAAGAAGGTATGATGACGTG+AGG 0.668436 3.4:-100234024 None:intergenic
ACATCTTCACCAGCCAAGCA+AGG 0.684496 3.4:-100235476 None:intergenic
AAAGAAGTGGCTGAAGAACG+CGG 0.692053 3.4:+100235542 MS.gene68122:CDS
TGCGACGATGATGTACAGAA+GGG 0.698896 3.4:-100233717 None:intergenic
AGCAATTGTAGCCAGCCTAG+TGG 0.702322 3.4:+100235727 MS.gene68122:CDS
GTATCGATGGAACTTTGTGG+TGG 0.704292 3.4:+100237615 MS.gene68122:CDS
TCTGGAACGAGAGAAGCACT+AGG 0.708872 3.4:-100233861 None:intergenic
AACCTGAAGAAGTAACTGTG+AGG 0.719116 3.4:+100239036 MS.gene68122:CDS
CAGATAGCAAAATTATGTGG+TGG 0.721808 3.4:+100241359 MS.gene68122:CDS
GTTGTGAAGTAGTAGCACGA+GGG 0.763537 3.4:-100233962 None:intergenic
CAATGGTGAGGAGAACAGTG+GGG 0.772634 3.4:-100233887 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.4 gene 100233630 100241502 100233630 ID=MS.gene68122
chr3.4 mRNA 100233630 100241502 100233630 ID=MS.gene68122.t1;Parent=MS.gene68122
chr3.4 exon 100233630 100234205 100233630 ID=MS.gene68122.t1.exon1;Parent=MS.gene68122.t1
chr3.4 CDS 100233630 100234205 100233630 ID=cds.MS.gene68122.t1;Parent=MS.gene68122.t1
chr3.4 exon 100234292 100234426 100234292 ID=MS.gene68122.t1.exon2;Parent=MS.gene68122.t1
chr3.4 CDS 100234292 100234426 100234292 ID=cds.MS.gene68122.t1;Parent=MS.gene68122.t1
chr3.4 exon 100234513 100234662 100234513 ID=MS.gene68122.t1.exon3;Parent=MS.gene68122.t1
chr3.4 CDS 100234513 100234662 100234513 ID=cds.MS.gene68122.t1;Parent=MS.gene68122.t1
chr3.4 exon 100234902 100235012 100234902 ID=MS.gene68122.t1.exon4;Parent=MS.gene68122.t1
chr3.4 CDS 100234902 100235012 100234902 ID=cds.MS.gene68122.t1;Parent=MS.gene68122.t1
chr3.4 exon 100235113 100236030 100235113 ID=MS.gene68122.t1.exon5;Parent=MS.gene68122.t1
chr3.4 CDS 100235113 100236030 100235113 ID=cds.MS.gene68122.t1;Parent=MS.gene68122.t1
chr3.4 exon 100236206 100236478 100236206 ID=MS.gene68122.t1.exon6;Parent=MS.gene68122.t1
chr3.4 CDS 100236206 100236478 100236206 ID=cds.MS.gene68122.t1;Parent=MS.gene68122.t1
chr3.4 exon 100237591 100237800 100237591 ID=MS.gene68122.t1.exon7;Parent=MS.gene68122.t1
chr3.4 CDS 100237591 100237800 100237591 ID=cds.MS.gene68122.t1;Parent=MS.gene68122.t1
chr3.4 exon 100239032 100239189 100239032 ID=MS.gene68122.t1.exon8;Parent=MS.gene68122.t1
chr3.4 CDS 100239032 100239189 100239032 ID=cds.MS.gene68122.t1;Parent=MS.gene68122.t1
