AlfalfaGEDB | MS.gene06476

M. sativa cultivar XinJiangDaYe / MS.gene06476

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06476.t1	PNY08358.1	77.2	219	50	0	1	219	126	344	3.80E-88	334.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06476.t1	Q9FJT1	26.2	309	169	9	6	261	126	428	3.8e-12	73.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06476.t1	A0A2K3NZ97	77.2	219	50	0	1	219	126	344	2.7e-88	334.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene06476	MS.gene34733	PPI
MS.gene06476	MS.gene99983	PPI
MS.gene34739	MS.gene06476	PPI
MS.gene022915	MS.gene06476	PPI
MS.gene36764	MS.gene06476	PPI
MS.gene36942	MS.gene06476	PPI
MS.gene97355	MS.gene06476	PPI
MS.gene065516	MS.gene06476	PPI
MS.gene014840	MS.gene06476	PPI
MS.gene47780	MS.gene06476	PPI
MS.gene34753	MS.gene06476	PPI
MS.gene06476	MS.gene032379	PPI
MS.gene06476	MS.gene043122	PPI
MS.gene61631	MS.gene06476	PPI
MS.gene06476	MS.gene48654	PPI
MS.gene39752	MS.gene06476	PPI
MS.gene022909	MS.gene06476	PPI
MS.gene36763	MS.gene06476	PPI
MS.gene36804	MS.gene06476	PPI
MS.gene06476	MS.gene98323	PPI
MS.gene06476	MS.gene92095	PPI
MS.gene47782	MS.gene06476	PPI
MS.gene39745	MS.gene06476	PPI
MS.gene055754	MS.gene06476	PPI
MS.gene06476	MS.gene022912	PPI
MS.gene06476	MS.gene49018	PPI
MS.gene06476	MS.gene007875	PPI
MS.gene47775	MS.gene06476	PPI
MS.gene06476	MS.gene062282	PPI
MS.gene06476	MS.gene062526	PPI
MS.gene72957	MS.gene06476	PPI
MS.gene36752	MS.gene06476	PPI
MS.gene032379	MS.gene06476	PPI
MS.gene06476	MS.gene010866	PPI
MS.gene32300	MS.gene06476	PPI
MS.gene06476	MS.gene63224	PPI
MS.gene63210	MS.gene06476	PPI
MS.gene06476	MS.gene34736	PPI
MS.gene06476	MS.gene25414	PPI
MS.gene63217	MS.gene06476	PPI
MS.gene06476	MS.gene49013	PPI
MS.gene050239	MS.gene06476	PPI
MS.gene06476	MS.gene34730	PPI
MS.gene06476	MS.gene065374	PPI
MS.gene30067	MS.gene06476	PPI
MS.gene06476	MS.gene25418	PPI
MS.gene47777	MS.gene06476	PPI
MS.gene32643	MS.gene06476	PPI
MS.gene36067	MS.gene06476	PPI
MS.gene86337	MS.gene06476	PPI
MS.gene06476	MS.gene072885	PPI
MS.gene014842	MS.gene06476	PPI
MS.gene06476	MS.gene49011	PPI
MS.gene06476	MS.gene25296	PPI
MS.gene065234	MS.gene06476	PPI
MS.gene06476	MS.gene72962	PPI
MS.gene39749	MS.gene06476	PPI
MS.gene053313	MS.gene06476	PPI
MS.gene06476	MS.gene073176	PPI
MS.gene06476	MS.gene72952	PPI
MS.gene25396	MS.gene06476	PPI
MS.gene23706	MS.gene06476	PPI
MS.gene06476	MS.gene89076	PPI
MS.gene49018	MS.gene06476	PPI
MS.gene06476	MS.gene060769	PPI
MS.gene06476	MS.gene39747	PPI
MS.gene06476	MS.gene022919	PPI
MS.gene06476	MS.gene72956	PPI
MS.gene06476	MS.gene49012	PPI
MS.gene49513	MS.gene06476	PPI
MS.gene69850	MS.gene06476	PPI
MS.gene06476	MS.gene47771	PPI
MS.gene06476	MS.gene72961	PPI
MS.gene06476	MS.gene072231	PPI
MS.gene06476	MS.gene39753	PPI
MS.gene072244	MS.gene06476	PPI
MS.gene63214	MS.gene06476	PPI
MS.gene032296	MS.gene06476	PPI
MS.gene26058	MS.gene06476	PPI
MS.gene72963	MS.gene06476	PPI
MS.gene06476	MS.gene022917	PPI
MS.gene34735	MS.gene06476	PPI
MS.gene046839	MS.gene06476	PPI
MS.gene32638	MS.gene06476	PPI
MS.gene060771	MS.gene06476	PPI
