AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84154


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84154.t1 MTR_2g036690 98.522 203 3 0 1 203 40 242 1.18e-144 402
MS.gene84154.t1 MTR_2g036690 98.582 141 2 0 1 141 40 180 2.92e-98 283
MS.gene84154.t1 MTR_2g005900 71.341 164 47 0 39 202 60 223 1.83e-78 234
MS.gene84154.t1 MTR_2g005900 39.130 92 51 2 113 200 53 143 4.99e-11 60.1
MS.gene84154.t1 MTR_7g109850 37.888 161 98 2 32 191 49 208 9.98e-25 96.7
MS.gene84154.t1 MTR_8g038785 44.318 88 49 0 31 118 48 135 2.77e-16 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84154.t1 AT4G25910 69.608 204 59 2 1 203 35 236 7.85e-94 273
MS.gene84154.t1 AT5G49940 70.225 178 49 1 25 202 62 235 2.84e-81 242
MS.gene84154.t1 AT5G49940 65.812 117 36 1 25 141 62 174 1.72e-44 146
MS.gene84154.t1 AT5G49940 45.946 74 35 2 133 202 85 157 1.15e-11 61.2
MS.gene84154.t1 AT4G01940 36.649 191 115 5 2 191 38 223 2.72e-25 98.6

Find 35 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAACCTGCTTTGTTTCT+TGG 0.155090 2.3:-52020423 None:intergenic
TGTTCAACCTCCAAGATTTC+TGG 0.161640 2.3:-52021116 None:intergenic
GAAATTAGACCATATCTTAT+TGG 0.195631 2.3:+52021549 MS.gene84154:CDS
AGACCATATCTTATTGGAAC+TGG 0.334639 2.3:+52021555 MS.gene84154:CDS
AGAAAATGTAGAGAAAGTTT+TGG 0.348094 2.3:+52020932 MS.gene84154:CDS
CACAAGCTCCTTGTAGTTTG+AGG 0.369342 2.3:-52021028 None:intergenic
CGATAAGATTCCAGAAATCT+TGG 0.378676 2.3:+52021106 MS.gene84154:CDS
ATGATTACATCATTAAAGTT+CGG 0.426909 2.3:+52021607 MS.gene84154:CDS
CGGCTAAGTGGACCGGCAGC+AGG 0.440486 2.3:+52021627 MS.gene84154:CDS
AGCTGCACAGCAGCAATAGA+AGG 0.442231 2.3:-52021699 None:intergenic
TGAAGTAAGGCCTGGTTTGA+TGG 0.459751 2.3:+52020956 MS.gene84154:CDS
ATCATTAAAGTTCGGCTAAG+TGG 0.471253 2.3:+52021615 MS.gene84154:CDS
CTCAACCATGACATTGAAGA+TGG 0.471530 2.3:+52021064 MS.gene84154:CDS
TTCTTGAAGAATCTTATAGC+AGG 0.514284 2.3:+52020309 None:intergenic
CTCAAACTACAAGGAGCTTG+TGG 0.520248 2.3:+52021029 MS.gene84154:CDS
GACCATATCTTATTGGAACT+GGG 0.523059 2.3:+52021556 MS.gene84154:CDS
GATCCCCATCTTCAATGTCA+TGG 0.540108 2.3:-52021069 None:intergenic
CCTCCATCAGCCATCAAACC+AGG 0.556402 2.3:-52020966 None:intergenic
GTTGTTATCCTCAAACTACA+AGG 0.559144 2.3:+52021020 MS.gene84154:CDS
TAAAGTTCGGCTAAGTGGAC+CGG 0.560824 2.3:+52021620 MS.gene84154:CDS
GTGGCATTGCATGAGATAGA+TGG 0.565280 2.3:+52020993 MS.gene84154:CDS
TTCAATGTCATGGTTGAGCT+AGG 0.567639 2.3:-52021059 None:intergenic
TCAACCATGACATTGAAGAT+GGG 0.574515 2.3:+52021065 MS.gene84154:CDS
CAGATACTAGATACTGAGAC+TGG 0.580123 2.3:+52021137 MS.gene84154:CDS
TAAGATTCCAGAAATCTTGG+AGG 0.581548 2.3:+52021109 MS.gene84154:CDS
CTGGTTTGATGGCTGATGGA+GGG 0.590897 2.3:+52020967 MS.gene84154:CDS
CCTGGTTTGATGGCTGATGG+AGG 0.592266 2.3:+52020966 MS.gene84154:CDS
GTCAGTGGCAGGACACATCT+CGG 0.597217 2.3:-52020906 None:intergenic
ACCATATCTTATTGGAACTG+GGG 0.604069 2.3:+52021557 MS.gene84154:CDS
CCATATCTTATTGGAACTGG+GGG 0.604476 2.3:+52021558 MS.gene84154:CDS
GATGGCTGATGGAGGGAATG+TGG 0.613469 2.3:+52020974 MS.gene84154:CDS
AGGCCTGGTTTGATGGCTGA+TGG 0.614038 2.3:+52020963 MS.gene84154:CDS