chr3.4 exon 100241166 100241502 100241166 ID=MS.gene68122.t1.exon9;Parent=MS.gene68122.t1
chr3.4 CDS 100241166 100241502 100241166 ID=cds.MS.gene68122.t1;Parent=MS.gene68122.t1
Gene Sequence

>MS.gene68122

ATGAATGTTACATTAGCAGTAGCAGCAGGAGCAGGAGGATTTATCTCTTTCAAACAACTACATCTACGTCGTAGTCGTCGTCTTCTTCCCTTCTGTACATCATCGTCGCAAGACAATGATAATAATAATAATAATAATAATAATAATAATAATAATAATAATTGTGTGAGTGGAGATTCCATACGGGAAAGATTTCTTAGTTTTTATGCTTCCCGTGGTCACAAGGTCCTTCCTAGTGCTTCTCTCGTTCCAGATGACCCCACTGTTCTCCTCACCATTGCTGGAATGCTTCAATTCAAACCTATCTTTCTTGGAAAACTTCCTAGACAAGTCCCTCGTGCTACTACTTCACAACGCTGCATCCGTACTAATGATATCAACAATGTTGGTCATACCTCACGTCATCATACCTTCTTTGAGATGCTTGGTAACTTCAGCTTTGGTGATTACTTCAAAAAACAAGCTATTCAATGGGCTTGGGACTTATCTACCATTGAGTTTGGTTTGCCTCCAGAAAGATTATGGATTAGTGTTTACCAAGACGATCATGAGACTTTCCAGTTATGGTCTCAACAGGTAATTACATCACAATCACATTTCCTTTTAATTACCCCTCTCCTCTTCCGTTCCTGTCGTCACATCACATCTTCATTTCTCTGCAGGTTGGTGTCCCCGTTGACCGAATTAAAAGGTTGGGTGAAGAGGATAACTTTTGGACAAGCGGAGTCACCGGTCCCTGCGGTCCTTGCTCTGAAATTTATTATGATTTTCATCCTGAGAGGGGATACGCCGATGTTGTAAGTAGTATGATATACTTGTTACAAATTGAGTTATTTCAAATCACTTCACTGACTATGTTTTTTGGCTTGGGTGTTGAAAGCAGGATCTTGGTGATGATACACGGTTTATAGAGTTCTACAATTTAGTATTCATGCAATACAACAAAAAAGACGATGGTTCTCTCGAACCTCTAAAGCAGAAGAACATAGATACTGGGCTTGGTCTGGAGCGCATGGCTCGCATTCTTCAAAAGGTATTTCATTCTTTTGAATTGTTGTAAAAGTGAGTGAGTATTTGTACTTTTACAAGTGTTTCTAGGAGACTAGAACTAGAACTATGATATTTCTGCACCCTACACCACATCGTCGCTTAATGTTGAGAAAAGTGGGGCAAGGAAAAAGGGTGTGAATGAAACATTACTCACAAATCACAATACTCTCATTTTCATTTGCTCCTCAAGTCCTTTTTATATTTATTCATTATTCATTGCAGGCTCCAAATAACTATGAAACTGACTTGATTCTCCCCATCATAGAGAAGGCCTCTGAATTAGCAAATATTTCATATGGCATATCTGATGACCAGACAAAGAGGTATCTGAAAGTAAGTGTGACTATCTTCATACAAATTGTGGTAGAATTTTAAATAGTTTATTTCAATTCGCCTTATCACTTTTAATTTATGCTGTTTTTCCTTTACTTAGATTGTAGGAGATCACATGCGTGCAATTGTGTTTCTCATCTCTGATGGCGTTGTACCATCAAACGTTGGGAGGGGTTATGTAGTTCGACGGCTTATCAGAAGAGTTGTTCGTACAGGCAGGTTGCTTGGTATAAAAGGTGATGGTAGGGGAGACCCTGAAGGAGCATTTTTACCTATAATTGCTCAGAAAGTATTAGAGTTAAGCACTCACATTGATGCTGATGTGAAGAATAGAGCACCCCGTATCCTTGAGGAGTTGAAGAGAGAAGAGCTTCGATTTGTGCAGACGCTGGAGAGAGGAGAAAAATTGCTTGAGGAGAAGCTTGTTGATGCTATATCAAGTGCTGAAAGAAGTGGAACTGTACCTTGCTTGGCTGGTGAAGATGTGTTTATTTTGTATGATACTTATGGTTTCCCTATGGAAATAACAAAAGAAGTGGCTGAAGAACGCGGTGTGAGTATTGACATGAATGGTTTTGATATTGAGATGGAGAAGCAAAGACGTCAATCTCAAGCTGCACACAATACTGTCAAGCTTGCCATTGGAAGCGGGACAAATATTGCAGACAATGTACCTGACACTGAATTTATTGGATATGAAAGTCTTTATTGTAAAGCAATTGTAGCCAGCCTAGTGGTAAATGGTGATCCAGCCGTACAGGTTAGGGAAGGGAGTAATGTGGAAATTTTGCTGAACAAGACTCCATTTTATGCTGAATCAGGAGGACAAATTGGAGATCATGGTTTTCTGTATATATCAGAGGGTGAGAATCAACCTAAGGCTGTCGTGGAGATAATAGATGTTCAGAAGTCTTTTGGCAATATATTTGTTCACAAGGGTACAGTCCAAAAGGGTGTTCTAGAGGTTGGCAAACAAGTGGAAGCAACAGTGGATGTAAAGTTGAGGCAACGAGCCAAGGTACAAAGACTGTGTACCCACTCATTTTATTTATTTACTTCTTTGGTGAATCTTAAGTCATAAATAACAGAATTTGATACGGACAAAAATTTACATCATCATAGTTATGCCTATTTCCAAATCCGAAGCTTACAAGTTGTTCAAGATTACAGTAAATATAATTGGTGCTTTGCAGGTTCATCATACCGCTACTCATTTACTACAAGCTGCGCTTAAAAAGGTCATCGGTCAGGAGACCTCTCAAGCTGGTTCTTTGGTGGCATTTGATCGTCTCAGGTTTGATTTCAATTTCCACCGTCCACTCCTTGATAGCGAGCTTGCAGAAATCGAACTGCTGATCAACGGATGGATTGAGGATGCGATTCTTCTTCAGACCAAAGTGATGCCTCTTGCTGATGCAAAAAGAGAAGGAGCAATTGCAATGTTTGGAGAAAAATACGGGGAAGAGGTTGGACTTGCTACAAACGCTTTTGATATTTAACTTTGAAATTAATTTAGCTTACTTCTGTTTTGGTAGTTTTAAACATGAAGTGTGTACATAAACGAGCTCAGAAATTTGACCTTCAGGGATGGATTGCAACTAATGTTCAGGAATCATCATGTCTTCTGACCTAAATTCCCACATTACTATTACTATTGCCAGATTCCAATTACATTGTGATTTCAGATGCATCAATAGTTATCCCTCTCTCTCTATCACTTGTACCAGCACCTACACATGGACCATAGTAGTGAAATATACGGCATAGTAATATACTAGATTCTTAAAAAAAATTAAGAGCATCCAGCTGTGAAGAATTCTTAAACTGTACAAACGAAATGTAATCCCTAACAGCCCACAACCATGTCTACATCTAAGTTTGGTTATGACTTTGATTGGCAAAATTTTATTATTAGTGGCAGCTAGTGCCTAGTTGTTAGCAGCTTGGCTTATAGCTAAAAGTGGCCATGCTAACTGTCTGATGTTAAAGTATCTAATAAAGTTAGTGGTAAACTCGCTGATAAATATAAAATGACATGTCTATCTTTTTTTAGATAAATTGTGTTTATGTGTAGTATTGATTTGTAAATTATTATCGCTTTTAATACTTAAATTTATTTAATATACTTCTCATTTCTACATAAAATGCGATAATGCTAACAAAATGACATGTACACATAATTCTTTCATATAAATTTTAAGATTTTACAGGAAAATATATACTCTCCAGGGGCTAGGATTCTTTTGTTTTAAAGGGAGTATAATTAATATATTGAGAGTAAAAAGAAAAAAGAAAATAATACAATACAGACTATAGGCTCAGATGCTATATGAAATATAACACAAAAATAACCCTATAAAGTAGTGGGATAAGTTTCTTTATCAAACAACTCTAGTTATTAAATCCACTATAAACCAGTCAGGTCCACCGTAAGCTACTGGATCAATGTTGCCAATGAGATTATATATGTTCCATATTTTAGACATTTATATTTCTACTTCTCAATAATCGGCGAAAATAACCTGTTACTTCTCAATAACCTGTTTCTTCTTGTGTAATCATATTTGGTGTACTGCAGGTACGTGTTGTAGAGGTTCCTGGAGTATCGATGGAACTTTGTGGTGGGACTCATGTCAGTAATACTTCTGAAATTCGTGGTTTTAAAGTAATTTCAGAGCAGGGTATTGCATCTGGTGTCAGGCGAATAGAAGCTGTAGCTGGGGAAGCTTTCATTGAATATGTCAATGCTCGAGATTTATATCTGAAACAGCTATGTTCCACTCTCAAAGTATGTTCCTGATTTCAGTGTTTCAGAATTTTGTGCAAATAGTATAAATTTGTACAGAGTAGTTAATATCCATGACCACGAGTCATGCAATTGATATGACCAATACTTAATAATATAAATTTGTGCGCATAGGTATCTGTTTGTTTTAAGGGGGACCCTTGGCACAACGGTAAAGTTGTTGTCATGTGACTAAAAGGTCACCGGTTCAAGTCCTATAAACAGCCTAAAAGGGCAACCCGGTGCACTGAAGCTCCCGCATATGCAGGGTCCGGGAAGGGGTCCCACCATTTGGTGTATTGTACGCAGCCTTACCCTGTTTTTACACAAGAGGCTGTTTCCAGGACTTGAACCCGTGACCTTACGGTCACATGACAACAACTTTACCAGTTGCGCCAAGGTTACCCCTCAAGTCCTATAAACAGCCTCTTGTGTAAAAAACAGGTTAAGGCTGCGTATAATACACCAAAATGGCGGGACCCCTTCTCGGACCCTGCGTATGTGGGAGCTTTTTTGCACCGGGCTGCCTTTTATAGGTATCTGTTTGTTCTCCTTGCCCTTTGTTGGGGTTGAGTACACAGAAGAATTAGGGTTGAGAGACAAACCATTATATTTTTTACAAGATGGGATTCAATCACAATATGGGTAGGGTTTTCCATGCGGGTGTGGCTCAGTTGGTTATGTAGCATTGAGTTTATGGGGGAGGACCAAGATTCGATCCCCGCAGAACACACTATTGGGGAGGGGTGAAGAGCTTTCAGCTGCAACCTAGGCACTCGAGCGGAGATTAGTCCCATTGCTTGTCCAAAGGACTGAAGTCTGCTGTGATACTCTGTGGAGCCACTGAGGTGTCAAAGGCCCTAATTACAGCGTTTTCTTACCAAAGCCAAAATATTTACTACGACATTGCCATGCCTTACAAAGATTTTGTAGTTTGTTTCCTTGTGTATTATACATATAAGATAAGGGAACCGTCTCACTTTTTTCATGCTCGCACATTTGGGGTTCCTTGTTGCATGTGTTGCTCAAATACATAGAGTAGTATTTCCTTTATGTCTATAATAATCTTTAACATGAATTTTTAATAAAAAAGGGCAGCCCAGTGCACTAAAGCTCCCACATACGCAGGGTCCGGGAAAGGGGTCCTACCATTTTGGTGTATTGTAGATTTTGACGTTTTCTGCATCTGGTACCATTTCTATCCTTGAATAACTTATAATCAACTTTTTTGAAGGTTAAACCTGAAGAAGTAACTGTGAGGATAGAAAACCTTTTAGAGGAGTTACGGGTAGCAAGAAACGAAAATTCGGCTGTGCGTGCAAAAGCAGCAGTCTACAAAGCTTCAGTTATTGCAAGCAGAGCGTTTTTGGTGGGGCATTCAAAACAGTACAGGTAATACTTCTCCATCTTGTAGTATTTGTTACTTTAATGTGGGTTTGGGAGAGAACCTTCTCCTTGTTAGCAACACCAAAAAAAGTTTTTCTGATTAGATAATATATGCCTGTAAGGTACAAACTATTGTATATAACTGGGGAAGAAGGTGAAAAATATTTCCTTGATATAATGTCATGTCATAACCAACTGAAATCCAACAATTGGAAACTGATCCAGATATCTATGAAAAGGGAGCCCAAAATAAGTTTAACAAATAAATATTAAATAAACAATTAATTATAACAGTATAATACAATTAAAAAACTAATAATAAATTTTTAATGAACTCCATACATACTCAAAATATAGCAGCTCGAAACTCTAAATTCGTTAGGGAAGTGTGCAACGGAGGAAGGCTTAATGGGTGTTCTATTTTTTTCCCAATAGCACTTATATTATAAGTGCTGTGGTACTCTATTTCTAAAGTTAAAAAAGAGTTCATGTAATTAGGAGGAAAAAAATGGAAGTTGATTAATTTAACTCCCATGCAAAAGGCTAGTTTTGAATAAAATTAATAGATGCCAAAAGCTTGGATGAATTGAGCTAATGTATATTGTTATTCATACTCAAGCATATAAATCTGCATTCACTTCGTCAATTTGATTTTCTTCTAATTAGGATTATTCAAGAGATTGGCGTTGATAATTGTTAAAACAATAAAACCTAAAAATTAATTTATTCCTAATTAAATTAACCATTCAAAGGTAGCCCAATAACTGCTAAAACAAAAGTTGTCTGAGCAGGGACAGATTGGAAAGGGAAATGCACGGCCCCTTCCATCTCCCCTTTTCTCGTTTGCAGATACAAACCTCCTGGGTGAAAGGCGATATTCCTATTCTAGCAGCGGAATTTCTGCTTCAGATATAAAAGTCTAACTTTTCACACCCGGCTTGTGCGCTTTTGCTTTAGGAAACTCCACCAACGACAAAATCTTTATCAACTATTTAGAGGAGCACAAGCTTTTCACCTTTACCAACTCAGTAAAGCTCCAAAGTTCATCTGTTTCAACGGATGGCCCCCTAAATATTATTTAACTATTATAAATTAATATTCATGTGATATTTATAATCATATATTTTTTGACCTCTCATATGATATAGTTTTGGATCTGTCCGTGTTTGAGTGCAAACTATTCTGATGGTTATCCATATAACTTTAGTAATACCGACTGGAATGATATGCATTGATATGCCATGTCATAGAAGTAGAATGACATGGGACAAGGGTAGGTTACACACATGCGTGTCCCCCCTCCCCTGGGTTCGTCTCTGCCGGAAAAATAGAAGCAGAGGTCTTGCACATCATTGATGTTCCCTCGAATATATTTTCTGTGAATGCTGCTGGCATAACTAGAACATGGGAGGATGTCTTTCATATGTGTATTTCCACGAAAGGGTGACTTGGTGCTAAAACTCCCGCACCCTTGTGGGTTTCAGGATAGAGTCAGACCCATTGTGAGTTTGATAAAGGGCAACCTAATCGTAGTGTGTTATAAAGCTCCCGCGGTGGGCTACGGGGGCTTGCACACGCACAGGTCTGTTGCAGGTAATCTTTCCTGGCACCTAACCTTTGACCTCGTAATCAACACTACAATAACTCTAACTATTGTACACAAACTTTCCTTCATTGTGCGTCTACTGTATATACAGGACTGGAAATAGAAAAGAAAAATTATGGTTGAAAATGTAATGATTGTATGGTCTTTAAGTTGATGCATCATTTTTATTTATTAATTATTGAAAGGGAAATGTTAACCAGTGCCCCCGGGGCACTGGTTAAGGCCTATAAAAGGTAACTTATCATGAAAATACGTGTAATCAATGTATCGGGAATTGAAATATTTGACTTTTTTGTATAATAATTTCTTTTTTTGGAATCCTTAAAGAGTGCCCCGGGGGCACTCGTTAACAAGACCCTTATTGAAAATCTTGCAGGATACTAGTTGAGTGTTTGGATGATGTTGATGCGGAGTCACTTAAAAGTGCCGCAGAATATTTAATGGAAACATTAGCAGATCCAGCAGCTATTGTCCTGGGCTCATGTCCAGGTGAAGGGAAGGTTAGTCTAGTGGCTGCTTTTACTCCAGGGGTGGTTGGTCAGGGAATTCAGGCAGGTAAATTTATTGGACAGATAGCAAAATTATGTGGTGGTGGAGGAGGTGGAAGGTCCAACTTCGCTCAGGCTGGTGGGAGGAAGCCTGAAAATTTGGCTGGTGCCCTCGAGAAAGCTAAATCAGAGCTTATAGCTTATCTTTGTGAAAAAGGGAACTGA
Protein sequence

>MS.gene68122.t1

MNVTLAVAAGAGGFISFKQLHLRRSRRLLPFCTSSSQDNDNNNNNNNNNNNNNNCVSGDSIRERFLSFYASRGHKVLPSASLVPDDPTVLLTIAGMLQFKPIFLGKLPRQVPRATTSQRCIRTNDINNVGHTSRHHTFFEMLGNFSFGDYFKKQAIQWAWDLSTIEFGLPPERLWISVYQDDHETFQLWSQQVGVPVDRIKRLGEEDNFWTSGVTGPCGPCSEIYYDFHPERGYADVDLGDDTRFIEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQKAPNNYETDLILPIIEKASELANISYGISDDQTKRYLKIVGDHMRAIVFLISDGVVPSNVGRGYVVRRLIRRVVRTGRLLGIKGDGRGDPEGAFLPIIAQKVLELSTHIDADVKNRAPRILEELKREELRFVQTLERGEKLLEEKLVDAISSAERSGTVPCLAGEDVFILYDTYGFPMEITKEVAEERGVSIDMNGFDIEMEKQRRQSQAAHNTVKLAIGSGTNIADNVPDTEFIGYESLYCKAIVASLVVNGDPAVQVREGSNVEILLNKTPFYAESGGQIGDHGFLYISEGENQPKAVVEIIDVQKSFGNIFVHKGTVQKGVLEVGKQVEATVDVKLRQRAKVHHTATHLLQAALKKVIGQETSQAGSLVAFDRLRFDFNFHRPLLDSELAEIELLINGWIEDAILLQTKVMPLADAKREGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVSNTSEIRGFKVISEQGIASGVRRIEAVAGEAFIEYVNARDLYLKQLCSTLKVKPEEVTVRIENLLEELRVARNENSAVRAKAAVYKASVIASRAFLVGHSKQYRILVECLDDVDAESLKSAAEYLMETLADPAAIVLGSCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRSNFAQAGGRKPENLAGALEKAKSELIAYLCEKGN