MS.gene06476	MS.gene54958	PPI
MS.gene32305	MS.gene06476	PPI
MS.gene63095	MS.gene06476	PPI
MS.gene72955	MS.gene06476	PPI
MS.gene63104	MS.gene06476	PPI
MS.gene06476	MS.gene63218	PPI
MS.gene06476	MS.gene014841	PPI
MS.gene043123	MS.gene06476	PPI
MS.gene06476	MS.gene056517	PPI
MS.gene06476	MS.gene024263	PPI
MS.gene06476	MS.gene36065	PPI
MS.gene06476	MS.gene43257	PPI
MS.gene93027	MS.gene06476	PPI
MS.gene49017	MS.gene06476	PPI
MS.gene32641	MS.gene06476	PPI
MS.gene34215	MS.gene06476	PPI
MS.gene06476	MS.gene21682	PPI
MS.gene022905	MS.gene06476	PPI
MS.gene30260	MS.gene06476	PPI
MS.gene06476	MS.gene014842	PPI
MS.gene06476	MS.gene63185	PPI
MS.gene06476	MS.gene95710	PPI
MS.gene36810	MS.gene06476	PPI
MS.gene36743	MS.gene06476	PPI
MS.gene007794	MS.gene06476	PPI
MS.gene39744	MS.gene06476	PPI
MS.gene06476	MS.gene022921	PPI
MS.gene06476	MS.gene056133	PPI
MS.gene48656	MS.gene06476	PPI
MS.gene63223	MS.gene06476	PPI
MS.gene26069	MS.gene06476	PPI
MS.gene06476	MS.gene36809	PPI
MS.gene72959	MS.gene06476	PPI
MS.gene06476	MS.gene64984	PPI
MS.gene34731	MS.gene06476	PPI
MS.gene06476	MS.gene36943	PPI
MS.gene06476	MS.gene36807	PPI
MS.gene067702	MS.gene06476	PPI
MS.gene022916	MS.gene06476	PPI
MS.gene63212	MS.gene06476	PPI
MS.gene06476	MS.gene98324	PPI
MS.gene06476	MS.gene34737	PPI
MS.gene055880	MS.gene06476	PPI
MS.gene25412	MS.gene06476	PPI
MS.gene06476	MS.gene39754	PPI
MS.gene25410	MS.gene06476	PPI
MS.gene06476	MS.gene47772	PPI
MS.gene038427	MS.gene06476	PPI
MS.gene30045	MS.gene06476	PPI
MS.gene06476	MS.gene017193	PPI
MS.gene06476	MS.gene060770	PPI
MS.gene06476	MS.gene49015	PPI
MS.gene06476	MS.gene69407	PPI
MS.gene002108	MS.gene06476	PPI
MS.gene033696	MS.gene06476	PPI
MS.gene72960	MS.gene06476	PPI
MS.gene06476	MS.gene022920	PPI
MS.gene06476	MS.gene046838	PPI
MS.gene06476	MS.gene47781	PPI
MS.gene47783	MS.gene06476	PPI
MS.gene059405	MS.gene06476	PPI
MS.gene25411	MS.gene06476	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06476.t1	MTR_3g107120	60.078	258	101	1	5	262	139	394	2.58e-104	308
MS.gene06476.t1	MTR_3g107160	62.016	258	94	2	2	257	126	381	5.09e-102	302
MS.gene06476.t1	MTR_3g107100	52.015	273	112	3	1	256	125	395	7.49e-76	236
MS.gene06476.t1	MTR_3g106960	63.212	193	65	4	64	252	1	191	8.47e-70	215
MS.gene06476.t1	MTR_3g094980	93.396	106	7	0	141	246	26	131	4.05e-66	203
MS.gene06476.t1	MTR_1g107385	44.444	261	129	11	7	256	165	420	6.32e-51	172
MS.gene06476.t1	MTR_2g007400	43.191	257	131	8	11	256	148	400	1.39e-50	171
MS.gene06476.t1	MTR_2g007450	41.852	270	134	6	7	256	151	417	2.76e-50	170
MS.gene06476.t1	MTR_7g075820	43.629	259	129	8	7	256	141	391	5.35e-50	169
MS.gene06476.t1	MTR_2g008320	42.529	261	129	8	8	256	173	424	5.22e-48	165
MS.gene06476.t1	MTR_3g099830	41.445	263	133	9	7	256	117	371	3.97e-47	161
MS.gene06476.t1	MTR_2g008310	41.085	258	135	8	8	256	119	368	5.08e-47	161
MS.gene06476.t1	MTR_1g053025	39.474	266	144	8	7	259	128	389	8.66e-46	158
MS.gene06476.t1	MTR_2g005990	41.538	260	136	10	8	256	138	392	3.68e-45	156
MS.gene06476.t1	MTR_7g007080	40.927	259	144	7	5	259	126	379	3.19e-44	154