CGTGCTCCAAGAAACAAAGC+AGG 0.615747 2.3:+52020417 MS.gene84154:CDS
CAACCATGACATTGAAGATG+GGG 0.616574 2.3:+52021066 MS.gene84154:CDS
ACTGTCATAACTCCTGCTGC+CGG 0.622897 2.3:-52021639 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAAATTATTCATTTTTTC+AGG + chr2.3:52021518-52021537 MS.gene84154:intron 10.0%
!! AAAATCATGAATTCTTATTA+AGG - chr2.3:52020604-52020623 None:intergenic 15.0%
!!! ATTAATATTAAGCAAGTTTT+CGG + chr2.3:52020381-52020400 MS.gene84154:CDS 15.0%
!!! GTTAAATTTTAATCAATTCT+TGG + chr2.3:52020486-52020505 MS.gene84154:intron 15.0%
!! ATGATTACATCATTAAAGTT+CGG + chr2.3:52021607-52021626 MS.gene84154:CDS 20.0%
!! GTGATATACAAATGAAATTA+TGG - chr2.3:52021232-52021251 None:intergenic 20.0%
!!! AATCAATTCTTGGATTTTAT+TGG + chr2.3:52020496-52020515 MS.gene84154:intron 20.0%
!!! TTAATATTAAGCAAGTTTTC+GGG + chr2.3:52020382-52020401 MS.gene84154:CDS 20.0%
! AATTTCTCTACAAACATGTA+AGG - chr2.3:52021401-52021420 None:intergenic 25.0%
! GAAATTAGACCATATCTTAT+TGG + chr2.3:52021549-52021568 MS.gene84154:CDS 25.0%
!! AGAAAATGTAGAGAAAGTTT+TGG + chr2.3:52020932-52020951 MS.gene84154:CDS 25.0%
ACTAAGTTGTTTATCCAAAC+AGG + chr2.3:52021341-52021360 MS.gene84154:intron 30.0%
ATGCTTCATAGATTGCAATT+TGG - chr2.3:52020628-52020647 None:intergenic 30.0%
! ACGCTATTCTTGAGATTTTA+TGG - chr2.3:52021286-52021305 None:intergenic 30.0%
! CGCTATTCTTGAGATTTTAT+GGG - chr2.3:52021285-52021304 None:intergenic 30.0%
! TACACTTTCGACATTTTCTT+CGG - chr2.3:52021174-52021193 None:intergenic 30.0%
!! GCTTTTATGTTTTGTTACGA+AGG - chr2.3:52021257-52021276 None:intergenic 30.0%
!!! AGAAAGTTTTGGATGAAGTA+AGG + chr2.3:52020943-52020962 MS.gene84154:CDS 30.0%
!!! CATTGCAATGTTTTTTTTGC+AGG + chr2.3:52020870-52020889 MS.gene84154:intron 30.0%
ACCATATCTTATTGGAACTG+GGG + chr2.3:52021557-52021576 MS.gene84154:CDS 35.0%
AGACCATATCTTATTGGAAC+TGG + chr2.3:52021555-52021574 MS.gene84154:CDS 35.0%
ATCATTAAAGTTCGGCTAAG+TGG + chr2.3:52021615-52021634 MS.gene84154:CDS 35.0%
CGATAAGATTCCAGAAATCT+TGG + chr2.3:52021106-52021125 MS.gene84154:CDS 35.0%
GAAAACAGTTTGACCTGATA+CGG + chr2.3:52021308-52021327 MS.gene84154:intron 35.0%
GACCATATCTTATTGGAACT+GGG + chr2.3:52021556-52021575 MS.gene84154:CDS 35.0%
GTTGTTATCCTCAAACTACA+AGG + chr2.3:52021020-52021039 MS.gene84154:CDS 35.0%
TAAGATTCCAGAAATCTTGG+AGG + chr2.3:52021109-52021128 MS.gene84154:CDS 35.0%
TCAACCATGACATTGAAGAT+GGG + chr2.3:52021065-52021084 MS.gene84154:CDS 35.0%
TTGTGAACTTAGATCCTGTT+TGG - chr2.3:52021358-52021377 None:intergenic 35.0%
! ATCTAATCAAGTAGCTTGCA+AGG + chr2.3:52020816-52020835 MS.gene84154:intron 35.0%
! ATGTCGAAAGTGTACGTAAT+TGG + chr2.3:52021180-52021199 MS.gene84154:intron 35.0%
! TCTAATCAAGTAGCTTGCAA+GGG + chr2.3:52020817-52020836 MS.gene84154:intron 35.0%
! TCTACATTTTCTTCTGTCAG+TGG - chr2.3:52020924-52020943 None:intergenic 35.0%
!! AATGAGAGTTGTTTTCTCAG+AGG + chr2.3:52020351-52020370 MS.gene84154:CDS 35.0%
AGTTAAGCACTTACCGTATC+AGG - chr2.3:52021324-52021343 None:intergenic 40.0%
CAACCATGACATTGAAGATG+GGG + chr2.3:52021066-52021085 MS.gene84154:CDS 40.0%