MS.gene06476.t1	MTR_5g083890	41.313	259	123	9	7	246	141	389	1.61e-43	154
MS.gene06476.t1	MTR_4g098590	40.891	247	130	6	7	243	126	366	2.01e-43	151
MS.gene06476.t1	MTR_2g007360	38.745	271	139	11	7	256	107	371	5.04e-43	150
MS.gene06476.t1	MTR_6g029350	45.854	205	96	8	57	256	1	195	4.83e-41	141
MS.gene06476.t1	MTR_7g007340	41.245	257	129	11	7	253	107	351	1.21e-40	144
MS.gene06476.t1	MTR_7g056280	39.336	211	122	3	58	262	367	577	4.22e-39	143
MS.gene06476.t1	MTR_3g026920	37.828	267	124	10	7	257	163	403	1.78e-37	136
MS.gene06476.t1	MTR_7g083740	37.600	250	138	7	21	262	131	370	1.83e-37	135
MS.gene06476.t1	MTR_2g007500	38.813	219	118	4	7	212	120	335	2.61e-35	129
MS.gene06476.t1	MTR_2g007550	40.201	199	98	6	73	257	142	333	9.69e-34	125
MS.gene06476.t1	MTR_7g075800	31.724	290	150	9	7	251	140	426	5.43e-32	124
MS.gene06476.t1	MTR_2g007090	37.398	246	124	12	20	259	73	294	6.54e-32	119
MS.gene06476.t1	MTR_3g108150	34.962	266	155	5	6	258	126	386	8.11e-31	118
MS.gene06476.t1	MTR_7g032380	32.331	266	129	8	6	258	128	355	1.76e-30	117
MS.gene06476.t1	MTR_1g041245	33.209	268	155	10	6	258	127	385	1.60e-29	114
MS.gene06476.t1	MTR_7g098570	35.039	254	148	7	6	251	135	379	3.21e-29	115
MS.gene06476.t1	MTR_7g098510	31.955	266	160	7	6	258	133	390	4.48e-29	113
MS.gene06476.t1	MTR_7g032370	33.708	267	142	9	6	258	106	351	2.75e-28	110
MS.gene06476.t1	MTR_7g115270	32.234	273	156	7	6	256	134	399	4.05e-28	111
MS.gene06476.t1	MTR_7g098480	32.171	258	152	7	6	253	133	377	4.07e-28	112
MS.gene06476.t1	MTR_7g033990	33.628	226	120	11	39	251	167	375	4.92e-28	111
MS.gene06476.t1	MTR_7g033915	32.510	243	133	11	29	258	140	364	2.61e-27	108
MS.gene06476.t1	MTR_7g034120	33.984	256	149	9	11	258	64	307	3.94e-27	107
MS.gene06476.t1	MTR_5g008950	32.824	262	159	6	6	258	111	364	1.23e-26	107
MS.gene06476.t1	MTR_5g007410	31.837	245	139	9	6	232	141	375	1.71e-26	107
MS.gene06476.t1	MTR_6g045333	30.855	269	156	8	11	258	186	445	1.78e-26	107
MS.gene06476.t1	MTR_7g056433	32.117	274	150	10	6	258	130	388	2.18e-26	106
MS.gene06476.t1	MTR_7g034090	40.936	171	89	5	96	259	147	312	5.43e-26	105
MS.gene06476.t1	MTR_7g026500	30.962	239	138	9	34	258	169	394	5.88e-26	105
MS.gene06476.t1	MTR_2g007610	42.336	137	75	2	121	256	15	148	1.39e-25	99.4
MS.gene06476.t1	MTR_1089s0010	33.969	262	146	8	11	258	130	378	1.79e-25	103
MS.gene06476.t1	MTR_5g045510	31.599	269	138	9	7	256	128	369	3.71e-25	102
MS.gene06476.t1	MTR_6g022500	31.679	262	154	9	9	256	223	473	4.96e-25	103
MS.gene06476.t1	MTR_5g058100	31.818	264	155	9	10	258	130	383	7.12e-25	102
MS.gene06476.t1	MTR_3g036250	31.660	259	150	8	7	256	123	363	1.38e-24	100
MS.gene06476.t1	MTR_5g008920	30.943	265	160	8	8	260	129	382	1.50e-24	101
MS.gene06476.t1	MTR_7g007370	36.364	253	137	8	7	251	93	329	2.38e-24	100
MS.gene06476.t1	MTR_3g071850	31.734	271	151	10	6	253	144	403	2.56e-24	102
MS.gene06476.t1	MTR_5g073420	31.559	263	151	7	11	261	137	382	1.32e-23	98.6
MS.gene06476.t1	MTR_7g034115	33.333	237	136	8	6	232	127	351	1.91e-23	98.2
MS.gene06476.t1	MTR_1g108800	34.470	264	138	10	11	256	93	339	2.38e-23	97.4
MS.gene06476.t1	MTR_1g085120	33.459	266	152	9	9	258	214	470	3.64e-23	98.2
MS.gene06476.t1	MTR_5g045170	29.699	266	154	8	11	256	136	388	4.67e-23	97.4
MS.gene06476.t1	MTR_1g085120	33.459	266	152	9	9	258	289	545	5.45e-23	98.2
MS.gene06476.t1	MTR_7g088260	31.343	268	157	9	6	258	206	461	6.75e-23	97.4
MS.gene06476.t1	MTR_6g040270	31.154	260	154	10	11	256	130	378	3.78e-22	94.7
MS.gene06476.t1	MTR_2g036470	32.847	274	144	11	8	256	135	393	5.99e-22	94.4
MS.gene06476.t1	MTR_1g066830	30.909	275	165	8	1	258	120	386	3.40e-21	92.0
MS.gene06476.t1	MTR_5g054920	34.031	191	99	7	54	232	2	177	4.70e-21	88.2
MS.gene06476.t1	MTR_3g109982	32.046	259	156	6	8	257	220	467	5.36e-21	92.4
MS.gene06476.t1	MTR_3g105060	28.030	264	163	8	3	258	128	372	2.35e-20	89.4
MS.gene06476.t1	MTR_5g012840	28.731	268	131	10	4	258	97	317	3.16e-20	88.6
MS.gene06476.t1	MTR_3g087260	32.576	264	157	7	8	258	201	456	3.19e-20	89.7
MS.gene06476.t1	MTR_3g023630	30.153	262	156	9	6	256	126	371	4.30e-20	88.6
MS.gene06476.t1	MTR_0543s0010	29.213	267	159	8	7	258	198	449	9.31e-20	88.2
MS.gene06476.t1	MTR_3g108560	30.534	262	159	8	12	258	135	388	1.26e-19	87.4
MS.gene06476.t1	MTR_4g039440	33.023	215	120	7	31	232	173	376	1.39e-19	87.8
MS.gene06476.t1	MTR_5g073370	30.418	263	168	6	2	256	122	377	1.63e-19	87.4
MS.gene06476.t1	MTR_3g107140	55.696	79	35	0	5	83	119	197	1.92e-19	84.0
MS.gene06476.t1	MTR_5g058000	31.086	267	158	8	4	258	122	374	1.98e-19	87.0
MS.gene06476.t1	MTR_6g022520	31.579	228	128	8	49	258	95	312	2.59e-19	85.9
MS.gene06476.t1	MTR_5g084070	31.655	278	128	10	8	232	126	394	3.01e-19	86.7
MS.gene06476.t1	MTR_7g056020	31.008	258	145	8	12	251	124	366	9.95e-19	85.9
MS.gene06476.t1	MTR_0009s0080	29.880	251	150	8	6	239	136	377	3.07e-18	84.0
MS.gene06476.t1	MTR_1g108820	31.967	244	127	11	11	232	126	352	3.55e-18	83.6
MS.gene06476.t1	MTR_3g450920	54.545	99	35	2	3	99	127	217	4.55e-18	80.9
MS.gene06476.t1	MTR_5g073340	33.333	207	121	6	6	201	100	300	5.98e-18	82.0
MS.gene06476.t1	MTR_5g039450	29.771	262	151	10	16	257	108	356	6.84e-18	82.4
MS.gene06476.t1	MTR_7g056430	30.258	271	133	10	6	253	122	359	1.60e-17	82.4
MS.gene06476.t1	MTR_7g056420	30.258	271	133	10	6	253	122	359	1.71e-17	82.4
MS.gene06476.t1	MTR_5g013500	28.395	243	144	6	39	258	129	364	2.81e-17	80.9
MS.gene06476.t1	MTR_7g056277	28.996	269	159	10	4	258	377	627	3.08e-17	81.3
MS.gene06476.t1	MTR_5g039470	29.412	238	146	8	5	228	98	327	1.13e-16	78.6
MS.gene06476.t1	MTR_0492s0020	29.362	235	142	7	7	232	198	417	1.18e-16	79.3
MS.gene06476.t1	MTR_3g040700	28.261	230	131	6	7	225	161	367	1.46e-16	79.0
MS.gene06476.t1	MTR_5g063950	28.409	264	162	10	11	257	107	360	4.33e-16	77.4
MS.gene06476.t1	MTR_7g056287	28.464	267	163	10	4	258	121	371	6.07e-16	77.0
MS.gene06476.t1	MTR_3g082890	26.761	284	171	9	5	259	209	484	7.26e-16	77.0
MS.gene06476.t1	MTR_7g055943	29.434	265	157	8	16	260	168	422	1.14e-15	76.3
MS.gene06476.t1	MTR_3g071790	29.534	193	106	8	51	226	23	202	2.96e-15	72.8
MS.gene06476.t1	MTR_7g081290	32.143	140	78	5	130	258	122	255	5.04e-15	73.2
MS.gene06476.t1	MTR_1g053050	46.914	81	42	1	180	259	35	115	9.13e-15	69.3
MS.gene06476.t1	MTR_6g011460	30.973	226	143	5	11	232	169	385	1.05e-14	73.6