CAGATACTAGATACTGAGAC+TGG + chr2.3:52021137-52021156 MS.gene84154:CDS 40.0%
CCATATCTTATTGGAACTGG+GGG + chr2.3:52021558-52021577 MS.gene84154:CDS 40.0%
CTCAACCATGACATTGAAGA+TGG + chr2.3:52021064-52021083 MS.gene84154:CDS 40.0%
TGTTCAACCTCCAAGATTTC+TGG - chr2.3:52021119-52021138 None:intergenic 40.0%
TTCAATGTCATGGTTGAGCT+AGG - chr2.3:52021062-52021081 None:intergenic 40.0%
!! GAAGAACCTGCTTTGTTTCT+TGG - chr2.3:52020426-52020445 None:intergenic 40.0%
CACAAGCTCCTTGTAGTTTG+AGG - chr2.3:52021031-52021050 None:intergenic 45.0%
CCAAACACGTCGTCAATCTA+AGG + chr2.3:52020768-52020787 MS.gene84154:intron 45.0%
CCCCCAGTTCCAATAAGATA+TGG - chr2.3:52021561-52021580 None:intergenic 45.0%
CCTTAGATTGACGACGTGTT+TGG - chr2.3:52020771-52020790 None:intergenic 45.0%
CTCAAACTACAAGGAGCTTG+TGG + chr2.3:52021029-52021048 MS.gene84154:CDS 45.0%
GATCCCCATCTTCAATGTCA+TGG - chr2.3:52021072-52021091 None:intergenic 45.0%
GTAACGTGTCAGTGTCATGT+TGG + chr2.3:52020719-52020738 MS.gene84154:intron 45.0%
GTGGCATTGCATGAGATAGA+TGG + chr2.3:52020993-52021012 MS.gene84154:CDS 45.0%
TAAAGTTCGGCTAAGTGGAC+CGG + chr2.3:52021620-52021639 MS.gene84154:CDS 45.0%
TATCTTATTGGAACTGGGGG+AGG + chr2.3:52021561-52021580 MS.gene84154:CDS 45.0%
! CATTTTCTTCTGTCAGTGGC+AGG - chr2.3:52020920-52020939 None:intergenic 45.0%
! TGAAGTAAGGCCTGGTTTGA+TGG + chr2.3:52020956-52020975 MS.gene84154:CDS 45.0%
! TTGGTGTCTGACATGTGTCA+GGG - chr2.3:52020752-52020771 None:intergenic 45.0%
!! GTTTTGGATGAAGTAAGGCC+TGG + chr2.3:52020948-52020967 MS.gene84154:CDS 45.0%
!! TTTGGTGTCTGACATGTGTC+AGG - chr2.3:52020753-52020772 None:intergenic 45.0%
ACTGTCATAACTCCTGCTGC+CGG - chr2.3:52021642-52021661 None:intergenic 50.0%
AGCTGCACAGCAGCAATAGA+AGG - chr2.3:52021702-52021721 None:intergenic 50.0%
CGTGCTCCAAGAAACAAAGC+AGG + chr2.3:52020417-52020436 MS.gene84154:CDS 50.0%
! CTGGTTTGATGGCTGATGGA+GGG + chr2.3:52020967-52020986 MS.gene84154:CDS 50.0%
CCTCCATCAGCCATCAAACC+AGG - chr2.3:52020969-52020988 None:intergenic 55.0%
GATGGCTGATGGAGGGAATG+TGG + chr2.3:52020974-52020993 MS.gene84154:CDS 55.0%
GTCAGTGGCAGGACACATCT+CGG - chr2.3:52020909-52020928 None:intergenic 55.0%
! AGGCCTGGTTTGATGGCTGA+TGG + chr2.3:52020963-52020982 MS.gene84154:CDS 55.0%
! CCTGGTTTGATGGCTGATGG+AGG + chr2.3:52020966-52020985 MS.gene84154:CDS 55.0%
CGGCTAAGTGGACCGGCAGC+AGG + chr2.3:52021627-52021646 MS.gene84154:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 52020318 52021731 52020318 ID=MS.gene84154
chr2.3 mRNA 52020318 52021731 52020318 ID=MS.gene84154.t1;Parent=MS.gene84154
chr2.3 exon 52020318 52020438 52020318 ID=MS.gene84154.t1.exon1;Parent=MS.gene84154.t1
chr2.3 CDS 52020318 52020438 52020318 ID=cds.MS.gene84154.t1;Parent=MS.gene84154.t1
chr2.3 exon 52020892 52021190 52020892 ID=MS.gene84154.t1.exon2;Parent=MS.gene84154.t1
chr2.3 CDS 52020892 52021190 52020892 ID=cds.MS.gene84154.t1;Parent=MS.gene84154.t1
chr2.3 exon 52021540 52021731 52021540 ID=MS.gene84154.t1.exon3;Parent=MS.gene84154.t1
chr2.3 CDS 52021540 52021731 52021540 ID=cds.MS.gene84154.t1;Parent=MS.gene84154.t1
Gene Sequence