MS.gene06476.t1	MTR_2g007530	36.646	161	71	5	106	257	197	335	2.46e-14	72.0
MS.gene06476.t1	MTR_8g085430	42.353	85	41	2	153	232	215	296	2.51e-14	72.0
MS.gene06476.t1	MTR_6g044572	29.213	267	150	9	9	262	151	391	2.91e-14	72.0
MS.gene06476.t1	MTR_5g031970	29.730	259	145	10	8	256	122	353	4.13e-14	71.6
MS.gene06476.t1	MTR_6g044560	29.213	267	150	9	9	262	327	567	6.47e-14	71.6
MS.gene06476.t1	MTR_7g021990	29.114	237	148	9	8	232	164	392	6.74e-14	71.2
MS.gene06476.t1	MTR_7g021950	29.114	237	148	9	8	232	164	392	1.05e-13	70.9
MS.gene06476.t1	MTR_5g024060	35.345	116	64	3	120	232	71	178	3.87e-13	67.8
MS.gene06476.t1	MTR_6g022360	30.000	230	144	8	12	232	154	375	3.88e-13	68.9
MS.gene06476.t1	MTR_1377s0010	36.190	105	59	2	130	232	29	127	5.71e-13	65.1
MS.gene06476.t1	MTR_8g467800	26.875	320	166	11	4	258	136	452	5.75e-13	68.6
MS.gene06476.t1	MTR_5g080710	28.276	290	151	9	8	245	149	433	8.44e-13	68.2
MS.gene06476.t1	MTR_4g055580	25.776	322	175	9	2	260	246	566	1.08e-12	67.8
MS.gene06476.t1	MTR_5g039490	28.519	270	140	9	5	256	139	373	4.46e-12	65.9
MS.gene06476.t1	MTR_4g080590	30.088	113	73	2	131	243	324	430	1.14e-11	64.7
MS.gene06476.t1	MTR_3g115430	32.727	110	66	3	152	258	20	124	1.44e-11	60.8
MS.gene06476.t1	MTR_7g114710	25.630	238	121	7	39	258	164	363	1.45e-11	63.9
MS.gene06476.t1	MTR_2g020180	27.891	147	78	4	124	258	84	214	1.92e-11	62.4
MS.gene06476.t1	MTR_1g043170	37.349	83	43	3	182	258	8	87	2.63e-11	58.9
MS.gene06476.t1	MTR_7g022105	27.668	253	165	11	8	249	165	410	3.27e-11	63.2
MS.gene06476.t1	MTR_3g082890	23.899	318	172	9	5	258	129	440	4.17e-11	62.8
MS.gene06476.t1	MTR_5g092960	25.392	319	169	9	7	259	147	462	7.27e-11	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06476.t1	AT3G54910	31.419	296	154	13	8	258	81	372	8.59e-17	79.3
MS.gene06476.t1	AT3G54910	31.419	296	154	13	8	258	81	372	8.59e-17	79.3
MS.gene06476.t1	AT3G54910	31.419	296	154	13	8	258	81	372	8.59e-17	79.3
MS.gene06476.t1	AT4G10420	28.094	299	162	8	8	258	28	321	5.09e-16	76.6
MS.gene06476.t1	AT3G26920	27.338	278	167	10	4	258	35	300	1.32e-15	75.5
MS.gene06476.t1	AT5G62970	28.896	308	159	11	8	259	146	449	2.54e-15	75.5
MS.gene06476.t1	AT5G56820	26.797	306	171	10	6	261	126	428	9.96e-15	73.6
MS.gene06476.t1	AT3G26920	27.885	312	161	14	4	258	35	339	1.17e-14	73.2
MS.gene06476.t1	AT3G26920	27.885	312	161	14	4	258	35	339	1.17e-14	73.2
MS.gene06476.t1	AT4G10400	29.667	300	150	10	8	258	121	408	1.64e-14	73.2
MS.gene06476.t1	AT4G10400	29.667	300	150	10	8	258	121	408	1.64e-14	73.2
MS.gene06476.t1	AT4G10400	29.667	300	150	10	8	258	121	408	1.64e-14	73.2
MS.gene06476.t1	AT4G26340	25.753	299	174	7	8	258	120	418	1.28e-11	64.3
MS.gene06476.t1	AT3G49030	28.125	192	116	7	78	259	262	441	1.40e-11	64.3

Find 39 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGACAATGACCAGGAAAAT+TGG	0.232724	3.2:+79392542	MS.gene06476:CDS
CTATTAGCATTCTTAGTTGC+AGG	0.287668	3.2:+79391802	MS.gene06476:CDS
CATTTAGAAATACAGCTTCT+TGG	0.289681	3.2:-79391908	None:intergenic
TCCAATTCTTGAAGACTTAG+AGG	0.296042	3.2:+79391962	MS.gene06476:CDS
GCCTCTAAGTCTTCAAGAAT+TGG	0.335259	3.2:-79391963	None:intergenic
CAAGCTTTCACACTCTTGAT+AGG	0.339906	3.2:-79392021	None:intergenic
TTGAGGGAGAGGAGTCTAAT+GGG	0.343342	3.2:-79391873	None:intergenic
AGTGAAAGGCATTTCTGCTT+TGG	0.347308	3.2:-79392060	None:intergenic
TTTGAGGGAGAGGAGTCTAA+TGG	0.361072	3.2:-79391874	None:intergenic
CTAACTGAACCTCACCTTGT+TGG	0.365670	3.2:-79392636	None:intergenic
TCGAGATTTACTGTTGCTTT+TGG	0.367267	3.2:+79391932	MS.gene06476:CDS
CTGCAACTAAGAATGCTAAT+AGG	0.394345	3.2:-79391801	None:intergenic
ATACAGCTTCTTGGAAATGA+AGG	0.415286	3.2:-79391899	None:intergenic
AGACAATGACCAGGAAAATT+GGG	0.422035	3.2:+79392543	MS.gene06476:CDS
AGGAGAGGCCTTTGAGCATA+AGG	0.435668	3.2:-79392743	None:intergenic
TAAGAATGCTAATAGGCAAC+TGG	0.438611	3.2:-79391794	None:intergenic
TCAAAATGTTGAGCTTAGTC+AGG	0.442937	3.2:+79392422	MS.gene06476:CDS
ATGCACTGCGGAACAGATGC+AGG	0.449264	3.2:-79392571	None:intergenic
TAGGGCACCGCTGTCGGTGA+TGG	0.451965	3.2:+79392514	MS.gene06476:intron
ACTGTACATAGAAATAAATA+AGG	0.455034	3.2:+79392124	MS.gene06476:CDS
ACCTTAAAACTTGCACACTT+TGG	0.455379	3.2:+79392602	MS.gene06476:CDS
TTTGTCAAATTATAAAATGT+AGG	0.487101	3.2:-79392330	None:intergenic
CTTTCACACTCTTGATAGGA+TGG	0.505112	3.2:-79392017	None:intergenic
TGAGGGAGAGGAGTCTAATG+GGG	0.541200	3.2:-79391872	None:intergenic
GAAAGCACTTTGTAATGTGA+AGG	0.555821	3.2:+79392100	MS.gene06476:CDS
AGGGCACCGCTGTCGGTGAT+GGG	0.561817	3.2:+79392515	MS.gene06476:intron
TATTAATAGGGCACCGCTGT+CGG	0.566394	3.2:+79392508	MS.gene06476:intron
ACATTACAAAGTGCTTTCAA+CGG	0.580486	3.2:-79392095	None:intergenic
GAGGGAGAGGAGTCTAATGG+GGG	0.583077	3.2:-79391871	None:intergenic
AGAAGTCTCCTTATGCTCAA+AGG	0.594523	3.2:+79392735	MS.gene06476:CDS
TGCACACTTTGGTACTTCAG+AGG	0.596491	3.2:+79392613	MS.gene06476:CDS
AATAAGTTCACATGTAGGAG+AGG	0.613500	3.2:-79392758	None:intergenic
CTTCAGAGGCCAACAAGGTG+AGG	0.624723	3.2:+79392627	MS.gene06476:CDS
TGGTACTTCAGAGGCCAACA+AGG	0.648069	3.2:+79392622	MS.gene06476:CDS
TCTTATCCCATCACCGACAG+CGG	0.651040	3.2:-79392521	None:intergenic
TGCACTGCGGAACAGATGCA+GGG	0.654974	3.2:-79392570	None:intergenic
TGGGATAAGAGACAATGACC+AGG	0.655301	3.2:+79392534	MS.gene06476:CDS
AGGATGGAGAGTACTCAGAG+TGG	0.702640	3.2:-79392001	None:intergenic
AGGTGTAATGAAATGCACTG+CGG	0.800716	3.2:-79392583	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGATTTTATCTATATTAATA+GGG	+	chr3.2:79392496-79392515	MS.gene06476:intron	10.0%
!!!	TTGATTTTATCTATATTAAT+AGG	+	chr3.2:79392495-79392514	MS.gene06476:intron	10.0%
!!	AAAATCAAATCAAATCAAAA+AGG	-	chr3.2:79392483-79392502	None:intergenic	15.0%
!!	TTTGTCAAATTATAAAATGT+AGG	-	chr3.2:79392333-79392352	None:intergenic	15.0%
!!!	TTGTTTTTCTTGAATTTTTT+GGG	+	chr3.2:79391829-79391848	MS.gene06476:CDS	15.0%
!!	ACTGTACATAGAAATAAATA+AGG	+	chr3.2:79392124-79392143	MS.gene06476:CDS	20.0%
!!	TAGAATATAATAGAAGAACA+TGG	+	chr3.2:79392186-79392205	MS.gene06476:intron	20.0%
!!!	ATTATGTTGTTTTCTTGTTT+AGG	+	chr3.2:79392284-79392303	MS.gene06476:intron	20.0%
!!!	CTTGTTTTTCTTGAATTTTT+TGG	+	chr3.2:79391828-79391847	MS.gene06476:CDS	20.0%
!	TGAAAAATAAGTTCACATGT+AGG	-	chr3.2:79392766-79392785	None:intergenic	25.0%
	CATTTAGAAATACAGCTTCT+TGG	-	chr3.2:79391911-79391930	None:intergenic	30.0%
	GGAAAATGACAAAAAGTGAA+AGG	-	chr3.2:79392077-79392096	None:intergenic	30.0%
	TAGTCAGGTTTGTAAGTTTA+GGG	+	chr3.2:79392437-79392456	MS.gene06476:intron	30.0%
	TTAGTCAGGTTTGTAAGTTT+AGG	+	chr3.2:79392436-79392455	MS.gene06476:intron	30.0%
	TTTGTATGAATACACTGATG+CGG	+	chr3.2:79392162-79392181	MS.gene06476:intron	30.0%
!	ACATTACAAAGTGCTTTCAA+CGG	-	chr3.2:79392098-79392117	None:intergenic	30.0%
!	GAGATTTTTGACGTTATATC+TGG	+	chr3.2:79392250-79392269	MS.gene06476:intron	30.0%
!	TTGGAAATGAAGGATTTTGA+GGG	-	chr3.2:79391892-79391911	None:intergenic	30.0%
!!!	AGACTTCTCTTTCTTTTTTG+AGG	-	chr3.2:79392723-79392742	None:intergenic	30.0%
!!!	TTTCTTTTTTGAGGCTATCA+CGG	-	chr3.2:79392714-79392733	None:intergenic	30.0%
	AATAAGTTCACATGTAGGAG+AGG	-	chr3.2:79392761-79392780	None:intergenic	35.0%
	ACCTTAAAACTTGCACACTT+TGG	+	chr3.2:79392602-79392621	MS.gene06476:CDS	35.0%
	AGACAATGACCAGGAAAATT+GGG	+	chr3.2:79392543-79392562	MS.gene06476:CDS	35.0%
	ATACAGCTTCTTGGAAATGA+AGG	-	chr3.2:79391902-79391921	None:intergenic	35.0%
	CTATTAGCATTCTTAGTTGC+AGG	+	chr3.2:79391802-79391821	MS.gene06476:CDS	35.0%
	CTGCAACTAAGAATGCTAAT+AGG	-	chr3.2:79391804-79391823	None:intergenic	35.0%
	TAAGAATGCTAATAGGCAAC+TGG	-	chr3.2:79391797-79391816	None:intergenic	35.0%
	TCAAAATGTTGAGCTTAGTC+AGG	+	chr3.2:79392422-79392441	MS.gene06476:CDS	35.0%
	TCCAATTCTTGAAGACTTAG+AGG	+	chr3.2:79391962-79391981	MS.gene06476:CDS	35.0%
!	ACCAAAGTGTGCAAGTTTTA+AGG	-	chr3.2:79392606-79392625	None:intergenic	35.0%
!	CTTGGAAATGAAGGATTTTG+AGG	-	chr3.2:79391893-79391912	None:intergenic	35.0%
!	GACTAAGCTCAACATTTTGA+AGG	-	chr3.2:79392422-79392441	None:intergenic	35.0%
!!	AGCTCAACATTTTGAAGGTT+AGG	-	chr3.2:79392417-79392436	None:intergenic	35.0%
!!	GAAAGCACTTTGTAATGTGA+AGG	+	chr3.2:79392100-79392119	MS.gene06476:CDS	35.0%
!!	GCTCAACATTTTGAAGGTTA+GGG	-	chr3.2:79392416-79392435	None:intergenic	35.0%
!!!	TCGAGATTTACTGTTGCTTT+TGG	+	chr3.2:79391932-79391951	MS.gene06476:CDS	35.0%
	AGAAGTCTCCTTATGCTCAA+AGG	+	chr3.2:79392735-79392754	MS.gene06476:CDS	40.0%
	AGGTGTAATGAAATGCACTG+CGG	-	chr3.2:79392586-79392605	None:intergenic	40.0%
	CAAGCTTTCACACTCTTGAT+AGG	-	chr3.2:79392024-79392043	None:intergenic	40.0%
	CTTTCACACTCTTGATAGGA+TGG	-	chr3.2:79392020-79392039	None:intergenic	40.0%
	GAGACAATGACCAGGAAAAT+TGG	+	chr3.2:79392542-79392561	MS.gene06476:CDS	40.0%
	GCCTCTAAGTCTTCAAGAAT+TGG	-	chr3.2:79391966-79391985	None:intergenic	40.0%
!!	AATGAAGGATTTTGAGGGAG+AGG	-	chr3.2:79391887-79391906	None:intergenic	40.0%
!!	AGTGAAAGGCATTTCTGCTT+TGG	-	chr3.2:79392063-79392082	None:intergenic	40.0%
!!!	GATTTACTGTTGCTTTTGGC+TGG	+	chr3.2:79391936-79391955	MS.gene06476:CDS	40.0%
	CTAACTGAACCTCACCTTGT+TGG	-	chr3.2:79392639-79392658	None:intergenic	45.0%
	TGCACACTTTGGTACTTCAG+AGG	+	chr3.2:79392613-79392632	MS.gene06476:CDS	45.0%
	TGGGATAAGAGACAATGACC+AGG	+	chr3.2:79392534-79392553	MS.gene06476:CDS	45.0%
	TTGAGGGAGAGGAGTCTAAT+GGG	-	chr3.2:79391876-79391895	None:intergenic	45.0%
	TTTGAGGGAGAGGAGTCTAA+TGG	-	chr3.2:79391877-79391896	None:intergenic	45.0%