>MS.gene84154

AATCTTATAGCAGGTAATAGAAGATTTTCATCAAATGAGAGTTGTTTTCTCAGAGGTCATCAAATTAATATTAAGCAAGTTTTCGGGCTAATCTCTCGTCGTGCTCCAAGAAACAAAGCAGGTTCTTCTAGAGTTTCATCAATTTGAATATACTTGTATTGATTATTTGTTAAATTTTAATCAATTCTTGGATTTTATTGGATGAAAGTTAGTACTAGTAGTTTAGTATAATACATTTCAATTGTTTAGCACTAAGAGAATTGAAAGTGTGAGTGATTTTGTTCCTTAATAAGAATTCATGATTTTACCAAATTGCAATCTATGAAGCATGATACAGACACACGACACTCATATTGACACGTAGCTTTCGTTAATAATTTAAAAAATACAAGTGATTATACGTAACGTGTCAGTGTCATGTTGGTGTCAGACCCTGACACATGTCAGACACCAAACACGTCGTCAATCTAAGGTGATAGTGCTACATAGATTGCAATCATCTAATCAAGTAGCTTGCAAGGGATGTGAGAAAATAGTTGAATCTATCATTCACATTGCAATGTTTTTTTTGCAGGACTTGTTGTTTCTCCGAGATGTGTCCTGCCACTGACAGAAGAAAATGTAGAGAAAGTTTTGGATGAAGTAAGGCCTGGTTTGATGGCTGATGGAGGGAATGTGGCATTGCATGAGATAGATGGTCTTGTTGTTATCCTCAAACTACAAGGAGCTTGTGGATCATGTCCTAGCTCAACCATGACATTGAAGATGGGGATCGAAACTCGTCTTCGCGATAAGATTCCAGAAATCTTGGAGGTTGAACAGATACTAGATACTGAGACTGGTCTTGAGCTAACCGAAGAAAATGTCGAAAGTGTACGTAATTGGAAACTTATGCATCATTTCTTGCATTGCCATAATTTCATTTGTATATCACTTCCTTCGTAACAAAACATAAAAGCTTCTTCCCATAAAATCTCAAGAATAGCGTATGAAAACAGTTTGACCTGATACGGTAAGTGCTTAACTAAGTTGTTTATCCAAACAGGATCTAAGTTCACAAATATTCTCATATTTATAGTGCCTTACATGTTTGTAGAGAAATTATCTATAAGAAAACATTAACATGTATCTATATAAGCTGCATAATTGATGTTGCTAGTGACAACTTCTTGTTGCTTTAATGTAAAGATTGCGAATCATATAAAATTATTCATTTTTTCAGGTTCTTTCAGAAATTAGACCATATCTTATTGGAACTGGGGGAGGAACATTAGAGCTTGTTGAGATTAATGATTACATCATTAAAGTTCGGCTAAGTGGACCGGCAGCAGGAGTTATGACAGTTCGTGTTGCGTTAACACAGAAGCTGAGAGACAAGATACCTTCTATTGCTGCTGTGCAGCTAATAGAATGA

Protein sequence

>MS.gene84154.t1

NLIAGNRRFSSNESCFLRGHQINIKQVFGLISRRAPRNKAGLVVSPRCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSCPSSTMTLKMGIETRLRDKIPEILEVEQILDTETGLELTEENVESVLSEIRPYLIGTGGGTLELVEINDYIIKVRLSGPAAGVMTVRVALTQKLRDKIPSIAAVQLIE