!!	TATTAATAGGGCACCGCTGT+CGG	+	chr3.2:79392508-79392527	MS.gene06476:intron	45.0%
	AGGAGAGGCCTTTGAGCATA+AGG	-	chr3.2:79392746-79392765	None:intergenic	50.0%
	TCTTATCCCATCACCGACAG+CGG	-	chr3.2:79392524-79392543	None:intergenic	50.0%
	TGAGGGAGAGGAGTCTAATG+GGG	-	chr3.2:79391875-79391894	None:intergenic	50.0%
	TGGTACTTCAGAGGCCAACA+AGG	+	chr3.2:79392622-79392641	MS.gene06476:CDS	50.0%
!	CAGGGTCTACCCAATTTTCC+TGG	-	chr3.2:79392555-79392574	None:intergenic	50.0%
!!	AGGATGGAGAGTACTCAGAG+TGG	-	chr3.2:79392004-79392023	None:intergenic	50.0%
	ATGCACTGCGGAACAGATGC+AGG	-	chr3.2:79392574-79392593	None:intergenic	55.0%
	CTTCAGAGGCCAACAAGGTG+AGG	+	chr3.2:79392627-79392646	MS.gene06476:CDS	55.0%
	GAGGGAGAGGAGTCTAATGG+GGG	-	chr3.2:79391874-79391893	None:intergenic	55.0%
	TGCACTGCGGAACAGATGCA+GGG	-	chr3.2:79392573-79392592	None:intergenic	55.0%
!!	AGGGCACCGCTGTCGGTGAT+GGG	+	chr3.2:79392515-79392534	MS.gene06476:intron	65.0%
!!	TAGGGCACCGCTGTCGGTGA+TGG	+	chr3.2:79392514-79392533	MS.gene06476:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	79391777	79392798	79391777	ID=MS.gene06476
chr3.2	mRNA	79391777	79392798	79391777	ID=MS.gene06476.t1;Parent=MS.gene06476
chr3.2	exon	79391777	79392145	79391777	ID=MS.gene06476.t1.exon1;Parent=MS.gene06476.t1
chr3.2	CDS	79391777	79392145	79391777	ID=cds.MS.gene06476.t1;Parent=MS.gene06476.t1
chr3.2	exon	79392306	79392443	79392306	ID=MS.gene06476.t1.exon2;Parent=MS.gene06476.t1
chr3.2	CDS	79392306	79392443	79392306	ID=cds.MS.gene06476.t1;Parent=MS.gene06476.t1
chr3.2	exon	79392517	79392798	79392517	ID=MS.gene06476.t1.exon3;Parent=MS.gene06476.t1
chr3.2	CDS	79392517	79392798	79392517	ID=cds.MS.gene06476.t1;Parent=MS.gene06476.t1

Gene Sequence

>MS.gene06476

ATGGTTCAAGATGATCTCCAGTTGCCTATTAGCATTCTTAGTTGCAGGACTCTTGTTTTTCTTGAATTTTTTGGGTTCACTGTGAACGTTTCTTCCCCCATTAGACTCCTCTCCCTCAAAATCCTTCATTTCCAAGAAGCTGTATTTCTAAATGTTCGAGATTTACTGTTGCTTTTGGCTGGATGTCCAATTCTTGAAGACTTAGAGGCATCTAGTTTACGATTCCACTCTGAGTACTCTCCATCCTATCAAGAGTGTGAAAGCTTGAACTTAAGCAAGTTAACCAAAGCAGAAATGCCTTTCACTTTTTGTCATTTTCCGTTGAAAGCACTTTGTAATGTGAAGGAACTGTACATAGAAATAAATAAGGTTTGAATTTTATTGTTTTGTATGAATACACTGATGCGGATAGAATATAATAGAAGAACATGGTTGATTTCTATTTTTGTTTTAGCTCTGTTCTTTTTGTTGTTGAGATTTTTGACGTTATATCTGGTAATATATCTTATTATGTTGTTTTCTTGTTTAGGTGTATCGACGTTTTGATGAGATTCCTACATTTTATAATTTGACAAAGTTGAAACTTCACTCTATCAACTATAATTCGCATTTGTTAGTACAAGTGCTCAATCGTTGCCCTAACCTTCAAAATGTTGAGCTTAGTCAGGTTTGTAAGTTTAGGGATTTATTTTTTCTTAACATGCCTTTTTGATTTGATTTGATTTTATCTATATTAATAGGGCACCGCTGTCGGTGATGGGATAAGAGACAATGACCAGGAAAATTGGGTAGACCCTGCATCTGTTCCGCAGTGCATTTCATTACACCTTAAAACTTGCACACTTTGGTACTTCAGAGGCCAACAAGGTGAGGTTCAGTTAGCAACATACATTCTTAATAATGCAAGAGTTTTGCAAACTATGAAAATTTGTTGCCGTGATAGCCTCAAAAAAGAAAGAGAAGTCTCCTTATGCTCAAAGGCCTCTCCTACATGTGAACTTATTTTTCATGTGTTCAAGTGA

Protein sequence

>MS.gene06476.t1

MVQDDLQLPISILSCRTLVFLEFFGFTVNVSSPIRLLSLKILHFQEAVFLNVRDLLLLLAGCPILEDLEASSLRFHSEYSPSYQECESLNLSKLTKAEMPFTFCHFPLKALCNVKELYIEINKVYRRFDEIPTFYNLTKLKLHSINYNSHLLVQVLNRCPNLQNVELSQGTAVGDGIRDNDQENWVDPASVPQCISLHLKTCTLWYFRGQQGEVQLATYILNNARVLQTMKICCRDSLKKEREVSLCSKASPTCELIFHVFK